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C17H21ClN2O, orthorhombic, Pna21 (no. 33), a = 9.2750(10) Å, b = 12.8640(17) Å, c = 13.483(2) Å, β = 90°, V = 1608.8(4) Å3, Z = 4, R gt(F) = 0.0681, wR ref(F 2) = 0.1361, T = 293 K.

Objective The dopamine and oxidative stress hypotheses are leading theories of the pathoetiology of schizophrenia (SCZ). Glutathione Peroxidase 1 (GPx-1), a major antioxidant enzyme, and the most abundantly expressed member of the GPx family, plays an important role in metabolic dopamine changes, which are closely related to neurological and psychiatric disorders. The impact of GPx-1 polymorphisms has rarely been explored in the field of SCZ. Here, we explored the possible relationship between GPx-1 gene polymorphisms and SCZ in Chinese Han subjects by using the polymerase chain reaction-restriction fragment length polymorphism method. Methods DNA from 786 patients (360 patients with schizophrenia and 426 healthy controls) was genotyped for the single-nucleotide polymorphisms rs1800668 C/T and rs1050450 C/T in GPx-1 using polymerase chain reaction-restriction fragment length polymorphism analysis. Analysis of the association between GPx-1 and SCZ was performed using SPSS 22.0, while Haploview 4.2 software and SHEsis software were used to perform linkage disequilibrium analysis and haplotype analysis. Results The results indicated that the GPx-1 polymorphisms rs1050450 and rs1800668 were associated with SCZ. We found that the C-allele of rs1800668 C/T may be a protection factor against SCZ in general, but in particular, for males. Furthermore, the CT and TC (GPx-1 rs1800668 C/T and rs1050450 C/T) haplotypes may be susceptible to SCZ in the population. Finally, no significant differences in allelic or genotypic frequencies of rs1050450 were detected between cases and controls from whole or stratification analyses by gender. Conclusion GPx-1 polymorphisms are related to SCZ in Chinese Han subjects. Our results suggested that GPx-1 may be a potential gene that influences SCZ.

Intraosseous lipomas are rare benign tumors of the bone, especially for the mandible. The etiology remains unclear, although several hypotheses have been proposed, such as trauma, blood vessel infarction, aging and inflammation. Clinically, the symptoms mainly depend on the location and size of the tumors. Radiographically, the lesions are usually radiolucent occasionally accompanied by radiopacity. The diagnosis is challenging, and histopathologic examination is necessary for definite diagnosis. Herein, the authors report an unusual patient with intraosseous lipoma in the mandibular condyle.

Background Glutathione S-transferase (GST) is an important antioxidant enzyme in the body. The weakening of the antioxidant system causes damage to the cells and tissues that make up the organism, adversely affects the function of the nervous system, and ultimately leads to schizophrenia (SCZ). Previous studies have yielded inconsistent results across different ethnic populations. Purpose This case-control study was carried out to investigate whether genetic polymorphisms in GST could be associated with SCZ in the Chinese Han population. Patients and methods A total of 794 participants, including 379 SCZ patients (case group) and 415 healthy individuals (control group), were genotyped by polymerase chain reaction-restriction fragment length for polymorphisms in GST genes. Results The study found that the frequency of the GSTM1 null genotype was higher in case group than control group (p=0.003). The frequency of the GSTM1 and GSTT1 double null genotype was also higher in case group than control group (p=0.008). Conclusion We conclude that the GSTM1 null genotype and the GSTM1 and GSTT1 double null genotype may be related to the onset of SCZ in Chinese Han population.

The risky behaviours of bus drivers are of great concern to public health and environmental sustainability, especially for the buses operated between cities. With this in mind, the present study examined the distribution of risky behaviours among bus drivers, and the contributing factors to risky performance. To achieve this, 1648 records of GPS trajectory data and 8281 records of advance warning message data from Hong Kong–Zhuhai–Macau Bridge shuttle buses were obtained. The temporal and spatial distribution of risky behaviours was analysed. A random parameters negative binomial model was developed to further investigate the relationship between speed-related factors and risky behaviours. The results indicated that the warning of safety distance, lane departure, forward collision, and distraction were more likely to occur on weekdays. The period between 14 and 16 o’clock obtained the highest frequency of safety distance and lane departure warnings. Regarding the model estimation results, indicators reflecting average speed, acceleration, and number of trips per day showed a statistically significant impact on safety distance and lane departure warnings. Also, the acceleration of bus drivers showed a mixed impact on lane departure warnings. Corresponding implications were discussed according to the findings to reduce the frequency of risky behaviours in shuttle bus operations.

Backgrounds: Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) is an lncRNA that has been suggested as a key regulator in the onset of atherosclerosis (AS). This study described the role of MAL-AT1 in oxidized low density lipoprotein (ox-LDL)-induced endothelial cells death. Methods: Human umbilical vein endothelial cells (HU-VECs) were subjected to ox-LDL, before which the expression of MALAT1 was overexpressed by transfection. CCK-8 assay, flow cytometer detection, and western blot were carried out to evaluate cell viability, apoptosis and autophagy. qRT-PCR and western blot analyses were performed to investigate the regulatory relationship between MALAT1, Matrix Gla protein (MGP) and mTOR signaling to decode the underlying mechanism. Results: Up-regulation of MALAT1 attenuated ox-LDL-induced HUVECs lose, as evidenced by the promoted cell viability, and the decreased apoptosis rate. This finding was coupled with the down-regulated p53, Bax, active-caspase-3, Beclin-1 and LC3-II, as well as the up-regulated Bcl-2 and p62. Meanwhile, MALAT1 up-regulation promoted the phosphorylation of p70S6K and mTOR, and the expression of MGP. MGP up-regulation exhibited MALAT1-like propoties in preventing ox-LDL-induced cell death and mTOR deactivation. Of contrast, MGP silence affected HUVECs survival and mTOR signaling resulted in contrary impacts. Conclusion: The present work described that MALAT1 up-regulation prevented ox-LDL-mediated apoptosis and autophagy in HUVECs. The protective effects of MALAT1 might be partially via up-regulating MGP, which led to the activation of mTOR signaling.

Highlight • Children and adolescents who are often neglected in the wake of the outbreak were taken as subjects to investigate their mental health status and analyze the related influencing factors during the global pandemic of COVID-19. • The presence of clinical depressive symptoms, resident in urban regions, implementation of the precaution and control measures, being female, having a family member or friend infected with coronavirus were associated with increased levels of anxiety. • Smartphone addiction, Internet addiction, resident in Hubei province and urban areas, family members or friends infected with coronavirus, graduation affected by the epidemic, levels of separation anxiety, physical injury fear, and tendency to adopt an emotion-focused coping style were associated with increased levels of respondents’ depressive symptoms. • It's urgent to concern and address emotional distress for children and adolescents during the epidemic. Targeted intervention measures could be formulated based on the significant influencing factors on anxiety and clinical depressive symptoms., Objective : The sudden outbreak of Coronavirus Disease 2019 (COVID-19) has had a dramatic effect on the mental health of the public. In the present study, we demonstrated the psychological effects on children and adolescents associated with the epidemic . Methods : By using convenience sampling method, questionnaires, such as Spence Child Anxiety Scale, Child Depression Inventory and Coping style Scale, were distributed to participating 359 children and 3254 adolescents online. Results : The anxiety levels of children and adolescents were (23.87 ± 15.79) and (29.27 ± 19.79), respectively. 22.28% respondents were suffering from depressive symptoms. Seven significant factors associated with increased levels of anxiety, including female, resident in urban regions, emotion-focused coping style. Nine factors associated with increased levels of depression, such as smartphone addiction (OR 1.411, 95% CI 1.099–1.180), Internet addiction (OR 1.844, 95% CI 1.209–2.811), and resident in Hubei province (OR 3.107, 95% CI 1.252–7.708). Two additional factors associated with decreased levels of depressive symptoms: hours spend on Internet per day before the epidemic (OR 0.652, 95% CI 0.609–0.697) and tendency to apply problem-focused coping style (OR 0.937, 95% CI 0.923–0.951). Conclusion : Our findings indicate that the COVID-19 outbreak has had a significant psychosocial impact on children and adolescents. Findings of current levels of anxiety and depression not only highlight the need to address emotional distress for children and adolescents during the epidemic but also provide researchers with scientific fundamentals to formulate targeted interventions based on the significant influencing factors.

Objectives Cloninger developed the three‐dimensional personality theory and Tridimensional Personality Questionnaire (TPQ), which shows that some dimensions of personality traits are heritable and related to neurotransmitters including dopamine. glutathione peroxidase 1 (GPx1) plays an important role in metabolic dopamine change and closely relates to neurological and psychiatric disorders. The impact of GPx‐1 polymorphisms has been rarely explored in the field of personality traits. We decide to explore the relationships between them in healthy Chinese‐Han subjects by using the polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP). Methods In our study, 493 healthy Chinese‐Han participants (male = 234, female = 259) were recruited. 2 ml of EDTA‐treated blood from each volunteer was taken; meanwhile, personality traits were assessed by TPQ. We detected the genotypes of selected two polymorphisms through PCR‐RFLP after extracting DNA. Finally, the association between different genotypes and TPQ scores was performed using SPSS, p, This study is the first to thoroughly investigate associations between GPx‐1 polymorphisms and personality traits. Our results demonstrated that rs1050450 was significantly associated with the disorderliness trait. The rs1800668 polymorphism in GPx‐1 affected the personality trait of impulsiveness in healthy Chinese‐Han subjects.

Social and economic burdens caused by truck-involved rear-end collisions are of great concern to public health and the environment. However, few efforts focused on identifying the difference of impacting factors on injury severity between car-strike-truck and truck-strike-car in rear-end collisions. In light of the above, this study focuses on illustrating the impact of variables associated with injury severity in truck-related rear-end crashes. To this end, truck involved rear-end crashes between 2006 and 2015 in the U.S. were obtained. Three random parameters ordered probit models were developed: two separate models for the car-strike-truck crashes and the truck-strike-car crashes, respectively, and one for the combined dataset. The likelihood ratio test was conducted to evaluate the significance of the difference between the models. The results show that there is a significant difference between car-strike-truck and truck-strike-car crashes in terms of contributing factors towards injury severity. In addition, indicators reflecting male, truck, starting or stopped in the road before a crash, and other vehicles stopped in lane show a mixed impact on injury severity. Corresponding implications were discussed according to the findings to reduce the possibility of severe injury in truck-involved rear-end collisions.

PURPOSE The aim of this meta-analysis was to evaluate the efficacy of open surgery and closed treatment for unilateral moderately displaced mandibular condyle fractures. METHODS PubMed, Embase, and Cochrane Library databases were searched according to PRISMA guidelines from inception to October, 2018. Inclusion criteria were based on humans randomized controlled trials in the English literature. Pertinent data were collected and the incidence of the complications was calculated. RESULTS A total of 6 studies with 227 patients were included in the meta-analysis. The results showed that there were no significant differences in incidence of malocclusion (odds ratio [OR], 0.33; 95% confidence interval [CI]: 0.07-1.46; P = 0.14). However, open surgery group had a better maximal mouth opening (WMD = 3.82, 95% CI: 1.93-5.71, P

Neuroblastoma is the most common tumor diagnosed in children and infants, with high recurrence and poor prognosis. Angelica sinensis polysaccharide (AP) whose average molecular weight is 72,900 Da possesses various bioactivities. We aimed to explore the effects of AP on neuroblastoma SH-SY5Y cells as well as the underlying mechanisms. Effects of AP on cell viability, proliferation, apoptosis, migration, invasion, and expressions of long noncoding RNA H19 (lncRNA-H19), microRNA (miR)-675, and CD44 were assessed. Then, effects of miR-675 overexpression on AP-treated cells were analyzed. Next, expression of key kinases in the PI3K/AKT and JAK/ STAT pathways was detected. The possible target gene of miR-675 was finally explored. Cell viability was reduced by 200-500 µg/mL AP. Meanwhile, AP repressed cell proliferation, migration, and invasion, but induced apoptosis. Expressions of lncRNA-H19 and miR-675 were upregulated in neuroblastoma cells, and were downregulated by AP. AP was also identified to upregulate CD44. We next found AP affected SH-SY5Y cells through downregulating miR-675. Key kinases in the PI3K/AKT and JAK/STAT pathways were downregulated by AP stimulation, while these downregulations were abrogated by miR-675 overexpression. KIF1B isoform β (KIF1Bβ) is proved to be a target of miR-675. In conclusion, AP was first identified to inhibit proliferation, migration, and invasion but induce apoptosis. Furthermore, AP might repress tumorigenesis of SH-SY5Y cells through miR-675-mediated inactivation of the PI3K/AKT and JAK/STAT pathways. Besides, KIF1Bβ might be a target of miR-675.

Neuroblastoma (NB) is a serious disease with high-risk and poor prognosis in children. Survivors often have serious side effects. Angelica polysaccharide (AP) has been proved to exert antitumor function. Therefore, we explored the mechanism of this function in NB to accelerate the clinical application of AP in NB therapy. SH-SY5Y cells were transfected with miR-205 inhibitor and pretreated by AP. Cell activity, colonies number, and apoptosis were detected via Cell Counting Kit-8 assay, colony formation experiment, and flow cytometry, respectively. Targeting link between miR-205 and zinc finger E-box binding homeobox 1 (ZEB1) was measured via luciferase activity assay. Quantitative reverse transcription polymerase chain reaction and western blot was to examine levels of miR-205 and related factors. We found that AP suppressed cell activity and colony formation, whereas induced apoptosis in SH-SY5Y cells. Besides, AP also suppressed Epithelial-mesenchymal transition process and phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) and extracellular signal-regulated kinase (ERK1/2) signal passageways. Additionally, miR-205 was positively regulated by AP. AP played its antitumor functions through up-regulating miR-205. Target of miR-205 was ZEB1. Our study demonstrated that AP played its antitumor role in NB through positively regulation of miR-205, whose target gene was ZEB1.

The basic theory and main features of operating modal analysis method have been elaborated, and then by using this method, the parameters of low-order natural characteristics of some wheeled and tracked self-propelled guns have been obtained. By two examples, the application of the operating modal analysis method for the development of self-propelled gun has been introduced. According to the result of operating modal analysis, for some wheeled self-propelled gun, “beat vibration” between the first-order natural characteristics and firing frequency of the gun has been identified, and further research indicates that the primary cause of the “beat vibration” is that the stiffness of the cradle is too weak which enlarges the vibratory response of the muzzle. With the reinforcement of the cradle, the testing result of firing indicates that the vibratory response of the muzzle is reduced obviously, and that the firing dispersion is much better than before. In order to certificate the accuracy of its dynamic model, for some tracked self-propelled guns, the natural characteristics of the whole gun through a comparison of the operating modal analysis and finite element calculation have been specified, and then according to the results of operating modal analysis, the dynamic model of the gun has been meliorated, and the natural characteristics of the gun by the finite element calculation are consistent with those by the operating modal analysis. This indicates that the model of the gun is correct with the vibratory response of the whole gun under seven running fire simulated, and that the error of vibratory response of the muzzle between firing testing and analog simulation does not exceed 15 %.

In surgical removal of a submandibular gland, trans-cervical approach has esthetic problem and existing trans-oral approaches are extensively invasive. The authors have used trans-oral robotic surgery to remove the submandibular gland with preservation of the sublingual gland and the Wharton's duct, and hereby report the case and discuss our preliminary experiences.

Neuroblastoma is the most common tumor diagnosed in children and infants, with high recurrence and poor prognosis. Angelica sinensis polysaccharide (AP) whose average molecular weight is 72,900 Da possesses various bioactivities. We aimed to explore the effects of AP on neuroblastoma SH-SY5Y cells as well as the underlying mechanisms. Effects of AP on cell viability, proliferation, apoptosis, migration, invasion, and expressions of long noncoding RNA H19 (lncRNA-H19), microRNA (miR)-675, and CD44 were assessed. Then, effects of miR-675 overexpression on AP-treated cells were analyzed. Next, expression of key kinases in the PI3K/AKT and JAK/ STAT pathways was detected. The possible target gene of miR-675 was finally explored. Cell viability was reduced by 200-500 µg/mL AP. Meanwhile, AP repressed cell proliferation, migration, and invasion, but induced apoptosis. Expressions of lncRNA-H19 and miR-675 were upregulated in neuroblastoma cells, and were downregulated by AP. AP was also identified to upregulate CD44. We next found AP affected SH-SY5Y cells through downregulating miR-675. Key kinases in the PI3K/AKT and JAK/STAT pathways were downregulated by AP stimulation, while these downregulations were abrogated by miR-675 overexpression. KIF1B isoform β (KIF1Bβ) is proved to be a target of miR-675. In conclusion, AP was first identified to inhibit proliferation, migration, and invasion but induce apoptosis. Furthermore, AP might repress tumorigenesis of SH-SY5Y cells through miR-675-mediated inactivation of the PI3K/AKT and JAK/STAT pathways. Besides, KIF1Bβ might be a target of miR-675.

Neuroblastoma (NB) is a serious disease with high-risk and poor prognosis in children. Survivors often have serious side effects. Angelica polysaccharide (AP) has been proved to exert antitumor function. Therefore, we explored the mechanism of this function in NB to accelerate the clinical application of AP in NB therapy. SH-SY5Y cells were transfected with miR-205 inhibitor and pretreated by AP. Cell activity, colonies number, and apoptosis were detected via Cell Counting Kit-8 assay, colony formation experiment, and flow cytometry, respectively. Targeting link between miR-205 and zinc finger E-box binding homeobox 1 (ZEB1) was measured via luciferase activity assay. Quantitative reverse transcription polymerase chain reaction and western blot was to examine levels of miR-205 and related factors. We found that AP suppressed cell activity and colony formation, whereas induced apoptosis in SH-SY5Y cells. Besides, AP also suppressed Epithelial-mesenchymal transition process and phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) and extracellular signal-regulated kinase (ERK1/2) signal passageways. Additionally, miR-205 was positively regulated by AP. AP played its antitumor functions through up-regulating miR-205. Target of miR-205 was ZEB1. Our study demonstrated that AP played its antitumor role in NB through positively regulation of miR-205, whose target gene was ZEB1.