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Purpose: The purpose of this study was to evaluate the outcomes of displaced, multi-fragmentary patella fractures in elderly patients treated with anterior plating and cerclage wires. Patients and methods: Between 2018 and 2020, patients aged at least 60 years old undergoing anterior plating and circumferential wiring for displaced, multi-fragmentary patella fractures with a minimum of 12 months follow-up were included. Fragments were reduced and circumferentially stabilized by loop wires, followed by multi-planar fixation with an anterior locking plate and multi-directional screws. Postoperative outcomes were evaluated with the Böstman scores and range of motion. Patients were also asked about symptomatic hardware as well as satisfaction with postoperative outcomes. Results: Sixteen patients with an average age of 71.8 ± 9.3 years old were followed up for an average follow-up was 25.1 ± 8.2 months. At the latest follow-up, the average Böstman scores were 27.2 ± 3.4, and active knee flexion was 123 ± 14°. Two patients complained pinpoint implant irritation and underwent implant removal. Another underwent implant removal due to cultural reasons. Fifteen patients were satisfied with the operative outcomes, and one was unsatisfied due to intermittent patellofemoral pain. Cerclage wire breakage was noted in nine patients on postoperative radiographs, but none elicited pinpoint pain. No wound complications, infections, nonunion or loss of reduction were observed. Conclusions: Anterior locking plates and cerclage wires in tandem provide reliable multi-planar fixation for displaced, multi-fragmentary patellar fractures in elderly patients and resulted in favorable clinical outcomes. [ABSTRACT FROM AUTHOR]

For multilayer perceptron (MLP), the initial weights will significantly influence its performance. Based on the enhanced fractional derivative extend from convex optimization, this paper proposes a fractional gradient descent (RFGD) algorithm robust to the initial weights of MLP. We analyze the effectiveness of the RFGD algorithm. The convergence of the RFGD algorithm is also analyzed. The computational complexity of the RFGD algorithm is generally larger than that of the gradient descent (GD) algorithm but smaller than that of the Adam, Padam, AdaBelief, and AdaDiff algorithms. Numerical experiments show that the RFGD algorithm has strong robustness to the order of fractional calculus which is the only added parameter compared to the GD algorithm. More importantly, compared to the GD, Adam, Padam, AdaBelief, and AdaDiff algorithms, the experimental results show that the RFGD algorithm has the best robust performance for the initial weights of MLP. Meanwhile, the correctness of the theoretical analysis is verified. [ABSTRACT FROM AUTHOR]

It is valuable and meaningful to suppress impulsive noise in system identification. Existing algorithms usually only consider impulsive noise with small frequency and amplitude. Furthermore, few researchers pay attention to the tracking performance of these algorithms. This paper builds a framework based on the deep deterministic policy gradient (DDPG) algorithm with the ability to explore and correct. The enhanced fractional derivative is introduced to further improve the performance of this reinforcement learning-based framework. Thus a fractional least mean square filter algorithm based on reinforcement learning (FrlMS) is proposed. The stability of the FrlMS algorithm is analyzed. Compared with the competing algorithms, the simulation experiments in system identification show that the FrlMS algorithm has satisfactory tracking performance in the face of impulsive noise, especially when the frequency and (or) amplitude are (is) large. [ABSTRACT FROM AUTHOR]

Complex-valued neural network is a kind of learning model which can deal with problems in complex domain. Fully complex extreme learning machine (CELM) is a much faster training algorithm than the complex backpropagation (CBP) scheme. However, it is at the cost of using more hidden nodes to obtain the comparable performance. An upper-layer-solution-aware algorithm has been proposed for training single-hidden layer feedforward neural networks, which performs much better than its counterparts, pseudo-inverse learning (PIL)/extreme learning machine and gradient decent-based backpropagation neural networks. Consequently, there exist two challenges that need to be dealt with: 1) How to combine the advantages of CBP and CELM to develop a novel complex learning algorithm? and 2) What is the convergent behavior of the presented algorithm? In this article, an input weights dependent complex-valued (IWDCV) learning algorithm based on Wirtinger calculus has been proposed, which effectively solves the nonanalytic problem of the common activation functions during training neural networks. In addition, the monotonicity of the error function and the deterministic convergence of the proposed model have been strictly proved, which theoretically guarantee the efficiency and effectiveness of the given model, IWDCV. Finally, for real and complex-valued problems, a variety of simulations have been done to demonstrate the comparable performance of the proposed algorithm which support the theoretical observations as well. [ABSTRACT FROM AUTHOR]

Mesenchymal stem cell‐derived small extracellular vesicles (MSC‐sEVs) possess a great therapeutical potential for osteoarthritis (OA) treatment. However, the steric and electrostatic hindrance of cartilage matrix leads to very limited distribution of MSC‐sEVs in cartilage and low bioavailability of MSC‐sEVs after intra‐articular injection. To overcome this, a strategy to reverse the surface charge of MSC‐sEVs by modifying the MSC‐sEVs with a novel cationic amphiphilic macromolecule namely ε‐polylysine‐polyethylene‐distearyl phosphatidylethanolamine (PPD) was developed in this study. Through incubation with 100 μg/ml PPD, positively charged MSC‐sEVs (PPD‐sEVs) were obtained, and the modification process showed nearly no disturbance to the integrity and contents of sEVs and exhibited good stability under the interference of anionic macromolecules. A more effective cellular uptake and homeostasis modulation ability of PPD‐sEVs than unmodified MSC‐sEVs to chondrocytes was demonstrated. More importantly, PPD‐sEVs demonstrated significantly enhanced cartilage uptake, cartilage penetration, and joint retention capacity as compared to MSC‐sEVs. Intra‐articular injection of PPD‐sEVs into a mouse OA model showed significantly improved bioavailability than MSC‐sEVs, which resulted in enhanced therapeutic efficacy with reduced injection frequency. In general, this study provides a facile and effective strategy to improve the intra‐articular bioavailability of MSC‐sEVs and has a great potential to accelerate the clinical practice of MSC‐sEVs based OA therapy. [ABSTRACT FROM AUTHOR]

Elman recurrent network is a representative model with feedback mechanism. Although gradient descent method has been widely used to train Elman network, it frequently leads to slow convergence. According to optimization theory, conjugate gradient method is an alternative strategy in searching the descent direction during training. In this paper, an efficient conjugate gradient method has been presented to reach the optimal solution in two ways: (1) constructing a more effective conjugate coefficient, (2) determining adaptive learning rates in terms of the generalized Armijo search method. Experiments show that the performance of the new algorithm is superior to traditional algorithms, such as gradient descent method and conjugate gradient method. In particular, the new algorithm has better performance than the evolutionary algorithm. Finally, we prove the weak and strong convergence of the presented algorithm, i.e., the gradient norm of the error function with respect to the weight vectors converges to zero and the weight sequence approaches a fixed optimal point. [ABSTRACT FROM AUTHOR]

Background and purpose There is no consensus on the difference in effects of internal fixation (IF) and external fixation (EF) on outcomes for the treatment of distal radius fractures. We performed a meta-analysis of randomized clinical studies. Methods We searched the literature and included studies that compared the effects of IF and EF on the treatment of distal radius fractures. Statistically, we pooled patient data using standard meta-analytic methods. For the continuous variables, the weighted mean difference (WMD) was used. For dichotomous data, the relative risk (RR) was calculated. Results 10 studies were eligible for data extraction. The pooled data showed that compared with EF, IF led to statistically significantly better Disabilities of the Arm, Shoulder, and Hand (DASH) scores at 12 months postoperatively, recovery of forearm supination at 3 months, and restoration of volar tilt and radial inclination. IF using volar locking plates resulted in better DASH scores than EF at 3 and 6 months, but the trend diminished over time; at 12 months postoperatively, the scores were not statistically significant. Compared with EF, IF led to fewer minor surgical complications. Interpretation For surgical treatment of distal radius fractures, IF yields better functional outcomes, forearm supination, restoration of anatomic volar tilt and radial inclination, and fewer minor complications. The patients who received IF using volar locking plates for the treatment of distal radius recovered more quickly than did patients who received EF. [ABSTRACT FROM AUTHOR]

Abstract: The aims of this study were to (1) determine whether platelet-rich plasma (PRP) could be prepared as a bioactive scaffold capable of endogenous growth factor release for cartilage repair; (2) compare the chondrogenic differentiation ability of mesenchymal stem cells (MSCs) from bone marrow (BMSC) and from adipose (ADSC) seeded within the PRP scaffold; and (3) test the efficacy of ADSC-PRP construct in cartilage regeneration in vivo. In vitro evaluation showed that a 3-dimensional scaffold with a mesh-like microstructure was formed from PRP, with the capability of endogenous growth factor release and ready cell incorporation. Upon seeding in the PRP scaffold, BMSC showed higher proliferation rate, and higher expression of cartilage-specific genes and proteins than ADSC. In an osteochondral defect model in rabbits, implanted BMSC seeded within PRP scaffold also exhibited better gross appearance and histological and immunohistochemical characteristics, higher cartilage-specific gene and protein expression as well as subchondral bone regeneration. ADSC seeded constructs developed into functional chondrocytes secreting cartilaginous matrix in rabbits at 9 weeks post-implantation. Our findings suggest that PRP is a candidate bioactive scaffold capable of releasing endogenous growth factors and that BMSC and ADSC seeded within the PRP scaffold differentiate into chondrocytes and may be suitable for cell-based cartilage repair. [Copyright &y& Elsevier]

Neglected femoral neck fractures in young adults pose a great challenge to orthopedic surgeons because of the high risks of nonunion and osteonecrosis. We attempted to determine whether free vascularised fibular grafting through an anterior approach could enhance bone union, improve hip function and reduce complications in the treatment of such fractures. Thirteen patients with neglected femoral neck fractures were treated with a free vascularised fibular graft through an anterior approach between 2004 and 2008. The mean age was 30.9 years (range, 17-47 years). The average delay between injury and operation was 6.7 months (range, 1.5-22 months). All 13 cases had ununited fractures but without osteonecrosis of the femoral head on plain radiographs before coming under our care. The average follow-up was 51.2 months (range, 36-75 months). All patients had fracture union within an average of 4.8 months (range, 3-9 months). Postoperatively, 9 patients had coxa vara of 10 to 20 degrees and leg discrepancy between 0.5 to 1.5 cm. One patient had coxa vara deformity of 25 degrees and 2-cm leg discrepancy. No patients developed osteonecrosis of the femoral head or donor- site morbidity. The mean Harris hip score improved from 55.5 points preoperatively to 84.8 points postoperatively (p<0.01). This procedure may be useful and safe in the treatment of neglected femoral neck fractures in young adults, but further studies with a large number of patients are needed. [ABSTRACT FROM AUTHOR]

Osteoarthritis (OA) is a common degenerative joint disease which currently lacks of effective agents. It is therefore urgent and necessary to seek an effective approach that can inhibit inflammation and promote cartilage matrix homeostasis. Cartilage progenitor cells (CPCs) are identified as a cell population of superficial zone in articular cartilage which possess strong migration ability, proliferative capacity, and chondrogenic potential. Recently, the application of CPCs may represent a novel cell therapy strategy for OA treatment. There is growing evidence that extracellular vesicles (EVs) are primary mediators of the benefits of stem cell-based therapy. In this study, we explored the protective effects of CPCs-derived EVs (CPCs-EVs) on IL-1β-induced chondrocytes. We found CPCs-EVs exhibited chondro-protective effects in vitro. Furthermore, our study demonstrated that CPCs-EVs promoted matrix anabolism and inhibited inflammatory response at least partially via blocking STAT3 activation. In addition, liquid chromatography-tandem mass spectrometry analysis identified 991 proteins encapsulated in CPCs-EVs. By bioinformatics analysis, we showed that STAT3 regulatory proteins were enriched in CPCs-EVs and could be transported to chondrocytes. To promoting the protective function of CPCs-EVs in vivo, CPCs-EVs were modified with cationic peptide ε-polylysine-polyethylene-distearyl phosphatidylethanolamine (PPD) for surface charge reverse. In posttraumatic OA mice, our results showed PPD modified CPCs-EVs (PPD-EVs) effectively inhibited extracellular matrix catabolism and attenuated cartilage degeneration. Moreover, PPD-EVs down-regulated inflammatory factors expressions and reduced OA-related pain in OA mice. In ex-vivo cultured OA cartilage explants, PPD-EVs successfully promoted matrix anabolism and inhibited inflammation. Collectively, CPCs-EVs-based cell-free therapy is a promising strategy for OA treatment. [ABSTRACT FROM AUTHOR]

Find the global optimal solution of the model is one promising research topic in computational intelligent community. Dependent on analogies to natural processes, the evolutionary swarm intelligent algorithms are widely used for solving global optimization problems which directed by the fitness values. In this paper, we propose one efficient fractional global learning machine (Fragmachine) which includes two stages (descending and ascending) to determine the optimal search path. The neural network model is used to approach the given fitness value. Specifically, for the descending stage, the integer gradient of the network output with respect the current location is employed to find the next descending point, while for the ascending stage, the fractional gradient is implemented to climb and escape from the local optimal point. We further propose one adaptive learning rate during training which relies on both the current gradient (integer or fractional) information and the fitness value. Finally, a series of numerical experiments verify the effectiveness of the proposed algorithm, Fragmachine. [ABSTRACT FROM AUTHOR]

Background: Kashin–Beck disease (KBD) is an endemic, chronic osteoarthropathy that seriously affects joint function and can lead to severe knee deformity. Osteotomy is considered to be one of the effective methods for the treatment of this disease. Therefore, we designed a novel type of osteotomy named combined proximal tibial osteotomy (CPTO), which combines the characteristics of opening‐wedge high tibial osteotomy and tibial condylar valgus osteotomy. Case presentation: We report the case of a 48‐year‐old male with knee pain and varus deformity who was diagnosed with KBD and varus knee osteoarthritis (Kellgren–Lawrence stage IV). Considering the patient's relatively young age, a varus deformity of the right knee of 16.79°, and an intra‐articular instability, we performed a CPTO treatment. In this procedure, we performed an L‐shaped osteotomy from the medial edge of the proximal tibia to the intercondylar eminence and an osteotomy from the medial side of the proximal tibia to the lateral side through the same incision, to adjust the leg alignment and the congruity of the joint by valgus correction. At 29 months follow‐up, this patient achieved satisfactory results, with a varus right knee of 2.87°. There was significant improvement in his right knee function, pain, and joint stability. Conclusions: CPTO may be an acceptable treatment for KBD patients with severe knee varus deformity and intra‐articular instability. It can be considered as an alternative treatment, especially for patients with advanced osteoarthritis needing knee preservation. [ABSTRACT FROM AUTHOR]

Background: Knee osteoarthritis (OA) is a leading cause of global disability. According to current guidelines, exercise is the most recommended and important non-surgical treatment for knee OA. However, the best type of exercise for this condition remains unclear. Evidence has shown that traditional Chinese exercises may be more effective. Therefore, the current prospective, two-armed, single-center randomized controlled trial (RCT) aimed to identify an effective physiotherapy for knee OA.Methods/design: In total, 114 patients with painful knee OA will be recruited from the orthopedic outpatient department of Shanghai Jiao Tong University Affiliated Sixth People's Hospital. To compare the therapeutic effect of two different home-based exercise programs, the participants will be randomly assigned into the experimental group (leg swing exercise) or the control group (quadriceps strengthening exercise). Each participant in both groups will be required to attend five individual sessions with a physiotherapist who will teach the exercise program and monitor progress. Participants will be instructed to perform the exercises at home every day for 12 weeks. Clinical outcomes will be assessed at baseline and 12 and 24 weeks after starting the intervention. The primary outcomes are average overall knee pain and physical function in daily life. The secondary outcomes include other measures of knee pain, physical function, patient-perceived satisfactory improvement, health-related quality of life, physical activity and performance, muscle strength of the lower limb, and adherence.Discussion: This study will provide more evidence on the effects of traditional Chinese exercise on improving physical function and relieving joint pain among patients with knee OA. If proven effective, leg swing exercise can be used as a non-surgical treatment for knee OA in the future.Trial Registration: Chinese Clinical Trial Registry ChiCTR2000039005 . Registered on 13 October 2020. [ABSTRACT FROM AUTHOR]

Platelet aggregates, such as PRP, PRF, and CGF, have been used alone or in combination with other grafting materials to enhance restoration outcomes. The process for preparing these autografting materials requires two-step centrifugation or specific centrifuges. In this study, we obtained an injectable fibrin scaffold (IFS) rich in growth factors by one-step centrifugation of whole blood from rabbits. The purpose of this study is to introduce some characteristics of IFS. This scaffold was characterized using various techniques, including Masson's trichrome staining, scanning electron microscopy, porosity measurements, and cell counting. The sustained release of growth factors, including PDGF, VEGF, TGF-β1, IGF, FGF, and EGF, was quantified using ELISA assay. The obtained IFS was tested for its effects on cell proliferation, extracellular matrix deposition, and full-thickness skin defect repair. The prepared IFS is characterized by a loose fibrin network structure with white blood cells and platelets that slowly release growth factors and can promote the healing of skin defects via the promotion of cell proliferation, collagen deposition, and tissue revascularization. In addition, its liquid properties and porous structure are conducive to its application as a therapeutic component in tissue engineering. [ABSTRACT FROM AUTHOR]

Lateral intra-articular varus osteotomy is an L-shaped osteotomy of the lateral tibial condyle to correct mild knee valgus and lateral plateau malunion of the proximal tibia. In order to minimize injury, it was modified to preserve the posterolateral cortex and the upper tibiofibular joint. The aim of this study was to evaluate the stability of modified lateral intra-articular varus osteotomy by comparing the biomechanical strength with lateral intra-articular varus osteotomy. Twenty-four synthetic tibia models were divided into 2 groups based on osteotomy type. Each model was then fixed with 2 commonly used plate systems. Biomechanical tests were conducted to measure parameters including construct stiffness, wedge displacement, and the number of failed specimens, and the results were compared among different groups. No significant difference was found in construct stiffness among all groups (P > 0.05). There was also no significant difference in wedge displacement among all groups (P > 0.05). When an axial load of 1500 N was applied, the number of failed specimens showed no significant difference among all groups (P > 0.05). The main failure pattern was additional fracture lines on the lateral tibial plateau. The findings indicate that there was no significant difference in stability between the 2 groups under the tested loading conditions. Furthermore, it appears that preserving the posterolateral cortex may have no impact on biomechanical stability. • Biomechanical study of lateral high tibia osteotomy with synthetic bones. • Modified lateral intra-articular varus osteotomy construct is stable. • Modified osteotomy has comparable reliability compared to traditional osteotomy. [ABSTRACT FROM AUTHOR]

Background: The association between tibial plateau fracture morphology and injury force mechanism has not been well described. The aim of this study was to characterize 3-dimensional fracture patterns associated with hypothesized injury force mechanisms.Methods: Tibial plateau fractures treated in a large trauma center were retrospectively reviewed. Three experienced surgeons divided fractures independently into 6 groups associated with injury force mechanisms proposed from an analysis of computed tomographic (CT) imaging: flexion varus, extension varus, hyperextension varus, flexion valgus, extension valgus, and hyperextension valgus. The fracture lines and comminution zones of each fracture were graphically superimposed onto a 3-dimensional template of the proximal part of the tibia. Fracture characteristics were then summarized on the basis of the fracture maps. The association between injury force mechanism and ligament avulsions was calculated.Results: In total, 353 tibial plateau fractures were included. The flexion varus type pattern was seen in 67 fractures characterized by a primary fracture apex located posteromedially and was frequently associated with concomitant anterior cruciate ligament (ACL) avulsion (44.8%). The extension varus pattern was noted in 60 fractures with a characteristic medial fragment apex at the posteromedial crest or multiple apices symmetrically around the crest and was commonly completely articular in nature (65%). The hyperextension varus pattern was seen in 47 fractures as noted by anteromedial articular impaction, 51% with a fibular avulsion and 60% with posterior tension failure fragments. The flexion valgus pattern was observed in 51 fractures characterized by articular depression posterolaterally, often (58.9%) with severe comminution of the posterolateral cortical rim. The extension valgus patterns in 116 fractures only involved the lateral plateau, with central articular depression and/or a pure split. The hyperextension valgus pattern occurred in 12 fractures denoted by anterolateral articular depression. A moderate positive association was found between flexion varus fractures and ACL avulsions and between hyperextension varus fractures and fibular avulsions.Conclusions: Tibial plateau fractures demonstrate distinct, mechanism-associated 3-dimensional pattern characteristics. Further research is needed to validate the classification reliability among other surgeons and to determine the potential value in the diagnosis and formulation of surgical protocols. [ABSTRACT FROM AUTHOR]

Model identification is divided into two parts: structure identification and parameter identification, and the parameter identification is actually an optimization process. For improving the optimization performance, in this paper, we firstly present a novel conjugate gradient descent method with a modified Armijo-type line search technique to train a Takagi-Sugeno fuzzy neural network model. Numerical simulations are implemented to demonstrate the efficiency of the proposed algorithm. According to the experimental comparisons that are evaluated over 15 classification and 3 regression problems, the advantages of the given method are superior to its another two counterparts. To complement the simulation results and help in establishing a robust fuzzy neural network model, we strictly prove two deterministic convergent behaviors of the presented algorithm, i.e., weak and strong convergence results. They indicate the gradient of the target function with respect to network weights converges to zero and the parameter sequence approaches a fixed optimal point, respectively. [ABSTRACT FROM AUTHOR]

Purpose: Varus deformity of knee osteoarthritis was formed by both intra-articular and extra-articular pathologies. Such intra-articular deformities could not be fully corrected by a medial open-wedge high tibial osteotomy (HTO), which was performed as an extra-articular procedure. Therefore, the purpose of this study was to investigate whether any residual varus was left inside the joint after HTO in the patients with knee osteoarthritis, and a correlation of the residual varus could be traced.Methods: This study involved 66 patients (66 knees) undergoing HTO for medial knee osteoarthritis. The percentage of mechanical axis (%MA), mechanical femorotibial angle (mFTA), mechanical lateral distal femoral angle (mLDFA), mechanical medial proximal tibial angle (mMPTA) and joint line convergence angle (JLCA) were measured on radiographs of the full-length legs preoperatively and 6 months postoperatively. The relationship between changes in the JLCA and alignment correction was assessed. The postoperative residual JLCA was categorized as the optimal (postoperative JLCA ≤ 2°), the acceptable (2° < postoperative JLCA ≤ 5°), and the unacceptable (postoperative JLCA > 5°) to analyze its correlation with pre- or intra-operative factors.Results: Average %MA and mFTA were improved from 5.5 to 60% and from 190.2° to 176.4°, respectively. There was no change in mLDFA, whereas mMPTA changed from 80.3° to 91.8°. JLCA changed from 4.2° to 2.7°. The analyses of multiple linear regression showed that the preoperative JLCA and postoperative changes in mechanical alignment (%MA, mFTA and mMPTA) were two important variables dependently associated with differences in JLCAs postoperatively. However, postoperative JLCAs showed a stronger correlation to preoperative JLCAs than to changes in mechanical alignment postoperatively. A Chi-square analysis showed a significantly higher percentage of patients achieved acceptable postoperative JLCAs in the preoperative JLCA ≤ 6° group (78.8%) compared to the preoperative JLCA > 6° group (6.1%). Therefore, 6° of JLCA was suggested to be a tipping point.Conclusions: The capability of HTO to correct intra-articular varus deformities, which was represented by JLCAs, is limited. Postoperative residual JLCAs were correlated primarily to preoperative JLCA values and total alignment correction, while the former accounted for most. A preoperative JLCA of 6° was suggested to be a tipping point, and a larger value indicated more than 5° residual JLCA after the HTO. [ABSTRACT FROM AUTHOR]

Background. Platelet-rich plasma (PRP) has been shown to be a promising therapeutic agent against osteoarthritis (OA), whereas its chondroprotection mechanism is not fully elucidated. Autophagy is considered an important biological process throughout the development of OA. Therefore, the objective of the present study is to investigate the role of autophagy in the chondroprotection and compare the effects of releasate between L-PRP and P-PRP. Methods. PRP were prepared from rat blood. Rat chondrocytes pretreated in the presence or absence of interleukin-1 beta (IL-1β) were incubated with PRP releasate. The expressions of OA-related genes and autophagy-related genes were determined by RT-PCR and western blot, respectively. Autophagic bodies were assessed by transmission electron microscopy and the autophagy flux was monitored under the confocal microscopy. The effect of PRP on autophagy was further investigated in the milieu of autophagy activator, rapamycin, or autophagy inhibition by downregulation of Atg5. The effect of PRP on cartilage repair and autophagy was also evaluated in an OA rat model. Results. In vitro, PRP releasate increased the expression of the anabolic genes, COL2 and Aggrecan, and decreased the expression of the catabolic genes, whereas the expression of autophage markers, Atg5 and Beclin-1, as well as the ratio of LC3 II/LC3 I, was not significantly altered in normal or IL-1β-treated chondrocytes. Similar expression pattern was found following the activation (rapamycin) or inhibition (Atg5 silencing) of autophagy. In vivo, PRP releasate ameliorated posttraumatic cartilage degeneration while the expression of LC3 was comparable to that in the vehicle treatment group. Conclusions. PRP releasate promoted the anabolic gene expression, relieved inflammatory stress in chondrocytes, and ameliorated cartilage degeneration, but autophagy was independent of these processes. [ABSTRACT FROM AUTHOR]

Background: Hoffa fractures, coronal-plane fractures involving the distal femoral condyles, are unstable, intra-articular fractures. The aim of this study was to define the location and frequency of fracture lines and comminution zones in Hoffa fractures using computed tomography (CT) mapping in both 2-dimensional and 3-dimensional contexts.Methods: Seventy-five Hoffa fractures (OTA/AO types 33B3.2 and 33B3.3) were retrospectively reviewed. The directions of fracture lines were characterized in the axial and sagittal CT planes. CT images for all fractures were superimposed on one another and oriented to fit a standard template. Mapping of fracture lines and comminution zones in both the axial and sagittal planes was performed. A 3-dimensional map was created by reducing reconstructed fracture fragments to fit to a model of the distal aspect of the femur.Results: This study included 1 bicondylar and 74 unicondylar (26 medial and 48 lateral) Hoffa fractures. Comminuted fractures accounted for 35.5% of all fractures and 44.9% of lateral fractures. Axial fracture mapping demonstrated that fracture lines were concentrated in the middle-third area of the lateral condyle but were less concentrated and with greater variation in the medial condyle. The mean angle of fracture lines with respect to the posterior condylar axis was 34.4° and 29.0° in the lateral and medial femoral condyles, respectively. Sagittal fracture mapping also demonstrated that fracture lines were concentrated in the middle third of the lateral condyle but were less concentrated in the medial condyle. The mean angle of fracture lines with respect to the posterior cortex of the distal femoral shaft was 23.1° and 19.3° in the lateral and medial condyles, respectively. Three-dimensional mapping demonstrated comminution zones commonly occurring in the weight-bearing zone of the lateral condylar articular surface.Conclusions: Hoffa fractures occurred more frequently in the lateral femoral condyle. In the axial plane, fractures commonly extended from anterolateral to posteromedial in the lateral condyle and from anteromedial to posterolateral in the medial femoral condyle. In the sagittal plane, fractures traversed from anteroinferior to posterosuperior. Articular comminution was more commonly seen in lateral condylar fractures and concentrated in the weight-bearing zone of the articular surface.Clinical Relevance: Research in this area is imperative for optimal preoperative planning, such as for the selection of surgical approach and fixation constructs. Our findings lend insight into fracture morphology, which can assist with fracture classification and the design of biomechanical studies, ultimately aiding in treatment. [ABSTRACT FROM AUTHOR]

Background: High levels of pro-inflammatory cytokines in leukocyte- and platelet-rich plasma (L-PRP) may activate the nuclear factor κB (NF-κB) pathway to counter the beneficial effect of the growth factors on bone regeneration. However, to date, no relevant studies have substantiated this.Methods: L-PRP and pure platelet-rich plasma (P-PRP) were isolated. The in vitro effects of L-PRP and P-PRP on the proliferation, viability and migration of human bone marrow-derived mesenchymal stem cells (HBMSCs) and EaHy926, tube formation of EaHy926, and osteogenic differentiation of HBMSCs were assessed by cell counting, flow cytometry, scratch assay, tube formation assay, and real-time quantitative polymerase chain reaction (RT-PCR), western blotting and Alizarin red staining, respectively. The in vitro effects of L-PRP and P-PRP on the nuclear translocation of NF-κB p65, mRNA expression of inducible nitric oxide synthase and cyclooxygenase-2, and production of prostaglandin E2 and nitric oxid were assessed by western blotting, RT-PCR, enzyme-linked immunosorbent assay and Griess reaction, respectively. The in vivo effects of L-PRP or P-PRP preprocessed β-tricalcium phosphate (β-TCP) on the calvarial defects in rats were assessed by histological and immunofluorescence examinations.Results: P-PRP, which had similar platelet and growth factors concentrations but significantly lower concentrations of leukocytes and pro-inflammatory cytokines compared with L-PRP, promoted the proliferation, viability and migration of HBMSCs and EaHy926, tube formation of EaHy926 and osteogenic differentiation of HBMSCs in vitro, compared with L-PRP. The implantation of P-PRP preprocessed β-TCP also yielded better histological results than the implantation of L-PRP preprocessed β-TCP in vivo. Moreover, L-PRP treatment resulted in the activation of the NF-κB pathway in HBMSCs and EaHy926 in vitro while the postoperative delivery of caffeic acid phenethyl ester, an inhibitor of NF-κB activation, enhanced the histological results of the implantation of L-PRP preprocessed β-TCP in vivo.Conclusions: Leukocytes in L-PRP may activate the NF-κB pathway via the increased pro-inflammatory cytokines to induce the inferior effects on bone regeneration of L-PRP compared with P-PRP. Hence, P-PRP may be more suitable for bone regeneration compared with L-PRP, and the combined use of P-PRP and β-TCP represents a safe, simple, and effective alternative option for autogenous bone graft in the treatment of bone defects. [ABSTRACT FROM AUTHOR]

Background: Traditionally, acute undisplaced or minimally-displaced scaphoid fractures are treated by casting in short- or long-arm casts. Although reports have shown that operative treatment is safe, effective and produces satisfactory results, outcomes from current studies comparing these two methods are questionable. The aim of this meta-analysis was to evaluate the effects of operative versus non-operative treatment for acute undisplaced or minimally-displaced scaphoid fractures in adults. Methods: Computerized searches were performed without language restrictions and all randomized controlled studies providing information on the effects of operative versus non-operative treatment on the outcomes of acute undisplaced or minimally-displaced scaphoid fractures were included. The weighted and standard mean difference (WMD and SMD) or the relative risk (RR) were calculated for continuous or dichotomous data respectively. Results: A total of six studies reported in seven publications were included, representing data on 340 fractures. Meta-analysis indicated that operative treatment resulted in significantly better functional outcomes in the short term when compared with non-operative treatment. Consistently, patients who accepted surgery had a more rapid return to work. Further, surgery was advantageous in preventing delayed union of the fractures, a finding supported by the results of analysis of the time to fracture union. A number-needed-to-treat analysis revealed that more than 20 patients would have to undergo operative treatment to prevent one delayed union. Conclusion: Acute undisplaced or minimally-displaced scaphoid fractures demonstrate faster recovery with operative treatment; however, the current meta-analysis does not provide evidence supporting the routine use of operative treatment for all acute undisplaced or minimally-displaced scaphoid fractures. [ABSTRACT FROM AUTHOR]

Lateral hinge fractures are common complications in the medial opening wedge high tibial osteotomy for treatment of knee osteoarthritis. The rehabilitation protocols are decided depending on the remaining stability following these fractures. This study aimed to evaluate the biomechanical properties of different types of lateral hinge fractures in medial opening wedge high tibial osteotomy. Twenty synthetic tibia models were used as test samples. A 10-mm bone wedge was removed from the medial side of the proximal tibias to create the bone defect. The samples were then divided into 4 groups: (1) intact lateral hinge; (2) Takeuchi type I fractures; (3) type II fractures; and (4) type III fractures. After fixation with a locking plate, the stability parameters including construct stiffness, wedge displacement, and construct strength were tested under compressive forces and compared among the 4 groups. No statistical difference was found in the construct stiffness among the 4 groups (P = 0.78). The type III fractures had the largest wedge displacement compared with the other 3 groups. The failure loads on average were significantly reduced in the type III fractures compared with those with intact hinge (P < 0.01) and in type I fractures (P = 0.04). No statistical difference was observed between the type I fractures and the intact hinge in terms of wedge displacement or failure loads. The type III fractures were the most unstable and patients with these fractures should be managed cautiously. Delayed weightbearing and/or additional fixation should be considered. • The first biomechanical study of lateral hinge fractures in high tibial osteotomy. • Takeuchi Type I fractures are the most stable lateral hinge fractures. • Takeuchi Type III fractures are the least stable lateral hinge fractures. • Additional fixation intraoperatively should be considered in type III fractures. • Delayed weightbearing postoperatively is recommended for type III fractures. [ABSTRACT FROM AUTHOR]