1. MicroRNA-214 is aberrantly expressed in cervical cancers and inhibits the growth of HeLa cells
- Author
-
Min Liu, Shuang Chen, Zuozhen Yang, Tao Liu, Hua Tang, Yixuan Li, Xin Li, and Xuejing Luan
- Subjects
Adult ,MAP Kinase Kinase 3 ,Clinical Biochemistry ,Down-Regulation ,Uterine Cervical Neoplasms ,Biology ,Biochemistry ,HeLa ,Downregulation and upregulation ,microRNA ,Genetics ,Humans ,Mitogen-Activated Protein Kinase 8 ,RNA, Messenger ,miR-214 ,Molecular Biology ,Gene ,Cell Proliferation ,Messenger RNA ,Cell growth ,RNA ,Cell Biology ,Middle Aged ,biology.organism_classification ,Cell biology ,MicroRNAs ,Female ,HeLa Cells - Abstract
MicroRNAs are a group of endogenously expressed, single-stranded, 18-24 nt RNAs that regulate diverse cellular pathways. Although documented evidence indicates that some microRNAs can function as oncogenes or tumor-suppressors, the role of miR-214 in regulating human cervical cancer cells remains unexplored. We determined the expression level of miR-214 and found it is downregulated in cervical cancer compared with normal tissue. Overexpression of miR-214 in HeLa cells, a human cervical cancer cell line, significantly inhibited cell proliferation according to the MTT and colony forming assays. HeLa cells that stably overexpress miR-214 downregulate the expression of MEK3 and JNK1 at both mRNA and protein levels. Further investigation revealed that miR-214 regulates the expression of MEK3 and JNK1 by targeting the 3'UTRs of these genes. Collectively, these results suggest that miR-214 negatively regulates HeLa cell proliferation by targeting the noncoding regions of MEK3 and JNK1 mRNAs.
- Published
- 2009