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Background Hepatocellular carcinoma (HCC) is a common malignant tumor with poor prognosis. Magnetic resonance imaging (MRI) is one of the most effective imaging methods for the early diagnosis of HCC. However, the current MR contrast agents are still facing challenges in the early diagnosis of HCC due to their relatively low sensitivity and biosafety. Thus, the development of effective MR agents is highly needed for the early diagnosis of HCC. Results Herein, we fabricated an HCC-targeted nanocomplexes containing SPIO-loaded mesoporous polydopamine (MPDA@SPIO), sialic acid (SA)-modified polyethyleneimine (SA-PEI), and alpha-fetoprotein regulated ferritin gene (AFP-Fth) which was developed for the early diagnosis of HCC. It was found that the prepared nanocomplexes (MPDA@SPIO/SA-PEI/AFP-Fth) has an excellent biocompatibility towards the liver cells. In vivo and in vivo studies revealed that the transfection of AFP-Fth gene in hepatic cells significantly upregulated the expression level of ferritin, thereby resulting in an enhanced contrast on T2-weighted images via the formed endogenous MR contrast. Conclusions The results suggested that MPDA@SPIO/SA-PEI/AFP-Fth had a superior ability to enhance the MR contrast of T2-weighted images of tumor region than the other preparations, which was due to its HCC-targeted ability and the combined T2 contrast effect of endogenous ferritin and exogenous SPIO. Our study proved that MPDA@SPIO/SA-PEI/AFP-Fth nanocomplexes could be used as an effective MR contrast agent to detect HCC in the early stage.

Activated oncogenes and loss of tumor suppressors contribute to reprogrammed energy metabolism and induce aerobic glycolysis, also known as Warburg effect. MicroRNAs are profoundly implicated in human malignancies by inhibiting translation of multiple mRNA targets. Using hepatocellular carcinoma (HCC) molecular profiles from The Cancer Genome Atlas (TCGA), we identified a handful of dysregulated microRNA in HCC glycolysis, especially miR-34c-3p. Antagonization of miR-34c-3p inhibited the lactate production, glucose consumption, extracellular acidification rate (ECAR), and aggressive proliferation in HCC cells. Hijacking glycolysis by 2-deoxy-d-glucose or galactose largely abrogated the suppressive effects of miR-34c-3p inhibition in HCC. Membrane associated guanylate kinase, WW, and PDZ domain containing 3 (MAGI3) is then identified as a direct functional target of miR-34c-3p in regulating HCC glycolysis and oncogenic activities. Mechanistically, MAGI3 physically interacted with β-catenin to regulate its transcriptional activity and c-Myc expression, which further facilitates the Warburg effect by increasing expression of glycolytic genes including HK2, PFKL, and LDHA. Moreover, overexpressed miR-34c-3p and reduced MAGI3 predicted poor clinical outcome and was closely associated with the maximum standard uptake value (SUVmax) in HCC patients who received preoperative 18 F-FDG PET/CT. Our findings elucidate critical several microRNAs implicated in HCC glycolysis and reveal a novel function of miR-34c-3p/MAGI3 axis in Warburg effect through regulating β-catenin activity.

The single factor variance of viscosity experimental data of blast furnace slag was calculated by Minitab software. The influence of slag basicity and chemical compositions on the slag viscosity was obtained. The results show that there have insignificant effects on the slag viscosity when the slag basicity is over than 1.02, MgO content is less than 11.95% or TiO2 content is less than 12.57%. While the slag basicity can be influenced significantly with the slag basicity less than 1.02, MgO content over than 13.95% and TiO2 content over 13.57%. The Al2O3 content of the slag should be less than 12.75%.

The influence of Al3+ions that had been solid dissolved in C-S-H on the amount of Na+ions adsorbed on C-S-H surface was studied. The experimental results show that the solid dissolution of Al3+ions in C-S-H structure makes the electronegative C-S-H surface increase electronegative amounts, that is the reason why the adsorption ability of C-S-H for Na+ions increase.

The analysis algorithm of parallel factor, combined with fluorescence spectroscopy of three dimensions are used to study the organics identification in petroleum for impurities in surface water and concentration measurement, analysizing the effects on measurement of the coexist of multi-component petroleum pollutants. Treating gasoline, kerosene and diesel as samples, through a mixed solution of a variety of different proportions to imitate the state of coexistence of a variety of petroleum organic, studying PARAFAC in a variety of characteristics of the mixed solution component. Experiment focused on the Steam diesel hybrid solution, Wood kerosene mixed solution and Steam wood kerosene mixed solution, and it can separate their different fluorescence spectrums from the complex mixed composition of three dimensional fluorescence spectrum data matrix. It realized the simultaneous measurement for the main factor in composite samples. And it also calculates the average error rate of concentration .The result indicates that PARAFAC can realize petroleum pollutants composite sample identification and concentration measurement.

According to difference absorption spectrum mixed gas absorption spectrum in technology overlapping problem, a DOAS technology based on the PSO and LS-SVM fusion method was proposed. First, SO2, NO respectively to construct a quantitative analysis model by LS-SVM, And then, using PSO is strong optimization ability of least square support vector machine (SVM) the optimization of the parameters of the algorithm, and then, LS-SVMs parameter are optimized with PSO of the powerful searching ability, Finally, rebuilt the quantitative analysis model. Experiments show that using the improved DOAS method is feasible in solving the mixed gas spectrum overlap problem which has certain practical significance.

The mechanics mechanism of bolt reinforcement of joint fissure test pieces were discussed with the method of variable angle plate indoor model experiment and the deformation of bolt control of jointed slope were analyzed by numerical simulation. The results show that: the bolt reinforcement can improve not only the integrity of joint fissure rock mass, but also be a very good control of deformation of jointed slope. It establishes the foundation on the quantitative for evaluation of joint fissure rock mass deformation and stability.

This thesis discusses the development of Earned Value and introduces the concept of that value and the related indicators. The paper cites real applications and situations in which the method is adopted, analyzes the advantages and disadvantages of method, with the aim to explain its suitability in the management, and to offer suggestions as to the wide use of the method in project management.

The paper regard pillaring agent which is the most important research content of pillared-montmorillonite as subject investigated. By changing the pH value of solution to form different pillaring agent which is used to prepare pillared-montmorillonite. Adsorption capacity of pillared-montmorillonite on SO42-ion as standards, the effection of main process factors—pH value during the preparation of the pillaring agent on adsorption capacity of pillared-montmorillonite is explored.

Al doped ZnO thin films（AZO films） was prepared by sol-gel method. The influence of Parameters of different processes on the crystallization properties, micro-morphology and optical properties of this kind of films were studied, using by X-ray diffractometer, filed emission stereoscan, spectral photometer, hall admeasuring apparatus. The results indicated that the crystallization properties, micro-morphology and optical properties of Al doped ZnO films were best on the condition that the sol density was 0.5mol/L, hat treatment temperature is 600 and there is a 8-layer coating.

As the raw materials of preparing pillared-montmorillonite, before being pillared, Na-montmorillonite is pretreated by adding sodium hexametaphosphate (Na6(PO3)6) to soliquoid of it. Na6(PO3)6 is used as a dispersing agent. It is shown that adsorption capacity of pillared-montmorillonite on SO42-ion is increased compared with non-adding.