Search

In this paper, a novel double-rotor hybrid excited axial switched-flux permanent magnet (DRHE-ASFPM) machine incorporating the advantages of the axial switched-flux permanent magnet (PM) machine and wound field synchronous machine is investigated. Similar to the switched-flux PM machine, there is a large undesired cogging torque in the DRHE-ASFPM machine because of high air-gap density and strong flux-focusing effect. This paper presents and compares three cogging torque reduction techniques in the DRHE-ASFPM machine, including stator/rotor teeth notching, stator slot chamfering, as well as stator slot chamfering and teeth notching (SCTN). The analytical expression of the cogging torque is derived and three methods are analyzed and compared in detail. As verified by 3-D finite element (FE) method, the SCTN technique can effectively reduce the cogging torque extremely, but slightly decrease the output torque under both PM and hybrid excitation modes. Finally, an optimized prototype machine with SCTN structure is manufactured, and the experiment is performed to validate the FE predicted results.

This paper proposes a novel axial flux rotor consequent-pole permanent magnet (AFRCPPM) machine with improved torque density. The key is to place the PMs between the adjacent rotor teeth with switched-flux feature compared to the stator permanent magnet (PM) dual-stator axial switched-flux PM (DS-ASFPM) machine. This will be able to obtain simple stator structure, high power density and large torque output capability. In the following paper, the topology, operating principle and winding configuration are analyzed in detail. According to the theoretical derivation, the major parameters of the AFRCPPM machine are acquired. Based on 3-D finite-element (FE) method, the electromagnetic performance of the AFRCPPM machine and DS-ASFPM machine is comparatively investigated to evaluate their characteristics. The FE-predicted results indicate that the AFRCPPM machine exhibits simple stator structure, sinusoidal back-EMF and high power/torque density. Moreover, under the same armature current, the AFRCPPM machine has larger output torque compared to the DS-ASFPM machine. Hence, it has a good prospect in electric vehicle (EV) or hybrid EV.

Background: PM2.5 exposure is associated with lung adenocarcinoma (LUAD), but the mechanism is unclear. The lack of understanding impedes our effort on prevention. This study examined a possible mechanism of lung cancer caused by PM2.5 exposure, and aimed to find a potential intervention for people living in PM2.5 polluted regions.Methods: PM2.5 was collected near South-North elevated way in Shanghai city. Lung adenocarcinoma cells, pulmonary epithelial cell and mice were exposed to PM2.5 or D-limonene for 48h and two month separately. Cell morphology, abilities of invasion, migration and proliferation, pulmonary fibrosis level were assessed. Lipid metabolism changes were also studied in 11,564 lung cancer patients.Results: PM2.5 exposure induced accumulation of lipid droplets in LUAD cells which was accompanied by increased malignant cellular behaviors and disrupted lipid metabolism. PM2.5 exposure of LUAD led to cleaved N-SREBP1 translocation from the cytoplasm to nucleus, which upregulated the expression of FASN and ACACA. These changes increased lipid accumulation in LUAD. D-limonene, a natural monoterpene, was found to inhibit the changes in lipid metabolism through upregulating the expression of miR-195, a reported tumor suppressor that inhibited the expression of SREBP1, FASN and ACACA specifically induced by PM2.5 exposure. Same changes were also observed in normal lung epithelial cells and normal lung tissue from mice after PM2.5 exposure, and the changes were inhibited by D-limonene treatment. Furthermore, in a cohort of 11,564 lung cancer patients, significant lipid metabolism disorders were observed in high, compared to low, PM2.5 polluted areas, and a small human intervention trial showed that serum miR-195 was upregulated after oral intake of D-limonene.Conclusion: Our study reveals a potential intervention target for LUAD that is related to PM2.5 exposure-induced lipid metabolism disturbance and that D-limonene may inhibit the disruption through the upregulation of miR-195, which suggests a novel low-cost preventive intervention for people living in PM2.5 polluted regions.

In this paper, a novel 6-stator-pole double-rotor hybrid excited axial switched-flux permanent magnet (DR-HEASFPM) machine is proposed for electrical vehicles. Based on 3-D finite element analysis, the influence of 10-, 11-, 13-, and 14-rotor-pole on the electromagnetic performance of DR-HEASFPM machine, including the magnetic field distribution, flux-linkage, back-EMF, flux regulation principles and capability, is comparatively investigated. The results show that the 6/10-, Ll-pole DR-HEASFPM machines exhibit higher PM flux-linkage and back-EMF. Moreover, the back-EMF of 6/11-pole machine is more sinusoidal and the flux-regulation capability is more excellent, which is suitable for wide speed-regulation application.

ATP/GTP binding protein like 1 (AGBL1) plays a role in controlling the length of polyglutamate side chains. Polymorphism rs4513061 in AGBL1 is suspected to influence the risk of lung cancer. A case...

Background: Lipid metabolism disorder, a new hallmark of cancer initiation, has been involved in lung adenocarcinoma (LUAD). However, few biomarkers about lipid metabolism-related genes (LMRGs) have been developed for prognosis prediction and clinical treatment of LUAD patients.Methods: In this study, we constructed and validated an effective prognostic prediction model for LUAD patients depending on LMRGs. Subsequently, we investigated the prediction model from immune microenvironment, genomic changes, and immunotherapy.Results: Then, eleven LMRGs were identified and applied to LUAD subtyping. In comparison with the high-risk group, the low-risk group exhibited a remarkably favorable prognosis, along with a higher immune score and lower tumor purity. Moreover, the low-risk group presented higher levels of immune checkpoint molecules, lower tumor immune dysfunction and exclusion (TIDE) score and tumor mutation burden (TMB), and higher likelihood of benefiting from immunotherapy. Furthermore, the genomic changes of six LMRGs (CD79A, HACD1, CYP17A1, SLCO1B3, ANGPTL4, and LDHA) were responsible for the difference in susceptibility to LUAD by greatly influencing B-cell activation.Conclusion: Generally speaking, the LMRG model is a reliable independent biomarker for predicting adverse outcomes in LUAD patients and has the potential to facilitate risk-stratified immunotherapy.

Lung cancer represents a complex and malignant cancer. Close Homologue of L1 (CHL1) gene plays a crucial role in the progress of cancer. The aim of this study is to explore the association between CHL1 rs425366 polymorphism and lung cancer susceptibility in northeast of China. A hospital-based case-control study was carried out to collect relative characteristics. Logistic regression analysis was conducted to analyze the relationship between single nucleotide polymorphisms and lung cancer susceptibility. The results suggested that there was statistically significant difference between GT genotype and TT genotype of rs425366 and lung cancer susceptibility. In stratified analysis, TT genotype of rs425366 may increase the risk of lung adenocarcinoma. We also found that non-smoking individuals carrying T allele were more likely to develop lung cancer. Overall, our study may indicate that CHL1 gene may increase lung cancer susceptibility in northeast of China.

// Xiaoying Li 1, 2 , XueLian Li 1, 2 , Yangwu Ren 1, 2 , Zhihua Yin 1, 2 , Xiaowei Quan 1, 2 , Xiaoxia Xue 3 and Baosen Zhou 1, 2 1 Department of Epidemiology, School of Public Health, China Medical University, Shenyang, China 2 Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Department of Education, Liaoning, China 3 The Third Center of Laboratory Technology and Experimental Medicine, China Medical University, Shenyang, China Correspondence to: Baosen Zhou, email: bszhou@cmu.edu.cn Keywords: single nucleotide polymorphisms; MIR217HG; Gab1; lung cancer; susceptibility Received: August 31, 2017 Accepted: September 23, 2017 Published: October 24, 2017 ABSTRACT Lung cancer is one of the malignant tumors with the highest morbidity and mortality all over the world. Here we researched the association between two SNPs (rs1347093 in MIR217HG and rs1397529 in Gab1) and the risk of lung cancer in northeast Chinese population, including 825 cases and 766 controls. We carried out χ 2 test, unconditional logistic regression analysis and crossover analysis to estimate the relationship between SNPs and lung cancer risk and the interaction between SNPs and smoking on susceptibility to lung cancer. The results indicated that rs1347093, rs1397529 polymorphisms were associated with lung cancer risk, especially with adenocarcinoma risk. Dominant genetic model of the rs1347093 was associated with reduced risk of lung cancer compared to CC genotype (AC+AA vs. CC: adjusted OR = 0.599, 95%CI = 0.418-0.858, P= 0.005). For rs1347093, the similar result was found. Dominant genetic model of the rs1397529 was associated with reduced risk of lung cancer compared to AA genotype (AC+CC vs. AA: adjusted OR = 0.664, 95%CI = 0.491-0.897, P= 0.008). There is no significant interaction between rs1347093, rs1397529 polymorphism and smoking on susceptibility to lung cancer. Our study might demonstrate that rs1347093 in MIR217HG and rs1397529 in Gab1 could be meaningful as the novel biomarker for lung cancer risk.

// Xue Fang 1, 2, 4 , Xuelian Li 1, 2 , Zhihua Yin 1, 2 , Lingzi Xia 1, 2 , Xiaowei Quan 1, 2 , Yuxia Zhao 3 and Baosen Zhou 1, 2 1 Department of Epidemiology, School of Public Health, China Medical University, Shenyang, China 2 Liaoning Provincial Department of Education, Key Laboratory of Cancer Etiology and Prevention, China Medical University, Liaoning, China 3 Department of Radiotherapy, The Fourth Affiliated Hospital of China Medical University, Shenyang, China 4 Department of Epidemiology, School of Public Health, Shenyang Medical College, Shenyang, China Correspondence to: Baosen Zhou, email: bszhou@mail.cmu.edu.cn Keywords: single nucleotide polymorphism, lung cancer, CAV1, microRNA Received: April 27, 2017 Accepted: September 05, 2017 Published: October 09, 2017 ABSTRACT Single nucleotide polymorphism (SNP) may influence the genesis and development of cancer in a variety of ways depending on their location. Here we conducted a study in Chinese female non-smokers to investigate the relationship between rs1049337, rs926198 and the risk or survival of lung cancer. Further, we explored whether rs1049337 could alter the binding affinity between the mRNA of CAV1 and the corresponding microRNAs. Finally, we evaluated the relationship between expression level of CAV1 and prognosis of lung cancer. The results showed that the rs1049337-C allele and rs926198-C allele were the protective alleles of lung cancer risk. Haplotype analysis indicated that the C-C haplotype (constructed by rs1049337 and rs926198) was a protective haplotype for lung cancer risk. The result of luciferase reporter assay showed that rs1049337 can affect the binding affinity of CAV1 mRNA to the corresponding microRNAs both in A549 cell line and H1299 cell line. Compared with C allele, T allele had a relatively decreased luciferase activity. Compared with paired normal adjacent tissue or normal lung tissue, lung cancer tissue showed a relatively low level of CAV1. Refer to those patients at early stage of lung cancer, the expression level of CAV1 in patients at late stage of lung cancer was relatively low. In conclusion, the results indicated that rs1049337, it’s a SNP located at 3’UTR region of CAV1 may affect lung cancer risk by altering the binding affinity between the mRNA of CAV1 and the corresponding microRNAs.

// Xu Feng 1, 2 , Xue Fang 1, 3 , Lingzi Xia 1, 3 , Yangwu Ren 1, 3 , Xuelian Li 1, 3 , Xiaowei Quan 1, 3 and Baosen Zhou 1, 3 1 Department of Epidemiology, School of Public Health, China Medical University, Liaoning, China 2 Department of Health Statistics, School of Public Health, Shenyang Medical College, Liaoning, China 3 Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Department of Education, Liaoning, China Correspondence to: Baosen Zhou, email: bszhou@mail.cmu.edu.cn Keywords: PPP1R13L, CD3EAP, single nucleotide polymorphism, non-small cell lung cancer Received: April 11, 2017 Accepted: July 19, 2017 Published: August 12, 2017 ABSTRACT PPP1R13L and CD3EAP were confirmed to play important roles in transcription and apoptosis. SNPs in PPP1R13L and CD3EAP may be associated with lung cancer risk and survival. This study investigated the association of PPP1R13L rs1005165 and CD3EAP rs967591 with non-small cell lung cancer (NSCLC) risk and survival in Chinese non-smoking females. 442 NSCLC cases and 480 cancer-free controls were conducted in the case-control study, and 283 cases were in cohort study. Genotype was determined by Taqman real-time PCR. The statistical analyses were performed by SPSS 22.0 software. We found that rs1005165 and rs967591 were significantly associated with NSCLC risk in Chinese non-smoking females. For rs1005165, compared with homozygous wild CC genotype, carriers of CT or TT genotype had lower risk of NSCLC (adjusted ORs were 0.675 and 0.713, 95% CI were 0.461-0.988 and 0.525-0.968, respectively), adjusted OR for dominant model was 0.702, 95% CI was 0.526-0.937. For rs967591, AA genotype (adjusted OR = 0.721, 95% CI = 0.532-0.978) and at least one A allele (GA+AA) (adjusted OR = 0.716, 95% CI = 0.536-0.956) were significantly correlated with lower risk of NSCLC, compared with GG genotype. But we didn’t find correlation between the two SNPs and survival time in Chinese non-smoking NSCLC females. In general, we found PPP1R13L rs1005165 and CD3EAP rs967591 might be associated with lower NSCLC risk in Chinese non-smoking females, but no significant relationship was found with NSCLC survival.

ATP/GTP binding protein like 1 (

The connection between cancer and circadian rhythms has garnered recent attention.

Purpose: This study tried to explore whether members of miR-92a family contribute to early diagnosis and prognosis for human cancers and how they work. Methods: Integrated meta-analysis retrieved from public repositories was employed to assess the clinical roles of the miR-92a family for cancer diagnosis and prognosis. Expression level of miR-92a was detected by the TCGA database and was confirmed by non-small-cell lung cancer (NSCLC) tissues. Targets of miR-92a were predicted using starbase, and validated by dual luciferase assay. Correlation between miR-92a and the target gene was assessed by linkedOmics while expression of the target gene and its role in cancer prognosis were analyzed with UALCAN and Gepia. Results: We recognized the miR-92a family could serve as a potential diagnostic biomarker with a pooled sensitivity of 0.85 [0.81-0.88] and specificity of 0.86 [0.83-0.90]. The overall hazard ratio (HR) was 2.26 [95% CI: 1.70-3.00] for high expression groups compared to low expression groups. Expression of miR-92a was identified to be upregulated in NSCLC, especially in lung squamous cell carcinoma (LUSC). Results from starbase and dual luciferase assay indicated the regulator of G-protein signaling 3 (RGS3) was a direct target of miR-92a. Statistical negative correlation was found for the expression of miR-92a and RGS3. In addition, expression of RGS3 was downregulated in NSCLC and patients with the high expression had a poor prognosis (HR = 1.3) for LUSC patients. However, results were to the contrary for lung adenocarcinoma (HR = 0.7). Conclusion: This study revealed that miR-92a family could be ideal biomarkers for cancer diagnosis and prognosis, which might function through targeting RGS3.

Axial field switched-flux permanent magnet (PM) (AFSFPM) machine is a stator-PM axial-flux machine, which exhibits many merits including simple and robust structure, short axial length, and high power/torque density. In this paper, a kind of direct torque control (DTC) method with flux-weakening strategy implementation, for the AFSFPM machine, is investigated. According to the stage control schemes, a given method with self-adaption for the flux-linkage, by remaining direct-axis is zero, is achieved in constant toque region. A flux-weakening control strategy is implemented in constant power region. On the basis of the DTC method, the performance of the drive system in the entire operating region is investigated. The results indicate that the proposed control strategy reduces the direct-axis component of armature current while increases the efficiency. Moreover, the constant power operating range is broaden. The effectiveness of the proposed method and the characteristics of the AFSFPM machine are verified. Therefore, the machine is very interesting in electrical vehicles (EV) or hybrid EVs (HEVs) application.

Lung cancer is known to cause high mortality and morbidity. The study aimed to explore the association between rs3733845 and rs3733846 polymorphisms in the promoter region of miR-143/145 and the risk of lung cancer among 575 nonsmoking cases and 575 cancer-free controls in a Chinese female population. We genotyped two single nucleotide polymorphisms (SNPs) in the promoter region of miR-143/145 in 575 cases and 575 controls using TaqMan allelic discrimination method. Logistic regression analysis was conducted to assess the association between polymorphisms in the promoter of miR-143/miR-145 and risk of lung cancer females. Crossover analysis was used to explore the interaction between the two SNPs and environmental risk factors (cooking oil fume exposure and passive smoking exposure). The results showed that both rs3733845 and rs3733846 polymorphisms were associated with an increased lung adenocarcinoma risk in dominant model (adjusted odds ratio [OR] = 1.329, 95% confidence intervals [CIs] = 1.026-1.723

// Xue Fang 1, 2 , Zhihua Yin 1, 2 , Xuelian Li 1, 2 , Lingzi Xia 1, 2 , Xiaowei Quan 1, 2 , Yuxia Zhao 3 , Baosen Zhou 1, 2 1 Department of Epidemiology, School of Public Health, China Medical University, Shenyang, China 2 Key Laboratory of Cancer Etiology and Prevention, China Medical University, Liaoning Provincial Department of Education, Liaoning, China 3 Department of Radiotherapy, The Fourth Affiliated Hospital of China Medical University, Shenyang, China Correspondence to: Baosen Zhou, email: bszhou@mail.cmu.edu.cn Keywords: differentially expressed genes, functional single nucleotide polymorphism, non-small cell lung cancer, risk, survival Received: December 02, 2016 Accepted: January 11, 2017 Published: January 27, 2017 ABSTRACT DNA genotype can affect gene expression, and gene expression can influence the onset and progression of diseases. Here we conducted a comprehensive study, we integrated analysis of gene expression profile and single nucleotide polymorphism (SNP) microarray data in order to scan out the critical genetic changes that participate in the onset and development of non-small cell lung cancer (NSCLC). Gene expression profile datasets were downloaded from the GEO database. Firstly, differentially expressed genes (DEGs) between NSCLC samples and adjacent normal samples were identified. Next, by STRING database, protein-protein interaction (PPI) network was constructed. At the same time, hub genes in PPI network were identified. Then, some functional SNPs in hub genes that may affect gene expression have been annotated. Finally, we carried a study to explore the relationship between functional SNPs and NSCLC risk and overall survival in Chinese female non-smokers. A total of 488 DEGs were identified in our study. There are 29 proteins with a higher degree of connectivity in the PPI network, including FOS , IL6 and MMP9 . By using database annotation, we got 8 candidate functional SNPs that may affect the expression level of hub proteins. In the case-control study, we found that rs4754-T allele, rs959173-C allele and rs2239144-G allele were the protective allele of NSCLC risk. In dominant model, rs4754-CT+TT genotype were associated with a shorter survival time. In general, our study provides a novel research direction in the field of multi-omic data integration, and helps us find some critical genetic changes in disease.

Hybrid excited axial field flux-switching permanent magnet (AFFSPM) (HEAFFSPM) machine is a novel stator-exciting hybrid excitation machine, which combines the advantages AFFSPM machine and wound-field excited machine. In this paper, an improved model predictive torque control (MPTC) method with duty cycle control by optimizing the active voltage vector duration is proposed for the HEAFFSPM machine drive system to reduce torque and flux ripples. On the basis of the theoretical analysis, a discrete-time model of the machine is established. Considering the flux-enhancing and flux-weakening algorithms, the different multiobjective cost functions are designed, and the multiple right weight coefficients are analyzed and chosen to optimize the system performance. The results indicate that the proposed control methods reduce the torque and flux ripples significantly compared with traditional MPTC, and the control system has better steady performance. Meanwhile, the load capacity is increased and the range of speed regulation is broadened.

Objective: The clinical use of intermittent infusion of vancomycin (IIV) and continuous infusion of vancomycin (CIV) is controversial. The aim of this study was to assess the effectiveness and safety of IIV and CIV by using a meta-analysis for cohort studies and randomized controlled trials. Methods: We compared the probabilities of target attainment (PTA) for the measured concentration (Cm) ≥the target concentration (Ct), the PTA for the area under the drug concentration curve/minimal inhibitory concentration (AUC/MIC) ≥400, the duration of treatment, nephrotoxicity, and overall mortality after vancomycin treatment as reported in PubMed, Embase, Cochrane, and Web of Science. Results: A total of 14 studies with 1640 patients were included in the meta-analysis. For IIV, the PTA of Cm ≥ Ct (RR = 0.72, 95% CI = 0.60–0.88), and nephrotoxicity (RR = 1.70, 95% CI = 1.34–2.14) were significantly different from those of CIV. The treatment duration (SMD = 0.08, 95% CI = −0.08–0.25), the PTA of AUC/MIC ≥ 400 (RR = 0.84, 95% CI = 0.70–1.00) and mortality (RR = 0.94, 95% CI = 0.72–1.25) were not significantly different from those of CIV. Conclusions: The results showed that CIV was easier to achieve Ct and safer than IIV. Additional randomized controlled trials focusing on the concentration of vancomycin are needed for further analysis. Keywords: Vancomycin, Intermittent, Continuous, Effectiveness, Safety

Lung cancer is the principal cause of cancer-associated deaths. HMGB1 has been reported to be associated with tumorigenesis. This study aimed to investigate the relationship between rs1412125 and rs1360485 polymorphisms in HMGB1 and the risk and survival of lung cancer. 850 cases and 733 controls were included. Logistic regression analysis and survival analysis were performed to investigate the association between SNPs and the risk and survival of lung cancer. Crossover analysis was used to analyze the interaction between SNPs and tobacco exposure. Results indicated that rs1412125 polymorphism was associated with lung cancer risk, especially with the risk of lung adenocarcinoma and small cell lung cancer. Carriers with CT and CC genotypes had a decreased risk of lung cancer (CT + CC vs.TT: adjusted OR = 0.736, p = 0.004). Similar results were obtained in the stratification analysis for non-smokers and female population. For rs1360485 polymorphism, AG and GG genotypes could decrease the risk of lung adenocarcinoma and female lung cancer by 0.771-fold and 0.789-fold. However, no significant interaction between polymorphisms and tobacco exposure or association between SNPs and the survival of lung cancer was observed. This study indicated polymorphisms in HMGB1 may be a novel biomarker for female lung adenocarcinoma risk.

(1) Background. Non-small cell lung cancer (NSCLC) has a high mortality rate. MiRNAs have been found to be diagnostic biomarkers for NSCLC. However, controversial results exist. We conducted this meta-analysis to evaluate the diagnostic value of miRNAs for NSCLC.(2) Methods. Databases and reference lists were searched. Pooled sensitivity (SEN), specificity (SPE), and area under the curve (AUC) were applied to examine the general diagnostic efficacy, and subgroup analysis was also performed.(3) Results. Pooled SEN, SPE, and AUC were 85%, 88%, and 0.93, respectively, for 71 studies. Multiple miRNAs (AUC: 0.96) obtained higher diagnostic value than single miRNA (AUC: 0.86), and the same result was found for Caucasian population (AUC: 0.97) when compared with Asian (AUC: 0.91) and Caucasian/African population (AUC: 0.92). MiRNA had higher diagnostic efficacy when participants contained both smokers and nonsmokers (AUC is 0.95 for imbalanced group and 0.91 for balanced group) than when containing only smokers (AUC: 0.90). Meanwhile, AUC was 0.91 for both miR-21 and miR-210.(4) Conclusions. Multiple miRNAs such as miR-21 and miR-210 could be used as diagnostic tools for NSCLC, especially for the Caucasian and nonsmoking NSCLC.

// Chang Zheng 1, 2 , Xuelian Li 1, 2 , Lingzi Xia 1, 2 , Xue Fang 1, 2 , Xiaowei Quan 1, 2 , Zhihua Yin 1, 2 , Yuxia Zhao 3 and Baosen Zhou 1, 2 1 Department of Epidemiology, School of Public Health, China Medical University, Shenyang, China 2 Key Laboratory of Cancer Etiology and Prevention, China Medical University, Liaoning Provincial Department of Education, Liaoning, China 3 Department of Radiotherapy, The Fourth Affiliated Hospital of China Medical University, Shenyang, China Correspondence to: Baosen Zhou, email: bszhou@mail.cmu.edu.cn Keywords: SNP, miRNA, NSCLC, susceptibility, prognosis Received: January 17, 2017 Accepted: March 21, 2017 Published: April 11, 2017 ABSTRACT Occurrence and development of non-small cell lung cancer (NSCLC) is a complex process affected both by gene and environment. Single nucleotide polymorphisms (SNPs) in microRNAs’ (miRNAs) biogenesis influenced the expression of mature miRNAs, further had an impact on risk of NSCLC. Our study focused on the correlation between rs213210, rs421446 or rs107822 polymorphisms in pri-miR-219-1 and susceptibility or prognosis of NSCLC in Chinese. A case-control study of 405 new-diagnosis patients and 405 controls was performed. Ten ml venous blood from each subject was collected for genotype test via using TaqMan allelic discrimination methodology and SPSS was performed for statistical analyses. We found that CC genotype in rs213210 (OR=3.462, 95%CI=2.222-5.394, P

// Xiaowei Quan 1, 2 , Zhihua Yin 1, 2 , Xue Fang 1, 2 , Baosen Zhou 1, 2 1 Department of Epidemiology, School of Public Health, China Medical University, Shenyang, China 2 Key Laboratory of Cancer Etiology and Prevention, China Medical University, Liaoning Provincial Department of Education, Liaoning, China Correspondence to: Baosen Zhou, email: bszhou@cmu.edu.cn Keywords: Notch1, single nucleotide polymorphisms, lung cancer, small cell lung cancer, non-smoking females Received: October 19, 2016 Accepted: March 01, 2017 Published: March 10, 2017 ABSTRACT Lung cancer is one of the most common cancers and the main cause of cancer-related deaths. Notch1 might play a part in tumorigenesis of lung cancer. Here we explored the relationship of three SNPs (rs3124599, rs3124607 and rs3124594) in Notch1 with the risk and the survival of lung cancer in non-smoking females, including 556 cases and 395 controls. Chi-square tests, logistic regression analysis and crossover analysis were conducted to estimate the association between SNPs and the risk of lung cancer and the interaction between SNPs and environmental exposure. Survival analysis was conducted to explore the association between SNPs and survival of lung cancer. The results demonstrated that the polymorphism of rs3124599 was associated with the susceptibility of lung cancer in recessive model (AA+AG vs. GG). Compared to the those with AA or AG genotype, individuals with GG genotype had a 1.562-fold increased risk of lung cancer ( P = 0.023, OR = 1.562, 95% CI = 1.062-2.297). In stratified analysis, the GG genotype of rs3124599 would increase the risk of small cell lung cancer (SCLC) ( P = 0.011, OR = 2.167, 95% CI = 1.193-3.396). However, no significant interaction between rs3124599 and cooking oil fume exposure was observed either in addictive model or multiplicative model. The results of survival analysis showed there was no significant association between SNPs and prognosis of lung cancer ( P = 0.949 for rs3124599, P = 0.508 for rs3124607, P = 0.884 for rs3124594). Our study might indicate that rs312599 in Notch1 may be a novel biomarker for SCLC risk in Chinese non-smoking females