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Objective To construct a new type of glucosamine/DNA composite nanostructure (NTGlcN) assembled without magnesium, verify whether or not glucosamine can mediate the assembly of DNA nanotubes (NT) and assess its effect on the function of Raw264.7 cells. Methods Utilizing the gradient annealing method with 3 DNA single strands Y1, Y2, and Y3, glucosamine (GlcN) was employed to mediate the assembly of DNA NT, resulting in the formation of glucosamine/DNA composite nanostructures. Atomic force microscopy (AFM) was used to observe the surface structure of the nanomaterial and dynamic light scattering (DLS) was used to measure its size. RAW264.7 cells were used in cell experiments. The cytotoxicity of GlcN and NTGlcN was assessed using CCK-8 assay. Flow cytometry and laser confocal microscopy were employed to investigate the cellular uptake efficiency of the nanostructures. The effects of NTGlcN and NTMg (Mg2+-assembled of DNA NT) on the expression levels of inflammatory cytokines (IL-1β, IL-6) in macrophages induced by lipopolysaccharides (LPS) were evaluated using RT-qPCR. Results GlcN successfully mediated the synthesis of NTGlcN, which exhibited good stability. AFM characterization results revealed that NTGlcN formed tubular particles that were uniformly distributed on the surface of mica. DLS measurements indicated that the diameter of NTGlcN was approximately 15.26±3.86 nm. Cell experiments demonstrated that, compared to NTMg, macrophages exhibited a higher cellular uptake efficiency for NTGlcN, with a higher cell survival rate following treatment with NTGlcN (P < 0.05). After NTGlcN treatment, the expression of inflammatory cytokines in LPS-induced macrophages was reduced (P < 0.05). Conclusion The glucosamine/DNA composite nanostructures have been successfully developed, possessing excellent stability, biocompatibility and cell uptake efficiency. NTGlcN is capable of reducing the cytotoxicity of GlcN and can suppress cellular inflammatory responses by decreasing the expression of inflammatory cytokines in RAW264.7 cells.

Objective To explore the efficacy with prognostic value of postoperative prophylactic hyperthermic intraperitoneal chemotherapy (HIPEC) for locally advanced gastric cancer with clinical stage cT3-4NxM0. Methods A retrospective collection and analysis of clinical data was conducted from 60 patients with locally advanced gastric cancer (cT3-4NxM0) who underwent D2 radical surgery between January 2016 and December 2021 at our department. Patients who underwent prophylactic HIPEC were classified into the HIPEC group (n=30), while patients who did not undergo prophylactic HIPEC were clssified into the control group (n=30). Both groups of patients routinely were treated with postoperative adjuvant systemic chemotherapy. The median survival time, and overall survival rate of the two groups were compared through follow-up, and the risk factors affecting the overall survival rate were analyzed. Results The median survival time of the HIPEC group was 51.0 months (95% CI: 40.7-61.3), which was higher than that of the control group with 30.0 months (95% CI: 16.3-43.7). The 3-year and 5-year overall survival rates of the HIPEC group (66.7%, 53.3%)were higher than those of the control group（56.7%, 43.3%） (P=0.019). Prophylactic HIPEC was the protective factor affecting the overall survival rate of postoperative patients with locally advanced gastric cancer (P=0.021). Conclusions For patients with locally advanced cT3-4NxM0 gastric cancer, prophylactic HIPEC plays an important role in prolonging the median survival time, hence impro-ving the overall survival rate of patients.

While recent studies find that contextual diversity (CD) is a better determinant of visual word recognition than token frequency, there is a dearth of work comparing contextual diversity and token frequency in developing readers. In two sets of character and lexical decision experiments we examined token frequency and contextual diversity effects for fourth-grade children in Chinese. Experiments 1a and 1b used Chinese characters and words from SUBTLEX-CH. Experiments 2a and 2b used characters and words from a new corpus developed from Chinese primary school textbooks and reading materials from Grade 1 to 4. In both sets of experiments, CD affected character and lexical decision times but token frequency did not. The results are discussed in terms of recent context-based accounts of word learning and lexical processing, and implications are presented for models of skilled and developing reading.

Near‐infrared organic light‐emitting diodes (NIR OLEDs) with emission peak above 900 nm are attractive for many emerging applications, spanning from bioimaging to light detection and ranging. However, the device performance of NIR OLEDs is generally limited by the low quantum efficiency of emitters because of the fast nonradiative transition process imposed by energy‐gap law and aggregation quenching. So far, only a few Pt(II) complexes delivering external quantum efficiency (EQE) over 1% are reported, while there is no comparable electroluminescence in heavy‐metal‐free fluorescent organic emitters. Here, NIR OLEDs centered at 934 nm by blending an acceptor–donor–acceptor type molecule Y11 into a polymer host poly[(9,9‐dioctylfluorenyl‐2,7‐diyl)‐alt‐(4,4′‐(N‐(4‐sec‐butylphenyl)diphenylamine) (TFB) are reported. The OLEDs show a remarkably high EQE of 1.72%. Moreover, owing to a low turn‐on voltage (≈0.9 V), the resultant NIR OLEDs have an electricity‐to‐light power efficiency surpassing 20 mW W−1. The improved device performance can be attributed to enhanced photoluminescence quantum yields (PLQYs) of the blends owing to suppressed aggregation quenching, and favorable light extraction from the emissive layer. Such values are the highest among fluorescent OLEDs with electroluminescence above 900 nm. [ABSTRACT FROM AUTHOR]