Search

Objective To perform a meta-analysis that investigates the prevalence and seroprevalence of celiac disease (CD) in Chinese general population and high-risk populations, and to summarize data from a serological survey of high-risk populations in Guangdong, China. Methods We collected data from the serological survey of high-risk populations in Guangdong, China (n = 1390) by testing serum tissue transglutaminase immunoglobulin A (tTG-IgA), deamidated gliadin peptides immunoglobulin A (DGP-IgA) and deamidated gliadin peptides immunoglobulin G (DGP-IgG), and also searched Medline, PubMed, Embase, Cochrane and 3 Chinese databases up to December 20, 2020 to estimate the pooled prevalence and seroprevalence of CD in China. Results In the serological survey, 0.94% (13/1390) individuals were positive for CD antibodies. In a meta-analysis of 18 studies, the seroprevalence of CD in Chinese general population was 0.27% (95% CI, 0.02-0.71). The seroprevalence of CD in Chinese high-risk populations was 8.34% (95% CI, 4.90-12.54), significantly higher than that in general population (OR 7.27, 95% CI, 4.06-13.04). The prevalence of biopsied-proven CD in high-risk Chinese populations was 4.44% (95% CI, 1.53-8.58). The seroprevalence of CD was varying with geographic origin, higher in Northern China than that in Southern China. Conclusions The basis for diagnosis of CD in Chinese patients should be through early case-finding by serological screening in high-risk groups; generous serological testing in patients with vague symptoms, especially in Northern Chinese.

Intermedin (IMD), a paracrine/autocrine peptide, protects against cardiac fibrosis. However, the underlying mechanism remains poorly understood. Previous study reports that activation of Nucleotide-binding oligomerization domain (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome contributed to cardiac fibrosis. In this study, we aimed to investigate whether IMD mitigates cardiac fibrosis by inhibiting NLRP3. Cardiac fibrosis was induced by angiotensin II (Ang II) infusion for 2 weeks in rats. Western blot, real-time PCR, histological staining, immunofluorescence assay, RNA sequencing, echocardiography and hemodynamics were used to detect the role and the mechanism of IMD in cardiac fibrosis. Ang II infusion resulted in rat cardiac fibrosis, shown as over-deposition of myocardial interstitial collagen and cardiac dysfunction. Importantly, NLRP3 activation and endoplasmic reticulum stress (ERS) was found in Ang II treated rat myocardium. Ang II infusion decreased the expression of IMD and increased the expression of the receptor system of IMD in the fibrotic rat myocardium. IMD treatment attenuated the cardiac fibrosis and improved cardiac function. In addition, IMD inhibited the upregulation of NLRP3 markers and ERS markers induced by Ang II. In vitro, IMD knockdown by small interfering RNA significantly promoted the Ang II-induced cardiac fibroblast and NLRP3 activation. Moreover, silencing of inositol requiring enzyme 1 α (IRE1α) blocked the effects of IMD inhibiting fibroblast and NLRP3 activation. Pre-incubation with PKA pathway inhibitor H89 blocked the effects of IMD on the anti-ERS, anti-NLRP3 and anti-fibrotic response. In conclusion, IMD alleviates cardiac fibrosis by inhibiting NLRP3 inflammasome activation via suppressing IRE1α and cAMP/PKA pathway.

Intermedin (IMD), a paracrine/autocrine peptide, protects against cardiac fibrosis. However, the underlying mechanism remains poorly understood. Previous study reports that activation of nucleotide-binding oligomerization domain (NOD)-like receptor family pyrin domain containing 3 (NLRP3) inflammasome contributes to cardiac fibrosis. In this study, we aimed to investigate whether IMD mitigated cardiac fibrosis by inhibiting NLRP3. Cardiac fibrosis was induced by angiotensin II (Ang II) infusion for 2 weeks in rats. Western blot, real-time PCR, histological staining, immunofluorescence assay, RNA sequencing, echocardiography, and hemodynamics were used to detect the role and the mechanism of IMD in cardiac fibrosis. Ang II infusion resulted in rat cardiac fibrosis, shown as over-deposition of myocardial interstitial collagen and cardiac dysfunction. Importantly, NLRP3 activation and endoplasmic reticulum stress (ERS) were found in Ang II-treated rat myocardium. Ang II infusion decreased the expression of IMD and increased the expression of the receptor system of IMD in the fibrotic rat myocardium. IMD treatment attenuated the cardiac fibrosis and improved cardiac function. In addition, IMD inhibited the upregulation of NLRP3 markers and ERS markers induced by Ang II. In vitro, IMD knockdown by small interfering RNA significantly promoted the Ang II-induced cardiac fibroblast and NLRP3 activation. Moreover, silencing of inositol requiring enzyme 1 α (IRE1α) blocked the effects of IMD inhibiting fibroblast and NLRP3 activation. Pre-incubation with PKA pathway inhibitor H89 blocked the effects of IMD on the anti-ERS, anti-NLRP3, and anti-fibrotic response. In conclusion, IMD alleviated cardiac fibrosis by inhibiting NLRP3 inflammasome activation through suppressing IRE1α via the cAMP/PKA pathway.

Background: Standard measures that define obesity and related disorders varies widely, this study investigated the relationship between different anthropometric indices of obesity criteria and their correlation to hypertension, diabetes, and dyslipidaemia in a local adult population in China.Methods: The study participants underwent the same questionnaire survey, bio-impedance body composition analysis, and blood laboratory test. The t-test and chi-square test were used to compare the characteristics of different groups, and the receiver operating characteristic curve was used to analyse the correlation of different indicators and explore their cut-off values.Results: The study comprised 14,926 participants, of whom 39.80% (5948/14,926) were male, and the mean age of the study population was 56.75±9.74 years. The waist circumference had the greatest influence on all factors, and BMI, AVI, and BRI were similarly correlated. WHtR had the largest AUC for predicting obesity in both sexes, and in addition, we provided a recommended cut-off value of BMI, WHR, WHtR, BAI, OBD, CI, AVI, ABSI and BRI. WHtR had the largest AUC for predicting diabetes, hypertension, and dyslipidaemia, while BMI also served as a good predictive indicator (all P

To understand the effect mechanisms of long-time running and high operation temperature on performance of the direct methanol fuel cell (DMFC) more clearly and directly, in this paper, a new design of 3-electrode system with a solution-type salt bridge has been developed to distinguish the integral polarization into anodic and cathodic polarizations at various temperatures and explore the attenuation mechanism by in situ monitoring the potential of anode during long-time running process at 80 °C, for the first time. The results indicate that the optimized 3-electrode system consists of a standard calomel electrode (SCE) and a solution-type salt bridge placed in the anode hole filled by 0.5 mol L −1 H 2 SO 4 solution. By utilization of the 3-electrode system, the effect mechanisms of the running temperature and time on electrochemical parameters of the DMFC have been found: (1) The increasing operation temperature improves cathodic performance more significantly than that of anode; (2) the attenuation of fuel cell performance mainly comes from that of anode during the 20-h running test at 80 °C, resulting from the sharp drop of electrochemical active surface area of anode. More important, the new 3-electrode system has simplified the detection equipment and reduced the operating difficulty in a practical application for DMFCs, resulting in its portability.

Molecular dynamics (MD) simulations using the reactive force field (ReaxFF) have been performed to elucidate the underlying water-induced disruption mechanism of several prototypical interpenetrated MOFs (IRMOF-9, IRMOF-13, and SUMOF-4). Through the comparison to the corresponding noninterpenetrated MOFs (IRMOF-10 and IRMOF-14), for both the interpenetrated and noninterpenetrated MOFs, structural collapse was always accompanied by the dissociation of the water molecules, with the produced OH– and H+ forming chemical bonds with the Zn2+ ion and O atom of the ligand, respectively. However, the water stability of the interpenetrated MOFs is less than that of the corresponding noninterpenetrated structures. The reasons for the differences between the MOFs in the resistance to water attack are clarified. The water resistance of the noninterpenetrated MOFs is mainly attributed to the strength of the Zn–Oligand, but, the hydrogen bond has little effect. However, a trade-off between the strength of the Zn–Oligand...

Native collagen fibrils (CF) were successfully extracted from bovine tendons using two different methods: modified acid-solubilized extraction for A-CF and pepsin-aided method for P-CF. The yields of A-CF and P-CF were up to 64.91% (&plusmn, 1.07% SD) and 56.78% (&plusmn, 1.22% SD) (dry weight basis), respectively. The analyses of both amino acid composition and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that A-CF and P-CF were type I collagen fibrils. Both A-CF and P-CF retained the intact crystallinity and integrity of type I collagen&rsquo, s natural structure by FTIR spectra, circular dichroism spectroscopy (CD) and X-ray diffraction detection. The aggregation structures of A-CF and P-CF were displayed by UV&ndash, Vis. However, A-CF showed more intact aggregation structure than P-CF. Microstructure and D-periodicities of A-CF and P-CF were observed (SEM and TEM). The diameters of A-CF and P-CF are about 386 and 282 nm, respectively. Although both A-CF and P-CF were theoretically concordant with the Schmitt hypothesis, A-CF was of evener thickness and higher integrity in terms of aggregation structure than P-CF. Modified acid-solubilized method provides a potential non-enzyme alternative to extract native collagen fibrils with uniform thickness and integral aggregation structure.

Aging is an independent risk factor for vascular diseases. Perivascular adipose tissue (PVAT), an active component of the vasculature, contributes to vascular dysfunction during aging. Identification of underlying cell types and their changes during aging may provide meaningful insights regarding the clinical relevance of aging‐related vascular diseases. Here, we take advantage of single‐cell RNA sequence to characterize the resident stromal cells in the PVAT (PVASCs) and identified different clusters between young and aged PVASCs. Bioinformatics analysis revealed decreased endothelial and brown adipogenic differentiation capacities of PVASCs during aging, which contributed to neointimal hyperplasia after perivascular delivery to ligated carotid arteries. Mechanistically, in vitro and in vivo studies both suggested that aging‐induced loss of peroxisome proliferator‐activated receptor‐γ coactivator‐1 α (PGC1α) was a key regulator of decreased brown adipogenic differentiation in senescent PVASCs. We further demonstrated the existence of human PVASCs (hPVASCs) and overexpression of PGC1α improved hPVASC delivery‐induced vascular remodeling. Our finding emphasizes that differentiation capacities of PVASCs alter during aging and loss of PGC1α in aged PVASCs contributes to vascular remodeling via decreased brown adipogenic differentiation.

The adsorption isotherms of two types of hybrid ultra-microporous materials SIFSIX-3-M (M = Fe, Cu, Zn) and M′FSIX-2-Cu-i (M′ = Si, Ti, Ge) for H2, CH4, CO2 and N2 are comparatively studied by grand canonical Monte Carlo simulation (GCMC), and their differences are explained from pore structure and isosteric heat of adsorption. Natural gas and biogas upgrading (CO2/CH4), synthesis gas separation (CO2/H2), flue gas separation (CO2/N2), and steam methane reforming hydrogen production (CH4/H2) are simulated equimolar binary mixture system. The related separation factors are elucidated from the adsorption selectivity and the difference of isosteric heat of adsorption. The microscopic adsorption mechanism of the material is further analyzed by the preferential adsorption sites of the gas on the framework. Our works provide new insights into the adsorption and separation mechanism of HUMs for the energy-related gas, as well as in industrial application.

The Quantum transport of three single metallocene molecule is investigated by performing theoretical calculations using the non-equilibrium Green's function method combined with density functional theory. We find that the three metallocen molecules structure become stretched along the transport direction, the distance between two C p rings longer than the other theory and experiment results. The lager conductance is found in nickelocene molecule, the main transmission channel is the electron coupling between molecule and the electrodes is through the Ni d xz and d yz orbitals and the s , d xz , d yz of gold. This is also confirmed by the highest occupied molecular orbital resonance at Fermi level. In addition, negative differential resistance effect is found in the ferrocene, cobaltocene molecules, this is also closely related with the evolution of the transmission spectrum under applied bias.

Deficiency in α-Klotho is involved in the pathogenesis of vascular calcification. Since intermedin (IMD) 1–53 (a calcitonin/calcitonin gene-related peptide) protects against vascular calcification, we studied whether IMD 1–53 inhibits vascular calcification by upregulating α-Klotho. A rat model of chronic kidney disease (CKD) with vascular calcification induced by the 5/6 nephrectomy plus vitamin D 3 was used for study. The aortas of rats with CKD showed reduced IMD content but an increase of its receptor, calcitonin receptor-like receptor, and its receptor modifier, receptor activity-modifying protein 3. IMD 1–53 treatment reduced vascular calcification. The expression of α-Klotho was greatly decreased in the aortas of rats with CKD but increased in the aortas of IMD 1–53 -treated rats with CKD. In vitro , IMD 1–53 increased α-Klotho protein level in calcified vascular smooth muscle cells. α-Klotho knockdown blocked the inhibitory effect of IMD 1–53 on vascular smooth muscle cell calcification and their transformation into osteoblast-like cells. The effect of IMD 1–53 to upregulate α-Klotho and inhibit vascular smooth muscle cell calcification was abolished by knockdown of its receptor or its modifier protein, or treatment with the protein kinase A inhibitor H89. Thus, IMD 1–53 may attenuate vascular calcification by upregulating α-Klotho via the calcitonin receptor/modifying protein complex and protein kinase A signaling.

A novel 36-metallacrown-6 complex [CuL(N(CN)2)(PF6)]6∙0.5H2O 1 was achieved using a tridendate ligand, 1,4,7-triisopropyl-1,4,7-triazacyclononane (L), and a flexible ligand, dicyanamide in MeOH. The μ1,5 bridging models of the dicyanamide ligand linked the macrocycle to form in a specific size with the chair conformation. The anion was important to form this 36-metallacrown-6 complex, as change was obtained with the larger anion BPh4−, binuclear copper compound 2. The magnetic property indicates that slightly ferromagnetic interactions resulted from a superexchange mechanism. DNA binding properties were also studied. UV and fluorescence spectra showed that complex 1 could bind with DNA.

Four types of 1,3,5,7-tetrakis(4-aminophenyl)adamantine based covalent-organic frameworks (tapa-COFs) have been designed with diamond, ctn and bor net topologies using the methods of molecular mechanics and density function theory. Their low density (0.096–0.258 g cm−3), high porosity (90–96%) and large H2 accessible surface area (5511–6810 m2 g−1) forecast excellent hydrogen uptake capacities. The grand canonical Monte Carlo (GCMC) simulation revealed that at 77 K tapa-COF-1 possesses the highest gravimetric hydrogen storage capacity at 49.10 wt%, while tapa-COF-3 has the highest volumetric hydrogen storage capacity at 58.66 g L−1. Impressively, at 298 K, tapa-COF-1 and tapa-COF-2 possess rather high gravimetric hydrogen uptake capacities, which exceed both the U.S. Department of Energy’s goal (5.5 wt%) for onboard light-duty vehicles for 2020 and the criterion of 6 wt% for commercial use of hydrogen at room temperature. In addition, the possible schemes are also proposed to synthesize the tapa-COFs.

A new development in the palladium-catalyzed allylation reaction of trifluoromethylated ketene aminoacetals was reported. These nucleophilic reagents were prepared by adding aminoalcohols to methyl 3,3,3-trifluoro-2-trifluoromethylpropanoate. The asymmetric version of the allylation reaction was investigated using chiral oxazolidine-based starting materials or chiral palladium catalysts. Asymmetric allylic alkylation was carried out with up to 74% ee using a chiral catalyst. However, no improvement was observed using double stereodifferentiation. These results provided a novel access for trifluoromethylated all-carbon quaternary carbon centers.

Cell-targeted therapies (smart drugs), which selectively control cancer cell progression with limited toxicity to normal cells, have been developed to effectively treat some cancers. However, many cancers such as metastatic prostate cancer (PC) have yet to be treated with current smart drug technology. Here, we describe the thorough preclinical characterization of an RNA aptamer (A9g) that functions as a smart drug for PC by inhibiting the enzymatic activity of prostate-specific membrane antigen (PSMA). Treatment of PC cells with A9g results in reduced cell migration/invasion in culture and metastatic disease in vivo. Importantly, A9g is safe in vivo and is not immunogenic in human cells. Pharmacokinetic and biodistribution studies in mice confirm target specificity and absence of non-specific on/off-target effects. In conclusion, these studies provide new and important insights into the role of PSMA in driving carcinogenesis and demonstrate critical endpoints for the translation of a novel RNA smart drug for advanced stage PC.

A new type of 1,3,5,7-tetraphenyladamantane-based covalent organic framework (adm-COF) was designed under the ctn and bor net topology with the method of molecular mechanics. The computed results reveal that all four designed adm-COFs exhibit extremely high porosity (86–95%) and large H2 accessible surface area (5967–6709 m2 g−1). The grand canonical Monte Carlo method was employed to simulate the adsorption isotherms of H2 gas in these adm-COFs at 77 K and 298 K. The simulated results indicate that, at 77 K and 100 bar, adm-COF-4 has the highest gravimetric H2 adsorption capacity of 38.36 wt%, while adm-COF-1 has the highest volumetric H2 adsorption capacity of 60.71 g L−1. Impressively, the gravimetric H2 adsorption capacity of adm-COF-1 can reach up to 5.81 wt% under 100 bar at room temperature, which is very close to the criterion of 6 wt% for the practical application of hydrogen at room temperature set by the U.S. Department of Energy. In addition, possible schemes for synthesizing these adm-COFs have been proposed.

Leucine-rich repeats and WD repeat domain containing protein 1 (LRWD1) is a testis-specific protein that mainly expressed in the sperm neck where centrosome is located. By using microarray analysis, LRWD1 is identified as a putative gene that involved in spermatogenesis. However, its role in human male germ cell development has not been extensively studied. When checking in the semen of patients with asthenozoospermia, teratozoospermia, and asthenoteratozoospermia, the level of LRWD1 in the sperm neck was significantly reduced with a defective neck or tail. When checking the sub-cellular localization of LRWD1 in the cells, we found that LRWD1 resided in the centrosome and its centrosomal residency was independent of microtubule transportation in NT2/D1, the human testicular embryonic carcinoma, cell line. Depletion of LRWD1 did not induce centrosome re-duplication but inhibited microtubule nucleation. In addition, the G1 arrest were observed in LRWD1 deficient NT2/D1 cells. Upon LRWD1 depletion, the levels of cyclin E, A, and phosphorylated CDK2, were reduced. Overexpression of LRWD1 promoted cell proliferation in NT2/D1, HeLa, and 239T cell lines. In addition, we also observed that autophagy was activated in LRWD1 deficient cells and inhibition of autophagy by chloroquine or bafilomycin A1 promoted cell death when LRWD1 was depleted. Thus, we found a novel function of LRWD1 in controlling microtubule nucleation and cell cycle progression in the human testicular embryonic carcinoma cells. J. Cell. Biochem. 119: 314-326, 2018. © 2017 Wiley Periodicals, Inc.

We have applied the grand canonical Monte Carlo method (GCMC) to in- vestigate the physisorption properties of hydrogen storage in BC3 and carbon nan- otubes, respectively. Some important physical amounts under different temperatures and pressures, such as adsorption isotherms and adsorption amounts were studied. The results show that, the physisorption properties of BC3 nanotube are superior to those of carbon nanotube at all conditions. The main reasons causing such different results between them were analyzed from the interaction energies among nanotubes and H2 molecules. PACS: 61.46.Np, 71.15.Mb, 73.22.-f, 87.15.ak

Grand Canonical Monte Carlo (GCMC) method is employed to simulate the adsorption of methane in several nanoporous zeolites. Adsorption isotherms over the temperature 177-398K and the pressure 0-12MPa are simulated. And their adsorption capacities of methane in these zeolites at different temperatures and pressures are also compared. The results show that: (1) the methane uptake is in the order of LTA>MOR>MFI at the same condition. The isosteric heat can support this conclusion: the value of isosteric heat in LTA is the largest, intermediate in MOR and the least in MFI. (2) The effects of the pore volume, channel size and the energetic interactions between zeolite and methane on adsorption amounts are considered. A large pore volume and a suitable channel size near to the kinetic diameter of a methane molecule are very important for improving the storage capacity of zeolites. Based on this, we conclude that LTA zeolite with a large pore volume and a suitable channel diameter exhibit a most efficient methane storage capacity than MOR and MFI zeolites.

The synthesis, structure and luminescent properties of a cyclometalated iridium (ΙΙΙ) complex are reported. The cyclometalated ligand and auxiliary ligand are 2-(2-fluorophenyl)-benzothiazole (F-BT) and acetylacetone (Hacac), respectively. The complex (F-BT)2Ir(acac) exhibits bright photoluminescence (PL) at room temperature with a high solution PL quantum efficiency of 0.62. Organic electroluminescent (EL) devices are fabricated employing (F-BT)2Ir(acac) as an emissive dopant in 4,4′-N,N′-dicarbazole-biphenyl (CBP) host. EL efficiency shows weak dependence on doping concentration and current density. While the optimized device exhibits a peak efficiency of 28.5 cd/A and a maximum brightness of 49880 cd/m2. An efficiency of 22.7 cd/A at 100 mA/cm2, 80% of the maximum, can be achieved.

An intumescent flame retardant (IFR), pentaerytheritol phosphate ester melamine salt (PPEM) was synthesized and chemically modified by magnesium oxide (MgO) or/and calcium oxide (CaO), respectively. The fire retardant efficiency of MgO and CaO as synergistic agents in polypropylene (PP) was evaluated by normal fire testing methods. The results showed that MgO and CaO exhibited a synergistic effect on fire retardancy of PP. The addition of 0.2% of MgO or CaO into PP/PPEM composite could decrease the self-extinguishing time and improve the limited oxygen index (LOI) obviously. The cone calorimeter (CONE) tests and the thermal analysis revealed that the introduction of MgO and CaO neutralized the acidity of PPEM, which leads to a thermal stabilization and a delay of the intumescent process of the system, and promote the formation of a high quality charred layer which can prevent PP from decomposing. Thus, a suitable amount of MgO and CaO acts as a synergistic agent in the flame-retarded PP/PPEM system. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012

The formation process of goaf was dynamically simulated by similar material simulation esperiment. After mining coal was finished and the goaf was basically stable, deformation regularity of surface migrating was studied under gradual loading in different lacation of goaf . The results show: the residual deformation caused by load arranged on both sides of goaf is bigger than that caused by load arranged in the central of goaf. And the maximum values of residual deformation occur on both sides of goaf. The different mining conditions, load location and size will affect the residual deformation. In single mining conditions, a larger load in the both sides of goaf ,the greater the residual deformation.

Zeolites belong to a most prominent class of nanoporous materials which have been considered as potential sorbents for hydrogen storage. The adsorption of hydrogen molecules on MFI, MOR and LTA zeolites, which encompass a range of different pore structure and chemical composition, has been simulated employing Grand Canonical Monte Carlo (GCMC) method. We compare their capacities of hydrogen storage at different temperatures and pressures. The results show that the adsorbed amounts is in order of LTA>MOR>MFI at the same condition. The effects of pore structure of zeolites, temperature and pressure on the hydrogen adsorption has been examined. The results clearly show that: (1) the temperature effect on the adsorption decreases with decreasing in the number of hydrogen molecules adsorbed. (2) A large volume of micropores and a suitable diameter near to the kinetic diameter of a hydrogen molecule are important for improving the hydrogen-storage capacity of zeolites. Based on this, we can conclude that the LTA zeolite with a large pore volume and a suitable channel diameter exhibits a most efficient hydrogen storage capacity than MOR and MFI zeolites.

Natural manganese ore is a kind of arsenic removal mineral. It is low-cost and widely available. In order to enhance its removal efficiency and adsorption quantity of arsenic, this paper adopted TiCl4 to modify natural manganese ore and optimized the conditions of modification. The results showed that the best modification condition was: TiCl4 at a concentration of 10 mg•L-1, dipping time of 18h, pH 3.05, reaction time of 60 min; under these conditions, the removal rates of As (Ⅲ) and As (Ⅴ) respectively reached 94.87% and 99.31%, much higher compared with natural manganese ore (82.95% and 77.93%). The saturated adsorption quantity of As (Ⅲ) and As (Ⅴ) reached 3.48 mg•g-1 and 3.27 mg•g-1, each increasing 1.25 mg•g-1 and 1.21 mg•g-1. The adsorption of As (Ⅲ) by modified manganese ore fits the Freundlich adsorption isotherm, while As (Ⅴ) fits the Langmuir adsorption isotherm best.

The field-emission properties of capped(5, 5) single-walled carbon nanotubes with hydrogen adsorbed on the tip with and without an applied electric field have been investigated using first-principles density-functional theory. It is found that the structure of carbon nanotubes with hydrogen molecules is stable under field-emission conditions. The local density of states at the Fermi level increases with the adsorption of hydrogen molecules. These results elucidate that the field-emission properties of carbon nanotubes can be enhanced by the adsorption of hydrogen molecules, and are consistent with the experimental results.

In the framework of factorization, based on the first-order of isospin violation, we study direct CP violation in the decay of [Formula: see text] including the effect of ρ–ω mixing. The CP violation depends strongly on Cabibbo–Kobayashi–Maskawa (CKM) matrix elements and the effective parameter, Nc. Recently, the experimental data for the branching ratios of [Formula: see text] are accurate and we can give the strong constraint on the range of Nc. We find that the CP violating asymmetry is large and ranges from -82% to -98% via ρ–ω mixing mechanism when the invariant mass of the π+π- pair is in the vicinity of the ω resonance. We also discuss the possibility to observe the predicted CP violating asymmetries at the LHC.

This paper designs the physical model as geological conditions of XinZhi mine 2204 face of Huozhou Bureau of Mines in Shanxi province, it simulates the formation process and distribution of the mining rock fracture using simulation experiment of similar material, and researches the evolvement law of rock’s fracture network in caving zone, fractured zone using fractal geometry theory. The conclusion of the experiment indicates: with the advance of the working surface, the rock fracture develops from bottom to top, the different fracture network forms corresponds to the different push distance of the working surface, the latter network piles up the previous network, mining makes more complex distribution of rock fractures. The amount of overlying strata separation is three-stage separation law of development, including Initiating phase separation, Expansion phase separation and closed phase separation. Critical layer to any point along the separation experienced by the small → large → small and stable process of change. At the same time, the fractal dimension of the mining rock fracture goes through small to large, under the identical mining width, the fractal dimension of fractured zone is caving zone of 80% to 90%, at last, when the mining work is finished, the fractal dimension dropped to a more stable value.

This paper takes geological conditions of the Li—Jun expressway by Kang JiaGou and Tong De coal mine goaf as prototype, using the simulation experiment of similar material, simulates the formation process and distribution of the mined rock fracture and the damage variable of the mined rock by using the simulation experiment of similar material, At the same time, It researches the evolvement law of rock’s fracture network in caving zone, fractured zone over goaf using fractal geometry theory. It analyses the damage variable law in caving zone, fractured zone using the damage mechanical theory, and found the relationship of damage variable and mined width. The conclusion of the experiment indicates: with the advance of the working surface, the fractal dimension of the mined rock crack goes through small → large →small, and stable process of change. In the identical push distance of the working surface, fractal dimension of caving zone is 1.0—1.2 times of fractured zone. With the advance of the working surface, damage variable increases gradually, under the identical mined width, damage variable of caving zone is greater than fracture zone, caving zone is about 1.04—1.11 times of fracture zone. Damage variable of goaf central zone is greater than edge.

The FPS1 gene coding for the Fps1p aquaglyceroporin protein of an industrial strain of Saccharomyces cerevisiae was disrupted by inserting CUP1 gene. Wild-type strain, CE25, could only grow on YPD medium containing less than 0.45% (v/v) acetic acid, while recombinant strain T12 with FPS1 disruption could grow on YPD medium with 0.6% (v/v) acetic acid. Under 0.4% (v/v) acetic acid stress (pH 4.26), ethanol production and cell growth rates of T12 were 1.7 ± 0.1 and 0.061 ± 0.003 g/l h, while those of CE25 were 1.2 ± 0.1 and 0.048 ± 0.003 g/l h, respectively. FPS1 gene disruption in an industrial ethanologenic yeast thus increases cell growth and ethanol yield under acetic acid stress, which suggests the potential utility of FPS1 gene disruption for bioethanol production from renewable resources such as lignocelluloses.

In this study, we examined differences in serum laminin expression in patients with intractable epilepsy. Our results suggest that elevated laminin may contribute to the pathogenesis of intractable epilepsy. ELISA and western blots were used to measure laminin in the serum of 30 intractable epilepsy patients, 46 nonintractable epilepsy patients, and 20 normal subjects. By ELISA, serum laminin levels were greater in intractable epilepsy patients (177.396 +/- 30.602) and nonintractable epilepsy patients (121.915 +/- 35.215) than in normal control subjects (67.474 +/- 7.197); laminin was significantly greater in the intractable epilepsy group than in the nonintractable epilepsy group. In western blots, the optical density ratio of laminin to ss-actin was 0.871 +/- 0.032 for the intractable epilepsy group, 0.686 +/- 0.017 for the nonintractable epilepsy group, and 0.385 +/- 0.024 for the normal control group. The optical density ratios of the intractable and nonintractable epilepsy groups were higher than those for the normal control group, and the intractable epilepsy group was even greater than the nonintractable epilepsy group. Thus, laminin is significantly increased in epilepsy patients, and this increase is more profound in intractable epilepsy patients.

An algebraic method (AM) used to study the full vibrational spectra of diatomic systems, and an analytical formula used to calculate accurate molecular dissociation energies are applied to study the full vibrational spectra and molecular dissociation energies of some electronic states of homonuclear and heteronuclear diatomic molecules and diatomic ions. Studies show that the AM method and the analytical expression are reliable and economical physical methods for studying full vibrational spectra and molecular dissociation energies of diatomic electronic systems theoretically. They are particularly useful for those diatomic systems whose high-lying vibrational energies may not be available.

The general mixed linear model can be written as y=Xβ+Zu+e. In this paper, we mainly deal with two problems. Firstly, the problem of predicting a general linear combination of fixed effects and realized values of random effects in a general mixed linear model is considered and an explicit representation of the best linear unbiased predictor (BLUP) is derived. In addition, we apply the resulting conclusion to several special models and offer an alternative to characterization of BLUP. Secondly, we recall the notion of linear sufficiency and consider it as regards the BLUP problem and characterize it in several different ways. Further, we study the concepts of linear sufficiency, linear minimal sufficiency and linear completeness, and give relations among them. Finally, four concluding remarks are given.

Three carboxylate silver(I) complexes, [Ag(fbc)]n, (1), [Ag2(cpd)]n (2) and [Ag2(idc)]n (3), were prepared and characterized by X‐ray single crystal analysis, where fbcH is 4‐fluorobenzoic acid, cpdH2 is cyclopentane‐1,1‐dicarboxylic acid and idcH2 is iminodiacetic acid. The three coordination polymers show clear Ag–Ag bonds in their structures. The Ag–Ag bond length 2.850 A both in 1 and 2 is much shorter than that (2.89 A) in metal silver, which is the shortest silver–silver interaction reported. The result of cytotoxicity of the three complexes indicates that they have very high crytotoxicity properties to normal cells and carcinoma cells.

Two cobalt(II) complexes, [Co3L4(APYH)2](SO4)2 (1) and [Co3L4(APY)2](ClO4)2 (2), where APY is 2‐aminopyridine and L is N‐salicylaldehyde‐2‐iminopyridine anion, were prepared and characterized by single crystal structure determination. The crystallographic study shows that each of the two complexes consists of a discrete trinuclear cobalt(II) dication, two sulfate anions in (1) or two perchlorate anions in (2). The similar cations in the two complexes are composed of three cobalt(II) atoms, four tridentate Schiff base ligands (L), and two protonated 2‐aminopyridines for (1) or two 2‐aminopyridine molecules for (2). The authors thank the Education Office of Anhui Province, People's Republic of China for research grant no. 2004kj300zd, and the Natural Science Foundation of Hubei Province, People's Republic of China, for research grant no. 2003ABB010.

Three polymeric silver(I) complexes with terephthalate anions as counterions or ligands, [Ag(pren)]2(tp)·2H2O (1), [Ag(en)][Ag(μ2-tp)]·H2O (2), and [Ag2(μ4-tp)(apy)2] (3) (where pren = 1, 2-propylenediamine, tp =terephthalate dianion, en = ethylenediamine, and apy = 2-aminopyridine) were synthesized and characterized by X-ray single crystal analysis and infrared spectroscopy. 1 crystallizes in the monoclinic space group P211/c with a = 11.3221(5), b = 7.1522(3), c = 14.8128(5)A, V = 1015.77(7)A3, β = 122.132(2), and Z = 2. 2 crystallizes in the orthorhombic space group Pnma with a = 9.6144(6), b = 11.3465(7), c = 11.4810(7)A, V = 1252.5(1)A3, and Z = 4. 3 crystallizes in the monoclinic space group P21/n with a = 8.2003(5), b = 5.8869(4), c = 18.3769(11)A, β = 92.593(1), V = 886.2(1)A3, and Z = 4. Terephthalate dianions are not coordinated to the metal atoms in 1, but act as a μ2-bridging ligand in 2 and as a μ4-bridging ligand in 3. Synthesen und Kristallstrukturen von drei polymeren Silber(I)-Terephthalat-Komplexen mit organischen N-Donorliganden: [Ag(pren)]2(tp)·2H2O, [Ag(en)][Ag(μ2-tp)]·H2O und [Ag2(μ4-tp)(apy)2] Drei polymere Silber(I)-Komplexe mit Terephthalat-Anionen als Gegenionen oder Liganden, [Ag(pren)]2(tp)·2H2O (1), [Ag(en)][Ag(μ2-tp)]·H2O (2) und [Ag2(μ4-tp)(apy)2] (3) (pren = 1, 2-Propylendiamin, tp = Terephthalat-Dianion, en = Ethylendiamin und apy = 2-Aminopyridin) wurden synthetisiert und mittels Rontgen-Einkristallstrukturanalyse sowie Infrarotspektroskopie charakterisiert. 1 kristallisiert in der monoklinen Raumgruppe P21/c mit a = 11.3221(5), b = 7.1522(3), c = 14.8128(5)A, V = 1015.77(7)A3, β = 122.132(2) und Z = 2. 2 kristallisiert in der orthorhombischen Raumgruppe Pnma mit a = 9.6144(6), b = 11.3465(7), c = 11.4810(7)A, V = 1252.5(1)A3 und Z = 4. 3 kristallisiert in the monoklinen Raumgruppe P21/n mit a = 8.2003(5), b = 5.8869(4), c = 18.3769(11)A, β = 92.593(1), V = 886.2(1)A3 und Z = 4. Die Terephthalat-Dianionen sind in 1 nicht an die Metallatome koordiniert, sind aber μ2-verbruckende Liganden in 2 und μ4-verbruckende Liganden in 3.

The reaction of Ag2O and carboxylate ligands in ammonium solution results in three coordination polymers, [Ag(fbc)]n 1, [Ag2(cpd)]n 2 and [Ag2(idc)]n 3, where fbcH is 4-fluorobenzoic acid, cpdH2 is cyclopentane-1,1-dicarboxylic acid and idcH2 is iminodiacetic acid. The X-ray crystal structural analysis indicates that compounds 1 and 2 are two-dimensional frameworks and 3 is a three-dimensional framework. All the three complexes show clear Ag–Ag bonds and high cytotoxicity properties to normal cells and carcinoma cells.

Coordination compounds of the coinage monovalent ions have received considerable attention in the last three decades. In particular, the study of their biological activity is of importance. In the ...