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316L SS is widely used but is prone to algae adhesion, making biofouling a big challenge for the long-term functionality and reliability in freshwater. Most reported organic antifouling coatings are not suitable for use in freshwater environments, making copper-containing stainless steels a good candidate for freshwater antifouling. Anti-fouling resistance of 316L-Cu SS and 316L SS was compared using a self-built natural simulation ecosystem in this study. The results demonstrated that the algae adhesion percentage on 316L-Cu SS is much lower than that on 316L SS from both macro and micro perspectives using a single-lens reflex camera and high sensitivity structured illumination microscope. Results of this study clearly revealed that the mechanism of algae resistance for 316L-Cu SS is the release of Cu2+ can inhibit the adhesion of proteins and polysaccharides, weakening the adhesion of the extracellular polymeric substances to the metal substrate and even make the attached diatoms fracture or damage. This study provides a novel strategy to prevent algae adhesion on metal surface for an array of underwater equipment used in freshwater and contributes towards the advancement of materials used for underwater equipment, fostering enhanced performance and reduced maintenance in freshwater applications and the mitigation of algae fouling in aquatic environments.

The sintering process of Cu nanoparticle (Cu NP)/graphene nanoplatelet (GNP) composite solder paste was thoroughly investigated in this work through molecular dynamics simulations. The tensile properties of the sintered Cu NP/GNP composite solder paste were considered by using the uniaxial quasi-static tensile simulation method. The impact of sintering temperature, strain rate, and GNP addition on the tensile properties of Cu NP/GNP sintered structures was thoroughly investigated. The lattice structure, dislocation evolution, and atomic diffusion of the molecular dynamics results were analyzed using the common neighbor analysis (CNA), dislocation extraction algorithm (DXA), and mean square displacement (MSD) methods. The results of the post-processing analysis showed that the addition of GNP and the sintering temperature have an important influence on the mechanical properties of Cu NP/GNP sintered structures. In addition, the incorporation of GNP can significantly improve the mechanical properties of sintered Cu NP/GNP composite solder paste. More specifically, the tensile strength and fracture strain of the sintered composite solder paste will be increased by increasing the tensile strain rate. The strengthening mechanism of the sintered Cu NP/GNP composite solder paste can be attributed to the dislocation strengthening mechanism. Our study provides valuable insight for the development of high-performance composite solder paste with enhanced mechanical properties.

2-methylisoborneol (2-MIB), a secondary metabolite produced by cyanobacteria, often causes a musty odour in water, threatening the safety of drinking water supplies. This study investigated the effects of the growth phases on the production of 2-MIB by Pseudanabaena. The effects of cell characteristics on the production and release of 2-MIB were also explored. The total 2-MIB concentration increased during the exponential phase and decreased during the declining phase, which was consistent with the changes in cell density. However, the total 2-MIB yield (1.12–1.27 fg cell−1) of Pseudanabaena did not significantly differ throughout the growth cycle (p > 0.05). Meanwhile, the extracellular 2-MIB yield increased significantly from 0.31 fg cell−1 in the exponential phase to 0.76 fg cell−1 in the declining phase (p < 0.05), and the corresponding proportion of extracellular 2-MIB improved from 25.13% to 59.16% (p < 0.05). The surge in extracellular 2-MIB during the declining phase could be attributed to the breaking of the Pseudanabaena filament, as indicated by the decrease in Dmean during cell ageing. The findings of this study contribute to a more inclusive comprehension and management of musty odour issues resulting from cyanobacteria in the water supply.

BackgroundHelicobacter pylori (HP) is considered a leading risk factor for gastric cancer (GC). The aim of this article is to conduct bibliometric and visual analysis to assess scientific output, identify highly cited papers, summarize current knowledge, and explore recent hotspots and trends in HP/GC research.MethodsA bibliographic search was conducted on October 24, 2023, to retrieve relevant studies on HP/GC research between 2003 and 2022. The search terms were attached to HP and GC. The main data were from the Web of Science Core Collection (WoSCC). Data visualization was performed using Biblioshiny, VOSviewer, and Microsoft Excel.ResultsIn HP/GC research, 1970 papers were retrieved. The total number of papers (Np) in HP/GC was growing from 2003 to 2022. China and Japan were in the leading position and made the most contributions to HP/GC. Vanderbilt University and the US Department of Veterans Affairs had the highest Np. The most productive authors were Peek Jr Richard M. and Piazuelo M Blanca. Helicobacter received the most Np, while Gastroenterology had the most total citations (TC). High-cited publications and keyword clustering were used to identify the current status and trends in HP/GC research, while historical citation analysis provided insight into the evolution of HP/GC research. The hot topics included the effect of HP on gastric tumorigenesis and progression, the pathogenesis of HP-induced GC (HP factors), and the mechanisms by which HP affects GC (host factors). Research in the coming years could focus on topics such as autophagy, gut microbiota, immunotherapy, exosomes, epithelial-mesenchymal transition (EMT), and gamma-glutamyl transpeptidase (GGT).ConclusionThis study evaluated the global scientific output in HP/GC research and its quantitative characteristics, identified the essential works, and collected information on the current status, main focuses and emerging trends in HP/GC research to provide academics with guidance for future paths.

ABSTRACTObservational studies have shown that the gut microbiome is associated with frailty. However, whether these associations underlie causal effects remains unknown. Thus, this study aimed to assess the genetic correlation and causal relationships between the genetically predicted gut microbiome and frailty using linkage disequilibrium score regression (LDSC) and Mendelian Randomization (MR). Summary statistics for the gut microbiome were obtained from a genome-wide association study (GWAS) meta-analysis of the MiBioGen consortium (N = 18,340). Summary statistics for frailty were obtained from a GWAS meta-analysis, including the UK Biobank and TwinGene (N = 175,226). We used LDSC and MR analyses to estimate the genetic correlation and causality between the genetically predicted gut microbiome and frailty. Our findings indicate a suggestive genetic correlation between Christensenellaceae R-7 and frailty. Moreover, we found evidence for suggestive causal effects of twelve genus-level gut microbes on frailty using at least two MR methods. There was no evidence of horizontal pleiotropy or heterogeneity in the MR analysis. This study provides suggestive evidence for a potential genetic correlation and causal association between several genetically predicted gut microbes and frailty. More population-based observational studies and animal experiments are required to clarify this association and the underlying mechanisms.

Abstract Background Ferroptosis is iron-dependent non-apoptotic cell death, that is characterized by the excessive accumulation of lipid peroxides. Ferroptosis-inducing therapy also shows promise in the treatment of cancers. However, ferroptosis-inducing therapy for glioblastoma multiforme (GBM) is still in the exploratory stage. Methods We identified the differentially expressed ferroptosis regulators using Mann–Whitney U test in the proteome data from Clinical Proteomic Tumor Analysis Consortium (CPTAC). We next analyzed the effect of mutation on protein abundance. A multivariate Cox model was constructed to identify the prognostic signature. Results In this study, we systemically portrayed the proteogenomic landscape of ferroptosis regulators in GBM. We observed that some mutation-specific ferroptosis regulators, such as down-regulated ACSL4 in EGFR-mutated patients and up-regulated FADS2 in IDH1-mutated patients, were linked to the inhibited ferroptosis activity in GBM. To interrogate the valuable treatment targets, we performed the survival analysis and identified five ferroptosis regulators (ACSL3, HSPB1, ELAVL1, IL33, and GPX4) as the prognostic biomarkers. We also validated their efficiency in external validation cohorts. Notably, we found overexpressed protein and phosphorylation abundances of HSPB1 were poor prognosis markers for overall survival of GBM to inhibit ferroptosis activity. Alternatively, HSPB1 showed a significant association with macrophage infiltration levels. Macrophage-secreted SPP1 could be a potential activator for HSPB1 in glioma cells. Finally, we recognized that ipatasertib, a novel pan-Akt inhibitor, could be a potential drug for suppressing HSPB1 phosphorylation, inducing ferroptosis of glioma cells. Conclusion In summary, our study characterized the proteogenomic landscape of ferroptosis regulators and identified that HSPB1 could be a candidate target for ferroptosis-inducing therapy strategy for GBM.

The cultivation of drip-irrigated rice has resulted in lower yields. However, the decrease in rice yield under drip irrigation and its relationship with the existing water and N regime have not been fully explained. Research and development of optimized water and N-management techniques are crucial for increasing rice yield under drip irrigation. In this study, two irrigation treatments were set: conventional drip irrigation (DIO) and drip irrigation with water stress (DIS). Each irrigation treatment contained four N rates: urea N 240 kg ha−1 (LN), urea N 300 kg ha−1 (MN), urea N 360 kg ha−1 (HN), and ammonium sulfate N 300 kg ha−1 (AN). The soil’s ammonium and nitrate contents were measured on the 2nd and 28th days after N application at panicle initiation stage. At anthesis, the aboveground and root biomass of rice were measured. In heading and maturity stage the N content of aboveground was measured and the yield, yield components, and NPFP were assessed at maturity stage. The results showed the following: (1) On the second day after N application, the contents of soil NO3−-N and NH4+-N in the 0–10 cm soil layer were highest for both the DIO and DIS. On the 28th day after N application, the soil NO3−-N content was highest at the 20–40 cm depth, while the soil NH4+-N content was still highest at the 0–10 cm depth. (2) The aboveground and root biomass in DIO treatment were significantly higher than in DIS. Furthermore, the root biomass at the 0–10 cm depth was significantly greater than at the 10–50 cm depth for both the DIO and DIS treatments. In the DIO treatment, the root biomass at the 10–50 cm depth was significantly higher with the HN and AN treatments compared to MN. However, in the DIS treatment, the root biomass at the 10–50 cm depth did not show significant differences between the MN, HN, and AN. (3) N accumulation in rice was significantly higher for the DIO treatment compared to the DIS treatment. Under the same irrigation treatment, the N accumulation in rice was highest in the AN and lowest in the LN. The PrNTA and PrNTC in DIS were significantly higher than in DIO, while the PoNAA and PoNAC were significantly lower in DIS. (4) The number of panicles, spikelets per panicle, seed-setting rate, 1000-grain weight, and grain yield were significantly lower in DIS. Under the DIS, these parameters were not significantly different among the MN, HN, and AN. In the DIO, the seed-setting rate, 1000-grain weight, and yield were not significantly different between the HN and AN, but were significantly higher than in the MN and LN. (5) NPFP was significantly higher in the DIO compared to the DIS. Among the different N rates, NPFP was highest with the AN treatment and lowest with the LN. In summary, under drip irrigation, there was a mismatch between soil mineral N and the distribution of rice roots, leading to reduced N accumulation and utilization in rice, ultimately impacting yield formation. Increasing N application and soil ammonium nutrition can improve rice yield under drip irrigation. However, optimizing N fertilizer management may not increase rice yield further when irrigation is further limited.

ObjectivesPatients with digestive diseases frequently suffer from dyspepsia and malabsorption, which may lead to muscle loss due to malnutrition. However, it is not clear whether digestive diseases are associated with sarcopenia. This study aims to explore the longitudinal association between digestive diseases and sarcopenia in middle-aged and older adults based on a nationally representative survey from China.MethodsWe used a prospective cohort study including 7,025 middle-aged and older adults aged ≥45 years from the 2011 to 2015 waves China Health and Retirement Longitudinal Study (CHARLS). Digestive diseases were identified using self-report. The assessment of sarcopenia was based on the Asian Working Group for Sarcopenia 2019 Consensus and included three components of muscle strength, physical performance, and muscle mass. Cox hazards regression was used to examine the association between digestive diseases and sarcopenia.ResultsThe prevalence of digestive diseases and the incidence of sarcopenia in middle-aged and older adults were 22.6% (95% CI = 21.6–23.6%) and 8.5% (95% CI = 7.8–9.1%). After adjusting for 15 covariates composed of three sets (demographic characteristics, lifestyles, and health status), digestive diseases were associated with a higher risk of sarcopenia (HR = 1.241, 95% CI = 1.034–1.490, P < 0.05). The associations were more pronounced among men, older adults aged 60–79, rural residents, and married people. In addition, the association between digestive diseases and sarcopenia was robust in the sensitivity analysis.ConclusionDigestive diseases were associated with an increased risk of sarcopenia in middle-aged and older adults aged ≥45 years. Early intervention of digestive diseases may help to reduce the incidence of sarcopenia in middle-aged and older adults.

BackgroundIncreasing evidence has shown that the intestinal microbiome (IM) is highly linked to colorectal cancer (CRC). To investigate scientific output, identify highly cited papers, and explore research hotspots and trends in the field of IM/CRC, we conducted a bibliometric and visualized analysis.MethodsA bibliographic search regarding IM/CRC research (2012-2021) was implemented on October 17, 2022. The terms attached to IM and CRC were searched for in the titles (TI), abstracts (AB), and author keywords (AK). The main information was extracted from the Web of Science Core Collection (WoSCC). Biblioshiny from R packages and VOSviewer were used for data visualization.ResultsA total of 1725 papers related to IM/CRC were retrieved. Publications on IM/CRC have grown rapidly from 2012 to 2021. China and the United States were in the leading position for publications in this field and made the most significant contributions to IM/CRC research. Shanghai Jiao Tong University and Harvard University were the most productive institutions. The high-yield authors were Yu Jun and Fang Jing Yuan. The International Journal of Molecular Sciences published the most papers, whereas Gut had the most citations. Historical citation analysis showed the evolution of IM/CRC research. Current status and hotspots were highlighted using keyword cluster analysis. The hot topics include the effect of IM on tumorigenesis, the effect of IM on CRC treatment, the role of IM in CRC screening, the mechanisms of IM involvement in CRC, and IM modulation for CRC management. Some topics, such as chemotherapy, immunotherapy, Fusobacterium nucleatum and short-chain fatty acids could be the focus of IM/CRC research in the coming years.ConclusionThis research evaluated the global scientific output of IM/CRC research and its quantitative features, identified some significant papers, and gathered information on the status and trends of IM/CRC research, which may shape future paths for academics and practitioners.

Abstract Autophagy has been demonstrated to play important roles in the infection and pathogenesis of many viruses. We previously found that porcine parvovirus (PPV) infection can induce autophagy in porcine placental trophoblast cells (PTCs), but its underlying mechanism has not yet been fully revealed. In this study, we showed that PPV infection inhibited the activation of mTORC1 and promoted the expression of Beclin 1 and LC3II in PTCs. Treatment with a mTOR activator inhibited the expression of Beclin 1 and LC3II, as well as autophagy formation, and reduced viral replication in PPV-infected PTCs. Furthermore, we found that inhibition of AMPK expression, but not the inhibition of PI3K/Akt, p53, or MAPK/ERK1/2 pathway activation, can significantly increase mTOR phosphorylation in PPV-infected PTCs. Then, we found that the regulation of mTOR phosphorylation by AMPK was mediated by Raptor. AMPK expression knockout inhibited the activation of Raptor, decreased the expression of Beclin 1 and LC3II, suppressed the formation of autophagosomes, and reduced viral replication during PPV infection. Together, our results showed that PPV infection induces autophagy to promote viral replication by inhibiting the activation of mTORC1 through activation of the AMPK/Raptor pathway. These findings provide information to understand the molecular mechanisms of PPV-induced autophagy.

The condition of the water in drinking water source environments is critical for public health. However, in recent years, the rapid growth of algae has become a significant threat to the safety of the drinking water supply. This study investigated the temporal trends and spatial differences in algae in the Nanwan Reservoir during 2022. Regression analysis using the least-squares method demonstrated that water temperature and initial biomass concentration were critical parameters that influenced the rate of algal growth. An air–water–algal growth model (AWAM) for algal growth prediction was developed using a 30-day forecast of air temperature, an air-to-water temperature extrapolating equation, a water temperature–algal growth relationship, and only four monthly measurements of algal concentration. The results demonstrated that the model accurately predicted algal growth in the next 30 days, with an R2 of 0.738, which aligned with the monitored results. Compared to the upstream Wudaohe River inflow point, algal growth in the drinking water intake area near the downstream dam was delayed by at least 30 days. By using the upstream inflow area as a reference point, the prediction period was extended to provide a 60-day early warning. The extended prediction period and the reduced need for monitoring data make the model more convenient for guiding the prevention and control of algal blooms in drinking water reservoirs.

Glioma is a malignant tumor with a high fatality rate, originating in the central nervous system. Even after standard treatment, the prognosis remains unsatisfactory, probably due to the lack of effective therapeutic targets. The family of transmembrane proteins (TMEM) is a large family of genes that encode proteins closely related to the malicious behavior of tumors. Thus, it is necessary to explore the molecular and clinical characteristics of newly identified oncogenes, such as transmembrane protein 60 (TMEM60), to develop effective treating options for glioma. We used bioinformatic methods and basic experiments to verify the expression of transmembrane protein 60 in gliomas and its relationship with 1p and 19q (1p19q) status, isocitrate dehydrogenase (IDH) status, patient prognosis, and immune cell infiltration using public databases and clinical samples. In addition, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to detect co-expressed genes. Thus, we inhibited the expression of transmembrane protein 60 to observe the proliferation and activity of glioma LN229 cells. We found transmembrane protein 60 was significantly upregulated in glioma compared with that in normal brain tissue at the mRNA. In the subgroups of World Health Organization high grade, isocitrate dehydrogenase wildtype, 1p and 19q non-codeletion, or isocitrate dehydrogenase wild combined with 1p and 19q non-codeletion, the expression of transmembrane protein 60 increased, and the prognosis of glioma patients worsened. In the transmembrane protein 60 high expression group, infiltration of immune cells and stromal cells in the tumor microenvironment increased, tumor purity decreased, and immune cells and pathways were activated. The immune cells mainly included regulatory T-cell, gamma delta T-cell, macrophages M0, neutrophils, and CD8+ T-cells. Overexpression of co-inhibitory receptors (CTLA4, PDL1 and CD96) may promote the increase of depletion of T-cell, thus losing the anti-tumor function in the transmembrane protein 60 high expression group. Finally, we found that transmembrane protein 60 silencing weakened the viability, proliferation, and colony formation of glioma LN229 cells. This is the 0 report on the abnormally high expression of transmembrane protein 60 in glioma and its related clinical features, such as tumor microenvironment, immune response, tumor heterogeneity, and patient prognosis. We also found that transmembrane protein 60 silencing weakened the proliferation and colony formation of glioma LN229 cells. Thus, the new oncogene transmembrane protein 60 might be an effective therapeutic target for the clinical treatment of glioma.

ObjectivesThe aim of this study was to establish and validate a MRI-based radiomics nomogram to predict progression-free survival (PFS) of clival chordoma.MethodsA total of 174 patients were enrolled in the study (train cohort: 121 cases, test cohort: 53 cases). Radiomic features were extracted from multiparametric MRIs. Intraclass correlation coefficient analysis and a Lasso and Elastic-Net regularized generalized linear model were used for feature selection. Then, a nomogram was established via univariate and multivariate Cox regression analysis in the train cohort. The performance of this nomogram was assessed by area under curve (AUC) and calibration curve.ResultsA total of 3318 radiomic features were extracted from each patient, of which 2563 radiomic features were stable features. After feature selection, seven radiomic features were selected. Cox regression analysis revealed that 2 clinical factors (degree of resection, and presence or absence of primary chordoma) and 4 radiomic features were independent prognostic factors. The AUC of the established nomogram was 0.747, 0.807, and 0.904 for PFS prediction at 1, 3, and 5 years in the train cohort, respectively, compared with 0.582, 0.852, and 0.914 in the test cohort. Calibration and risk score stratified survival curves were satisfactory in the train and test cohort.ConclusionsThe presented nomogram demonstrated a favorable predictive accuracy of PFS, which provided a novel tool to predict prognosis and risk stratification. Our results suggest that radiomic analysis can effectively help neurosurgeons perform individualized evaluations of patients with clival chordomas.

A refractive index (RI) sensor based on perfluorinated plastic optical fiber (PF-POF) is introduced in this paper. The PF-POF as multi-mode fiber was side-polished (SP) to form a macro-bending single-mode-multimode-single-mode (SMS) structure. Both ends of the sensor were closely connected to single-mode quartz optical fiber (SMF). The spectral characteristics of the sensor are measured, analyzed and discussed. The results show that when the length of PF-POF is 8 cm, the macro-bending radius is 3 cm, and the SP-depth is 20 μm. The intensity sensitivity reaches −219.504 dBm/RIU in the range of RI = 1.330 ~ 1.356. A reference is provided for the application of PF-POF in RI sensor in the future. The sensor is featured with low-cost, good flexibility and high efficiency.

BackgroundHelicobacter pylori (H. pylori) infects half of the human population globally. Eradication rates with triple or quadruple therapy have decreased owing to the increasing rate of antibiotic resistance. Jinghua Weikang capsule (JWC) is the first and most popular Chinese patent medicine approved by the state for the treatment of gastritis and peptic ulcers caused by H. pylori infection in China. Previous studies have found that JWC has a certain bactericidal effect on drug-resistant H. pylori and its major component, Chenopodium ambrosioides L. inhibits biofilm formation, but the mechanism remains unclear. This study focused on drug-resistant H. pylori and explored whether JWC could reverse drug resistance and its related mechanisms.MethodThe agar plate dilution method, E-test method, and killing kinetics assay were used to evaluate the bactericidal effect of JWC on antibiotic-resistant H. pylori and its effect on antibiotic resistance. Sanger sequencing was used to detect mutations in drug resistance genes. The crystal violet method, scanning electron microscopy, and confocal laser scanning microscopy were used to evaluate the effects of JWC on biofilms. qPCR was performed to evaluate the effect of JWC on the expression of efflux pump-related genes. qPCR and immunofluorescence were used to evaluate the effects of JWC on H. pylori adhesion.ResultsJWC showed considerable antibacterial activity against drug-resistant H. pylori strains, with minimum inhibitory concentration (MIC) values ranging from 64 to 1,024 μg/ml. The MIC of metronidazole (MTZ) against H. pylori 26,695–16R decreased from 64 to 6 μg/ml after treatment with 1/2 MIC of JWC. The resistance of H. pylori 26,695–16R to MTZ was reversed by JWC, and its effect was better than that of PaβN and CCCP. H. pylori 26,695–16R is a moderate biofilm-forming strain, and JWC (16–64 μg/ml) can inhibit the formation of biofilms in H. pylori 26,695–16R. JWC reduced the expression of HP0605-HP0607 (hefABC), HP0971-HP0969 (hefDEF), HP1327-HP1329 (hefGHI), and HP1489-HP1487. JWC reduced the adhesion of H. pylori to GES-1 cells and the expression of adhesives NapA, SabA, and BabA.ConclusionThe reversal of MTZ resistance by JWC may be achieved through the adhesin/efflux pump-biofilm pathway.

High‐resolution temperature measurement is nerve‐wracking obstruction for precise characterization of many physical, chemical, and biological processes. To solve this problem, a novel microcavity–optomechanical–oscillation‐based thermometer is proposed. The microcavity serving as a link parametrically couples the mechanical resonator and optical resonator in the same structure and provides a natural and highly sensitive temperature transduction mechanism and ultrahigh‐resolution optical demodulation. The mathematical model of geometrical parameters, mechanics, and material properties for temperature response mechanism is established and verified experimentally. The proposed thermometer has a thermal sensitivity of 11 300 Hz °C−1 and an ultrahigh‐temperature resolution of 1 × 10−4 °C, to the best of one's knowledge, which is the highest temperature resolution with a silica cavity.

BackgroundSeveral studies have shown that neurodegenerative diseases (e.g., Parkinson’s disease [PD] and Alzheimer’s disease [AD]) are associated with inflammatory bowel disease (IBD), but the causality and direction of their associations remain unclear. Mendelian randomization (MR) studies have explored the causal effects of IBD on PD and AD. However, only a few studies examined this reverse association. Thus, this study aimed to explore whether there are causal associations of genetically predicted PD and AD with IBD, using a two-sample MR study.MethodsSummary statistics for IBD, ulcerative colitis (UC), and Crohn’s disease (CD) were derived from a genome-wide association study (GWAS) meta-analysis, which included the International IBD Genetics Consortium and the UK IBD Genetics Consortium (n=59,957). Genetic variants associated with the largest meta-analysis of GWAS of PD (n=1,474,097) and AD (n=455,258) were used as instrumental variables. We used multiple methods, including inverse variance weighted (IVW), weighted median (WM), MR-Egger regression, weighted mode, and Robust Adjusted Profile Score (RAPS) methods, to estimate the effects of genetically predicted PD and AD on IBD. To confirm the validity of the analysis, we also evaluated the pleiotropic effects, heterogeneity, and leave-one-out sensitivity analysis that drive causal associations.ResultsThe results of the IVW method, WM, and RAPS showed that genetically predicted PD was significantly associated with an increased risk of UC (odds ratio [OR]IVW=1.068, ORWM=1.107, ORRAPS=1.069, all P0.05), or statistical heterogeneity. The sensitivity analysis results of the leave-one-out sensitivity analysis showed that the causal effect estimations of genetically predicted PD and AD on IBD were robust.ConclusionsOur MR study corroborated a causal association between genetically predicted PD and IBD but did not support a causal effect of genetically predicted AD on IBD. More animal experiments or population-based observational studies are required to clarify the underlying mechanisms of PD and IBD.

5G industry wireless private network has become one of the most important services for operators enabling the vertical industry.The gradual maturity of virtualization, multi-access edge computing, network slicing and other technologies have provided a key technical guarantee for the development of 5G industry wireless private network.The development status, key technology, requirement model, challenges and development recommendations of 5G industry wireless private network were introduced.The concept and classification of 5G industry wireless private network were introduced, the key technologies were generalized, and the design ideas and the main reference index were put forward aiming at business requirement.Finally, the challenges faced by operators when developing 5G wireless private network services were summarized and development suggestions were given.

The application and development of 5G in the field of cement mining has drawn attention.Cement factories, usually built around limestone mines, in which 5G network shows great value to be used for safe inspection by drones, UAV 3D modeling of mines, unmanned driving and remote control of mining vehicles, of excavators and other mobile services.Firstly, the main business scenarios and network requirements of 5G in cement industry mines was analyzed.Secondly, via the analysis of practical cases and test data of 5G cement mine network deployment, the difficulty of 5G network coverage in mines lies in the poor coverage rate and signal strength of 5G network in limestone mines, which are due to mountain shielding effect mined by hillside terraced fields.Finally, the difficulties of 5G mine network coverage were summarized from the aspects of supporting facilities, planning simulation and environmental dynamic change, and preliminary suggestions were given correspondingly.

Abstract Porcine Parvovirus (PPV), a pathogen causing porcine reproductive disorders, encodes two capsid proteins (VP1 and VP2) and three nonstructural proteins (NS1, NS2 and SAT) in infected cells. The PPV NS2 mRNA is from NS1 mRNA after alternative splicing, yet the corresponding mechanism is unclear. In this study, we identified a PPV NS1 mRNA binding protein SYNCRIP, which belongs to the hnRNP family and has been identified to be involved in host pre-mRNA splicing by RNA-pulldown and mass spectrometry approaches. SYNCRIP was found to be significantly up-regulated by PPV infection in vivo and in vitro. We confirmed that it directly interacts with PPV NS1 mRNA and is co-localized at the cytoplasm in PPV-infected cells. Overexpression of SYNCRIP significantly reduced the NS1 mRNA and protein levels, whereas deletion of SYNCRIP significantly reduced NS2 mRNA and protein levels and the ratio of NS2 to NS1, and further impaired replication of the PPV. Furthermore, we found that SYNCRIP was able to bind the 3′-terminal site of NS1 mRNA to promote the cleavage of NS1 mRNA into NS2 mRNA. Taken together, the results presented here demonstrate that SYNCRIP is a critical molecule in the alternative splicing process of PPV mRNA, while revealing a novel function for this protein and providing a potential target of antiviral intervention for the control of porcine parvovirus disease.

Traumatic brain injury (TBI) is a subset of brain injury induced by external mechanical forces to the head or neck. TBI has been reported to be one of the leading causes of disability, and it causes a huge financial burden around the world. Aloin is the major anthraquinone glycoside extracted from Aloe species, and has presented anti-tumour, anti-oxidative and anti-inflammatory activities. However, few studies have focused the effect of aloin in treatment of TBI. Nicotinamide adenine dinucleotide phosphate oxidase (NOX) is the only subset of enzymes which produces solely the reactive oxygen species (ROS). A recent study showed that activation of NOX might aggravate the primary TBI, and among these members, NOX2 is the key member in regulation of uncontrolled ROS expression, and thus plays a critical role in development of inflammatory diseases. Here, we noticed that inhibition of NOX2 combined with aloin treatment promoted the recovery of brain function in a mice model as well as the viability rate in a cell model. A further study found that the inflammation response process was also inhibited after treatment. Then, we found that these effects might be mediated by the PI3K/AKT/mTOR signalling pathway and NOX2 might be a therapeutic target for TBI.

Fabry-Perot (F-P) filter based fiber Bragg grating (FBG) demodulation has been widely applied in many areas. However, the F-P filter suffers from random fluctuation in wavelength scanning when the ambient temperature changes severely, which reduces the demodulation accuracy inevitably. In this paper, a demodulation method based on an etalon and a fiber-optic Michelson interferometer is proposed to improve the demodulation accuracy in an environment with variable temperature. The experiment is carried out by constructing an interferometer with long-optical path difference (OPD). When the environment temperature increases from 20 °C to 60 °C in 30 minutes, the results show that the error of the proposed method is reduced from ± 20.39 pm to ± 6.89 pm. The standard deviation is 3.11 pm, which is reduced by 43.92% compared with the etalon-based demodulation method.

In this paper, we propose and demonstrate the optical fiber Fabry-Perot (F-P) interferometer based on birefringent crystals and polarization technology. For recovering the variation signals, the orthogonal signals are obtained based on the birefringent crystal characteristics and the proposal thickness difference between the two crystals. The differential cross multiplication (DCM) algorithm is utilized to demodulate the orthogonal signals to obtain the phase variation of the optical fiber F-P sensor. The proposed interferometer has a minimum detection phase of 0.014 rad/ √Hz at the frequency of 25 kHz. In the experiment, two kinds of vibration signals with frequencies of 25 kHz and 15 kHz are used and the proposed interferometer SNR is 70 dB and 75 dB under the corresponding reference environment respectively. The experiment results show that the proposed interferometer can realize the measurement of large dynamic signals and has high stability. The proposed interferometer has the advantages of fast demodulation and good environmental adaptability.

A demodulation method of spatial domain based on low-coherence interferometry is proposed. The original signal of spatial domain acquired by linear CCD is filtered with all-phase filter to obtain the fringe pattern, and the phase signal of the spatial frequency domain is retained after filtering. The fringe pattern analysis is employed to avoid phase ambiguity and realize large measurement range in demodulation. Afterwards, using the centroid position of the fringe, the peak position of a single fringe pattern can be obtained, and finally the accurate peak position is calculated with seven-step phase-shifting interferometry. The demodulation method is demonstrated experimentally with Fabry-Perot sensing system. At room temperature, it is proved that the full-scale measurement error is less than 0.019% in the range of 11 kPa to 290 kPa. From -20 °C to 70 °C, the accuracy is stable and the maximum error is less than 0.054 kPa. The demodulation method has the potential to enhance the measurement accuracy and adaptability in actual environment for universal applications.

Hydrogen cyanide (HCN) gas based fiber Bragg grating (FBG) interrogation is a promising approach in thermal cycling environment. However, the HCN spectrum suffers from strong noise and baseline wander induced by the interrogation system, which limits the measurement stability and reliability. In this paper, we propose an integrated method for spectral denoising and baseline removal to improve the interrogation performance. The method combines the classical empirical mode decomposition (EMD) with the recently developed convolution window (CW) filtering. After the HCN spectrum is decomposed by EMD, the noise and baseline components are extracted from the intrinsic mode functions via a “multiband” filtering approach. Verification experiments are performed using HCN gas cells with different absorption spectra. The results show that the proposed method is able to remove both noise and baseline with minimum signal distortion. Compared with other methods, the optimal interrogation stability with 25 Torr HCN gas cell is improved from ±1.43 pm to ±0.87 pm. For the 100 Torr HCN gas cell, the optimal interrogation stability is improved from ±1.75 pm to ±0.99 pm. In addition, the proposed method also improves the reliability of the interrogation system in the case of large light attenuation.

Calibration by hydrogen cyanide (HCN) absorption spectrum can make the fiber Bragg grating (FBG) demodulation system immune to environment temperature changes. The transmittance function of Fabry-Perot (F-P) filter will result in the distortion of the HCN absorption peaks. A demodulation scheme based on deconvolution is proposed to eliminate the impact of the F-P filter. A recovered HCN absorption spectrum with deeper, narrower and more symmetrical absorption peaks is obtained for the Bragg wavelength calibration. It is proved experimentally that this method can reduce the standard deviation of demodulated FBG wavelengths by at least 45.9% at a constant temperature, and 42.9% in the environment of varying temperature from 10 °C to 60 °C. Compared with the method of Bragg wavelength calibration based on the original HCN absorption spectrum, the spectral bandwidth that can be accurately demodulated is also expanded to the whole C-band.

Breast cancer (BC) is one of the most common malignant tumors found in females. Previous studies have demonstrated that curcumin, which is a type of polyphenol compound extracted from Curcuma longa underground rhizome, is able to inhibit the survival of cancer cells. However, the functional role and mechanism of curcumin in BC are still unclear. The Cell Counting Kit-8 assay was performed to examine the effects of curcumin on cell viability in the BC cell lines MDA-MB-453 and MCF-7. The levels of lipid reactive oxygen species (ROS), malondialdehyde (MDA) production, and intracellular Fe2+ were determined to assess the effects of curcumin on cell ferroptosis. Western blot analysis was also carried out to detect the protein levels. Finally, the antitumorigenic effect of curcumin on BC was identified in a xenograft tumor model. In the present study, the results indicated that curcumin could dose-dependently suppress the viability of both MDA-MB-453 and MCF-7 cells. Further studies revealed that curcumin facilitated solute carrier family 1 member 5 (SLC1A5)-mediated ferroptosis in both MDA-MB-453 and MCF-7 cells by enhancing lipid ROS levels, lipid peroxidation end-product MDA accumulation, and intracellular Fe2+ levels. In vivo experiments demonstrated that curcumin could significantly hamper tumor growth. Collectively, the results demonstrated that curcumin exhibited antitumorigenic activity in BC by promoting SLC1A5-mediated ferroptosis, which suggests its use as a potential therapeutic agent for the treatment of BC.

In this study, the responses of Dissolved Air Flotation (DAF), sedimentation, and sand filtration treatment processes on feed water with varied algal concentrations were investigated, based on a technical–economic analysis using data collected from a drinking water treatment plant (DWTP) in Guangxi, China. Cost-effective drinking water treatment processes for water sources with varied algae concentrations were proposed. The results showed that DAF was able to achieve almost 95% removal efficiency, while sedimentation was only able to reach 90% under different Polyaluminum Chloride (PACl)/dry cell weight concentrations in the DWTP. When algae concentrations increase, switching from sedimentation to DAF reduces treatment costs as DAF is more efficient for algae removal, which extends the backwashing interval of sand filtration. The threshold of sedimentation/DAF switching also depends on the quality requirement of the treated water. The lower the algae concentration in the treated water, the earlier the switch should be made from sedimentation to DAF. For instance, when the effluent thresholds are 1.2 mg·L−1, 0.8 mg·L−1, or 0.4 mg·L−1, DAF should be adopted instead of sedimentation—at feed algae concentrations of 43.9 mg·L−1, 31.5 mg·L−1, and 17.3 mg·L−1, respectively, in the raw water. The results set a baseline for a cost-effective drinking water treatment strategy based on a techno-economic model, which can precisely control the coagulation dosage and backwash interval of sand filtration coupled with sedimentation/DAF switching in algae-laden raw water.

Breast cancer is a common malignant tumor with a high incidence of recurrence and metastasis. It has been reported that propofol has certain anti-breast cancer effects, but the intrinsic molecular mechanism remains unclear. This study investigated the effect of propofol on breast cancer MCF-7 cells and its possible regulatory mechanisms. MCF-7 cells were treated by propofol, and then the effects of propofol on cell growth and epithelial-mesenchymal transition (EMT) were studied. We subsequently testified whether miR-21 was a downstream effector of propofol. As a result, propofol repressed the proliferation and migration of MCF-7 cells, but significantly induced apoptosis. Meanwhile, miR-21 expression and EMT were inhibited by propofol stimulation. The effects of propofol on MCF-7 cells proliferation, apoptosis and EMT were all attenuated when miR-21 was overexpressed. Besides this, the activation of PI3K/AKT and Wnt3a/β-catenin pathways was reduced by propofol stimulation in a miR-21-depedent manner. In conclusion, propofol can inhibit the proliferation and EMT of MCF-7 cells by down-regulating miR-21 expression. Moreover, miR-21 can further regulate PI3K/AKT and Wnt/β-catenin pathways.

Helicobacter pylori (H. pylori) has so far infected more than half the global population. It is the most important and controllable risk factor for gastric cancer. The elderly, who are at a higher incidence of the infection, are also commonly found to develop antibiotic resistance. The symptoms, diagnosis, clinical features (of gastric or extra-digestive diseases), and treatment of H. pylori infection in the elderly, are different from that in the non-elderly. Health conditions, including comorbidities and combined medication have limited the use of regular therapies in elderly patients. However, they can still benefit from eradication therapy, thus preventing gastric mucosal lesions and gastric cancer. In addition, new approaches, such as dual therapy and complementary therapy, have the potential to treat older patients with H. pylori infection.

We proposed and demonstrated an optical fiber Fabry-Perot (F-P) sensor with long sensing length for strain measurement. The structure of the sensor was fabricated with a simple process by splicing a long single mode fiber (SMF) with a short section of hollow-core fiber (HCF). The end of the SMF was cleaved with smooth reflective surface which hence allows the sensing length of the proposed sensor can achieve several centimeters with good visibility. Sensors with different sensing lengths were fabricated and experimentally analyzed in this paper. The relationship between the optical path difference (OPD) of the sensor and strain is found to be linear. With the sensing length of ~10 mm and ~49 mm, the sensor can achieve the OPD strain sensitivity of 20.42 nm/με and 115.57 nm/με at 1550 nm over the range of 1000 με respectively. Besides, with the sensing length of ~10 mm, the OPD temperature sensitivity of the sensor was 187.64 nm/°C. With the advantages of easy making, low cost and long sensing length, it is anticipated that the proposed sensor has great potential in measuring the strain with a large distribution.

A highly sensitive temperature sensor based on hollow microsphere is demonstrated for ocean temperature measurements. The effect of sensor parameters on temperature sensitivity is investigated with the experiments using different parameters hollow microspheres, tapered fibers with different waist diameters and different kinds of solution. A high sensitivity of 396.70 pm/°C is obtained when the hollow microsphere is filled with ethanol. A high temperature resolution 1.75 × 10-3 °C is obtained when the hollow microsphere is filled with cyclohexane. The volatilization process of 2 μL ethanol is detected to demonstrate good dynamical response of the sensor.

A compact fiber optic Fabry-Perot (F-P) sensor for simultaneous acoustic and temperature measurement has been proposed in the research. The sensor consists of silicon-glass-silicon sandwich structure by anodic bonding. The air F-P cavity with a circular hole and slots utilizes the deformation of the silicon membrane to realize acoustic measurement, and the silicon F-P cavity utilizes the temperature sensitivity of the refractive index of silicon to realize temperature measurement. The acoustic signal and temperature information could be recovered by acoustic and temperature demodulation method, respectively. The experimental results demonstrate that the sensor has a signal-to-noise ratio (SNR) of 53 dB and the acoustic sensitivity of 4.65 mV/Pa at 21 kHz, and the temperature sensitivity of 123.10 nm/°C at 20 °C~120 °C. The proposed fiber optic F-P sensor has great potential for simultaneous acoustic and temperature measurement in aviation, due to its compact structure, mass production and immunity to electromagnetic interference.

Quantitative detection of cardiac troponin biomarkers in blood is an important method for clinical diagnosis of acute myocardial infarction (AMI). In this work, a whispering gallery mode (WGM) microcavity immunosensor based on a prefab hollow glass microsphere (HGMS) with liquid crystal (LC) sensitization was proposed and experimentally demonstrated for label-free cardiac troponin I-C (cTnI-C) complex detection. The proposed fiber-optic immunosensor has a simple structure; the tiny modified HGMS serves as the key sensing element and the microsample reservoir simultaneously. A sensitive LC layer with cTnI-C recognition ability was deposited on the inner wall of the HGMS microcavity. The arrangement of LC molecules is affected by the cTnI-C antigen—antibody binding in the HGMS, and the small change of the surface refractive index caused by the binding can be amplified owing to the birefringence property of LC. Using the annular waveguide of the HGMS, the WGMs were easily excited by the coupling scanning laser with a microfiber, and an all-fiber cTnI-C immunosensor can be achieved by measuring the resonant wavelength shift of the WGM spectrum. Moreover, the dynamic processes of the cTnI-C antigen—antibody binding and unbinding was revealed by monitoring the wavelength shift continuously. The proposed immunosensor with a spherical microcavity can be a cost-effective tool for AMI diagnosis.

Abstract Background Botryococcus braunii is known for its high hydrocarbon content, thus making it a strong candidate feedstock for biofuel production. Previous study has revealed that a high cobalt concentration can promote hydrocarbon synthesis and it has little effect on growth of B. braunii cells. However, mechanisms beyond the cobalt enrichment remain unknown. This study seeks to explore the physiological and transcriptional response and the metabolic pathways involved in cobalt-induced hydrocarbon synthesis in algae cells. Results Growth curves were similar at either normal or high cobalt concentration (4.5 mg/L), suggesting the absence of obvious deleterious effects on growth introduced by cobalt. Photosynthesis indicators (decline in Fv/Fm ratio and chlorophyll content) and reactive oxygen species parameters revealed an increase in physiological stress in the high cobalt concentration. Moreover, cobalt enrichment treatment resulted in higher crude hydrocarbon content (51.3% on day 8) compared with the control (43.4% on day 8) throughout the experiment (with 18.2% improvement finally). Through the de novo assembly and functional annotation of the B. braunii race A SAG 807-1 transcriptome, we retrieved 196,276 non-redundant unigenes with an average length of 1086 bp. Of the assembled unigenes, 89,654 (45.7%), 42,209 (21.5%), and 32,318 (16.5%) were found to be associated with at least one KOG, GO, or KEGG ortholog function. In the early treatment (day 2), the most strongly upregulated genes were those involved in the fatty acid biosynthesis and metabolism and oxidative phosphorylation, whereas the most downregulated genes were those involved in carbohydrate metabolism and photosynthesis. Genes that produce terpenoid liquid hydrocarbons were also well identified and annotated, and 21 (or 29.2%) were differentially expressed along the cobalt treatment. Conclusions Botryococcus braunii SAG 807-1 can tolerate high cobalt concentration and benefit from hydrocarbon accumulation. The time-course expression profiles for fatty acid biosynthesis, metabolism, and TAG assembly were obtained through different approaches but had equally satisfactory results with the redirection of free long-chain fatty acid and VLCFA away from TAG assembly and oxidation. These molecules served as precursors and backbone supply for the fatty acid-derived hydrocarbon accumulation. These findings provide a foundation for exploiting the regulation mechanisms in B. braunii race A for improved photosynthetic production of hydrocarbons.

Abstract A complete understanding and good adherence are crucial for successful Helicobacter pylori eradication. Proper frequency of reminders might be helpful to both doctors and patients to maintain adherence during treatment. The study was to evaluate the influence of an intensive follow‐up system based on a clinical database on H. pylori eradication therapy. A total of 196 eligible patients were equally and randomly divided into an intensive follow‐up group and a control group. Both groups were administered bismuth‐containing quadruple therapy for 14 days. Patients in the intensive follow‐up group were informed of pre‐treatment, including the duration and potential adverse events. Subsequently, they received telephone follow‐ups on days 3 and 14 and 3 days before the urea breath test (UBT). The time points were automatically reminded by a follow‐up system in the established clinical database. The control group was only informed of pre‐treatment information. UBT was performed 4 weeks after treatment in both groups to assess the presence of H. pylori. The eradication rate, patient compliance, and adverse events were calculated and compared. The H. pylori eradication rates of the intensive follow‐up and control groups were 94.7% (90/95, 95% CI: 90%–99%) and 92.9% (78/84, 95% CI: 87%–98%), respectively, by PP analysis (p = 0.601), and 91.8% (90/98, 95% CI: 86%–97%) and 81.6% (80/98, 95% CI: 74%–89%) by ITT analysis (p = 0.035). Adverse events occurred in 9 intensive follow‐up group patients and 12 in the control group. Adherence was 96.9% (95/98) in the intensive follow‐up group and 85.7% (84/98) in the control group. Semi‐automatic intensive follow‐up contributed to a higher eradication rate and adherence to H. pylori treatment.

The present study investigated the stability and efficacy of a biosurfactant produced by Klebsiella sp. KOD36 under extreme conditions and its potential for enhancing the solubilization and degradation of phenanthrene in various environmental matrices. Klebsiella sp. KOD36 produced a mono-rhamnolipids biosurfactant with a low critical micelle concentration (CMC) value. The biosurfactant was stable under extreme conditions (60 °C, pH 10 and 10% salinity) and could lower surface tension by 30% and maintained an emulsification index of > 40%. The emulsion index was also higher (17–43%) in the presence of petroleum hydrocarbons compared to synthetic surfactant Triton X-100. Investigation on phenanthrene degradation in three different environmental matrices (aqueous, soil-slurry and soil) confirmed that the biosurfactant enhanced the solubilization and biodegradation of phenanthrene in all matrices. The high functional stability and performance of the biosurfactant under extreme conditions on phenanthrene degradation show the great potential of the biosurfactant for remediation applications under harsh environmental conditions.

BackgroundMore than 30–40% of uric acid is excreted via the intestine, and the dysfunction of intestinal epithelium disrupts uric acid excretion. The involvement of gut microbiota in hyperuricemia has been reported in previous studies, but the changes and mechanisms of intestinal immunity in hyperuricemia are still unknown.MethodsThis study developed a urate oxidase (Uox)-knockout (Uox–/–) mouse model for hyperuricemia using CRISPR/Cas9 technology. The lipometabolism was assessed by measuring changes in biochemical indicators. Furthermore, 4-kDa fluorescein isothiocyanate–labeled dextran was used to assess gut barrier function. Also, 16S rRNA sequencing was performed to examine the changes in gut microbiota in mouse feces. RNA sequencing, Western blot, Q-PCR, ELISA, and immunohistochemical analysis were used for measuring gene transcription, the number of immune cells, and the levels of cytokines in intestinal tissues, serum, kidney, liver, pancreas, and vascellum.ResultsThis study showed that the abundance of inflammation-related microbiota increased in hyperuricemic mice. The microbial pattern recognition–associated Toll-like receptor pathway and inflammation-associated TNF and NF-kappa B signaling pathways were significantly enriched. The increased abundance of inflammation-related microbiota resulted in immune disorders and intestinal barrier dysfunction by upregulating TLR2/4/5 and promoting the release of IL-1β and TNF-α. The levels of epithelial tight junction proteins occludin and claudin-1 decreased. The expression of the pro-apoptotic gene Bax increased. The levels of LPS and TNF-α in systemic circulation increased in hyperuricemic mice. A positive correlation was observed between the increase in intestinal permeability and serum levels of uric acid.ConclusionHyperuricemia was characterized by dysregulated intestinal immunity, compromised intestinal barrier, and systemic inflammation. These findings might serve as a basis for future novel therapeutic interventions for hyperuricemia.

Growing evidence has demonstrated the influence of internal nitrogen (N) and phosphorus (P) on harmful algae blooms in eutrophic freshwater ecosystems. However, the main controlling factors for internal N and P release risks, and whether these factors vary as environmental conditions change, remains poorly understood. We evaluated potential release risks of N and P from sediments in two freshwater reservoirs in Beihai City, southern China, by evaluating apparent nutrient fluxes during simulated static incubation experiments at two temperatures (15 °C and 25 °C). Sediments were analyzed to determine their basic properties as well as N and P fractions. Results showed that the main controlling factors of the apparent fluxes in dissolved total P, soluble reactive P, total N, and ammonium were related to sediment adsorption properties, redox properties, and microbial-mediated properties (e.g., water-extractable P, total inorganic N, redox-sensitive P, total organic carbon, organic P). The primary controlling factors for apparent N and P fluxes were dependent on the form of N and P and changed with temperature. The results suggest that care should be taken when simply using total N and P contents in sediments to evaluate their internal nutrient release risks.

A novel method realized by an adjustable line scanning polarized low-coherence interferometer is proposed and experimentally demonstrated to simultaneously measure pressure and temperature. The combination of birefringence wedge and compensation plate acts as an adjustable line scanning part to adapt various sensors by changing the thickness of compensation plate. The variations of pressure and temperature manifest as shift of the corresponding interference fringes on an infrared line array CCD. The dual-light-source is employed to fix the over occupation of CCD caused by an SLD with longer coherence length than an LED and enlarge the measuring range. An experiment of pressure and temperature was carried out to verify the effectiveness of the proposed method. The experimental results show that the proposed method is appropriate for the simultaneous measurement of pressure and temperature, and the sensor has a good linearity in pressure range of 3-228 kPa and temperature range of -20 °C -70 °C. The measurement errors of the pressure and temperature are less than 0.3%F.S. and 1.1%F.S., respectively.

Contamination of wastewater with organic-limited nitrates has become an urgent problem in wastewater treatment. The cooperating heterotrophic with sulfur autotrophic denitrification is an alternative process and the efficiency has been assessed in many studies treating simulated wastewater under different operating conditions. However, due to the complex and diverse nature of actual wastewater, more studies treating actual wastewater are still needed to evaluate the feasibility of collaborative denitrification. In this study, lab-scale experiments were performed with actual nitrate polluted water of two different concentrations, with glucose and sodium thiosulfate introduced as mixed electron donors in the coupling sulfur-based autotrophic and heterotrophic denitrification. Results showed that the optimum denitrification performance was exhibited when the influent substrate mass ratio of C/N/S was 1.3/1/1.9, with a maximum denitrification rate of 3.52 kg NO3−-N/(m3 day) and nitrate removal efficiency of 93% in the coupled systems. Illumina high-throughput sequencing analysis revealed that autotrophic, facultative, and heterotrophic bacteria jointly contributed to high nitrogen removal efficiency. The autotrophic denitrification maintained as the predominant process, while the second most prevalent denitrification process gradually changed from heterotrophic to facultative with the increase of influent concentration at optimum C/N/S ratio conditions. Furthermore, the initiation of dissimilatory nitrate reduction to ammonium (DNRA) was very pivotal in promoting the entire denitrification process. These results suggested that sulfur-based autotrophic coupled with heterotrophic denitrifying process is an alternative and promising method to treat nitrate containing wastewater.

A magnetic field sensor based on the magnetic-field-induced nanoparticle assembly effect in microcapillary whispering gallery mode (WGM) ring resonator is proposed and experimentally demonstrated. The chemical characteristics of nanoparticles and the silica microcapillary are used to link up the surface density of the resonator and the magnetic field intensity. The magnetic field variation changes the surface density of nanoparticles adsorbed on the sensor surface and respond to the WGM transmission spectra shift. Because of the powerful surface sensing capability of WGM, the maximum sensing sensitivity reaches 57.59 nm/mT and the detection limit reaches 1.39 × 10 –4, respectively. The magnetic field response characteristic of the sensor is studied as well. This provides the potential to fiber-based magnetic field sensing applications.

Myocardial infarction (MI) is a common presentation for ischemic heart disease, which is a leading cause of death. Emodin is a Chinese herbal anthraquinone used in several diseases. However, the effect of emodin in hypoxia-induced injury in cardiomyocytes has not been clearly elucidated. Our study aimed to clarify the functions of emodin in hypoxia-induced injury in rat cardiomyocytes H9c2 and explore the underlying mechanism. The effects of emodin on cell viability and apoptosis were analyzed by the Cell counting kit-8 assay and flow cytometry assay, respectively. The cell proliferation- and cell apoptosis-related proteins were detected by western blot. qRT-PCR was used to determine the relative expression of miR-138. Cell transfection was performed to alter miR-138 and MLK3 expression. miR-138 target was performed by dual luciferase activity assay. Sirt1/AKT and Wnt/β-catenin pathways-related factors phosphorylation were analyzed by western blot. Emodin inhibited hypoxia-induced injury in H9c2 cells by promoting cell viability and reducing cell apoptosis. miR-138 was down-regulated by hypoxia treatment but up-regulated by emodin. Up-regulation of miR-138 alleviated hypoxia-induced cell injury. Down-regulation of miR-138 attenuated the growth-promoting effect of emodin on hypoxia-induced injury, whereas up-regulation of miR-138 enhanced the growth-promoting effects of emodin. The underlying mechanism might be by inactivating Sirt1/AKT and Wnt/β-catenin pathways. MLK3 was negatively regulated by miR-138 expression and inactivated Sirt1/AKT and Wnt/β-catenin pathways. Emodin alleviated hypoxia-induced injury in H9c2 cells via up-regulation of miR-138 modulated by MLK3, as well as by activating Sirt1/AKT and Wnt/β-catenin pathways.

Neutrophils secrete net-like structures made of DNA and anti-microbial peptides, which can trap and kill extracellular pathogens. Here, the authors show that such nets are also produced by so-called Sentinel cells in the multicellular slug stage of the social amoeba Dictyostelium discoideum.

A position determination method for low-coherence interference fringe in optical fiber sensing is proposed. This method can be used to measure displacement or length. An amplitude ratio curve is created from spatially modulated interference fringes. There is a one-to-one correspondence relationship between the peaks of the amplitude ratio curve and the extremes of the low-coherence interference fringe. The peak positions of the amplitude ratio curve only rely on the Fabry-Pérot (F-P) cavity length. In addition, the peaks of the amplitude ratio curve are very sharp, which can be easily identified. In the calculation of the amplitude ratio curve, the low-coherence interference fringe is spatially modulated by Pockels effects. The impacts of the modulated voltage on the sensing precision are analytically and numerically discussed. Compared with the envelop detection method, the position determination precision of low-coherence interference fringe is enhanced about 30 times by creating an amplitude ratio curve. This method can be widely employed in the sensing of all kinds of parameters that can be converted to displacement or length.

Propofol is an intravenous sedative hypnotic agent of which the growth-inhibitory effect has been reported on various cancers. However, the roles of propofol in endometrial cancer (EC) remain unclear. This study aimed to explore the effects of propofol on EC in vitro and in vivo. Different concentrations of propofol were used to treat Ishikawa cells. Colony number, cell viability, cell cycle, apoptosis, migration, and invasion were analyzed by colony formation, MTT, flow cytometry, and Transwell assays. In addition, the pcDNA3.1-Sox4 and Sox4 siRNA plasmids were transfected into Ishikawa cells to explore the relationship between propofol and Sox4 in EC cell proliferation. Tumor weight in vivo was measured by xenograft tumor model assay. Protein levels of cell cycle-related factors, apoptosis-related factors, matrix metalloproteinases 9 (MMP9), matrix metalloproteinases 2 (MMP2) and Wnt/β-catenin pathway were examined by western blot. Results showed that propofol significantly decreased colony numbers, inhibited cell viability, migration, and invasion but promoted apoptosis in a dose-dependent manner in Ishikawa cells. Moreover, propofol reduced the expression of Sox4 in a dose-dependent manner. Additionally, propofol significantly suppressed the proportions of Ki67+ cells, but Sox4 overexpression reversed the results. Furthermore, in vivo assay results showed that propofol inhibited tumor growth; however, the inhibitory effect was abolished by Sox4 overexpression. Moreover, propofol inhibited Sox4 expression via inactivation of Wnt/β-catenin signal pathway. Our study demonstrated that propofol inhibited cell proliferation, migration, and invasion but promoted apoptosis by regulation of Sox4 in EC cells. These findings might indicate a novel treatment strategy for EC.

The development of alternative casting processes is essential for the high demand of light weight magnesium components to be used in the automotive industry, which often contain different section thicknesses. Squeeze casting with its inherent advantages has been approved for the capability of minimizing the gas porosity in magnesium alloys. For advanced engineering design of light magnesium automotive applications, it is critical to understand the effect of section thickness on mechanical properties of squeeze cast magnesium alloys. In this study, magnesium alloy AM60 with different section thicknesses of 6, 10 and 20 mm squeeze cast under an applied pressure of 30 MPa was investigated. The prepared squeeze cast AM60 specimens were tensile tested at room termperature. The results indicate that the mechanical properties including yield strength (YS), ultimate tensile strength (UTS) and elongation (A) decrease with an increase in section thickness of squeeze cast AM60. The microstructure analysis shows that the improvement in the tensile behavior of squeeze cast AM60 is primarily attributed to the low-gas porosity level and fine grain strucuture which result from the variation of cooling rate of different section thickness. The numerical simulation (Magmasoft? was employed to determine the solidification rates of each step, and the simulated results show that the solidification rate of the alloy decreases with an increase in the section thickness. The computed solidification rates support the experimental observation on grain structural development.

A chemical sensor for ozone based on an array of microfabricated tuning forks is described. The tuning forks are highly sensitive and stable, with low power consumption and cost. The selective detection is based on the specific reaction of the polymer with ozone. With a mass detection limit of ~2 pg/mm2 and response time of 1 second, the sensor coated with a polymer sensing material can detect ppb-level ozone in air. The sensor is integrated into a miniaturized wearable device containing a detection circuit, filtration, battery and wireless communication chip, which is ideal for personal and microenvironmental chemical exposure monitoring.

A heme-containing transmembrane ferric reductase domain (FRD) is found in bacterial and eukaryotic protein families, including ferric reductases (FRE), and NADPH oxidases (NOX). The aim of this study was to understand the phylogeny of the FRD superfamily. Bacteria contain FRD proteins consisting only of the ferric reductase domain, such as YedZ and short bFRE proteins. Full length FRE and NOX enzymes are mostly found in eukaryotic cells and all possess a dehydrogenase domain, allowing them to catalyze electron transfer from cytosolic NADPH to extracellular metal ions (FRE) or oxygen (NOX). Metazoa possess YedZ-related STEAP proteins, possibly derived from bacteria through horizontal gene transfer. Phylogenetic analyses suggests that FRE enzymes appeared early in evolution, followed by a transition towards EF-hand containing NOX enzymes (NOX5- and DUOX-like). An ancestral gene of the NOX(1-4) family probably lost the EF-hands and new regulatory mechanisms of increasing complexity evolved in this clade. Two signature motifs were identified: NOX enzymes are distinguished from FRE enzymes through a four amino acid motif spanning from transmembrane domain 3 (TM3) to TM4, and YedZ/STEAP proteins are identified by the replacement of the first canonical heme-spanning histidine by a highly conserved arginine. The FRD superfamily most likely originated in bacteria.

BACKGROUND: The association between polymorphisms on 15q25.1 and lung cancer has been widely evaluated; however, the studies have yielded contradictory results. We sought to investigate this inconsistency by performing a comprehensive meta-analysis on two polymorphisms (CHRNA3 gene: rs1051730 and AGPHD1 gene: rs8034191) on 15q25.1. METHODS: Data were extracted from 15 and 14 studies on polymorphisms rs1051730 and rs8034191 involving 12301/14000 and 14075/12873 lung cancer cases/controls, respectively. The random-effects model was applied, addressing heterogeneity and publication bias. RESULTS: The two polymorphisms followed Hardy-Weinberg equilibrium for all studies (P>0.05). For rs1051730-G/A, carriers of A allele had a 36% increased risk for lung cancer (95% confidence interval [CI]: 1.27-1.46; P