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The Microbial Prospecting of Oil and Gas (MPOG) is applied to the matured development area in the Satellite Oilfield in Daqing for the first time. And with the MPOG, the hydrocarbon accumulation regulation controlled by faults is interpreted, and the matured development area is extended out to western part. At the same time, four microbial anomalies are discovered. The MPOG results were verified by the hydrocarbon-bearing conditions of the 10 drilled wells within the following half a year, and the consistent rate is up to 80%, which add about 300×104 t of the explored petroleum-in-place. Through study and successful application of MPOG to the Satellite Oilfield, the basis of the application from the exploration to development field is substantially established, and standard system of microbial anomaly is more precisely built for the Satellite Oilfield, which provided an effective explanatory item for the studied and adjacent region, even for the whole Songliao Basin, and also a new technique for complicated reservoirs, especially for the later stage development of litho-reservoirs in China. Therefore, it must have profound influence upon the oil and gas exploration, especially upon the remaining oil, extension and reserves addition of the developed area in China.