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The microscopic mechanism of ferroelectric switching is the motion of domain walls, which is actually accomplished by the movement of tiny steps on the domain walls. Using first-principles calculations, the detailed polarization structures and the motion barriers of neutral and charged steps on 180° domain walls of prototypical ferroelectrics PbTiO3 are elaborately revealed in this study. While the Bloch components get weakened near all neutral steps, they become weakened/strengthened near the head-to-head/tail-to-tail charged steps. The neutral step possesses a lower formation energy but a higher migration barrier, indicating that the charged step could move faster. Based on these results, the possible motion picture of steps on one 180° domain wall of tetragonal ferroelectrics is proposed, which provides a better understanding of the mechanism of domain wall motion and may shed light on the future development of domain wall–based functional devices. [ABSTRACT FROM AUTHOR]

Neurodegenerative diseases (NDs) constitute a group of disorders characterized by the progressive deterioration of nervous system functionality. Currently, the precise etiological factors responsible for NDs remain incompletely elucidated, although it is probable that a combination of aging, genetic predisposition, and environmental stressors participate in this process. Accumulating evidence indicates that viral infections, especially neurotropic viruses, can contribute to the onset and progression of NDs. In this review, emerging evidence supporting the association between viral infection and NDs is summarized, and how the autophagy pathway mediated by viral infection can cause pathological aggregation of cellular proteins associated with various NDs is discussed. Furthermore, autophagy‐related genes (ARGs) involved in Herpes simplex virus (HSV‐1) infection and NDs are analyzed, and whether these genes could link HSV‐1 infection to NDs is discussed. Elucidating the mechanisms underlying NDs is critical for developing targeted therapeutic approaches that prevent the onset and slow the progression of NDs. [ABSTRACT FROM AUTHOR]

In this work, efficient microfluidic flow rate tuning based on the asymmetric flow of nematic liquid crystal 5CB under a horizontal temperature gradient is studied. Rectangular microchannels with the width of 100 μm are fabricated through soft lithography and treated with homeotropic surface anchoring conditions. Polarized optical microscopy is applied to explore the unique optical anisotropic characteristics of the nematic liquid crystal. The asymmetric velocity profiles in the microchannel are obtained by particle tracking velocimetry. The effects of temperature, flow rate, and aspect ratio on the velocity profile and split ratio of the asymmetric flow are quantitatively studied for the first time, while the mechanism of the flow asymmetry of the nematic liquid crystal is discussed. The results show that the asymmetric flow of the nematic liquid crystal occurs after the horizontal temperature gradient is applied, with the velocity in the heated region markedly higher than its counterpart. The split ratio of the asymmetric flow increases with the increase in the temperature gradient and the decrease in the flow rate. The aspect ratio influences the asymmetric flow through approaches of average velocity and surface anchoring strength, while the former is more distinct. The impacts of temperature gradient, flow rate, and aspect ratio on the flow asymmetry of nematic liquid crystals are caused by the coupling between physical properties, velocity field, and director field. Microchannels based on the asymmetric flow characteristics of nematic liquid crystals can act as a novel kind of temperature-controlled microvalve to achieve efficient microfluidic flow tuning. [ABSTRACT FROM AUTHOR]

Background: Familial non-medullary thyroid carcinoma (FNMTC) is a genetically predisposed disease with unclear genetic mechanisms. This makes research on susceptibility genes important for the diagnosis and treatment options. Methods: This study included a five-member family affected by papillary thyroid carcinoma. The candidate genes were identified through whole-exome sequencing and Sanger sequencing in family members, other FNMTC patients, and sporadic non-medullary thyroid carcinoma patients. The pathogenicity of the mutation was predicted using in silico tools. Cell phenotype experiments in vitro and models of lung distant metastasis in vivo were conducted to confirm the characteristics of the mutation. Transcriptome sequencing and mechanistic validation were employed to compare the disparities between PAK4 wild-type (WT) and PAK4 mutant (MUT) cell lines. Results: This mutation alters the protein structure, potentially increasing instability by affecting hydrophobicity, intra-molecular hydrogen bonding, and phosphorylation sites. It specifically promotes phosphorylated PAK4 nuclear translocation and expression in thyroid tissue and cell lines. Compared with the WT cells line, PAK4 I417T demonstrates enhanced proliferation, invasiveness, accelerated cell division, and inhibition of cell apoptosis in vitro. In addition, it exhibits a significant propensity for metastasis in vivo. It activates tumor necrosis factor signaling through increased phosphorylation of PAK4, JNK, NFκB, and c-Jun, unlike the WT that activates it via the PAK4-NFκ-MMP9 axis. In addition, PAK4 MUT protein interacts with matrix metalloproteinase (MMP)3 and regulates MMP3 promoter activity, which is not observed in the WT. Conclusions: Our study identified PAK4: c.T1250C: p.I417T as a potential susceptibility gene for FNMTC. The study concludes that the mutant form of PAK4 exhibits oncogenic function, suggesting its potential as a novel diagnostic molecular marker for FNMTC. [ABSTRACT FROM AUTHOR]

Chiral materials with circularly polarized luminescence (CPL) have garnered significant attention owing to their distinctive luminescent properties and wide array of applications. CPL enables the selective emission of left and right circularly polarized light. The fluorescence quantum yield and dissymmetry factor play pivotal roles in the generation of CPL. Helical polymers exhibit immense promise as CPL materials due to their inherent chirality, structural versatility, modifiability, and capacity to incorporate diverse chromophores. This Review provides a brief review of the synthesis of CPL materials based on helical polymers. The CPL can be realized by aggregation‐induced CPL of non‐emissive helical polymers, and helices bearing chromophores on the pendants and on the chain end. Furthermore, future challenges and potential applications of CPL materials are summarized and discussed. [ABSTRACT FROM AUTHOR]

Lithium‐metal batteries (LMBs) are regarded as a promising option for high‐energy‐density storage systems. However, lithium dendrite growth in LMBs can damage electrolytes and cause short circuits, which severely limits the practical application of LMBs. It is highly desired to develop an advanced polymer electrolyte with self‐healing capability and the ability to inhibit the growth of lithium dendrites for enhancing safety and prolonging the cycle life of the LMBs. Herein, sp3 boron‐based single‐ion conductor polymer electrolytes (B‐SIPEs) with excellent self‐healing capability and interfacial stability are constructed. The rapid bond exchange reactions of dynamic borate anions enable B‐SIPE to fast self‐heal in mild conditions, with a high self‐healing efficiency of 87% within 30 min at an ambient temperature. In particular, B‐SIPE displays a high Li+ transference number (0.88) and excellent lithium dendrite inhibition ability. The Li|B‐SIPE|Li cells exhibit a stable overpotential over 2000 h without short‐circuit. Additionally, the LiFePO4||Li cells with B‐SIPE exhibit superior long‐term cycling performance, with 93.5% capacity retention for 500 cycles. Significantly, B‐SIPE is capable of self‐healing the fracture in LiFePO4||Li cells and recovering the battery performance. These results demonstrate that the advanced self‐healing single‐ion conductor polymer electrolytes effectively promote the reliability and safety of LMBs. [ABSTRACT FROM AUTHOR]

Spatial scales are important for examining health of exploited fishery stocks and guiding management actions. However, information about the optimal spatial scale is still unclear for assessment of transit fisheries, such as elver fisheries of the American eel Anguilla rostrata. We applied a generalized depletion model to assess catch and effort data from three nearby rivers (within 50 km) to test the hypothesis that modeling on pooled and separate data from nearby rivers would give similar estimates of abundance and exploitation rate. Overall, pooling data from rivers within 50 km did not result in large differences (<20% in relative difference) in estimates of abundance and exploitation rate with close mean abundance estimates and similar temporal trends in abundance, exploitation rate, and relative escapement. Pooling nearby river systems can greatly reduce modeling effort, at the cost of ignoring fine‐scale variability in elver recruitment and having coarser spatial scale for the management. When only an index of annual recruitment and exploitation rate are of interest, pooling data may be practical from different locations up to 50 km. [ABSTRACT FROM AUTHOR]

Carbonic anhydrase 8 (CA8) is a member of the α‐carbonic anhydrase family but does not catalyze the reversible hydration of carbon dioxide. In the present study, we examined the effects of CA8 on two human colon cancer cell lines, SW480 and SW620, by suppressing CA8 expression through shRNA knockdown. Our results showed that knockdown of CA8 decreased cell growth and cell mobility in SW620 cells, but not in SW480 cells. In addition, downregulated CA8 resulted in a significant decrease of glucose uptake in both SW480 and SW620 cells. Interestingly, stable downregulation of CA8 decreased phosphofructokinase‐1 expression but increased glucose transporter 3 (GLUT3) levels in SW620 cells. However, transient downregulation of CA8 fails to up‐regulate GLUT3 expression, indicating that the increased GLUT3 observed in SW620‐shCA8 cells is a compensatory effect. In addition, the interaction between CA8 and GLUT3 was evidenced by pull‐down and IP assays. On the other hand, we showed that metformin, a first‐line drug for type II diabetes patients, significantly inhibited cell migration of SW620 cells, depending on the expressions of CA8 and focal adhesion kinase. Taken together, our data demonstrate that when compared to primary colon cancer SW480 cells, metastatic colon cancer SW620 cells respond differently to downregulated CA8, indicating that CA8 in more aggressive cancer cells may play a more important role in controlling cell survival and metformin response. CA8 may affect glucose metabolism‐ and cell invasion‐related molecules in colon cancer, suggesting that CA8 may be a potential target in future cancer therapy. Significance statement: This study demonstrates that when compared to primary colon cancer SW480 cells, metastatic colon cancer SW620 cells respond differently to downregulated CA8, indicating that CA8 in more aggressive cancer cells may play a more important role in controlling cell survival and metformin response. [ABSTRACT FROM AUTHOR]

The impact on child development of paternal involvement in childrearing has received growing attention recently. Based on data from the 2010, 2014 and 2018 waves of the China Family Panel Studies, this study applies the propensity score matching (PSM) method to investigate the causal effects of being primarily raised by one's father on children's cognitive skills and psychological well-being in different family types. In migrant families, primarily fathered children are not disadvantaged compared to those primarily mothered, and boys even have better word test scores. For children living with both parents, our results indicate no significant differences in most respects between the cognitive skills and mental health of children primarily cared for by fathers and by mothers, except that children perform slightly worse in word-test scores when their fathers are their primary caregivers. However, coresidence with grandparents substantially mitigates the negative effect of primary paternal involvement in childcare on children's language development. Our findings suggest that the role of father involvement in child development should be understood in more nuanced family contexts. [ABSTRACT FROM AUTHOR]

Traditional artificial skin gels often only focuses on a single perspective and overlooks the necessity of multidimensional synergistic design for sensitivity enhancement. To address these problems, the innovative introduction of dual solvents on the surface of polyacrylamide/sodium alginate gel induces to the transformation of supramolecular interactions including ion coordination, crystalline alcohol, and phase separation, synergistically achieving adjustable wrinkle wavelengths (304.2 ± 19.9 to 2393.5 ± 95.9 µm) and modulated chemical compositions with tunable moduli (87.5 ± 3.3 to 157.6 ± 3.7 kPa). This flexible strategy allows the construction of long‐range ordered wrinkled microstructures of three‐dimensional surfaces and complex morphologies. Meanwhile, the bridge effect induced by crystalline alcohols directs the insertion of ethanol molecules into the polymer chains, effectively reducing intramolecular friction. Benefiting from the small wavelength and low modulus dominated by crystalline alcohol, the wrinkled gel exhibits extremely high sensitivity of 164 kPa−1 (<0.5 kPa) and wide detection range (44.3 kPa). The wrinkled gel remains stable high sensitive to detect micro‐ and large stress, tactile perception, and human motion behaviors. This work proposes a new strategy for constructing highly sensitive bioinspired skin from multiple dimensions, showing broad application prospects in the fields of bioengineering and behavioral cognition. [ABSTRACT FROM AUTHOR]

Triple‐negative breast cancer (TNBC) is the most aggressive subtype of breast cancer (BC) compared to other BC subtypes in clinical settings. Currently, there are no effective therapeutic strategies for TNBC treatment. Therefore, there is an urgent need to identify suitable biomarkers or therapeutic targets for TNBC patients. Thrombomodulin (TM) plays a role in cancer progression and metastasis in many different cancers. However, the role of TM in TNBC is not yet fully understood. First, silenced‐TM in MDA‐MB‐231 cells caused an increase in proliferative and metastatic activity. In contrast, overexpression of TM in Hs578T cells caused a reduction in proliferation, invasion, and migration rate. Using RNA‐seq analysis, we found that Integrin beta 3 (ITGB3) expression may be a downstream target of TM. Furthermore, we found an increase in ITGB3 levels in TM‐KD cells by QPCR and western blot analysis but a decrease in ITGB3 levels in TM‐overexpressing cells. We found phospho‐smad2/3 levels were increased in TM‐KD cells but decreased in TM‐overexpressing cells. This implies that TM negatively regulates ITGB3 levels through the activation of the smad2/3 pathway. Silencing ITGB3 in TM‐KD cells caused a decrease in proliferation and migration. Finally, we found that higher ITGB3 levels were correlated with poor overall survival and relapse‐free survival in patients with TNBC. Our results indicated a novel regulatory relationship between TM and ITGB3 in TNBC. [ABSTRACT FROM AUTHOR]

Ovarian cancer is the most aggressive and lethal of all gynecologic malignancies. Although the overexpression (OE) of ubiquitin‐specific peptidase 21 (USP21) has been observed in multiple cancers, its expression profile and biological function in ovarian cancer remain unknown. The expression levels of USP21 in ovarian cancer cells and tissues as well as adjacent normal tissues were assessed by qRT‐PCR or Western blot assay. The biological function of USP21 in ovarian cancer cells was assessed by cell growth assay in vitro and a tumor growth model in vivo. Our study revealed that USP21 was markedly elevated in ovarian carcinoma tissues compared with adjacent normal tissues. Downregulation of USP21 attenuated the expression levels of MEK2 and p‐ERK1/2. Depletion of USP21 resulted in suppressed cell growth of ovarian cancers in vitro and inhibited tumor growth in vivo. Conversely, OE of USP21 promoted the cell proliferation of ovarian cancers and conferred resistance to BAY 11‐7082. These findings provide evidences supporting the notion of USP21 as a promising therapeutic target for the treatment of ovarian cancer. [ABSTRACT FROM AUTHOR]

Manganese slag (MS) is a kind of chemical waste, which may pollute the environment if conventional handling methods (stacking and landfill) are applied. Ultra-high-performance concrete (UHPC)—with considerably high compactness and strength—can be used not only as a special concrete material, but also to solidify the toxic substances in solid waste. This study proposes the addition of MS to UHPC, where the mass ratio of MS varies from 0% to 40% in the total mass of MS and silica fume. The effects of MS on the fluidity, plastic viscosity, and yield shear stress are investigated, and the flexural strength, compressive strength, and dry shrinkage rate of UHPC with MS are measured. X-ray diffraction (XRD) spectrum and energy spectrum analysis (EDS) diagrams are obtained to analyze the performance mechanism of the UHPC. A rheological study confirms that the slump flow increases with the increasing rate of 0–14.3%, while the yield shear stress and plastic viscosity decrease with the rates of 0–29.6% and 0–22.2%, respectively. The initial setting time increases with the mass ratio of MS by 0–14.3%, and MS has a positive effect on the flexural and compressive strengths of UHPC. In the early curing stage (less than 14 days), the increasing rate in the specimens increases with the curing age; meanwhile, when the curing age reaches 14 days or higher, the increasing rate decreases with increasing curing age. The compactness of UHPC is increased by adding MS. Furthermore, MS can increase the elements of Al and decrease crystals of Ca(OH)2 and calcium silicate hydrate in UHPC. [ABSTRACT FROM AUTHOR]

Hydrodynamic cavitation, which occurs when the local pressure is below the saturated vapor pressure in hydrodynamic flow, is ubiquitous in fluid dynamics and implicated in a myriad of industrial and biomedical applications. Although extensively studied in isotropic liquids, corresponding investigations in anisotropic liquids are largely lacking. In this paper, the hydrodynamic cavitation in the bluff body bypass flow of nematic liquid crystal 5CB in the microchannel is experimentally investigated. By 5CB, we mean the thermotropic liquid crystal 4′-pentyl-4-biphenylcarbonitrile. When the Reynolds number is in the range of 3 × 10−4 < Re < 1.2 × 10−3, a special flow phenomenon behind the bluff body is observed, namely, the disclination loop. The critical Reynolds number of cavitation inception varies with the shape of the bluff body, while the lowest value corresponds to the triangular bluff body. The hydrodynamic cavitation occurs in the Stokes flow regime with the Reynolds number significantly lower than 0.1 for all bluff bodies. There is a close relation between the oscillation behavior of cavitation domains and the structure of the bluff body. In addition, the pressure difference between the inlet and outlet of the microchannel shows linear relation with the Reynolds number rather than the quadratic relation for isotropic fluids, which proves the presence of shear thinning in the flow of nematic liquid crystals. The study in this paper on the hydrodynamic cavitation of nematic liquid crystal can broaden the research on providing new approaches for the enhancement of fluid mixing and heat transfer in microfluidic chips. [ABSTRACT FROM AUTHOR]

Parkinson's disease (PD), a neurodegenerative disorder with an unknown etiology, is primarily characterized by the degeneration of dopamine (DA) neurons. The prevalence of PD has experienced a significant surge in recent years. The unidentified etiology poses limitations to the development of effective therapeutic interventions for this condition. Helicobacter pylori (H. pylori) infection has affected approximately half of the global population. Mounting evidences suggest that H. pylori infection plays an important role in PD through various mechanisms. The autotoxin produced by H. pylori induces pro-inflammatory cytokines release, thereby facilitating the occurrence of central inflammation that leads to neuronal damage. Simultaneously, H. pylori disrupts the equilibrium of gastrointestinal microbiota with an overgrowth of bacteria in the small intestinal known as small intestinal bacterial overgrowth (SIBO). This dysbiosis of the gut flora influences the central nervous system (CNS) through microbiome-gut-brain axis. Moreover, SIBO hampers levodopa absorption and affects its therapeutic efficacy in the treatment of PD. Also, H. pylori promotes the production of defensins to regulate the permeability of the blood-brain barrier, facilitating the entry of harmful factors into the CNS. In addition, H. pylori has been found to induce gastroparesis, resulting in a prolonged transit time for levodopa to reach the small intestine. H. pylori may exploit levodopa to facilitate its own growth and proliferation, or it can inflict damage to the gastrointestinal mucosa, leading to gastrointestinal ulcers and impeding levodopa absorption. Here, this review focused on the role of H. pylori infection in PD from etiology, pathogenesis to levodopa bioavailability. [ABSTRACT FROM AUTHOR]

Although goal-directed fluid therapy (GDFT) has been proven to be effective in reducing the incidence of postoperative complications, the underlying mechanisms remain unknown. The aim of this study was to examine the mediating role of intraoperative hemodynamic lability in the association between GDFT and the incidence of postoperative complications. We further tested the role of this mediation effect using mean arterial pressure, a hemodynamic indicator. This secondary analysis used the dataset of a completed nonrandomized controlled study to investigate the effect of GDFT on the incidence of postoperative complications in patients undergoing posterior spine arthrodesis. We used a simple mediation model to test whether there was a mediation effect of average real variability between the association of GDFT and postoperative complications. We conducted mediation analysis using the mediation package in R (version 3.1.2), based on 5,000 bootstrapped samples, adjusting for covariates. Among the 300 patients in the study, 40% (120/300) developed postoperative complications within 30 days. GDFT was associated with fewer 30-day postoperative complications after adjustment for confounders (odds ratio: 0.460, 95% CI: 0.278, 0.761; P = 0.003). The total effect of GDFT on postoperative complications was –0.18 (95% CI : –0.28, –0.07; P < 0.01). The average causal mediation effect was –0.08 (95% CI : –0.15, –0.04; P < 0.01). The average direct effect was –0.09 (95% CI : –0.20, 0.03; P = 0.17). The proportion mediated was 49.9% (95% CI : 18.3%, 140.0%). The intraoperative blood pressure lability mediates the relationship between GDFT and the incidence of postoperative complications. Future research is needed to clarify whether actively reducing intraoperative blood pressure lability can prevent postoperative complications. [ABSTRACT FROM AUTHOR]

Objective: Polycystic ovarian syndrome (PCOS) is a prevalent gynecologic disorder, often associated with abnormal follicular development. Cangfu Daotan decoction (CFD) is a traditional Chinese medicine formula that is effective in alleviating PCOS clinically, but the specific mechanism remains unclear. Forkhead box K1 (FOXK1) is associated with cellular function. This study aimed to explore the effects of CFD and FOXK1 on PCOS.Methods: High-fat diet and letrozole were combined to establish PCOS rat models. Next, primary GCs were extracted from those PCOS rats. Then, GC cells were transfected with si-FOXK1 or oe-FOXK1. CFD-contain serum was prepared, and experiments were conducted to investigate the regulation of FOXK1 by CFD.Results: FOXK1 was highly expressed in GCs of PCOS rats. Further investigation revealed that FOXK1 overexpression resulted in inhibition of proliferation and DNA synthesis, along with promotion of apoptosis and autophagy in GCs. Additionally, it was found that FOXK1 promoted the expressions of the AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway-related proteins. Interestingly, treatment with CFD reversed all the effects of FOXK1 overexpression in GCs. Conclusion: This study demonstrated that CFD exerted a protective role in PCOS by inhibiting FOXK1, which provided a research basis for the application of CFD in PCOS, and suggested that FOXK1 is a novel therapeutic target in PCOS treatment. [ABSTRACT FROM AUTHOR]

Cyclic polymers have very unique structure and properties, and thus have drawn intense research attention. However, controlled synthesis of cyclic polymers with predictable molar mass and narrow distribution is still a challenging task. In this study, we developed a novel cyclic catalyst that initiates the ring-expansion polymerisation of isocyanides, producing a series of cyclic helical polymers with predictable molecular weight and low dispersity. Interestingly, the ring-expansion polymerization of the isocyanide macromonomers gives well-defined cyclic bottlebrush polymers. The cyclic topology was demonstrated using transmission electron microscopy. [ABSTRACT FROM AUTHOR]

Two-dimensional (2D) transitional metal dichalcogenides (TMDs) have garnered remarkable attention in electronics, optoelectronics, and hydrogen precipitation catalysis due to their exceptional physicochemical properties. Their utilisation in optoelectronic devices is especially notable for overcoming graphene's zero-band gap limitation. Moreover, TMDs offer advantages such as direct band gap transitions, high carrier mobility, and efficient switching ratios. Achieving precise adjustments to the electronic properties and band gap of 2D semiconductor materials is crucial for enhancing their capabilities. Researchers have explored the creation of 2D alloy phases through heteroatom doping, a strategy employed to fine-tune the band structure of these materials. Current research on 2D alloy materials encompasses diverse aspects like synthesis methods, catalytic reactions, energy band modulation, high-voltage phase transitions, and potential applications in electronics and optoelectronics. This paper comprehensively analyses 2D TMD alloy materials, covering their growth, preparation, optoelectronic properties, and various applications including hydrogen evolution reaction catalysis, field-effect transistors, lithium-sulphur battery catalysts, and lasers. The growth process and characterisation techniques are introduced, followed by a summary of the optoelectronic properties of these materials. [ABSTRACT FROM AUTHOR]

Here, the multibranch linear–linear basis function (MB‐LL) is applied to the Poggio–Miller–Chang–Harrington–Wu–Tsai equation for calculating the scattering problem of multi‐scale dielectric targets. The MB‐LL basis function replaces the positive and negative triangles of the traditional linear–linear basis function (LL) with multiple branches, so it can be used at the junction of the meshes of different size. By using the MB‐LL basis function, we can easily divide the calculation region into several non‐overlapping regions, which is very suitable for the simulation of multiscale targets. At the same time, the MB‐LL basis function inherits the properties of the LL basis function, which is more accurate than the multibranch Rao–Wilton–Glisson (MB‐RWG) basis function. Numerical examples demonstrate the advantages of the proposed method. [ABSTRACT FROM AUTHOR]

Summary: Plant height is an important agronomic trait that affects crop yield. Elucidating the molecular mechanism underlying plant height regulation is also an important question in developmental biology.Here, we report that a BELL transcription factor, ZmBELL10, positively regulates plant height in maize (Zea mays). Loss of ZmBELL10 function resulted in shorter internodes, fewer nodes, and smaller kernels, while ZmBELL10 overexpression increased plant height and hundred‐kernel weight. Transcriptome analysis and chromatin immunoprecipitation followed by sequencing showed that ZmBELL10 recognizes specific sequences in the promoter of its target genes and activates cell division‐ and cell elongation‐related gene expression, thereby influencing node number and internode length in maize.ZmBELL10 interacted with several other ZmBELL proteins via a spatial structure in its POX domain to form protein complexes involving ZmBELL10. All interacting proteins recognized the same DNA sequences, and their interaction with ZmBELL10 increased target gene expression. We identified the key residues in the POX domain of ZmBELL10 responsible for its protein–protein interactions, but these residues did not affect its transactivation activity.Collectively, our findings shed light on the functions of ZmBELL10 protein complexes and provide potential targets for improving plant architecture and yield in maize. [ABSTRACT FROM AUTHOR]

In this study, we investigate the potential changes of El Niño characteristics, including intensity, frequency and CP/EP El Niño ratio, under progressive global warming based on the 140-year CMIP6 model simulation outputs with the 1pctCO2 experiment. Major air-sea feedback mechanisms attributing to the changes are also examined. The CMIP6 ensemble means project a slight enhancement of El Niño intensity by about 2% and a modest increase of El Niño frequency by about 4% from the first to the second 70-year periods. It is found that these small changes result from the opposite response to global warming between CP and EP El Niño, i.e., the intensity of EP El Niño is projected to weaken by nearly 4.6% while the intensity of CP El Niño is projected to increase by about 4.5%. Since CP El Niño occurs more frequently than EP El Niño in CMIP6 simulations, this leads to a slight enhancement of the total El Niño intensity if these two types of El Niño were not separated. A similar situation occurs in projecting the future change of El Niño frequency, i.e., the frequency of EP El Niño is projected to decrease by about 1.4% while the frequency of CP El Niño is projected to increase by about 2%, thereby leading to a modest increase of the total El Niño frequency. By comparing the variance explained by key air-sea feedback mechanism between the two 70-year periods, we also note that the increased CP/EP ratio can be explained by the enhanced role played by the SF (seasonal footprinting) mechanism in a warmer atmosphere. Our study also points out that, as long as a climate model can correctly produce the intensity (variance) of major air-sea feedback mechanisms, the relationship between changes in El Niño characteristics and changes in feedback mechanisms can be physically robust. Key points: The potential changes of El Niño characteristics are investigated based on 140-year CMIP6 simulation outputs with the 1pctCO2 experiment. The ensemble means project a slight enhancement of El Niño intensity (2%) and a modest increase of El Niño frequency (4%) from the first to the second 70-year periods. CP El Niño becomes stronger and more frequent and CP/EP ratio increases due to the enhanced SF mechanism in a warmer atmosphere. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the role of long non-coding RNA (lncRNA) small nucleolar RNA host gene 4 (SNHG4) in diabetic retinopathy (DR). We found that SNHG4 was downregulated in DR. SNHG4 could directly interact with miR-200b, while overexpression of miR-200b did not affect the expression of SNHG4 in human retinal pigment epithelial cells ARPE-19. In contrast, overexpression of SNHG4 led to the upregulation of oxidation resistance 1 (Oxr1), a target of miR-200b. Cell apoptosis analysis showed that overexpression of miR-200b increased the apoptotic rate of ARPE-19 cells under high glucose treatment. Oxr1 and SNHG4 played opposite roles and reduced the effects of overexpression of miR-200b. In conclusion, SNHG4 may sponge miR-200b to inhibit cell apoptosis in DR by upregulating Oxr1. [ABSTRACT FROM AUTHOR]

Objectives: Aortic carboxypeptidase‐like protein (ACLP) is an extracellular protein involved in adipogenesis, epithelial‐mesenchymal transition, epithelial cell hyperplasia, and collagen fibrogenesis. This study mainly aimed to analyze the potential role of adipocyte enhancer binding protein 1 (AEBP1), the ACLP‐encoding gene, as a pathological target or prognostic marker for liver fibrosis regardless of etiology. Methods: Dysregulation pattern, clinical relevance, and biological significance of AEBP1 gene in liver fibrosis were analyzed using publicly available transcriptomic profiles, different liver fibrosis mouse models, biological databases, and AEBP1 gene silencing followed by RNA sequencing in human hepatic stellate cells (HSCs). Results: AEBP1 gene expression was upregulated and positively correlated with liver fibrogenesis independent of etiology, the protein of which was further verified in liver fibrosis mouse models induced by different pathogenic factors. A higher expression of liver AEBP1 gene had the potential to predict poor prognosis in liver fibrosis. Systematic bioinformatic analyses revealed that AEBP1 expression was HSCs‐specific and associated with extracellular matrix (ECM) remodeling and its downstream mechanical–chemical signaling transition. AEBP1 knockdown by specific small interfering RNAs (siRNAs) in HSCs inhibited ECM‐receptor interaction and immune‐related pathways as well as HSC proliferation or activation. Conclusion: A high expression of AEBP1 was specifically associated with liver fibrosis and was related to a poor prognosis and predicted the role of AEBP1 in HSCs, providing a new insight for understanding AEBP1 in liver fibrosis. [ABSTRACT FROM AUTHOR]

In this study, we contrast the distinct energy recharge-discharge cycle between propagating and eastward-decaying Madden–Julian Oscillation (MJO) events during the boreal winter season (November–April) from 1979 to 2018 using the moist static energy (MSE) budget and gross moist stability (GMS) plane analyses. A total of 41 MJO events are selected during the 40-year period, including 16 strong propagating (SP), 13 weak propagating (WP) and 12 eastward-decaying (ED) types of MJOs. The column-integrated MSE budget shows that, depending on the phases, vertical and horizontal advection terms take turns leading the role in discharging or recharging the MSE. In the phase when the net flux heating reaches the maximum, vertical advection plays the lead role in discharging the MSE. In the subsequent phases when the net flux heating gradually weakens, horizontal advection becomes the dominant factor in discharging the MSE. A similar situation occurs during the recharge phases to balance the net flux cooling. The SP MJO exhibits a stronger energy recharge-discharge cycle compared to the WP and ED MJOs, and the contrast significantly enlarges from the Indian Ocean to the Maritime Continent. The GMS plane analysis further reveals that four different types of convection attribute to the recharge-discharge process. During the wet phases, the top-heavy ascending motion and negative shallow convection jointly stabilize the atmosphere by exporting the MSE; while during the dry phases, the top-heavy descending motion and the positive shallow convection together destabilize the atmosphere through importing the MSE. Moreover, the recharge (discharge) phase leads the amplifying (decaying) phase by a quarter cycle is essential to maintain a robust energy recharge-discharge cycle to drive the eastward propagating MJOs. [ABSTRACT FROM AUTHOR]

Wound healing is a complex, dynamic process supported by a myriad of cellular events that must be tightly coordinated to efficiently repair damaged tissue. These wounds are a significant socioeconomic burden due to their high prevalence and recurrence, which is why the phenomenon of wounds has also been labeled as a "Silent Epidemic". Most of these wounds become "chronic", with around 15% of them remaining unresolved 1-year post incidence, which results in a prolonged yet avoidable burden to patients, families, and the health system. In this experimental study, we tried to purify the potent components in chick early amniotic fluid (ceAF) and applied these components to the wound healing mechanism. We first subjected ceAF to a series of purifications, including an HPLC purification system along with ion-exchange chromatography technology to purify other potential components. Upon narrowing down, we found two structural analogs: guanosine and deoxyinosine. We performed these components' cell scratch and trans-well migration assays to validate the accurate dosage. We also assessed these components via topical administration on the skin of murine model wounds. For this, we randomly divided C57BL/6 (all black, male, 5 weeks old) mice into groups. The wound model was established through excising the skin of mice and treated the wounds with different fractions of guanosine and deoxyinosine continuously for 8–10 day intervals. Once the healing was complete, the skin was excised to determine the inflammatory response and other biochemical parameters of the healed skin, including epidermal thickness, collagen density, macrophages, and neutrophil infiltration at the wounded site. Quantitative real-time PCR and immunoblot assays were performed to determine active gene expression and protein expression of proinflammatory molecules, growth factors, and cytokines. All these findings support our data indicating the promising healing properties of guanosine and deoxyinosine isolated from ceAF. [ABSTRACT FROM AUTHOR]

Regioselective B–H activation of o-carboranes is an effective way for constructing o-carborane derivatives, which have broad applications in medicine, catalysis and the wider chemical industry. However, the mechanistic basis for the observed selectivities remains unresolved. Herein, a series of density functional theory (DFT) calculations were employed to characterise the palladium N-heterocyclic carbene (Pd-NHC) catalysed regioselective B(3,6)-diarylation of o-carboranes. Computational results at the IDSCRF(ether)-LC-ωPBE/BS1 and IDSCRF(ether)-LC-ωPBE/BS2 levels showed that the reaction undergoes a Pd(0) → Pd(II) → Pd(0) oxidation/reduction cycle, with the regioselective B(3)–H activation being the rate-determining step (RDS) for the full reaction profile. The computed RDS free energy barrier of 24.3 kcal mol−1 agrees well with the 82% yield of B(3,6)-diphenyl-o-carborane in ether solution at 298 K after 24 hours of reaction. The Ag2CO3 additive was shown to play a crucial role in lowering the RDS free energy barrier and facilitating the reaction. Natural charge population (NPA) and molecular surface electrostatic potential (ESP) analyses successfully predicted the experimentally observed regioselectivities, with electronic effects being revealed to be the dominant contributors to product selectivity. Steric hindrance was also shown to impact the reaction rate, as revealed by experimental and computational characterisation studies of substituents and ligand effects. Furthermore, computational predictions aligned with the experimental findings that NHC ligands outperform the phosphine ones for this particular reaction. Overall, the observed trends reported in this work are expected to assist in the rational optimisation of the efficiency and regioselectivity of this and related reactions. [ABSTRACT FROM AUTHOR]

Although hydrogen has been recognized as a promising material for global energy transportation, its inadvertent leakage or generation into enclosed spaces may pose a risk of an explosion hazard in engineering facilities, including nuclear power plants. To manage the associated risks, it is crucial to identify the characteristics of hydrogen dispersion in air and its stratification behavior. In this study, we conducted an experimental investigation on the distribution of hydrogen in an enclosed vessel under steam-condensing conditions by using helium as a substitute for hydrogen. A series of steady-state and transient tests were carried out in a cylindrical test vessel, in which the interacting effect with steam condensation was simulated by employing a vertical condenser tube to promote the mixing of the steam-air-helium mixture. During transient tests, the impact of the jet momentum created by helium injection into the enclosure and subsequent buoyancy-driven redistribution of helium in the postinjection phase was observed. [ABSTRACT FROM AUTHOR]

Objective: SNRPA1, a subunit of spliceosome complex, has been implicated in diverse cancers, while its biological effect in LUAD remains elusive. Therefore, we sought to decipher the relationship between SNRPA1 expression and the prognosis of patients with LUAD and reveal the underlying molecular mechanism. Materials and methods: Based on the clinical data from TCGA databases, the multivariate Cox model was constructed to screen the prognostic value of SNRPA1. qRT‐PCR and immunohistochemical staining were used to examine SNRPA1 mRNA and protein expression in LUAD. The effect of SNRPA1 on LUAD cell proliferation, migration, and epithelial mesenchymal transformation were examined using colony formation assays, wound healing, and western blot assays, respectively. Finally, the influence of SNRPA1 on LUAD immune microenvironment were validated from the Tumor Immune Estimation Resource database. Results: SNRPA1 was significantly upregulated in both LUAD tissues and cell lines, and highly expressed SNRPA1 contributed to poor prognosis of LUAD patients. In vitro, SNRPA1 knockdown inhibited the proliferation and migration, as well as delayed the EMT differentiation of LUAD cells. Lastly, SNRPA1 was found to be positively associated with immune infiltration and some immune‐check‐point markers. Conclusions: Our findings indicate that SNRPA1 may be a new biomarker for prognostic prediction and a potential therapeutic target in the treatment of LUAD. [ABSTRACT FROM AUTHOR]

Background: Colorectal cancer (CRC) is highly prevalent and lethal globally, and its prognosis remains unsatisfactory. Drug resistance is regarded as the main cause of treatment failure leading to tumor recurrence and metastasis. The overexpression of fucosylated epitopes, which are usually modifications of glycoproteins, was reported to occur in various epithelial cancers. However, the effects of treatments that target these antigens in colorectal cancer remain unclear. Methods: This study investigated the expression of heavily fucosylated glycans (HFGs) in 30 clinical samples from patients with CRC and other normal human tissues. The complement-dependent cytotoxicity was explored in vitro through treatment with anti-HFG monoclonal antibody (mAb) alone or in combination with chemotherapeutic agents. In vivo inhibitory effects were also examined using a xenograft mouse model. Results: Immunohistochemistry staining and western blotting revealed that HFG expression was higher in human colorectal cancer tissues than in normal tissues. In DLD-1 and SW1116 cells, which overexpress fucosylated epitopes, anti-HFG mAb produced observable cytotoxic effects, especially when it was combined with chemotherapeutic agents. The xenograft model also demonstrated that anti-HFG mAb had potent and dose-dependent inhibitory effects on colorectal tumor growth. Conclusions: As a novel cancer antigen, HFGs are a promising treatment target, and the implementation of anti-HFG mAb treatment for CRC warrants further investigation. [ABSTRACT FROM AUTHOR]

Background and objectives: Tacrolimus (TAC) has been increasingly used in patients with non-transplant settings. Because of its large between-subject variability, several population pharmacokinetic (PPK) studies have been performed to facilitate individualized therapy. This review summarized published PPK models of TAC in non-transplant patients, aiming to clarify factors affecting PKs of TAC and identify the knowledge gap that may require further research. Methods: The PubMed, Embase databases, and Cochrane Library, as well as related references, were searched from the time of inception of the databases to February 2023, to identify TAC population pharmacokinetic studies modeled in non-transplant patients using a non-linear mixed-effects modeling approach. Results: Sixteen studies, all from Asian countries (China and Korea), were included in this study. Of these studies, eleven and four were carried out in pediatric and adult patients, respectively. One-compartment models were the commonly used structural models for TAC. The apparent clearance (CL/F) of TAC ranged from 2.05 to 30.9 L·h−1 (median of 14.9 L·h−1). Coadministered medication, genetic factors, and weight were the most common covariates affecting TAC-CL/F, and variability in the apparent volume of distribution (V/F) was largely explained by weight. Coadministration with Wuzhi capsules reduced CL/F by about 19 to 43%. For patients with CYP3A5*1*1 and *1*3 genotypes, the CL/F was 39–149% higher CL/F than patients with CYP3A5*1*1. Conclusion: The optimal TAC dosage should be adjusted based on the patient's co-administration, body weight, and genetic information (especially CYP3A5 genotype). Further studies are needed to assess the generalizability of the published models to other ethnic groups. Moreover, external validation should be frequently performed to improve the clinical practicality of the models. [ABSTRACT FROM AUTHOR]

Ischemic stroke is featured with high incidence, mortality, and disability. The aim of this study is to use Global Burden of Disease database to describe and compare the burden of ischemic stroke in mainland China and Taiwan province and to further predict the expected changes in the next 11 years using statistical modeling methods. Information on ischemic stroke incidence and mortality in China (mainland and Taiwan province) during 1990–2019 was obtained from the Global Burden of Disease database to analyze the effects of region, gender, and age on the incidence and mortality of ischemic stroke in China. The autoregressive integrated moving average model was used to predict the age-standardized incidence rate and age-standardized mortality rate of ischemic stroke in males and females in mainland China and Taiwan province in the next 11 years. The period from 1990 to 2019 witnessed an overall upward trend in the number of incidence and deaths in mainland China and Taiwan province. In 2019, there were nearly 2.87 million ischemic incidence cases with stroke in mainland China, with more female patients than male in the age group of over 60 years. Among the nearly 1.03 million deaths, the death toll of men under the age of 85 years was higher than that of women, while in Taiwan province, the number of incidence was 28 771, with more female patients of all ages than male. Among the 6788 deaths, the death toll of men under the age of 80 years was higher than that of women. In 2019, the age group with the highest number of patients in the two regions was 65–69 years, while the highest number of deaths was found in people aged 85 years and above. As our autoregressive integrated moving average model predicted, the age-standardized incidence rate value of ischemic stroke is expected to be 163.23/100 000 persons in mainland China by 2030, which would continue to increase, while the age-standardized mortality rate value of ischemic stroke is expected to be 16.41/100 000 persons in Taiwan province by 2030, which showed a decreasing trend. Disease burden of ischemic stroke is still increasing in mainland China and Taiwan province, and health resources should be deployed to implement effective prevention and control strategies, taking into account region, gender, and age. [ABSTRACT FROM AUTHOR]

Porcine reproductive and respiratory syndrome virus (PRRSV) poses a global threat to pig health and results in significant economic losses. Impaired innate and adaptive immune responses are evident during PRRSV infection. Cyclic GMP-AMP synthase (cGAS), a classical pattern recognition receptor recognizing mainly intracytoplasmic DNA, induces type I IFN responses through the cGAS-STING signaling pathway. It has also been demonstrated that cGAS-STING is involved in PRRSV infection. This study utilized the qRT-PCR, ELISA, and WB methods to examine the effects of Astragaloside IV (AS-IV) on the regulation of innate immune function and cGAS-STING signaling pathway in porcine alveolar macrophages. The results showed that AS-IV attenuated the decreased innate immune function caused by PRRSV infection, restored the inhibited cGAS-STING signaling pathway, and increased the expression of interferon, ultimately exerting antiviral effects. Moreover, these results suggest that AS-IV may be a promising candidate for a new anti-PRRSV antiviral, and its mechanism of action may provide insights for developing novel antiviral agents. [ABSTRACT FROM AUTHOR]

Objective Hospital-acquired pneumonia (HAP) is one of the most common diseases in the intensive care unit, where the development of disease is closely related with the host immune response. Monocytes play an important role in both innate and adaptive immune system. We aimed to investigate the changes of circulating monocyte subsets in subjects with HAP to explore its value in monitoring HAP. Methods In total, 60 HAP patients and 18 healthy individuals were enrolled in this study. Human monocyte subsets are classified into 3 groups: nonclassical (NC), intermediate (ITM), and classical (CL). Also, programmed death ligand 1 (PD-L1) expression on circulating monocyte subsets was measured by flow cytometry. Results Data showed that the ratio of NC, ITM, and CL among monocytes was comparable between HAP patients and healthy controls (P  > .05). There was a remarkable imbalance of NC and CL in newly emerged HAP compared to healthy controls (P  < .05), subsequently reaching normalization in recurrent HAP (P  > .05). Furthermore, although PD-L1 was seemly constitutively expressed by NC, ITM, and CL groups regardless of disease status, it was noted that PD-L1 was dominantly expressed in the CL group (P  < .05). Conclusion Given distinct PD-L1 expression, a shift of CL/NC in newly emerged HAP would constitute an inhibitory anti-pathogen immune response. Normalization of circulating monocyte subsets on recurrence of HAP might be the consequence of immune memory of bacterial infection. [ABSTRACT FROM AUTHOR]

Background: Coronavirus disease 2019 (COVID-19) poses a significant threat to the safety of residents in long-term care facilities, and the staff of long-term care facilities are essential in the care and prevention of major infectious diseases and therefore require good health literacy to ensure the health of residents. The main objective of this study was to examine the health literacy of staff in long-term care facilities and analyze the factors associated with their COVID-19 health literacy in Taiwan to provide a basis for the response mechanism to emerging infectious diseases. Methods: A cross-sectional survey with a structured questionnaire by a convenience sample method and to assess the COVID-19 health literacy of caregivers working in long-term care facilities in this study. The COVID-19 health literacy scale was a self-administered scale designed to combine the concept of "health literacy" with the 3 levels and 5 stages of preventive medicine. A total of 385 workers from 10 long-term care facilities were surveyed as the study sample, and the validated questionnaires were statistically analyzed using SPSS version 22.0 statistical software. A multivariate logistic regression model was used to establish the associated factors of the COVID-19 health literacy level. Results: Overall, the mean COVID-19 health literacy score was 88.7 ± 10.4 (range: 58–105). Using a quartile scale, 92 (23.9%) of the study participants had low health literacy (health literacy score < 82), 190 (49.3%) had average health literacy (health literacy score 82–98), and the remaining 103 (26.8%) had good health literacy (health literacy score 99–105). Statistical analysis revealed significant differences (p < 0.05) in the COVID-19 health literacy score by demographic variables (education, job category, number of daily service users, and training related to infectious disease prevention and control) of the study population. The logistic regression analysis of the COVID-19 health literacy level (> 82 vs. ≤82) showed a significant difference in the study sample by gender (male vs. female, OR = 2.46, 95% CI = 1.15–5.26), job category (nurse practitioner vs. caregiver, OR = 7.25, 95% CI = 2.46–21.44), monthly service hours (> 160 h vs. 40–79 h, OR = 0.044, 95% CI = 0.07–0.97), experience caring for confirmed COVID-19 patients (yes vs. no, OR = 0.13, 95% CI = 0.02–0.98), and training related to infectious disease prevention and control (yes vs. no, OR = 2.8, 95% CI = 1.52–5.15). Conclusions: This study recommends that facilities provide immediate updated COVID-19 information to staff, especially frontline caregivers, and specifically enhance COVID-19 infection control education training for all facility staff to eliminate health literacy disparities. [ABSTRACT FROM AUTHOR]

Multiple inositol polyphosphate phosphatase (MINPP1) is an enigmatic enzyme that is responsible for the metabolism of inositol hexakisphosphate (InsP6) and inositol 1,3,4,5,6 pentakisphosphate (Ins(1,3,4,5,6)P5 in mammalian cells, despite being restricted to the confines of the ER. The reason for this compartmentalization is unclear. In our previous studies in the insulin-secreting HIT cell line, we expressed MINPP1 in the cytosol to artificially reduce the concentration of these higher inositol phosphates. Undocumented at the time, we noted cytosolic MINPP1 expression reduced cell growth. We were struck by the similarities in substrate preference between a number of different enzymes that are able to metabolize both inositol phosphates and lipids, notably IPMK and PTEN. MINPP1 was first characterized as a phosphatase that could remove the 3-phosphate from inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4). This molecule shares strong structural homology with the major product of the growth-promoting Phosphatidyl 3-kinase (PI3K), phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) and PTEN can degrade both this lipid and Ins(1,3,4,5)P4. Because of this similar substrate preference, we postulated that the cytosolic version of MINPP1 (cyt-MINPP1) may not only attack inositol polyphosphates but also PtdIns(3,4,5)P3, a key signal in mitogenesis. Our experiments show that expression of cyt-MINPP1 in HIT cells lowers the concentration of PtdIns(3,4,5)P3. We conclude this reflects a direct effect of MINPP1 upon the lipid because cyt-MINPP1 actively dephosphorylates synthetic, di(C4:0)PtdIns(3,4,5)P3 in vitro. These data illustrate the importance of MINPP1′s confinement to the ER whereby important aspects of inositol phosphate metabolism and inositol lipid signaling can be separately regulated and give one important clarification for MINPP1′s ER seclusion. [ABSTRACT FROM AUTHOR]

Background: Dignity‐conserved nursing has been widely studied by scholars all over the world; however, there is no clear direction in which this field is trending. Aim: To conduct a bibliometric analysis that systematically characterises publications on dignity research in the nursing field from 2011 to 2020. Design: Bibliometric and visual analysis of retrieved articles. Methods: The Web of Science Core Collection database was used to retrieve all articles which addressed dignity in nursing from 2011 to 2020. The WoSCC's own analysis tool, CiteSpace and VOSviewer, were used to obtain visual analysis results. Reporting follows the STROBE checklist. Results: A total of 1429 papers on dignity care are included in this study. We found that the number of papers on this topic increased steadily, and the United States topped the list with 366 articles in total. The institute with the most publications was King's College London, and the most widely published journal was Nursing Ethics. We were able to identify four major research topics, namely dignity in: (a) palliative care, (b) dementia and the elderly, (c) health care and (d) nursing ethics. Terminally ill patient, home, value, rehabilitation and psychological distress were the five keywords with the highest burst strength. Conclusions: The interest in dignity care research has been steadily increasing from 2011 and is reflected in the number of published papers. The United States and Western Europe are leading in this field, both having a high number of cutting‐edge researchers and high‐level scientific research institutions. In the domain of dignity care, several stable and high‐yield core author groups have been formed. While the existing research mainly focuses on four hot spots, psychological distress, advanced cancer, maternity care and content analysis may be the research frontiers. [ABSTRACT FROM AUTHOR]

Construction activity sequence changes are among the most crucial considerations in establishing construction simulation models. However, conventional simulation models are designed with a fixed sequence of simulation activities, which is unable to update automatically. Existing machine learning methods require abundant time for manual labelling and are unsuitable for construction data with high‐dimensional and heterogeneous characteristics. The motivation for this work is to develop a labour‐saving activity sequence classification model for earthwork construction simulation which realizes flexibly modifying simulation activities according to different weather conditions. Three heterogeneous semi‐supervised classifiers with complementary characteristics were ensembled to reduce the workload of manual labelling and enhance the generalization ability of activity sequence classification based on weather data. Furthermore, Dempster–Shafer‐based evidence reasoning improved by a security filtering mechanism was adopted to enhance the accuracy of semi‐supervised classification. The proposed enhanced ensemble semi‐supervised activity sequence classification model was embedded in an earthwork construction simulation model, which was evaluated in a case study of rockfill dam construction. The proposed classifier outperformed four common semi‐supervised methods and two common supervised methods in terms of accuracy and generalization ability. Additionally, the proposed simulation method outperformed conventional simulation methods in terms of the construction schedule, construction intensity and consistency of the simulated activity sequence with the true values by 65.55%, 28.47% and 88.15%, respectively. [ABSTRACT FROM AUTHOR]

Treatment of epithelial ovarian cancer (EOC) is a challenge because it still leads to unsatisfactory clinical prognosis. This is due to the toxicity and poor targeting of chemotherapeutic agents, as well as metastasis of the tumor. In this study, we designed a targeted liposome with nanostructures to overcome these problems. In the liposomes, epirubicin and curcumin were encapsulated to achieve their synergistic antitumor efficacy, while Epi-1 was modified on the liposomal surface to target epithelial cell adhesion molecule (EpCAM). Epi-1, a macrocyclic peptide, exhibits active targeting for enhanced cellular uptake and potent cytotoxicity against tumor cells. The encapsulation of epirubicin and curcumin synergistically inhibited the formation of neovascularization and vasculogenic mimicry (VM) channels, thereby suppressing tumor metastasis on SKOV3 cells. The dual drug loaded Epi-1-liposomes also induced apoptosis and downregulated metastasis-related proteins for effective antitumor in vitro. In vivo studies showed that dual drug loaded Epi-1-liposomes prolonged circulation time in the blood and increased the selective accumulation of drug at the tumor site. H&E staining and immunohistochemistry with Ki-67 also showed that targeted liposomes elevated antitumor activity. Also, targeted liposomes downregulated angiogenesis-related proteins to inhibit angiogenesis and thus tumor metastasis. In conclusion, the production of dual drug loaded Epi-1-liposomes is an effective strategy for the treatment of EOC. [ABSTRACT FROM AUTHOR]

The copper containing glass, particularly with rich Cu+ ions, is a promising material for antibacterial and luminescent. The valence control of Cu+/Cu2+ in melt-quenching glass has been widely investigated, but sol-gel method was rarely extended. Herein, the Cu+ rich silicate glass was successfully prepared by introducing the P 2 O 5 as modifying agent in the sol-gel method fabricated phosphosilicate glasses, briefed as 10CaO-(90-x)SiO 2 -xP 2 O 5 (x = 0, 2.5, 5, 7.5, 10 mol%)- 5CuO. It is found that the high content of P 2 O 5 will generates more Cu+ in the gel forming stage and result in a Cu+ rich glass. While, the content of Cu+ drops with excessive P 2 O 5 additives in the prepared glass. The highest Cu+ content was obtained in glass with 7.5 mol% P 2 O 5. Because phosphate glass network is looser than silica-oxygen glass network, which lead to copper ion in the phosphate cluster as a charge compensator is easier to be oxidized compared with in the Si–O network. Therefore, our study paved a new way for designing Cu+ rich glass by sol-gel method. [ABSTRACT FROM AUTHOR]

A layered double hydroxide (LDH) calcined-framework adsorbent was investigated for the rapid removal of heavy metal cations from plating wastewater. Li–Al–CO3 LDH was synthesized on an aluminum lathe waste frame surface to prepare the sorbent. The calcination treatment modified the LDH surface properties, such as the hydrophilicity and the surface pH. The change in surface functional groups and the leaching of lithium ions affected the surface properties and the adsorption capacity of the heavy metal cations. A zeta potential analysis confirmed that the 400 °C calcination changed the LDH surface from positively charged (+10 mV) to negatively charged (−17 mV). This negatively charged surface contributed to the sorbent instantly bonding with heavy metal cations in large quantities, as occurs during contact with wastewater. The adsorption isotherms could be fitted using the Freundlich model. The pseudo-second-order model and the rate-controlled liquid-film diffusion model successfully simulated the adsorption kinetics, suggesting that the critical adsorption step was a heterogeneous surface reaction. This study also confirmed that the recovered nickel and/or copper species could be converted into supported metal nanoparticles with a high-temperature hydrogen reduction treatment, which could be reused as catalysts. [ABSTRACT FROM AUTHOR]

Background: Autophagy is a lysosome-mediated catabolic process that maintains cell homeostasis and survival. It occurs not only in normal cells such as cardiac muscle cells, neurons, and pancreatic acinar cells but also in various benign and malignant tumors. The abnormal level of intracellular autophagy is closely related to multiple pathophysiological processes, including aging, neurodegeneration, infectious diseases, immune disorders, and cancer. Autophagy mainly plays a dual role in life and death by regulating cell survival, proliferation, and death, thus being involved in the occurrence, development, and treatment of cancer. It is also involved in chemotherapy resistance by a dual role, since it not only promotes the occurrence of drug resistance but also reverses it. Previous findings suggest that the regulation of autophagy can be used as an effective strategy in tumor therapy. Summary: Recent studies found that small molecules from natural products and their derivatives exert anticancer activity by regulating the level of autophagy in tumor cells. Key Messages: Therefore, this review article describes the mechanism of autophagy, the role of autophagy in normal cells and tumor cells, and the research progress on the anticancer molecular mechanism of targets regulating cell autophagy. The aim is to provide a theoretical basis for developing autophagy inhibitors or activators to improve anticancer efficacy. [ABSTRACT FROM AUTHOR]

Hormonal factors have been suggested to contribute to female dominance among subjects with temporomandibular disorders (TMD). Aim of the study was to examine the association of climacteric status with TMD amongst female participants in the Northern Finland Birth Cohort (NFBC) 1966 at 46 years of age. Among female subjects in NFBC1966, climacteric status was determined based on menstrual anamnesis and measurement of blood follicle-stimulating hormone (FSH) levels. Women with FSH > 25 IU/L and amenorrhoea > 4 months were defined as climacteric (case group, n = 71); women not diagnosed as climacteric were defined as preclimacteric (control group, n = 656). Differences between cases and controls were evaluated on self-reported TMD pain, clinical TMD signs and TMD diagnoses using modified Diagnostic Criteria for TMD (DC/TMD) protocol. Crosstabulation and logistic regression models were used to analyse differences between cases and controls. Compared to preclimacteric women, climacteric women had significantly more often pain on palpation in temporomandibular joints (TMJs) (OR = 2.64, 95% CI 1.12–6.21, p=.026) and more crepitus in TMJs (OR = 2.92, 95% CI 1.13–7.56, p=.027). Degenerative joint disease diagnoses were more common in climacteric than preclimacteric women (OR = 2.27, 95% CI 1.05–4.91, p=.037). Differences were statistically significant after adjusting for confounding factors (body mass index (BMI), smoking, parity). No statistically significant differences in self-reported TMD pain were noted between groups. Among females at the age of 46 years, climacterium seems associated with TMD by increasing pain on palpation in TMJs, subjective symptoms, and clinical signs indicating degenerative changes in TMJs when using DC/TMD. [ABSTRACT FROM AUTHOR]

The genus Groutiella Steere is revised on a worldwide basis. Nine species are accepted: G. apiculata (Hook.) H.A. Crum & Steere, G. chimborazensis (Spruce ex Mitt.) Florsch., G. husnotii (Schimp. ex Besch.) H.A. Crum & Steere, G. macrorrhyncha (Mitt. ex Bosch & Sande Lac.) Wijk & Margad., G. obtusa (Mitt.) Florsch., G. reesei (Vitt) B.H. Allen, G. tomentosa (Hornsch.) Wijk & Margad., G. tuberculata B.H. Allen & I.H. Holz and G. tumidula (Mitt.) Vitt. Four species are reduced to synonymy, Groutiella subgoniorrhyncha (Broth.) Wijk & Margad. and G. thraustophylla (Müll. Hal. ex Broth.) Wijk & Margad. under G. tomentosa, G. maracaibensis (Broth.) B.H. Allen & Goffinet under G. macrorrhyncha, and G. wagneriana (Müll. Hal.) H.A. Crum & Steere under G. apiculata. A key, illustrations and diagnostic features to the recognized species are provided. [ABSTRACT FROM AUTHOR]

Two new furanoeremophilane sesquiterpenoids, namely, 6,9-dioxo-1α,4α-dihydroxy-furanoeremophilane (1) and 4α,5α-epoxy-6,9-dioxo-1α-hydroxyl-furanoeremophilane (2), and 10 known compounds were isolated from the whole plant of Chloranthus multistachys, and compound 3 was converted to derivative 3a. Their structures were determined based on extensive spectroscopic analysis. All compounds were evaluated by using five cancer cell lines: PC3, LNcap, A549, K562, and HEL. The derivative 3a exhibited excellent cytotoxic activities, with the IC50 against HEL cells being the lowest at 1.322 ± 0.08 μM, which was comparable to that of the positive control (doxorubicin). Mechanism studies showed that the anticancer activity of 3a may be associated with cell cycle regulation. [ABSTRACT FROM AUTHOR]

In vivo fluorescence imaging has received extensive attention due to its distinguished advantages of excellent biosafety, high sensitivity, dual temporal‐spatial resolution, real‐time monitoring ability, and non‐invasiveness. Aggregation‐induced emission luminogens (AIEgens) with near‐infrared (NIR) absorption and emission wavelengths are ideal candidate for in vivo fluorescence imaging for their large Stokes shift, high brightness and superior photostability. NIR emissive AIEgens provide deep tissue penetration depth as well as low interference from tissue autofluorescence. Here in this review, we summarize the molecular engineering strategies for constructing NIR AIEgens with high performances, including extending π‐conjugation system and strengthen donor (D)‐acceptor (A) interactions. Then the encapsulation strategies for increasing water solubility and biocompatibility of these NIR AIEgens are highlighted. Finally, the challenges and prospect of fabricating NIR AIEgens for in vivo fluorescence imaging are also discussed. We hope this review would provide some guidelines for further exploration of new NIR AIEgens. [ABSTRACT FROM AUTHOR]

Simple Summary: The addition of carbon sources to white rot fermentation straw can promote the decomposition of organic matter by white rot fungi, shorten the fermentation cycle, and help retain more nutrients in the straw feed. Carbon sources (glucose, sucrose, molasses, or soluble starch) were added in 21 days of fermentation of corn and rice straw by white rot fungus (Phanerocacee pusosporium). The results showed that the molasses and glucose as carbon sources showed the greatest enhancement in the nutritional value of maize and rice straw after 14 days of solid-state fermentation (SSF) treatment. Poor quality straw can be made more digestible and palatable through delignification using white rot fungi as a biological treatment in SSF. The decomposition of organic matter by white rot fungi is improved when a carbon source is added. Reducing the fermentation cycle can also help retain more nutrients in straw feed. To increase rumen digestibility and nutrient utilization, corn straw and rice straw were subjected to SSF with white rot fungi (Phanerochaete chrysosporium) for 21 days. The type of carbon source (glucose, sucrose, molasses, or soluble starch) was optimized, and the nutrient composition and in vitro fermentation parameters of the fermented straw were assessed. In the fermented corn straw and rice straw supplemented with different carbon sources, the results showed a decrease in lignin content, dry matter, cellulose, and hemicellulose loss, and an increase in crude protein content after 21 days. Total volatile fatty acid and ammonium nitrogen concentrations increased significantly (p < 0.01) during in vitro fermentation. Overall, the most enhanced nutritional values for corn straw and rice straw were observed after 14 days of SSF in the groups using molasses or glucose as a carbon source. [ABSTRACT FROM AUTHOR]

Pairing Li-free transition-metal-based cathodes (MX) with Li-metal anodes is an emerging trend to overcome the energy-density limitation of current rechargeable Li-ion technology. However, the development of practical Li-free MX cathodes is plagued by the existing notion of low voltage due to the long-term overlooked voltage-tuning/phase-stability competition. Here, we propose a p-type alloying strategy involving three voltage/phase-evolution stages, of which each of the varying trends are quantitated by two improved ligand-field descriptors to balance the above contradiction. Following this, an intercalation-type 2H-V1.75Cr0.25S4 cathode tuned from layered MX2 family is successfully designed, which possesses an energy density of 554.3 Wh kg−1 at the electrode level accompanied by interfacial compatibility with sulfide solid-state electrolyte. The proposal of this class of materials is expected to break free from scarce or high-cost transition-metal (e.g. Co and Ni) reliance in current commercial cathodes. Our experiments further confirm the voltage and energy-density gains of 2H-V1.75Cr0.25S4. This strategy is not limited to specific Li-free cathodes and offers a solution to achieve high voltage and phase stability simultaneously. [ABSTRACT FROM AUTHOR]