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234 results on '"Xue, Hongyu"'

3. Effects of a blend of essential oils, medium-chain fatty acids, and a toxin-adsorbing mineral on diarrhea and gut microbiome of weanling pigs experimentally infected with a pathogenic Escherichia coli.

Author

He, Yijie, Jinno, Cynthia, Li, Chong, Johnston, Sara L, Xue, Hongyu, Liu, Yanhong, and Ji, Peng

Subjects
Complementary and Integrative Health, Rare Diseases, Digestive Diseases, Prevention, Nutrition, Emerging Infectious Diseases, Infectious Diseases, Animal Feed, Animals, Diarrhea, Diet, Enterotoxigenic Escherichia coli, Escherichia coli Infections, Fatty Acids, Gastrointestinal Microbiome, Minerals, Oils, Volatile, RNA, Ribosomal, 16S, Swine, Swine Diseases, Weaning, antimicrobial feed additive, diarrhea, Escherichia coli challenge, microbiome, weaned pigs, Escherichia coli challenge, Biological Sciences, Agricultural and Veterinary Sciences, Dairy & Animal Science
Abstract

A proprietary antimicrobial feed additive comprised of essential oils, medium-chain fatty acids, and a toxin-adsorbing mineral showed promising bacteriostatic and bactericidal effects in vitro. This study investigated the impacts of supplementing this blend on growth, gut microbiome, and enteric disease resilience in weaned pigs experimentally challenged with an enterotoxigenic Escherichia coli (ETEC). Thirty-six weanling pigs (6.88 ± 0.30 kg body weight) blocked by weight and gender were assigned to one of three dietary treatments: control or dietary supplementation with 0.25% or 0.50% of the antimicrobial blend. This study lasted 28 d with 7 d before and 21 d after the first ETEC inoculation (day 0). All pigs were orally inoculated with 1010 CFU F18 + ETEC/3-mL dose for 3 consecutive days. Growth performance data and diarrhea scores were recorded throughout the experiment. Fecal samples collected on days -7, 0, 7, and 21 post first inoculation (PI), and ileal digesta and mucosal tissue collected on day 21 PI were further analyzed for gut microbiome using 16S rRNA sequencing. All data, except for frequency of diarrhea and gut microbiome, were analyzed by ANOVA using the PROC MIXED of SAS. The chi-square test was used for analyzing frequency of diarrhea. Gut microbiome data were analyzed using QIIME2 and visualized using the R program. Dietary supplementation of 0.25% or 0.5% of the antimicrobial blend increased (P

Published
2022

36. Design, synthesis, biological evaluation and molecular docking study of 2,4-diarylimidazoles and 2,4-bis(benzyloxy)-5-arylpyrimidines as novel HSP90 N-terminal inhibitors

Author

Yang, Man, Li, Chenyao, Li, Yajing, Cheng, Chen, Shi, Meiyun, Yin, Lei, Xue, Hongyu, and Liu, Yajun

Abstract

The molecular chaperone HSP90 plays an essential role in cancer occurrence and development. Therefore, it is an important target for the development of anticancer drugs. 1,3-Dibenzyl-2-aryl imidazolidine (8) is a previously reported inhibitor of HSP90; however, its anticancer activity is poor. In this work, chemical modification of 8 led to the discovery of 2,4-diarylimidazoles and 2,4-bis(benzyloxy)-5-arylpyrimidines as two types of novel HSP90 N-terminal inhibitors. 16l and 22k exhibited antiproliferative activity against multiple breast cancer cell lines with IC50 values at the low micromolar level. 16l and 22k induced significant degradation of the client proteins AKT and ERK and a lower level of the heat shock response in comparison with tanespimycin (17-AAG). 22k exhibited a strong affinity for the HSP90α N-terminus with an IC50 value of 0.21 μM. A molecular docking study revealed that 16l and 22k successfully bind to the geldanamycin binding site at the N-terminus of HSP90α.

Published
2022
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37. PI3K/AKT pathway promotes keloid fibroblasts proliferation by enhancing glycolysis under hypoxia.

Author

Wang, Qifei, Yang, Xin, Ma, Jianxun, Xie, Xiang, Sun, Yimou, Chang, Xu, Bi, Hongsen, Xue, Hongyu, and Qin, Zelian

Subjects
PROTEIN analysis, WOUND healing, FIBROBLASTS, PHOSPHOTRANSFERASES, APOPTOSIS, CELLULAR signal transduction, KELOIDS, CELL proliferation, RESEARCH funding, FACTOR analysis, DESCRIPTIVE statistics, TRANSCRIPTION factors, REACTIVE oxygen species, DATA analysis software, GLYCOLYSIS, HYPOXEMIA
Abstract

Our previous study demonstrated altered glucose metabolism and enhanced phosphorylation of the PI3K/AKT pathway in keloid fibroblasts (KFb) under hypoxic conditions. However, whether the PI3K/AKT pathway influences KFb cell function by regulating glucose metabolism under hypoxic conditions remains unclear. Here, we show that when PI3K/AKT pathway was inactivated with LY294002, the protein expression of glycolytic enzymes decreased, while the amount of mitochondria and mitochondrial membrane potential increased. The key parameters of extracellular acidification rate markedly diminished, and those of oxygen consumption rate significantly increased after inhibition of the PI3K/AKT pathway. When the PI3K/AKT pathway was suppressed, the levels of reactive oxygen species (ROS) and mitochondrial ROS (mitoROS) were significantly increased. Meanwhile, cell proliferation, migration and invasion were inhibited, and apoptosis was increased when the PI3K/AKT pathway was blocked. Additionally, cell proliferation was compromised when KFb were treated with both SC79 (an activator of the PI3K/AKT pathway) and 2‐deoxy‐d‐glucose (an inhibitor of glycolysis), compared with the SC79 group. Moreover, a positive feedback mechanism was demonstrated between the PI3K/AKT pathway and hypoxia‐inducible factor‐1α (HIF‐1α). Our data collectively demonstrated that the PI3K/AKT pathway promotes proliferation and inhibits apoptosis in KFb under hypoxia by regulating glycolysis, indicating that the PI3K/AKT signalling pathway could be a therapeutic target for keloids. [ABSTRACT FROM AUTHOR]

Published
2023
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45. Metal-free C–H mercaptalization of benzothiazoles and benzoxazoles using 1,3-propanedithiol as thiol source

Author

Xiaoxia Liu, Yajun Liu, Yan Xiao, Xue Hongyu, and Bing Jing

Subjects
Letter, C–H functionalization, 1,3-propanedithiol, mercaptalization, 010402 general chemistry, 01 natural sciences, lcsh:QD241-441, chemistry.chemical_compound, lcsh:Organic chemistry, 1,3-Propanedithiol, lcsh:Science, chemistry.chemical_classification, Potassium hydroxide, 010405 organic chemistry, Organic Chemistry, benzothiazole, Benzoxazole, Combinatorial chemistry, 0104 chemical sciences, Chemistry, benzoxazole, Benzothiazole, chemistry, Metal free, Thiol, Surface modification, lcsh:Q, Reaction system
Abstract

A facile and effective C–H functionalization strategy for the synthesis of 2-mercaptobenzothiazoles and 2-mercaptobenzoxazoles is described. 1,3-Propanedithiol was employed to convert benzothiazoles and benzoxazoles to the corresponding heteroarylthiols in the presence of potassium hydroxide and DMSO. This novel protocol is featured by direct C–H mercaptalization of heteroarenes and a simple reaction system.

Published
2019

46. Strategy of Virtual Screening based Discovery of HSP90 C-terminal Inhibitors and Network Pharmacological Analysis

Author

Man Yang, Lihong Li, Chenyao Li, Yajun Liu, Xue Hongyu, and Meiyun Shi

Subjects
Virtual screening, Biological Products, biology, Pharmaceutical Science, Antineoplastic Agents, Computational biology, Molecular Dynamics Simulation, Hsp90, Molecular Docking Simulation, Network pharmacology, biology.protein, Structure based, HSP90 Heat-Shock Proteins, Biotechnology, Alternative strategy, Chemical Ingredients
Abstract

Background: HSP90 has been considered an important anticancer target for several decades, but traditional HSP90 N-terminal inhibitors often suffered from organ toxicity and/or drug resistance. Methods: The development of HSP90 C-terminal inhibitors represents a reliable alternative strategy. In view of rare examples of structure-based identification of HSP90 C-terminal inhibitors, we report a virtual screening based strategy for the discovery of HSP90 C-terminal inhibitors as anticancer agents from natural products. Results & Discussion: 13 chemical ingredients from licorice were identified as possible HSP90 inhibitors and 3 of them have been reported as anticancer agents. The binding modes towards HSP90 C-terminus were predicted by molecular docking and refined by molecular dynamics simulation. Conclusion: Further network pharmacological analysis predicted overall possible targets involved in the pathways in cancer and revealed that 8 molecules possibly interact with HSP90. A structure based virtual screening strategy was established for the discovery of HSP90 Cterminal inhibitors.

Published
2021

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