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Numerical techniques are widely used in conventional engineering practice for obtaining the stress versus settlement or load versus displacement (P–δ) behavior, respectively, and use the information in the design of shallow and deep foundations, extending the principles of saturated soil mechanics. This Companion Paper II summarizes the details of numerical techniques that can be used in the design of shallow and deep foundations in unsaturated soils. The two key properties required for performing the numerical techniques are the shear strength and the modulus of elasticity of unsaturated soils. A user-friendly subroutine (USDFLD) has been developed for use in the commercial software, ABAQUS to predict the variation of these soil properties with respect to matric suction. The only additional information required for performing the numerical modeling in addition to the conventional soil properties is the soil–water characteristic curve. Good agreements were observed between the proposed numerical techniques and from theoretical approaches and experimental studies. The numerical techniques discussed in this paper are simple and can be used by the geotechnical engineers in the design of shallow and pile foundations in unsaturated soils.

Bearing capacity is one of the key properties required in the design of shallow and deep foundations. In conventional engineering practice, simple approaches are widely used for determining the bearing carrying capacity of foundations based on the shear strength parameters of saturated soils. However, foundations are typically placed in part or fully in the soil zone above the natural ground water table, which is in a state of unsaturated condition. The shear strength of unsaturated soils is significantly influenced by the matric suction. The bearing capacity of foundations cannot be reliably determined by extending conventional soil mechanics principles for soils that are in a state of unsaturated condition. This Companion Paper I, introduces how the shear strength can be used as a tool in the interpretation and prediction of the bearing capacity of foundations in unsaturated soils. In addition, both theoretical and experimental studies related to the bearing capacity of unsaturated soils are succinctly summarized. Numerical techniques that can be used for predicting the stress versus settlement behavior used for the design of shallow and deep foundations are summarized in Companion Paper II. The succinctly summarized information in the companion papers are valuable for geotechnical engineers for understanding and implementing the mechanics of unsaturated soils in the design of shallow and deep foundations.

This paper presented a field study of the reinforced soil wall (RSW) with a geogrid wrap-around facing. In addition to the conventional monitoring content, the strain of the face-wrapping geogrid, which was neglected in most previous studies, was monitored during the construction process. The positional relationship between the maximum vertical earth pressure and horizontally laid geogrid strain was revealed by using the proposed oblique dragging effect. It was found that the strain on the face-wrapping geogrid occurred mainly in the early stage of construction. The oblique dragging effect existing in the flexible RSWs increased the vertical earth pressure and changed its distribution law, resulting in the position of the maximum vertical soil pressure appearing behind the position of the maximum horizontally laid geogrid strain. The horizontal earth pressure at different positions behind the wall was obviously lower than the theoretical result by using Rankine's theory. Also, a modified 0.3H method (where H refers to the wall height) was presented to account for the slope of the wall face, which could be used to determine the potential sliding surfaces for single-stage RSWs and two-tier RSWs with small offset.

The rapid spread of drug-resistant pathogens threatens human health. To address the current antibacterial dilemma, the development of antibiotic-free strategies using nanotechnology is imperative. In this study, silver nanoparticles (Ag-PC NPs) with pH-sensitive charge reversal and self-aggregation capacities are successfully synthesized. In the acidic microenvironment of bacterial biofilms, protonation of the surface peptide enhances the affinity of Ag-PC NPs for bacteria, which can make Ag-PC NPs prone to target and penetrate into biofilms, and the self-aggregated capacity helps Ag-PC NPs remain in biofilms for a long time to disrupt bacterial biofilm formation. In addition, biocompatible Ag-PC NPs are utilized in three types of bacteria-infected animal models. They exhibit an excellent performance in killing bacteria, inhibiting plaque biofilms, and ameliorating inflammatory responses. In conclusion, this study offers new insights into antibiotic-free antibacterial strategies, and exhibits promising application prospects.

Bisphosphonates (BPs) are routinely administered for the treatment of turnover bone diseases. To avoid the undesirable adverse effects of long-term usage of bisphosphonates and improve their bioavailability in the bone microenvironment, we initially encapsulated risedronate (RIS) molecules inside nanoscale zeolitic imidazolate framework-8 particles (nZIF-8) by a one-step synthesis method to generate RIS@ZIF-8 nanoparticles. RIS@ZIF-8 nanoparticles displayed high loading encapsulation efficiency (64.21 ± 2.48%), good biocompatibility, controlled drug release capacity, and dual effects for bone regeneration. This work explored the potential of RIS@ZIF-8 nanoparticles, which could not only enhance ATP production, induce extracellular matrix (ECM) mineralization, and upregulate the expression levels of osteogenic genes but also effectively inhibit the formation of multinucleated giant osteocasts and decrease the Rankl/Opg ratio. Overall, RIS@ZIF-8 nanoparticles could be a very promising approach to synergistically enhance osteogenic and antiresorptive properties for bone regeneration, which could be utilized for the local treatment of bone defects.

Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field. Our previous studies have demonstrated that nano ZIF-8-based titanium implants could promote osseointegration; however, its osteogenic capacity and the related mechanisms in bone regeneration have not been fully clarified. Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+. Moreover, the cellular uptake mechanisms of the nanoparticles were thoroughly clarified. Specifically, nano ZIF-8 could enter the rBMSC cytoplasm probably via caveolae-mediated endocytosis and macropinocytosis. The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis may play a role in inducing activation of key osteogenic pathways. Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs. Taken together, this study helps to elucidate the mechanism by which nano ZIF-8 regulates osteogenesis and suggests it to be a potential biomaterial for constructing multifunctional composites in bone tissue engineering.

This paper proposes an analytical method for estimating the load versus displacement curves for laterally loaded rigid piles in unsaturated soils taking account the influence of matric suction. The distribution of lateral soil resistance along the pile in this method considers three distinct states: (i) pre-tip yield state; (ii) post-tip yield state; and (iii) ultimate limiting state. The lateral earth pressure coefficient, Kc required in this method is derived by extending the three-dimensional (3D) effect factor for drained loading. A numerical technique is also proposed which incorporates a user-defined subroutine (USDFLD) into ABAQUS for simulating the behavior of laterally loaded piles extending 3D finite element analysis taking account of nonlinear behavior of shear strength and modulus of elasticity with respect to matric suction. The proposed analytical method and numerical techniques were validated, respectively using the results of seven field studies and two laboratory tests published from the literature. In addition, close agreement was observed between the predictions of the proposed analytical method and numerical technique for a single pile example problem placed in a soil with varying groundwater table conditions. The summarized studies are promising for use in practice for the rational design of laterally loaded piles in unsaturated soils.

Objective To study the feasibility of measuring root surface area (RSA) by 3D scanning technique and cone beam computed tomography (CBCT) reconstruction in vitro. Design Twenty extracted teeth (10 single-rooted teeth and 10 multi-rooted teeth) were collected in this study. The RSA of the extracted teeth was measured by the membrane technique, 3D scanning technique, and CBCT reconstruction. A standard part was also designed to check the accuracy of each method. All statistical analyses were performed using the SPSS software. Results According to the results of one-way ANOVA, there was no significant difference among the values of RSA measured by the three techniques (p > 0.05). The results of Wilcoxon matched-pairs signed-rank test further demonstrated that there was no significant difference among the values of RSA in both single- and multi-rooted teeth measured by the three techniques (p > 0.05). Conclusions The membrane technique, the 3D scanning technique, and CBCT reconstruction are novel reliable techniques for measuring the RSA in both single- and multi-rooted teeth, which will provide wide clinical applications in the future.

Due to their high specific surface area, graphene oxide and graphene oxide-base nanoparticles have great potential both in dual-drug delivery and combination chemotherapy. Herein, we developed cisplatin (Pt) and doxorubicin (DOX) dual-drug-loaded PEGylated nano-graphene oxide (pGO) to facilitate combined chemotherapy in one system. In this study, nano-sized pGO-Pt/DOX ranged around 161.50 nm was fabricated and characterized using zeta-potential, AFM, TEM, Raman, UV-Vis, and FTIR analyses. The drug delivery efficacy of Pt was enhanced through the introduction of pGO, and the final weight ratio of DOX: Pt: pGO was optimized to 0.376: 0.376: 1. In vitro studies revealed that pGO-Pt/DOX nanoparticles could be effectively delivered into tumor cells, in which they induced prominent cell apoptosis and necrosis and exhibited higher growth inhibition than the single drug delivery system or free drugs. The pGO-Pt/DOX induced the most prominent cancer cell apoptosis and necrosis rate with 18.6%, which was observed almost 2 times higher than that of pGO-Pt or pGO-DOX groups. in the apoptosis and necrotic quadrants In vivo data confirmed that the pGO-Pt/DOX dual-drug delivery system attenuated the toxicity of Pt and DOX to normal organs compared to free drugs. The tumor inhibition data, histopathology observations, and immunohistochemical staining confirmed that the dual-drug delivery system presented a better anticancer effect than free drugs. These results clearly indicated that the pGO-Pt/DOX dual-drug delivery system provided the means for combination drug delivery in cancer treatment.

Wound treatment is a long-lasting clinical issue. Poor angiogenesis leading to delayed wound closure causes huge challenges for healing. Functional electrospun membranes have been established as an efficient strategy to promote wound recovery by protecting and improving vascular regeneration. Here, we aimed to investigate the effect of tazarotene, an active drug for angiogenesis, loaded in aligned electrospun nanofibrous barrier on a soft tissue wound. This aligned membrane was arranged in a single direction, and tazarotene could be released from its nanofibers sustainably. The in vitro study demonstrated that compared with the random drug-loaded or other control groups, the aligned tazarotene-loaded membranes [poly-caprolactone (PCL)/AT] could stimulate proliferation, migration, angiogenesis, and vascular endothelial growth factor secretion and its gene expression of human umbilical vein endothelial cells. Furthermore, the in vivo model showed that the prepared tazarotene-loaded aligned membrane significantly accelerated the speed of healing, improved the neovascularization and re-epithelialization, and inhibited the inflammatory reaction in the wound area. All these results above indicated that the PCL/AT nanofibrous dressing, which could promote angiogenesis because of both stimulation of structure and chemical signals, is a promising wound-caring material.

We have witnessed abundant breakthroughs in research on the bio-applications of graphene family materials in current years. Owing to their nanoscale size, large specific surface area, photoluminescence properties, and antibacterial activity, graphene family materials possess huge potential for bone tissue engineering, drug/gene delivery, and biological sensing/imaging applications. In this review, we retrospect recent progress and achievements in graphene research, as well as critically analyze and discuss the bio-safety and feasibility of various biomedical applications of graphene family materials for bone tissue regeneration.