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To improve the hydration and functional properties of dietary fiber from burdock root by ultrasonic treatment combined with hydrogen peroxide modification. The dietary fiber from burdock root was modified by ultrasonic, alkaline hydrogen peroxide, ultrasound-alkaline hydrogen peroxide and alkaline hydrogen peroxide-ultrasonic methods, which was aim to explore the effects of different methods of modification on the structure and functional properties. The results showed that the expansibility and water-holding capacity of dietary fiber from burdock root were significantly (P

The continuous emergence of highly immune-evasive SARS-CoV-2 variants has challenged vaccine efficacy. A vaccine that can provide broad protection is desirable. We evaluated the immunogenicity of a series of monovalent and bivalent adenovirus-vectored vaccines containing the spikes of Wildtype (WT), Beta, Delta, Omicron subvariants BA.1, BA.2, BA.2.12.1, BA.2.13, BA.3, BA.5, BQ.1.1, and XBB. Vaccination in mice using monovalent vaccines elicited the highest neutralizing titers against each self-matched strain, but against other variants were reduced 2− to 73-fold. A bivalent vaccine consisting of WT and BA.5 broadened the neutralizing breadth against pre-Omicron and Omicron subvariants except XBB. Among bivalent vaccines based on the strains before the emergence of XBB, a bivalent vaccine consisting of BA.2 and BA.5 elicited the most potent neutralizing antibodies against Omicron subvariants, including XBB. In mice primed with injected WT vaccine, intranasal booster with a bivalent vaccine containing XBB and BA.5 could elicit broad serum and respiratory mucosal neutralizing antibodies against all late Omicron subvariants, including XBB. In mice that had been sequentially vaccinated with WT and BA.5, intranasal booster with a monovalent XBB vaccine elicited greater serum and mucosal XBB neutralizing antibodies than bivalent vaccines containing XBB. Both monovalent and bivalent XBB vaccines induced neutralizing antibodies against EG.5. Unlike the antibody response, which is highly variant-specific, mice receiving either monovalent or bivalent vaccines elicited comparable T-cell responses against all variants. Furthermore, intranasal but not intramuscular booster induced antigen-specific lung resident T cells. This study provides insights into the design of the COVID-19 vaccine and vaccination strategies.

ObjectiveTo explore the effectiveness of Tai Chi on cognitive function in patients with mild cognitive impairment (MCI).MethodsAccording to the PRISMA guidelines, randomized controlled trial (RCT) literature on the efficacy of Tai Chi on MCI patients was searched in China National Knowledge Network (CNKI), China Biomedical Literature Database (CBM), Wanfang Data, China Scientific Journal Database (VIP), PubMed, Embase, Duxiu Database, Web of Science and Cochrane Library from their inception to April 2024. The risk of bias in each study was appraised using the Cochrane risk−of−bias tool using Revman 5.4. Random effect model or fixed effect model was used to compare the effects of Tai Chi and control conditions on baseline and post−intervention assessment of cognitive function. Meta−analysis was performed using Stata15.0 software.ResultsNine studies fulfilled the inclusion criteria. Tai Chi significantly improved Montreal Cognitive Assessment (MoCA, SMD, 1.43, p

Backgrounds: Existing studies on the treatment of emergency infectious diseases have primarily focused on the pathogen and the human immune system. However, human health is intricately connected to environmental factors, and this interaction becomes particularly during large-scale public health emergencies. Few studies have examined the impact of spatial differences in the microenvironment on the rehabilitation rate of patients with the Omicron variant infection. Objective: This study employs causal inference statistical methods and spatial analysis to investigate how the hospital microenvironment affects the rehabilitation of COVID-19 patients in a mobile cabin hospital, so as to provide a scientific basis for the spatial arrangement of patients in mobile cabin hospitals. Design: Observational study. Methods: This study used the clinical information of 6291 patients admitted in a mobile cabin hospital in Shanghai, from April 9 to May 9, 2022, during the pandemic. Exploratory spatial data analysis and fixed-effects regression analysis were conducted to understand whether the microenvironment around the patients’ beds in the cabin impacted their rehabilitation. Results: The results indicate that the rehabilitation condition of patients is affected by spatial differences of microenvironment. Both the mean and minimum CT values of the surrounding patients show a significant positive correlation with the rehabilitation of patients. The further the distance from the vent is, the more the rehabilitation speed of the patients is affected by the CT values of the surrounding patients. Conclusion: These findings offer valuable insights for bed allocation, patients’ stratification and management, and ventilation management in the mobile cabin hospitals during public health emergencies.

Abstract Tuning immune‐cold tumor hot has largely attracted attention to improve cancer treatment, including immunotherapy and antibody‐drug conjugates (ADCs). Utilizing multiomic analyses and experimental validation, this work identifies a pivotal role for the USP10/B7‐H4 proteolytic axis in mediating the interplay between tumor immune responses and ADC efficacy, particularly for sacituzumab govitecan (SG) in treating triple negative breast cancers (TNBCs). Mechanistically, the inhibition of autocrine motility factor receptor (AMFR)‐mediated ubiquitylation of B7‐H4 by the deubiquitinase USP10 leads to the stabilization of B7‐H4, which suppresses tumor immune activity and reduces SG treatment effectiveness. Pharmacological inhibition of USP10 promotes the degradation of B7‐H4, enhancing tumor immunogenicity and consequently improving the tumor‐killing efficacy of SG. In preclinical TNBC models, suppression of USP10/B7‐H4 proteolytic axis is effective in increasing SG killing efficacy and reducing tumor growth, especially for the tumors with the USP10high/B7‐H7high signature. Collectively, these findings uncover a novel strategy for targeting the immunosuppressive molecule B7‐H4 for cancer therapy.

Sick sinus syndrome (SSS) is a grave medical condition that can precipitate sudden death. The pathogenesis of SSS remains incompletely understood. Existing research postulates that the fundamental mechanism involves increased fibrosis of the sinoatrial node and its surrounding tissues, as well as disturbances in the coupled-clock system, comprising the membrane clock and the Ca2+ clock. Mitochondrial dysfunction exacerbates regional tissue fibrosis and disrupts the functioning of both the membrane and calcium clocks. This plays a crucial role in the underlying pathophysiology of SSS, including mitochondrial energy metabolism disorders, mitochondrial oxidative stress damage, calcium overload, and mitochondrial quality control disorders. Elucidating the mitochondrial mechanisms involved in the pathophysiology of SSS and further investigating the disease's mechanisms is of great significance.

BackgroundDeep Brain Stimulation (DBS) has been widely applied and accepted in the treatment of neurological and psychiatric disorders. Despite numerous studies exploring the effects of DBS on the progression of neurodegenerative diseases and the treatment of advanced Parkinson’s disease (PD), there is a limited number of articles summarizing this research. The purpose of this study is to investigate the current trends, hot topics, and potential in research surrounding DBS therapy for PD, as well as to anticipate the challenges of such research.MethodsWe searched the Web of Science Core Collection database (WoSCC) for DBS research literature related to PD published from January 2014 to January 2024, utilized CiteSpace, VOS viewer, the bibliometric online analysis platform, Scimago Graphica, Microsoft Excel 2021, and R software version 4.2.3 for data analysis. And we conducted quantitative research on publications, citations, journals, authors, countries, institutions, keywords, and references, visualized the results in network graphs.ResultsFrom 2014 to 2024, papers from 39 journals from 11 countries were among the top 100 cited. Most papers were published in Neurology, with the highest average citations per paper in Nature Neuroscience. The United States (US) contributed the most publications, followed by the United Kingdom (UK) and Germany. In terms of total publications, University College London (UCL) contributed the most papers. The primary classifications of articles were Clinical Neurology, Neurosciences, and Surgery. The top five keywords were subthalamic nucleus, DBS, PD, medical therapy, and basal ganglia. Cluster analysis indicates that DBS research focus on improving quality of life and applying computational models.ConclusionThrough bibliometric analysis, researchers could quickly and clearly understand the hotspots and boundaries of their research field, thus guiding their research direction and scope to improve research efficiency and the quality of outcomes. Although studies indicate that DBS is currently a crucial method for treating advanced PD, in the long run, creating a personalized, low-cost treatment regimen with precise targeting and long-term efficacy poses a challenge.

Air pollution, particularly fine particulate matter and gaseous pollutants including NO2 and NOx, presents significant public health challenges. While the harmful effects of these pollutants are well-documented, the molecular mechanisms underlying their impact on health remain incompletely understood. In this study, we utilized genome-wide association study (GWAS) data from the UK Biobank, expression quantitative trait loci (eQTL) data from the Genotype-Tissue Expression (GTEx) project, and protein quantitative trait loci (pQTL) data from the Atherosclerosis Risk in Communities (ARIC) study to conduct comprehensive analyses using the Unified Test for Molecular Signatures (UTMOST), Transcriptome-wide Association Studies (TWAS), and Proteome-wide Association Studies (PWAS). To integrate and synthesize these analyses, we developed the AirSigOmniTWP Hub, a specialized platform designed to consolidate and interpret the results from UTMOST, TWAS, and PWAS. TWAS analysis identified a significant association between PM10 exposure and the gene INO80E in females (P = 4.37×10⁻⁵, FDR = 0.0383), suggesting a potential role in chromatin remodeling. PWAS analysis revealed a significant association between NOx exposure and the gene PIP in females (P = 2.28×10⁻⁵, FDR = 0.0299), implicating its involvement in inflammatory pathways. Additionally, UTMOST analyses uncovered significant associations between various pollutants and genes including NCOA4P3 and SPATS2L with PM2.5 exposure, indicating potential mechanisms related to transcriptional regulation and gene-environment interactions.

Background: Nasopharyngeal carcinoma (NPC) is a highly diverse and aggressive cancer type, leading to varying prognoses and responses to immunotherapy. This study aims to develop a protein-based signature that provides new insights into assessing the prognosis and immunotherapeutic response in NPC patients. Methods and Results: We obtained transcriptomic and proteomic data for NPC from TCGA and CPTAC databases, respectively. Differentially expressed proteins with prognostic significance were identified using limma combined with uniCox analysis. A prognostic protein signature was created utilizing the LASSO algorithm. Receiver operating characteristic (ROC) curve analysis along with Kaplan-Meier survival analysis was conducted to assess the predictive accuracy of this signature. To evaluate immune infiltration levels among patients categorized by high or low risk scores (RPscores), we employed ssGSEA and ESTIMATE methods, while TIDE was used to forecast responses to immunotherapy. Our research pinpointed four critical prognostic proteins: CdSTA, AGR3, DUSP14, and LRRC17, allowing us to compute risk scores (RPscores). Kaplan-Meier curves demonstrated that individuals in the low-risk category exhibited better survival rates. Furthermore, RPscore effectively predicted overall survival across both training and testing cohorts. The ssGSEA results indicated that RPscore is linked with an immune-suppressive microenvironment correlating with diminished immune responses. Notably, DUSP14 showed significant upregulation in NPC cases; its role in promoting cell invasion and metastasis was confirmed through in vitro studies. Conclusion: We have established a robust protein-related signature capable of accurately forecasting prognosis as well as immunotherapy outcomes for NPC patients. Moreover, DUSP14 emerged as a potential therapeutic target due to its strong association with patient prognosis in nasopharyngeal carcinoma.

Abstract The Rho GTPase activating protein family (ARHGAPs) is expressed in pancreatic adenocarcinoma (PAAD) but its function is unclear. The aim of this study was to explore the role and potential clinical value of ARHGAPs in PAAD. Using TCGA and GEO databases to analyze expression of ARHGAPs in PAAD and normal tissues. Survival curve was drawn by Kaplan–Meier. ARHGAPs were integrated analyzed by GEPIA2, TIMER, UCLCAN, cBioPortal and R language. Protein level and prognostic value were evaluated via IHC staining or survival analysis. We totally identify 18 differentially expressed (DE) ARHGAPs in PAAD. Among the 18 DE genes, 8 were positively correlated with tumor grade; abnorrmal expression of 5 was positively correlated with copy number variation; expression of 4 was positively correlated with promoter hypomethylation. Multivariate Cox regression identified ARHGAP5, ARHGAP11A, and ARHGAP12 as independent prognostic factors of PAAD. The function of ARHGAPs was mainly related to GTPase activity and signaling, axon guidance, proteoglycans in cancer and focal adhesion. Expression of 7 ARHGAPs was strongly correlated with immune infiltration. Immunohistochemistry showed increased protein levels of ARHGAP5, ARHGAP11A, and ARHGAP12 in PAAD tissues. Survival analysis confirmed a negative correlation between ARHGAP5, ARHGAP11A, and ARHGAP12 expression and patient prognosis. Multivariate Cox regression proved ARHGAP5, ARHGAP11A, and ARHGAP12 could serve as independent prognostic indicators for PAAD. Finally, this study verified ARHGAP5, ARHGAP11A, and ARHGAP12 as independent prognostic factors in PAAD, suggesting their significance for the diagnosis and treatment of PAAD.

Abstract Background The link between diabetes and dementia risk is not well understood. This study evaluates the factors linking diabetes to dementia onset, providing guidance for preventing dementia in diabetic patients. Methods This analysis utilized databases such as PubMed, Embase, Web of Science, and the Cochrane Library to review literature from January 31, 2012, to March 5, 2023. Articles were rigorously assessed using specific inclusion and exclusion criteria. The Newcastle-Ottawa Scale (NOS) was used to evaluate the quality of the studies. Data analysis was performed with STATA 15.0. Results The study analyzed 15 articles, covering 10,103,868 patients, with 8,821,516 diagnosed with diabetes. The meta-analysis reveals a substantial association between diabetes and an increased risk of dementia [RR: 1.59, 95%CI (1.40–1.80), P

Highlights An innovative class of versatile form-stable composite phase change materials (CPCMs) was fruitfully exploited, featuring MXene/phytic acid hybrid depositing on non-carbonized wood as a robust support. The wood-based CPCMs showcase enhanced thermal conductivity of 0.82 W m−1 K−1 (4.6 times than polyethylene glycol) as well as high latent heat of 135.5 kJ kg−1 (91.5% encapsulation) with thermal durability and stability throughout at least 200 heating and cooling cycles. The wood-based CPCMs have good solar-thermal-electricity conversion, flame-retardant, and electromagnetic shielding properties.

SNF1-related protein kinase 2 (SnRK2) family members are essential components of the plant abscisic acid (ABA) signaling pathway initiated by osmotic stress and triggering a drought stress response. This study characterized the molecular properties of TaSnRK2.4 and its function in mediating adaptation to drought in Triticum aestivum. Transcripts of TaSnRK2.4 were upregulated upon drought and ABA signaling and associated with drought- and ABA-responsive cis-elements ABRE and DRE, and MYB and MYC binding sites in the promoter as indicated by reporter GUS protein staining and activity driven by truncations of the promoter. Yeast two-hybrid, BiFC, and Co-IP assays indicated that TaSnRK2.4 protein interacts with TaPP2C01 and an ABF transcription factor (TF) TaABF2. The results suggested that TaSnRK2.4 forms a functional TaPP2C01-TaSnRK2.4-TaABF2 module with its upstream and downstream partners. Transgene analysis revealed that TaSnRK2.4 and TaABF2 positively regulate drought tolerance whereas TaPP2C01 acts negatively by modulating stomatal movement, osmotic adjustment, reactive oxygen species (ROS) homeostasis, and root morphology. Expression analysis, yeast one-hybrid, and transcriptional activation assays indicated that several osmotic stress-responsive genes, including TaSLAC1-4, TaP5CS3, TaSOD5, TaCAT1, and TaPIN4, are regulated by TaABF2. Transgene analysis verified their functions in positively regulating stomatal movement (TaSLAC1-4), proline accumulation (TaP5CS3), SOD activity (TaSOD5), CAT activity (TaCAT1), and root morphology (TaPIN4). There were high correlations between plant biomass and yield with module transcripts in a wheat variety panel cultivated under drought conditions in the field. Our findings provide insights into understanding plant drought response underlying the SnRK2 signaling pathway in common wheat.

Objectives To explore the effects of physical activity on anxiety levels in college students, as well as to examine the mediating role of emotion regulation. Methods A convenience sample of 1,721 college students from Shanghai, Jiangsu, Shandong, Guangxi, and Hunan was used to conduct an evaluation and a survey through the Physical Activity Rating Scale (PARS-3), the Anxiety Self-Rating Scale (SAS), and the Emotion Regulation Scale (ERQ). Results College students’ anxiety level, cognitive reappraisal, and expression inhibition scores were (44.72 ± 10.37), (30.16 ± 6.51), and (16.96 ± 4.99), respectively. There were significant grade and physical activity level differences in anxiety levels and cognitive reappraisal, and significant gender and physical activity level differences in expression inhibition among college students. Process model 4 mediated effect regression analysis showed that physical activity had a significant positive effect on cognitive reappraisal (R2 = 0.14, β = 0.04, P

Water resource asset (WRA) accounting holds great importance in ecological civilization construction. Existing WRA accounting methods heavily rely on statistical data, resulting in issues such as missing and inaccessible data. Moreover, they only consider the value brought by the physical resources, such as water quantity and quality, while neglecting the value brought by the ecological functions. Therefore, by fully exploiting the rapid, objective, and efficient advantages of remote sensing (RS) in monitoring surface objects, this article develops a surface WRA (SWRA) accounting method based on multi-source RS data. First, a representation model is innovatively proposed, with full consideration of the ecological service functions offered by water resources. Specifically, the SWRAs are represented by two parts: tangible and intangible assets. The tangible asset refers to the quantifiable stock of water resources. Surface water volume is adopted as the indicator for tangible assets in this article. The intangible asset, which primarily embodies the ecological service functions provided by water resources, encompasses five major categories: flood regulation, carbon fixation, oxygen release, water purification, and water conservation. Furthermore, due to different units, the total amounts cannot be summed or compared directly. Therefore, this article utilizes price tools to convert SWRAs into price value, ultimately achieving SWRA accounting. The established method was tested in Miyun, Beijing, China, from 2013 to 2023. The findings demonstrate that the SWRA value reached its peak in 2023, amounting to 56,9368.6×104 yuan, while it had its lowest point in 2014, standing at 14,7402.7×104 yuan. The experimental results indicate that the proposed method can quickly provide the SWRA values for many years, offering a methodological foundation for SWRA asset auditing and enhancing the timeliness of the auditing work.

Abstract Crustal deformation and hydrothermal percolation related to the India‐Asia collision have caused extensive remagnetization of the Tethyan Himalaya Terrane (THT). The present work identified three phases of regional remagnetization during 62.3–50.0 Ma for the east‐central THT. Consequently, a model of three‐stage India‐Asia collision was proposed. The east‐central THT first collided with the southward migrated southern margin of the Lhasa Terrane (LT) at 5.4 ± 0.9°N during 62.3–60.9 Ma. Subsequently, the THT continuously moved northward and pushed the southern margin of the LT back to its original position prior to the initiation of fore‐arc and back‐arc extension on both sides of the Gangdese magmatic arc. Since the final suturing of the THT with Asia at ∼10°N during 59.8–58.0 Ma, the east‐central THT remained stationary until India collided with it at 10.9 ± 5.1°N at ∼50.0 Ma.

Abstract Background Paradoxical embolism is a rare cause of acute arterial occlusion. This phenomenon arises when embolic material travels from the venous system crosses an abnormal shunt such as patent foramen ovale, atrial septal defects, ventricular septal defects, or pulmonary arteriovenous malformations, into the arterial system. Impending paradoxical embolism refers to the presence of an entrapped thrombus in the patent foramen ovale. Case presentation We report a case of a 68-year-old female patient who presented with an impending paradoxical embolism, alongside both concomitant pulmonary embolism and myocardial infarction with ST-segment elevation. Swiftly addressed through emergency cardiac surgery and systemic anticoagulation, the patient’s condition was effectively treated. Conclusions While the ideal treatment strategy for impending paradoxical embolism remains a topic of debate due to limited and inconclusive evidence, emergent open surgery should be contemplated in patients as it signifies a critical clinical emergency.

Niobium telluride (NbTe2), a kind of few-layer two-dimensional (2D) transition metal dichalcogenides (TMDs) material, has been theoretically predicted with nonlinear absorption properties and excellent optical response. Herein, we experimentally demonstrated an Er-doped fiber (EDF) laser based NbTe2 as saturable absorber (SA). Few-layer NbTe2 nanosheets were successfully prepared by adopting the commonly used liquid-phase exfoliation (LPE) method. The nonlinear optical response of highly stable few-layer NbTe2 was investigated through an open-aperture Z-scan laser measurement, the nonlinear absorption coefficient was 2.45 × 10−11 m/W. Both Q-switched and mode-locked operation centered at 1 559 nm were recorded based on NbTe2 SA. The pulse duration was varied from 4.88 μs to 1.75 μs, and the adjustable range of repetition frequency is changed from 44.01 kHz to 64.12 kHz in passively Q-switched operation. Furthermore, a constant repetition rate of 5.33 MHz and pulse width of 2.67 ps were observed in mode-locked operation. Our experimental results fully reveal the nonlinear optical properties of NbTe2 used in pulsed fiber lasers and broaden its ultrafast applications in the optics field.

Fiberglass-reinforced ultraviolet cured-in-place pipe (UV–CIPP) composite material is one of the most trenchless materials for underground pipelines’ rehabilitation. In this paper, the bending resistance and damage evolution mechanism of glass fiber-reinforced UV-CIPP composites were investigated under the influence of glass fiber structure, the number of layers of glass fibers, the angle of fiber layups, and the thickness of the material by high-definition video, SEM and infrared thermal imaging. The results indicate that the damage evolution modes of UV-CIPP materials mainly include: (1) fiber pull-out and overall fiber bundle tensile failure caused by strong interfacial bonding, (2) fiber/matrix debonding and delamination, fiber fracture, and matrix cracking caused by weak interfacial bonding. Furthermore, among the seven different fiberglass structures of UV-CIPP materials, the [0°/90°] warp-knit axial/short-cut felt fiberglass fabric exhibit the best flexural performance, with a bending strength of 412 MPa and a bending modulus of 16.1 GPa for the 4 layers glass fiber fabric. Moreover, in the bending process of UV-CIPP materials, the surface temperature rises primarily due to fiber break, the temperature transition aligning with the stress transition. Finally, the energy release is mainly caused by the failure of the glass fibers, but the resin contributed comparatively little. This study provides a scientific reference for the structural design and optimization of UV-CIPP materials.

Abstract Gastric cancer (GC) represents a significant burden of cancer-related mortality worldwide, underscoring an urgent need for the development of early detection strategies and precise postoperative interventions. However, the identification of non-invasive biomarkers for early diagnosis and patient risk stratification remains underexplored. Here, we conduct a targeted metabolomics analysis of 702 plasma samples from multi-center participants to elucidate the GC metabolic reprogramming. Our machine learning analysis reveals a 10-metabolite GC diagnostic model, which is validated in an external test set with a sensitivity of 0.905, outperforming conventional methods leveraging cancer protein markers (sensitivity

The most essential cause of the fracture of the dropper is the effect of alternating stress for a long time. Therefore, in order to ensure the safe operation of high-speed railways, the influence of moving loads on the stress of a dropper was investigated in this study. Due to a high-voltage catenary system, it is very difficult to measure the moving load. Thus, the uplift displacement measured by some software and hardware devices has been applied to the contact wire instead of the moving load. The response equation for the contact wire has been derived so as to determine the initial and boundary conditions of each dropper. Then it was combined with the equation for vibration analysis of the dropper and the stress of each dropper was calculated by using the finite-difference method based on a written MATLAB program. The results show that the dropper stress, during a certain period goes through two stages of immediate rebound and bending compression when the uplift displacement is large. After the pantograph passes, the vibration of the dropper tends to be smooth; also, dropper stress variation with timecan be described by three stages: immediate rebound, vibration attenuation, and bending compression. In addition, the maximum tensile stress of dropper Ⅳ was the highest. It indicates that dropper Ⅳ was more prone to fracture than other droppers.

Persistent HPV infection is a major risk factor for the subsequent development of cervical cancer. LAMP is simple and suitable for field detection in the resource-limited settings. In this study, hydroxy naphthol blue (HNB)-based visual LAMP and evagreen-based fluorescent LAMP coupled with a microfluidic chip (LAMP-chip) were established for the field detection of seven subtypes of HPV. The analytical sensitivity was 19–233 copies/reaction. The overall clinical sensitivity was 97.35% for visual LAMP and 98.23% for LAMP-chip. Both LAMP assays exhibited 100% specificity and were completed in less than 50 min. Additionally, both assays did not require complicated nucleic acid extraction and purification steps. A complete quality control monitoring system (including internal control, positive quality control and negative control) in the LAMP assays further ensured the credibility of the results. Our findings demonstrated that the proposed LAMP assays have the potential to be applied in the testing of common HPV DNA in field investigations (visual LAMP) or within communities and primary health centers (LAMP-chip).

BackgroundAccording to data from the World Health Organization (WHO), there is a significant public health issue regarding the increasing number of individuals affected by obesity and overweight on an annual basis. Therefore, it is imperative to urgently identify interventions that can effectively control and improve this condition. Baduanjin, as a medium-intensity exercise, appears a suitable approach for weight reduction among individuals with obesity. This paper aimed to provide a systematic review and meta-analysis of the efficacy of Baduanjin in addressing obesity and overweight, with the ultimate goal of assisting individuals with obesity in finding an effective, safe, and engaging method for weight reduction.MethodsWe conducted a comprehensive search of multiple databases including PubMed, Cochrane Library, Web of Science, Embase, The China National Knowledge Infrastructure (CNKI), The Chinese Scientific Journal Database (VIP), The Chinese Biomedical Literature Database (CBM), and WanFang Database to identify relevant articles published from the inception of each database until September 2023. Specifically, we focused on randomized controlled trials (RCTs) investigating the effects of Baduanjin on weight reduction. Data from these studies were extracted and analyzed using appropriate statistical methods. In cases where there was no significant heterogeneity (I2 < 50%, p > 0.1), we employed a fixed effects model for data synthesis; otherwise, a random effects model was selected. Funnel plots were used to assess publication bias, and the mean difference (MD) was reported as an indicator of treatment group differences.ResultsA total of 420 participants were included in 10 studies. The MD results of the experimental group when compared with the control group were −3.69 (95%CI = −4.97 to −2.40, p < 0.001) for body weight (BW), −5.42 (95%CI = −6.56 to −4.28, p < 0.001) for body mass index (BMI), −1.36 (95%CI = −1.76 to −0.96, p < 0.001) for waist circumference (WC), −3.40 (95%CI = −4.43 to −2.37, p < 0.001) for hip circumference (HC), and −0.03 (95%CI = −0.04 to −0.02, p > 0.1) for the waist-to-hip ratio (WHR). All of the values in the experimental group showed significant difference. The results of the Egger’s test (t = 1.43, p = 0.190) suggest that there was no substantial bias present within the data analysis process. The safety profile revealed no adverse events reported across all 10 studies.ConclusionBaduanjin could be effective in reducing weight, and the practice of Baduanjin has the potential to regulate BW, BMI, WC, HC, and WHR. However, further well-designed RCTs are still necessary to provide more robust evidence in the future.Systematic review registrationhttp://www.crd.york.ac.uk/PROSPERO/, identifier CRD42024513789.

Sleep–wake (SW) cycle detection is a key step for extracting temporal sleep metrics from actigraphy. Various supervised learning algorithms have been developed, yet their generalizability from sensor to sensor or study to study is questionable. In this paper, we detail and validate an unsupervised algorithm—CircaCP—for detecting SW cycles from actigraphy. It first uses a robust cosinor model to estimate circadian rhythm, then searches for a single change point (CP) within each circadian cycle. Using CircaCP, we estimated sleep/wake onset times (S/WOTs) from 2125 individuals’ data in the MESA sleep study and compared the estimated S/WOTs against self-reported S/WOT event markers, using Bland–Altman analysis as well as variance component analysis. On average, SOTs estimated by CircaCP were 3.6 min behind those reported by event markers, and WOTs by CircaCP were less than 1 min behind those reported by markers. These differences accounted for less than 0.2% variability in S/WOTs, considering other sources of between-subject variations. Rooted in first principles of human circadian rhythms, our algorithm transferred seamlessly from children’s hip-worn ActiGraph data to ageing adults’ wrist-worn Actiwatch data. The generalizability of our algorithm suggests that it can be widely applied to actigraphy collected by other sensors and studies.

AimThis research sought to identify the association between sports participation and resilience in children and adolescents as a means to enhance mental health.MethodsA comprehensive survey was carried out, encompassing primary, middle, and high school students from chosen educational institutions. The analytical sample comprised 67,281 students of school age. Sports participation and resilience were evaluated using validated assessment tools, while relevant covariates, such as sex and school grade, were assessed through self-reported questionnaires. Generalized Linear Models were applied to ascertain the association between sports participation and resilience for the entire sample, and separately for subgroups divided by gender or school grade, after controlling for covariates.ResultsAmong the 67,281 school students, males constituted 51.9% of the sample. Approximately 47.1% of the entire sample reported no sports participation, and the average resilience score was 24.7. The regression model analysis revealed that, in the entire sample, increased in sports participation was linked to higher resilience scores (odds ratio [OR] for 1–3 times per month: 1.20, 95%CI: 1.16–1.24; OR for 1–2 times per week: 1.38, 95%CI: 1.33–1.43; OR for 3 times or more per week: 1.72, 95%CI: 1.65–1.79). Analyses stratified by gender and school grade indicated that sports participation was consistently associated with greater resilience.ConclusionThis study provides cross-sectional evidence supporting the positive association between sports participation and the resilience of children and adolescents, underscoring the potential of encouraging sports participation as a strategy for promoting mental health resilience. The findings presented herein should be subject to further confirmation or refutation in future research endeavors.

BackgroundGastric signet ring cell carcinoma (GSRCC) is a rare and highly malignant disease with a poor prognosis. To assess the overall survival (OS) and cancer-specific survival (CSS) of patients with GSRCC, prognostic nomograms were developed and validated using common clinical factors.MethodsThis retrospective cohort study included patients diagnosed with GSRCC between 2011 and 2018 from the National Cancer Center (n = 1453) and SEER databases (n = 2745). Prognostic nomograms were established by identifying independent prognostic factors using univariate and multivariate Cox regression analyses. The calibration curve and C-index were used to assess the predictions. The clinical usefulness of the survival prediction model was further evaluated using the DCA and ROC curves. The models were internally validated in the training cohort and externally validated in the validation cohort. Two web servers were created to make the nomogram easier to use.ResultsPatients with GSRCC were divided into training (n = 2938) and validation (n = 1260) cohorts. The nomograms incorporated six predictors: age, race, tumor site, tumor size, N stage, T stage, and AJCC stage. Excellent agreement was observed between the internal and exterior calibration plots for the GSRCC survival estimates. The C-index and area under the ROC curve were roughly greater than 0.7. Both nomograms had adequate clinical efficacy, as demonstrated by the DCA plots. Furthermore, we developed a dynamic web application utilizing the constructed nomograms available at https://jiangyujuan.shinyapps.io/OS-nomogram/ and https://jiangyujuan.shinyapps.io/DynNomapp-DFS/.ConclusionWe developed web-based dynamic nomograms utilizing six independent prognostic variables that assist physicians in estimating the OS and CSS of patients with GSRCC.

Linear IgA bullous dermatosis (LABD) is a rare autoimmune subepidermal blistering skin disease, manifested by erythematous plaques covered by circular blisters in a pattern of "string of pearls". Immune dysregulation and genetic factors are implicated in the onset of LABD. The pathogenesis includes abnormal deposition of IgA antibodies in the basement membrane zone and abnormal immune response. Although the pathological characteristics are atypical, direct immunofluorescence shows deposition of linear IgA antibody at the basement membrane zone. Advances in molecular biology and immunology offer new possibilities for enhancing precise diagnosis. Personalized treatment has become pivotal, while topical steroids and antihistamines are suitable for mild cases. Severe cases may require systemic immunosuppressants or even biologics. This review aims to summarize the latest research advances in LABD, including epidemiological features, pathogenesis, diagnostic and therapeutic strategies, and to provide an outlook on future research directions.

Abstract Background Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver condition that affects a quarter of the global adult population. To date, only a few NAFLD risk prediction models have been developed for Chinese older adults aged ≥ 60 years. This study presented the development of a risk prediction model for NAFLD in Chinese individuals aged ≥ 60 years and proposed personalised health interventions based on key risk factors to reduce NAFLD incidence among the population. Methods A cross-sectional survey was carried out among 9,041 community residents in Shanghai. Three NAFLD risk prediction models (I, II, and III) were constructed using multivariate logistic regression analysis based on the least absolute shrinkage and selection operator regression analysis, and random forest model to select individual characteristics, respectively. To determine the optimal model, the three models’ discrimination, calibration, clinical application, and prediction capability were evaluated using the receiver operating characteristic (ROC) curve, calibration plot, decision curve analysis, and net reclassification index (NRI), respectively. To evaluate the optimal model’s effectiveness, the previously published NAFLD risk prediction models (Hepatic steatosis index [HSI] and ZJU index) were evaluated using the following five indicators: accuracy, precision, recall, F1-score, and balanced accuracy. A dynamic nomogram was constructed for the optimal model, and a Bayesian network model for predicting NAFLD risk in older adults was visually displayed using Netica software. Results The area under the ROC curve of Models I, II, and III in the training dataset was 0.810, 0.826, and 0.825, respectively, and that of the testing data was 0.777, 0.797, and 0.790, respectively. No significant difference was found in the accuracy or NRI between the models; therefore, Model III with the fewest variables was determined as the optimal model. Compared with the HSI and ZJU index, Model III had the highest accuracy (0.716), precision (0.808), recall (0.605), F1 score (0.692), and balanced accuracy (0.723). The risk threshold for Model III was 20%–80%. Model III included body mass index, alanine aminotransferase level, triglyceride level, and lymphocyte count. Conclusions A dynamic nomogram and Bayesian network model were developed to identify NAFLD risk in older Chinese adults, providing personalized health management strategies and reducing NAFLD incidence.

Orange spotted grouper (Epinephelus coioides) is an important mariculture fish, and genomic breeding of this grouper species has been hindered due to lack of efficient genotyping tools. Here, we developed a single nucleotide polymorphism (SNP) genotyping technology based on multiplex PCR enrichment capture sequencing, which mainly aims at target area for high-throughput sequencing, and 741 SNPs were designed for genomic selection (GS) of growth and ammonia tolerance traits at the same time. The multiplex PCR enrichment capture sequencing assay showed that the genotyping efficiency was more than 99% in the orange-spotted grouper and the predictive accuracy of body weight and ammonia tolerance traits was 82% and 96%, respectively. More importantly, the average identity of the sequences with these SNPs aligned to the genomes of giant grouper (E. lanceolatus) and brown-marbled grouper (E. fuscoguttatus) were both over 96%. Test data showed that the SNP genotyping efficiency was more than 94% in both giant grouper and brown-marbled grouper. In summary, these results indicated that the development of SNP loci and genotyping approach based on the multiple PCR enrichment capture sequencing are suitable for GS of growth and ammonia tolerance traits in various grouper species, and it would provide technical support for practical grouper breeding.

Sugar transporters play important roles in controlling carbohydrate transport and are responsible for mediating the movement of sugars into cells in numerous organisms. In insects, sugar transporters not only play a role in sugar transport but may also act as receptors for virus entry and the accumulation of plant defense compounds. The brown planthopper, Nilaparvata lugens, inflicts damage on rice plants by feeding on their phloem sap, which is rich in sugars. In the present study, we identified 34 sugar transporters in N. lugens, which were classified into three subfamilies based on phylogenetic analysis. The motif numbers varied from seven to eleven, and motifs 2, 3, and 4 were identified in the functional domains of all 34 NlST proteins. Chromosome 1 was found to possess the highest number of NlST genes, harboring 15. The gut, salivary glands, fat body, and ovary were the different tissues enriched with NlST gene expression. The expression levels of NlST2, 3, 4, 7, 20, 27, 28, and 31 were higher in the gut than in the other tissues. When expressed in a Saccharomyces cerevisiae hexose transporter deletion mutant (strain EBY.VW4000), only ApST4 (previously characterized) and NlST4, 28, and 31 were found to transport glucose and fructose, resulting in functional rescue of the yeast mutant. These results provide valuable data for further studies on sugar transporters in N. lugens and lay a foundation for finding potential targets to control N. lugens.

Abstract Background The SNP rs671 of Human aldehyde dehydrogenase (ALDH) is G-A transition at 1510th nucleotides, which is an important clinical indicator of alcoholic liver disease, digestive tract cancer and some drug efficiency. The commonly used genotyping assay of this polymorphism is relatively time-consuming and costly. Finding This study develops a rapid and accurate one-step CRISPR/Cas12b assay to distinguish the G1510A polymorphism of human ALDH2 free of DNA amplification. The method we established requires only one step of adding 1 μl genomic DNA sample to premixed system, and waiting for the acquisition of fluorescent signal, taking approximate 30 min. Conclusions This method provides a potential tool for more accurate and reliable nucleic acid detection with a single base difference and supports the relevant disease diagnosis and personalized medicine.

Minimum spanning tree (MST)-based clustering algorithms are widely used to detect clusters with diverse densities and irregular shapes. However, most algorithms require the entire dataset to construct an MST, which leads to significant computational overhead. To alleviate this issue, our proposed algorithm R-MST utilizes representative points instead of all sample points for constructing MST. Additionally, based on the density and nearest neighbor distance, we improved the representative point selection strategy to enhance the uniform distribution of representative points in sparse areas, enabling the algorithm to perform well on datasets with varying densities. Furthermore, traditional methods for eliminating inconsistent edges generally require prior knowledge about the number of clusters, which is not always readily available in practical applications. Therefore, we propose an adaptive method that employs mutual neighbors to identify inconsistent edges and determine the optimal number of clusters automatically. The experimental results indicate that the R-MST algorithm not only improves the efficiency of clustering but also enhances its accuracy.

BackgroundOur study aimed to develop machine learning algorithms capable of predicting red blood cell (RBC) transfusion during valve replacement surgery based on a preoperative dataset of the non-anemic cohort.MethodsA total of 423 patients who underwent valvular replacement surgery from January 2015 to December 2020 were enrolled. A comprehensive database that incorporated demographic characteristics, clinical conditions, and results of preoperative biochemistry tests was used for establishing the models. A range of machine learning algorithms were employed, including decision tree, random forest, extreme gradient boosting (XGBoost), categorical boosting (CatBoost), support vector classifier and logistic regression (LR). Subsequently, the area under the receiver operating characteristic curve (AUC), accuracy, recall, precision, and F1 score were used to determine the predictive capability of the algorithms. Furthermore, we utilized SHapley Additive exPlanation (SHAP) values to explain the optimal prediction model.ResultsThe enrolled patients were randomly divided into training set and testing set according to the 8:2 ratio. There were 16 important features identified by Sequential Backward Selection for model establishment. The top 5 most influential features in the RF importance matrix plot were hematocrit, hemoglobin, ALT, fibrinogen, and ferritin. The optimal prediction model was CatBoost algorithm, exhibiting the highest AUC (0.752, 95% CI: 0.662–0.780), which also got relatively high F1 score (0.695). The CatBoost algorithm also showed superior performance over the LR model with the AUC (0.666, 95% CI: 0.534–0.697). The SHAP summary plot and the SHAP dependence plot were used to visually illustrate the positive or negative effects of the selected features attributed to the CatBoost model.ConclusionsThis study established a series of prediction models to enhance risk assessment of intraoperative RBC transfusion during valve replacement in no-anemic patients. The identified important predictors may provide effective preoperative interventions.

BackgroundMyocardial infarction (MI) caused by severe coronary artery disease has high incidence and mortality rates, making its prevention and treatment a central and challenging aspect of clinical work for cardiovascular practitioners. Recently, researchers have turned their attention to a novel mechanism of cell death caused by Cu2+, cuproptosis.MethodsThis study integrated data from three MI-related bulk datasets downloaded from the Gene Expression Omnibus (GEO) database, and identified 16 differentially expressed genes (DEGs) related to cuproptosis by taking intersection of the 6378 DEGs obtained by differential analysis with 49 cuproptosis-related genes. Four hub genes, Dbt, Dlat, Ube2d1 and Ube2d3, were screened out through random forest analysis and Lasso analysis. In the disease group, Dbt, Dlat, and Ube2d1 showed low expression, while Ube2d3 exhibited high expression.ResultsFocusing on Ube2d3 for subsequent functional studies, we confirmed its high expression in the MI group through qRT-PCR and Western Blot detection after successful construction of a MI mouse model by left anterior descending (LAD) coronary artery ligation, and further clarified the correlation of cuproptosis with MI development by detecting the levels of cuproptosis-related proteins. Moreover, through in vitro experiments, Ube2d3 was confirmed to be highly expressed in oxygen-glucose deprivation (OGD)-treated cardiomyocytes AC16. In order to further clarify the role of Ube2d3, we knocked down Ube2d3 expression in OGD-treated AC16 cells, and confirmed Ube2d3’s promoting role in the hypoxia damage of AC16 cells by inducing cuproptosis, as evidenced by the detection of MTT, TUNEL, LDH release and cuproptosis-related proteins.ConclusionIn summary, our findings indicate that Ube2d3 regulates cuproptosis to affect the progression of MI.

Biomarkers for classifying and grading gliomas have been extensively explored, whereas populations in public databases were mostly Western/European. Based on public databases cannot accurately represent Chinese population. To identify molecular characteristics associated with clinical outcomes of lower-grade glioma (LGG) and glioblastoma (GBM) in the Chinese population, we performed whole-exome sequencing (WES) in 16 LGG and 35 GBM tumor tissues. TP53 (36/51), TERT (31/51), ATRX (16/51), EFGLAM (14/51), and IDH1 (13/51) were the most common genes harboring mutations. IDH1 mutation (c.G395A; p.R132H) was significantly enriched in LGG, whereas PCDHGA10 mutation (c.A265G; p.I89V) in GBM. IDH1-wildtype and PCDHGA10 mutation were significantly related to poor prognosis. IDH1 is an important biomarker in gliomas, whereas PCDHGA10 mutation has not been reported to correlate with gliomas. Different copy number variations (CNVs) and oncogenic signaling pathways were identified between LGG and GBM. Differential genomic landscapes between LGG and GBM were revealed in the Chinese population, and PCDHGA10, for the first time, was identified as the prognostic factor of gliomas. Our results might provide a basis for molecular classification and identification of diagnostic biomarkers and even potential therapeutic targets for gliomas.

This paper introduces an innovative segmentation model that extends the U-Net architecture with a Squeeze and Excitation (SE) mechanism, designed to enhance the detection of moving objects in video streams. By integrating this model into the ViBe motion detection algorithm, we have significantly improved detection accuracy and reduced false positive rates. Our approach leverages adaptive techniques to increase the robustness of the segmentation model in complex scenarios, without requiring extensive manual parameter tuning. Despite the notable improvements, we recognize that further training is necessary to optimize the model for specific applications. The results indicate that our method provides a promising direction for real-time motion detection systems that require high precision and adaptability to varying conditions.

Human T-cell leukemia virus type I (HTLV-I) is an oncogenic virus whose infection can cause diverse diseases, most notably adult T-cell leukemia/lymphoma (ATL or ATLL), an aggressive and fatal malignancy of CD4 T cells. The oncogenic ability of HTLV-I is mostly attributed to the viral transcriptional transactivator Tax. Tax alone is sufficient to induce specific tumors in mice depending on the promotor used to drive Tax expression, thereby being used to understand HTLV-I tumorigenesis and model the tumor types developed in Tax transgenic mice. Tax exerts its oncogenic role predominantly by activating the cellular transcription factor NF-κB. Here, we report that genetic deletion of NF-κB1, the prototypic member of the NF-κB family, promotes adrenal medullary tumors but suppresses neurofibromas in mice with transgenic Tax driven by the HTLV-I Long Terminal Repeat (LTR) promoter. The adrenal tumors are derived from macrophages. Neoplastic macrophages also infiltrate the spleen and lymph nodes, causing splenomegaly and lymphadenopathy in mice. Nevertheless, the findings could be human relevant, because macrophages are important target cells of HTLV-I infection and serve as a virus reservoir in vivo. Moreover, the spleen, lymph nodes and adrenal glands are the most common sites of tumor cell infiltration in HTLV-I-infected patients. These data provide new mechanistic insights into the complex interaction between Tax and NF-κB, therefore improving our understanding of HTLV-I oncogenic pathogenesis. They also expand our knowledge and establish a new animal model of macrophage neoplasms and adrenal tumors.

The excessive use of antibiotics has resulted in the contamination of the environment with antibiotic resistance genes (ARGs), posing a significant threat to public health. Wastewater treatment plants (WWTPs) are known to be reservoirs of ARGs and considered to be hotspots for horizontal gene transfer (HGT) between bacterial communities. However, most studies focused on the distribution and dissemination of ARGs in hospital and urban WWTPs, and little is known about their fate in industrial WWTPs. In this study, collected the 15 wastewater samples containing N,N-dimethylformamide (DMF) from five stages of the anaerobic anoxic aerobic (AAO) process in an industrial WWTPs. The findings revealed a stepwise decrease in DMF and chemical oxygen demand (COD) content with the progression of treatment. However, the number and abundances of ARGs increase in the effluents of biological treatments. Furthermore, the residues of DMF and the treatment process altered the structure of the bacterial community. The correlation analysis indicated that the shift in bacterial community structures might be the main driver for the dynamics change of ARGs. Interestingly, observed that the AAO process may acted as a microbial source and increased the total abundance of ARGs instead of attenuating it. Additionally, found that non-pathogenic bacteria had higher ARGs abundance than pathogenic bacteria in effluents. The study provides insights into the microbial community structure and the mechanisms that drive the variation in ARGs abundance in industrial WWTPs.

Dropper failure seriously threatens the operation safety of a high-speed railway. In this work, for a simple chain suspension catenary, one span with five droppers is performed to establish a model and thus the effects of the moving load speed on dropper stress are investigated. First, the partial differential vibration equation of dropper is obtained through the mechanical analysis and converted into the finite difference equation. Then, we consider contact line as a beam element to obtain its motion equation. Furthermore, the boundary and initial conditions of five droppers are determined. Finally, the stresses of five droppers are numerically calculated and the effects of the moving load speed on dropper stress are investigated by writing a MATLAB code. The results suggest that the dropper location significantly affects its stress. Compared with other droppers, droppers II and IV have much more severe vibration amplitudes. Different moving load speeds could cause different stress change of each dropper. With the increasing speed, dropper experiences longer bending compression stage and the bending amplitude increases. The impact of the moving load speed on dropper stress is significant.

Abstract High crosslinking and low shrinkage stress are difficult to reconcile in the preparation of performance-enhancing photopolymer materials. Here we report the unique mechanism of upconversion particles-assisted NIR polymerization (UCAP) in reducing shrinkage stress and enhancing mechanical properties of cured materials. The excited upconversion particle emit UV-vis light with gradient intensity to the surroundings, forming a domain-limited gradient photopolymerization centered on the particle, and the photopolymer grows within this domain. The curing system remains fluid until the percolated photopolymer network is formed and starts gelation at high functional group conversion, with most of the shrinkage stresses generated by the crosslinking reaction having been released prior to gelation. Longer exposures after gelation contribute to a homogeneous solidification of cured material, and polymer materials cured by UCAP exhibit high gel point conversion, low shrinkage stress and strong mechanical properties than those cured by conventional UV polymerization techniques.

The density peak clustering algorithm (DPC) requires manual determination of cluster centers, and poor performance on complex datasets with varying densities or non-convexity. Hence, a novel density peak clustering algorithm is proposed for the automatic selection of clustering centers based on K-nearest neighbors (AKDPC). First, the AKDPC classifies samples according to their mutual K-nearest neighbor values into core and non-core points. Second, the AKDPC uses the average distance of K nearest neighbors of a sample as its density. The smaller the average distance is, the higher the density. Subsequently, it selects the highest density sample among all unclassified core points as a center of the new cluster, and the core points that satisfy the merging condition are added to the cluster until no core points satisfy the condition. Afterwards, the above steps are repeated to complete the clustering of all core points. Lastly, the AKDPC labels the unclassified non-core points similar to the nearest points that have been classified. In addition, to prove the validity of AKDPC, experiments on manual and real datasets are conducted. By comparing the AKDPC with classical clustering algorithms and excellent DPC-variants, this paper demonstrates that AKDPC presents higher accuracy.

Human enteroviruses (HEVs) include many different types that cause a wide range of diseases, and an effective method of genus-level identification has therefore significant clinical implications. However, quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), the gold-standard method, still has shortfalls in diagnostic sensitivity and timeliness. Here we established a one-step real-time reverse-transcription recombinase-aided PCR assay (RT-RAP) to detect HEV fragment within an hour. The RT-RAP assay showed a detection limit of 5 copies/μL using recombinant plasmids and was extensively verified using 15 HEV strains. Among 15 types of HEV (species A-C), the sensitivity of RT-RAP was approximately 2–8 folds lower than that of the qRT-PCR in 9 types, and no-cross reaction with other viruses was observed. RT-RAP was further applied to analyze CSF and fecal specimens; the clinical performance demonstrated that the RT-RAP and the commercial qRT-PCR kit provided consistent results. These results indicated that RT-RAP assay may be a promising approach for rapid and sensitive detection of HEV.

Abstract In the three-dimensional printing process of ceramic with low-angle structures, additional supporting structures are usually employed to avoid collapse of overhanging parts. However, the extra supporting structures not only affect printing efficiency, but the problems caused by their removal are also a matter of concern. Herein, we present a ceramic printing method, which can realize printing of unsupported multi-scale and large-span ceramics through the combination of direct ink writing and near-infrared induced up-conversion particles-assisted photopolymerization. This printing technology enables in-situ curing of multi-scale filaments with diameters ranging from 410 µm to 3.50 mm, and ceramic structures of torsion spring, three-dimensional bending and cantilever beam were successfully constructed through unsupported printing. This method will bring more innovation to the unsupported 3D manufacturing of complex shape ceramics.

In the global apiculture industry, reward feeding and supplementary feeding are essential for maintaining bee colonies. Beekeepers provide artificial supplements to their colonies, typically in the form of either a honey–water solution or sugar syrup. Owing to cost considerations associated with beekeeping, most beekeepers opt for sugar syrup. However, the effects of different types of artificial sugar supplements on bee colonies and their subsequent impact on honey composition remain unclear. To address this gap, this study compared the chemical composition, antioxidant capacity, and nutritional potency of three types of honey: honey derived from colonies fed sugar syrup (sugar-based product, SP) or a honey–water solution (honey-sourced honey, HH) and naturally sourced honey (flower-sourced honey, FH), which served as the control. The results revealed that FH outperformed HH and SP in terms of total acidity, sugar content, total protein content, and antioxidant capacity, and HH outperformed SP. Regarding nutritional efficacy, including the lifespan and learning and memory capabilities of worker bees, FH exhibited the best outcomes, with no significant differences observed between HH and SP. This study underscores the importance of sugar source selection in influencing honey quality and emphasizes the potential consequences of substituting honey with sugar syrup in traditional apiculture practices.

ABSTRACT: In this study, 2 types of drinking water were provided to broiler chicks to evaluate the relationship between the bacterial load of drinking water and cecal microbiota. One type of drinking water was untreated, while the other type was daily treated with sodium dichlorocyanurate (50 mg/L). A total of 240 broiler chicks were divided into 2 groups based on their initial body weight. There were 6 replicates in each group, and each replicate cage contained 20 birds. Each cage was assigned to a different floor of the battery cage. On the final day, water samples were collected from each replicate cage at the opening of the drinking cup height, and one bird was selected from each replicate cage to obtain cecal content samples for measuring microbiota composition using the 16S rRNA technique. We found that drinking water treated with sodium dichlorocyanurate significantly reduced the richness and diversity of microbiota and diminished/disappeared most gram-negative bacteria. Broiler chicks that consumed chlorinated drinking water exhibited changes in the composition of cecal microbiota, with Alistipes serving as the marker species in the cecal content of broiler chicks that consumed untreated water, whereas AF12 served as the marker species in the cecal content of broiler chicks that consumed chlorinated drinking water. Functional prediction using the MetaCyc database and species composition analysis of metabolic pathways showed that changes in 7 metabolic pathways were related to the abundance of Providencia. Therefore, we concluded that chlorinated drinking water reduced the bacterial load in drinking water, thereby altering the cecal microbiota composition and regulating the metabolic activity of broiler chicks.

AbstractThe rising number of individuals diagnosed with type 2 diabetes is a global health concern. This chronic disease can lead to a diverse range of complications. In this study, we assessed the extracorporeal hypoglycemic mechanism as determined by the inhibitory activity of TGE (Fagopyrum tataricum grain crude extract) and TBE (Fagopyrum tataricum bran crude extract) on elevated blood glucose, oxidative stress and inflammatory response. In addition, to characterize hypoglycemic activity in vivo, we investigated the anti-diabetic effects of water—ethanol extracts of Fagopyrum tataricum grain and bran with flavonoid-rich and D-CI fractions in type 2 diabetic mice induced by streptozotocin and a high-fat/high-sugar diet. Moreover, compared with the TBE group through kit detection and in vitro test analysis, treatment of mice with TGE has a better ability of antioxidant activity, Reduce inflammatory overreaction, hypoglycemia, improving symptoms of type 2 diabetic mice and reducing organ damage. In conclusion, these findings yet indicate that TGE could be used to manage the disease, for the improvement of blood glucose level and exhibits antioxidant properties for type 2 diabetes. By providing a comprehensive review of the molecular mechanisms underlying glucose metabolism, oxidative stress, inflammation, organ structural damage, and insulin resistance in individuals with type 2 diabetes mellitus. Our findings not only offer a deeper understanding of these processes but also provide novel insights and theoretical guidance for the treatment of diabetes mellitus.

ABSTRACTDisruption of the cell cycle is a common strategy shared by many viruses to create a conducible cellular microenvironment for their efficient replication. We have previously shown that infection of cells with gammacoronavirus infectious bronchitis virus (IBV) activated the theataxia-telangiectasia mutated (ATM) Rad3-related (ATR)/checkpoint kinase 1 (Chk1) pathway and induced cell cycle arrest in S and G2/M phases, partially through the interaction of nonstructural protein 13 (nsp13) with the p125 catalytic subunit of DNA polymerase delta (pol δ). In this study, we show, by GST pulldown, co-immunoprecipitation and immunofluorescent staining, that IBV nsp12 directly interacts with the p50 regulatory subunit of pol δ in vitro and in cells overexpressing the two proteins as well as in cells infected with a recombinant IBV harbouring an HA-tagged nsp12. Furthermore, nsp12 from severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 was also able to interact with p50. These interactions play a synergistic role with nsp13 in the induction of S phase arrest. The fact that subunits of an essential cellular DNA replication machinery physically associate with two core replication enzymes from three different coronaviruses highlights the importance of these associations in coronavirus replication and virus-host interaction, and reveals the potential of targeting these subunits for antiviral intervention.