Search

Heterostructured design and microcrack management mitigate the strength-ductility dilemma in metallic materials. Here, we demonstrate a simultaneous enhancement of strength and ductility in graphene-decorated bimodal pure nickel, achieving a strength increase to 1 GPa and an 18% improvement in elongation by designing heterostructure with graphene, metal powders, and metal foams. The results show that grain refinement, hetero-deformation-induced hardening, and activated stacking faults mainly cause the strength-ductility synergy. Additionally, while dispersed microcracks nucleate within the fine grain zones, their propagation is constrained by the coarse grain zones. This study provides new insights into improving the strength-ductility combination in metallic materials.

Abstract Prostate cancer is one of the most common cancers in men and poses a significant threat to global male health. Traditional prostate cancer assessment methods have certain limitations, necessitating the identification of new prognostic factors and treatment targets. Our study revealed that low expression of the cornichon family AMPA receptor auxiliary protein 2 (CNIH2) gene was associated with a better progression-free survival rate in prostate cancer patients. The area under the receiver operating characteristic (ROC) curve (AUC) showed that the prognostic ability of the CNIH2 gene was high at 1, 3, and 5 years. The gene was an independent prognostic factor according to multivariate analysis. Functional verification experiments showed that knocking down the CNIH2 gene could inhibit the proliferation, migration and invasion of prostate cancer cells and could also inhibit tumor growth in nude mice. Our study is the first to reveal the important role of the CNIH2 gene in prostate cancer. This discovery provides a new research direction for individualized treatment and prognostic evaluation of prostate cancer.

Abstract Inflammation plays a crucial role in cancer development. The neutrophil-to-lymphocyte ratio (NLR), a measure of inflammation, is obtained from a complete blood count. However, little is known about the association between NLR and cancer in the general adult population in the United States. This study aimed to evaluate whether NLR is associated with cancer in American adults. This retrospective cross-sectional study included 28,016 adult participants from the National Health and Nutrition Examination Survey (NHANES) dataset spanning 2005 to 2018. Data on demographics (age, sex, race, marital status, Poverty-Income Ratio, education level), lifestyle factors (smoking, alcohol consumption, body mass index), medical conditions (hypertension, diabetes, cardiovascular disease), and laboratory parameters (hemoglobin, platelet count, alanine aminotransferase, creatinine, albumin, and lactate dehydrogenase), were collected. Logistic regression analysis was used to investigate the research objectives. Of the total 28,016 participants, 2639 had cancer. The mean age was 49.6 ± 17.6 years, and 50% were male. A positive association between NLR and cancer risk was observed after multivariate adjustment (OR = 1.20, 95% confidence interval (CI) = 1.05–1.36, p = 0.006). Similar patterns were observed in subgroup analyses (all p-values for interaction > 0.05). A higher NLR was directly correlated with an increased risk of developing cancer in adults.

Abstract Coherent dispersive wave emission, as an important phenomenon of soliton dynamics, manifests itself in multiple platforms of nonlinear optics from fibre waveguides to integrated photonics. Limited by its resonance nature, efficient generation of coherent dispersive wave with ultra-broad bandwidth has, however, proved difficult to realize. Here, we unveil a new regime of soliton dynamics in which the dispersive wave emission process strongly couples with the splitting dynamics of the driving pulse. High-order dispersion and self-steepening effects, accumulated over soliton self-compression, break the system symmetry, giving rise to high-efficiency generation of coherent dispersive wave in the ultraviolet region. Simultaneously, asymmetric soliton splitting results in the appearance of a temporally-delayed ultrashort pulse with high intensity, overlapping and copropagating with the dispersive wave pulse. Intense cross-phase modulations lead to octave-wide broadening of the dispersive wave spectrum, covering 200–400 nm wavelengths. The highly-coherent, octave-wide ultraviolet spectrum, generated from the simple capillary fibre set-up, is in great demand for time-resolved spectroscopy, ultrafast electron microscopy and frequency metrology applications, and the critical role of the secondary pulse in this process reveals some new opportunities for all-optical control of versatile soliton dynamics.

The rapid development of informatization and digitalization in public security business has generated a large amount of law enforcement case data in public security work. However, due to various types of text and large amount of information, front-line police officers often face problems such as low reading efficiency and difficulty in aggregating information in the process of reading case files. In order to further utilize the law enforcement case text, it is necessary to conduct intelligent analysis and knowledge extraction. However, due to the professionalism, data sensitivity, confidentiality of public security professional law enforcement case text, as well as the requirements of public security data going out of the network, only a small number of learning training samples can be obtained, and the traditional deep learning model has unsatisfactory extraction effect. Therefore, this paper proposes to build a large language model in vertical fields with fewer resources and data, and realize the adaptation of the model to the public security profession. The model uses knowledge editing technology MEMIT (mess-editing memory in a transformer), low-resource fine-tuning technology LoRA (low-rank adaptation), and prompt templates to improve the model??s understanding of public security knowledge such as police terminology and common sense. Moreover, in order to further improve the knowledge extraction effect of the model, a small sample law enforcement case text data extraction process is designed to better integrate the professional knowledge related to the case in the model. Experimental results show that the accuracy of the public security professional vertical field large language model integrated with the extraction process in various knowledge extraction tasks is significantly improved compared with the traditional methods, which helps front-line police officers quickly, objectively and accurately analyze law enforcement case text, dig out potential case information, and support the intelligent development of public security work.

Abstract This study aimed to design a VEGFR-targeting peptide–drug conjugate with the ability to decrease tumor burden and suppress tumor angiogenesis, and to further evaluate the therapeutic effect of anti-PD-1 antibody in HCC therapy. A VEGFR-targeting peptide VEGF125 − 136 (QR) was conjugated with a lytic peptide (KLU) to form a peptide–drug conjugate QR-KLU. And the efficacy of QR-KLU in combination with anti-PD-1 antibody for HCC therapy in vivo and in vitro were evaluated. QR-KLU inhibited the proliferation and migration of mouse HCC cell line (Hepa1–6) cells under normoxic and hypoxic conditions in a dose-dependent manner. In the subcutaneous Hepa1–6 tumor model, QR-KLU combined with the anti-PD-1 antibody substantially inhibited tumor growth, promoted tumor necrosis, and prolonged the survival time of tumor-bearing mice. QR-KLU substantially inhibited hypoxia-induced expression of VEGF, promoted tumor vascular normalization, and increased cluster of differentiation 8+ (CD8+) T cell infiltration in the tumor. In addition, QR-KLU and anti-PD-1 antibody demonstrated a strong synergistic effect in promoting the activation of intratumoral CD8+ T cells, reducing the expression of immune-inhibitory factors, and increasing the expression of immune-stimulatory factors. This study proposed a novel approach for enhancing the efficacy of anti-PD-1 antibody using a VEGFR-targeting peptide–drug conjugate in HCC therapy.

Abstract Low-cost and green preparation of efficient sorbents is critical to the removal of organic contaminants during water treatment. In this study, the co-pyrolysis of macroalgae and oyster shell was designed to synthesize nitrogen-doped porous biochars for sorption removal of atrazine from water. Oyster shell played a significant role in opening pores in macroalgae-derived biochars, resulting in the surface area of the macroalgae (Enteromorpha prolifera and Ulva lactuca) and oyster shell co-pyrolyzed carbonaceous as high as 1501.80 m2 g−1 and 1067.18 m2 g−1, the pore volume reached 1.04 cm3 g−1 and 0.93 cm3 g−1, and O/C decreased to 0.09 and 0.08, respectively. The sorption capacity of atrazine to nitrogen-doped porous biochars (the Enteromorpha prolifera, Ulva lactuca and oyster shell co-pyrolyzed carbonaceous) reached 312.06 mg g−1 and 340.52 mg g−1. Pore-filling, hydrogen bonding, π-π or p-π stacking and electrostatic interaction dominated the multilayer sorption process. Moreover, the nitrogen-doped porous biochars showed great performance in cyclic reusability, and the Enteromorpha prolifera, Ulva lactuca and oyster shell co-pyrolyzed carbonaceous sorption capacity still reached 246.13 mg g−1 and 255.97 mg g−1, respectively. Thus, this study suggested that it is feasible and efficient to remove organic contaminants with the nitrogen-doped porous biochars co-pyrolyzed from macroalgae and oyster shell, providing a potential green resource utilization of aquatic wastes for environmental remediation. Graphical Abstract

Purpose: The purpose of this study is to reveal the influence mechanism of social innovation education (SIE) on sustainability learning outcomes and analyze the roles of intrinsic learning motivation and prosocial motivation. Design/methodology/approach: A sample of 322 undergraduates from one higher education institution in Tianjin was used to test the hypotheses. Findings: This study found that SIE positively affected sustainability learning outcomes, and intrinsic learning motivation mediated the relationship between them. The results showed that prosocial motivation positively moderates the positive effect of SIE on intrinsic learning motivation and the overall mediation model. Practical implications: The findings have important practical implications for higher education institutions to carry out SIE. Higher education institutions should focus on integrating social innovation and sustainability into top-level design. Furthermore, higher education institutions should focus on stimulating students' intrinsic learning motivation and cultivating their prosocial motivation. Originality/value: This study identified the relationship between SIE and sustainability learning outcomes and clarified the influence mechanism of SIE on sustainability learning outcomes. Moreover, this study emphasized the importance of prosocial motivation as a key boundary condition of SIE.

The age at which people acquire a word influences word recognition, known as the age of acquisition (AoA) effect. In the first language (L1), AoA effects are widely found in various languages and experimental tasks. Arbitrary Mapping Hypothesis proposes that AoA effects reflect the loss of network plasticity during the learning of mappings between input and output representations. It predicts that the AoA effect appears (or is larger) when the input-output mapping is arbitrary/inconsistent, relative to consistent input-output mapping. The present study examined how these predictions generalised to the second language (L2). We explored whether the L2 AoA effect was modulated by spelling-sound consistency in Chinese-English bilinguals, adopting a delayed word naming task. The results showed that the mapping consistency modulated the L2 AoA effect on the N170, P200 and N400 components. L2 AoA effect was insignificant in the consistent condition but was significant in the inconsistent condition: late-acquired words elicited larger N170, smaller P200, and larger N400 compared to early-acquired words. These findings suggest that L2 AoA effects occur in the spelling-sound connections, providing direct evidence for the Arbitrary Mapping Hypothesis.

The application of machine learning techniques in biological research, especially when dealing with limited data availability, poses significant challenges. In this study, we leveraged advancements in method development for predicting protein-protein binding strength to conduct a systematic investigation into the application of machine learning on limited data. The binding strength, quantitatively measured as binding affinity, is vital for understanding the processes of recognition, association, and dysfunction that occur within protein complexes. By incorporating transfer learning, integrating domain knowledge, and employing both deep learning and traditional machine learning algorithms, we mitigated the impact of data limitations and made significant advancements in predicting protein-protein binding affinity. In particular, we developed over 20 models, ultimately selecting three representative best-performing ones that belong to distinct categories. The first model is structure-based, consisting of a random forest regression and thirteen handcrafted features. The second model is sequence-based, employing an architecture that combines transferred embedding features with a multilayer perceptron. Finally, we created an ensemble model by averaging the predictions of the two aforementioned models. The comparison with other predictors on three independent datasets confirms the significant improvements achieved by our models in predicting protein-protein binding affinity. The programs for running these three models are available at https://github.com/minghuilab/BindPPI.

ObjectiveTo understand the pneumococcal vaccination status and its influencing factors among young and middle-aged populations, and provide reference for promoting pneumococcal vaccination. MethodsFrom May to August 2023, a multi-stage random cluster sampling method was used to select 5 928 residents aged 19 – 59 years old from Nanchang city in Jiangxi province, Changsha city in Hunan province, and Kunming city in Yunnan province for an online questionnaire survey on pneumococcal vaccination status. ResultsAmong the 4 895 young and middle-aged people finally included in the analysis, only 833 had received pneumococcal vaccine in the past 5 years, with a pneumococcal vaccine uptake rate of 17.02%; multivariate unconditional logistic regression analysis showed that the pneumococcal vaccine uptake rates were higher among those aged 45 – 59 years old, agriculture, forestry, animal husbandry and fishery production personnel, professional and technical personnel, smokers, those with general knowledge of pneumococcal diseases and vaccines, and those with general and good trust in pneumococcal vaccines, while the uptake rates were lower among those with high school/technical secondary school education or above and office clerks and related personnel; the top three reasons for pneumococcal vaccination among young and middle-aged people were disease prevention (75.87%), protecting family and colleagues (57.38%), and government and health administration recommending vaccination (43.22%), while the top three reasons for not receiving pneumococcal vaccine were worrying about adverse reactions after vaccination (42.22%), no time for vaccination (28.19%), and believing that the vaccine had limited effectiveness (25.11%), and the top three factors most likely to change vaccination willingness were free/discounted vaccines (46.63%), worrying about the impact of pneumonia infection on family and colleagues (46.53%), and government and health administration recommending vaccination (45.89%). ConclusionThe pneumococcal vaccine uptake rate among young and middle-aged populations was low, and age, education level, occupation, smoking status, knowledge of pneumococcal diseases and vaccines, and trust in vaccines was the main influencing factors for pneumococcal vaccination in this population. Comprehensive policy measures, especially the introduction of preferential policies related to pneumococcal vaccines, should be adopted to promote vaccination.

Abstract Background Helicobacter pylori (H. pylori) infection is critical in the development and occurrence of gastric cancer. H. pylori secretes gamma-glutamyl transferase (GGT), which affects energy metabolism and histone methylation in mesenchymal stem cells. However, its effect on human gastric epithelial cells remains unclear. This study aimed to investigate the effects of GGT on energy metabolism and histone methylation in gastric epithelial cells and determine its role in the development and progression of H. pylori-induced gastric cancer. Methods A GGT knockout H. pylori strain and mouse gastric cancer model were constructed, and alpha-ketoglutarate (α-KG) was added. The underlying mechanism was investigated using proteomics, immunohistochemistry, Western blotting, and other experimental assays. Results H. pylori can colonize the host’s stomach and destroy the gastric epithelium. GGT secreted by H. pylori decreased the concentration of glutamine in the stomach and increased H3K9me3 and H3K27me3 expression, which promoted the proliferation and migration of gastric epithelial cells. Additionally, α-KG reversed this effect. GGT increased the tumorigenic ability of nude mice. GGT, secreted by H. pylori, promoted the expression of ribosomal protein L15 (RPL15), while GGT knockout and supplementation with α-KG and trimethylation inhibitors reduced RPL15 expression and Wnt signaling pathway expression. Conclusions H. pylori secreted GGT decreased the expression of glutamine and α-KG in gastric epithelial cells, increased the expression of histones H3K9me3 and H3K27me3, and activated the Wnt signaling pathway through RPL15 expression, ultimately changing the biological characteristics of the gastric epithelium and promoting the occurrence of gastric cancer. Altered energy metabolism and histone hypermethylation are important factors involved in this process. Graphical abstract

Abstract The effects of stochastic volatility, jump clustering, and regime switching are considered when pricing variance swaps. This study established a two-stage procedure that simplifies the derivation by first isolating the regime switching from other stochastic sources. Based on this, a novel probabilistic approach was employed, leading to pricing formulas with time-dependent and regime-switching parameters. The formulated solutions were easy to implement and differed from most existing results of variance swap pricing, where Fourier inversion or fast Fourier transform must be performed to obtain the final results, since they are completely analytical without involving integrations. The numerical results indicate that jump clustering and regime switching have a significant influence on variance swap prices.

The CIR stochastic volatility model is modified to introduce nonlinear mean reversion, with the long-run volatility average as a random variable controlled by two parts being modeled through a Brownian motion and a Markov chain, respectively. This model still possesses an analytical formulation of the forward characteristic function, from which we establish variance swap prices as well as volatility swap ones with a nonlinear payoff in closed form. The numerical implementation of the two formulae demonstrates the significant impact of regime switching.

Abstract Background Mobility limitations (e.g., using wheelchair) have been closely linked to diminished functional independence and quality of life in older adults. The regulation of mobility is pertaining to multiple neurophysiologic and sociodemographic factors. We here aimed to characterize the relationships of these factors to the risk of restricted mobility in older adults. Methods In this longitudinal study, 668 older adults with intact mobility at baseline completed the baseline assessments of clinical characteristics, cognitive function, sleep quality, activities of daily living (ADL), walking performance, beat-to-beat blood pressure, and structural MRI of the brain. Then 506 of them (mean age = 70.7 ± 7.5 years) responded to the follow-up interview on the mobility limitation (as defined by if using wheelchair, cane, or walkers, or being disabled and lying on the bed) after 18 ± 3.5 months. Logistic regression analyses were performed to examine the relationships between the baseline characteristics and the follow-up mobility restriction. Results At baseline, compared to intact-mobility group (n = 475), restricted-mobility group (n = 31) were older, with lower score of ADL and the Montreal Cognitive Assessment (MoCA), greater score of Pittsburgh Sleep Quality Index (PSQI), poorer cardio- and cerebral vascular function, and slower walking speeds (ps

Abstract. Triggering receptor expressed on myeloid cells-1 (TREM-1) is a member of the immunoglobulin superfamily. As an amplifier of the inflammatory response, TREM-1 is mainly involved in the production of inflammatory mediators and the regulation of cell survival. TREM-1 has been studied in infectious diseases and more recently in non-infectious disorders. More and more studies have shown that TREM-1 plays an important pathogenic role in kidney diseases. There is evidence that TREM-1 can not only be used as a biomarker for diagnosis of disease but also as a potential therapeutic target to guide the development of novel therapeutic agents for kidney disease. This review summarized molecular biology of TREM-1 and its signaling pathways as well as immune response in the progress of acute kidney injury, renal fibrosis, diabetic nephropathy, immune nephropathy, and renal cell carcinoma.

During the process of electric arc remelting， the content of gas components （such as H， O， N， etc.） in the steel is significantly influenced by the gas permeability of the slag system used. In order to explore a slag system with low hydrogen permeability suitable for nuclear-grade 316H stainless steel in electric arc remelting， the hydrogen permeability of five slag systems used in electric arc remelting was determined. The results showed that the newly developed 63%CaF2-30%Al2O3-7%MgO slag system had the lowest hydrogen permeability.meanwhile the hydrogen permeability of slag system， 65%CaF2-30%Al2O3-5%MgO， was similar to that of the 63%CaF2-30%Al2O3-7%MgO slag system， and hydrogen permeability of both slag systems exhibited a significant reduction compared to the original slag system used in the steel plant's electric arc production，declined from 6.58×10-6 mol/（cm·min） to 1.89×10-6 mol/（cm·min） and 2.18×10-6 mol/（cm·min） respectively.The higher the optical basicity of the slag was， the higher the hydrogen permeability was. CaO in the slag had a high affinity for water and can easily increase the hydrogen content in the steel ingot during electric arc remelting， while the addition of MgO in the slag system can significantly reduce hydrogen permeability. Under the research conditions， the influence of the slag system's constituents on its hydrogen permeability was more significant than the optical basicity.

Abstract Background Cystic Fibrosis causing mutations in the gene CFTR, reduce the activity of the CFTR channel protein, and leads to mucus aggregation, airway obstruction and poor lung function. A role for CFTR in the pathogenesis of other muco-obstructive airway diseases such as Chronic Obstructive Pulmonary Disease (COPD) has been well established. The CFTR modulatory compound, Ivacaftor (VX-770), potentiates channel activity of CFTR and certain CF-causing mutations and has been shown to ameliorate mucus obstruction and improve lung function in people harbouring these CF-causing mutations. A pilot trial of Ivacaftor supported its potential efficacy for the treatment of mucus obstruction in COPD. These findings prompted the search for CFTR potentiators that are more effective in ameliorating cigarette-smoke (CS) induced mucostasis. Methods Small molecule potentiators, previously identified in CFTR binding studies, were tested for activity in augmenting CFTR channel activity using patch clamp electrophysiology in HEK-293 cells, a fluorescence-based assay of membrane potential in Calu-3 cells and in Ussing chamber studies of primary bronchial epithelial cultures. Addition of cigarette smoke extract (CSE) to the solutions bathing the apical surface of Calu-3 cells and primary bronchial airway cultures was used to model COPD. Confocal studies of the velocity of fluorescent microsphere movement on the apical surface of CSE exposed airway epithelial cultures, were used to assess the effect of potentiators on CFTR-mediated mucociliary movement. Results We showed that SK-POT1, like VX-770, was effective in augmenting the cyclic AMP-dependent channel activity of CFTR. SK-POT-1 enhanced CFTR channel activity in airway epithelial cells previously exposed to CSE and ameliorated mucostasis on the surface of primary airway cultures. Conclusion Together, this evidence supports the further development of SK-POT1 as an intervention in the treatment of COPD.

Abstract Western criminological literature continues to focus on the binary control system (formal vs. informal), while China has long employed the trinary system (formal, semiformal, and informal) in its construction of social control. China scholars have investigated the prevalence and importance of the trinary control system as a key to understanding social control. However, there is a lack of research on their predictors. The current study aims at investigating the correlates of the prevalence and importance of the observed trinary control system in rural China. Using the sample data of 2343 respondents collected from 164 villages in rural China, the study found that both individual and village factors were the predictors of the prevalence of the observed controls exerted by village committees, a measure of semiformal control, and police, a measure of formal control, in maintaining community social order. Findings included the reported prevalence of four control mechanisms as significant predictors of their importance in community order maintenance. The implications of these findings are discussed.

Abstract The harmonic compensation of SAPF (shunt active power filter) may trigger harmonic oscillation due to the interaction with grid impedance and non‐linear loads, which distorts the power quality. In previous research, the relevant mechanism has been studied. However, the SAPF generally employs complex harmonic detection algorithm, which significantly increases the computational demands. The SAPF in full compensation mode can effectively solve this problem. But the modification of harmonic detection link may change the oscillation mechanism and previous stabilization methods need to be re‐evaluated. Therefore, this paper establishes the small signal model of the SAPF system in full compensation mode and investigates its oscillation mechanism. It is revealed that harmonic compensation and passive resonance collectively induce the harmonic oscillation. Based on the model, the working mechanism and application performance of the main stabilization methods proposed in the past (harmonic virtual admittance and harmonic compensation canceling) are analyzed and compared. However, neither of them can achieve effective stabilization without sacrificing the performance of compensation. Consequently, the harmonic phase compensation method is proposed to achieve the stabilization of the SAPF system in full compensation mode without affecting normal compensation. Finally, simulations and experiments are performed to verify the mechanism analysis and proposed methods.

The effects of high-pressure homogenization (HPH) at different pressures (30, 40, 50 and 60 MPa) on the hydration properties and crystallinity of bamboo shoot dietary fiber (BSDF) were studied as well as the effect of HPH-treated BSDF on the gel strength, water-holding capacity (WHC), protein secondary structure, chemical interactions and microstructure of heat-induced gels from shrimp surimi. The results showed that the WHC, oil-holding capacity (OHC), and swelling capacity of BSDF were positively correlated with homogenization pressure (P < 0.05). However, too small size of fiber particles affected its combination with hydrophilic groups, which could reduce the water absorption and swelling properties. X-ray diffraction (XRD) and scanning electron microscopic (SEM) results demonstrated that the crystallinity of BSDF decreased after medication, and the fiber bundle structure was destroyed, becoming loose and porous. Compared with the surimi added with unmodified BSDF, the gel strength of shrimp surimi added with BSDF treated at 30, 40, 50, and 60 MPa were increased by 10.04%, 17.87%, 28.76%, 28.49%, and WHC increased by 3.48%, 5.74%, 13.04%, and 12.70%, respectively. Fourier transform infrared (FTIR) spectral analysis showed that the contents of α-helix and random coil in the modified BSDF-supplemented shrimp surimi gel decreased, while the contents of β-sheet and β-turn increased significantly, suggesting more stable secondary structure of proteins. The results of chemical interaction analysis showed that modified BSDF promoted the hydrophobic interactions and the formation of disulfide bonds in shrimp surimi gel, with a more pronounced effect being observed with BSDF treated at 50 and 60 MPa. SEM results showed that shrimp surimi gel added with BSDF treated at 60 MPa had a more uniform and denser three-dimensional network structure. These results showed that HPH treatment could improve the hydration properties, and reduce the crystallinity of BSDF, and HPH-treated BSDF could significantly enhance the gelation properties of heat-induced shrimp surimi, which will provide a theoretical basis for improving the application of BSDF in shrimp surimi gels.

This paper introduced a novel semiring structure involving nonnegative integers, where operations depended on the comparison of the magnitudes of decimal digit sums. Consequently, a corresponding matrix semiring can be established on this commutative semiring. We showed that the 3-satisfiability problem can be polynomial-time reduced to solving systems of quadratic polynomial equations over this semiring. We proposed a key exchange protocol based on this matrix semiring, with its security relying on the two-sided digital circulant matrix action problem over this semiring. This scheme provides a novel cryptographic primitive for post-quantum cryptography.

Abstract 5-Fluorouracil (5-FU) is the primary treatment option for advanced gastric cancer. However, the current challenge lies in the absence of validated biomarkers to accurately predict the efficacy and sensitivity of 5-FU in individual patients. It has been confirmed that 5-FU can regulate tumor progression by promoting gasdermin E (GSDME, encoded by DFNA5) cleavage to induce pyroptosis. Lysine demethylase ALKBH4 has been shown to be upregulated in a variety of tumors to promote tumor progression. However, its role in gastric cancer is not clear. In this study, we observed a significant upregulation of ALKBH4 expression in gastric cancer tissues compared to adjacent normal tissues, indicating its potential as a predictor for the poor prognosis of gastric cancer patients. On the contrary, GSDME exhibits low expression levels in gastric cancer and demonstrates a negative correlation with poor prognosis among patients diagnosed with gastric cancer. In addition, we also found that high expression of ALKBH4 can inhibit pyroptosis and promote the proliferation of gastric cancer cells. Mechanistically, ALKBH4 inhibits GSDME activation at the transcriptional level by inhibiting H3K4me3 histone modification in the GSDME promoter region, thereby reducing the sensitivity of gastric cancer cells to 5-FU treatment. These findings provide further insight into the regulatory mechanisms of ALKBH4 in the progression of gastric cancer and underscore its potential as a prognostic marker for predicting the sensitivity of gastric cancer cells to 5-FU treatment.

Abstract. Background:. Homoharringtonine (HHT) is an effective anti-inflammatory, anti-viral, and anti-tumor protein synthesis inhibitor that has been applied clinically. Here, we explored the therapeutic effects of HHT in a mouse heart transplant model. Methods:. Healthy C57BL/6 mice were used to observe the toxicity of HHT in the liver, kidney, and hematology. A mouse heart transplantation model was constructed, and the potential mechanism of HHT prolonging allograft survival was evaluated using Kaplan–Meier analysis, immunostaining, and bulk RNA sequencing analysis. The HHT-T cell crosstalk was modeled ex vivo to further verify the molecular mechanism of HHT-induced regulatory T cells (Tregs) differentiation. Results:. HHT inhibited the activation and proliferation of T cells and promoted their apoptosis ex vivo. Treatment of 0.5 mg/kg HHT for 10 days significantly prolonged the mean graft survival time of the allografts from 7 days to 48 days (P

Soil metabolomics is an emerging approach for profiling diverse small molecule metabolites, i.e., metabolomes, in the soil. Soil metabolites, including fatty acids, amino acids, lipids, organic acids, sugars, and volatile organic compounds, often contain essential nutrients such as nitrogen, phosphorus, and sulfur and are directly linked to soil biogeochemical cycles driven by soil microorganisms. This paper presents an overview of methods for analyzing soil metabolites and the state-of-the-art of soil metabolomics in relation to soil nutrient cycling. We describe important applications of metabolomics in studying soil carbon cycling and sequestration, and the response of soil organic pools to changing environmental conditions. This includes using metabolomics to provide new insights into the close relationships between soil microbiome and metabolome, as well as responses of soil metabolome to plant and environmental stresses such as soil contamination. We also highlight the advantage of using soil metabolomics to study the biogeochemical cycles of elements and suggest that future research needs to better understand factors driving soil function and health.

Abstract The triggering receptor expressed on myeloid cells 2 (TREM2) is an immune receptor that affects cellular phenotypes by modulating phagocytosis and metabolism, promoting cell survival, and counteracting inflammation. Its role in renal injury, in particular, unilateral ureteral obstruction (UUO) or ischemia-reperfusion injury (IRI)-induced renal injury remains unclear. In our study, WT and Trem2 −/− mice were employed to evaluate the role of TREM2 in renal macrophage infiltration and tissue injury after UUO. Bone marrow-derived macrophages (BMDM) from both mouse genotypes were cultured and polarized for in vitro experiments. Next, the effects of TREM2 on renal injury and macrophage polarization in IRI mice were also explored. We found that TREM2 expression was upregulated in the obstructed kidneys. TREM2 deficiency exacerbated renal inflammation and fibrosis 3 and 7 days after UUO, in association with reduced macrophage infiltration. Trem2 −/− BMDM exhibited increased apoptosis and poorer survival compared with WT BMDM. Meanwhile, TREM2 deficiency augmented M1 and M2 polarization after UUO. Consistent with the in vivo observations, TREM2 deficiency led to increased polarization of BMDM towards the M1 proinflammatory phenotype. Mechanistically, TREM2 deficiency promoted M1 and M2 polarization via the JAK-STAT pathway in the presence of TGF-β1, thereby affecting cell survival by regulating mTOR signaling. Furthermore, cyclocreatine supplementation alleviated cell death caused by TREM2 deficiency. Additionally, we found that TREM2 deficiency promoted renal injury, fibrosis, and macrophage polarization in IRI mice. The current data suggest that TREM2 deficiency aggravates renal injury by promoting macrophage apoptosis and polarization via the JAK-STAT pathway. These findings have implications for the role of TREM2 in the regulation of renal injury that justify further evaluation.

ObjectiveThis study integrates network pharmacology and metabolomics techniques to explore the potential regulatory mechanisms of Res on gouty arthritis (GA).MethodsNetwork pharmacology was used to predict the mechanism of Res in regulating GA, and methods such as HE staining, ELISA, immunohistochemistry, Real-time PCR, Western blot, and molecular docking were used to verify the role of NF-κB, MAPK, and JAK/STAT inflammatory signaling pathways in the MSU-induced GA rat model. In addition, non-targeted metabolomics techniques were combined to further investigate the mechanism of Res in treating GA.ResultsThe results of network pharmacology showed that Res may exert its therapeutic effects through the NF-κB signaling pathway. Animal experiments demonstrated that in the MSU-induced GA rat model, pathological damage, serum biochemical indicators, and levels of inflammatory factors were significantly increased, and the NF-κB signaling pathway was activated. The intervention of Res significantly reduced pathological damage, serum biochemical indicators, levels of inflammatory factors, and the activation of NF-κB, MAPK, and JAK/STAT signaling pathways in the model rats. Metabolomics results showed that Res could improve the metabolic trajectory deviations in serum and joint fluid of GA model rats. Through related metabolic pathway analysis, the most affected metabolic pathways were found to be Sphingolipid metabolism, Glycerophospholipid metabolism, Phenylalanine, tyrosine and tryptophan biosynthesis, Pantothenate and CoA, Citrate cycle (TCA cycle), and Arachidonic acid metabolism.ConclusionResveratrol can regulate the biosynthetic pathways of arachidonic acid, phenylalanine, tyrosine, and tryptophan, pantothenic acid and CoA biosynthesis pathways, TCA cycle, and other metabolic pathways, thereby regulating the NF-κB, MAPK, and JAK/STAT3 signaling pathways, and inhibiting the acute inflammatory response during GA attacks, showing characteristics of multi-pathway and multi-target action.

BackgroundKidney transplantation (KT) is the best treatment for patients with end-stage renal disease. However, postoperative complications remain the main issues faced during KT recovery period. Posttransplant lymphoproliferative disorders (PTLD) are one of the severe and life-threatening complications that occur after KT while the recipient is undergoing immunosuppressive therapy. PTLD risk factors include Epstein-Barr virus (EBV) infection, the cumulative degree of immunosuppression, as well as genetic aspects. PTLD is more common in the transplanted organ itself and its surroundings, and the central nervous system, while PTLD involving the pharyngeal soft tissue is relatively rare, with only a few reported case reports. Therefore, systematic experience is scarce regarding whether the treatment or the care.Case presentationHerein, we report a 41-year-old male, underwent a reproductive KT due to chronic renal insufficiency. Recurrent fever, pharyngeal pain, and bilateral cervical lymph node enlargement were recurred during five years’ follow-up after KT surgery. In this inpatient experience, the patient vomited a large amount of blood from the oropharynx, then the tonsil artery was ligated by emergency operation. EBV-associated T/NK cell monomorphic PTLD was eventually diagnosed by blood EBV DNA test, pharyngeal biopsy, and corresponding pathological examination. After six cycles of R-CHOP chemotherapy, the clinical symptoms and laboratory tests changed into normal. Subsequent three years’ follow-up shows no tumor recurrence and good transplant kidney function.ConclusionThis rare case report describes a manifestation of PTLD with pharyngeal involvement. Early diagnosis using histopathological examination is crucial to prevent damage to the throat and airway, and even life-threatening conditions. Discontinuing immunosuppression and starting systemic treatment can help in disease regression. Since the low incidence of this disease, limited clinical experience, and limited data, our experience with a smooth recovery through efficacy treatment and nursing can provide a reference for the development of new clinical drugs and diagnostic and treatment plans of patients with PTLD in the future.

Introduction: Acute promyelocytic leukemia (APL) is a subtype of acute myeloid leukemia (AML), with myeloid sarcoma (MS) seldom occurring at onset. Myelofibrosis (MF) is a condition characterized by megakaryocytic proliferation and atypia with an array of clinical findings, but MF secondary to APL treatment is extremely rare. MF secondary occurring after treatment of APL with MS as the initial presentation has not been reported. Case report: A 73-year-old male was admitted to our hospital in August 2016, presenting with a pain in his left shoulder, followed by right shoulder and bilateral hip pain. A progressive increase in a mass in the right sternoclavicular joint was also observed. After comprehensive examines, he was diagnosed as APL with MS as the initial manifestation in April 2017. The patient then underwent induction, consolidation, and maintenance therapies until August 2019 and achieved complete remission. However, twice examinations in December 2020 and September 2022 confirmed that MF was present. Conclusion: This is the first case of MF secondary occurring after treatment of APL with MS. Early recognition of MS in APL patients is crucial for timely intervention and treatment initiation. Meticulous diagnostic work-up and careful monitoring during treatment are necessary to detect potential complications, which may significantly impact the patients' outcome. Also, a comprehensive management approach encompassing induction, consolidation, and maintenance therapies should be adopted to ensure optimal therapeutic responses and reduce the risk of recurrence or treatment-related complications.

IntroductionGradual pollen presentation is a reproductive strategy for plants to improve the efficiency and accuracy of pollen export and acceptance as well as to promote outcrossing. Vaccinium corymbosum Berkeley has a typical mechanism of gradual pollen presentation.MethodsIn this study, we used indoor and outdoor observations to investigate the floral characteristics, pollination characteristics and breeding system of this species in open ground and greenhouse.ResultsThe results showed that the single white, inverted bell-shaped flower of V. corymbosum was able to effectively avoid the negative effects of rainfall, nectar dilution and pollen removal by wind, and improved pollen viability and the duration of stigma receptivity. The setting rate of ‘Northland’ is higher than open ground by artificial pollination in greenhouse, therefore, greenhouses are more suitable for ‘Northland’ reproduction. The gradual pollen presentation prolongs dispersal time, promotes pollinator participation in the pollination process, increases staminate fitness, and provides for nectar feeding by pollinators as well as pollen export. Bombus spp. and A. m. ligustica were effective pollinators in the open ground and greenhouse, and the pollination efficiency of Bombus spp. was higher than that of A. m. ligustica, and greenhouse pollination is more efficient than open ground. The coincidence of the peak period of pollinator visits with the period of highest pollen viability and optimal pollination of stigmas, as well as the high nectar production, could maximize the frequency of pollinator visits and increase the chances of successful pollination as well as the pollination efficiency.DiscussionTherefore, it is important to study the floral characteristics of V. corymbosum Berkeley and the mechanism of gradual pollen presentation in order to improve the pollination efficiency and promote the success of outcrossing, which can provide a theoretical basis for the breeding of V. corymbosum.

Background and AimsPortal vein tumor thrombus (PVTT) occurs frequently in hepatocellular carcinoma (HCC) patients. However, there is currently no satisfactory treatment. Radiotherapy (RT) and tyrosine kinase inhibitors (TKI) are currently commonly used. However, whether their combined use provides a survival benefit is debatable. This retrospective study compared the efficacy and safety between radiotherapy plus lenvatinib (RT + L) and radiotherapy plus sorafenib (RT + S) in the treatment of hepatocellular carcinoma with portal vein tumor thrombus (PVTT).MethodsAmong patients with PVTT who received RT + L or RT + S between March 2017 and September 2022, the primary endpoints were overall survival (OS) and progression-free survival (PFS). The secondary endpoints were objective response rate (ORR), disease control rate (DCR), and incidence of treatment-related adverse effects. The prognostic factors were also assessed.ResultsThe analysis included 152 patients (RT + L: 48; RL: 25; RT + S: 55; S: 24). Compared with the RT + S group, the RT + L group had a longer OS and PFS. Among patients with type I/II PVTT, the median OS times were 19.8 months and 13.5 months (p = 0.047) and the median PFS was 12.3 months and 7.3 months (p = 0.042), respectively. And the median OS of the patients with type I/II PVTT were 14.4 months and 8.3 months (p = 0.030) and the median PFS was 8.3 months and 6.2 months (p = 0.026). ORR and DCR in RT + L group (25.0% and 75.0%) were also little higher than those in RT + S group (20.0% and 70.9%), but not statistically significant. In univariate analysis, etiology, Type of PVTT, alpha-fetoprotein (AFP) level, Child–Pugh score, and treatment method influenced OS. Multivariate analysis confirmed that treatment method, etiology, alpha-fetoprotein (AFP) level, and Child–Pugh score were independent prognostic factors for OS. Similar safety profiles were observed in the RT + L and RT + S groups. The most common adverse events were myelosuppression, decreased liver function, fatigue, diarrhea, nausea, and vomiting. Most adverse reactions were grade 1–2.ConclusionThe side effects of radiotherapy plus lenvatinib were acceptable. Compared to RT + S, RT + L had good efficacy in the treatment of hepatocellular carcinoma with PVTT. Validation is needed in prospective studies with larger sample sizes.

According to official statistics, cancer remains the main reason of death and over 50% of patients with cancer receive radiotherapy. However, adverse consequences after radiation exposure like radiation-induced skin reactions (RISR) have negative or even fatal impact on patients’ quality of life (QoL). In this review we summarize the mechanisms and managements of RISRs, a process that involve a variety of extracellular and intracellular signals, among which oxidative stress (OS) are now commonly believed to be the initial part of the occurrence of all types of RISRs. As for the management of RISRs, traditional treatments have been widely used but without satisfying outcomes while some promising therapeutic strategies related to OS still need further researches. In the context we discuss how OS leads to the happening of RISRs of different types, hoping it can shed some light on the exploration of new countermeasures.

PurposeMedication overuse headache (MOH) is a chronic headache caused by regular overuse of medications. OnabotulinumtoxinA (BoNTA) is used for preventive treatment of MOH. However, its efficacy and safety remain controversial.MethodsSeven online databases (Cochrane Library, Embase, Medline, PubMed, China National Knowledge Infrastructure, Wanfang data, and Chinese BioMedical Literature Database) were searched for relevant articles published between January 2002 and March 2024. We included randomized controlled trials (RCTs) and cohort studies on the treatment of MOH using BoNTA versus a placebo or other active treatments.ResultsWe retrieved 487 articles in the database search. Of these, four eligible RCTs were identified after detailed screening. A total of 1,259 patients with MOH (622 patients treated with BoNTA, 607 with placebo, and 30 with topiramate) were included in the four RCTs. We found that BoNTA significantly reduced headache frequency compared with placebo (mean difference, 1.89; 95% confidence interval (CI), 1.11–2.67; I2 = 0%; p

Background: The pacing strategy embodies the tactical behavior of athletes in distributing their energy across different segments of a race; therefore, a quantitative analysis of pacing strategies in marathon races could deepen the understanding of both pacing behavior and physical capacity in marathon athletics. Objective: The objective of this systematic review was to synthesize and characterize pacing strategies in marathon road races by exploring the categories of pacing strategies and the factors that influence these strategies during marathon events. Methods: Preferred Reporting Items for Systematic Reviews guidelines were followed for systematic searches, appraisals, and syntheses of literature on this topic. Electronic databases such as Science Direct, SPORTDiscuss, PubMed, and Web of Science were searched up to July 2024. Records were eligible if they included pace performance measurements during competition, without experimental intervention that may influence their pace, in healthy, adult athletes at any level. Results: A total of 39 studies were included in the review. Twenty-nine were observational studies, and 10 were experimental (randomized controlled trials). The assessment of article quality revealed an overall median NOS score of 8 (range 5–9). The included studies examined the pacing profiles of master athletes and finishers in half-marathon (n = 7, plus numbers compared to full marathon), full-marathon (n = 21), and ultramarathon (n = 11) road races. Considering that some studies refer to multiple pacing strategies, in general, 5 studies (∼13 %) reported even pacing, 3 (∼8 %) reported parabolic pacing, 7 (∼18 %) reported negative pacing, and 30 (∼77 %) reported positive pacing during marathon competitions. Gender, age, performance, pack, and physiological and psychological factors influence pacing strategies. Conclusion: This study synthesized pacing performance in marathons and highlighted the significance of examining pacing strategies in these events, offering valuable insights for coaches and athletes. Several key findings were highlighted: (1) pacing profiles and pacing ranges were identified as the primary indicators of pacing strategies; (2) the pacing strategy was found to be dynamic, with the most substantial effects attributed to gender and distance; and (3) three distinct types of pacing strategies for marathons were classified: positive, negative, and even pacing. These findings advance the understanding of marathon pacing strategies by shedding light on the factors that influence athletes’ pacing decisions and behaviors. Additionally, these findings offer practical benefits, aiding athletes in making well-informed tactical choices and developing effective pace plans to enhance marathon performance. However, due to the complex nature of marathon racing, further research is required to explore additional factors that might impact pacing strategies. A better grasp of optimal pacing strategies will foster progress in this area and serve as a basis for future research and advancements.

Mesenchymal stem cells (MSCs) are naturally-derived regenerative materials that exhibit significant potential in regenerative medicine. Previous studies have demonstrated that MSCs-based therapy can improve heart function in ischemia-injured hearts, offering an exciting therapeutic intervention for myocardial ischemic infarction, a leading cause of worldwide mortality and disability. However, the efficacy of MSCs-based therapies is significantly disturbed by the myocardial microenvironment, which undergoes substantial changes following ischemic injury. After the ischemic injury, blood vessels become obstructed and damaged, and cardiomyocytes experience ischemic conditions. This activates the hypoxia-induced factor 1 (HIF-1) pathway, leading to the rapid production of several cytokines and chemokines, including vascular endothelial growth factor (VEGF) and stromal-derived factor 1 (SDF-1), which are crucial for angiogenesis, cell migration, and tissue repair, but it is not sustainable. MSCs respond to these cytokines and chemokines by homing to the injured site and participating in myocardial regeneration. However, the deteriorated microenvironment in the injured myocardium poses challenges for cell survival, interacting with MSCs, and constraining their homing, retention, and migration capabilities, thereby limiting their regenerative potential. This review discusses how the deteriorated microenvironment negatively affects the ability of MSCs to promote myocardial regeneration. Recent studies have shown that optimizing the microenvironment through the promotion of angiogenesis can significantly enhance the efficacy of MSCs in treating myocardial infarction. This approach harnesses the full therapeutic potential of MSCs-based therapies for ischemic heart disease.

Karst areas are often characterized by fragile ecological systems, and environmental pollution has increased the pressures on people living in such regions. This study aimed to investigate the status of pollution caused by heavy metals (Pb, Cd, Hg, As, and Cu) in soils based on different land uses in Changshun County, a karst area in southwestern China. Soil samples were collected from natural forestlands (NFLs), natural brush lands (NBLs), natural pasture lands (NPLs), artificial forestlands (AFLs), artificial brush lands (ABLs), and artificial pasture lands (APLs) for evaluation. The results suggest that the soil profile characteristics of the heavy metals studied herein vary significantly among different land uses. The heavy metal concentrations in all soil samples collected from NFLs were lower than those in samples from other land uses. Forest trees can protect soil from heavy metal pollution caused by atmospheric deposition; this is especially true for Hg. In cultivated forestlands and brush lands, special attention should be devoted to Cd pollution in the soil, which may be caused by the use of fertilizers. Changing both natural and artificial pastoral lands to forestlands could benefit the local ecosystems as it may reduce Hg contamination.

BackgroundThis study aimed to explore the correlation between psychological capital and music performance anxiety among Chinese college students majoring in music. Additionally, the study evaluated the chain mediating effects of self-esteem and flow experience.MethodsThree hundred twenty-nine college students pursuing a music major from institutions in Eastern China participated in the study. They were asked to complete the Psychological Capital, Self-Esteem, Flow Experience, and Music Performance Anxiety Scales. Descriptive statistics and correlation analysis were performed, and sophisticated mediation models were created.ResultsThe results suggest that self-esteem and flow experience play a role in connecting psychological capital and music performance anxiety. Moreover, the ultimate model elucidates significant connections linking psychological capital to self-esteem and flow experience in music performance anxiety.ConclusionThis study offers useful insights for future interventions aimed at mitigating music performance anxiety through the cultivation of students’ psychological capital, self-esteem, and flow experiences.

BackgroundEvidence shows that the total flavonoids of Rhizoma Drynariae (TFRD) can improve bone mineral density (BMD). However, there is no evidence to summarize the improvement of biochemical indicators of bone metabolism (BIBM).MethodsThe PubMed, Web of Science, Cochrane Library, Embase, Chinese National Knowledge Infrastructure (CNKI), Wanfang Database, Chongqing VIP Information Database (VIP) and SinoMed were searched from inception to 6 May 2024. The final included studies performed meta-analyses using RevMan 5.3.ResultsNine randomized controlled trials (RCTs) were ultimately included. The TFRD group had higher bone gla protein (BGP) and type I procollagen-N-propeptide (PINP) compared to the Other therapies (WMD: 5.11; 95% CI: 3.37, 6.84; p < 0.00001; WMD: 13.89; 95% CI: 11.81, 15.97; p < 0.00001). The tartrate-resistant acid phosphatase (TRACP) decreased significantly (WMD: −1.34; 95% CI: −1.62, −1.06; p < 0.00001). The alkaline phosphatase (ALP) increased significantly (WMD: 7.47; 95% CI: 6.29, 8.66; p < 0.00001). There were no significant differences in serum calcium (SC) or serum phosphorus (SP) levels between the TFRD and control groups (WMD: 0.08; 95% CI: −0.04, 0.20; p = 0.17; WMD: 0.02; 95% CI: −0.02, 0.05; p = 0.36).ConclusionTFRD can stimulate bone formation and prevent bone resorption in osteoporosis (OP) patients, but it has no effect on SC and SP.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/.

Background Endovascular treatment (EVT) has been demonstrated to be effective for patients with tandem occlusion. The efficacy and safety of intravenous thrombolysis before EVT in patients with tandem occlusion remain debatable. Methods and Results We conducted a systematic review and meta‐analysis with PubMed, EMBASE, and the Cochrane Library from inception to September 2023. The primary outcome was functional independence, defined as a modified Rankin Scale score of 0 to 2 at 90 days. The secondary outcomes included the successful recanalization rate, symptomatic intracerebral hemorrhage, and mortality at 90 days. In total, 9 studies with 1838 enrolled participants were identified. Our results showed that, compared with treatment with EVT alone, intravenous thrombolysis before EVT was associated with a greater proportion of functional independence at 90 days (odds ratio [OR], 1.39 [95% CI, 1.14–1.69]; P=0.001), a greater rate of successful recanalization (OR, 1.45 [95% CI, 1.11–1.89]; P=0.007) and decreased mortality (OR, 0.68 [95% CI, 0.50–0.93]; P=0.02). Furthermore, there was no significant difference in symptomatic intracerebral hemorrhage between the intravenous thrombolysis plus EVT group and the EVT alone group (OR, 1.16 [95% CI, 0.79–1.70]; P=0.45). Conclusions In patients with acute ischemic stroke and tandem occlusion, intravenous thrombolysis before EVT was associated with a greater rate of favorable functional outcomes and successful recanalization and a lower mortality rate without an increased risk of symptomatic intracerebral hemorrhage compared with patients receiving EVT alone.

The global spread and distribution of antibiotic resistance genes (ARGs) has received much attention whereas knowledge about the transmission of ARGs from one matrix to another is still insufficient. In this study, the paddy fields fertilized with chemical fertilizer, swine compost, and no fertilizer were investigated to assess the transfer of ARGs from soil to rice. Soil and plant samples were collected at day 0, 7, 30 and 79 representing various stages of paddy growth. High throughput qPCR was applied to quantify ARGs using a set of 144 primers. Gene copy number of ARGs measured in soil initially decreased and then increased in soil with no fertilizer and chemical fertilizer, indicating that crop planting and flooding conditions did influence the ARGs profiles in soil. Application of swine compost significantly enhanced the relative abundance and gene copy number of ARGs in paddy soil. Rice seedlings contained substantial amount of ARGs and their relative abundance continually decreased after transplant. Compared with initial stage, detection frequencies of ARGs increased in soil without swine compost at harvest time (day 79), indicating the transmission of ARGs from irrigation water to soil. Detection frequencies of ARGs increased in soil and rice root with swine compost at harvest time, indicating the transfer of ARGs from swine compost to soil and rice root. There was no significant difference in abundance and diversity of ARGs in rice grains with these three different fertilizations. The source of the ARGs in rice grain still needs further exploration.