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Your search keyword '"Xie, Yinshuai"' showing total 6 results
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6 results on '"Xie, Yinshuai"'

4. Identification of individual plants carrying viruses or a viroid in Pyrus betulifoliaseedlings and virus-tested pear mother plants by group testing

Author

Jiang, Feng, Li, Hanwei, Xie, Yinshuai, Wang, Jinying, Chi, Hai, Kan, Qing, Yu, Shangzhen, Li, Tianzhong, and Cheng, Yuqin

Abstract

Monitoring the sanitary status of a large population of vegetative propagation materials is a challenge because it requires expensive and time-consuming testing. Here we report a group testing strategy to identify individual plants carrying viruses and/or a viroid in Pyrus betulifoliaseedlings and virus-tested pear mother plants. First, the effect of pool size on RT-PCR detection of apple stem grooving virus (ASGV), apple stem pitting virus (ASPV), apple chlorotic leaf spot virus (ACLSV) and apple scar skin viroid (ASSVd) was evaluated; all of them could be sensitively detected in a bulk (pool) of eight samples. The virological condition of 2770 P. betulifoliaseedlings and 110 virus-tested pear mother plants were then investigated by RT-PCR using a grouping strategy with 5 samples. We identified 20, 35 and 3 P. betulifoliaseedlings as positive for ASGV, ASPV, and ASSVd, respectively, and 4 pear mother plants were identified as carrying ASGV. Our data indicated that RT-PCR testing on samples pooled before RNA extraction is a reliable and resource-efficient strategy to monitor the sanitary status of large numbers of pear vegetative propagation materials. The results presented here also highlight the possibility of pear seedling rootstocks being a source of viral infection.

Published
2024
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5. The phenylcoumaran benzylic ether reductase gene PtPCBER improves the salt tolerance of transgenic poplar through lignan-mediated reactive oxygen species scavenging

Author

Wei, Mingxing, primary, Yu, Chunyan, additional, Ge, Bingkun, additional, Liu, Yue, additional, Zhang, Haiyang, additional, Duan, Chunli, additional, Zhang, Juan, additional, Mao, Tingting, additional, Huang, Huiqing, additional, Xie, Yinshuai, additional, Gao, Hongsheng, additional, Zhang, Hongxia, additional, Wang, Aike, additional, and Yi, Yanjun, additional

Published
2022
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6. GLRaV-2 protein p24 suppresses host defenses by interaction with a RAV transcription factor from grapevine.

Author

Zhang C, Wang X, Li H, Wang J, Zeng Q, Huang W, Huang H, Xie Y, Yu S, Kan Q, Wang Q, and Cheng Y

Subjects
Plant Diseases genetics, RNA Interference, Transcription Factors genetics, Transcription Factors metabolism, Closterovirus genetics, Closterovirus metabolism, Viral Proteins metabolism, Vitis genetics, Vitis metabolism
Abstract

Grapevine leafroll-associated virus 2 (GLRaV-2) is a prevalent virus associated with grapevine leafroll disease, but the molecular mechanism underlying GLRaV-2 infection is largely unclear. Here, we report that 24-kDa protein (p24), an RNA-silencing suppressor (RSS) encoded by GLRaV-2, promotes GLRaV-2 accumulation via interaction with the B3 DNA-binding domain of grapevine (Vitis vinifera) RELATED TO ABSCISIC ACID INSENSITIVE3/VIVIPAROUS1 (VvRAV1), a transcription factor belonging to the APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) superfamily. Salicylic acid-inducible VvRAV1 positively regulates the grapevine pathogenesis-related protein 1 (VvPR1) gene by directly binding its promoter, indicating that VvRAV1 may function in the regulation of host basal defense responses. p24 hijacks VvRAV1 to the cytoplasm and employs the protein to sequester 21-nt double-stranded siRNA together, thereby enhancing its own RSS activity. Moreover, p24 enters the nucleus via interaction with VvRAV1 and weakens the latter's binding affinity to the VvPR1 promoter, leading to decreased expression of VvPR1. Our results provide a mechanism by which a viral RSS interferes with both the antiviral RNA silencing and the AP2/ERF-mediated defense responses via the targeting of one specific host factor., (© American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com.)

Published
2022
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