Search

Abstract Objective To investigate the impact of vitamin A (VA), vitamin D (VD), and homocysteine (Hcy) on cardiometabolic multimorbidity (CMM). Methods This study is a cross-sectional study conducted in Ningxia Province, China. A total of 5000 participants aged 25–74 were recruited and divided into two groups based on the definition of cardiometabolic multimorbidity: the CMM group and the Non CMM group. Demographic, lifestyle, and laboratory data were collected to investigate the correlation between vitamin A, D, Hcy levels and CMM risk. The association was analyzed using multiple logistic regression and restricted cubic spline method. Results CMM incidence increased with age, being higher in females (20.05%) compared to males, Hypertension was present in 96.20% of CMM cases. Reduced VD levels correlated with an elevated CMM risk (OR = 1.799, 95% CI: 1.466–2.238), showing an inverse dose-response relationship, even after adjusting for confounders (OR = 1.553, 95% CI: 1.233–1.956). However, VA and Hcy levels were not significantly associated with CMM risk. The inverse correlation between VD status and CMM risk was more pronounced in males, obese individuals, and those with normal blood lipid profiles (P

Abstract Background Due to its unique anatomical characteristics, supracondylar fractures of the humerus are often difficult to achieve firm fixation with internal fixation equipment, resulting in delayed functional exercise, often leaving cubitus varus deformity, elbow stiffness, contractures, and other complications. Here, we report an adult patient with a supracondylar fracture of the humerus who underwent internal fixation through an anterior median incision in the humerus with our self-developed anterior anatomical locking plate of the distal humerus. Case presentation A 29-year-old male patient of Chinese ethnicity with trauma-induced right supracondylar fracture of the humerus and multiple soft tissue contusions, without nerve damage, blood vessel damage, or other injuries, underwent an internal incision in our hospital using a new anatomical locking plate for the anterior distal humerus fixed treatment. During the 16-month follow-up period, the patient’s elbow range of motion was almost completely restored, functional scores were excellent, and there were no minor or major postoperative complications. Conclusion In this study, we propose a surgical reconstruction strategy for adult patients with supracondylar humeral fractures. Through the anterior median incision of the humerus, open reduction and internal fixation were performed with an anatomic locking plate on the anterior side of the distal humerus to restore and fix the structure of the distal humerus, and satisfactory clinical results were achieved in our case.

PI3Kδ is a key signal transduction molecule in normal and malignant B cells, as well as in T-regulatory cells, making it a promising target for treatment of hematologic malignancies through both direct killing and anti-tumor immunity regulation. BGB-10188 is a highly selective inhibitor of PI3Kδ, showing more than 3000 folds selectivity over other PI3K isoforms and no significant inhibition across tested kinases. BGB-10188 potently inhibited PI3Kδ with IC50s ranging from 1.7-16 nM through various in vitro assays and showed a long-lasting and strong target inhibition in mouse B cells in vivo. BGB-10188 showed significant antitumor effects in human B cell lymphoma xenograft models as single agent or in combination with the BTK inhibitor zanubrutinib. BGB-10188 showed significant Treg inhibition in blood but not in colon, along with less drug accumulation in colon compared with idelalisib, which is an approved PI3Kdelta inhibitor with high incidence of gastrointestinal side effects in clinic. In summary, BGB-10188 is a novel PI3Kδ inhibitor with high selectivity, potency and improved safety profile shown in preclinical studies, which is showing the potential as a best-in-class PI3Kδ inhibitor.

Background Axial symptoms (AS) represent one of the primary complications after cervical spondylotic myelopathy (CSM) surgery. Traditional Chinese Medicine (TCM), as a complementary and alternative therapy, is widely used in the treatment of postoperative AS in CSM. However, it lacks standardised treatment protocols, uniform therapeutic criteria, assessment methods and a sufficiently in-depth understanding of its mechanisms of action. These shortcomings impact the credibility of TCM treatment in clinical practice.Methods and analysis We will conduct comprehensive searches, both manual and electronic, on literature published up to 31 July 2024, and database searches will commence after the publication of this agreement, with an estimated commencement date of 1 October 2024 and the end date of 31 March 2025, without language restrictions. Key databases such as MEDLINE, PubMed, Embase, Web of Science, Cochrane Library, WHO International Clinical Trial Registration Platform, China National Knowledge Network, China Biomedical Literature Database, China Scientific Journal Database and Wanfang Database will be explored. In addition, we will include resources such as library journals and conference abstracts. After identifying and screening all randomised controlled trials focused on TCM for postoperative AS of CSM, the two investigators will conduct a meta-analysis of the included studies. The results will be summarised as the risk ratio for binary data and the standardised or weighted average difference for continuous data.Ethics and dissemination Ethical approval is not required since this review does not involve individual patient data. The review’s findings will provide clinicians with evidence on using TCM treatment for AS post-CSM surgery, disseminated through peer-reviewed publications or conferences.PROSPERO registration number CRD42024505160.

BackgroundEvidence shows that the total flavonoids of Rhizoma Drynariae (TFRD) can improve bone mineral density (BMD). However, there is no evidence to summarize the improvement of biochemical indicators of bone metabolism (BIBM).MethodsThe PubMed, Web of Science, Cochrane Library, Embase, Chinese National Knowledge Infrastructure (CNKI), Wanfang Database, Chongqing VIP Information Database (VIP) and SinoMed were searched from inception to 6 May 2024. The final included studies performed meta-analyses using RevMan 5.3.ResultsNine randomized controlled trials (RCTs) were ultimately included. The TFRD group had higher bone gla protein (BGP) and type I procollagen-N-propeptide (PINP) compared to the Other therapies (WMD: 5.11; 95% CI: 3.37, 6.84; p < 0.00001; WMD: 13.89; 95% CI: 11.81, 15.97; p < 0.00001). The tartrate-resistant acid phosphatase (TRACP) decreased significantly (WMD: −1.34; 95% CI: −1.62, −1.06; p < 0.00001). The alkaline phosphatase (ALP) increased significantly (WMD: 7.47; 95% CI: 6.29, 8.66; p < 0.00001). There were no significant differences in serum calcium (SC) or serum phosphorus (SP) levels between the TFRD and control groups (WMD: 0.08; 95% CI: −0.04, 0.20; p = 0.17; WMD: 0.02; 95% CI: −0.02, 0.05; p = 0.36).ConclusionTFRD can stimulate bone formation and prevent bone resorption in osteoporosis (OP) patients, but it has no effect on SC and SP.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/.

Introduction Osteoporotic vertebral compression fracture (OVCF) is a common complication in elderly patients with osteoporosis. Despite undergoing percutaneous kyphoplasty (PKP) treatment, a significant percentage of OVCF patients (1.8% to 31.9%) continue to experience residual low back pain. While acupuncture has shown promise in relieving this pain, there is currently no systematic review on its efficacy specifically for residual low back pain after PKP in OVCF patients. This project aims to evaluate the effectiveness and safety of acupuncture as a treatment for this condition.Methods and analysis A comprehensive search will be conducted, including manual and electronic searches of literature published. Various databases such as MEDLINE, PubMed, EMBASE, Web of Science, Cochrane Library, International Clinical Trial Registration Platform, China National Knowledge Network, China Biomedical Literature Database, China Scientific Journal Database and Wan-fang Database will be explored. Additional sources like bibliographies and meeting minutes will also be searched. All randomised controlled clinical trials related to acupuncture for treating residual low back pain after PKP in OVCF patients will be included. Two researchers will independently perform study selection, data extraction and quality assessment. The primary outcome measure will be pain relief assessed using a visual analogue scale (VAS) or other validated scales. Secondary outcomes include effectiveness, Oswestry dysfunction index (ODI), quality of life questionnaire (QUALEFFO-41), follow-up relapse rate and adverse events. If feasible, a meta-analysis using RevMan V.5.3 software will be conducted. Otherwise, descriptive or subgroup analyses will be performed. Database searches will commence after the publication of this agreement, with an estimated commencement date of 1 August 2024.Ethics and dissemination Ethical approval is not required since this review does not involve individual patient data. The findings will be disseminated through peer-reviewed journals or relevant conferences.PROSPERO registration number CRD42023478838.

The usage of cheap crude H2 in proton-exchange membrane fuel cells (PEMFCs) is still unrealistic to date, due to the suffering of the current Pt based nano-catalysts from impurities such as CO in anode. Recently, synergistic active sites between single atom (SA) and nanoparticle (NP) have been found to be promising for overcoming the poisoning problem. However, lengthening the nanoparticle-single atom (SA–NP) interface, i.e., constructing high density synergistic active sites, remains highly challenging. Herein, we present a new strategy based on molecular fusion strategy to create abundant SA–NP interfaces, with high density SA–NP interfaces created on a two dimensional nitrogen doped carbon nanosheets (Ir-SACs&NPs/NC). Owing to the abundance of SA–NP interface sites, the catalyst was empowered with a high tolerance towards up to 1000 ​ppm CO in H2 feed. These findings provide guidelines for the design and construction of active and anti-poisoning catalysts for PEMFC anode.

Thymosin beta 10 (TMSB10) overexpression is a general characteristic in human carcinogenesis. It is involved in the malignant process of generating multiple cancers. However, there are only a few reports about TMSB10 in colorectal cancer (CRC) and the mechanism of its carcinogenetic effect is still poorly understood. The present study intends to clarify the biological roles and carcinogenic mechanism of TMSB10 in CRC and to explore the possibility whether TMSB10 might be useful as a non-invasive serum tumor biomarker in detecting CRC. Immunohistochemical results showed that TMSB10 protein expression in CRC tissues was generally higher than that in adjacent tissues, and the TMSB10 contents in serum of CRC patients was significantly elevated compared to that of healthy controls. Knockdown-TMSB10 increased apoptosis and induced S-cell cycle arrest, and finally inhibited cell proliferation in vitro and in vivo. Transcriptome sequencing and western blotting analysis revealed that knockdown-TMSB10 increased phosphorylation of p38 and activated the p38 pathway that blocked cell cycle and promoted apoptosis. Taken together, our study indicated that TMSB10 could serve as a minimally invasive serum tumor marker in detecting CRC. At the same time it demonstrates an effective regulatory capacity of TMSB10 on cell proliferation of CRC, suggesting that TMSB10 and downstream effector molecules regulated by TMSB10 could further be applied as an appealing target in clinical post-surgery chemotherapy.

Parthenocarpic fruits, known for their superior taste and reliable yields in adverse conditions, develop without the need for fertilization or pollination. Exploring the physiological and molecular mechanisms behind parthenocarpic fruit development holds both theoretical and practical significance, making it a crucial area of study. This review examines how plant hormones and MADS-box transcription factors control parthenocarpic fruit formation. It delves into various aspects of plant hormones-including auxin, gibberellic acid, cytokinins, ethylene, and abscisic acid—ranging from external application to biosynthesis, metabolism, signaling pathways, and their interplay in influencing parthenocarpic fruit development. The review also explores the involvement of MADS family gene functions in these processes. Lastly, we highlight existing knowledge gaps and propose directions for future research on parthenocarpy.

Intervertebral disc degeneration (IDD) is widely recognized as the primary culprit of chronic low back pain. Restoring deteriorated intervertebral disc (IVD) and alleviating IDD‐induced low back pain are remaining enormous challenges. There is a genetic susceptibility to IDD, and gene therapy has some therapeutic potential. However, traditional gene therapy still has certain drawbacks, including host immunity, temporary release, and suppression of gene medication function. Although regenerative materials can effectively improve the local microenvironment, they cannot treat IDD from the root. Gene‐functionalized regenerative material (GRM) is constructed based on physical embedding or forming chemical bonds by introducing particular genes, such as pDNA, siRNA, mRNA, and miRNA, into the regenerated materials. The findings demonstrate that GRM not only enhances the safety and controllability of gene therapy, but also effectively repairs IDD by overcoming the constraint that simple regenerative materials cannot reverse the disease's progression from the root. This article provided a brief overview of the physiological and pathological features of the IVD, genetic susceptibility to IDD, available treatment options, and their limitations. Then, the significance of GRM‐based treatment of IDD is proposed, and the future challenges and development in this field are finally prospected.

Abstract Background Glutathione S-transferases (GSTs) are a superfamily of multifunctional enzymes in living organisms with metabolic and detoxification functions, which can detoxify exogenous and endogenous compounds and thereby reduce the damage caused by toxic substances to the body. Ticks are obligate blood-sucking ectoparasites that can transmit various pathogens, and the characterization of tick-derived GSTs may help improve current understanding of the molecular mechanism of tick resistance to insecticides. In this study, a novel GST gene, named HrGSTm1, was identified from Hyalomma rufipes. Methods Sequence analysis was performed by using bioinformatics techniques. A prokaryotic expression system was used to obtain the recombinant expression protein rHrGSTm1. Detection of spatiotemporal expression patterns of target genes and their response to the toxicity of cyhalothrin on female H. rufipes was performed by using a quantitative PCR platform. The optimal enzymological parameters of rHrGSTm1 using glutathione as substrate were calculated. The antioxidant capacity of the recombinant protein was evaluated by DPPH• (1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl). Knockdown of the HrGSTm1 genes through RNA interference was used to analyze their effects on the physiological parameters of ticks. The changes in HrGSTm1 messenger RNA expression patterns under cypermethrin stress were analyzed. Results The complementary DNA sequence of HrGSTm1 contained a 672-bp open reading frame, which potentially encoded 223 amino acids. The predicted molecular weight was 25.62 kDa, and the isoelectric point 8.22. HrGSTm1 is a Mu-class GST, belonging to the cytoplasmic GSTs with no signal peptide observed. The V max and K m of rHrGSTm1 were 3.367 ± 0.81 uM and 2.208 ± 0.76 uM, respectively, and its activities were dependent on different temperatures and pH conditions; the scavenging rate of rHrGSTm1 to DPPH• reached 76.4% at 1.25 mg/ml. Variable expressions of HrGSTm1 were observed under various treatment periods and in different tissues, with the highest appearing in eggs (analysis of variance [ANOVA], F (2, 9) = 279.9, P

Abstract Background Recurrent lateral patellar dislocation (RLPD) poses a significant threat to patients’ quality of life due to knee pain, patellofemoral cartilage damage, and potential traumatic arthritis. Predictive scoring systems have been developed to assess the risk of RLPD; however, their relative accuracy remains uncertain. Purpose To investigate the accuracy of the multiple regression models to predict the individual risk of recurrent LPD. Methods The Patellar Instability probability calculator (PIP), Recurrent Instability of the Patella Score (RIP), and Patellar Instability Severity Score (PIS) scoring rules were measured in 171 patients with a history of patellar dislocation and 171 healthy individuals. Three prediction models were calculated based on the data to predict the risk of recurrent lateral patellar dislocation. The inter-observer and intra-observer reliability of each measurement parameter was evaluated. The predictive capacity of the three-prediction model was investigated using the receiver operating characteristic curve. Results In the case group of 171 patients, PIS accurately predicted recurrent lateral Patella dislocation in 143 patients. RIP was 96, and PIP was 83. The positive predictive values were 92.9%, 64%, and 68% respectively. In the control group of 171 patients, the PIS was validated in 160 patients who would not experience dislocations. RIP was 117, and PIP was 50. The negative predictive values were 85.1%, 60.9%, and 36.2%, respectively. The area under the curve score for the PIS was 0.866, and the RIP was 0.673. the PIP was 0.678. Conclusion RIP and PIP did not work to predict LPD. PIS can accurately predict recurrent lateral patellar dislocation. It can aid doctors in making treatment decisions. Level of evidence Level III, retrospective comparative study.

Gasdermin D (GSDMD) is a key executor of pyroptosis, a form of inflammation-induced programmed cell death. Recently, GSDMD has been shown to play important roles in the development of various inflammatory-related human diseases including heart failure and cancer, suggesting that it is a promising therapeutic target for these diseases. While extensive studies on GSDMD’s role in pyroptosis have been reported, it is challenging to study its function due to the lack of enzymatic activity of GSDMD. In this study, we used the NanoBiT technology to develop a novel GSDMD bioluminescent biosensor (GSDMD-BS) that detects the amount of non-cleaved GSDMD. This sensor allows us to quantify GSDMD’s intramolecular interactions, the amounts of uncleaved GSDMD after caspase-1 cleavage, and expression levels in living cells. In vitro experiments using purified GSDMD-BS also confirmed the sensor’s accuracy in reporting GSDMD levels and cleavage. Moreover, the potential for in vivo application was demonstrated in a xenograft mouse model. In conclusion, we have developed a GSDMD biosensor that is a valuable tool for real-time monitoring of GSDMD dynamics and pyroptosis. This biosensor will significantly expedite pyroptosis research and can be used for high-throughput screening for drugs targeting GSDMD for the therapy of many inflammation-related diseases.

To enhance the service life of traditional asphalt pavement and mitigate issues such as high-temperature rutting and low-temperature cracking, this study investigates the composite modification of matrix asphalt using thermoplastic polyurethane (TPU) and styrene-butadiene rubber (SBR). Initially, the study examines the conventional properties of the composite-modified asphalt from a macro perspective, analyzing the performance variations of asphalt before and after TPU and SBR modification. Subsequently, microscopic analysis is conducted to explore the microstructure, phase structure, and modification mechanisms of the composite-modified asphalt, with a focus on understanding the underlying reasons for performance changes. The influence of TPU and SBR on asphalt performance is evaluated comprehensively. It is found that TPU-modified asphalt demonstrates superior high-temperature performance, storage stability, and elastic recovery. Conversely, SBR-modified asphalt excels in ductility at low temperatures, though its storage stability decreases with increasing dosage. Based on a thorough analysis of the conventional properties of the two types of modified asphalt, the optimal dosages of TPU and SBR are determined to be 15% and 3.5%, respectively. In the composite-modified asphalt, TPU facilitates the even distribution of chemical components, creating a more stable cross-linked network structure. The compatibility of TPU, SBR, and asphalt contributes to the good storage stability of the composite-modified asphalt. While SBR effects physical modification, TPU induces chemical modification of asphalt. Consequently, the composite modification system benefits from both physical and chemical enhancements, resulting in excellent overall performance.

To mitigate the impact of wind power uncertainty and power–communication coupling on the robustness of a new power system, a bi-level mixed-integer robust optimization strategy is proposed. Firstly, a coupled network model is constructed based on complex network theory, taking into account the coupled relationship of energy supply and control dependencies between the power and communication networks. Next, a bi-level mixed-integer robust optimization model is developed to improve power system resilience, incorporating constraints related to the coupling strength, electrical characteristics, and traffic characteristics of the information network. The upper-level model seeks to minimize load shedding by optimizing DC power flow using fuzzy chance constraints, thereby reducing the risk of power imbalances caused by random fluctuations in wind power generation. Furthermore, the deterministic power balance constraints are relaxed into inequality constraints that account for wind power forecasting errors through fuzzy variables. The lower-level model focuses on minimizing traffic load shedding by establishing a topology–function-constrained information network traffic model based on the maximum flow principle in graph theory, thereby improving the efficiency of network flow transmission. Finally, a modified IEEE 39-bus test system with intermittent wind power is used as a case study. Random attack simulations demonstrate that, under the highest link failure rate and wind power penetration, Model 2 outperforms Model 1 by reducing the load loss ratio by 23.6% and improving the node survival ratio by 5.3%.

We conducted an analysis on the combined effects of two light conditions (L1: greenhouse natural lighting; L2: greenhouse natural lighting plus supplemental lighting (SL)) and three nutrient solution concentrations (EC, NS1: 3.2 dS/m; NS2: 3.7 dS/m; NS3: 4.2 dS/m) on the growth, fruit production, and quality of two cherry tomato cultivars with different fruit coloring (‘Baiyu’ and ‘Qianxi’). The plants subjected to NS2 exhibited enhanced growth, photosynthetic parameters, and fruit production. The utilization of SL further enhanced stem diameter, leaf number, and single fruit weight, resulting in higher fruit weight per plant in ‘Baiyu’, which was not observed in ‘Qianxi’. The growth, fruit size, and fruit weight of both cultivars cultivated under NS3 conditions were suppressed, while these fruits exhibited elevated levels of total soluble solids (TSS), soluble sugars, vitamin C, polyphenols, fructose, glucose, sucrose, citric acid, and carotenoids. These levels were further enhanced by SL treatment. The improvement of fruit quality through the application of SL was found to be cultivar and EC dependent. In ‘Baiyu’, SL at NS1 significantly enhanced the accumulation of fruit water, minerals (N, P, K, Ca, and Mg), TSS, vitamin C, fructose, sucrose, and carotenoids. However, this effect was not observed in ‘Qianxi’. The combination of SL and EC 4.2 dS/m (NS3) generally contributes to the enhancement of fruit quality, while SL and EC 3.7 dS/m can ensure consistent fruit production. The yellowish-white fruit cultivar exhibited higher levels of soluble sugars, vitamin C, and polyphenols under L2NS3 conditions compared to the red fruit cultivar, whereas the carotenoid content showed an opposite trend. The findings are anticipated to establish a theoretical foundation for the consistent annual cultivation of cherry tomatoes in protected horticultural settings.

Nano-organic montmorillonite (OMMT) not only inhibits the harmful asphalt fume generation during the production and construction processes of asphalt mixtures but also effectively improves the performance of asphalt pavements. In order to prepare asphalt materials with smoke suppression effects and good road performance, this study selects nano-OMMT and SBS-modified asphalt for composite modification of asphalt mixtures and systematically investigates its road performance. Through the temperature sweep test, the frequency sweep test, the multiple stress creep recovery (MSCR) test, the bending beam rheometer (BBR) test, and the atomic force microscope (AFM) test, the high-temperature rheological properties, low-temperature rheological properties, high-temperature properties and aging resistance of the modified asphalt are studied. The research findings indicate that OMMT can effectively reduce the sensitivity of modified asphalt to load stress and improve its high-temperature rheological properties. SBS-modified asphalt shows increased creep stiffness and a decreased creep rate after OMMT modification, resulting in reduced flexibility and decreased low-temperature crack resistance. After short-term and long-term aging, the complex modulus aging index of OMMT/SBS composite-modified asphalt is lower than that of SBS-modified asphalt, and the phase angle aging index is higher than that of SBS-modified asphalt, demonstrating that OMMT enhances the aging resistance of SBS-modified asphalt. OMMT inhibits oxidation reactions in the asphalt matrix, reducing the formation of C=O and S=O bonds, thereby slowing down the aging process of modified asphalt and improving its aging resistance.

Abstract Preventing the accretion of droplets on surfaces is vital and slippery liquid‐infused porous surfaces (SLIPS) have promising application prospects, such as surface self‐cleaning and droplet transportation. In this work, controllable self‐transport of bouncing droplets on ultraslippery surfaces with wedge‐shaped grooves is reported. The impact behaviors of droplets on SLIPS under various impact velocities and diameters are explored, which can be classified as hover, total bounce, partial bounce, Worthington jet, and crush. SLIPS with wedge‐shaped grooves were designed to transport accreted droplets. An energy and transport model is established to explain the impact and self‐transport mechanism, where the Laplace pressure and moving resistance between droplets play a key role. Finally, SLIPS with branched wedge‐shaped grooves were designed for droplet self‐transport and demonstrated advantages. This work provides a general reference for spontaneous motion control of sessile droplets, droplets with initial impacting velocity, or even liquid films.

Abstract Objectives Vascular stiffening is highly predictive of major adverse cardiovascular events. It is not clear whether microangiopathy, such as fundus arteriosclerosis, is related to carotid atherosclerosis. Hence, this study was designed to investigate the relationship between carotid atherosclerosis and fundus arteriosclerosis among individuals of different sexes in the Chinese health-examination population. Methods This retrospective cross-sectional study involved 20,836 participants, including 13050 males and 7786 females. All participants underwent a detailed health examination, including medical history assessment, physical examination, assessment of lifestyle factors, fundus photography, Doppler ultrasound examination of the neck, and laboratory examinations. Two trained ophthalmologists analysed fundus arteriosclerosis based on fundus photographs, while carotid atherosclerosis was diagnosed using colour Doppler sonography of the neck. Binary logistic regression was used to analyse the relationship between carotid atherosclerosis and fundus arteriosclerosis. Results In participants with fundus arteriosclerosis, the incidence of carotid atherosclerosis was higher than that of participants without fundus arteriosclerosis (52.94% vs. 47.06%). After adjustments for potential confounding factors, fundus arteriosclerosis was significantly associated with the risk of carotid atherosclerosis. The OR with 95% CI for fundus arteriosclerosis was 1.17 (1.02, 1.34) with p = 0.0262, and individuals who did not have fundus arteriosclerosis were used as a reference in the total population. Fundus arteriosclerosis was associated with the incidence of carotid atherosclerosis in males (p = 0.0005) but not in females (p = 0.0746). Conclusions Fundus arteriosclerosis was closely associated with carotid atherosclerosis in the Chinese population. This association was found in males but not in females.

In this paper, the theoretical innovation is made on the traditional fire distribution problem. Considering the actual situation of the ship’s fault, based on the principle of multi-objective optimization, the improved distributed genetic algorithm is used for preliminary solution. And the corresponding fault-tolerant control law is formulated to correct the fault-tolerant strategy, so as to ensure the better fire strike effect and maintain a high fault tolerance. Finally, through simulation experiments, it is verified that this method can converge to the optimal solution quickly, and can reduce the loss to the maximum extent when compensating. It has certain research value for combat.

【Objective】The yield and quality of different varieties of cherry tomatoes (Solanum lycopersicum var. cerasiforme) were compared and analyzed under the substrate bag cultivation mode in multi-span greenhouse in South China, with a view to screening suitable high-quality variety and providing theoretical references for production.【Method】We compared and analyzed the growth, single fruit weight, antioxidant capacity, and fruit quality indexes of 6 cherry tomato varieties ('Qianxi', 'Jinyu', 'Baifei', 'Micai', 'Jinyu' and 'Zhanma'), including the contents of soluble solids, vitamin C (Vc), lycopene, total soluble sugar, etc. And principal component analysis, cluster analysis, and correlation analysis were conducted for the above indexes.【Result】The first cluster of the 'Qianxi' and 'Zhanma' bloomed about 3-6 d earlier than that of other varieties, and the 'Zhanma' had the longest flowering period among 6 varieties, and its flowering period of the first and the second clusters lasted for about 37 d. Moreover, the growth vigor of 'Zhanma' was the strongest with lots of leaflets, and its plant height was higher than that of 'Feicui', 'Qianxi', 'Micai', 'Jinyu' and 'Baifei' by 54.8%, 34.7%, 23.4%, 17.2% and 13.9%, respectively. In contrast, 'Feicui' showed short and thick stem, but single fruit weight and single plant yield were significantly higher than those of other varieties. The yield of 'Micai' and 'Zhanma' was higher than that of others, presenting high fruit quantity in each cluster, approximately up to 24 and 32 in each cluster, respectively. There were significant differences in the quality among 6 varieties of cherry tomato. The contents of chlorophyll, carotenoid and lycopene in the fruit of 'Micai' were highest, up to 33.7, 86.1 and 74.5 μg/g, respectively. The fruits of 'Micai' and 'Zhanma' had the highest Vc contents, which were up to 200 and 220 μg/g, respectively. The fruit of 'Jinyu' variety was rich in polyphenols. The quality of the 'Feicui' was relatively poor. The results of principal component analysis, clustering analysis and correlation analysis indicated that the comprehensive evaluation of 'Micai' variety was the best, followed by 'Qianxi' and 'Zhanma', and then by 'Baifei' and 'Jinyu', and that of 'Feicui' variety was the worst. Fruit size had a significant negative correlation with fruit quality, chlorophyll content and fruit quality (carotenoid, lycopene, flavonoids and Vc), while the chlorophyll content of various varieties of cherry tomato was positively correlated with fruit quality (carotenoid, lycopene, flavonoid, Vc content).【Conclusion】The comprehensive evaluation results showed that the 'Micai' and 'Zhanma' varieties had more fruit number in one cluster and higher yield, and the 'Qianxi' had better flavor quality, the comprehensive evaluation of the three varieties was excellent, and were suitable for high-quality and high-yield cherry tomato varieties in the multi-greenhouse matrix bag culture in South China. Mature 'Micai' cherry tomato is rich in chlorophyll, carotenoid and lycopene, which can be used as the variety of cherry tomato with high pigment content as the ideal material in cultivation.

Abstract Exosomal PD-L1 (exoPD-L1) has recently received significant attention as a biomarker predicting immunotherapeutic responses involving the PD1/PD-L1 pathway. However, current technologies for exosomal analysis rely primarily on bulk measurements that do not consider the heterogeneity found within exosomal subpopulations. Here, we present a nanoscale cytometry platform NanoEPIC, enabling phenotypic sorting and exoPD-L1 profiling from blood plasma. We highlight the efficacy of NanoEPIC in monitoring anti-PD-1 immunotherapy through the interrogation of exoPD-L1. NanoEPIC generates signature exoPD-L1 patterns in responders and non-responders. In mice treated with PD1-targeted immunotherapy, exoPD-L1 is correlated with tumor growth, PD-L1 burden in tumors, and the immune suppression of CD8+ tumor-infiltrating lymphocytes. Small extracellular vesicles (sEVs) with different PD-L1 expression levels display distinctive inhibitory effects on CD8 + T cells. NanoEPIC offers robust, high-throughput profiling of exosomal markers, enabling sEV subpopulation analysis. This platform holds the potential for enhanced cancer screening, personalized treatment, and therapeutic response monitoring.

Abstract This article presents a dual‐band filtering power amplifier (DB‐FPA) with extended passband bandwidths and wide stopband rejection, which is helpful in reducing the number of microwave components in the dual‐band radio frequency front end, resulting in compact circuit size and low loss. In the designed DB‐FPA, a wideband low‐pass filter is applied as the input matching network (IMN) to provide wideband impedance matching and good stopband rejection, while a dual‐band filtering impedance transformation is adopted as the output matching network (OMN) to provide dual‐band impedance matchings and filtering responses simultaneously. Specifically, the IMN is constructed by high‐impedance transmission‐line sections and low‐impedance open‐circuit stubs. The OMN is designed based on the specific external quality factors and coupling coefficients during two bands, resulting in dual‐band impedance transformations and dual‐band filtering responses. Then, based on the above IMN and OMN, the design procedure of the presented DB‐FPA is summarised. To verify the proposed idea, a novel DB‐FPA has been fabricated and measured. During the two frequency bands, the measured maximum power added efficiencies are 55.11% and 47.78% within 33.0 dBm input power, while the measured peak gains are 15.4 and 14.0 dB within 15.0 dBm input power.

Abstract In recent years, the behavior recognition technology based on Wi-Fi devices has been favored by many researchers. Existing Wi-Fi-based human behavior recognition technology mainly uses classification algorithms to construct classification models, which has problems such as inaccurate behavior segmentation, failure to extract deep-level features from the original data and design classification models matching the proposed features in the process of behavior recognition. In order to solve above problems, this paper proposes a window variance comparison method by combining the adaptive thresholds calculated to achieve effective segmentation of multiple discontinuous human behaviors, then uses the short-time Fourier algorithm to extract time-frequency features of individual behavior, and extracts the graph structure data from the autocorrelation matrix of time-frequency features and the features themselves. A graph neural network is built for behavior recognition. The experimental results show that the segmentation accuracy of the behavior segmentation method in the two scenes is 0.964 and 0.993, which is better than the existing threshold-based behavior segmentation methods. In addition, this paper extracts graph structure data by spectral energy change method and builds behavior recognition model by using graph neural network, and the recognition accuracy is significantly improved compared with the traditional classification algorithm.

IntroductionTicks are important blood-sucking ectoparasites that can transmit various pathogens, posing significant threats to the wellbeing of humans and livestock. Dabieshan tick virus (DBTV) was initially discovered in 2015 in Haemaphysalis longicornis ticks from the Dabieshan mountain region in Hubei Province, China. In recent years, DBTV has been discovered in various regions of China, including Shandong, Zhejiang, Liaoning, Hubei, Yunnan, and Guizhou Provinces. However, the researches on tick-borne transmission of DBTV are scarce.MethodsThis study utilized the small RNA sequencing (sRNA-seq) method to identify tick-associated viruses in ticks collected from Chengde in Hebei Province and Yongcheng in Henan Province, leading to the discovery of a new DBTV strain in Hebei. The complete coding genome of DBTV Hebei strain was obtained through RNA-seq and Sanger sequencing. Furthermore, the transmission experiment of DBTV in H. longicornis was examined in laboratory for the first time.ResultsDBTV was detected in newly molted adult H. longicornis ticks collected in Chengde, Hebei Province. Additionally, DBTV was also detected in both unfed nymphs and engorged females of H. longicornis collected from Chengde, with a positive rate of 20% and 56.25%, respectively. The complete coding genome of DBTV (OP682840 and OP716696) were obtained, and phylogenetic analysis revealed that the DBTV Hebei strain clustered with previously reported DBTV strains. Furthermore, this virus was observed in engorged females, eggs, and larvae of the subsequent generation.DiscussionIt is necessary to expand the scope of DBTV investigation, particularly in northern China. This study demonstrated that DBTV can be transmitted from engorged females to larvae of the next generation. Moreover, the detection of DBTV in unfed nymphs and adults (which moulted from engorged nymphs) collected from the filed of Chengde suggests that H. longicornis serves as a potential transmission host and reservoir for DBTV through transstadial and transovarial transmission. However, there remains a lack of research on the isolation and pathogenicity of DBTV, highlighting the need for further studies to mitigate potential harm to the health of animals and humans.

The failure of ultra-high performance concrete (UHPC) under axial compression has significant brittleness, so it is necessary to use fiber reinforced polymer (FRP) to constrain UHPC for achieving ductile failure. This article mainly summarized the research progress of FRP constrained UHPC columns, analyzed the influence of different variables on the compressive performance of FRP-confined UHPC columns and the coupling effect between variables, compared the axial compressive stress-strain models of FRP-confined UHPC columns proposed by previous scholars, and evaluated the existing models using a large amount of collected experimental data. The evaluation results indicated that the existing models provided relatively accurate predictions for ultimate stress, but further improvement and correction are needed for predicting ultimate strain. Based on the data collected in this study, the models had been modified, resulting in improved accuracy in predicting both ultimate stress and ultimate strain.

The composite flame retardant composed of aluminum hydroxide (ATH) and organic montmorillonite (OMMT) is of potential application value in the tunnel asphalt pavement. To investigate the effect of ATH and OMMT on the rheological properties of SBS modified asphalt at high and low temperatures. In this study, the composite ATH/OMMT/SBS modified asphalt was subjected to multiple stress creep recovery (MSCR), linear amplitude sweep test (LAS), and Bending beam rheometer (BBR) experiments. Analysis of high and low temperature rheological properties and fatigue properties of composite ATH/OMMT/SBS modified asphalt under the conditions of single incorporation and composite modification. The findings of the tests suggest that ATH can significantly improve the fatigue life but has adverse impact on the creep recovery property of asphalt materials, OMMT has a significant improvement effect on the low-temperature performance, however, the detrimental effect of OMMT on fatigue performance is greater than the improvement of ATH. The research results provide a reference for the practical application of flame retardants.

Substituting petroleum asphalt with bio-asphalt produced by biological waste recycling can mitigate the dependence on non-renewable resource oil. This study aimed to investigate the effectiveness of bio-asphalt as a substitute for petroleum asphalt by analyzing its rheological properties. Therefore, in this study, modified asphalt was prepared using styrene-butadiene-styrene copolymer (SBS), polyphosphoric acid (PPA), and bio-asphalt (BA) as modifiers, and the short-term aging test of the modified asphalt was carried out. Then the rheological properties of the modified asphalt were analyzed through the Temperature Scanning (TS) test, the Multiple Stress Creep Recovery (MSCR) test, and the Bending Beam Rheometer (BBR) test; finally, the Fourier Transform infrared spectroscopy (FTIR) test, Fluorescence Microscopy (FM) test to study the modification mechanism and microscopic characteristics of asphalt. The results show that adding BA improved the low-temperature rheological properties of modified asphalt while reducing the high-temperature rheological properties of PPA modified asphalt. The BA, SBS, and PPA combination exhibited excellent performance in high and low-temperature conditions. The short-term aging process enhances the high-temperature rutting resistance of modified asphalt but adversely affects its low-temperature performance. Fluorescence microscopy tests have demonstrated that BA improves the compatibility of SBS and PPA in asphalt. Meanwhile, FTIR has revealed that BA and SBS are physically mixed, and BA and PPA undergo a chemical reaction. This study suggests that BA could be a viable and sustainable alternative to petroleum asphalt, with promising performance characteristics when combined with SBS and PPA modifiers.

Asphalt pavements are inevitably affected by coupled effects of dry-wet/freeze-thaw cycles, UV radiation, temperature, and loading during service, resulting in degradation of their road performance and reduced service life. Therefore, this study used crumb rubber (CR) and polyphosphoric acid (PPA) as modifiers to prepare CR/PPA composite modified asphalt mixtures for testing. To investigate the mechanical properties of the composite modified asphalt mixtures under the action of multi-factor coupling. Meanwhile, the performance analysis of matrix asphalt and SBS modified asphalt mixtures were carried out as a comparison. Density and water penetration tests, splitting tests, beam bending tests, and four-point bending fatigue tests reveal the evolution of asphalt mixture road performance. Finally, regression fitting was performed on the test data to establish the mechanical damage model of each type of asphalt mixture. The results show that with the increase of wet-dry/freeze-thaw cycles and the increase of UV radiation time, the void ratio and water permeability coefficient increase and the splitting strength, bending and tensile strain, and fatigue life decrease for all types of asphalt mixtures. When the number of wet-dry/freeze-thaw cycles reached 8, the mechanical properties of the asphalt mixture decreased significantly, while the changes leveled off after the number of cycles reached 15. When the UV radiation time is less than 4 months, the mechanical properties of the asphalt mixture decay more slowly. However, with a further increase in radiation time, the mechanical properties decay rapidly. The water-saturated specimens have a more significant effect on the mechanical damage of each type of asphalt mixture. Compared with the other two mixtures, the composite modified asphalt mixtures have superior mechanical properties and fatigue life. The mixture performance damage model based on the least squares Levenberg-Marquardt method (L-M method) was able to simulate the experimental data well, with a minimum correlation coefficient R2 of 0.9285. In summary, the road performance of all types of asphalt mixtures under the coupling of multiple factors has deteriorated, while the composite modified asphalt mixtures have better anti-damage characteristics, with good prospects for promotion.

Abstract Aqueous iron batteries are appealing candidates for large-scale energy storage due to their safety and low-cost aspects. However, the development of aqueous Fe batteries is hindered by their inadequate long-term cycling stability. Here, we propose the synthesis and application as positive electrode active material of cross-linked polyaniline (C-PANI). We use melamine as the crosslinker to improve the electronical conductivity and electrochemical stability of the C-PANI. Indeed, when the C-PANI is tested in combination with a Fe metal negative electrode and 1 M iron trifluoromethanesulfonate (Fe(TOF)2) electrolyte solution, the coin cell can deliver a specific capacity of about 110 mAh g−1 and an average discharge voltage of 0.55 V after 39,000 cycles at 25 A g−1 with a test temperature of 28 °C ± 1 °C. Furthermore, mechanistic studies suggest that Fe2+ ions are bonded to TOF− anions to form positively charged complexes Fe(TOF)+, which are stored with protons in the C-PANI electrode structures. Finally, we also demonstrate the use of C-PANI in combination with a polymeric hydrogel electrolyte to produce a flexible reflective electrochromic lab-scale iron battery prototype.

Abstract Introduction In total hip arthroplasty (THA), the correct position of the acetabular component directly determines the outcome of the surgery, or the success of the surgery. Therefore, how to accurately locate the position of the acetabular component has become a very critical step in THA. As an important anatomical structure of the hip joint, the transverse acetabular ligament (TAL) is helpful for acetabular component orientation in THA. The aim of this systematic review was to investigate application of TAL in THA. Materials and methods A systematic literature search of PUBMED, EMBASE, and Cochrane Library was performed (January and February 2023) using keywords “total hip arthroplasty,” “total hip replacement,” “total hip replacements,” “total hip arthroplasties,” “total hip prosthesis,” and “transverse acetabular ligament” in all possible combinations. Reference lists of included articles were reviewed. Study design, surgical approach, patient demographics, TAL identification rate, appearance of the TAL, anteversion and inclination angle and rate of dislocations were recorded. Results In total, 19 studies met the screening criteria. Study designs were prospective cohorts (42%), retrospective cohorts (32%), Case series (21%), and randomized controlled trial (5%). Twelve of the 19 (63.2%) studies investigated the application of TAL as an anatomical landmark for locating acetabular component position in THA. Analysis revealed that TAL is a reliable anatomical landmark for acetabular component orientation within the safe zone in THA. Conclusions TAL can reliably be used to align the acetabular component in the safe zone for anteversion and inclination in THA. However, TAL has individual variation influenced by some risk factors. More randomized controlled studies with larger numbers of patients are needed to investigate the precision and accuracy of TAL as an intraoperative landmark in THA. Level of evidence IV.

To solve the problem that the quantum signal was disturbed by the scattering environment, which lead to large fluctuation of the positioning error and poor positioning robustness, an optical quantum positioning method based on random resampling of the scattering free path was proposed.The scattering model was established based on the Monte Carlo theory, and the scattering free path matrix was constructed by combining the environment information and coincidence counting results.The scattering free program was resampled, and the relative distance error of different light sources in different time groups was calculated.Based on that, the light sources were dynamically selected, and the position of the target object was estimated.Experimental results show that the positioning error of the proposed method can achieve the centimeter level, and the confidence probability of the positioning error within 0.12 m is 90.57%.

Nucleic acid sensing involving CRISPR technologies is powerful but has certain limitations, such as PAM sequence requirements and limited multiplexing. Here, authors report a CRISPR-based barcoding technology which enables multiple outputs from any target sequence, based on cis- and trans-cleavage.

The Industrial Metaverse provides unparalleled prospects for increasing productivity and efficiency across multiple sectors. As wireless sensor networks play an important role in data collection and transmission within this ecosystem, preserving context privacy becomes critical to protecting sensitive information. This paper investigates the issue of context privacy preservation for user validation via AccesSensor in the Industrial Metaverse and presents a technological method to address it. We explore the need for context privacy, look at existing privacy preservation solutions, and propose novel user validation methods that are customized to the Industrial Metaverse’s access system. This method is evaluated on time-based efficiency, privacy method and bandwidth utilization. Our method performs better as compared to the DPSensor. Our research seeks to provide insights and recommendations for developing strong privacy protection methods in wireless sensor networks that operate within the Industrial Metaverse ecosystem.

The recent expansion of shellfish mariculture could significantly impact the ocean carbon cycle and its associated biogeochemical processes. To understand the source and fate of particulate organic carbon (POC), a summer cruise was conducted from September 8 to 10, 2022, at a mussel farm on Gouqi Island and its adjacent areas located in the East China Sea. Parameters included in situ temperature and salinity, contents of dissolved oxygen (DO), suspended particulate matter (SPM), POC, and chlorophyll a (Chl a), as well as the stable carbon isotopic composition (δ13C) of organic matter in particle and sediment samples, which were analyzed to facilitate a comparative assessment of the areas inside and outside the mussel farm. The POM was much fresher (POC/Chl a < 150) inside the farm with little impact from sediment resuspension (lower SPM content, 11.6 ± 6.6 mg/L), while a significant influence of sediment resuspension was found outside the farm (SPM > 20 mg/L, POC/Chl a > 150). A two end-member mixing model showed that 82.0 ± 6.0% of POC originated from marine algae within the farm, much higher than that outside the farming area (66.1 ± 7.8%). Moreover, elevated DO saturation but relatively low Chl a concentration within the farm suggested continuous algae consumption following potential high productivity. The averaged δ13C values were similar among suspended POC, sinking POC, and sedimentary organic carbon within the farm, implying the fast export and burial of POC. This is likely due to the filter-feeding habits of mussels, who ingest fresh POC and then pack it as fecal pellets that rapidly settle into the sediment. This study sheds light on the distribution and sources of POM inside and outside the mussel farm on Gouqi Island, enhancing our understanding of the marine carbon cycle on shellfish farms and providing insights into the underlying biogeochemical processes.

A UAV-assisted space–air–ground integrated network (SAGIN) can provide communication services for remote areas and disaster-stricken regions. However, the increasing types and numbers of ground terminals (GTs) have led to the explosive growth of communication data volume, which is far from meeting the communication needs of ground users. We propose a mobile edge network model that consists of three tiers: satellites, UAVs, and GTs. In this model, UAVs and satellites deploy edge servers to deliver services to GTs. GTs with limited computing capabilities can upload computation tasks to UAVs or satellites for processing. Specifically, we optimize association control, bandwidth allocation, computation task allocation, caching decisions, and the UAV’s position to minimize task latency. However, the proposed joint optimization problem is complex, and it is difficult to solve. Hence, we utilize Block Coordinate Descent (BCD) and introduce auxiliary variables to decompose the original problem into different subproblems. These subproblems are then solved using the McCormick envelope theory, the Successive Convex Approximation (SCA) method, and convex optimization techniques. The simulation results extensively illustrate that the proposed solution dramatically decreases the overall latency when compared with alternative benchmark schemes.

The Hippo pathway plays crucial roles in governing various biological processes during tumorigenesis and metastasis. Within this pathway, upstream signaling stimuli activate a core kinase cascade, involving MST1/2 and LATS1/2, that subsequently phosphorylates and inhibits the transcriptional co-activators YAP and its paralog TAZ. This inhibition modulates the transcriptional regulation of downstream target genes, impacting cell proliferation, migration, and death. Despite the acknowledged significance of protein kinases in the Hippo pathway, the regulatory influence of protein phosphatases remains largely unexplored. In this study, we conducted the first gain-of-functional screen for protein tyrosine phosphatases (PTPs) regulating the Hippo pathway. Utilizing a LATS kinase biosensor (LATS-BS), a YAP/TAZ activity reporter (STBS-Luc), and a comprehensive PTP library, we identified numerous novel PTPs that play regulatory roles in the Hippo pathway. Subsequent experiments validated PTPN12, a master regulator of oncogenic receptor tyrosine kinases (RTKs), as a previously unrecognized negative regulator of the Hippo pathway effectors, oncogenic YAP/TAZ, influencing breast cancer cell proliferation and migration. In summary, our findings offer valuable insights into the roles of PTPs in the Hippo signaling pathway, significantly contributing to our understanding of breast cancer biology and potential therapeutic strategies.

The escalating impact of global warming on crop yield and quality poses a significant threat to future food supplies. Breeding heat-resistant crop varieties holds promise, but necessitates a deeper understanding of the molecular mechanisms underlying plant heat tolerance. Recent studies have shed light on the initial events of heat perception in plants. In this review, we provide a comprehensive summary of the recent progress made in unraveling the mechanisms of heat perception and response in plants. Calcium ion (Ca2+), hydrogen peroxide (H2O2), and nitric oxide (NO) have emerged as key participants in heat perception. Furthermore, we discuss the potential roles of the NAC transcription factor NTL3, thermo-tolerance 3.1 (TT3.1), and Target of temperature 3 (TOT3) as thermosensors associated with the plasma membrane. Additionally, we explore the involvement of cytoplasmic HISTONE DEACETYLASE 9 (HDA9), mRNA encoding the phytochrome-interacting factor 7 (PIF7), and chloroplasts in mediating heat perception. This review also highlights the role of intranuclear transcriptional condensates formed by phytochrome B (phyB), EARLY FLOWERING 3 (ELF3), and guanylate-binding protein (GBP)-like GTPase 3 (GBPL3) in heat perception. Finally, we raise the unresolved questions in the field of heat perception that require further investigation in the future.

Purpose – The purpose of this paper is to show that the droplet impact phenomenon is important for the advancement of industrial technologies in many fields such as spray cooling and ink jet printing. Droplet bouncing on the nonwetting surfaces is a special phenomenon in the impact process which has attracted lots of attention. Design/methodology/approach – In this work, the authors fabricated two kinds of representative nonwetting surfaces including superhydrophobic surfaces (SHS) and a slippery liquid-infused porous surface (SLIPS) with advanced UV laser processing. Findings – The droplet bouncing behavior on the two kinds of nonwetting surfaces were compared in the experiments. The results indicate that the increasing Weber number enlarges the maximum droplet spreading diameter and raises the droplet bounce height but has no effect on contact time. Originality/value – In addition, the authors find that the topological SHS and SLIPS with the laser-processed microwedge groove array produce asymmetric droplet bouncing with opposite offset direction. Microdroplets can be continuously transported without any additional driving force on such a topological SLIPS. The promising method for manipulating droplets has potential applications for the droplet-based microfluidic platforms.