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Abstract Objective Red blood cell distribution width (RDW) is a marker of inflammation and oxidative stress, and its elevation has been associated with poor outcomes in critically ill patients. This meta-analysis aimed to evaluate the association between RDW at admission and short-term mortality in patients with severe burn injury. Methods We systematically searched PubMed, Embase, and Web of Science up to August 2024, following PRISMA 2020 and Cochrane guidelines. Cohort studies of adult severe burn patients who reported RDW at admission and short-term mortality were included. Meta-analyses were performed using random-effects models, with mean difference (MD) and relative risk (RR) calculated for RDW and mortality outcomes, respectively. Subgroup and sensitivity analyses were conducted to assess heterogeneity. Results Nine cohort studies involving 5268 patients were included. Overall, RDW at admission was higher in nonsurvivors compared to survivors [MD: 0.95%, 95% confidence interval (CI): 0.52–1.38, p

Abstract Objective Femoral neck fractures (FNFs) are a common orthopedic type, and there are many treatment methods for it, and cannulated screw internal fixation is currently one of the main treatment methods. The choice of fully threaded cannulated screw (FCS) or partially threaded cannulated screw (PCS) remains controversial. Therefore, we performed this meta-analysis to evaluate the outcomes of FCS and PCS in the treatment of FNF. Methods Articles published before 29 April, 2024 were selected from PubMed, Embase, the Cochrane Library, and CNKI, using the PRISMA guidelines. Two independent reviewers searched and assessed the literature. The PICOS criteria were used to ensure that the included studies met the inclusion criteria. We used RevMan 5.3. Software to perform analysis. Results Compared with the PCS group, the FCS group had a lower femoral head necrosis rate (OR 0.60, 95% CI 0.37–0.98, P = 0.04), lower internal fixation failure rate (OR 0.37, 95% CI 0.22–0.62, P = 0.0002) and lower femoral neck shortening rate (OR 0.27, 95% CI 0.19–0.40, P

As a lightweight structural material, Mg alloy is expected to have a broad application prospect. However, due to its poor wear resistance and corrosion resistance, its application range is greatly limited. In this paper, wear resistant and corrosion resistant coatings for WC-12Co were prepared on AZ31B substrate by HVOF thermal spraying. Considering the random probability distribution of particle size and the random distribution of substrate surface roughness under different sandblasting processes, the stochastic multi-particle deposition model was established based on the Coupling Euler Lagrange (CEL) method. The influence of substrate surface roughness on temperature field, stress field and strain field during coating formation was revealed. And the coating pores formation mechanism was revealed with experiments. The results show that the local stress at the bonding surface on the substrate is the largest. Centered on the bonding surface, the substrate internal stress decreases with the increase of the distance from the bonding surface. With the substrate surface roughness increasing, the coating internal residual stress slightly decreases. The local density changes caused by the particle spatial distribution randomness can produce stacking defects and then form pores. The substrate surface roughness has a small effect on the coating internal porosity.

The aim of this study is to address the limitations of convolutional networks in recognizing modulation patterns. These networks are unable to utilize temporal information effectively for feature extraction and modulation pattern recognition, resulting in inefficient modulation pattern recognition. To address this issue, a signal modulation recognition method based on a two-way interactive temporal attention network algorithm has been developed. A two-way interactive temporal network is designed on the basis of the long and short-term memory network with the objective of enhancing the contextual connection of the temporal network. The output of the temporal network is attentively weighted using the soft attention mechanism. The proposed algorithm exhibited enhanced overall, average, and maximum recognition rates at varying signal-to-noise ratios, with an increase of 10.34%, 8.33%, and 3.33%, respectively, in comparison to other algorithms within the Radio Machine Learning (RML) 2016.10b dataset. Furthermore, the modulated signal recognition accuracy was as high as 92.84%, with an average increase in the Kappa coefficient of 12.28%. The Kappa coefficient in the Communication Signal Processing Benchmark for Machine Learning (CSPB.ML2018) 2018 dataset was 0.62, representing an average increase of 10.32% over other algorithms. The results demonstrate that the proposed recognition method can enhance the network's accuracy in recognizing modulated signals. Moreover, it has potential applications in modulation pattern recognition in automatic systems for wireless communications.

Dysbiosis of the intestinal microbiota is prevalent among patients with colorectal cancer (CRC). This study aims to explore the anticancer roles of the fecal microbiota in inhibiting the progression of colorectal cancer and possible mechanisms. The intestinal microbial dysbiosis in CRC mice was significantly ameliorated by fecal microbiota transplantation (FMT), as indicated by the restored ACE index and Shannon index. The diameter and number of cancerous foci were significantly decreased in CRC mice treated with FMT, along with the restoration of the intestinal mucosal structure and the lessening of the gland arrangement disorder. Key factors in oxidative stress (TXN1, TXNRD1, and HIF-1α); cell cycle regulators (IGF-1, BIRC5, CDK8, HDAC2, EGFR, and CTSL); and a critical transcription factor of the innate immune signal pathway (IRF5) were among the repressed oncogenic targets engaged in the FMT treatment of CRC. Correlation analysis revealed that their expressions were positively correlated with uncultured_bacterium_o_Mollicutes_RF39, Rikenellaceae_RC9_gut_group, and negatively correlated with Bacillus, Marvinbryantia, Roseburia, Angelakisella, Enterorhabdus, Bacteroides, Muribaculum, and genera of uncultured_bacterium_f_Eggerthellaceae, uncultured_bacterium_f_Xanthobacteraceae, Prevotellaceae_UCG-001, uncultured_bacterium_f_Erysipelotrichaceae, uncul-tured_bacterium_f_Lachnospiraceae, uncultured_bacterium_f_Ruminococcaceae, Eubacterium_coprostanoligenes_group, Ruminococcaceae_UCG-005, and uncultured_bacterium_f_Peptococcaceae. This study provides more evidence for the application of FMT in the clinical treatment of CRC.

Gully erosion is one of the most severe types of land degradation, hindering food production and sustainable agricultural development. However, the historical evolution process and the impact of land use change on gully erosion remain unclear. To address this issue, we conducted a field investigation on gully erosion in 2018 and interpreted land use and gullies using historical remote sensing images in 1968 and 1978 over an area of 84.48 km2. The study found that from 1968 to 1978 to 2018, all gully morphological parameters including gully length density and gully areal density increased significantly. The main origin of gully erosion found was from dry farmland. The annual soil loss rate induced by gully erosion was 1.46 mm during 1968–2018. Gully erosion rates were higher during 1968–1978 than during 1978–2018. Furthermore, the length, areal and volumetric erosion rates in gullies formed by multiple gullies merging was greater than that of newly formed gullies (NFG) and gullies developing continuously from a single pre-existing gully, while the widening rate of NFG was highest. The susceptibility of land use types to gully erosion was in the order of woodland

Abstract Background To establish a strategy for stem cell-related tissue regeneration therapy, human gingival mesenchymal stem cells (hGMSCs) were loaded with three-dimensional (3D) bioengineered Matrigel matrix scaffolds in high-cell density microtissues to promote local tissue restoration. Methods The biological performance and stemness of hGMSCs under 3D culture conditions were investigated by viability and multidirectional differentiation analyses. A Sprague‒Dawley (SD) rat full-thickness buccal mucosa wound model was established, and hGMSCs/Matrigel were injected into the submucosa of the wound. Autologous stem cell proliferation and wound repair in local tissue were assessed by histomorphometry and immunohistochemical staining. Results Three-dimensional suspension culture can provide a more natural environment for extensions and contacts between hGMSCs, and the viability and adipogenic differentiation capacity of hGMSCs were significantly enhanced. An animal study showed that hGMSCs/Matrigel significantly accelerated soft tissue repair by promoting autologous stem cell proliferation and enhancing the generation of collagen fibers in local tissue. Conclusion Three-dimensional cell culture with hydrogel scaffolds, such as Matrigel, can effectively improve the biological function and maintain the stemness of stem cells. The therapeutic efficacy of hGMSCs/Matrigel was confirmed, as these cells could effectively stimulate soft tissue repair to promote the healing process by activating the host microenvironment and autologous stem cells. Graphical abstract

Light‐emitting diodes (LEDs) are widely used in lighting and display applications. Carbon quantum dots (CQDs), which have high biocompatibility, high resistance to photobleaching, and full‐spectrum luminescence, have inherent advantages as fluorescent materials for LED devices. Herein, multicolor CQDs are prepared by a new reagent engineering strategy due to the difference of effective conjugate length and the surface electron‐withdrawing groups of CQDs. White CQDs are realized by mixing blue, green, and red CQDs proportionally. Then, the aggregation‐caused quenching phenomenon of CQDs is suppressed through the hydrogen‐bonding network of cellulose nanofibrils (CNFs). Multicolor fluorescent films are prepared from CQDs and CNFs by simple mixing and casting methods. Finally, thin‐film encapsulation based on the photosensitive resin ABPE‐10 coating can be realized and rapidly assembles into fluorescent films with different light‐emitting colors into LED devices, leading to have superior thermal performance compared with conventional LEDs. White LEDs have excellent white‐light illumination performance, with Commission Internationale de L’Eclairage color coordinates of (0.33, 0.37), a correlated color temperature of 5688 K, and a color rendering index of 86. This strategy provides a convenient and scalable pathway for low‐cost, environmentally friendly, and high‐performance CQDs‐based LEDs.

Biosensing is vital for many areas like disease diagnosis, infectious disease prevention, and point-of-care monitoring. Microfluidics has been evidenced to be a powerful tool for biosensing via integrating biological detection processes into a palm-size chip. Based on the chip structure, microfluidics has two subdivision types: open microfluidics and closed microfluidics, whose operation methods would be diverse. In this review, we summarize fundamentals, liquid control methods, and applications of open and closed microfluidics separately, point out the bottlenecks, and propose potential directions of microfluidics-based biosensing.

Objective To establish a method for the simultaneous quantitative analysis of etomidate and its metabolite etomidate acid in blood, and to discuss its application value in actual cases. Methods Acetonitrile precipitate protein method was used, and C18 column was selected. Gradient elution was performed with acetonitrile and 5 mmol/L ammonium acetate within 6 min. Electrospray ionization source in positive ion mode was used. The internal standard etomidate acid-d5 was obtained by etomidate-d5 alkaline hydrolysis reaction. Ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） was used for quantitative analysis. The methodological verification was conducted. Results Etomidate and etomidate acid in blood showed good linear relationship in the quantitative linear range （r>0.999）, with the lower limit of quantification was 2.5 ng/mL and 7.5 ng/mL, respectively. The accuracy, precision, recovery rate, and matrix effect of the method met the professional verification standards. The practical application results showed that etomidate and etomidate acid could be detected in the blood of the abusers, and their mass concentrations ranged from 17.24 to 379.93 ng/mL. Conclusion The method established in this study can simultaneously quantify etomidate and etomidate acid in blood, which is simple and convenient to operate with accuracy. It can meet the detection needs of actual cases and provide technical support for law enforcement to crack down on etomidate abuse.

The complex field environment under conservation tillage aggravates the vibration during a planter’s operation, affecting the sowing quality and fertilization depth. Studying its vibration characteristics can help to realize active vibration reduction control of planter row units. To this end, this paper took a four-row no-till planter as the research object. By establishing a field vibration model of the planter row unit, the factors affecting the vibration of the unit were clarified, and stubble height, working speed and the additional weight of the planter were used as experimental factors in carrying out field orthogonal experiments. In our experiment, we collected and analyzed vibration data on the four-row planter row units and the frame at different positions to explore the influence of various factors on the vibration characteristics of the planter. The experimental results showed that the working speed was the most important factor affecting the vibration of the planter, and the impact of stubble height and additional weight on the amplitude of the planter was more significant at low speed (1.5 m/s) than that at high speed (2.5 m/s). The difference in amplitude of each planter unit in the lateral direction was the largest, the average amplitude range of which was 1.898 m/s2. The vibration energy of each planter row unit under different working conditions was mainly concentrated in the range of 10–50 Hz. However, the three-point hitch of the planter transmitted the vibration excitation of the tractor, causing 110–120 Hz high-frequency vibration of the inner row units, while the outer row units were less affected, with the vibration energy, in the range above 100 Hz, being 2.5 dB smaller than that on the inner side. The right ground wheel transmission device was abnormal, which worked together with the excitation transmitted by the three-point hitch, making the average vibration acceleration amplitude of the planter row units on the right side in the lateral direction more than 0.522 m/s2 higher than that of the units on the left side. Therefore, different vibration reduction forces need to be applied according to the position of the planter row unit, so that the units can avoid the natural frequency of the frame (115 Hz) when vibrating. This study can provide a reference for active vibration reduction control and improvements in sowing quality for high-speed no-till planters.

In response to the problems of low control accuracy, single detection of operating parameters, and insufficient collaborative control of unmanned fertilization in field fertilization operations, this paper proposes an adaptive control strategy for fertilizer discharging shaft speed based on segmented linear interpolation method. By constructing a relationship model between fertilizer discharging shaft speed and motor control signals in different speed ranges, the on-site self-calibration of fertilizer discharging shaft speed and the precise control of the fertilization rate is achieved. At the same time, real-time detection and warning technology for multiple working condition parameters were integrated, and a bus communication protocol between unmanned tractors and variable-rate fertilizer applicators was developed. A variable-ratefertilization monitoring system based on unmanned tractors was developed, and actual experimental tests were conducted to test the system’s performance. Among them, the calibration test results of fertilization rate showed that the discharging rate of the fertilizer apparatuses (p) was negatively correlated with the rotation speed of the fertilizer discharging shaft, and that the installation position of the fertilizer apparatuses affected the uniformity of fertilization between the rows of the fertilizer applicator. The speed response test of the fertilizer discharging shaft showed that the average response time (Ts) of the fertilizer discharging shaft speed controlled by the self-calibration model was 0.40 s, the average steady-state error (ess) was 0.13 r/min, and the average overshoot (σ) was 7.33%. Compared with the original linear model, the ess was reduced by 0.23 r/min, and the σ was reduced by 1.54 percentage points. The results of the fertilization status detection tests showed that the system can achieve real-time detection of different operating parameters and states, as well as collaborative control of tractors and fertilizer applicators. The results of the fertilization rate control accuracy test showed that the average fertilization control error of the system was 1.91% under different target fertilization rate, which meets the requirements of variable-rate fertilization field operations. This study can serve as a technical reference for the design and development of fertilization robots in the context of unmanned farm development.

For the mechanized technical mode of total wheat straw returning to field, there are problems such as large vibration during the operation of the straw returning machine that, in turn, affect the effect of stubble breaking. This study took the Tongtian 1-JHY-220 straw returning machine as the research object to conduct field experiments, with wheat stubble height, forward velocity, and PTO speed as experimental parameters. And the vibration characteristics at different positions of the machine and the final stubble breaking rate were used as evaluation indicators. Combined with the orthogonal experiment and response surface analysis method, this article analyzes and discusses the influence of various parameters on vibration characteristics and operational effectiveness. The results show that PTO speed and wheat stubble height were the main factors affecting the vibration and operation quality of the straw returning machine. Low PTO speed and high stubble height can improve the stubble breaking rate of the straw returning machine and reduce its operation vibration. Furthermore, the multi-objective optimization results show that when the forward velocity in the range of 8.5–9 km/h, the PTO speed is 540 r/min, and the stubble height is in the range of 200–250 mm, the stubble breaking rate of the straw returning machine is greater than 86%. At this time, the total vibration of the straw returning machine and tractor rear axle is relatively small. This study can lay a foundation for further studying the impact of the vibration of the straw returning machine on the stubble breaking effect and provide a reference for the preparation of high-quality seedbed under conservation tillage.

Zinc finger proteins (ZFPs) are essential transcription factors in eukaryotes, particularly the extensively studied C2H2 family, which is known for its involvement in various biological processes. This research provides a thorough examination and analysis of the C2H2-ZFP gene family in Flammulina filiformis. Using bioinformatics tools, 58 FfC2H2-ZFP genes spread across 11 chromosomes were identified and scrutinized in detail for their gene structures, protein characteristics, and phylogenetic relationships. The study of phylogenetics and synteny sheds light on the evolutionary relationships among C2H2-ZFPs in F. filiformis and other fungi, revealing a complex evolutionary past. The identification of conserved cis-regulatory elements in the gene promoter regions suggests intricate functionalities, particularly in the developmental and stress response pathways. By utilizing RNA-seq and qRT-PCR techniques, the expression patterns of these genes were explored across different developmental stages and tissues of F. filiformis, unveiling distinct expression profiles. Notably, significant expression variations were observed in the stipe elongation region and pilei of various sizes, indicating potential roles in fruiting body morphogenesis. This study enhances our knowledge of the C2H2-ZFP gene family in F. filiformis and lays the groundwork for future investigations into their regulatory mechanisms and applications in fungal biology and biotechnology.

A good planting bed is a prerequisite for improving planting quality, while complex ground excitation often leads to machine bouncing and operation vibration, which then affects the operation effect. In order to improve the quality of rotary tillage operations, it is necessary to study the effects of various vibration excitations on the unit during tractor rotary tillage operations and analyze the vibration interaction relationship among the tractor, the three-point suspension mechanism, and the rotary tiller. For this purpose, multiple three-way acceleration sensors were installed at different positions on the rotary tiller unit of a Lexing LS1004 tractor(Lexing Agricultural Equipment Co. Ltd., Qingdao, China) to collect vibration data at different operating speeds and conduct vibration characteristic analysis between different components. The test results showed that when the unit moved forward at 2.1 km/h, 3.6 km/h, and 4.5 km/h, respectively, the vibration acceleration of the tractor, the three-point suspension mechanism, and the rotary tiller increased with the increase in speed, and there was indeed interaction between them. The vertical acceleration change during the test in the three-point suspension mechanism was the most significant (5.914 m/s2) and was related to the increase in the speed of the vehicle and the vibration transfer of the rotary tiller. Meanwhile, the vertical vibration acceleration of the tractor’s symmetrical structure was not similar, suggesting the existence of structural assembly problems. From the perspective of frequency domain analysis, the resonant frequency at the cab of the tractor was reduced in a vertical vibration environment, with relatively low frequencies (0~80 Hz) and small magnitudes, which might be beneficial to the driver’s health. The rotary tillage group resonated around 350 Hz, and this characteristic can be used to appropriately increase the vibration of the rotary tiller to reduce resistance. The tractor cab resonated around 280 Hz, which must be avoided during field operations to ensure driver health and reduce machine wear. The research results can provide a reference for reducing vibration and resistance during tractor rotary tillage operations, as well as optimizing and improving the structure of rotary tillers and tractors.

Coniella vitis is a dominant phytopathogen of grape white rot in China, significantly impacting grape yield and quality. Previous studies showed that the growth and pathogenicity of C. vitis were affected by the environmental pH. Arrestin-like protein PalF plays a key role in mediating the activation of an intracellular-signaling cascade in response to alkaline ambient. However, it remains unclear whether palF affects the growth, development, and virulence of C. vitis during the sensing of environmental pH changes. In this study, we identified a homologous gene of PalF/Rim8 in C. vitis and constructed CvpalF-silenced strains via RNA interference. CvpalF-silenced strains exhibited impaired fungal growth at neutral/alkaline pH, accompanied by reduced pathogenicity compared to the wild-type (WT) and empty vector control (CK) strains. The distance between the hyphal branches was significantly increased in the CvpalF-silenced strains. Additionally, CvpalF-silenced strains showed increased sensitivity to NaCl, H2O2, and Congo red, and decreased sensitive to CaSO4. RT-qPCR analysis demonstrated that the expression level of genes related to pectinase and cellulase were significantly down-regulated in CvpalF-silenced strains compared to WT and CK strains. Moreover, the expression of PacC, PalA/B/C/F/H/I was directly or indirectly affected by silencing CvpalF. Additionally, the expression of genes related to plant cell wall-degrading enzymes, which are key virulence factors for plant pathogenic fungi, was regulated by CvpalF. Our results indicate the important roles of CvpalF in growth, osmotolerance, and pathogenicity in C. vitis.

Most previously studies had considered that plant fungal disease spread widely and quickly by airborne fungi spore. However, little is known about the release dynamics, aerodynamic diameter, and pathogenicity threshold of fungi spore in air of the greenhouse environment. Grape gray mold is caused by Botrytis cinerea; the disease spreads in greenhouses by spores in the air and the spore attaches to the leaf and infects plant through the orifice. In this study, 120 μmol/L propidium monoazide (PMA) were suitable for treatment and quantitation viable spore by quantitative real-time PCR, with a limit detection of 8 spores/mL in spore suspension. In total, 93 strains of B. cinerea with high pathogenicity were isolated and identified from the air samples of grapevines greenhouses by a portable sampler. The particle size of B. cinerea aerosol ranged predominately from 0.65–3.3 μm, accounting for 71.77% of the total amount. The B. cinerea spore aerosols were infective to healthy grape plants, with the lowest concentration that could cause disease being 42 spores/m3. Botrytis cinerea spores collected form six greenhouse in Shandong Province were quantified by PMA-qPCR, with a higher concentration (1182.89 spores/m3) in May and June and a lower concentration in July and August (6.30 spores/m3). This study suggested that spore dispersal in aerosol is an important route for the epidemiology of plant fungal disease, and these data will contribute to the development of new strategies for the effective alleviation and control of plant diseases.

IntroductionThe Songnen Plain is one of the three major saline-alkali areas in China, covering a vast area, where drought and overgrazing have exacerbated the salinization trend, and will have great potential for development if utilized rationally. Phosphorus, as one of important soil nutrients, plays a crucial role in plant growth. How to minimize its loss and migration has become a current research hotspot. The objective of the present study was to elucidate the adsorption properties of phosphorus in soils affected by salinization and to establish the correlation between the potential for phosphorus release and soil properties.MethodsA batch treatment test was conducted in this study using three soils with the various salinization degrees to examine the impact of environmental factors on the adsorption properties and potential release of phosphorus.Results and discussionIt was found that the maximum phosphorus adsorption by the three salinization soils in 0-360 minutes accounted for 86.8%-90.5% of the total adsorption capacity; the equilibrium adsorption capacity was: HS> MS> LS. In cases where the phosphorus level in the surrounding liquid is low, the three levels of salinized soils exhibited varying levels of phosphorus discharge, with the adsorbent acting as the origin of contaminants. The Pseudo-second-order model kinetics and Langmuir equation can well describe the adsorption process, and the adsorption process is spontaneous heat absorption with entropy increase. Increasing the pH led to an increase in the adsorption of phosphorus from the three salinized soils. Additionally, the adsorption was enhanced by introducing varying concentrations of Na+, Ca2+, and Al3+ to the background solution. The phosphorus eutrophication release risk (ERI) demonstrated a gradual decline as temperature increased. Correlation analysis revealed a noteworthy positive correlation between TN, TP, and ERI, as well as a significant negative correlation between CEC, K+, and ERI. Furthermore, there was a highly significant negative correlation between coarse silt and fine silt. Considering local climatic and environmental factors is crucial for controlling the adsorption capacity of phosphorus in various salinized soils, as it can unveil the mechanism of phosphorus adsorption and impact its migration and release risk.

Based on the observational data of precipitation at the stations of Zhejiang, relative bias(RB), root mean square error(RMSE), and correlation coefficient (CC) were used to evaluate the quasi-real-time product IMERG_ER V06B of GPM satellite during the landing periods of typhoon Chan-hom, Yagi, Lekima, Mitag, and Hagupit during 2015 to 2020. POD, FAR and CSI were used to evaluate the ability of precipitation retrieval of IMERG at different precipitation levels. The results show that the IMERG precipitation data of GPM can reflect the spatial distribution characteristics of precipitation, but its ability to capture the range of heavy precipitation is still insufficient. Precipitation during typhoon Yagi, Lekima, Mitag, and Hagupit were overestimated, while precipitation during typhoon Chan-hom were underestimated. IMERG has the ability to capture the change of precipitation of typhoon Hagupit, followed by typhoon Chan-hom, Yagi, and Lekima, and typhoon Mitag is the worst. According to the results of classification test, POD and CSI decrease, while FAR increases with the increase of precipitation, which indicates that IMERG has the best ability to identify light rain, but poor ability to capture heavy rain. Meanwhile, IMERG overestimated the possibility of heavy precipitation. Objectively, precipitation product of IMERG_ER V06B is insufficient to capture the precipitation intensity of typhoon, and the quantitative error of individual typhoon is large. Overall, it has a fair ability to capture the number and variation of peak and valley of typhoon rainfall, which can be applied to estimate the change of typhoon rainfall in complex terrain, due to its similar if not better ability of retrieving precipitation in mountainous area as in plain area.

Nucleotide substitutions have played an important role in molecular evolution, and understanding their dynamics would contribute to genetic studies. Related research with defined DNA sequences lasted for decades until whole-genome sequencing arose. UV radiation (UVR) can generate base changes and other genetic variations in a short period of time, so it would be more meaningful to explore mutations caused by UVR from a genomic perspective. The monokaryon enoki strain WT583 was selected as the experimental material in this study because it can spontaneously produce large amounts of oidia on PDA plates, and the monokaryons originating from oidia have the same genotype as their mother monokaryon. After exposure to UV radiation, 100 randomly selected mutants, with WT583 as the reference genome, were sent for genome sequencing. BWA, samtools, and GATK software were employed for SNP calling, and the R package CMplot was used to visualize the distribution of the SNPs on the contigs of the reference genome. Furthermore, a k-mer-based method was used to detect DNA fragment deletion. Moreover, the non-synonymous genes were functionally annotated. A total of 3707 single-base substitutions and 228 tandem mutations were analyzed. The immediate adjacent bases showed different effects on the mutation frequencies of adenine and cytosine. For adenine, the overall effects of the immediate 5′-side and 3′-side bases were T > A > C > G and A > T > G > C, respectively; for cytosine, the overall effects of the immediate 5′-side and 3′-side bases were T > C > A > G and C > T > A > G, respectively. Regarding tandem mutations, the mutation frequencies of double-transition, double-transversion, 3′-side transition, and 5′-side transition were 131, 8, 72, and 17, respectively. Transitions at the 3′-side with a high mutation frequency shared a common feature, where they held transversions at the 5′-side of A→T or T→A without covalent bond changes, suggesting that the sequence context of tandem motifs might be related to their mutation frequency. In total, 3707 mutation sites were non-randomly distributed on the contigs of the reference genome. In addition, pyrimidines at the 3′-side of adenine promoted its transversion frequency, and UVR generated DNA fragment deletions over 200 bp with a low frequency in the enoki genome. The functional annotation of the genes with non-synonymous mutation indicated that UVR could produce abundant mutations in a short period of time.

Many lines of evidence demonstrate the associations of colorectal cancer (CRC) with intestinal microbial dysbiosis. Recent reports have suggested that maintaining the homeostasis of microbiota and host might be beneficial to CRC patients, but the underlying mechanisms remain unclear. In this study, we established a CRC mouse model of microbial dysbiosis and evaluated the effects of fecal microbiota transplantation (FMT) on CRC progression. Azomethane and dextran sodium sulfate were used to induce CRC and microbial dysbiosis in mice. Intestinal microbes from healthy mice were transferred to CRC mice by enema. The vastly disordered gut microbiota of CRC mice was largely reversed by FMT. Intestinal microbiota from normal mice effectively suppressed cancer progression as assessed by measuring the diameter and number of cancerous foci and significantly prolonged survival of the CRC mice. In the intestine of mice that had received FMT, there were massive infiltration of immune cells, including CD8+ T and CD49b+ NK, which is able to directly kill cancer cells. Moreover, the accumulation of immunosuppressive cells, Foxp3+ Treg cells, seen in the CRC mice was much reduced after FMT. Additionally, FMT regulated the expressions of inflammatory cytokines in CRC mice, including down-regulation of IL1a, IL6, IL12a, IL12b, IL17a, and elevation of IL10. These cytokines were positively correlated with Azospirillum_sp._47_25, Clostridium_sensu_stricto_1, the E. coli complex, Akkermansia, Turicibacter, and negatively correlated with Muribaculum, Anaeroplasma, Candidatus_Arthromitus, and Candidatus Saccharimonas. Furthermore, the repressed expressions of TGFb, STAT3 and elevated expressions of TNFa, IFNg, CXCR4 together promoted the anti-cancer efficacy. Their expressions were positively correlated with Odoribacter, Lachnospiraceae-UCG-006, Desulfovibrio, and negatively correlated with Alloprevotella, Ruminococcaceae UCG-014, Ruminiclostridium, Prevotellaceae UCG-001 and Oscillibacter. Our studies indicate that FMT inhibits the development of CRC by reversing gut microbial disorder, ameliorating excessive intestinal inflammation and cooperating with anti-cancer immune responses.

Mushrooms of the edible and medicinal which are highly nutritious and environmentally friendly crops carry numerous medicinal benefits. For the abundant and high diversity of bioactive metabolites they possess, which are considered to be an important pool of bioresources. The efficient breeding technique is always a challenging task in mushrooms for obtaining better character strains, which are essential for developing healthy products and even consumption. This review comprehensively summarizes the breeding techniques applied to the edible and medicinal mushrooms. Including the traditional mutagenesis method, and even modern gene-editing breeding techniques, the effects of each method, and the comparison of each breeding technique are systematic illustrations. Strategies for mushroom breeding techniques in the future are also discussed in this review paper. With the ongoing sequencing of the mushroom genome, knowledge of the gene background of the strains and functions can be available for developing better markers for gene-editing breeding as CRISPR/Cas9 systems. Combine the metabolism engineering and in-silico tools analysis was the rational design of the novel strains. Modern physical mutagenesis techniques such as the ARTP and the combination of the other physical, and chemical breeding mutagens with cross-breeding techniques or the protoplasts fusion will also lead to superior strains for cultivation and pave the way for higher quality and yield.

There is shallow sea sandy debris flow in Ying Ⅱ Member in Dongfang 1-1 Gas Field, Yinggehai Basin.Based on the detailed description of cores, combined with well logs, seismic section, geochemical experiments and the research progress of gravity flow, the core, logging and seismic facies characteristics of shallow sea sandy debris flow in the study area are determined.By analyzing the sedimentary background of the study area, the sedimentary mechanism of shallow sea sandy clastic flow in ⅡL and ⅢU gas groups of Ying Ⅱ Member in Dongfang Gas Field is further discussed.It is found that it is characterized massive sandstone rich in mudstone floating debris and "mud-coated intraclasts", which are typical sandy clastic flow characteristics, in core facies, funnel type and bell-funnel type which jugged acutely in logging facies, medium amplitude parallel sheet reflection and low frequency parallel sub parallel blank reflection in seismic facies, sheet and flat lenticular distribution on the section.The results show that the sandy debris flow in the study area is the sedimentary mechanism of the shallow sea sandy debris flow formed by the combined action of the west delta front sediment collapse and re-transportation caused by the storm and the Red River flood gravity flow on the north side.

ObjectiveTo evaluate the feasibility, safety, and outcomes of percutaneous endoscopic transforaminal discectomy (PETD) for thoracolumbar junction disc herniation (TLDH) with or without calcification.MethodsThis study included 12 patients diagnosed with TLDH with or without calcification who met the inclusion criteria and underwent surgery for PETD from January 2019 to December 2021. The mean patient age, operation time, hospitalization time, time in bed, and complications were recorded. Patients were followed up for at least 9 months. Visual analog scale (VAS) scores for low-back and leg or thoracic radicular pain and modified Japanese Orthopedic Association score (m-JOA) scores were preoperatively evaluated, at 1 day and 3, 6, and 12 months postoperatively or at last follow-up. The modified MacNab criteria were used to evaluate clinical efficacy at 12 months postoperatively or at last follow-up.ResultsThe mean patient age, operation time, hospitalization time, and time in bed were 53 ± 13.9 years, 101.3 ± 9.2 min, 4.5 ± 1.3 days, and 18.0 ± 7.0 h, respectively. The mean VAS scores of low-back and leg or thoracic radicular pain improved from 5.8 ± 1.5 and 6.5 ± 1.4 to 2.0 ± 0.9 and 1.3 ± 0.5, respectively (P

The goals of this work were to screen physiological and biochemical indexes to assess a set of V. vinifera germplasm resources, to compare evaluation methods for cold hardiness, and to establish a comprehensive method that can be used for more accurate screening for cold hardiness in V. vinifera. Four single methods were used to evaluate the cold hardiness of 20 germplasms resources and 18 physiological and biochemical indexes related to cold hardiness were determined. The LT50 values determined by electrical conductivity (EL), 2,3,5-triphenyltetrazolium chloride staining (TTC), differential thermal analysis (DTA), and recovery growth (RG) methods showed extremely significant positive correlation. Bound water content (BW), proline content (Pro), total soluble sugar content (TSS), malondialdehyde content (MDA), catalase content (CAT), and ascorbic acid content (ASA) exhibited significant correlation with LT50 values measured by different evaluation methods. The comprehensive cold hardiness index calculated by principal component analysis (PCA) combined with subordinate function (SF) was negatively correlated with LT50 values measured by different evaluation methods. Meili and Ecolly exhibited the highest cold hardiness, indicating their potential for use as parents for cold hardiness breeding. EL, DTA, TTC, and RG methods successfully distinguished cold hardiness among different V. vinifera germplasm lines. Measurements of BW, Pro, TSS, MDA, CAT, and ASA in dormant shoots also can be used as main physiological and biochemical indexes related to cold hardiness of V. vinifera. Comprehensive evaluation by PCA combined with SF can accurately screen cold hardiness in V. vinifera. This study provides a reference and accurate identification method for the selection of cold hardiness parents and the evaluation of cold hardiness of germplasm of V. vinifera.

Most cultivars of Vitis vinifera L. are very sensitive to cold. As an exogenous protectant, Biodegradable Liquid Film (BLF) is considered to protect winegrapes from low temperatures and dry winds for safe overwintering. This study aimed to reveal the physiological and biochemical mechanisms of BLF regulating the freezing tolerance of wine grapes. Groups of ten-year-old vines (Cabernet Sauvignon) were sprayed with BLF in November 2020 and 2021, or left untreated as a control treatment, and field plant mortality after overwintering were investigated. Branch samples were collected monthly for determination of biochemical indicators. Dormant two-year-old cuttings (Cabernet Sauvignon) were also used for the determination of relative expression levels of key genes. The results showed that the application of BLF reduced the branch semi-lethal temperature in January and February samples compared with control, and reduced the mortality of above-ground parts, branches and buds. The physiological status of shoots was greatly affected by the climatic conditions of the year, but BLF treatment increased the levels of soluble protein and soluble sugar, and also decreased the content of superoxide anion and malondialdehyde at most sampling times. Correlation analysis showed that the differences in freezing tolerance between BLF and no treated overwintering(CK) vines were mainly related to peroxidase activity, soluble sugar, reducing sugar and starch content. Low temperature stress activated the over expression of ICE1, CBF1, and CBF3, especially for 12h. BLF treatment significantly increased the expression levels of CBF1 and CBF3 under low temperature stress. Overall, these results demonstrate that BLF treatment protects vines from freezing damage by upregulating osmo-regulatory substances and alleviating oxidative damage.

Low temperatures are among the most important abiotic stresses, severely affecting vine growth and development. In order to overwinter safely, grape growers pay high labor costs, which are unsustainable in modern agriculture. Biodegradable liquid film (BLF) is an environmentally friendly material that assists grapevines to withstand the cold. In this experiment, Cabernet Sauvignon from Junding Winery was used as material for field trials. The regulatory effect of BLF on the cold resistance of grapevines was analyzed, and its mechanism of action was explored using metabolomics. The results showed that BLF significantly increased the activity of superoxide dismutase, the content of proline and ascorbic acid, reducing sugar, sucrose, and soluble sugar, and also decreased the content of malondialdehyde. In addition, BLF significantly increased the activity of invertase and decreased the activity of pectinase and phosphofructokinase. Metabolomics data showed that BLF improved cold resistance in grapevines by affecting lipids, flavonoids, amino acids, and nucleotide metabolic pathways.

Traffic flow forecasting is an important function of intelligent transportation systems. With the rise of deep learning, building traffic flow prediction models based on deep neural networks has become a current research hotspot. Most of the current traffic flow prediction methods are designed from the perspective of model architectures, using only the traffic features of future moments as supervision signals to guide the models to learn the spatiotemporal dependence in traffic flow. However, traffic flow data themselves contain rich spatiotemporal features, and it is feasible to obtain additional self-supervised signals from the data to assist the model to further explore the underlying spatiotemporal dependence. Therefore, we propose a self-supervised traffic flow prediction method based on a spatiotemporal masking strategy. A framework consisting of symmetric backbone models with asymmetric task heads were applied to learn both prediction and spatiotemporal context features. Specifically, a spatiotemporal context mask reconstruction task was designed to force the model to reconstruct the masked features via spatiotemporal context information, so as to assist the model to better understand the spatiotemporal contextual associations in the data. In order to avoid the model simply making inferences based on the local smoothness in the data without truly learning the spatiotemporal dependence, we performed a temporal shift operation on the features to be reconstructed. The experimental results showed that the model based on the spatiotemporal context masking strategy achieved an average prediction performance improvement of 1.56% and a maximum of 7.72% for longer prediction horizons of more than 30 min compared with the backbone models.

Abstract Passionfruit (Passiflora edulis) is widely cultivated in tropical and subtropical regions around the world. Several viruses of the genus Potyvirus pose serious threat to passion fruit production. The origin, dispersal and evolution of these potyviruses, however, are poorly understood. Here, we investigated the genetic structure of telosma mosaic virus (TelMV), a potyvirus that infects passionfruit in East and Southeast Asia, after a survey of its incidence in passionfruit plants of China. The phylogeny inferred from 140 nucleotide sequences of the coat protein (CP) gene of TelMV, including 96 determined in this study, separated this virus into 4 clades. TelMV isolates from passionfruit were placed into Clade 1–3, while those from other plant species into Clade 4. Interestingly, TelMV isolates of passionfruit from Thailand were found in all the three clades of Clade 1–3, but those from China and Vietnam were found exclusively in Clade 1. Nevertheless, TelMV isolates within Clade 1 tended to cluster according to their geographical origin. Geographical populations from Thailand, Taiwan and Hainan islands of China showed significant genetic differences with one another and with those from Guangxi, Fujian, Guangdong, Yunnan and Jiangsu provinces of China. Altogether, these data suggest that several distinct TelMV clades had arisen from the passionfruit of Thailand, but only one of which was dispersed. In expanding its distribution, this clade of TelMV has undergone geography-associated evolution. Further studies on this hypothesis may shed new insights into mechanisms underlying the emergence of potyviral diseases in passionfruit plants.

Cholestasis is the most destructive pathological manifestation of liver disease and available treatments are very limited. Paeoniae Radix Rubra (PRR) is an important traditional Chinese drug used to treat cholestasis. This study combined targeted metabonomics, PCR array analysis, and 16S rRNA sequencing analysis to further clarify the mechanisms of PRR in the treatment of cholestasis. PRR conspicuously reversed the elevation of fatty acids (FFA 14:0 and other 14 fatty acids) and the decrease of organic acids (pyruvic acid and citric acid) in a cholestatic model induced by α-naphthyl isothiocyanate (ANIT). Eight elevated amino acids (L-proline, etc.) and five elevated secondary bile acids (taurohyodeoxycholic acid, etc.) in model rats were also reduced by PRR. Pathway analysis revealed that PRR significantly alleviated eight pathways (β-alanine metabolism). Furthermore, we found that PRR significantly reversed the decrease of Cpt1a, Hadha, Ppara, and Slc25a20 (four genes relevant to fatty acid β-oxidation) mRNAs caused by ANIT, and PRR conspicuously decreased nine acylcarnitines (the forms of fatty acids into mitochondria for β-oxidation) that increased in model rats. These results indicate that PRR could enhance fatty acid β-oxidation, which may be the way for PRR to reduce the levels of 15 fatty acids in the serum of model rats. 16S rRNA sequencing analysis revealed that PRR alleviated gut microbiota disorders in model rats, including upregulating four genera (Coprococcus, Lactobacillus, etc.) and downregulating four genera (Bacteroides, Escherichia, etc.). As the relative abundance of these eight genera was significantly correlated with the levels of the five secondary bile acids (deoxycholic acid, taurolithocholic acid, etc.) reduced by PRR, and Bacteroides and Escherichia were reported to promote the production of secondary bile acid, we inferred that the downregulation of PRR on five secondary bile acids in model rats was inseparable from gut microbiota. Thus, the gut microbiota also might be a potential pharmacological target for the anticholestatic activity of PRR. In conclusion, we consider that the mechanisms of PRR in treating cholestasis include enhancing fatty acid β-oxidation and alleviating gut microbiota disorders.

Exosomes, as natural biomolecular carriers produced by cells, have the potential and advantage of delivering drugs to target organs or cells in vivo. The steps to improve exosomes as a drug delivery system can be divided into three steps:large-scale preparation of exosomes, loading of drugs and targeted delivery of exosomes. Based on the existing production process and technology, there is still much room for improvement. This review highlights the research progress in three aspects and proposes new technologies and innovative approaches to improve the efficiency of exosome delivery.

The correlations were explored between fertility indicators of intraspecific V. vinifera hybrids and different cultivars were subjected to selfing or used in reciprocal crosses by testing them as female parents or male parents. Two cold-resistant and four high-quality cultivars were selected, and the offspring of fourteen crosses and six self-combinations were evaluated. The pollen viability of the six cultivars was determined by TTC staining. Compatibility and the rates of fruit-setting, seediness, germination, emergence, and seedling were measured as parameters that can affect fertility of both hybridization and self-crossing processes. Using principal component analysis, the six fertility indexes were transformed into comprehensive principal components, and the weights of the indexes were determined. Combined with the membership function method, the fertility index was comprehensively evaluated for different crosses to screen for hybrid combinations with higher fertility. The results showed a high positive correlation between the pollen viability of the cultivar subjected to selfing and the fruit-setting rate, seediness rate, and hybrid compatibility index of the cultivar used as the male parent for crossing. Additionally, there was a one-to-one positive correlation between the fruit-setting rate, germination rate, emergence rate, and seedling rate of the selfed cultivar and the fruit-setting rate, germination rate, and seedling rate of the cultivar used as the female parent for crossing. There was some variation in the comprehensive fertility index values for the parents and combinations in different years. The comprehensive fertility index was always the highest for Ecolly as the male parent. The composite fertility index values were relatively high when Dunkelfelder, Cabernet Sauvignon, or Marselan were used as the female parent. The combinations of C1 (Cabernet Sauvignon × Ecolly), C3 (Marselan × Ecolly) and C6 (Dunkelfelder × Ecolly) exhibited relatively high comprehensive fertility indices, and pedigree clustering shows that these three combinations cluster into one class of highly fertile hybrid combinations. This study provides the basis for effective intraspecific hybrid breeding of grape (V. vinifera).

Organic contaminants such as dyes and antibiotics have become the focus of water treatment research in recent years due to their complex composition, high toxicity, and difficulty in biodegradation. Spinel ferrite heterogeneous Fenton-like catalysts, with a chemical formula of MFe2O4 (MFe2O4, M is a divalent metallic cation or its combination, and the divalent cation is generally Ni, Zn, Mn, Co, Cu, and Mg, etc.), have attracted much attention because of their excellent structural stability and good magnetic recovery performance. However, the catalytic activity of these catalysts is not ideal and almost all the reported catalysts are synthesized by pure chemical reagents, which restrict their industrial application. Therefore, the preparation of highly efficient heterogeneous Fenton-like catalysts with low cost becomes the key to the treatment of refractory organic wastewater. In this study, copper-doped spinel ferrite (Ni, Mg, Cu)Fe2O4 was successfully synthesized from nickel sulfide concentrate by a coprecipitation–calcination method. The effect of copper doping concentration on the structure, micro-morphology, and catalytic performance of as-prepared samples was systematically investigated by X-ray diffraction, scanning electron microscopy, and X-ray photoelectron spectroscopy. The optimal catalytic system was established as the photo-assisted Fenton-like catalytic system, “(Ni, Mg, Cu)Fe2O4 catalyst/H2O2/visible light”, and the enhancement mechanism of copper doping on the catalytic activity of (Mg, Ni)Fe2O4 was revealed. Results showed that all formed products were pure spinel ferrites under the selected synthesis conditions. With 1∶1 molar ratio of Ni to Cu, the formed (Ni, Mg, Cu)Fe2O4 catalyst achieved 94.5% degradation efficiency for 10-mg∙L−1 RhB solution under visible light irradiation for 180 min. This observed behavior may be ascribed mainly to the increased relative contents of Fe3+ and Cu2+ ions at octahedral site. Hydroxyl radical (·OH) reaction accelerated due to increased amount of Fe3+ and Cu2+ exposed on the surface and enhanced synergetic effect between Fe3+ and Cu2+. This improved the degradation efficiency of RhB solution from 73.1% to 94.5%.

Controlled deposition of droplets on super-repellent surfaces is a challenging task in many practical applications. Han et al. show that already a rather thin encapsulating film of a highly viscous liquid can fine tune droplet impact as to suppress both rebound and splash.

We propose a novel approach to cluster hierarchical time series (HTS) for efficient forecasting and data analysis. Inspired by a practically important but unstudied problem, we found that leveraging local information when clustering HTS leads to a better performance. The clustering procedure we proposed can cope with massive HTS with arbitrary lengths and structures. In addition to providing better insights, this method can also speed up the forecasting process for a large number of HTS. Each time series is first assigned the forecast from its cluster representative, which can be considered as “prior shrinkage” for the set of time series it represents. Then, the base forecast can be efficiently adjusted to accommodate the specific attributes of the time series. We empirically show that our method substantially improves performance for large-scale clustering and forecasting tasks involving HTS.

China is one of the major antibiotic producing and consuming countries in the world and demand for and output of antibiotics are increasing year by year. The wastewater produced during the production of antibiotics is of complex composition, high chemical oxygen demand, high concentrations of toxic and harmful substances and strong resistance to biodegradation. This is problematic for the process of pharmaceutical wastewater treatment. Additionally, due to the extensive use of antibiotics, a variety of antibiotics are constantly released into the environment, with adverse effects on aquatic organisms. Although antibiotic concentrations in wastewater are low, the accumulation of low-dose, long-duration antibiotics can lead to the development of drug-resistant strains that threaten human health and the entire ecosystem. Therefore, how to effectively remove antibiotic residues in water is an important challenge toward ensuring the safety of water quality, the environment, and ecology. Advanced oxidation technology (AOP), which has an extremely high oxidation potential, can generate hydroxyl radicals in the reaction process, and can degrade organic compounds rapidly without secondary pollution. Therefore, it exhibits clear advantages in the treatment of antibiotics in water. As a kind of AOP, homogeneous Fenton oxidation technology (Fe2+/H2O2 system) has attracted considerable attention owing to its rapid reaction, simplicity, and high degradation efficiency. Heterogeneous Fenton-like oxidation technology using an iron-based solid catalyst instead of Fe2+ ions can effectively reduce the formation of iron-containing sludge and broaden the pH reaction range to overcome the shortcomings of Homogeneous Fenton. Moreover, recycling of the catalyst has been developed rapidly in recent years and has achieved ideal results when applied to the degradation of antibiotics. In this paper, research progress in heterogeneous Fenton-like catalysts for degradation of antibiotics was reviewed. Based on the core issues of heterogeneous Fenton-like catalysts, the methods, measures, and new viewpoints for improving catalytic performance were expounded upon. Aiming at the problems of heterogeneous Fenton-like technology in degrading antibiotics, future development directions were presented.

Kaolin particle film (KPF) is an aqueous formulation of chemically inert mineral particles that can be sprayed on the surface of crops to form a protective film, resulting in increased fruit yield and quality. In this work, the effects of kaolin-based, foliar reflectant particle film on grape composition and volatile compounds in ‘Meili’ (Vitis vinifera L.) grapes were investigated under different growth stages over two growing seasons. The 100-berry weight and titratable acid content were decreased, and the sugar and soluble solid contents were increased in grapes of plants treated with kaolin over 2 years. Compared with grapes from plants not sprayed with kaolin, the levels of total phenol, flavonoid, flavanol, tannin, and anthocyanins of grapes from plants treated with kaolin for 2 years were mostly increased. High-performance liquid chromatography (HPLC) analysis also revealed an increased content of monomeric anthocyanin and changed anthocyanin composition. However, there was little effect on the volatile compounds in the grapes. These results demonstrate that KPF can facilitate the accumulation of sugar and phenolics, thereby improving grape quality even in a humid climate.

Electronic Health Records (EHR) are subject to noise, biases and missing data. Here, the authors present MixEHR, a multi-view Bayesian framework related to collaborative filtering and latent topic models for EHR data integration and modeling.

In this study, different contents (0.0, 0.1, 0.2, and 0.4 wt.%) of Zr55Cu30Al10Ni5 amorphous alloys (Zr-AA) are used to manipulate hypereutectic Al-Si alloys. The edge-to-edge model shows that there is a perfect lattice matching between the crystallized phases and α-Al and Si phases. The thermal analysis of solidification behavior of hypereutectic Al-Si alloys unmanipulated and manipulated by Zr-AA, indicates that the addition of Zr-AA greatly promotes the nucleation of primary Si and significantly accelerates the process of eutectic reaction. When manipulated by the optimal content of 0.2 wt.% Zr-AA, the sizes of α-Al dendrites and primary Si decrease from 212.46 μm and 19.24 μm to 143.39 μm and 16.47 μm, respectively. The eutectic Si changes from needle-like to rod-like and exhibits a mean size decrease from 20.21 μm to 11.69 μm. Meanwhile, alloys exhibit ultimate tensile strength of 362 MPa and fraction strain of 2.21% after 0.2 wt.% Zr-AA manipulation, which are 23% and 37% higher than those of the unmanipulated alloy (295 MPa and 1.61%). The strengthening mechanisms of hypereutectic Al-Si alloys manipulated by in situ nanocrystals crystallized in the melt are mainly attributed to the fine grain strengthening effect and precipitation strengthening effect. The increase in plasticity is due to the refinement and morphology improvement of Si phases.

Abstract Background Skin wounding is very common and may be slow to heal. Increasing evidence shows that exosomes derived from mesenchymal stem cells (MSCs) dramatically enhance skin wound healing in a paracrine manner. However, the mechanism underlying this phenomenon has not yet been elucidated. Thus, the objective of the present study was to identify the signaling pathways and paracrine factors by which MSC-derived exosomes promote de novo skin tissue regeneration in response to wound healing. Methods In vitro and in vivo skin wound healing models were created by treating immortalized human keratinocytes (HaCaT) with hydrogen peroxide (H2O2) and excising full-thickness mouse skin, respectively. Exosomes were extracted from human umbilical cord Wharton’s jelly MSCs (hucMSC-Ex) by ultracentrifugation of cell culture supernatant. Results The hucMSC-Ex treatment significantly increased HaCaT cell proliferation and migration in a time- and dose-dependent manner, suppressed HaCaT apoptosis induced with H2O2 by inhibiting nuclear translocation of apoptosis-inducing factor (AIF) and upregulating poly ADP ribose polymerase 1 (PARP-1) and poly (ADP-ribose) (PAR). The animal experiments showed that relative to hucMSCs, hucMSC-Ex attenuated full-thickness skin wounding by enhancing epidermal re-epithelialization and dermal angiogenesis. Conclusions These findings indicated that direct administration of hucMSC-Ex may effectively treat cutaneous wounding and could be of great value in clinical settings.

Traffic flow forecasting is a basic function of intelligent transportation systems, and the accuracy of prediction is of great significance for traffic management and urban planning. The main difficulty of traffic flow predictions is that there is complex underlying spatiotemporal dependence in traffic flow; thus, the existing spatiotemporal graph neural network (STGNN) models need to model both temporal dependence and spatial dependence. Graph neural networks (GNNs) are adopted to capture the spatial dependence in traffic flow, which can model the symmetric or asymmetric spatial relations between nodes in the traffic network. The transmission process of traffic features in GNNs is guided by the node-to-node relationship (e.g., adjacency or spatial distance) between nodes, ignoring the spatial dependence caused by local topological constraints in the road network. To further consider the influence of local topology on the spatial dependence of road networks, in this paper, we introduce Ollivier–Ricci curvature information between connected edges in the road network, which is based on optimal transport theory and makes comprehensive use of the neighborhood-to-neighborhood relationship to guide the transmission process of traffic features between nodes in STGNNs. Experiments on real-world traffic datasets show that the models with Ollivier–Ricci curvature information outperforms those based on only node-to-node relationships between nodes by ten percent on average in the RMSE metric. This study indicates that by utilizing complex topological features in road networks, spatial dependence can be captured more sufficiently, further improving the predictive ability of traffic forecasting models.

Downy mildew is a major threat to viticulture, leading to severe yield loss. The use of traditional copper-based fungicides is effective, but has adverse effects on the environment and human health, making it urgent to develop an environmentally friendly disease management program. Multi-functional kaolin particle film (KPF) is promising as an effective and safer treatment strategy, since this material lacks chemically active ingredients. In this study, ability of Kaolin particle film (KPF) pretreatment to protect grapevine leaves from Plasmopara viticola was tested and the mode of action of KPF was analyzed. KPF application reduced the disease severity and the development of intercellular hyphae. Additionally, there was reduced accumulation of H2O2 and malondialdehyde (MDA) with pretreatment. The observation of ultrastructure on the leaf surface showed KPF deposition and stomatal obstruction, indicating that KPF protected plants against disease by preventing the adhesion of pathogens to the leaf surface and blocking invasion through the stomata. KPF pretreatment also activated host defense responses, as evidenced by increased activities of anti-oxidative enzymes [superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)] and defense-related enzymes [phenylalanine ammonia-lyase (PAL), chitinases, and β-1,3-glucanases], increased phytohormone signals [abscisic acid (ABA), salicylic acid (SA), and jasmonic acid (JA)] and the up-regulation of defense genes related to plant defense. Overall, these results demonstrate that KPF treatment counters grapevine downy mildew by protecting leaves and enhancing plant defense responses.

A super-hydrophobic anti-corrosion film was facilely prepared via in situ growth of layered double hydroxides (LDHs) on the etched AZ31 magnesium alloy and then modification by 1H, 1H, 2H, 2H-perfluorooctyltrimethoxysilane (PFOTMS) in this work. The morphology, structure, composition, surface roughness and water contact angles (WCA), and the anti-corrosion performance of the samples were investigated. The results revealed that the micro/nano hierarchical surface morphology of the films was composed of island structures obtained after chemical etching and MgAl-LDH nanowalls grown in situ. The best hydrophobicity (CA = 163°) was obtained on the MgAl-LDHs with the maximum surface roughness. Additionally, the potentiodynamic polarization, electrochemical impedance spectroscopy, and immersion test indicated that the super-hydrophobic LDH films provided better corrosion resistance to AZ31 magnesium alloy due to the double-protection derived from the LDHs and super-hydrophobic properties. Furthermore, the contact angle could be kept at above 140° after dipped in 3.5 wt% NaCl solution for 6 days.

Tissues and organs undergo structural deterioration and functional decline during aging. DNA damage is considered a major cause of stem cell senescence. Although stem cells develop sophisticated DNA repair systems, when the intrinsic and extrinsic insults exceed the DNA repair capacity, cellular senescence, and age-related diseases inevitably occur. Therefore, the prevention and alleviation of DNA damage is an alternative to DNA repair in attenuating stem cell senescence and preventing age-related diseases. Pre-B-cell leukaemia homeobox 1 (PBX1) participates in maintaining the pluripotency of human embryonic and haematopoietic stem cells. Our recent studies showed that PBX1 promotes hair follicle-derived mesenchymal stem cell (HF-MSC) proliferation, decreases cellular senescence and apoptosis, and enhances induced pluripotent stem cell generation. Whether PBX1 attenuates HF-MSC senescence and apoptosis by alleviating DNA damage or by enhancing DNA repair remains unknown. In this study, we aimed to determine the effects of PBX1 on the intrinsic ROS or extrinsic H2O2-induced cellular senescence of HF-MSCs. To this end, we generated HF-MSCs overexpressing either PBX1, or poly (ADP-ribose) polymerase 1, or both. Our results showed that PBX1 overexpression attenuates HF-MSC senescence and apoptosis by alleviating reactive oxygen species (ROS)-mediated DNA damage instead of enhancing DNA repair. This is the first study to report that PBX1 attenuates stem cell senescence and apoptosis by alleviating DNA damage. It provides new insight into the mechanism of stem cell senescence and lays the foundation for the development of strategies for age-related disease prevention and treatment, and in particular, hair follicle repair and regeneration.

Given the limited power of neuropsychological tests, there is a need for a simple, reliable means, such as gait, to identify mild dementia and its subtypes. However, gait characteristics of patients with post-stroke dementia (PSD) and Alzheimer’s disease (AD) are unclear. We sought to describe their gait signatures and to explore gait parameters distinguishing PSD from post-stroke non-dementia (PSND) and patients with AD. We divided 3-month post-stroke patients into PSND and PSD groups based on the Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), and the activity of daily living (ADL). Thirty-one patients with AD and thirty-two healthy controls (HCs) were also recruited. Ten gait parameters in one single and two dual-task gait tests (counting-backward or naming-animals while walking) were compared among the groups, with adjustment for baseline demographic covariates and the MMSE score. The area under the receiver operating characteristic curve (AUC) was used to identify parameters discriminating PSD from individuals with PSND and AD. Patients with PSD and patients with AD showed impaired stride length, velocity, stride time, and cadence while patients with PSD had altered stance and swing phase proportions (all p ≤ 0.01, post hoc). Patients with AD had smaller toe-off (ToA) and heel-to-ground angles (HtA) (p ≤ 0.01) than HCs in dual-task gait tests. Individuals with PSD had a shorter stride length, slower velocity, and altered stance and swing phase percentages in all tests (p ≤ 0.01), but a higher coefficient of variation of stride length (CoVSL) and time (CoVST) only in the naming animals-task gait test (p ≤ 0.001) than individuals with PSND. ToA and HtA in the naming animals-task gait test were smaller in individuals with AD than those with PSD (p ≤ 0.01). Statistical significance persisted after adjusting for demographic covariates, but not for MMSE. The pace and the percentage of stance or swing phase in all tests, CoVST in the dual-task paradigm, and CoVSL only in the naming animals-task gait test (moderate accuracy, AUC > 0.700, p ≤ 0.01) could distinguish PSD from PSND. Furthermore, the ToA and HtA in the naming animals-task gait paradigm discriminated AD from PSD (moderate accuracy, AUC > 0.700, p ≤ 0.01). Thus, specific gait characteristics could allow early identification of PSD and may allow non-invasive discrimination between PSD and AD, or even other subtypes of dementia.