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Background: To investigate the drug resistance and clinical characteristics of hospitalized children with drug-resistant Mycoplasma pneumoniae pneumonia (MRMP).Methods: Sixty patients with MPP admitted to the Second Pediatric Respiratory Ward of Shengjing Hospital, Affiliated to China Medical University from November 2016 to February 2017 were enrolled in the study.Results: Of these 53/60 (88.3%) patients had Mycoplasma pneumoniae nucleic acid identified by throat swab. 23S rRNA V region gene sequencing was performed, 47/49 (95.9%) had mutation sites, including 46 cases of A2063G, one case of A2064G, two cases of no mutation, and a final drug resistance rate of 95.9%. The summary characteristics of the 47 cases of drug-resistant MPP were based on 22 male and 25 female patients. The onset age was 6.9 ± 2.5 years and the total fever duration was 9.8 ± 3.7 days. The leukocyte count during the acute phase was (8,300 ± 4,200) cells/mm3, C-reactive Protein (CRP) was 18.2 (8.2–32.5) mg/L, neutrophil/lymphocyte ratio (NLR) was 2.1 (1.5–3.3), There was no significant difference between the acute phase and the convalescent phase for leukocyte count, P = 0.336. The NLR and CRP levels were significantly higher during the acute phase compared to the recovery period (P < 0.05). The level of lactate dehydrogenase (LDH) increased in 65.7% of patients, with a median of 248.5 (200.0–299.7) U/L. D-dimer levels were elevated in 59.4% of patients, with a median of 301.0 (188.5–545.0) mg/L. A total of 23/47 (48.9%) patients were diagnosed with severe MPP. The incidence of extra-pulmonary complications was 38.2%. Conclusions: In summary, MRMP patients had a fever of long duration, higher inflammatory index, higher LDH and D-dimer levels, and an increased incidence of extra-pulmonary complications.

Background The point mutations in 23S rRNA gene of Mycoplasma pneumoniae (M. pneumoniae) can lead to high-level resistance to macrolides. This study aimed to evaluate allele-specific real-time PCR (ASPCR) to detect the resistance-related mutations located at positions A2063G and A2064G of 23S rRNA gene. Methods We detected 178 pharyngeal swab specimens and calculated the proportions of resistant and sensitive quasispecies using ASPCR assays. ASPCR assays can detect down to 10 copies of 23S rRNA gene and achieved sensitivities of

The mineralization of 125 mL of 50–300 mg L−1 cefoperazone (CFPZ) has been comparatively studied by electrochemical advanced oxidation processes (EAOPs) like anodic oxidation (AO), electro-Fenton (EF) and photoelectro-Fenton (PEF) with a RuO2/Ti or boron-doped diamond (BDD) anode and an activated carbon fiber (ACF) cathode. A microwave discharge electrodeless lamp (MDEL) was used as the UV source in PEF process. CFPZ decays always followed pseudo-first-order kinetics and their constant rates increased in the order: AO

Separation and treatment of human urine from domestic wastewater have been recognized as a sustainable alternative to conventional urban sanitation system, but the unique water quality of source-separated urine, such as high nutrient, insufficient alkalinity and low COD/nitrogen ratio, poses challenges to the biological nitrogen removal process. It was hypothesized that a physicochemical pre-treatment should be adopted to not only reduce nitrogen load but adjust water quality suitable for biological treatment. In this study, a vacuum thermal stripping process coupled to acid absorption was used as a pre-treatment to recover ammonia from hydrolyzed urine in the form of ammonium sulphate. The maximal ammonia mass transfer coefficient of 17.6 mm/h was obtained under 60 °C and 21.3 kPa 80% of the nitrogen in hydrolyzed urine was recovered in 3 h batch stripping experiments without pH adjustment, corresponding to a nitrogen recovery loading rate of 36 kg N/m3⋅d. The majority of organic matters were retained in urine solutions, thus COD/N and alkalinity/NH4+ N ratios were elevated to 4.75 and 4.37, respectively. Phosphorus recovery could be simultaneously obtained in stripping process by adding MgCl2. The remaining nitrogen was effectively removed via short-cut nitrification and denitrification in an anoxic/oxic membrane bioreactor. More than 95% of overall nutrient recovery/removal from urine was achieved with a minimal consumption of external resources. The economic assessment of the technique showed that the recovery/removal of nutrients from 1 m3 of urine can make a profit of € 0.26.

Solutions of 200 mg L−1 cefalexin (CLX), an antibiotic with high usage frequency and biodegradation resistance, have been comparatively degraded by electro-Fenton (EF) and photoelectro-Fenton (PEF) processes using two kinds of activated carbon fiber (ACF) cathodes with different physical properties. These two ACFs shared similar pore volumes and pore diameters but varied BET surface areas, which were confirmed to be 0.5210 cm3 g−1, 2.26 nm and 921 m2 g−1 for ACF1, while 0.6508 cm3 g−1, 2.16 nm and 1206 m2 g−1 for ACF2, respectively. Their oxidation abilities were comparatively assessed in terms of degradation kinetics and mineralization rates, which increased in the order: ACF1-EF OH accumulation in more reaction sites provided. After 120 min electrolysis, ACF1 exhibited 1510 μM H2O2 and 37 μM OH accumulation, while ACF2 generated 1934 μM H2O2 and 85 μM OH. Moreover, ACF cathode with more developed pore structure also revealed faster formation of degradation by-products like inorganic ions (NH4+ and NO3− ions) and short-chain carboxylic acids (acetic, formic, oxamic and oxalic acids), as well as enhanced removal for partial acids. In order to gain a deeper understanding of degradation mechanisms for ACF2-PEF system, evolutions of six aromatic by-products generated from sulfoxidation, hydroxylation and decarboxylation were confirmed by UPLC-QTOF-MS/MS determination. Based on the above identifications of the degradation intermediates, a plausible reaction pathway for CLX removal was proposed.

Glass antireflection membrane can effectively increase the collection and utilization of sunlight by daylighting equipment, and has been widely used in solar energy related fields. Therefore, exploring the development of high-performance glass antireflection membrane is an essential topic in the current energy utilization research. This paper introduces the general situation of the preparation technology of antireflection membrane internationally, especially the specific method of producing antireflection membrane of silica by sol-gel method in the research. According to the analysis of the results, the significance of membrane surfactant which is similar to Triton X-100 was obtained, and the development prospect of this technology was discussed.

Oxidation of a commonly-used β-lactam pharmaceutical, cefoperazone (CFPZ), was systematically investigated by anodic oxidation (AO), AO in presence of H2O2 electro-generation (AO-H2O2) and electro-Fenton (EF) processes with an activated carbon fiber cathode from the biodegradability viewpoint. The degradation and mineralization rates increased in a sequence of AO

Per- and polyfluoroalkyl substances (PFASs) have been detected in many agricultural products in contaminated fields and in supply chains. Roots are the main organ in plants to uptake and bio-accumulate PFASs, but the changes of metabolic regulation in roots by PFASs are largely unexplored. Here, lettuce exposed to perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) at different concentrations (500, 1000, 2000 and 5000 ng/L) was investigated via metabolomics. Many key metabolites, such as antioxidants, lipids, amino acids, fatty acids, carbohydrates, linolenic acid derivatives, purine and nucleosides, were significantly altered. Tyrosine metabolism, purine metabolism, isoquinoline alkaloid biosynthesis and terpenoid backbone biosynthesis were altered in roots by PFOA and PFOS. Tricarboxylic acid cycle was perturbed by 5000 ng/L exposure. Activation of antioxidant defense pathways, reallocation of carbon and nitrogen metabolism, regulation of energy metabolism and purine metabolism were reprogrammed in roots. Lettuce employed multiple strategies to increase tolerance to PFOA and PFOS, which includes the adjustment of membrane composition, elevation of inorganic nitrogen fixation and respiration, accumulation of sucrose and regulation of signaling molecules. The results of this study offer insights into the molecular reprogramming of plant roots in response to PFAS exposure and provide important information for the risk assessment of PFASs in environment.

In this study, a novel photoelectro-Fenton (PEF) process using microwave discharge electrodeless lamp (MDEL) as a UV irradiation source was developed for the removal of antibiotic ciprofloxacin (CIP) in water. Comparative degradation of 200mgL-1 CIP was studied by direct MDEL photolysis, anodic oxidation (AO), AO in presence of electrogenerated H2O2 (AO-H2O2), AO-H2O2 under MDEL irradiation (MDEL-AO-H2O2), electro-Fenton (EF) and MDEL-PEF processes. Higher oxidation power was found in the sequence: MDEL photolysis < AO < AO-H2O2< MDEL-AO-H2O2< EF < MDEL-PEF. Effects of current density, pH, initial Fe2+ concentration and initial CIP concentration on TOC removal in MDEL-PEF process were examined, and the optimal conditions were ascertained. The releases of three inorganic ions (F-, NH4+ and NO3-) and two carboxylic acids (oxalic and formic acids) were qualified. Seven aromatic intermediates mainly generated from hydroxylation, dealkylation and defluorination of CIP were detected by UPLC-QTOF-MS/MS technology. Therefore, plausible degradation sequences for CIP degradation in MDEL-PEF process including all detected products were proposed.

This work investigated the effect of the chelation of Fe 3+ with a fluoroquinolone (FQ) antibiotic ciprofloxacin (CIP) on the catalytic behavior of Fe 3+ /Fe 2+ and the degradation and mineralization of CIP in electro-Fenton (EF) process using activated carbon fiber (ACF) felt cathode. First of all, effect of initial Fe 2+ and Fe 3+ concentrations on the degradation and mineralization of CIP in EF process was compared, while the optimal initial Fe 2+ and Fe 3+ contents were both verified to be 1.50 mmol dm −3 , in which 73% and 72% of initial TOC were eliminated after 360 min electrolysis, respectively. Secondly, Fe 3+ -CIP chelate was produced during CIP degradation in EF process, whose predominant form was confirmed as 1:2 metal-ligand stoichiometry. The formation of Fe 3+ -CIP chelate had no obvious inhibition on the degradation and mineralization of CIP due to the efficient Fe 3+ reduction on the ACF cathode with excellent catalytic feature. About 17% and 35% of initial 1.50 mmol dm −3 Fe 3+ were reduced to Fe 2+ at 18 mA cm −2 in ultrapure water and 200 mg dm −3 CIP solutions during 30 min pre-aeration of EF process, respectively, while higher Fe 2+ concentration was maintained for initial 1.50 mmol dm −3 Fe 3+ during CIP degradation. Finally, the evolution of F − , NH 4 + and NO 3 − ions released to the acidic medium were determined, as well as the formation of short-chain carboxylic acid (oxalic, oxamic and formic acids). Eight aromatic intermediates were identified using UPLC-QTOF-MS/MS, and a feasible mineralization pathway was proposed.

Source separation and treatment of human urine have been recognized as a resource-efficient alternative to conventional urban drainage, not only reducing nutrient loads on municipal wastewater treatment plants, but recovering valuable resources from waste streams. In this work, on-site phosphorus (P) recovery from real urine was carried out by using the brine from a reverse osmosis process as the flush water for urine-diverting toilets and a P precipitant, while nitrogen (N) was removed via short-cut nitrification-denitrification (SCND) in a membrane bioreactor (MBR). More than 90% of P was recovered by mixing the urine with reverse osmosis brine (1:1, v/v) under the condition of pH > 9.0. The recovered precipitates contained 10–15% of P and can potentially be reused for phosphate fertilizer production. Stable SCND was achieved in a MBR, and 45% of N was removed with the organic compounds in urine as the electron donor for denitrification. Methanol addition significantly elevated denitrification, which in turn replenished the alkalinity required for nitrification. More than 99% of P, 90% of organics and 90% of N were removed in the combined precipitation and MBR process. Nitrosomonas was observed to be the predominant ammonium-oxidizing bacteria, while nitrite-oxidizing bacteria (NOB) were absent in the microbial communities as revealed by fluorescence in situ hybridization and pyrosequencing technique. High concentrations of free ammonia and nitrite acids, as well as low dissolved oxygen, are the prevailing factors to inhibit the growth of NOB, which allows for stable operation of SCND in the MBR.

Phosphorus (P) recovery from waste streams has recently been recognized as a key step in the sustainable supply of this indispensable and non-renewable resource. The feasibility of using brine from a reverse osmosis (RO) membrane unit treating cooling water as a precipitant for P recovery from source separated urine was evaluated in the present study. P removal efficiency, process parameters and precipitate properties were investigated in batch and continuous flow experiments. More than 90% of P removal was obtained from both undiluted fresh and hydrolyzed urines by mixing with RO brine (1:1, v/v) at a pH over 9.0. Around 2.58 and 1.24 Kg of precipitates could be recovered from 1 m3 hydrolyzed and fresh urine, respectively, and the precipitated solids contain 8.1–19.0% of P, 10.3–15.2% of Ca, 3.7–5.0% of Mg and 0.1–3.5% of ammonium nitrogen. Satisfactory P removal performance was also achieved in a continuous flow precipitation reactor with a hydraulic retention time of 3–6 h. RO brine could be considered as urinal and toilet flush water despite of a marginally higher precipitation tendency than tap water. This study provides a widely available, low - cost and efficient precipitant for P recovery in urban areas, which will make P recovery from urine more economically attractive.

In this study, a novel self-sustainable solar assisted photoelectro-Fenton (SPEF) system driven by a solar photovoltaic cell was developed for the efficient mineralization of antibiotic trimethoprim (TMP) in water. A comparative degradation of 200mgL(-1) TMP by RuO2/Ti anodic oxidation (AO), anodic oxidation with H2O2 electrogeneration (AO-H2O2), electro-Fenton (EF) and SPEF was investigated. SPEF was proved to exhibit the highest oxidation power, i.e., more than 80% TOC was removed after 360min SPEF treatment of 200mgL(-1) of TMP under optimal conditions at pH 3.0, 1.0mM Fe(2+) and 18mAcm(-2). Influences of current density, pH, initial Fe(2+) and initial TMP concentration on SPEF process were also studied. Ten aromatic intermediates generated from hydroxylation, carbonylation and demethylation reactions were identified using UPLC-QTOF-MS/MS system during the SPEF treatment, together with three carboxylic acids (oxamic, oxalic and formic acids) and two inorganic ions (NH4(+) and NO3(-)) measured. Therefore, a reasonable pathway of TMP degradation in SPEF process was proposed.

This study aims to evaluate laboratory methods for the diagnosis of Mycoplasma pneumoniae . Ninety-three children infected with Mycoplasma pneumoniae were tested, and large sample size makes the results representative. The pharyngeal swabs and serum samples were mostly collected from the first week of the disease, or from the second to third week of the disease in recovery children. The pharyngeal swabs from healthy children were cultured and two of them were positive for the bacterium. The accuracy of laboratory diagnostic methods for Mycoplasma pneumoniae were evaluated against serum test with 4-fold increase or decrease in the titers of antibody in two serum samples. The specificity of culture method was high, which suggested that the method was suitable for clinical research. In acute phase, the sensitivities of single serological test or simultaneous amplification and testing of Mycoplasma pneumoniae (SAT) were 71.7% and 85%, respectively.

The aim of this study was to evaluate the inhibitory effect of antibiotics and Xiao’er Feire Kechuan Oral Solution on Mycoplasma pneumoniae (MP) clinical isolates. Twenty clinical isolates containing A-to-G transition at position 2063 and 10 clinical isolates without mutations in 23S rRNA V regions were randomly selected. The international standard strain FH was chosen as control strain. The minimum inhibitory concentration (MIC) of macrolide, quinolones, tetracycline, and Xiao’er Feire Kechuan Oral Solution to MP clinical isolates were performed using broth microdilution method. In vitro antibiotic susceptibility test of MP clinical isolates showed that MP showed high resistance to macrolide antibiotics (erythromycin and azithromycin); MIC of both were more than 64 μg/mL. The MICs of erythromycin and azithromycin for clinical isolates without mutations in 23S rRNA V regions were ≤0.5 μg/mL. The MICs of tetracycline and levofloxacin for all clinical isolated strains were ≤2.0 μg/mL and ≤1.0 μg/mL, respectively. The MIC of Xiao’er Feire Kechuan Oral Solution was 13.828∼6.914 mg/mL. In vitro, the drug resistance of MP to macrolide antibiotics is higher, MP clinical isolates are sensitive to tetracycline and levofloxacin, and Xiao’er Feire Kechuan Oral Solution also has a certain inhibitory effect on the macrolide-resistant MP.

Phosphorus (P) recovery from wastewater has been recognized as a critical technology for solving the sustainable supply of this indispensable and non - renewable natural resource. In this study, the cost - free magnesium and calcium sources of using the cooling water system effluent (CWSE) in two thermal power plants were proposed (Z - CWSE and G - CWSE) and the P recovery performance from source - separated urine was investigated. About 90% P recovery efficiency was achieved from the hydrolyzed urine when Z - CWSE and G - CWSE were added at the Ca: Mg: P molar ratios of 3.1 : 4.0: 1 and 3.6 : 3.4: 1, respectively. More than 95% P recovery performance was obtained from the fresh urine as the initial pH of the CWSE - FU mixtures was adjusted to over 9.5 and 10.0, respectively. The precipitates obtained contain 10.84–17.04% Ca, 6.22–9.58% P, 0.75–3.76% Mg and 0.13–0.23% N. XRD analysis confirmed the presence of struvite in the precipitates. The reuse of precipitates is secure due to extremely low contents of heavy metals. The feasibility of using CWSEs as the flushing water in urinals and toilets was assessed. Besides, we proposed CWSEs could be invoked as precipitants in various wastewaters as long as it contains considerable phosphate, e.g. P concentration more than 100 mg/L and 50 mg/L for Z - CWSE and G - CWSE, respectively.

The mineralization of 125 mL of 50-300 mg L

The occurrence of perfluorinated compounds (PFCs) in vegetables and fruits, as well as agricultural environment, was investigated in the downstream regions of Changshu fluorine-chemical industrial park (CFCIP) in China. Twenty-one PFCs were analyzed in irrigation water, agricultural soil, typical vegetables, and fruits, with the maximum total PFC concentrations of 369.9 ng/L, 64.7 ng/g dw, 11.5 ng/g ww, and 10.5 ng/g ww, respectively. Short-chained perfluoroalkyl carboxylic acids (PFCAs) such as perfluorooctanoic acid (PFOA), perfluorobutanoic acid (PFBA), and perfluorohexanoic acid were the dominant PFCs in terms of their concentrations and detection frequency. PFCs in irrigation water and agricultural soils showed a decreasing trend with increasing distance from CFCIP, while this pattern was not observed in agricultural products. The predominant compounds varied in different vegetables and fruits. Simultaneous bioaccumulation of PFBA and PFOA was found in melons and solanaceous species and pears. Leafy vegetables and grapes exhibited high bioaccumulation of PFOA and PFBA, respectively. Health risk assessment by calculating estimated daily intake showed that no direct risk was caused by the consumption of vegetables and fruits for the residents in the investigated regions. However, the tolerable weekly intake of PFOA exceeded the established thresholds for the adult residents. A comprehensive health assessment of the dietary exposure of PFCs, including all exposure pathways, in fluorine-chemical industrial park-impacted regions is needed.

Solutions of 500 mL 200 mg L(-1) fluoroquinolone antibiotic levofloxacin (LEVO) have been degraded by anodic oxidation (AO), AO with electrogenerated H2O2 (AO-H2O2) and electro-Fenton (EF) processes using an activated carbon fiber (ACF) felt cathode from the point view of not only LEVO disappearance and mineralization, but also biodegradability enhancement. The LEVO decay by EF process followed a pseudo-first-order reaction with an apparent rate constant of 2.37×10(-2)min(-1), which is much higher than that of AO or AO-H2O2 processes. The LEVO mineralization also evidences the order EF>AO-H2O2>AO. The biodegradability (BOD5/COD) increased from 0 initially to 0.24, 0.09, and 0.03 for EF, AO-H2O2 and AO processes after 360 min treatment, respectively. Effects of several parameters such as current density, initial pH and Fe(2+) concentration on the EF degradation have also been examined. Three carboxylic acids including oxalic, formic and acetic acid were detected, as well as the released inorganic ions NH4(+), NO3(-) and F(-). At last, an ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry was used to identify about eight aromatic intermediates formed in 60 min of EF treatment, and a plausible mineralization pathway for LEVO by EF treatment was proposed.

Removal of the refractory organic matters in leachate brines generated from nanofiltration unit in two full-scale municipal solid waste landfill leachate treatment plants was investigated by Fenton oxidative-coagulation and ultraviolet photo - Fenton processes in this study. Fenton oxidative-coagulation was performed under the condition of an initial pH of 5.0 and low H2O2/Fe(2+) ratios. After precipitate separation, the remaining organic constituents were further oxidized by photo - Fenton process. For both leachate brines with varying pollution strength, more than 90% COD and TOC reductions were achieved at H2O2/Fe(2+) dosages of 35 mM/8 mM and 90 mM/10 mM, respectively. The effluent COD ranged 120-160 mg/L under the optimal operating conditions, and the biodegradability was increased significantly. Fenton oxidative-coagulation was demonstrated to contribute nearly 70% overall removal of organic matters. In the combined processes, the efficiency of hydrogen peroxide varied from 216 to 228%, which may significantly reduce the operating cost of conventional Fenton method. Six phthalic acid esters and thirteen polycyclic aromatic hydrocarbons were found in leachate brines, and, on the average, around 80% phthalic acid esters and 90% polycyclic aromatic hydrocarbons were removed by the combined treatments.

Poly- and perfluoroalkyl substances (PFASs) are becoming common pollutants in natural environment, while the toxic effects and defense mechanisms in agricultural plants are poorly understood. Here, lettuce exposed to either perfluorooctanoic acid (PFOA) or perfluorooctane sulfonate (PFOS) at two different concentrations (500, 5000 ng/L) in hydroponic media was investigated via metabolomics. Under the tested conditions, the growth and biomass of lettuce were not affected by PFOA and PFOS, but metabolic profiles in leaves were altered. The composition and metabolism of lipids, carbohydrates, fatty acids, amino acids and some antioxidants were regulated, compromising the nutritional quality of the plants. Key pathways in energy metabolism were disturbed by both PFOA and PFOS, including tricarboxylic acid cycle, glyoxylate and dicarboxylate metabolism and pyruvate metabolism. Amino acid metabolism, e.g., phenylalanine and tyrosine, was disturbed by PFOA. The metabolism of linoleic acid was disturbed by PFOS. The changes of antioxidants and 8-hydroxy-deoxyguanosine indicated the occurrence of oxidative stress and DNA damage under PFOA or PFOS exposure. The main defense processes against PFASs exposure in lettuce included alteration in plasma membrane, activation of antioxidant systems, increase of tolerance and repair of DNA injury. These findings help elucidate the response of plants to PFASs in a molecular-scale perspective.

Graphical abstract Dogs are often feed with internal organs of livestock in Tibetan communities, where home slaughtering is still popular., Highlights • A community based cross-sectional study of CE was conducted in Tibetan communities. • This is the first knowledge, attitude, and practice (KAP) study of CE in the areas. • Most of residents had positive attitude toward treatments of the disease. • Their practice about prevention and control of the disease was not so good. • Feeding dogs with offal is still one of risk factors of CE transmission., Cystic echinococcosis (CE) is a global parasitic zoonosis caused by Echinococcus granulosus. The disease is highly endemic in western China, especially in Tibetan areas, because of poor economic development and hygiene conditions, limited community knowledge of CE, a large scale of dogs, and home slaughtering of livestock. Although many researchers have analyzed risk factors of CE transmission in Tibetan Plateau, there are rare reports of knowledge, attitude, and practice (KAP) of residents about CE in Tibetan communities. In our current study, community based cross-sectional study was conducted in three townships in Xiahe County, Gannan Tibetan Autonomous Prefectures of Gansu Province from May to September 2013. A total of 972 participants originating from Tibetan communities of 31 villages in the 3 townships were registered and data were collected using structured questionnaires. From the total of 972 study participants (457 males and 515 females), 65.9% heard of the disease CE. Most of them (96.1%) would like to accept CE inspection. About half of the peoples feed their dogs often and major of them do not play with the dogs. Risk factors included resident, knowing dog could be infected, knowing eating could be route of infection, oldest dog's age, usually feed your dog by self, feed dogs with internal organs. In general our findings showed that most of residents had positive attitude toward treatments of the disease, but their practice about disease prevention and control was low. Therefore, our study called for continued and strengthened education of changing the life style, especially the behaviors related to dogs.

The fate and transport of pathogenic bacteria from wastewater treatment facilities in the Earth's subsurface have attracted extensive concern over recent decades, while the impact of treated-wastewater chemistry on bacterial viability and transport behavior remains unclear. The influence of retention time in effluent from a full-scale municipal wastewater treatment plant on the survival and deposition of Staphylococcus aureus and Escherichia coli strains in sand columns was investigated in this paper. In comparison to the bacteria cultivated in nutrient-rich growth media, retention in treated wastewater significantly reduced the viability of all strains. Bacterial surface properties, e.g., zeta potential, hydrophobicity, and surface charges, varied dramatically in treated wastewater, though no universal trend was found for different strains. Retention in treated wastewater effluent resulted in changes in bacterial deposition in sand columns. Longer retention periods in treated wastewater decreased bacterial deposition rates for the strains evaluated and elevated the transport potential in sand columns. We suggest that the wastewater quality should be taken into account in estimating the fate of pathogenic bacteria discharged from wastewater treatment facilities and the risks they pose in the aquatic environment.

Biologically treated leachate usually contains considerable amount of refractory organics and trace concentrations of xenobiotic pollutants. Removal of refractory organics from biologically treated landfill leachate by a novel microwave discharge electrodeless lamp (MDEL) assisted Fenton process was investigated in the present study in comparison to conventional Fenton and ultraviolet Fenton processes. Conventional Fenton and ultraviolet Fenton processes could substantially remove up to 70% of the refractory organics in a membrane bioreactor treated leachate. MDEL assisted Fenton process achieved excellent removal performance of the refractory components, and the effluent chemical oxygen demand concentration was lower than 100 mg L−1. Most organic matters were transformed into smaller compounds with molecular weights less than 1000 Da. Ten different polycyclic aromatic hydrocarbons were detected in the biologically treated leachate, most of which were effectively removed by MDEL-Fenton treatment. MDEL-Fenton process provides powerful capability in degradation of refractory and xenobiotic organic pollutants in landfill leachate and could be adopted as a single-stage polishing process for biologically treated landfill leachate to meet the stringent discharge limit.

Additional file 1. Additional table and figure.

High photocatalytic activity La2Ti2O7 was synthesized by a novel mineralization polymerizable complex (MPC) method. Compared with La2Ti2O7 prepared by polymerizable complex (PC) method, MPC-La2Ti2O7 showed larger surface areas, higher crystallization and more uniform morphology. These factors resulted in a dramatic improvement of its photocatalytic activity for hydrogen evolution. The photocatalytic activity of MPC-La2Ti2O7 was about three times higher than that of PC-La2Ti2O7.

Higher education will provide a good platform for the goals of constructing the Resource-economized Society and sustaining economic growth and controlling greenhouse gas emissions. By initiating a new course of Technology and Policy Management of Low-carbon, the course content and the teaching way in local university were studied, and the prevalence of low-carbon policy, CCS technology and low carbon information/knowledge has been promoted. More importantly, the environmental protection awareness of contemporary college students in low-carbon economy and sustainable development have been increased, and it can provide instructions in the professional field engaged in the future. Index Terms - low carbon, free optional course, design of course content, Environmental education of high school, environmental awareness

Taking the Daniel Fong and Yulin Village in Beijing as cases to study. the quality characteristics of rainwater runoff and the pollution situation were obtained by monitoring the rainwater samples from the roof and road in different rainfall intensity. The emphasis was placed on the characteristics of four heavy metals (Zn, Cu, Cr and Cd) over time, as well as the relationship between the Concentration of heavy metals and The concentration of suspended solids. The results showed that compared with the roof runoff, surface runoff water quality was poor. At start, the concentration of heavy metals was larger, with the rainfall time, the concentration decreased. The linear correlations of the Concentration between Zn, Cu, Cr, Cd and SS were different. The correlations of the Concentration between Cd and SS was the highest, with a correlation coefficient of 0.6245, its concentration was most significantly impacted by the Concentration of SS.