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IntroductionTo validate the effect of ethylene oxide sterilization of disposable electronic analgesia infusion pumps and determine the residual amount.MethodsAccording to ISO 11135：2014 Sterilization of Healthcare Products⁃Ethylene Oxide⁃Requirements for the Development， Validation and Routine Control of Sterilization Process for Medical Devices， qualification of physical cycle performance and microbial cycle performance were conducted on disposable electronic analgesia infusion pumps， and sterilization effect was then validated by using sterility test. According to ISO 10993⁃7：2008 Biological Evaluation of Medical Devices—Part 7： ethylene oxide sterilization residuals， the residual amount of ethylene oxide were further measured.ResultsThe sterilization effect fulfilled the requirements under the physical conditions of 38.0‒45.4 ℃ and 46%‒81% humidity. When the temperature was lower than 35 ℃ and the humidity was higher than 71%， the bacterial tablets remained fully activated after the sterilization with ethylene oxide. The shortest survival time without biological indicator after exposure to ethylene oxide was 8h. Furthermore， the residual amount of ethylene oxide after the sterilization was lower than the minimum detection limit.ConclusionAll tested products are sterilized. Sterilization equipment and sterilization process fulfilled the requirements of ISO 11135：2014. Additionally， the sterilization residual amount conforms to the limit values of ISO 10993⁃7：2008.

In the present study, the effects of 60Co-γ irradiation on the diversity of fungal microflora in blueberries during cold storage have been explored in detail. The effects of irradiation on the fungal microflora in blueberries could be observed at the lowest effective dose of 1.0 kGy. The genome library of fungal microflora in blueberries was evaluated by tusing Shannon, Simpson, Chao1, and ACE indices, presenting good coverage of blueberry samples. Principal coordinate analysis clarified the differences in the evolution and clustering of fungi in blueberries under different irradiation levels. The composition and diversity of fungal microflora in blueberries treated with different doses of radiation exhibited significant differences under cold storage of 4°C. However, most of the fungi belonged to the Ascomycota at the phylum. The fungal microflora was relatively stable at the initial stage of cold storage, however, the diversity of fungal species in irradiated blueberries varied greatly in the middle stage around the 15th day. This comprehensive study provides deeper insight about the effects of irradiation on the evolution of fungal microflora in blueberries during cold storage. The findings of this study lay a good foundation for post-harvest storage and preservation of blueberries. [ABSTRACT FROM AUTHOR]