27 results on '"Xiaoxiao Tao"'
Search Results
2. PREDICTION OF POST-STROKE DEPRESSION WITH COMBINED BLOOD BIOMARKERS IL-6, TNF-α, AND FATTY ACID BINDING PROTEIN: A PROSPECTIVE STUDY.
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Linlin Wang, Chunyou Chen, Jingang Zhu, Xianjun Bao, and Xiaoxiao Tao
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CARRIER proteins ,MENTAL depression ,HAMILTON Depression Inventory ,INTERLEUKIN-6 ,TUMOR necrosis factors ,FATTY acids - Abstract
Background: To investigate the expression levels of blood biomarkers interleukin-6 (IL-6), tumour necrosis factor (TNF-α), and intestinal fatty acid binding protein (iFABP) in patients with post-stroke depression (PSD), and their correlation with PSD occurrence. Methods: Clinical data of stroke patients admitted to the First People's Hospital of Wenling from December 2017 to December 2022 were retrospectively analyzed. Patients were classified into two groups based on their Hamilton Depression Rating Scale (HAMD) scores: PSD and non- PSD groups. The blood levels of IL-6, TNF-α, and iFABP were compared between the two groups, and their association with PSD occurrence was analyzed. Results: The PSD group had significantly higher levels of IL-6, TNF-α, and iFABP. The combined detection of these biomarkers demonstrated a greater predictive value for PSD occurrence compared to the individual detection of each biomarker. Conclusions: The study indicates that the levels of IL-6, TNF-α, and iFABP in the blood are significantly increased in patients with PSD. The combined detection of these biomarkers can effectively predict the occurrence of PSD, indicating high clinical value. Urod: Cilj je bio da se istraži ekspresija biomarkera u krvi interleukina-6 (IL-6), faktora tumorske nekroze (TNF-a) i proteina koji vezuje masne kiseline creva (iFABP) kod pacijenata sa depresijom nakon moždanog udara (PSD) i njihovim korelacija sa pojavom PSD-a. Metode: Retrospektivno su analizirani klinički podaci pacijenata sa moždanim udarom koji su primljeni u Prvu narodnu bolnicu Vbnling od decembra 2017. do decembra 2022. godine. Pacijenti su klasifikovani u dve grupe na osnovu rezultata Hamiltonove skale za procenu depresije (HAMD): PSD i ne-PSD grupe. Nivoi IL-6, TNF-a i iFABP u krvi su upoređeni između dve grupe i analizirana je njihova povezanost sa pojavom PSD. Rezultati: PSD grupa je imala značajno više nivoe IL-6, TNF- a i iFABP Kombinovana detekcija ovih biomarkera pokazala je veću prediktivnu vrednostza pojavu PSD-a u poređenju sa pojedinačnom detekcijom svakog biomarkera. Zaključak: Studija ukazuje da su nivoi IL-6, TNF-a i iFABP u krvi značajno povećani kod pacijenata sa PSD. Kombinovana detekcija ovih biomarkera može efikasno predvideti pojavu PSD-a, što ukazuje na visoku kliničku vrednost. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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3. Early selection efficiency for fiber dimensions and their relationships with growth and wood quality for Pinus elliottii Engelm. in southern China
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Jing Wen, Min Yi, Leiming Dong, Lu Zhang, Siyu Liu, Shenggui Yuan, Xiaoxiao Tao, and Meng Lai
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Forestry - Published
- 2023
4. A Preliminary Study on the Development and Application of Digital Twin Landscape Architectures in the Context of Smart City
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Shuzhen Zou, Xiaoxiao Tao, Biaohong Tao, and Guoxu Wu
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- 2022
5. Predictive Analysis of Serum NO, PGI2, and Ox-LDL Levels on Disease Progression in Patients with Lacunar Cerebral Infarction
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Xiaoxiao Tao, Chenfeng Qiu, Xuewen Feng, and Linlin Wang
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General Immunology and Microbiology ,Applied Mathematics ,Endothelial Cells ,General Medicine ,Cerebral Infarction ,Nitric Oxide ,Epoprostenol ,General Biochemistry, Genetics and Molecular Biology ,Lipoproteins, LDL ,Modeling and Simulation ,Stroke, Lacunar ,cardiovascular system ,Disease Progression ,Humans ,lipids (amino acids, peptides, and proteins) ,cardiovascular diseases - Abstract
Objective. To analyze and predict the progress of patients with lacunar infarction by analyzing the levels of serum NO, PGI2, and Ox-LDL produced by endothelial cells. Methods. 138 patients with lacunar infarction and 34 healthy people were selected. The selected samples were divided into progressive group, nonprogressive group, and control group for biochemical test and endothelial function test. The levels of serum NO, PGI2, and Ox-LDL were obtained. The observation indexes of different groups were compared for statistical analysis and multivariate logistic regression analysis. Results. The indexes of LI patients in the nonprogression group were different from those in the control group. The content of Ox-LDL in the nonprogression group was higher than that in the control group, while the indexes of serum NO and PGI2 were lower than that in the control group. The level of Ox-LDL in LI patients in the progressive group was much higher than that in healthy people in the control group seven days after admission, while the levels of serum NO and PGI2 were lower, and the difference of serum on was more obvious. The level of Ox-LDL in the progressive group was much higher than that in the nonprogressive group, while the levels of serum NO and PGI2 in the progressive group were lower, and the level of serum NO was significantly different from that in the nonprogressive group. Ox − LDL > 76.48 U / L , NO > 55.24 ummol / L , and PGI 2 > 29.78 ng / L were independent risk factors for the progression of lacunar infarction. Conclusion. Because the patients with lacunar infarction have endothelium-dependent relaxation disorder, the changes of serum NO, PGI2, and Ox-LDL can be used as the evaluation index of the disease progress of patients with lacunar infarction and can be widely used in clinical detection.
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- 2022
6. Protective Effect of Paeoniflorin on Acute Cerebral Infarction in Rats
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Zhengjun Chen, Chenfeng Qiu, Qian Zhu, Xiaomin Ma, Jinwei Yang, Xuewen Feng, Xiaoxiao Tao, Xianjun Bao, and Weilin Wu
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Male ,medicine.medical_specialty ,Ischemia ,Pharmaceutical Science ,02 engineering and technology ,030226 pharmacology & pharmacy ,Rats, Sprague-Dawley ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Glucosides ,Malondialdehyde ,Lactate dehydrogenase ,Internal medicine ,medicine ,Animals ,Nimodipine ,Neurons ,Superoxide Dismutase ,Cerebral infarction ,business.industry ,Brain ,Cerebral Infarction ,021001 nanoscience & nanotechnology ,medicine.disease ,Paeoniflorin ,Rats ,Disease Models, Animal ,Neuroprotective Agents ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Cerebral cortex ,Reperfusion Injury ,Acute Disease ,Monoterpenes ,Lipid Peroxidation ,Sodium-Potassium-Exchanging ATPase ,0210 nano-technology ,business ,Biotechnology ,medicine.drug - Abstract
Objective: The purpose of this paper was to study the protective effect of paeoniflorin on acute cerebral ischemia. The animal model of cerebral infarction induced by Middle Cerebral Artery Occlusion (MCAO) was blocked by the suture method. Sixty SD rats were randomly divided into the shame group, MCAO group, paeoniflorin (60, 120, 240 mg/kg, respectively) and Nimodipine (NMDP) group (n = 10 per group). Methods: The rats were intragastrically administered immediately after the operation. After 7 days of gavage, the brains were decapitated at 24 h. Hematoxylin and Eosin (HE) staining was used to observe the degree of cell damage in the cerebral cortex of rats. Immunohistochemistry was used to detect silver plating and to observe changes in nerve cells. Rats in the model group showed obvious symptoms of neurological deficits, such as the ischemic morphological changed, the Malondialdehyde (MDA), Lactate Dehydrogenase (LD) content and lactate dehydrogenase (LDH) activity were significantly increased in the ischemic brain tissue, while the Superoxide Dismutase (SOD) activity was decreased. Results: The decrease in Na+-K+-ATPase activity was significantly lower than that in the sham group. The neurological symptoms and signs of MCAO in the different doses of paeoniflorin group were improved, and the neuronal edema in the cortical area was alleviated. The activities of SOD, LDH and Na+-K+-ATPase were significantly increased, and the contents of MDA and LD were decreased. Conclusion: Therefore, paeoniflorin could alleviate the degree of tissue damage in rats with acute cerebral infarction, inhabit the formation of free radicals in the brain tissue after ischemia, and reduce the degree of lipid peroxidation. Thus, the degree of cell damage was reduced greatly and a protective effect was showed on cerebral ischemia.
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- 2020
7. Long Non-Coding RNA MEG3 Promotes Apoptosis of Vascular Cells and is Associated with Poor Prognosis in Ischemic Stroke
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Yi Zhang, Meiping Wang, Xiaoxiao Tao, Chenghua Xu, Wenjuan Chen, and Yu Geng
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Oncology ,Male ,medicine.medical_specialty ,China ,Apoptosis ,030204 cardiovascular system & hematology ,Severity of Illness Index ,03 medical and health sciences ,0302 clinical medicine ,In vivo ,Modified Rankin Scale ,Predictive Value of Tests ,Internal medicine ,Gene expression ,Internal Medicine ,medicine ,Animals ,Humans ,Maternally expressed gene 3 ,Aged ,MEG3 ,Ischemic stroke ,business.industry ,Gene Expression Profiling ,Biochemistry (medical) ,RNA ,Human brain ,Prognosis ,Long non-coding RNA ,Disease Models, Animal ,medicine.anatomical_structure ,Functional Status ,Gene Expression Regulation ,Case-Control Studies ,Original Article ,Female ,RNA, Long Noncoding ,Cardiology and Cardiovascular Medicine ,business ,Apoptosis Regulatory Proteins ,030217 neurology & neurosurgery - Abstract
Aim: This study focused on the expression pattern of long non-coding RNA maternally expressed gene 3 (MEG3) and its value in ischemic stroke (IS). Methods: The expression pattern and the roles of MEG3 in the development of IS were explored in mice IS model and human brain microvascular endothelial cells (hBMECs). A case-control study, including 215 IS patients and 153 controls, was also conducted to investigate its prognostic value. Results: In vivo study showed that MEG3 increased significantly in the IS group (P = 0.004), and its level remained stable within 3 to 48h after the onset of IS. Besides, the survival time of the mouse in the high MEG3 group was significantly lower than that in the low MEG3 group (P = 0.042). In vitro study showed that oxygen–glucose deprivation (OGD) treatment significantly up-regulated expressions of MEG3, Bax, and cleaved caspase-3, and further promoted apoptosis of hBMECs, while si-MEG3 blocked these effects. A human study showed that MEG3 increased markedly within 48h of IS onset and was positively associated with the National Institutes of Health Stroke Scale (r = 0.347, P < 0.001), modified Rankin Scale (r = 0.385, P < 0.001), high-sensitivity C-reactive protein (r = 0.221, P = 0.002) level, and infarct volume (r = 0.201, P = 0.006). Overall survival analysis showed that patients with higher MEG3 expression within 48h had a relatively poor prognosis (P < 0.001). Meanwhile, multivariate analysis revealed that MEG3 was an independent prognostic marker for unfavorable functional outcome and death in IS patients. Conclusions: This study suggested that MEG3 might be considered as an intervention point and potential prognostic indicator for IS.
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- 2020
8. Dihydromyricetin ameliorates oxygen‑glucose deprivation and re‑oxygenation‑induced injury in HT22 cells by activating the Wnt/β‑catenin signaling pathway
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Xiaoxiao Tao, Yaping Jiang, Xian Zheng, Xiaoxiao Ji, and Feifei Peng
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Oxygen ,Cancer Research ,Oxidative Stress ,Glucose ,Oncology ,Flavonols ,Cell Survival ,Genetics ,Molecular Medicine ,Apoptosis ,Molecular Biology ,Biochemistry ,Wnt Signaling Pathway - Abstract
Dihydromyricetin (DMY) is a natural flavonoid that possesses a wide range of pharmacological properties. The aim of the present study was to determine whether DMY could protect against nerve cell injury following ischemic stroke through antioxidant and neuroprotective effects. The effects of DMY on the viability, oxidative stress and apoptosis of HT22 cells following oxygen‑glucose deprivation and re‑oxygenation (OGD/R) were examined using MTT, lactate dehydrogenase (LDH), superoxide (SOD), malondialdehyde (MDA), western blot and TUNEL assays. Furthermore, Wnt/β‑catenin signaling proteins in OGD/R‑stimulated HT22 cells were detected in the presence or absence of DMY. In a separate experiment, the effect of DMY on OGD/R‑induced HT22 cell injury was also observed in the presence of the Wnt/β‑catenin inhibitor, XAV939. The results demonstrated that DMY had no impact on the survival of untreated HT22 cells, although DMY treatment significantly increased cell viability and inhibited cytotoxicity, oxidative stress and apoptosis following OGD/R. In addition, DMY upregulated the expression of Wnt/β‑catenin in OGD/R‑stimulated HT22 cells. In conclusion, DMY protected HT22 cells from OGD/R‑induced oxidative stress and apoptosis, and its effects may be mediated by the activation of the Wnt/β‑catenin signaling pathway.
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- 2021
9. Crosstalk between H1975 tumor cells and platelets to induce the proliferation, migration and tube formation of vascular endothelial cells
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Daiyin Peng, Xingyu Dong, Ting Zhu, Qinglin Li, Xiaoxiao Tao, Jimin Zhu, and Baikun Li
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Tube formation ,platelet ,Cancer Research ,medicine.diagnostic_test ,Cell growth ,Angiogenesis ,vascular endothelial cells ,Articles ,co-culture ,Umbilical vein ,Flow cytometry ,Cell biology ,Vascular endothelial growth factor ,chemistry.chemical_compound ,angiogenesis ,Oncology ,chemistry ,medicine ,cardiovascular system ,Platelet activation ,Wound healing ,lung carcinoma - Abstract
Activated platelets (PLTs) participate in the regulation of tumor angiogenesis, and tumors can activate PLTs. Whether co-culture of lung carcinoma with PLTs improves the function of human umbilical vein endothelial cells (HUVECs) requires further investigation. The present study aimed to investigate the impact of H1975 cell crosstalk with PLTs on the proliferation, migration and tube formation of HUVECs. Following generation of cell-derived supernatants and construction of the co-culture system, Cell Counting Kit-8, flow cytometry, transmission electron microscopy and a meter for epithelial measurement were performed to detect the proliferative ability of HUVECs. Furthermore, the wound healing and Transwell migration assays were performed to detect the migratory ability of HUVECs. A tube formation assay was performed to assess angiogenesis, ELISA was applied to detect the content of vascular endothelial growth factor (VEGF) and western blotting was carried out to measure the expression levels of VEGF receptor 2 (VEGFR2) in HUVECs. Compared with single-cultured HUVECs (control), co-culture with H1975 cells and PLTs (Exp_HP) improved cell proliferation, increased the proportion of cells in the S-phase, destroyed the cell ultrastructure and decreased transepithelial electrical resistance in HUVECs. In addition, a higher relative migration rate, greater number of migrated cells, stronger tube-forming ability and increased expression of VEGF and VEGFR2 were detected in the Exp_HP group compared with the control group. The properties of HUVECs in Exp_H (co-cultured with H1975 cells) were similar to those in Exp_HP, but significantly weaker. Taken together, the results of the present study suggest that tumor cells interacting with PLTs may play an important role in tumor angiogenesis by affecting or mediating changes in the properties of vascular endothelial cells (VECs).
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- 2021
10. Synthesis of an acrylate constructed by phosphaphenanthrene and triazine-trione and its application in intrinsic flame retardant vinyl ester resin
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Huajun Duan, Xin Wang, Xiaoxiao Tao, Shuang Yang, and Wenjing Dong
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Thermogravimetric analysis ,Acrylate ,Materials science ,Polymers and Plastics ,Vinyl ester ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Mechanics of Materials ,Cone calorimeter ,Materials Chemistry ,Heat of combustion ,Fourier transform infrared spectroscopy ,0210 nano-technology ,Nuclear chemistry ,Fire retardant ,Acrylic acid - Abstract
An intrinsic flame retardant vinyl ester TGIC-AA-DOPO constructed by phosphaphenanthrene and triazine-trione groups was successfully synthesized via the reaction between 1,3,5-triglycidyl isocyanurate (TGIC), acrylic acid and 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO). The structure of TGIC-AA-DOPO was characterized in detail by fourier transform infrared spectroscopy (FTIR), 1 H and 31 P nuclear magnetic resonance (NMR) and liquid chromatography-high resolution mass spectrometry (LC-HRMS). The intrinsic P-N-containing flame retardant vinyl ester resin (IFR-VER) was prepared after diluting TGIC-AA-DOPO with styrene (st) and triallyl isocyanurate (TAIC). The flame retardancy, thermal properties and combustion behavior of IFR-VER were respectively investigated by a series of tests involving limited oxygen index (LOI) measurement, UL-94 vertical burning test, cone calorimeter test, thermogravimetric analysis (TGA) and dynamic mechanical analysis (DMA). The results showed that the as-obtained resin system exhibited a dramatic improvement in flame retardance. In the case of 40 wt% TAIC in resin system, the LOI value achieved 31%, leading to the UL-94 V-0 rating. In addition, compared with the 901-VER, the peak heat release rate (pk-HRR), average of heat release rate (av-HRR), total heat release (THR) and average effective heat of combustion (av-EHC) of the IFR-VER containing 40% TAIC diluent were decreased by 48%, 60%, 41% and 24% respectively. The diphase flame retardant effect of the prepared IFR-VER during combustion was demonstrated by the results of Py-GC/MS, FTIR, laser raman spectroscopy and scanning electron microscope (SEM).
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- 2018
11. Study on the Mechanical Properties of Polyurethane Based on PTMG-TMP System
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Wenjing Dong, Xiaoxiao Tao, Bo Zhang, and Huajun Duan
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Materials science ,Polymers and Plastics ,Toluene diisocyanate ,General Chemical Engineering ,Materials Science (miscellaneous) ,Diol ,Extender ,technology, industry, and agriculture ,Ether ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,law.invention ,chemistry.chemical_compound ,chemistry ,law ,Polymer chemistry ,Materials Chemistry ,heterocyclic compounds ,Trimethylolpropane ,0210 nano-technology ,Polyurethane - Abstract
In this paper, poly (tetrahydrofuranyl ether diol) and trimethylolpropane (TMP) were used respectively as chain extender and cross-linking agent to fabricate polyurethane resin. The morphol...
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- 2018
12. p53-mediated ferroptosis is required for 1-methyl-4-phenylpyridinium-induced senescence of PC12 cells
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Qinglin Li, Yuting Guo, Shanshan Li, Meng Wang, Shasha Tian, Xiaoxiao Tao, Youlin Wang, Yin Cao, and Xuncui Wang
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0301 basic medicine ,Senescence ,1-Methyl-4-phenylpyridinium ,Programmed cell death ,Amino Acid Transport System y+ ,Cell ,Phenylenediamines ,Mitochondrion ,SLC7A11 ,Toxicology ,GPX4 ,PC12 Cells ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,medicine ,Animals ,Ferroptosis ,Cellular Senescence ,Membrane Potential, Mitochondrial ,Cyclohexylamines ,biology ,Chemistry ,General Medicine ,Phospholipid Hydroperoxide Glutathione Peroxidase ,beta-Galactosidase ,Rats ,Cell biology ,Oxidative Stress ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,biology.protein ,Tumor Suppressor Protein p53 ,Signal transduction ,Reactive Oxygen Species - Abstract
Parkinson's disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra and striatum. Aging is the most important risk factor of PD. Ferroptosis is an iron-dependent form of cell death associated with PD. However, it is not clear whether ferroptosis accelerates PD by promoting cellular senescence. This study investigated the mechanism of 1-methyl-4-phenylpyridinium (MPP+) -induced PC12 cells injury. We found that MPP+ induced cell senescence with increased β-galactosidase activity and the expression of p53, p21 and p16 activation in cells. In addition, MPP+ treatment showed smaller mitochondria and increased membrane density, downregulation of ferritin heavy chain 1 expression and upregulation of acyl-CoA synthetase long chain family member 4 expression, and enhanced levels of oxidative stress, which were important characteristics of ferroptosis. Ferrostatin-1 (Fer-1), a ferroptosis inhibitor, was tested to eliminate MPP+-induced cell senescence. Fer-1 downregulated the expression of p53 and upregulated the expression of solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase-4 (GPX4) in MPP+-induced ferroptosis. Inhibition of p53 eliminated cell senescence by upregulation the expression of of SLC7A11 and GPX4. Thus, these results suggest that MPP+ induces senescence in PC12 cells via the p53/ SLC7A11/ GPX4 signaling pathway in the ferroptosis regulation mechanism.
- Published
- 2021
13. Long non-coding RNA MEG3 alters neuronal apoptosis and is associated with poor prognosis in ischemic stroke: based on basic and prospective studies
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Meiping Wang, Wenjuan Chen, Yu Geng, Chenghua Xu, Xiaoxiao Tao, and Yi Zhang
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Background: Ischemic stroke (IS) is a highly heterogeneous disease with variable pathogenesis. Due to the lack of early predictive markers, the mortality rate of IS remains high worldwide. This study focused on the expression pattern of long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) and its prognostic value in IS. Methods: Twenty-four ICR mice (30-35g, 12 males and 12 females) were involved in this study. Middle cerebral artery occlusion (MCAO) was established to simulate an IS environment. Oxygen-glucose deprivation/reoxygenation (OGD/R) in cells were also performed. A total of 215 IS patients and 153 age and gender-matched controls were included. Quantitative Real-time PCR (qRT-PCR) was performed to detect the expression of MEG3 in peripheral blood leukocytes. Results: The MEG3 expression in MCAO group was significantly higher than control group (P=0.004), and the survival time of high MEG3 group was significantly lower than that of the low MEG3 group (P=0.042). OGD/R promotes cell apoptosis, while si-MEG3 inhibits apoptosis. Results of WB showed that, expression level of Bax was down regulated by si-MEG3, while OGD/R increased its expression level. MEG3 was significantly up-regulated in IS patients and associated with the National Institutes of Health Stroke Scale (NIHSS) (r=0.347, P
- Published
- 2019
14. TIGAR suppresses seizures induced by kainic acid through inhibiting oxidative stress and neuronal apoptosis
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Qin Mei, Xiaoxiao Tao, Chunyou Chen, Xuewen Feng, Chenfeng Qiu, Linlin Wang, and Jingang Zhu
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0301 basic medicine ,Male ,Kainic acid ,Biophysics ,Apoptosis ,Oxidative phosphorylation ,Pentose phosphate pathway ,medicine.disease_cause ,Biochemistry ,Hippocampus ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Seizures ,medicine ,Animals ,Glycolysis ,Molecular Biology ,Neurons ,TUNEL assay ,Kainic Acid ,Apoptosis Regulator ,Cell Biology ,Glutathione ,Molecular biology ,Phosphoric Monoester Hydrolases ,Oxidative Stress ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Apoptosis Regulatory Proteins ,Oxidative stress - Abstract
TP53-induced glycolysis and apoptosis regulator (TIGAR) activates the pentose phosphate pathway (PPP), which feeds reduced nicotinamide adenine dinucleotide phosphate (NADPH) to the antioxidant glutathione pathway. Oxidative stress-induced neuronal apoptosis is the pathological basis of several neurological disorders, including epilepsy. To determine the potential anti-epileptic action TIGAR in a rodent kainic acid (KA)-induced seizure model. Seizures were induced by the intra-cerebroventricular injection of KA, followed by injection of empty or TIGAR-expressing lentiviral vectors. Immunofluorescence was used to detect the localization of TIGAR in the cortices and hippocampi, and the expression levels of relevant proteins were determined by Western blotting. Oxidative stress-related markers were detected using commercially available kits. Neuronal apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining. TIGAR were mainly expressed in the neurons and rarely located in the astrocytes, and increased in the cortices and hippocampi of KA-treated rats in a time-dependent manner. Lentivirus-mediated TIGAR overexpression significantly decreased the oxidative stress and neuronal apoptosis induced by KA, resulting in prolonged seizure latency and lower Racine scores. Our findings indicate that TIGAR has anti-epileptic, anti-oxidant and anti-apoptotic effects, and is therefore a promising therapeutictarget for epilepsy.
- Published
- 2019
15. Lower Serum Indirect Bilirubin Levels are Inversely Related to Carotid Intima-Media Thickness Progression
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Xingquan Zhao, Zhimin Wang, Xiaoxiao Tao, Jianwei Wu, Chenghua Xu, and Anxin Wang
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0301 basic medicine ,Adult ,Carotid Artery Diseases ,Male ,medicine.medical_specialty ,030204 cardiovascular system & hematology ,Logistic regression ,Gastroenterology ,Asymptomatic ,Carotid Intima-Media Thickness ,Severity of Illness Index ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,Developmental Neuroscience ,Risk Factors ,Internal medicine ,medicine ,Humans ,Aged ,Ultrasonography ,Univariate analysis ,business.industry ,Confounding ,Bilirubin ,Odds ratio ,Middle Aged ,Atherosclerosis ,Confidence interval ,030104 developmental biology ,Neurology ,Quartile ,Intima-media thickness ,Disease Progression ,Female ,medicine.symptom ,business ,Biomarkers - Abstract
Background:Bilirubin has been recognized as a potential endogenous inhibitor of atherosclerosis, being inversely associated with carotid intima-media thickness (CIMT). However, little information is available concerning the correlation between serum indirect bilirubin (IBIL), especially long-term IBIL level, and early atherosclerosis progression. This study was designed to evaluate the relationship between serum IBIL level and CIMT progression.Methods:A total of 2205 participants were enrolled in this Asymptomatic Polyvascular Abnormalities Community study (APAC study). CIMT was measured at baseline and 2-year follow-up. The participants were divided into four groups based on their serum IBIL levels at baseline. Both baseline and average serum IBIL values during the 2-year follow up were used in the analysis. Multivariable logistic regression and linear regression were used to assess the associations between serum IBIL and CIMT progression.Results:The results showed that 51.93% (1145/2205) of participants were diagnosed with CIMT progression during the 2-year follow-up. Baseline serum IBIL level was significantly associated with the incidence of CIMT progression after adjusting for other potential confounding factors. Compared with the first quartile, adjusted odds ratios (OR) of the second, third, and fourth quartiles of IBIL were 0.70 [95% confidence interval (CI), 0.55-0.90], 0.68 (95% CI, 0.52-0.87), and 0.63 (95% CI, 0.49-0.82) (P = 0.0006), respectively. Serum IBIL level during the follow-up was also associated with CIMT progression in the univariate analysis (P = 0.0022), although no longer significant after adjusting for potential confounders in the multiple linear regression.Conclusion:The study demonstrated the inverse relationship between serum IBIL and CIMT progression. Lower serum IBIL level is an independent predictor of subclinical atherosclerosis.
- Published
- 2019
16. Does resin tapping affect the tree-ring growth and climate sensitivity of the Chinese pine (Pinus tabuliformis) in the Loess Plateau, China?
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Xiaoxiao Tao, Mingyue Hou, Xiaomin Zeng, Lingnan Zhang, Yunxin Chang, Wenchao Wang, Xiaohong Liu, Shiwen Sun, and Youyu Wang
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0106 biological sciences ,Functional ecology ,010504 meteorology & atmospheric sciences ,Ecology ,δ13C ,δ18O ,Growing season ,Plant Science ,01 natural sciences ,Nutrient ,Agronomy ,Palmer drought index ,Dendrochronology ,Tapping ,Environmental science ,010606 plant biology & botany ,0105 earth and related environmental sciences - Abstract
Resin tapping could affect water and nutrient transport processes in Chinese pine trees, rendering them more vulnerable to extreme climatic events, such as drought, and affecting the ecological function of forests in semi-arid regions. This study evaluated how resin tapping affects the tree-ring growth and climate sensitivity of Chinese pine in the Loess Plateau. We compared tree-ring growth patterns between the tapped and untapped faces of tapped trees, and investigated tree-ring growth and its response to climate between tapped and untapped trees in a forest stand during the 1967–2017 period. Tapped trees showed asymmetrical growth patterns after resin tapping, with narrower rings near the tapped face and wider ones near the untapped face. Furthermore, tapped trees had inter-annual variations consistent with those of untapped trees except for the years 2000 and 2001, with significantly lower values following resin tapping, and tree-ring growth then returning to normal. The climate response analysis indicated that the tree-ring growth of both tapped and untapped trees was negatively affected by monthly mean temperatures during the early growing season (May to July) in the post-resin-tapping period. Furthermore, tree-ring growth in tapped trees also revealed significant correlation with water vapour deficit and the Palmer drought index, which indicates that tapped trees are more vulnerable to drought. Further studies based on stable isotopes (i.e. δ13C, δ18O, and δ15N) could improve our understanding of the physiological mechanisms that regulate the effects of resin tapping on tree-ring growth.
- Published
- 2021
17. Calcified decellularized arterial scaffolds impact vascular smooth muscle cell transformation via downregulating α-SMA expression and upregulating OPN expression
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Peng Wang, Xiaoxiao Tao, Huaping Xin, Liling You, Shuwu Wu, Zhimin Wang, and Chenhua Xu
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0301 basic medicine ,Cancer Research ,Vascular smooth muscle ,Decellularization ,biology ,Oncogene ,Chemistry ,Cell ,General Medicine ,Articles ,Molecular medicine ,Cell biology ,Extracellular matrix ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Immunology and Microbiology (miscellaneous) ,030220 oncology & carcinogenesis ,medicine ,biology.protein ,Osteopontin ,Myofibroblast - Abstract
The underlying mechanisms of arterial remodeling (AR) remain unclear. Studies have indicated that decellularized scaffolds stimulate the differentiation of fibroblasts into myofibroblasts and promote the accumulation of the extracellular matrix (ECM). In the present study, the impact of ECM changes following AR on vascular smooth muscle cell (VSMC) phenotypes was investigated. VSMCs were co-cultured with normal or calcified decellularized arterial scaffolds. The expression levels of α-smooth muscle actin (α-SMA) and osteopontin (OPN) were measured at 2, 5, 10, 15 and 21 days following the establishment of the co-culture systems. The expression of α-SMA in the normal co-culture group was significantly increased compared with that in the calcified arterial decellularized scaffold co-culture group (P
- Published
- 2018
18. Long-term hypoxia increases calcium affinity of BK channels in ovine fetal and adult cerebral artery smooth muscle
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Glyne U. Thorington, Sean M. Wilson, David A. Hessinger, Xiaoxiao Tao, Mike T. Lin, and Lawrence D. Longo
- Subjects
medicine.medical_specialty ,BK channel ,Patch-Clamp Techniques ,Large-Conductance Calcium-Activated Potassium Channel beta Subunits ,Physiology ,Acclimatization ,Vascular Biology and Microcirculation ,Myocytes, Smooth Muscle ,Cerebral arteries ,Gestational Age ,Muscle, Smooth, Vascular ,Membrane Potentials ,Pregnancy ,Physiology (medical) ,Internal medicine ,medicine.artery ,medicine ,Basilar artery ,Animals ,Myocyte ,Large-Conductance Calcium-Activated Potassium Channels ,Patch clamp ,Phosphorylation ,Hypoxia ,Large-Conductance Calcium-Activated Potassium Channel alpha Subunits ,Cells, Cultured ,Membrane potential ,Fetus ,Sheep ,biology ,Altitude ,Age Factors ,Anatomy ,Immunohistochemistry ,Disease Models, Animal ,Kinetics ,Electrophysiology ,Endocrinology ,Basilar Artery ,biology.protein ,Calcium ,Female ,Cardiology and Cardiovascular Medicine ,Ion Channel Gating - Abstract
Acclimatization to high-altitude, long-term hypoxia (LTH) reportedly alters cerebral artery contraction-relaxation responses associated with changes in K+ channel activity. We hypothesized that to maintain oxygenation during LTH, basilar arteries (BA) in the ovine adult and near-term fetus would show increased large-conductance Ca2+ activated potassium (BK) channel activity. We measured BK channel activity, expression, and cell surface distribution by use of patch-clamp electrophysiology, flow cytometry, and confocal microscopy, respectively, in myocytes from normoxic control and LTH adult and near-term fetus BA. Electrophysiological data showed that BK channels in LTH myocytes exhibited 1) lowered Ca2+ set points, 2) left-shifted activation voltages, and 3) longer dwell times. BK channels in LTH myocytes also appeared to be more dephosphorylated. These differences collectively make LTH BK channels more sensitive to activation. Studies using flow cytometry showed that the LTH fetus exhibited increased BK β1 subunit surface expression. In addition, in both fetal groups confocal microscopy revealed increased BK channel clustering and colocalization to myocyte lipid rafts. We conclude that increased BK channel activity in LTH BA occurred in association with increased channel affinity for Ca2+ and left-shifted voltage activation. Increased cerebrovascular BK channel activity may be a mechanism by which LTH adult and near-term fetal sheep can acclimatize to long-term high altitude hypoxia. Our findings suggest that increasing BK channel activity in cerebral myocytes may be a therapeutic target to ameliorate the adverse effects of high altitude in adults or of intrauterine hypoxia in the fetus.
- Published
- 2015
19. Solution blowing of activated carbon nanofibers for phenol adsorption
- Author
-
Bowen Cheng, Xiaoxiao Tao, Li Hongjun, Xupin Zhuang, Zhou Guoqing, and Li Xiaojie
- Subjects
Langmuir ,Materials science ,Aqueous solution ,General Chemical Engineering ,Polyacrylonitrile ,General Chemistry ,Microporous material ,chemistry.chemical_compound ,Adsorption ,chemistry ,Chemical engineering ,Polymer chemistry ,medicine ,Freundlich equation ,Texture (crystalline) ,Activated carbon ,medicine.drug - Abstract
An activated carbon nanofiber (ACNF) with high surface area and excellent phenol adsorption capacity has been successfully fabricated by solution blowing of polyacrylonitrile (PAN) into precursor nanofiber which was subsequently activated to ACNF via KOH activation process. The effects of impregnation ratio and activation temperature on the texture properties of ACNF were discussed. The texture properties were characterized by N2 adsorption–desorption isotherm. ACNF showed high value of special surface area and pore volume (2921.263 m2 g−1 and 2.714 cm3 g−1, respectively). The ACNF samples were used for phenol adsorption from aqueous solutions. Adsorption isotherms at 38 °C were fitted with Langmuir and Freundlich models. The relationship between texture properties and phenol adsorption behavior was investigated. As a result, ACNF sample with large surface area, high micropore volume and pore size close and above to 0.43 nm showed the maximum phenol adsorption capacity of 251.6 mg g−1.
- Published
- 2015
20. Fabrication of ZrO2 ceramic fiber mats by solution blowing process
- Author
-
Xiaoxiao Tao, Bowen Cheng, Lei Shi, Guilong Yan, and Xupin Zhuang
- Subjects
Materials science ,Process Chemistry and Technology ,Catalyst support ,Composite number ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,law.invention ,law ,visual_art ,Materials Chemistry ,Ceramics and Composites ,visual_art.visual_art_medium ,Calcination ,Cubic zirconia ,Fiber ,Ceramic ,Composite material ,Filtration ,Sol-gel - Abstract
ZrO 2 is an important ceramic material, which could be applied into many fields including structural materials, filtration, catalyst support, solar cells and batteries. This paper reported that ZrO 2 fiber mats can be fabricated by solution blowing process and a subsequent calcination process. During the solution blowing process, as-spun PVP/ZrOCl 3 composite fibers were formed after solvent evaporation and stretching of gas flow. These fibers could be subsequently converted into ZrO 2 fibers via calcination process in air at 800 °C. Diameter of these ZrO 2 ceramic fibers could be controlled in the range from 8 to 20 μm by changing parameters, such as the PVP concentration, ZrOCl 3 concentration, gas pressure, the needle size, and the injection rate of the precursor solution.
- Published
- 2014
21. A Retrospective Study of Clinical Outcomes After Endovascular Treatment in Acute Ischemic Stroke Patients with Complete Anterior Circulation Infarction in the Absence of Multimodal Computed Tomography
- Author
-
Ying Jiangxian, Yi Zhang, Huaping Xin, Zhimin Wang, Anyang Tao, Chenghua Xu, Peng Wang, Xian Zheng, and Xiaoxiao Tao
- Subjects
Brain Infarction ,Male ,medicine.medical_specialty ,Time Factors ,medicine.medical_treatment ,Perfusion scanning ,Severity of Illness Index ,03 medical and health sciences ,0302 clinical medicine ,Modified Rankin Scale ,medicine ,Humans ,cardiovascular diseases ,030212 general & internal medicine ,Stroke ,Aged ,Retrospective Studies ,Aged, 80 and over ,medicine.diagnostic_test ,business.industry ,Total anterior circulation infarct ,Endovascular Procedures ,Atrial fibrillation ,Digital subtraction angiography ,Thrombolysis ,Middle Aged ,medicine.disease ,Prognosis ,Surgery ,Treatment Outcome ,Angiography ,Female ,Neurology (clinical) ,business ,030217 neurology & neurosurgery - Abstract
Objective To analyze the positive predictive value of large artery occlusion and clinical prognosis in acute ischemic stroke patients with total anterior circulation infarct (TACI) who underwent endovascular treatment in the absence of multimodal CT angiography or CT perfusion. Methods The inclusion criteria for the acute ischemic stroke patients to receive endovascular treatment were as the follows: the Oxfordshire Community Stroke Project classification was TACI, Alberta Stroke Program Early Computed Tomography Score (ASPECTS) ≥ 6, National Institutes of Health stroke scale (NIHSS) score ≥8, and less than 4.5 hours since stroke onset. The endovascular treatment was performed on patients who met the inclusion criteria. The endovascular treatment included intra-arterial thrombolysis, mechanical treatments, or both. A retrospective analysis was performed on all eligible acute ischemic stroke patients who underwent endovascular treatment from January 1, 2015 to December 31, 2015. Results A total of 17 patients met the inclusion criteria and underwent endovascular treatment. The median age was 76 years (range, 59–88 years). 12 patients (70.6%) were diagnosed with atrial fibrillation. 16 patients were diagnosed with large artery occlusion by digital subtraction angiography, and the positive predictive value was 94.1%. 16 patients (94.1%) had recanalization (TICI Grade 3); 12 patients (70.6%) had a modified Rankin Scale score of 0–2, and 1 patient (5.9%) died 90 days after treatment. Conclusions In the absence of multimodal CT, endovascular treatment might be beneficial to patients with TACI acute ischemic stroke within 4.5 hours of stroke onset, who had NIHSS score of 8 or greater and ASPECTS of 6 or greater. These inclusion criteria have a high positive predictive value for anterior circulation large artery occlusion.
- Published
- 2016
22. UV‐curable polyurethane acrylate resin containing multiple active terminal groups for enhanced mechanical properties
- Author
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Wenjing Dong, Xin Wang, Xiaoxiao Tao, Huajun Duan, and Huiru Ma
- Subjects
chemistry.chemical_compound ,Acrylate ,Materials science ,Polymers and Plastics ,chemistry ,Terminal (electronics) ,Polymer chemistry ,Materials Chemistry ,General Chemistry ,Surfaces, Coatings and Films ,Polyurethane - Published
- 2019
23. Phosphorus–nitrogen‐type fire‐retardant vinyl ester resin with good comprehensive properties
- Author
-
Teng Yin, Huajun Duan, Yongsheng Chen, Sa Ji, Huiru Ma, and Xiaoxiao Tao
- Subjects
Materials science ,Polymers and Plastics ,Phosphorus ,Radical polymerization ,Vinyl ester ,chemistry.chemical_element ,Thermosetting polymer ,General Chemistry ,Nitrogen ,Surfaces, Coatings and Films ,chemistry ,Materials Chemistry ,Copolymer ,Organic chemistry ,Fire retardant - Published
- 2019
24. Solution blowing nylon 6 nanofiber mats for air filtration
- Author
-
Xiaoxiao Tao, Lei Shi, Bowen Cheng, Xupin Zhuang, and Weimin Kang
- Subjects
Pressure drop ,Air filtration ,Materials science ,Polymers and Plastics ,General Chemical Engineering ,General Chemistry ,law.invention ,Filter (aquarium) ,chemistry.chemical_compound ,Nylon 6 ,chemistry ,law ,High productivity ,Nanofiber ,Composite material ,Spinning ,Filtration - Abstract
Solution blowing process is a new nanofiber fabricating method with high productivity. In the present study, nylon 6 nanofiber mats were solution blown and the effects of spinning conditions on nanofibers morphology were investigated. The fiber diameter ranged from 150 to 750 nm which was affected by solution concentration, gas pressure and solution feeding rate. The solution blown fibers were three-dimensional curly which made loose construction in bulk. The filtration performance of solution blown mats was evaluated. The tested solution blown nanofiber mats showed high filtration efficiency of 83.10 % to 93.45 % for 0.3 µm particles filtration and extremely low pressure drop of 15.37 to 30.35 Pa. The results indicate the solution blown nanofiber mats will find potential application of high efficiency and low resistance filter.
- Published
- 2013
25. Solution Blowing of Silicon Carbide nanofiber and its thermal stability
- Author
-
Bowen Cheng, Guilong Yan, Xiaoxiao Tao, and Xupin Zhuang
- Subjects
chemistry.chemical_compound ,Materials science ,chemistry ,Nanofiber ,Silicon carbide ,General Materials Science ,Thermal stability ,Composite material - Published
- 2013
26. Ontogeny, ryanodine receptor‐mediated calcium sparks, and BK channel clustering in basilar arterial myocytes from long‐term hypoxic sheep (853.9)
- Author
-
Lawrence D. Longo, Shane Glasgow, Sean M. Wilson, Donald M. Bers, Noah Osman, Leighton T. Izu, Xiaoxiao Tao, Jose L. Puglisi, Monica Romero, and David A. Hessinger
- Subjects
medicine.medical_specialty ,BK channel ,Fetus ,biology ,Ryanodine receptor ,Chemistry ,Ontogeny ,Hypoxia (medical) ,equipment and supplies ,musculoskeletal system ,Biochemistry ,BK channel activity ,Calcium sparks ,fluids and secretions ,Endocrinology ,Internal medicine ,cardiovascular system ,Genetics ,medicine ,biology.protein ,Myocyte ,medicine.symptom ,tissues ,Molecular Biology ,Biotechnology - Abstract
Ryanodine receptor (RyR) activity causes Ca2+ sparks in cerebral arterial myocytes by activating adjacent Ca2+-activated K+ (BK) channels, which can dilate arteries. Previous studies show ontogeny increases Ca2+ sparks and long-term hypoxia (LTH) increases BK channel activity. We hypothesized that BK channel activity is greater in arterial myocytes of the LTH fetus than adult due to greater spark activity, and because BK channel clustering is greater in regions where sparks occur. To address this hypothesis we examined spark activity and BK channel clustering to sites of potential spark activity. This was accomplished using line-scan and immunofluorescence techniques, respectively, in basilar arteries from LTH fetal (FH) and adult sheep (AH) kept at 3,801 m for > 100 days. The percentage of myocytes with sparks was ~ 2-fold greater in FH versus AH and unaffected by 30 mM K (30K). Spark amplitude was 7% greater in FH 30K versus AH 30K, while no spatial or temporal changes to sparks were observed. BK channe...
- Published
- 2014
27. Long-term hypoxia increases calcium affinity of BK channels in ovine fetal and adult cerebral artery smooth muscle.
- Author
-
Xiaoxiao Tao, Lin, Mike T., Thorington, Glyne U., Wilson, Sean M., Longo, Lawrence D., and Hessinger, David A.
- Subjects
- *
HYPOXEMIA , *POTASSIUM channels , *ANTERIOR cerebral artery , *BASILAR artery , *CELL membranes , *CONFOCAL microscopy - Abstract
Acclimatization to high-altitude, long-term hypoxia (LTH) reportedly alters cerebral artery contraction-relaxation responses associated with changes in K channel activity. We hypothesized that to maintain oxygenation during LTH, basilar arteries (BA) in the ovine adult and near-term fetus would show increased large-conductance Ca2+ activated potassium (BK) channel activity. We measured BK channel activity, expression, and cell surface distribution by use of patch-clamp electrophysiology, flow cytometry, and confocal microscopy, respectively, in myocytes from normoxic control and LTH adult and nearterm fetus BA. Electrophysiological data showed that BK channels in LTH myocytes exhibited 1) lowered Ca2+ set points, 2) left-shifted activation voltages, and 3) longer dwell times. BK channels in LTH myocytes also appeared to be more dephosphorylated. These differences collectively make LTH BK channels more sensitive to activation. Studies using flow cytometry showed that the LTH fetus exhibited increased β1 subunit surface expression. In addition, in both fetal groups confocal microscopy revealed increased BK channel clustering and colocalization to myocyte lipid rafts. We conclude that increased BK channel activity in LTH BA occurred in association with increased channel affinity for Ca2+ and left-shifted voltage activation. Increased cerebrovascular BK channel activity may be a mechanism by which LTH adult and near-term fetal sheep can acclimatize to long-term high altitude hypoxia. Our findings suggest that increasing BK channel activity in cerebral myocytes may be a therapeutic target to ameliorate the adverse effects of high altitude in adults or of intrauterine hypoxia in the fetus. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
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