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A new Three-Dimensional (3D) scene reconstruction method is proposed to meet the practical application requirement of the active cylindrical scanning millimeter-wave 3D imaging security apparatus. Specifically, the w-K algorithm is firstly used to realize the decoupling and focusing between the antenna array direction and the range direction, then the synthetic aperture processing between the distance and angle direction is performed to achieve the focusing with the Back Projection (BP) algorithm, realizing the fully 3D scene reconstruction. The echo data processing results of the simulated and measured 3D human model show that this method is theoretical feasible with good practical applicability. Besides, the proposed method could realize the fast and accurate 3D human imaging by CUDA platform and be applicable to the nonideal cylindrical scanning 3D imaging scenarios.

Abstract The description of a single sand body for remaining oil predictions is critical to the enhancement of oil recovery of an old oilfield. Taking the fluvial facies of the Xingbei Oilfield as an example, a single sand body can be divided into four categories—“tabulated reservoir”, “untabulated reservoir”, “single channel sand body” and “abandoned channel”—using the reservoir architecture analysis method. The boundary surface of each type may be mud barriers or only an erosion surface, which was traced by careful anatomization of the single sand body. Then, a fine single sand body reservoir geological model was constructed using a combination of a determined modelling method and stochastic modelling method. The numerical simulation is executed using the constructed geological model to forecast the remaining oil distribution quantitatively. The results show that the remaining oil was distributed in the bottom parts of the abandoned channel, top part of the point bar, tabulated reservoir, and channel edges. The movements of the injection water were mainly controlled by the mud barrier and superimposed styles of single sand body, which determines the formation of the remaining oil. This research has important guidance for oilfield development in the late stage, whose reservoir is composed of single sand bodies.

Abstract Background Streptomyces species are widely distributed in natural habitats, such as soils, lakes, plants and some extreme environments. Replication loci of several Streptomyces theta-type plasmids have been reported, but are not characterized in details. Conjugation loci of some Streptomyces rolling-circle-type plasmids are identified and mechanism of conjugal transferring are described. Results We report the detection of a widely distributed Streptomyces strain Y27 and its indigenous plasmid pWTY27 from fourteen plants and four soil samples cross China by both culturing and nonculturing methods. The complete nucleotide sequence of pWTY27 consisted of 14,288 bp. A basic locus for plasmid replication comprised repAB genes and an adjacent iteron sequence, to a long inverted-repeat (ca. 105 bp) of which the RepA protein bound specifically in vitro, suggesting that RepA may recognize a second structure (e.g. a long stem-loop) of the iteron DNA. A plasmid containing the locus propagated in linear mode when the telomeres of a linear plasmid were attached, indicating a bi-directional replication mode for pWTY27. As for rolling-circle plasmids, a single traA gene and a clt sequence (covering 16 bp within traA and its adjacent 159 bp) on pWTY27 were required for plasmid transfer. TraA recognized and bound specifically to the two regions of the clt sequence, one containing all the four DC1 of 7 bp (TGACACC) and one DC2 (CCCGCCC) and most of IC1, and another covering two DC2 and part of IC1, suggesting formation of a high-ordered DNA-protein complex. Conclusions This work (i) isolates a widespread Streptomyces strain Y27 and sequences its indigenous theta-type plasmid pWTY27; (ii) identifies the replication and conjugation loci of pWTY27 and; (iii) characterizes the binding sequences of the RepA and TraA proteins.

Abstract Background Streptomyces coelicolor is the most studied Streptomyces species and an excellent model for studying differentiation and antibiotic production. To date, many genes have been identified to be required for its differentiation (e.g. bld genes for aerial growth and whi genes for sporulation) and antibiotics production (including actII-orf4, redD, cdaR as pathway-specific regulatory genes and afsR, absA1/A2 as pleiotropic regulatory genes). Results A gene cluster containing six genes (SCO4126-4131) was proved to be co-transcribed in S. coelicolor. Deletions of cmdABCDEF (SCO4126-4131) displayed defective sporulation including formation of aberrant branches, and abnormalities in chromosome segregation and spore septation. Disruption mutants of apparently orthologous genes of S. lividans and S. avermitilis also showed defective sporulation, implying that the role of these genes is similar among Streptomyces. Transcription of cmdB, and therefore presumably of the whole operon, was regulated developmentally. Five of the encoded proteins (CmdA, C, D, E, F) were predicted membrane proteins. The other, CmdB, a predicted ATP/GTP-binding protein with an ABC-transporter-ATPase domain shown here to be essential for its function, was also located on the cell membrane. These results indicate that CmdABCDEF proteins mainly affect Streptomyces differentiation at an early stage of aerial hyphae formation, and suggest that these proteins may form a complex on cell membrane for proper segregation of chromosomes. In addition, deletions of cmdABCDEF also revealed over-production of blue-pigmented actinorhodin (Act) via activation of transcription of the pathway-specific regulatory gene actII-orf4 of actinorhodin biosynthesis. Conclusion In this study, six co-transcribed genes cmdABCDEF were identified by their effects on differentiation and antibiotic production in Streptomyces coelicolor A3(2). These six membrane-located proteins are possibly assembled into a complex to function.