27 results on '"Xiongbing Lu"'
Search Results
2. Urothelium with barrier function differentiated from human urine-derived stem cells for potential use in urinary tract reconstruction
- Author
-
Qian Wan, Geng Xiong, Guihua Liu, Thomas D. Shupe, Guanghui Wei, Deying Zhang, Dan Liang, Xiongbing Lu, Anthony Atala, and Yuanyuan Zhang
- Subjects
Urothelium ,Urine-derived stem cells ,Barrier function ,Tight junctions ,Bladder diseases ,Tissue engineering ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Autologous urothelial cells are often obtained via bladder biopsy to generate the bio-engineered urethra or bladder, while urine-derived stem cells (USC) can be obtained by a non-invasive approach. The objective of this study is to develop an optimal strategy for urothelium with permeability barrier properties using human USC which could be used for tissue repair in the urinary tract system. Methods USC were harvested from six healthy adult individuals. To optimize urothelial differentiation, five different differentiation methods were studied. The induced cells were assessed for gene and protein expression markers of urothelial cells via RT-PCR, Western blotting, and immunofluorescent staining. Barrier function and ultrastructure of the tight junction were assessed with permeability assays and transmission electron microscopy (TEM). Induced cells were both cultured on trans-well membranes and small intestinal submucosa, then investigated under histology analysis. Results Differentiated USC expressed significantly higher levels of urothelial-specific transcripts and proteins (Uroplakin III and Ia), epithelial cell markers (CK20 and AE1/AE3), and tight junction markers (ZO-1, ZO-2, E-cadherin, and Cingulin) in a time-dependent manner, compared to non-induced USC. In vitro assays using fluorescent dye demonstrated a significant reduction in permeability of differentiated USC. In addition, transmission electron microscopy confirmed appropriate ultrastructure of urothelium differentiated from USC, including tight junction formation between neighboring cells, which was similar to positive controls. Furthermore, multilayered urothelial tissues formed 2 weeks after USC were differentiated on intestine submucosal matrix. Conclusion The present study illustrates an optimal strategy for the generation of differentiated urothelium from stem cells isolated from the urine. The induced urothelium is phenotypically and functionally like native urothelium and has proposed uses in in vivo urological tissue repair or in vitro urethra or bladder modeling.
- Published
- 2018
- Full Text
- View/download PDF
3. Human Urine‐Derived Stem Cell Differentiation to Endothelial Cells with Barrier Function and Nitric Oxide Production
- Author
-
Guihua Liu, Rongpei Wu, Bin Yang, Chunhua Deng, Xiongbing Lu, Stephen J. Walker, Peter X. Ma, Steve Mou, Anthony Atala, and Yuanyuan Zhang
- Subjects
Endothelial differentiation ,Urine derived stem cells ,Stem cells transplantation ,Angiogenesis ,Autologous stem cells ,Medicine (General) ,R5-920 ,Cytology ,QH573-671 - Abstract
Abstract Endothelial cells (ECs) play a key role in revascularization within regenerating tissue. Stem cells are often used as an alternative cell source when ECs are not available. Several cell types have been used to give rise to ECs, such as umbilical cord vessels, or differentiated from somatic stem cells, embryonic, or induced pluripotent stem cells. However, the latter carry the potential risk of chronic immune rejection and oncogenesis. Autologous endothelial precursors are an ideal resource, but currently require an invasive procedure to obtain them from the patient's own blood vessels or bone marrow. Thus, the goal of this study was to determine whether urine‐derived stem cells (USCs) could differentiate into functional ECs in vitro. Urine‐derived cells were then differentiated into cells of the endothelial lineage using endothelial differentiation medium for 14 days. Changes in morphology and ultrastructure, and functional endothelial marker expression were assessed in the induced USCs in vitro. Grafts of the differentiated USCs were then subcutaneously injected into nude mice. Induced USCs expressed significantly higher levels of specific markers of ECs (CD31, vWF, eNOS) in vitro and in vivo, compared to nondifferentiated USCs. In addition, the differentiated USC formed intricate tubular networks and presented similar tight junctions, and migration and invasion ability, as well as ability to produce nitric oxide (NO) compared to controls. Using USCs as autologous EC sources for vessel, tissue engineering strategies can yield a sufficient number of cells via a noninvasive, simple, and low‐cost method suitable for rapid clinical translation. Stem Cells Translational Medicine 2018 Stem Cells Translational Medicine 2018;7:686–698
- Published
- 2018
- Full Text
- View/download PDF
4. Analysis of repeated renal arteriography after percutaneous nephrolithotomy
- Author
-
Zhaohui, He, Hanqi, Lei, Xiongbing, Lu, Caixia, Zhang, Shawpong, Wan, and Guohua, Zeng
- Published
- 2017
- Full Text
- View/download PDF
5. Genome-wide screening for the G-protein-coupled receptor (GPCR) pathway-related therapeutic gene RGS19 (regulator of G protein signaling 19) in bladder cancer
- Author
-
Sainan Duan, Yize Zhang, Bing Ding, Jin Kuang, Cong Wang, Ying Deng, Yue Liu, Guang Chen, Xiongbing Lu, and Weiming Lou
- Subjects
Indoles ,Cell ,Bioengineering ,Biology ,g-protein-coupled receptor ,Applied Microbiology and Biotechnology ,Regulator of G protein signaling ,Cell Line, Tumor ,Databases, Genetic ,medicine ,Humans ,Receptor ,Protein Kinase Inhibitors ,Gene ,Cell Proliferation ,G protein-coupled receptor ,Aza Compounds ,Bladder cancer ,Cell Cycle ,Cancer ,General Medicine ,gsk1070916 ,Cell cycle ,Prognosis ,medicine.disease ,medicine.anatomical_structure ,Urinary Bladder Neoplasms ,Cancer research ,rgs19 ,TP248.13-248.65 ,RGS Proteins ,Research Article ,Research Paper ,Signal Transduction ,Biotechnology - Abstract
Bladder cancer is one of the most severe genitourinary cancers, causing high morbidity worldwide. However, the underlying molecular mechanism is not clear, and it is urgent to find target genes for treatment. G-protein-coupled receptors are currently a target of high interest for drug design. Thus, we aimed to identify a target gene-related to G-protein-coupled receptors for therapy. We used The Cancer Genome Atlas (TCGA) and DepMap databases to obtain the expression and clinical data of RGS19. The results showed that RGS19 was overexpressed in a wide range of tumor, especially bladder cancer. We also explored its effect on various types of cancer. High expression of RGS19 was also shown to be significantly associated with poor prognosis. Cell models were constructed for cell cycle detection. shRGS19 can halt the cell cycle at a polyploid point. RGS19 is a G-protein-coupled receptor signaling pathway-related gene with a significant effect on survival. We chose RGS19 as a therapeutic target gene in bladder cancer. The drug GSK1070916 was found to inhibit the effect of RGS19 via cell rescue experiments in vitro.
- Published
- 2021
6. Endovascular removal of a misplaced ureteral stent in the vena cava: a complication of ultrasound‐guided percutaneous nephrolithotomy
- Author
-
Xiongbing Lu, Xianhua Zhu, Chao Hu, Anyi Zhu, and Ting Liang
- Subjects
Interventional therapy ,medicine.medical_specialty ,Medicine (General) ,Complications ,Vena cava ,Intravascular intervention ,medicine.medical_treatment ,Case Report ,Case Reports ,Inferior vena cava ,R5-920 ,medicine ,cardiovascular diseases ,Percutaneous nephrolithotomy ,business.industry ,Stent ,General Medicine ,Ultrasound guided ,Surgery ,Double J stent ,medicine.vein ,Double j stent ,Medicine ,Complication ,business - Abstract
Intravascular migration of a double J stent into the inferior vena cava is an uncommon complication. Active prevention, timely diagnosis, and early intervention are crucial for this complication. Intravascular interventional therapy is relatively easy, less traumatic, and has a high success rate. It can be used to select patients for intravascular ectopic DJS treatment., Intravascular interventional therapy can be used to select patients to retrieve the ectopic intravascular DJS because it is relatively easy, less invasive, and has a high success rate.
- Published
- 2021
7. Analytical factors and treatment methods of renal parenchyma perforation after ureteral double-J stenting
- Author
-
Fei Liu, Zhihua Zeng, Lingxing Duan, Wanming Zhou, and Xiongbing Lu
- Subjects
medicine.medical_specialty ,Text mining ,Ureter ,medicine.anatomical_structure ,business.industry ,X ray computed ,Renal parenchyma ,Perforation (oil well) ,medicine ,Treatment method ,Surgery ,Radiology ,business - Published
- 2019
8. Human Urine-Derived Stem Cell Differentiation to Endothelial Cells with Barrier Function and Nitric Oxide Production
- Author
-
Stephen J. Walker, Rongpei Wu, Anthony Atala, Bin Yang, Steve Mou, Xiongbing Lu, Yuanyuan Zhang, Peter X. Ma, Chunhua Deng, and Guihua Liu
- Subjects
Male ,0301 basic medicine ,CD31 ,Vascular Regeneration ,Angiogenesis ,Cellular differentiation ,Urine ,Mice ,0302 clinical medicine ,Translational Research Articles and Reviews ,Cell Movement ,Angiogenic Proteins ,Induced pluripotent stem cell ,lcsh:R5-920 ,lcsh:Cytology ,Chemistry ,Stem Cells ,Cell Differentiation ,General Medicine ,Middle Aged ,Urine derived stem cells ,3. Good health ,Cell biology ,Lipoproteins, LDL ,Drug Combinations ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Antigens, Surface ,Stem cells transplantation ,Proteoglycans ,Collagen ,Stem cell ,lcsh:Medicine (General) ,Adult stem cell ,Adult ,Mice, Nude ,Neovascularization, Physiologic ,Nitric Oxide ,Tight Junctions ,03 medical and health sciences ,Tissue Engineering and Regenerative Medicine ,medicine ,Animals ,Humans ,lcsh:QH573-671 ,Endothelial Cells ,Mesenchymal Stem Cells ,Endothelial differentiation ,Cell Biology ,Embryonic stem cell ,030104 developmental biology ,Laminin ,Bone marrow ,Autologous stem cells ,Developmental Biology - Abstract
Endothelial cells (ECs) play a key role in revascularization within regenerating tissue. Stem cells are often used as an alternative cell source when ECs are not available. Several cell types have been used to give rise to ECs, such as umbilical cord vessels, or differentiated from somatic stem cells, embryonic, or induced pluripotent stem cells. However, the latter carry the potential risk of chronic immune rejection and oncogenesis. Autologous endothelial precursors are an ideal resource, but currently require an invasive procedure to obtain them from the patient's own blood vessels or bone marrow. Thus, the goal of this study was to determine whether urine‐derived stem cells (USCs) could differentiate into functional ECs in vitro. Urine‐derived cells were then differentiated into cells of the endothelial lineage using endothelial differentiation medium for 14 days. Changes in morphology and ultrastructure, and functional endothelial marker expression were assessed in the induced USCs in vitro. Grafts of the differentiated USCs were then subcutaneously injected into nude mice. Induced USCs expressed significantly higher levels of specific markers of ECs (CD31, vWF, eNOS) in vitro and in vivo, compared to nondifferentiated USCs. In addition, the differentiated USC formed intricate tubular networks and presented similar tight junctions, and migration and invasion ability, as well as ability to produce nitric oxide (NO) compared to controls. Using USCs as autologous EC sources for vessel, tissue engineering strategies can yield a sufficient number of cells via a noninvasive, simple, and low‐cost method suitable for rapid clinical translation. Stem Cells Translational Medicine 2018 Stem Cells Translational Medicine 2018;7:686–698
- Published
- 2018
9. PRMT7 promotes the growth of renal cell carcinoma through modulating the β-catenin/C-MYC axis
- Author
-
Haibin Zou, Lisong Wan, Fei Liu, Zhengyue Pan, Wanming Zhou, and Xiongbing Lu
- Subjects
0301 basic medicine ,Male ,Protein-Arginine N-Methyltransferases ,Methyltransferase ,Carcinogenesis ,Mice, Nude ,Cell Growth Processes ,Biochemistry ,Proto-Oncogene Proteins c-myc ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Renal cell carcinoma ,medicine ,Animals ,Humans ,Carcinoma, Renal Cell ,beta Catenin ,Mice, Inbred BALB C ,Oncogene ,Chemistry ,Cell growth ,Cancer ,Cell Biology ,Methylation ,Middle Aged ,medicine.disease ,Prognosis ,Kidney Neoplasms ,Clear cell renal cell carcinoma ,030104 developmental biology ,030220 oncology & carcinogenesis ,Catenin ,Cancer research ,Heterografts - Abstract
Arginine methylation is mainly catalyzed by protein arginine methyltransferases (PRMTs) and is one of the most common posttranslational modifications closely related to the development of cancer. PRMT7 is overexpressed in various tumors and promotes the malignant progression of tumors, but the expression and role of PRMT7 in renal cell carcinoma (RCC) remains unclear. Here, we report for the first time that the expression of PRMT7 is increased in clear cell renal cell carcinoma (ccRCC) tissues and that it may act as an independent predictor for the poor prognosis of ccRCC patients. We found that PRMT7 promotes RCC cell proliferation both in vitro and in vivo. Moreover, the methyltransferase inhibitor adenosine dialdehyde (Adox) blocks the action of PRMT7 in ccRCC cells. Furthermore, PRMT7 regulates the expression of C-MYC, which plays an important role in promoting ccRCC cell proliferation, and it accelerates the tumor development of RCC in a C-MYC-dependent manner. Mechanistically, PRMT7 upregulates the expression of C-MYC via methylating β-catenin and inhibiting the ubiquitin-mediated degradation of β-catenin. In conclusion, our study demonstrates that overexpressed PRMT7 in ccRCC cells acts as an oncogene to promote the growth of renal cell carcinoma through regulating the β-catenin/C-MYC axis, thereby providing new strategies and targets for the treatment of ccRCC patients.
- Published
- 2019
10. Urothelium with barrier function differentiated from human urine-derived stem cells for potential use in urinary tract reconstruction
- Author
-
Thomas Shupe, Guanghui Wei, Dan Liang, Geng Xiong, Deying Zhang, Yuanyuan Zhang, Xiongbing Lu, Guihua Liu, Qian Wan, and Anthony Atala
- Subjects
Adult ,Male ,0301 basic medicine ,Cell Membrane Permeability ,Swine ,Bladder diseases ,Urinary system ,Urine-derived stem cells ,Medicine (miscellaneous) ,Urine ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Barrier function ,lcsh:Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Humans ,Tissue engineering ,lcsh:QD415-436 ,Urothelium ,Urinary Tract ,Cell Shape ,Tight junctions ,Cell Proliferation ,lcsh:R5-920 ,Tight junction ,Chemistry ,Research ,Stem Cells ,Cell Differentiation ,Histology ,Cell Biology ,Middle Aged ,Molecular biology ,Epithelium ,Cingulin ,030104 developmental biology ,medicine.anatomical_structure ,Molecular Medicine ,Collagen ,Stem cell ,lcsh:Medicine (General) ,Biomarkers ,030217 neurology & neurosurgery - Abstract
Background Autologous urothelial cells are often obtained via bladder biopsy to generate the bio-engineered urethra or bladder, while urine-derived stem cells (USC) can be obtained by a non-invasive approach. The objective of this study is to develop an optimal strategy for urothelium with permeability barrier properties using human USC which could be used for tissue repair in the urinary tract system. Methods USC were harvested from six healthy adult individuals. To optimize urothelial differentiation, five different differentiation methods were studied. The induced cells were assessed for gene and protein expression markers of urothelial cells via RT-PCR, Western blotting, and immunofluorescent staining. Barrier function and ultrastructure of the tight junction were assessed with permeability assays and transmission electron microscopy (TEM). Induced cells were both cultured on trans-well membranes and small intestinal submucosa, then investigated under histology analysis. Results Differentiated USC expressed significantly higher levels of urothelial-specific transcripts and proteins (Uroplakin III and Ia), epithelial cell markers (CK20 and AE1/AE3), and tight junction markers (ZO-1, ZO-2, E-cadherin, and Cingulin) in a time-dependent manner, compared to non-induced USC. In vitro assays using fluorescent dye demonstrated a significant reduction in permeability of differentiated USC. In addition, transmission electron microscopy confirmed appropriate ultrastructure of urothelium differentiated from USC, including tight junction formation between neighboring cells, which was similar to positive controls. Furthermore, multilayered urothelial tissues formed 2 weeks after USC were differentiated on intestine submucosal matrix. Conclusion The present study illustrates an optimal strategy for the generation of differentiated urothelium from stem cells isolated from the urine. The induced urothelium is phenotypically and functionally like native urothelium and has proposed uses in in vivo urological tissue repair or in vitro urethra or bladder modeling. Electronic supplementary material The online version of this article (10.1186/s13287-018-1035-6) contains supplementary material, which is available to authorized users.
- Published
- 2018
11. ARHGAP24 inhibits cell cycle progression, induces apoptosis and suppresses invasion in renal cell carcinoma
- Author
-
Jie Fan, Tianlun Huang, Gaosi Xu, and Xiongbing Lu
- Subjects
Adult ,Male ,0301 basic medicine ,Nephrology ,medicine.medical_specialty ,Pathology ,proliferation ,renal cancer ,Mice, Nude ,Apoptosis ,urologic and male genital diseases ,Metastasis ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Renal cell carcinoma ,Cell Line, Tumor ,Internal medicine ,medicine ,Animals ,Humans ,Genes, Tumor Suppressor ,Neoplasm Invasiveness ,Carcinoma, Renal Cell ,Aged ,Aged, 80 and over ,Kidney ,business.industry ,Cell growth ,Cell Cycle ,GTPase-Activating Proteins ,Cancer ,ARHGAP24 ,Middle Aged ,Cell cycle ,medicine.disease ,Kidney Neoplasms ,HEK293 Cells ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Female ,business ,Cell Division ,Research Paper - Abstract
// Gaosi Xu 1, * , Xiongbing Lu 2, * , Tianlun Huang 1 , Jie Fan 1 1 Department of Nephrology, The Second Affiliated Hospital of Nanchang University, Nanchang, China 2 Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang, China * These authors have contributed equally to this work Correspondence to: Gaosi Xu, email: gaosixu325@163.com Keywords: renal cancer, ARHGAP24, proliferation, cell cycle, apoptosis Received: March 14, 2016 Accepted: June 13, 2016 Published: July 2, 2016 ABSTRACT Renal cell carcinoma (RCC) is the major cause of kidney malignancy-related deaths. Rho GTPases are key regulators in cancer cell metastasis. ARHGAP24, a Rac-specific member of the Rho GTPase-activating protein family, acts as a functional target of cancer cell migration and invasion. In the present study, we identified ARHGAP24 expression is downregulated in renal cancer tissues and is highly correlated with long-term survival in RCC patients. Therefore, we investigated the biological functions of ARHGAP24 in renal cancer cells. Ectopic expression of ARHGAP24 resulted in inhibited cell proliferation and arrested cell cycle in two renal cancer cell lines (786-0 and Caki-2); the results were confirmed by ARHGAP24 knocking down. In addition, ARHGAP24 significantly reduced the cell invasion ability and induced apoptosis in renal cancer cells. In addition, overexpressing ARHGAP24 impaired tumor formation in vivo . In summary, our results illustrated that ARHGAP24 plays a unique role in RCC progression as a tumor repressor.
- Published
- 2016
12. Fibroblasts from pBOO promote tumorigenesis by secreting TGFbeta1 to induce EMT in bladder urothelial carcinoma cells
- Author
-
Zhihua Zeng, Jian Zhang, Lingxing Duan, Xiongbing Lu, and Renrui Kuang
- Subjects
0301 basic medicine ,Bladder cancer ,Bladder Urothelial Carcinoma ,030102 biochemistry & molecular biology ,biology ,Chemistry ,Biophysics ,Cell Biology ,medicine.disease ,medicine.disease_cause ,Biochemistry ,Metastasis ,03 medical and health sciences ,Bladder outlet obstruction ,Gene expression ,biology.protein ,medicine ,Cancer research ,Epithelial–mesenchymal transition ,Neutralizing antibody ,Carcinogenesis ,Molecular Biology - Abstract
Urinary bladder cancer (UBC) is one of the most common malignancies worldwide. UBC patients at muscle invasive stage have poor clinical outcome, due to high propensity for metastasis. Non-tumor activated fibroblasts, named α-SMA+Fs, is similar to carcinoma-associated fibroblasts (CAFs) which could express α-SMA. However, whether α-SMA+Fs patients could induce UBC cell invasion is unclear. Herein, we found that characterization of primary α-SMA+Fs separated from PBOO (partial bladder outlet obstruction) rats was fell in between normal fibroblasts (α-SMA-Fs) and CAFs. Additionally, the conditional medium from α-SMA+Fs enhanced the NBT-II cell invasion through inducing EMT, and the oncogenic function of mixed supernatant of α-SMA+Fs/CAFs was stronger than that of CAFs. Inhibition of TGF-β1 by TGF-β1 neutralizing antibody decreased the EMT-associated gene expression and NBT-II cell invasion, suggesting that α-SMA+Fs can induce tumor EMT through TGF-β1. Xenograft experiments showed that the tumorigenic effect of α-SMA+Fs in mice was also between CAFs and α-SMA-Fs, and α-SMA+Fs/CAFs also had a strong tumorigenic effect. We preformed rats with PBOO and found that the incidence of invasive bladder cancer in PBOO+BBN group was higher than in BBN group, suggesting the PBOO treatment contributed to tumorigenesis. Thus, α-SMA+Fs promoted tumorigenesis by secreting TGF-β1 to induce EMT.
- Published
- 2018
13. Circular RNA circ-VANGL1 as a competing endogenous RNA contributes to bladder cancer progression by regulating miR-605-3p/VANGL1 pathway
- Author
-
Liang Jin, Lingxing Duan, Xiongbing Lu, Wanming Zhou, Zhihua Zeng, Yi Yu, and Jian Zhang
- Subjects
0301 basic medicine ,Male ,Physiology ,Clinical Biochemistry ,Cell ,Mice, Nude ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Circular RNA ,Cell Movement ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Neoplasm Invasiveness ,Cell Proliferation ,Gene knockdown ,Mice, Inbred BALB C ,Competing endogenous RNA ,Cell growth ,Membrane Proteins ,Cell Biology ,Cell Cycle Checkpoints ,RNA, Circular ,Cell cycle ,Middle Aged ,In vitro ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Urinary Bladder Neoplasms ,030220 oncology & carcinogenesis ,Cancer research ,Disease Progression ,Female ,Signal transduction ,Carrier Proteins ,Signal Transduction - Abstract
Increasing reports indicate that circular RNAs (circRNAs) are very important regulators in human diseases, including cancers. In bladder cancer (BC), several circRNAs have been reported to be involved in tumor progressions, such as circ-ITCH and circTCF25. However, the functions of most circRNAs in BC still remains largely unknown. In this study, we identified a novel circRNA termed as circ-VANGL1 by bioinformatics analysis. We found that circ-VANGL1 was highly expressed in BC tissues compared with adjacent normal tissues. Furthermore, we showed that circ-VANGL1 could serve as a prognostic marker for patients with BC. Through functional experiments, we found that circ-VANGL1 knockdown significantly suppressed BC cell proliferation, cell cycle, migration, and invasion in vitro. Besides, circ-VANGL1 silence inhibited BC cell propagation in vivo. Mechanistically, we identified circ-VANGL1 as a sponge of miR-605-3p which targeted VANGL1 in BC cells. Through repressing miR-605-3p availability, circ-VANGL1 contributes to VANGL1 expression, consequently leading to BC cell proliferation, migration, and invasion. Taken together, our study demonstrated circ-VANGL1/miR-605-3p/VANGL1 as a novel essential signaling pathway involved in BC progression.
- Published
- 2018
14. Additional file 4: of Urothelium with barrier function differentiated from human urine-derived stem cells for potential use in urinary tract reconstruction
- Author
-
Wan, Qian, Xiong, Geng, Guihua Liu, Shupe, Thomas, Guanghui Wei, Deying Zhang, Liang, Dan, Xiongbing Lu, Atala, Anthony, and Yuanyuan Zhang
- Abstract
Table S4. Percentage of USC expressing tight junction markers 2Â weeks after urothelial induction assessed by immunofluorescence. (DOCX 16Â kb)
- Published
- 2018
- Full Text
- View/download PDF
15. Urine Progenitor Cells for Potential Application in Renal Tissue Repair
- Author
-
Peng Li, Yuanyuan Zhang, Xiongbing Lu, Junhong Deng, and Andrea Peloso
- Subjects
Kidney ,Pathology ,medicine.medical_specialty ,Cell growth ,Cell ,Mesenchymal stem cell ,Biology ,Cell biology ,Paracrine signalling ,medicine.anatomical_structure ,medicine ,Progenitor cell ,Stem cell ,Induced pluripotent stem cell - Abstract
Human urine-derived stem cells (USCs), a subpopulation of cells isolated from urine, possess biological characteristics of stem cells, i.e., clonogenicity, cell growth patterns and expansion capacity, cell surface marker expression profiles, capacity for multipotent differentiation, paracrine effects, and immunomodulatory properties. USCs originate from the parietal epithelial cells in kidney glomeruli. These cells not only can differentiate into mesenchymal cell lineages, but also renal cells, including podocytes and endothelial cells. USCs possess immunomodulatory properties via inhibition of T-cell and B-cell proliferation. In vivo studies demonstrated that USCs significantly enhanced renal function via antiinflammatory and antifibrotic mechanisms after implantation of USCs in rodent models of renal function insufficiency. Importantly, USCs can be obtained via a simple, noninvasive and low-cost approach. Thus, USCs provide a very promising cell source for treatment of renal diseases.
- Published
- 2017
16. List of Contributors
- Author
-
Michael Abecassis, Ahmed Abed, Massimo Abelli, Sarwat Ahmad, Mario Alessiani, Aynaa Alsharidi, Fiorella Altruda, Gabriella Amorese, Andrea Angeletti, Mario Angelico, Roberta Angelico, Maria L. Angelotti, Robert J. Applegate, Anthony Atala, Chiara Attanasio, David Axelrod, Yanik Bababekov, Joydeep Basu, Francesca Becherucci, P. Matthew Belford, Enrico Benedetti, Valentina Benedetti, Ariela Benigni, Timothy A. Bertram, Oriol Bestard, Joshua Blake, Ugo Boggi, Lauren Brasile, Jonathan S. Bromberg, Sophie Brouard, Matthew Brovold, George W. Burke, Mirela Busic, Zeeshan Butt, Silvia Caddeo, Stefano Calzone, Josep M. Campistol, Irene Carmagnola, Fiona Carty, Diego Castanares-Zapatero, Christos E. Chadjichristos, Brooke E. Chambers, Sindhu Chandran, Christos Chatziantoniou, Ashton Chen, Linda Chen, Xiwu Chen, Valeria Chiono, Manuel Chiusa, Gaetano Ciancio, Gianluca Ciardelli, Gino Coletti, Elisabeth Coll, Christine Collienne, Robert B. Colvin, Monica Cortinovis, A. Benedict Cosimi, Paolo Cravedi, Giuseppe D’Amico, Stefano Da Sacco, Richard Danger, Jacques Dantal, Alan J. Davidson, Letizia De Chiara, Johannes W. De Fijter, Gloria de la Rosa, Francis L. Delmonico, Junhong Deng, Corinne Deurdulian, Abritee Dhal, Paolo Dionigi, Beatriz Domínguez-Gil, Marek Drozdzik, Jean-Claude Dussaule, Lauren Edgar, Maria Francesca Egidi, Hany El Hennawy, Karen English, Matthew J. Everly, Sharmila Fagoonee, Elvira Smeralda Famulari, Alan C. Farney, Marina Figliuzzi, Marialuisa Framarino-dei-Malatesta, David E. Fumo, Elena Gagliardini, Lorenzo Gallon, Sanjay K. Gandhi, Michael D. Gautreaux, Anna Geraedts, Domenico Giannese, Pier C. Giulianotti, Michael S. Goligorsky, Adam Griesemar, Josep M. Grinyó, Angelika C. Gruessner, Rainer W.G. Gruessner, Bulang He, Eliot Heher, Bing Ho, Sarah A. Hosgood, Kiyohiko Hotta, Atul Humar, H. David Humes, Giuseppe Iaria, Barbara Imberti, Juan Carlos Izpisua Belmonte, Ina Jochmans, Ravi Katari, Panagiotis Kavvadas, Tatsuo Kawai, Carlos Kengla, Tristan Keys, Amritha Kidiyoor, Kengo Kidokoro, Deepali Kumar, Michael A. Kutcher, Quirino Lai, Pierre-François Laterre, Céleste Lebbé, Christophe Legendre, Rachel Lennon, Peng Li, Jen-Jar Lin, Melissa H. Little, Xiongbing Lu, John W. Ludlow, Beatriz Mahíllo, Rosalinde Masereeuw, Rafael Matesanz, Mirjana S. Matovinovic, Benedetta Mazzinghi, Serge Cedrick Mbiandjeu Toya, Scott McEwen, Fabio Melandro, Loredana Melchiorri, Madhav C. Menon, Majid Mirzazadeh, Samantha Montag, Robert A. Montgomery, Virginie Montiel, Nuria Montserrat, Marina Morigi, Christian C. Morrill, Michel Mourad, Sean V. Murphy, Patricia Murray, Tiziana Nardo, Paolo A. Netti, Mark Nguyen, Michael L. Nicholson, John M. O’Callaghan, Linda Ohler, Giuseppe Orlando, Kenji Osafune, Tetsu Oura, Anna Peired, Andrea Peloso, Zhenzhen Peng, Norberto Perico, Laura Perin, Vittorio Perrone, Paul Persad, János Peti-Peterdi, Astgik Petrosyan, Andrea Pietrabissa, Christopher J. Pino, Jacques Pirenne, Francesco Pisani, Rutger J. Ploeg, Esteban Porrini, Ambra Pozzi, Alberto Pugliese, Luigi Pugliese, Ton J. Rabelink, Teresa Rampino, Michael A. Rees, Marlies E.J. Reinders, Andrea Remuzzi, Giuseppe Remuzzi, Anne Riquier-Brison, Raquel G. Roca, Jeffrey Rogers, Paola Romagnani, Ivy A. Rosales, Norman D. Rosenblum, Cinzia Rota, Piero Ruggenenti, Francesca Ruini, Junichiro Sageshima, Fadi El Salem, Shaifali Sandal, Veronika Sander, Ilaria Santeramo, Renato M. Santos, Minnie Sarwal, Jigesh Shah, Aneesha A. Shetty, Lorenzo Silengo, Eric Siskind, Anton Skaro, Renaud Snanoudj, Shay Soker, Andrew M. South, Goce Spasovski, Robert J. Stratta, Charles Strom, Bart Stubenitsky, Riccardo Tamburrini, Qizhi Tang, Ekamol Tantissattamo, Masayuki Tasaki, Hisham Tchelepi, Elena Ticozzelli, Opas Traitanon, Matias Trillini, Ivo Tzvetanov, María O. Valentín, Flavio Vincenti, Fabio Vistoli, Jelle Vriend, Stephen J. Walker, Jason A. Wertheim, David F. Williams, Bettina Wilm, Martijn J. Wilmer, Rebecca A. Wingert, Xavier Wittebole, Yun Xia, Christodoulos Xinaris, Kazuhiko Yamada, Takashi Yokoo, Shinya Yokote, Maarten L. Zandvliet, Roy Zent, Yuanyuan Zhang, David X. Zhao, Lihui Zhao, and Susan Y. Zhao
- Published
- 2017
17. Ectopic insertion of a duplicated ureter into prostatic urethra: Demonstration by 3D multi-detector computed tomography urography
- Author
-
Ying Liu, Xiongbing Lu, and Jun Deng
- Subjects
Adult ,Male ,medicine.medical_specialty ,Urinary system ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Ureter ,Prostatic urethra ,medicine ,Humans ,Ureteral Diseases ,Radiology, Nuclear Medicine and imaging ,Electrical and Electronic Engineering ,Instrumentation ,Radiation ,medicine.diagnostic_test ,business.industry ,Urography ,Condensed Matter Physics ,medicine.disease ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Duplicated ureter ,Maximum intensity projection ,Angiography ,Ectopic ureter ,Radiology ,business ,Tomography, X-Ray Computed ,Pyelogram - Abstract
Ectopic insertion of the ureter in the genitourinary tract is a rare congenital disorder, usually associated with ureteral duplication. Identification of the insertion open is critical for ureteric re-implantation. However, the challenge in the diagnosis of ectopic insertion of the ureter usually is to identify its insertion, particularly when the affected ureter is not dilated. Multi-detector computed tomography (MDCT) urography with nonionic iodinated contrast media delineates the ureteric course in the normal functioning kidney in the excretory phase [1]. This report presented a young male patient with ectopic insertion of a duplicated ureter diagnosed by MDCT urography. Three-dimensional (3D) analysis technology, such as volume rendering (with a color display improving the visualization of complex anatomy and 3D relationships) and maximum intensity projection (similar in principle to projection angiography), is useful for the illustration of urinary tract anatomy [1]. Rotated volume rendering reconstruction images and continual thinner maximum intensity projection reformatted images can be viewed as videos, which provides detail delineation of the ectopic ureteral insertion and its associated ureteral duplication.In this study, we reported MDCT urography and 3D analysis technology as an appropriate diagnostic method for the ectopic ureteral insertion and its associated complications.
- Published
- 2016
18. Noncontrast multidetector-row computed tomography scanning for detection of radiolucent calculi in acute renal insufficiency caused by bilateral ureteral obstruction of ceftriaxone crystals
- Author
-
Rongpei Wu, Yuanyuan Zhang, Xiongbing Lu, and Xiaoning Huang
- Subjects
Adult ,Male ,medicine.medical_specialty ,Ureteral Calculi ,Urinary system ,Renal function ,urologic and male genital diseases ,chemistry.chemical_compound ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Electrical and Electronic Engineering ,Instrumentation ,Pelvis ,Ultrasonography ,Creatinine ,Radiation ,medicine.diagnostic_test ,business.industry ,Ceftriaxone ,Ultrasound ,Acute Kidney Injury ,Condensed Matter Physics ,Endoscopy ,Radiographic Image Enhancement ,medicine.anatomical_structure ,chemistry ,Radiographic Image Interpretation, Computer-Assisted ,Abdomen ,Female ,Anuria ,Radiology ,medicine.symptom ,Tomography, X-Ray Computed ,business ,Ureteral Obstruction - Abstract
Noncontrast computed tomography (CT) has great advantage with higher sensitivity and more clear modalities in detecting urinary tract radiolucent calculi in patients with acute renal insufficiency (ARI) compared to other image diagnosis approaches. We report two cases (female, 28 years old; male, 39 years old) with persistent flank pain and acute anuria after the administration of ceftriaxone (4.0 g daily) for 2 days intravenously. No abnormality was found in the kidney-ureter- bladder (KUB) areas with plain abdomen X-rays. A diagnosis of bilateral hydronephrosis was made by ultrasound examination in both cases. Serum creatinine levels reached up to 257 and 810 μ mol/L (normal serum creatinine level is 40-130 μ mol/L), respectively. Vague density spots were noticed in the pelvis with noncontrast multidetector-row CT (MDCT) scanning. However, distinguishable clusters of high-density shadows were seen in pelvic areas with maximum intensity projections (MIP, CT values in 30-128 HU). Ceftriaxone crystal calculi were found on both sides of distal ureters under endoscopy. Renal function recovered in both patients after double-J ureteral stents were installed. Out results demonstrated that noncontrast MDCT scanning and MIP reconstruction as an effective diagnostic tool could provide clear images in detection of radiolucent calculi in urinary tract when conventional X-rays image are not suitable in the patients with obstructive anuria and ARI of unknown origin.
- Published
- 2012
19. Study on the DNA-protein crosslinks induced by chromium (VI) in SPC-A1
- Author
-
Hao You, Jianjun Ding, Xiongbing Lu, and Yanqun Liu
- Subjects
0301 basic medicine ,High concentration ,animal structures ,Chemistry ,Precipitation (chemistry) ,fungi ,030106 microbiology ,Protein dna ,chemistry.chemical_element ,In vitro ,03 medical and health sciences ,chemistry.chemical_compound ,Chromium ,030104 developmental biology ,Hexavalent chromium ,Volume concentration ,Nuclear chemistry - Abstract
Objective: This study was designed to investigate the effect of chromium (VI) on DNA-protein crosslinks (DPC) of SPC-A1 cells. Methods: We exposed SPC-A1 cells were cultured in 1640 medium and treated with the SPC-A1 cells in vitro to different concentrations of Hexavalent chromium Cr(VI) for 2h, the KC1-SDS precipitation assay were used to measure the DNA-protein cross-linking effect. Results: All the different concentrations of Cr(VI) could cause the increase of DPC coefficient in SPC-A1 cells. But this effect was not significant (P>0.05) at low concentrations; while in high concentration Cr(VI) induced SPC-A1 cells could produce DNA-protein cross-linking effect significantly (P
- Published
- 2018
20. Analysis of repeated renal arteriography after percutaneous nephrolithotomy
- Author
-
Zhaohui, He, primary, Hanqi, Lei, additional, Xiongbing, Lu, additional, Caixia, Zhang, additional, Shawpong, Wan, additional, and Guohua, Zeng, additional
- Published
- 2016
- Full Text
- View/download PDF
21. Urine-Derived Stem Cells for Tissue Repair in the Genitourinary System
- Author
-
Yuanyuan Zhang, Xiongbing Lu, Ting Long, Danian Qin, Rongpei Wu, and Junhong Deng
- Subjects
Pathology ,medicine.medical_specialty ,Cell type ,Cell growth ,Angiogenesis ,Transdifferentiation ,Cell ,Biology ,Stem cell marker ,medicine.anatomical_structure ,Cancer research ,medicine ,Stem cell ,Adult stem cell - Abstract
Tissue-specific stem cells can more efficiently repair the tissue from which they originate, compared to other types of adult stem cells. Human urine-derived stem cells (USCs), a subpopulation of cells constantly exposed to urine, possess biological characteristics of stem cells, and thus are an optimal cell source for tissue repair in the genitourinary system. USCs display clonogenicity, high proliferation capacity, stem cell marker expression profiles, and multipotent differentiation capacity. These cells can more efficiently differentiate into the cell types of the genitourinary tract, particular in urothelial cells, renal podocytes, and endothelial cells, compared to other adult stem cells. In addition, USCs secrete numerous growth factors and cytokines to support cell growth, differentiation, resident stem cell recruitment, and immunomodulatory properties. In vivo studies in rodent models of diabetic erectile dysfunction, urinary tract incontinence, and renal dysfunction demonstrated that USCs significantly enhanced organ function via angiogenesis and transdifferentiation, and showed anti-inflammatory and anti-fibrotic properties after implantation of USCs. USCs can be obtained via a simple, non-invasive, and low-cost approach. Thus, USCs offer an extremely promising cell source for cell-based therapy in androurology.
- Published
- 2015
22. Lethal Effect of T Cells Activated by Dendritic Cells with MUC1 Gene Transfection on BIU-87 Cells in vitro
- Author
-
Ye Zhangqun, Siwei Zhou, Gang Luo, Kai Sun, Xiongbing Lu, and Zheng-guo Cao
- Subjects
CD40 ,biology ,Dendritic cell ,Transfection ,digestive system ,Molecular biology ,biological factors ,digestive system diseases ,Interleukin 21 ,Oncology ,Interleukin 12 ,biology.protein ,Cytotoxic T cell ,skin and connective tissue diseases ,Antigen-presenting cell ,neoplasms ,Interleukin 3 - Abstract
Objective: To observe the lethal effect of T cells activated by dendritic cells (DCs) with MUC1 gene transfection (MUC1-DCs) on BIU-87 cells in vitro, and to evaluate the possibility of dendritic cells transfected by MUC1 gene as a vaccine for bladder cancer immunotherapy. Methods: MUC1 was successfully transfected into cultured human blood-derived dendritic cell with lipofectin. The transfection efficiency and immunocompetence were detected by flow cytometry and MTT colourmetry. T lymphocytes activated by MUC1-DCs were used to kill BIU-87 cell lines and normal bladder epithelium in vitro and the killing rate was evaluated by MTT colourmetry. Results: There was significant lethal effect on the BIU-87 cells and normal bladder epithelium with T cells activated by MUC1-transfected DCs, but the lethal effect on the BIU-87 cells significantly exceeded that on normal bladder epithelium (P
- Published
- 2005
23. Urethral Tissue Engineering Using Urine-Derived Stem Cells
- Author
-
Xiongbing Lu, Yuanyuan Zhang, and Antony Devasia
- Subjects
Pathology ,medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Regeneration (biology) ,Mesenchymal stem cell ,Cell therapy ,medicine.anatomical_structure ,Tissue engineering ,Biopsy ,medicine ,Pericyte ,Urothelium ,Stem cell ,business - Abstract
As a tubularized organ in the distal portion of urinary tract system, urethras may have strictures often due to injury, infections, and congenital defects. Two types of tissue engineering technologies have been used to build urethral tissue, namely cell-seeded and non-seeded. Cell-seeded technology uses scaffolds that are seeded in vitro with primary cultured cells obtained from a bladder or oral mucosa biopsy. This composite graft is then implanted back in the host to continue the regenerative process. The non-seeded technology uses cell-free biodegradable scaffolds to allow the natural process of regeneration to occur in vivo and was successfully applied experimentally in partial (patch tissue replacement) urethral reconstruction models. Increasing evidence has demonstrated that use of autologous cells seeded on biodegradable scaffolds achieved better outcomes in urethral tissue regeneration, particularly in long segment of urethral replacement. Stem cells obtained from patients’ own tissues provide an alternative for cell therapy and tissue engineering in urology. Our recent study displayed that a subpopulation of cells isolated from naturally voided urine possesses the features of progenitor/stem cells and has potential as an alternative cell source for tissue engineering and cell therapy in urology. These cells adhere to plastic, are clonogenic, express mesenchymal stem cells/pericyte cell surface markers, and display high telomerase activity. In addition, they possess high expansion capability and multipotential differentiation capacity. Importantly, these cells can give rise to urothelium, smooth muscle to form urethral tissue in vivo in a highly efficient manner when seeded on porous natural collagen-based scaffolds. In this chapter, we discuss the recent progress made in urethral tissue engineering with urine-derived stem cells and acellular matrix for potential use in clinical applications.
- Published
- 2012
24. Ectopic insertion of a duplicated ureter into prostatic urethra: Demonstration by 3D multi-detector computed tomography urography.
- Author
-
Jun Deng, Xiongbing Lu, and Ying Liu
- Subjects
- *
URINARY organ radiography , *ECTOPIC hormones , *GENITOURINARY disease diagnosis , *COMPUTED tomography , *ANGIOGRAPHY complications - Abstract
Ectopic insertion of the ureter in the genitourinary tract is a rare congenital disorder, usually associated with ureteral duplication. Identification of the insertion open is critical for ureteric re-implantation. However, the challenge in the diagnosis of ectopic insertion of the ureter usually is to identify its insertion, particularly when the affected ureter is not dilated. Multi-detector computed tomography (MDCT) urography with nonionic iodinated contrast media delineates the ureteric course in the normal functioning kidney in the excretory phase [1]. This report presented a young male patient with ectopic insertion of a duplicated ureter diagnosed by MDCT urography. Three-dimensional (3D) analysis technology, such as volume rendering (with a color display improving the visualization of complex anatomy and 3D relationships) and maximum intensity projection (similar in principle to projection angiography), is useful for the illustration of urinary tract anatomy [1]. Rotated volume rendering reconstruction images and continual thinner maximum intensity projection reformatted images can be viewed as videos, which provides detail delineation of the ectopic ureteral insertion and its associated ureteral duplication. In this study, we reported MDCT urography and 3D analysis technology as an appropriate diagnostic method for the ectopic ureteral insertion and its associated complications. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
25. Additional file 2: of Urothelium with barrier function differentiated from human urine-derived stem cells for potential use in urinary tract reconstruction
- Author
-
Wan, Qian, Xiong, Geng, Guihua Liu, Shupe, Thomas, Guanghui Wei, Deying Zhang, Liang, Dan, Xiongbing Lu, Atala, Anthony, and Yuanyuan Zhang
- Subjects
3. Good health - Abstract
Table S2. Antibodies used in this study. (DOCX 14Â kb)
26. Additional file 2: of Urothelium with barrier function differentiated from human urine-derived stem cells for potential use in urinary tract reconstruction
- Author
-
Wan, Qian, Xiong, Geng, Guihua Liu, Shupe, Thomas, Guanghui Wei, Deying Zhang, Liang, Dan, Xiongbing Lu, Atala, Anthony, and Yuanyuan Zhang
- Subjects
3. Good health - Abstract
Table S2. Antibodies used in this study. (DOCX 14Â kb)
27. Additional file 3: of Urothelium with barrier function differentiated from human urine-derived stem cells for potential use in urinary tract reconstruction
- Author
-
Wan, Qian, Xiong, Geng, Guihua Liu, Shupe, Thomas, Guanghui Wei, Deying Zhang, Liang, Dan, Xiongbing Lu, Atala, Anthony, and Yuanyuan Zhang
- Subjects
3. Good health - Abstract
Table S3. Percentage of USC expressing urothelial cell markers 2Â weeks after urothelial induction assessed by immunofluorescence. (DOCX 14Â kb)
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.