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7 results on '"Xiong, Xi-Kun"'

3. The Combination of Chrysin and Cisplatin Induces Apoptosis in HepG2 through Down-regulation of cFLIP and Activity of Caspase

Author

Luo, Yang-Xin, Peng, Bao-Ying, Chen, Zheng-Hao, Xiong, Xi-Kun, Huang, Jun-Ming, Chen, Mei-Fen, Wang, Feng-Yan, Li, Xin, and Wang, Jian-Ning

Abstract

Abstract: Aim: The study aims to investigate the combined effects of chrysin and cisplatin on hepatoma(HepG2) cell lines in vivo and in vitro. Objective: Studies have suggested that chrysin can enhance the sensitivity of tumor cells to apoptosis. Drug resistance in tumor cells reduced the effectiveness of chemotherapy drugs such as cisplatin. We investigated whether the combination of chrysin and cisplatin can induce more apoptosis than chrysin alone and cisplatin alone. Methods: HepG2 cells were pretreated with chrysin for 2 h, followed by the addition of cisplatin for another 24 h. The morphologic changes were observed under inverted microscope and the cell viability was measured using the MTT test. The protein and cleavage of caspase-3,8,9, PARP, and cFLIP were determined by Western blotting. Results: The cell viability of the HepG2 cell can be reduced by the combination of chrysin pretreatment for 2 h and cisplatin addition for 24 h; Caspase-3,8,9 and PARP were cleaved after 12 h treatment with chrysin and cisplatin; Pancaspase inhibitor, Z-VAD-fmk, could reverse the apoptosis induced by chrysin and cisplatin in HepG2 cells; cFLIP was down-regulated by the combination of chrysin and cisplatin, and could be reversed by Z-VAD-fmk; the xenografted HepG2 cells formed a tumor in one week; At the end of the experiment, there were significant differences in relative tumor volume (RTV) and relative tumor proliferation rate between the combined group and the control group, the chrysin group and the cisplatin group; Western blotting showed that the levels of PARP, cFLIP, and caspase-3 proteins in isolated tumor tissues also decreased under the combined action of chrysin and cisplatin. Conclusion: The combination of chrysin and cisplatin induces apoptosis of hepatic tumor in vivo and in vitro. It downregulates cFLIP and then activates caspase-8, which triggers caspase-mediated apoptosis of HepG2 cell.

Published
2023
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6. Prediction of Ocular Irritancy of 26 Chemicals and 26 Cosmetic Products with Isolated Rabbit Eye (IRE) Test.

Author

GUO, Xiang, YANG, Xing Fen, YANG, Ying, HANS, Raabe, CAI, Jing Heng, XUE, Jin Yu, TAN, Xiao Hua, XIE, Xiao Ping, XIONG, Xi Kun, and HUANG, Jun Ming

Abstract

Abstract: Objective: This study aims to establish and evaluate the methodology of isolated rabbit eye (IRE) test. Methods: IRE test was performed according to modifications of the in vitro toxicology (INVITTOX) Protocol No.85: Rabbit enucleated eye test by European Centre for the Validation of Alternative Methods (ECVAM), and then 26 chemicals and 26 cosmetic products were tested in both in vitro IRE and in vivo Draize tests. A statistical analysis was conducted to determine the relevance of the IRE test to the data generated in the Draize test. Results: IRE test was established successfully in our laboratory. It was shown that ranking correlation and class concordance were fairly well between the IRE test and the Draize test for 26 reference chemicals (Fisher''s Exact Test χ2=51.314, P<0.001; McNemar P=0.261; Gamma=0.960, P<0.001; Kappa=0.843, P<0.001) and 26 cosmetic products (Fisher''s Exact Test χ2=15.522, P<0.001; McNemar P=0.311; Gamma=0.967, P<0.001; Kappa=0.611, P<0.001). Conclusion: IRE test was established successfully for in vitro testing of eye irritation as an alternative to Draize test. [Copyright &y& Elsevier]

Published
2012
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7. [The establishment and assessment on cosmetic products using 3T3 mouse fibroblast neutral red uptake phototoxicity assay].

Author

Yang Y, Xiong XK, Yang XF, He XW, Huang JM, Tan XH, and Zheng SS

Subjects
3T3 Cells drug effects, Animals, Animals, Newborn, Guinea Pigs, Mice, Toxicity Tests, Cosmetics toxicity, Dermatitis, Phototoxic, Fibroblasts drug effects
Abstract

Objective: To establish the 3T3 mouse fibroblast neutral red uptake (NRU-PT) phototoxicity test method, and evaluate the practicality of the method in detecting potential phototoxicity of the cosmetic products., Methods: Fifteen phototoxic and 9 non-phototoxic chemicals were tested in our laboratories, the phototoxic potential of the test chemicals was evaluated in a prediction model in which either the photo irritation factor (PIF) or the mean photo effect (MPE) was compared with the coherence and sensitivity of the method. 20 kinds of functional cosmetics were detected and the results were analyzed by the 3T3 NRU-PT in vitro and Guinea pig skin phototoxicity test (in vivo)., Results: Both PIF and MPE of the chemicals were highly reproduced, and the correlation between in vitro and in vivo data was almost perfect. All the non-phototoxic provided a negative result, while 14 of the 15 phototoxic tested chemicals gave clear positive results. For cosmetics, the correlation between in vitro and in vivo data was consistent., Conclusion: The 3T3 NRU PT test was established successfully, it should be used as a good alternative method for assessing the phototoxic potential of the chemicals and cosmetics in China.

Published
2007

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