Search

Although several pentatricopeptide repeat (PPR) proteins are involved in post-transcriptional processing of mitochondrial RNA, it is unclear which specific protein is involved in the RNA editing of ccmC in maize (Zea mays). Here we report the identification of the maize empty pericarp 601 (emp601) mutant and the map-based cloning of the Emp601 gene, which encodes an E2-type PPR protein that is targeted to mitochondria. A single-nucleotide deletion in the emp601 mutant caused a frameshift and introduced a premature stop codon into the predicted EMP601. This mutation was associated with reduced accumulation of mitochondrial complex III as well as with inhibition of growth and differentiation of basal endosperm transfer layer cells, leading to final degeneration of the embryo and endosperm. We determine that loss of EMP601 function prevents the C-to-U RNA editing of the mitochondrial transcript ccmC at position 358. EMP601 binds to the ccmC transcript and directly interacts with Multiple organellar RNA editing factor 8 and may be a component of the plant mitochondrial editosome. We conclude that EMP601 functions in RNA editing of mitochondrial ccmC transcripts and influences mitochondrial function and seed development.

Plant seedling morphogenesis is considerably related to photosynthesis, pigment synthesis, and circadian periodicity during seedling development. We identified and cloned a maize zebra or crossbanding leaves mutant wk3735, which produces pale white kernels and was identified and plays a role in the equilibrium of the Redox state the in/out of ETC by active oxygen scavenging. Interestingly, it produces the zebra leaves during the production of the first seven leaves, which is apparently different from the mutation of homologs AtPTOX in Arabidopsis. It is intriguing to investigate how and why yellow crossbands (zebra leaf phenotype) emerge on leaves. As expected, chlorophyll concentration and photosynthetic efficiency both significantly declined in the yellow sector of wk3735 leaves. Meanwhile, we observed the circadian expression pattern of ZmPTOX1, which was further validated by protein interaction assays of the circadian clock protein TIM1 and ZmPTOX1. The transcriptome data of yellow (muW) and green (muG) sectors of knock-out lines and normal leaves of overexpression lines (OE) at the 5th-leaf seedling stage were analyzed. Zebra leaf etiolated sections exhibit a marked defect in the expression of genes involved in the circadian rhythm and rhythmic stress (light and cold stress) responses than green sections. According to the analysis of co-DEGs of muW vs. OE and muG vs. OE, terms linked to cell repair function were upregulated while those linked to environmental adaptability and stress response were downregulated due to the mutation of ZmPTOX1. Further gene expression level analyses of reactive oxygen species (ROS) scavenging enzymes and detection of ROS deposition indicated that ZmPTOX1 played an essential role in plant stress resistance and ROS homeostasis. The pleiotropic roles of ZmPTOX1 in plant ROS homeostasis maintenance, stress response, and circadian rhythm character may collectively explain the phenotype of zebra leaves during wk3735 seedling development.

Objectives Combination of Breast Cancer 1 protein-associated protein 1 (BAP1) and methylthioadenosine phosphorylase (MTAP) in the peritoneal mesothelioma (PeM) has yet to be explored. We aim to assess the diagnostic value of combined BAP1 and MTAP to distinguish biphasic mesothelioma (BM) from epithelioid mesothelioma (EM) with reactive stroma in peritoneum, as well as its prognostic value in PeM. Methods This is a retrospective study from June 2014 to December 2021. This study included 18 cases of BM and 27 cases of EM with reactive stroma, excluded sarcomatoid, and EM without reactive stroma cases, and clinicopathological information was collected. The associations between MTAP and BAP1 levels and clinicopathological features or prognosis were analyzed. Clinical follow-up data were reviewed to correlate with pathological prognostic factors using Kaplan–Meier estimator and univariate/multivariate Cox proportional hazards regression models. Results Loss/decrease of BAP1/MTAP was observed in 6 (33.3%) BM cases and 12 (44.4%) EM cases. In 5 (27.8%) cases, loss of or decreased BAP1/MTAP expression was observed in both EC and SC of BM. BAP1/MTAP loss/decrease was observed in 12 (44.4%) cases of only EC of EM but not in reactive stroma. Compared with histology alone, a combination of BAP1 and MTAP immunohistochemistry (IHC) in spindled PeM provides a more objective mean to distinguish BM from EM with reactive stroma. Loss/decrease of BAP1/MTAP was associated with peritoneal cancer index (PCI) score ( P = 0.047) and completeness of cytoreduction (CC) score ( P = 0.038). BM patients have worse overall survival (OS) than EM with reactive stroma ( P = 0 .007). Conclusions Combination of BAP1/MTAP by IHC is helpful for differential diagnosis of peritoneal BM from EM with reactive stroma. Nevertheless, BAP1/MTAP may help to evaluate the biological behavior of PeM.

BackgroundAlzheimer’s disease is a common neurodegenerative disease, and patients with early-onset Alzheimer’s disease (onset age

Metasurface lenses (or metalenses) have aroused great attentions and efforts in the community of metamaterials or metasurfaces due to its ultrathin device dimension and superior focusing performances. High-efficiency transmissive metalenses with an ultra-thin device thickness are an important aspect especially in the low frequency by using plasmonic transmitarray antennas. In this paper, an ultra-thin plasmonic metalens with only 0.1λ (λ is working wavelength, the aperture size is 7λ) device thickness is designed by changing the radius of the proposed symmetric complementary split ring resonator antenna metasurfaces. Thanks to its high transmittance and large phase shift of the plasmonic meta-atoms, the designed metalens achieves both high transmissive efficiency of 80% and high focusing efficiency of 50% on the focal plane of F = 4.6λ in the simulations. The designed ultra-thin plasmonic metalens has a moderate large numerical aperture of 0.67 (NA = 0.67). In order to verify its high working efficiency of the proposed plasmonic metalens, a sample is also fabricated and a much higher focusing efficiency of 65% is realized in the measurements. The influence of the open angles of the symmetric split ring transmitarray metasurface on the focusing performances such as working efficiency and NA of the designed metalens is also studied and analyzed finally, which can add new degree of freedoms to optimize its focusing performance. The presented studies can facilitate the development of high-efficiency metalenses in the low frequency and have significant potential applications in high-resolution microwave imaging, high-gain metalens antennas and others.

In recent years, the tourism industry has developed rapidly. However, traditional tourism information has the disadvantages of slow response speed and limited information content, which cannot reflect the evolution trend of spatial and temporal patterns of tourism information in time. Here, based on the Baidu Index, we construct an evaluation framework to analyse the spatial and temporal flow of tourism information in the Chengdu–Chongqing urban cluster from 2011 to 2021. Then, we analyse the urban links between different network levels from the evolution pattern. Finally, we use the geodetector model to analyse its driving mechanism. The results show that Chengdu and Chongqing are the most active cities in the study area in terms of tourism information. The unbalanced development of tourism information between Chengdu and Chongqing and other cities in the region gradually deepens during the period 2011–2019 (polarization effect), but the unbalanced development moderates after 2019. On the other hand, cities in the middle of the Chengdu–Chongqing cluster always have weak agglomeration effects of tourism information. Cities with high tourism information outflow rates in the Chengdu–Chongqing city cluster are mainly concentrated around Chengdu. The average outflow rate of Deyang is the highest, at 27.8%. Cities with low tourist information outflow rates are primarily located in the west, central and south. Ya’an is the city with the lowest outflow rate, with an average of −62.2%. Specifically, Chengdu is the dominant and most radiantly influential city. The tourism information of the Chengdu–Chongqing urban cluster shows a radial network with Chengdu and Chongqing as the core. The driving force analysis shows that the push factor of tourist source, such as the number of people buying pension insurance, is the core driving mechanism, while the pull factor of destination, such as the park green area, and resistance factors such as psychological distance, are in the secondary position. In general, this paper uses Internet tourism data to expand the traditional tourism information research of the Chengdu–Chongqing urban cluster, which can better respond to the changes and needs of the tourism market and provide reference for the spatial optimization of tourism destinations.

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that affects millions of people worldwide. Positron emission tomography/magnetic resonance (PET/MR) imaging is a promising technique that combines the advantages of PET and MR to provide both functional and structural information of the brain. Deep learning (DL) is a subfield of machine learning (ML) and artificial intelligence (AI) that focuses on developing algorithms and models inspired by the structure and function of the human brain’s neural networks. DL has been applied to various aspects of PET/MR imaging in AD, such as image segmentation, image reconstruction, diagnosis and prediction, and visualization of pathological features. In this review, we introduce the basic concepts and types of DL algorithms, such as feed forward neural networks, convolutional neural networks, recurrent neural networks, and autoencoders. We then summarize the current applications and challenges of DL in PET/MR imaging in AD, and discuss the future directions and opportunities for automated diagnosis, predictions of models, and personalized medicine. We conclude that DL has great potential to improve the quality and efficiency of PET/MR imaging in AD, and to provide new insights into the pathophysiology and treatment of this devastating disease.

We propose and demonstrate a stable single-longitudinal mode (SLM) thulium-doped fiber laser (TDFL) with a Fabry- Pérot (F-P) fiber Bragg grating (FBG) filter and a triple-coupler based double-ring cavity (TC-DRC) filter. For the first time, this structure of TC-DRC filter is used to select a single mode from dense longitudinal-modes in a ring cavity TDFL. The design and fabricate methods of TC-DRC filter are revealed and the principle of SLM selection is also analyzed in detail. The experimental results demonstrated the good performance of the proposed filter. The central wavelength of proposed TDFL is 2049.49 nm and its OSNR is higher than 35 dB. There is no obvious wavelength drift during the test, and the power fluctuation is less 0.5 dB. The SLM operation is verified through the self-homodyne method, this laser can be stably maintained in a SLM state after operating for one hour under laboratory condition. In addition, the linewidth is measured less than 10 kHz based on the phase noise demodulation method.

A switchable multi-wavelength thulium-doped fiber laser (TDFL) using either two-stage cascaded or two-segment Sagnac loop filters are proposed and analyzed. Both filters incorporate a 3-m and 1.7-m long polarization-maintaining fiber, which acts as a comb filter. By adjusting two polarization controllers, when the TDFL used a cascaded Sagnac loop filter, eight stable single-wavelength operations were obtained with easy switching among them. The wavelength range was 16.36 nm during the single-wavelength switchable operation, with the optical signal-to-noise ratio up to 55 dB. This TDFL can achieve at most quintuple-wavelength operation. When the TDFL used a two-segment Sagnac loop filter, we achieved single-wavelength and dual-wavelength switchable. This TDFL can achieve at most stable quadruple-wavelength operation. In dual-wavelength switchable operation, the maximum and minimum wavelength spacings were 21.78 nm and 1.50 nm. Due to its abundant range of wavelengths, the proposed TDFLs has great potential in wavelength-division multiplexing.

BackgroundStewart–Treves Syndrome in Primary Limb Lymphedema (STS-PLE) is an extremely rare malignant tumor. A retrospective analysis was conducted to elucidate the relationship between magnetic resonance imaging (MRI) findings and signs compared to pathology.MethodsSeven patients with STS-PLE were enrolled at Beijing Shijitan Hospital, Capital Medical University, from June 2008 to March 2022. All cases were examined by MRI. The surgical specimens were subjected to histopathological and immunohistochemical staining for CD31, CD34, D2-40, and Ki-67.ResultsThere were two different types of MRI findings. One was mass shape (STS-PLE I type) in three male patients, and the other was the “trash ice” d sign (STS-PLE II type) observed in four female patients. The average duration of lymphedema (DL) of STS-PLE I type (18 months) was shorter than that of STS-PLE II type (31 months). The prognosis for the STS-PLE I type was worse than that for the STS-PLE II type. Regarding overall survival (OS), the STS-PLE I type (17.3 months) was three times shorter than that of the STS-PLE II type (54.5 months). For STS-PLE I type, the older the STS-PLE onset, the shorter the OS. However, there was no significant correlation in STS-PLE II type. MRI was compared to histological results to provide an explanation for the differences in MR signal changes, especially on T2WI. Against a background of dense tumor cells, the richer the lumen of immature vessels and clefts, the higher the T2WI MRI signal (taking muscle signal as the internal reference standard) and the worse the prognosis, and vice versa. We also found that younger patients with a lower Ki-67 index (

Anthocyanin, a kind of flavonoid, plays a crucial role in plant resistance to abiotic stress. Salt stress is a kind of abiotic stress that can damage the growth and development of plant seedlings. However, limited research has been conducted on the involvement of maize seedlings in salt stress resistance via anthocyanin accumulation, and its potential molecular mechanism is still unclear. Therefore, it is of great significance for the normal growth and development of maize seedlings to explore the potential molecular mechanism of anthocyanin improving salt tolerance of seedlings via transcriptome analysis. In this study, we identified two W22 inbred lines (tolerant line pur–W22 and sensitive line bro–W22) exhibiting differential tolerance to salt stress during seedling growth and development but showing no significant differences in seedling characteristics under non–treatment conditions. In order to identify the specific genes involved in seedlings’ salt stress response, we generated two recombinant inbred lines (RILpur–W22 and RILbro–W22) by crossing pur–W22 and bro–W22, and then performed transcriptome analysis on seedlings grown under both non–treatment and salt treatment conditions. A total of 6100 and 5710 differentially expressed genes (DEGs) were identified in RILpur–W22 and RILbro–W22 seedlings, respectively, under salt–stressed conditions when compared to the non–treated groups. Among these DEGs, 3160 were identified as being present in both RILpur–W22 and RILbro–W22, and these served as commonly stressed EDGs that were mainly enriched in the redox process, the monomer metabolic process, catalytic activity, the plasma membrane, and metabolic process regulation. Furthermore, we detected 1728 specific DEGs in the salt–tolerant RILpur–W22 line that were not detected in the salt–sensitive RILbro–W22 line, of which 887 were upregulated and 841 were downregulated. These DEGs are primarily associated with redox processes, biological regulation, and the plasma membrane. Notably, the anthocyanin synthesis related genes in RILpur–W22 were strongly induced under salt treatment conditions, which was consistented with the salt tolerance phenotype of its seedlings. In summary, the results of the transcriptome analysis not only expanded our understanding of the complex molecular mechanism of anthocyanin in improving the salt tolerance of maize seedlings, but also, the DEGs specifically expressed in the salt–tolerant line (RILpur–W22) provided candidate genes for further genetic analysis.

Background: The aim of this study is to search for the predictive value of 3D fat analysis and calculation technique (FACT) and intravoxel incoherent motion (IVIM) parameters in identifying osteoporosis in women. Methods: We enrolled 48 female subjects who underwent 3.0 T MRI, including 3D FACT and IVIM sequences. Bone mineral density (BMD) values and Fracture Risk Assessment (FRAX) scores were obtained. Proton density fat fraction (PDFF) in the bone marrow and the real diffusion (D) value of intervertebral discs were measured on 3D FACT and IVIM images, respectively. Accuracy and bias were assessed by linear regression analysis and Bland–Altman plots. Intraclass correlation coefficients were used to assess the measurements’ reproducibility. Spearman’s rank correlation was applied to explore the correlation. MRI-based parameters were tested for significant differences among the three groups using ANOVA analyses. A receiver operating characteristic (ROC) analysis was performed. Results: The PDFF of the vertebral body showed a negative correlation with BMD (R = −0.393, p = 0.005) and a positive correlation with the FRAX score (R = 0.706, p < 0.001). The D value of intervertebral discs showed a positive correlation with BMD (R = 0.321, p = 0.024) and a negative correlation with the FRAX score (R = −0.334, p = 0.019). The area under the curve values from the ROC analysis showed that the 3D FACT and IVIM sequences could accurately differentiate between normal and osteoporosis (AUC = 0.88 using the PDFF; AUC = 0.77 using the D value). The PDFF value demonstrated a sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 78.6%, 89.5%, 84.6%, and 85.0%, respectively, in its ability to predict osteoporosis. The D value had a sensitivity, specificity, PPV, and NPV of 63.16%, 92.9%, 65.0%, and 77.8%, respectively, for predicting osteoporosis. Conclusions: The 3D FACT- and IVIM-measured PDFF and D values are promising biomarkers in the assessment of bone quality and fracture risk.

Drought is one of the most critical abiotic stresses influencing maize yield. Improving maize cultivars with drought tolerance using marker-assisted selection requires a better understanding of its genetic basis. In this study, a doubled haploid (DH) population consisting of 217 lines was created by crossing the inbred lines Han 21 (drought-tolerant) and Ye 478 (drought-sensitive). The population was genotyped with a 6 K SNP assay and 756 SNP (single nucleotide polymorphism) markers were used to construct a linkage map with a length of 1344 cM. Grain yield (GY), ear setting percentage (ESP), and anthesis–silking interval (ASI) were recorded in seven environments under well-watered (WW) and water-stressed (WS) regimes. High phenotypic variation was observed for all traits under both water regimes. Using the LSMEAN (least-squares mean) values from all environments for each trait, 18 QTL were detected, with 9 associated with the WW and 9 with the WS regime. Four chromosome regions, Chr. 3: 219.8–223.7 Mb, Chr. 5: 191.5–194.7 Mb, Chr. 7: 132.2–135.6 Mb, and Chr. 10: 88.2–89.4 Mb, harbored at least 2 QTL in each region, and QTL co-located in a region inherited favorable alleles from the same parent. A set of 64 drought-tolerant BC3F6 lines showed preferential accumulation of the favorable alleles in these four regions, supporting an association between the four regions and maize drought tolerance. QTL-by-environment interaction analysis revealed 28 edQTL (environment-dependent QTL) associated with the WS regime and 22 associated with the WW regime for GY, ESP, and ASI. All WS QTL and 55.6% of WW QTL were located in the edQTL regions. The hotspot genomic regions identified in this work will support further fine mapping and marker-assisted breeding of drought-tolerant maize.

BackgroundEosinophilic leukemia (EL) is a rare, serious and potentially life-threatening condition characterized by the overproduction of eosinophils leading to tissue eosinophilic infiltration and damage. Although multiple organ systems may be involved, progressive eosinophilic myocarditis (EM) is the most common cause of morbidity and mortality. Early diagnosis and follow-up surveillance combined with multimodal imaging are crucial for appropriate treatment of EM.Case SummaryIt’s a rare case of EL with EM and intracardiac thrombus in a 59-year-old patient who presented with asthenia for 3 weeks. Full blood count analysis indicated significant eosinophilia. Bone marrow aspirate revealed dysplastic eosinophilia and a FIP1L1-PDGFRA fusion gene (4q12) was detected, confirming EL. Echocardiography revealed EM with intracardiac thrombus. This was later confirmed by cardiac magnetic resonance imaging. The patient was commenced on imatinib and prednisolone and good clinical response was obtained. Through 18F-FAPI PET/CT imaging, we obtained in vivo visualization of fibroblast activation changes in the early stage of cardiac structure remodeling. With anti-fibrotic therapy after heart failure, the patient achieved a good clinical response.ConclusionThis case demonstrates in vivo visualization of fibroblast activation after EM. Multimodality imaging can provide early diagnosis and may guide tailored antifibrotic therapy in early stage of EM.

Switchable metamaterial absorbers/reflectors (MAs/MRs) are important bifunctional electromagnetic devices and have been the subject of numerous scientific studies. However, there is a lack of bifunctional devices that operate in the terahertz band. Here, we theoretically propose a broadband switchable MA with many excellent properties, such as good thermal stability, high insensitivity to inferior film quality of the graphene, excitation polarization and wide incident angles, and outstanding structural parameter tolerance. The bandwidth of the proposed broadband MA is 3.4 THz with an absorptivity over 90% in the frequency band of 1.6-5 THz. The proposed absorber can switch to a reflector with a reflectivity over 93% by tuning the chemical potential of the graphene and reducing the temperature. Therefore, the switching intensity of the proposed MA exceeds 83%. The physical mechanisms of the broadband absorption of the proposed structure are investigated using the impedance matching theory and the multiple reflection interference theory. The reflection mechanism of the proposed broadband reflector is discussed by analyzing the effective parameters. The absorption and switching mechanism are theoretically investigated by performing detailed numerical calculations to analyze the surface loss intensity, electric field, and magnetic field. These findings can accelerate the development of terahertz broadband switchable devices.

The special electromagnetic properties of metamaterials have contributed to the development of terahertz technology, and terahertz broadband absorbers for various applications have been investigated. The design of metamaterial absorbers with tunability is in a particularly attractive position. In this work, a tunable broadband terahertz metamaterial absorber is proposed based on the phase transition material vanadium dioxide (VO2). The simulation results show that an excellent absorption bandwidth reaches 3.78 THz with the absorptivity over 90% under normal incidence. The absorptivity of the proposed structure can be dynamically tuned from 2.7% to 98.9% by changing the conductivity of VO2, which changes the structure from a perfect reflector to an absorber. An excellent amplitude modulation with the absorptivity is realized. The mechanism of broadband absorption is explored by analyzing the electric field distribution of the absorber based on impedance matching theory. In addition, it also has the advantage of polarization and incident angle insensitivity. The proposed absorber may have a wide range of promising applications in areas such as terahertz imaging, sensing, and detection.

Typically, sweet corn, particularly sh2 sweet corn, has low seed vigor owing to its high sugar and low starch content, which is a major problem in sweet corn production, particularly at low temperatures. There is considerable variation in the germination rates among sweet corn varieties under low-temperature conditions, and the underlying mechanisms behind this phenomenon remain unclear. In this study, we screened two inbred sweet corn lines (tolerant line L282 and sensitive line L693) differing in their low-temperature germination rates; while no difference was observed in their germination rates at normal temperatures. To identify the specifically induced genes influencing the germination capacity of sweet corn at low temperatures, a transcriptome analysis of the two lines was conducted at both normal and low temperatures. Compared to the lines at a normal temperature, 3926 and 1404 differently expressed genes (DEGs) were identified from L282 and L693, respectively, under low-temperature conditions. Of them, 830 DEGs were common DEGs (cDEGs) that were identified from both L282 and L693, which were majorly enriched in terms of microtubule-based processes, histone H3-K9 modification, single-organism cellular processes, and carbohydrate metabolic processes. In addition, 3096 special DEGs (sDEGs), with 2199 upregulated and 897 downregulated, were detected in the tolerant line L282, but not in the sensitive line L693. These sDEGs were primarily related to plasma membranes and oxygen-containing compounds. Furthermore, electric conductivity measurements demonstrated that the membrane of L282 experienced less damage, which is consistent with its strong tolerance at low temperatures. These results expand our understanding of the complex mechanisms involved in the cold germination of sweet corn and provide a set of candidate genes for further genetic analysis.

Although bulked segregant analysis (BSA) has been used extensively in genetic mapping, user-friendly tools which can integrate current algorithms for researchers with no background in bioinformatics are scarce. To address this issue, we developed an R package, PNGSeqR, which takes single-nucleotide polymorphism (SNP) markers from next-generation sequencing (NGS) data in variant call format (VCF) as the input file, provides four BSA algorithms to indicate the magnitude of genome-wide signals, and rapidly defines the candidate region through the permutation test and fractile quantile. Users can choose the analysis methods according to their data and experimental design. In addition, it also supports differential expression gene analysis (DEG) and gene ontology analysis (GO) to prioritize the target gene. Once the analysis is completed, the plots can conveniently be exported.

Maize originated from tropical regions and is extremely sensitive to low temperature during germination. Our previous work identified a major QTL, qp1ER1-1, for low temperature germination ability (LTGA) of maize. Here, we introgressed qp1ER1-1 from the tolerant line L220 into the sensitive line PH4CV to generate two near isogenic lines NIL220-3 and NIL220-25. When germinated under cold temperature for 25 days (Cold-25), the NILs showed similar seedling root length and shoot length to L220, but significantly higher than PH4CV. However, when germinated under cold temperature for 15 days (Cold-15) or under normal temperature (25 °C) for 3 days (CK-3), all lines showed similar seedling performance, indicating that introgression of qp1ER1-1 from L220 into PH4CV could improve LTGA of NIL220-3 and NIL220-25. The whole seedlings, including root and shoot, of Cold-15 and CK-3 were harvested for transcriptome analysis, when both stayed at a similar developmental stage. Dry seed embryo was sequenced as a non-germination control (CK-0). Compared with PH4CV, the tolerant line (L220, NIL220-3, and NIL220-25) specifically expressed genes (different expressed genes, DEGs) were identified for CK-0, Cold-15, and CK-3. Then, DEGs identified from Cold-15, but not from CK-0 or CK-3, were defined as tolerant line specifically expressed LTGA genes. Finally, 1786, 174, and 133 DEGs were identified as L220, NIL220-3, and NIL220-25 specifically expressed LTGA genes, respectively. Of them, 27 were common LTGA genes that could be identified from all three tolerant lines, with two (Zm00001d031209 and Zm00001d031292) locating in the confidence interval of qp1ER1-1. In addition, GO analysis revealed that L220 specifically expressed LTGA genes were majorly enriched in the cell division process and plasma membrane related categories. Taken together, these results provided new insight into the molecular mechanism of maize seed LTGA and facilitated the cloning of the qp1ER1-1 gene.

Abstract Callus induction is a dedifferentiation process that accompanies a cell fate transition, and epigenetic regulation plays a crucial role in the process. N6‐methyladenosine (m6A) methylation is an important mechanism in post‐transcriptional epigenetic regulation and functions in cell reprogramming. However, the function of m6A methylation during callus induction is still unknown. Here, we performed transcriptome‐wide m6A‐seq on immature maize embryos after culturing for 2, 4, or 8 days with or without the auxin analogue 2,4‐D. A total of 26,794 unique m6A peaks were detected from 17,456 maize genes; and 2,338 specific, 2,4‐D‐induced m6A peaks (D‐specific m6A) were detected only in embryos cultured with 2,4‐D. Furthermore, a positive correlation between m6A methylation and mRNA abundance was discovered in the genes with D‐specific m6A deposition, especially at the beginning of callus induction. Key genes involved in callus induction, i.e. BABY BOOM and LBD transcription factors, underwent m6A methylation, increasing their transcript levels, thus improving callus induction. These results revealed the importance of m6A methylation during the early stage of callus induction and provided new insights into the molecular mechanism of callus induction at an epitranscriptomic level.

In order to widen the absorption band of the metamaterial absorber to expand its application field, using the electromagnetic characteristics of water in the microwave band, a water-based metamaterial absorber with a structure different from the traditional “metal layer–dielectric layer–metal layer” one is proposed. The numerical simulation results show that its absorptivity of incident electromagnetic waves exceeds 90% in the ultra-wide operating band of 7.84–74.16 GHz and the absorption bandwidth can cover five bands including the X-band, Ku-band, K-band, Ka-band, and U-band. Based on the theories of equivalent medium and impedance matching, the mechanism of broadband absorption was explored by analyzing the electromagnetic field distribution and power loss density of the absorber. The designed absorber has the absorption performance of polarization independence, and it can work under a wide incident angle. In addition, the absorber has excellent thermal stability which can still maintain stable operation when the temperature changes from 0 °C to 100 °C. This water-based metamaterial absorber is of low cost, and its manufacturing process is relatively convenient, so it has broad application prospects in the fields of radar stealth, microwave darkroom, and prevention of electromagnetic radiation.

Maize transformation is highly based on the formation of embryonic callus, which is mainly derived from scutellum cells of the immature maize embryo. However, only a few genes involved in callus induction have been identified in maize. To reveal the potential genes involved in the callus induction of maize, we carried out a high-throughput RNA sequencing on embryos that were cultured for 0, 1, 2, 4, 6, and 8 days, respectively, on a medium containing or lacking 2,4-dichlorophenoxyacetic acid. In total, 7,525 genes were found to be induced by 2,4-dichlorophenoxyacetic acid and categorized into eight clusters, with clusters 2 and 3 showing an increasing trend related to signal transmission, signal transduction, iron ion binding, and heme binding. Among the induced genes, 659 transcription factors belong to 51 families. An AP2 transcription factors, ZmBBM2, was dramatically and rapidly induced by auxin and further characterization showed that overexpression of ZmBBM2 can promote callus induction and proliferation in three inbred maize lines. Therefore, our comprehensive analyses provide some insight into the early molecular regulations during callus induction and are useful for further identification of the regulators governing callus formation.

Abstract Metastasis is the leading cause of death in breast cancer patients. However, the mechanisms underlying metastasis are not well understood and there is no effective treatment in the clinic. Here, we demonstrate that in MMTV-PyMT, a highly malignant spontaneous breast tumor model, IL-25 (also called IL-17E) was expressed by tumor-infiltrating CD4+ T cells and macrophages. An IL-25 neutralization antibody, while not affecting primary tumor growth, substantially reduced lung metastasis. Inhibition of IL-25 resulted in decreased type 2 T cells and macrophages in the primary tumor microenvironments, both reported to enhance breast tumor invasion and subsequent metastasis to the lung. Taken together, our data suggest IL-25 blockade as a novel treatment for metastatic breast tumor.