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23 results on '"Xie, Jiuxiang"'

13. Integrated transcriptomic and metabolomic analyses of Caucasian clover (Trifolium ambiguumBieb.) in response to freezing stress

Author

Mou, Dan, Li, Zongren, Liu, Wenhui, Liang, Guoling, Jia, Zhifeng, Yu, Hongyan, Wang, Jiuluan, Ou, Weiyou, Liu, Kai, Yao, Xixi, Zhan, Yu, Ma, Geliang, Kazhuo, Cairang, Li, Xilai, and Xie, Jiuxiang

Abstract

Caucasian clover (Trifolium ambiguumBieb.) is a perennial leguminous herbage with unique properties, such as resistance to cold, drought, and grazing. However, the response of Caucasian clover to freezing stress based on gene expression level and metabolite accumulation is unknown. This study performed transcriptomic and metabolomic analyses to investigate the response mechanism of Caucasian clover under freezing stress. Transcriptome analysis detected 6792 differentially expressed genes (DEGs) in Caucasian clover under freezing stress. These DEGs were significantly enriched in carbohydrate and energy metabolism, lipid metabolism, and biosynthesis of secondary metabolites. Meanwhile, 139 differentially accumulated metabolites (DAMs) were identified in Caucasian clover under freezing stress. These DAMs were mainly enriched in amino acid metabolism and biosynthesis of secondary metabolites. Furthermore, the gene-to-metabolite network was used to determine the genes and metabolites involved in polyphenol biosynthesis and osmoprotectants metabolism. Therefore, this study provides insights into the complex regulatory strategy of Caucasian clover response to freezing stress.

Published
2022
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14. Quantitative Analyses of Nine Phenolic Compounds and Their Antioxidant Activities from Thirty-Seven Varieties of Raspberry Grown in the Qinghai-Tibetan Plateau Region

Author

Guangxiang Luan, Xie Jiuxiang, Yixi Zhuoma, Liang Jian, Wu Zhou, Wang Yuwei, and Shoude Zhang

Subjects
rsm, Pharmaceutical Science, antioxidant activity, phenols, Tibet, 01 natural sciences, Article, Antioxidants, Analytical Chemistry, Ferulic acid, rp-hplc/uv, lcsh:QD241-441, chemistry.chemical_compound, 0404 agricultural biotechnology, Picrates, Chlorogenic acid, lcsh:Organic chemistry, Limit of Detection, Drug Discovery, Vanillic acid, Humans, Benzothiazoles, Food science, Gallic acid, Phenols, Physical and Theoretical Chemistry, Chromatography, High Pressure Liquid, Chemistry, Biphenyl Compounds, 010401 analytical chemistry, Organic Chemistry, Extraction (chemistry), Reproducibility of Results, Free Radical Scavengers, Hep G2 Cells, 04 agricultural and veterinary sciences, Reference Standards, Syringic acid, 040401 food science, 0104 chemical sciences, Blowing a raspberry, Chemistry (miscellaneous), Linear Models, Molecular Medicine, Sulfonic Acids, Rubus, raspberry
Abstract

In this work, an efficient method for the rapid extraction and separation of antioxidant phenols was developed and optimized. The method was then applied to extract and separate nine phenols from 37 varieties of raspberry, in which their antioxidant activities were further investigated. First, the extraction was conducted using ultra-sonication, which was then further separated using reversed-phase high-performance liquid chromatography/ultraviolet (RP-HPLC/UV) analysis. In this step, several key parameters (volume of the extraction reagent, time of extraction, and the temperature of extraction) affecting its efficiency were investigated and optimized using the response surface methodology (RSM) combined with the Box&ndash, Behnken design (BBD) so that the optimal conditions were obtained. According to the overall results of the optimization study, the optimal conditions were chosen as follows: volume of extraction reagent = 2.0 mL, time of extraction = 50.0 min, and temperature of extraction = 50 &deg, C. The optimal conditions were then applied to extract nine phenols, including gallic acid, catechin, chlorogenic acid, vanillic acid, syringic acid, cumaric acid, ferulic acid, rosemary acid, and quercetin from 37 raspberry varieties. The extracted phenols were characterized and their antioxidant activities, including DPPH&minus, and ABTS&minus, free radical scavenging and intracellular reactive oxygen species (ROS) activity, using HepG2 cells as the model, were subsequently studied. The findings suggested that although their contents varied among most raspberry varieties, these phenols significantly contributed toward their antioxidant capacity and scavenging intracellular ROS activities. This study provides a scientific and theoretical basis for the selection of raspberry varieties and product development in Qinghai province.

Published
2019

17. Characteristics of complete chloroplast genome of a high-quality forage on Qinghai-Tibet Plateau, Medicago archiducis-nicolai Sirj. (Fabaceae：Trifolieae).

Author

Xie, Jiuxiang, Liang, Chengbo, Li, Zongren, Ji, Zhongke, Ou, Weiyou, Liu, Hua, Wang, Haichun, Kazhuocairang, and Hou, Zhiqiang

Subjects
CHLOROPLAST DNA, MEDICAGO, DROUGHT tolerance, LEGUMES
Abstract

Medicago archiducis-nicolai Sirj. is a well-known high-quality forage as its good palatability and strong tolerance to drought, cold and saline-alkali stress. Here, the complete chloroplast genome sequence of M. archiducis-nicolai was reported. The size of the complete chloroplast genome is 127,072 bp in length. The chloroplast genome has no inverted repeat (IR) regions, which is very common in the family Fabaceae. The M. archiducis-nicolai chloroplast genome encodes 106 genes: 72 protein-coding genes, 30 tRNAs, and 4 rRNAs. The phylogenetic analysis result strongly suggested that M. archiducis-nicolai is a distinct lineage in Medicago, being sister to highly supported clade composed of three species (M. hybrida, M. papillosa and M. sativa). [ABSTRACT FROM AUTHOR]

Published
2021
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20. Complete chloroplast genome of a high-quality forage in north China, Medicago ruthenica (Fabaceae：Trifolieae).

Author

Xie, Jiuxiang, Mao, Junping, Li, Zongren, Liang, Chengbo, Ou, Weiyou, Tang, Junwei, Liu, Hua, Wang, Haichun, Ji, Zhongke, Kazhuocairang, and Shen, Yingfang

Subjects
CHLOROPLAST DNA, MEDICAGO, DROUGHT tolerance, TRANSFER RNA, LEGUMES
Abstract

Medicago ruthenica is a well-known high-quality forage due to its good palatability and strong tolerance to drought, cold and saline-alkali stress. Here, the complete chloroplast genome sequence of M. ruthenica was reported. The chloroplast genome is 126,939 bp in length. This chloroplast genome has no inverted repeat (IR) regions, which is very common in the family Fabaceae. The M. ruthenica chloroplast genome encodes 107 genes, including 73 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Phylogenetic analysis result strongly suggested that M. ruthenica is a distinct lineage in Medicago, being sister to highly supported clade composed of three species (M. hybrida, M. papillosa and M. sativa). [ABSTRACT FROM AUTHOR]

Published
2021
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21. A new species of Australobius(Lithobiomorpha: Lithobiidae) from the Qinghai-Tibet Plateau, China

Author

Qin, Wen, Lin, Gonghua, Zhao, Xianxian, Li, Bang, Xie, Jiuxiang, Ma, Huiqin, Su, Jianping, and Zhang, Tongzuo

Abstract

A new species of AustralobiusChamberlin, 1920 (Lithobiidae: Lithobiinae), from Jiuzhaigou of the Qinghai-Tibet Plateau is described as Australobius apicicornissp. n. The new species is distinguished from two recorded congeneric species from the Qinghai-Tibet Plateau by having 24 antennal segments, eight ocelli, lacking porodonts, and with accessory claws in 14 legs. A key to the Chinese species of Australobiusis presented.

Published
2014
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22. Characteristics of complete chloroplast genome of a high-quality forage on Qinghai-Tibet Plateau, Medicago archiducis-nicolai Sirj. (Fabaceae：Trifolieae).

Author

Xie J, Liang C, Li Z, Ji Z, Ou W, Liu H, Wang H, Kazhuocairang, and Hou Z

Abstract

Medicago archiducis-nicolai Sirj. is a well-known high-quality forage as its good palatability and strong tolerance to drought, cold and saline-alkali stress. Here, the complete chloroplast genome sequence of M. archiducis-nicolai was reported. The size of the complete chloroplast genome is 127,072 bp in length. The chloroplast genome has no inverted repeat (IR) regions, which is very common in the family Fabaceae. The M. archiducis-nicolai chloroplast genome encodes 106 genes: 72 protein-coding genes, 30 tRNAs, and 4 rRNAs. The phylogenetic analysis result strongly suggested that M. archiducis-nicolai is a distinct lineage in Medicago , being sister to highly supported clade composed of three species ( M. hybrida, M. papillosa and M. sativa )., Competing Interests: No potential conflict of interest was reported by the authors., (© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)

Published
2021
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23. Complete chloroplast genome of a high-quality forage in north China, Medicago ruthenica (Fabaceae：Trifolieae).

Author

Xie J, Mao J, Li Z, Liang C, Ou W, Tang J, Liu H, Wang H, Ji Z, Kazhuocairang, and Shen Y

Abstract

Medicago ruthenica is a well-known high-quality forage due to its good palatability and strong tolerance to drought, cold and saline-alkali stress. Here, the complete chloroplast genome sequence of M. ruthenica was reported. The chloroplast genome is 126,939 bp in length. This chloroplast genome has no inverted repeat (IR) regions, which is very common in the family Fabaceae. The M. ruthenica chloroplast genome encodes 107 genes, including 73 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Phylogenetic analysis result strongly suggested that M. ruthenica is a distinct lineage in Medicago , being sister to highly supported clade composed of three species ( M. hybrida, M. papillosa and M. sativa )., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)

Published
2021
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