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With the gradual depletion of traditional fossil energy and the increasing demand for electricity, finding new energy sources has become the only way for sustainable development. Microgrid is becoming an important auxiliary power for some developed and developing countries to solve the supply problem of energy system, which can not only effectively improve the overall utilization rate of renewable energy, but also improve the power supply reliability of the main grid. In this paper, the hardware circuit and software control of microgrid simulation system based on STM32 are studied and designed. The main circuit design of microgrid simulation system is based on STM32 single chip microcomputer as the main control core, and the pulse modulation signal SPWM is output through the software program of STM32F103CBT6 single chip microcomputer. The MOS driving circuit provides driving voltage and control signal to drive the inverter bridge to invert DC into AC. The inverter bridge is modulated by SPWM control technology, and the high-frequency signal in AC is filtered by low-pass filtering circuit, and finally the stable AC with a fixed frequency of 50 Hz is the output. By calculating the device parameters of the main control circuit and the full-bridge inverter circuit in the hardware unit circuit, and making appropriate selection of components, the specific model is determined, and finally the circuit design of the whole circuit is completed. The hardware circuit is designed rationally by Altium designer, and the software circuit is programmed by Keil. The influence of SPWM signal output by the circuit on the resistance load and its output waveform is analyzed comprehensively, and the influence of working frequency on the output waveform of the circuit is studied.

To analyze the expression of pro-platelet basic protein and transcription factor activating enhancer binding protein 4 genes in non-small cell lung cancer and the prognostic value. 94 patients with non-small cell lung cancer undergoing lung cancer resection in our hospital from January 2015 to January 2016 were selected as study subjects and pro-platelet basic protein and transcription factor activating enhancer binding protein 4 messenger RNA expressions in cancer tissues and paracancerous tissues were detected by immunohistochemistry and other methods. The relationship between different levels of pro-platelet basic protein and transcription factor activating enhancer binding protein 4 messenger RNA expression levels and clinicopathological characteristics of patients was analyzed using Kaplan-Meier curves, used for survival analysis. Transcription factor activating enhancer binding protein 4 was lower in non-small cell lung cancer tissues than in paraneoplastic tissues (p

In order to improve the security of economic financial management data storage, a multi-level distributed storage encryption algorithm of economic financial management database based on piecewise fusion encryption and information clustering is proposed. Under the arithmetic coding key encryption system, the data distributed structure model of economic financial management data storage is constructed, and the encrypted statistical characteristics of economic financial management data are extracted. The homomorphism data fusion method is used to deal with the fuzzy clustering of economic and financial management data, and the fuzzy C-means clustering method is combined to carry out the piecewise fusion scheduling of economic and financial management data. The chaotic arithmetic coding scheme is used to design the economic financial management data storage encryption in the segmented interval, and the encryption key and decryption key are constructed based on piecewise fusion encryption and information clustering. Realize the multi-level distributed storage design of economic financial management database. The simulation results show that the distributed storage and encryption design of economic financial management data is well integrated, the ability of anti-deciphering is strong, and the security of economic and financial management data is improved.

BACKGROUND: This study aims to investigate the effects of education with health belief model (HBM) on anxiety and fatigue among patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). METHODS: Patients with AECOPD admitted into Taizhou People’s Hospital, Jiangsu, China between December 2012 and October 2013 were randomly selected for the present study according to random number table. A total of 99 cases with anxiety were included. These patients were divided into two groups: experimental group educated by HBM (n=47), and control group educated by conventional method (n=52). The scores for anxiety and fatigue were evaluated using the self-rating anxiety scale (SAS) and multidimensional fatigue inventory (MFI-20). RESULTS: After educational intervention by HBM, patients achieved a significant decrease in anxiety and fatigue scores, when compared to patients in the control group, who were educated by the conventional method at the time of admission, discharge, and 6 weeks after discharge (P

The Zhibula Cu skarn deposit contains 0.32 Mt. Cu metal with an average grade of 1.64% and is located in the Gangdese porphyry copper belt in southern Tibet. The deposit is a typical metasomatic skarn that is related to the interaction of magmatic–hydrothermal fluids and calcareous host rock. Stratiform skarn orebodies occur at the contact between tuff and marble in the Lower Jurassic Yeba Formation. Alteration zones generally grade from a fresh tuff to a garnet-bearing tuff, a garnet pyroxene skarn, and finally to a wollastonite marble. Minor endoskarn alteration zonations are also observed in the causative intrusion, which grade from a fresh granodiorite to a weakly chlorite-altered granodiorite, a green diopside-bearing granodiorite, and to a dark red-brown garnet-bearing granodiorite. Prograde minerals, which were identified by electron probe microanalysis include andradite–grossularite of various colors (e.g., red, green, and yellow) and green diopside. Retrograde metamorphic minerals overprint the prograde skarn, and are mainly composed of epidote, quartz, and chlorite. The ore minerals consist of chalcopyrite and bornite, followed by magnetite, molybdenite, pyrite, pyrrhotite, galena, and sphalerite. Three types of fluid inclusions are recognized in the Zhibula deposit, including liquid-rich two-phase inclusions (type L), vapor-rich two-phase inclusions (type V), and daughter mineral-bearing three-phase inclusions (type S). As the skarn formation evolved from prograde (stage I) to early retrograde (stage II) and later retrograde (stage III), the ore-forming fluids correspondingly evolved from high temperature (405–667 °C), high salinity (up to 44.0 wt.% NaCl equiv.), and high pressure (500–600 bar) to low-moderate temperature (194–420 °C), moderate-high salinity (10.1–18.3 and 30.0–44.2 wt.% NaCl equiv.), and low-moderate pressure (250–350 bar). Isotopic data of δ 34 S (− 0.1‰ to − 6.8‰, estimated δ 34 S fluids = − 0.7‰), δD H2O (− 91‰ to − 159‰), and δ 18 O H2O (1.5‰ to 9.2‰) suggest that the ore-forming fluid and material came from magmatic–hydrothermal fluids that were associated with Miocene Zhibula intrusions. Fluid immiscibility likely occurred at the stage I and stage II during the formation of the skarn and mineralization. Fluid boiling occurred during the stage III, which is the most important Cu deposition mechanism for the Zhibula deposit.

The Zhibula Cu skarn deposit (19.5 Mt ore @ 1.64%), near the Qulong porphyry Cu–Mo deposit, is located in the Gangdese porphyry copper belt in southern Tibet. The deposit is a typical metasomatic skarn that is related to the interaction of magmatic-hydrothermal fluids and calcareous host rocks. Stratiform skarn orebodies are mainly distributed in the contact between tuff and marble in the lower part of the Jurassic Yeba Formation. Endoskarn zonations for an outward trend are observed in the granodiorite, which grade from a fresh granodiorite to a weakly chlorite-altered granodiorite, a green diopside-bearing granodiorite, and a dark red-brown garnet-bearing granodiorite. The Zhibula granodiorite and monzogranite have similar secondary ion mass spectrometry (SIMS) zircon U–Pb ages of 16.9 ± 0.3 Ma and 17.0 ± 0.2 Ma, respectively. They exhibit different fractional crystallization from granodiorite (SiO 2 = 64.8–69.3 wt.%) to monzogranite (SiO 2 = 72.3–76.8 wt.%). Both the granodiorite and monzogranite are characterized by high Al 2 O 3 (12.6–16.7 wt.%) and K 2 O (1.5–5.5 wt.%) contents, high Sr/Y (35–151) and La/Yb (19–48) ratios, and variable MgO (0.16–3.91) and Mg# (31–61) values. They display features of enrichment in large ion lithophile elements (LILEs, e.g., Rb, Ba, Sr, and K), depletion in high field strength elements (HFSEs, e.g., Nb, Ta, Ti, and P), and moderate negative Eu anomalies (δEu = 0.58–0.98). They show restricted in situ zircon Hf isotopic compositions (+6.7 to +8.8; only one sample is +4.5) and consistent δ 18 O values (+6.0‰ to +6.6‰). The geochemical data indicated that the Miocene Zhibula granitic intrusions formed by the magma that were characterized by high Sr/Y ratios and were derived from the partial melting of the thickened juvenile lower crust, which may have been metasomatized by the slab melts during subduction of the Neo-Tethyan oceanic crust and were induced by the convective removal of the thickened lithosphere. In addition, the Zhibula Cu skarn deposit and the Qulong Cu–Mo deposit form a significant porphyry–skarn ore system.

Accumulating evidence has revealed that the mammalian heart possesses a measurable capacity for renewal. Neonatal mice retain a regenerative capacity over a short time-frame (≤6 days), but this capacity is lost by 7 days of age. In the present study, differential gene expression profiling of mouse cardiac tissue was performed to further elucidate the mechanisms underlying this process. The global gene expression patterns of the neonatal C57BL/6J mouse heart were examined at three key time-points (1, 6 and 7 days old) using digital gene expression analysis. In the distribution of total clean tags, high-expression tags (>100 copies) were found to be predominant, whereas low expression tags (

Long noncoding RNAs (lncRNAs) represent a sub-group of noncoding RNAs that are longer than 200 nucleotides. The characterization of lncRNAs and their acceptance as crucial regulators of numerous developmental and biological pathways have suggested that the lncRNA study has gradually become one of the hot topics in the field of RNA biology. Many lncRNAs show spatially and temporally restricted expression patterns during embryogenesis and organogenesis. This study aimed to characterize the lncRNA profile of the fetal mouse heart at three key time points (embryonic day E11.5, E14.5, and E18.5) in its development, by performing a microarray lncRNAs screen. Gene Ontology analysis and ingenuity pathway analysis showed some significant gene functions and pathways were altered in heart development process. We compared lncRNAs profile between the three points (E14.5 vs. E11.5 [early development]; E18.5 vs. E14.5 [later development]). A total of 1,237 lncRNAs were found to have consistent fold changes (>2.0) between the three time points. Among them, 20 dysregulated lncRNAs were randomly selected and confirmed by real-time qRT-PCR. Additionally, bioinformatics analysis of AK011347 suggested it may be involved in heart development through the target gene Map3k7. In summary, this study identified differentially expressed lncRNAs in the three time points studied, and these lncRNAs may provide a new clue of mechanism of normal heart development.

Long noncoding RNAs (lncRNAs) represent a sub-group of noncoding RNAs that are longer than 200 nucleotides. The characterization of lncRNAs and their acceptance as crucial regulators of numerous developmental and biological pathways have suggested that the lncRNA study has gradually become one of the hot topics in the field of RNA biology. Many lncRNAs show spatially and temporally restricted expression patterns during embryogenesis and organogenesis. This study aimed to characterize the lncRNA profile of the fetal mouse heart at three key time points (embryonic day E11.5, E14.5, and E18.5) in its development, by performing a microarray lncRNAs screen. Gene Ontology analysis and ingenuity pathway analysis showed some significant gene functions and pathways were altered in heart development process. We compared lncRNAs profile between the three points (E14.5 vs. E11.5 [early development]; E18.5 vs. E14.5 [later development]). A total of 1,237 lncRNAs were found to have consistent fold changes (2.0) between the three time points. Among them, 20 dysregulated lncRNAs were randomly selected and confirmed by real-time qRT-PCR. Additionally, bioinformatics analysis of AK011347 suggested it may be involved in heart development through the target gene Map3k7. In summary, this study identified differentially expressed lncRNAs in the three time points studied, and these lncRNAs may provide a new clue of mechanism of normal heart development.

Fatty acid-binding protein 3 (FABP3) is a low-molecular-weight protein with a distinct tissue distribution that may play an important role in fatty acid transport, cell growth, cellular signaling, and gene transcription. Previously, we have found that FABP3 was involved in apoptosis-associated congenital cardiac malformations, but the underlying mechanisms have not yet been described. In the present study, we investigated the characteristics of mitochondrial dysfunction in embryonic cancer cells (P19 cells) that overexpressed FABP3. We demonstrated that in FABP3-overexpressing P19 cells a lower cellular ATP production was accompanied by a dramatic decrease in mitochondrial membrane potential (MMP), despite the lack of a substantial decrease in the mtDNA copy number. In addition, FABP3 overexpression also led to an imbalance in mitochondrial dynamics and to excess intracellular reactive oxygen species production. Collectively, our results indicated that overexpression of FABP3 in P19 cells caused mitochondrion dysfunction that might be responsible for the development of FABP3-induced apoptosis.

NYGGF4 is a recently identified gene that is involved in obesity-associated insulin resistance. Previous data from this laboratory have demonstrated that NYGGF4 overexpression might contribute to the development of insulin resistance (IR) and to mitochondrial dysfunction. Additionally, NYGGF4 knockdown enhanced insulin sensitivity and mitochondrial function in 3T3-L1 adipocytes. We designed this study to determine whether silencing of NYGGF4 in 3T3-L1 adipocytes could rescue the effect of insulin sensitivity and mitochondrial function induced by the cyanide p-trifluoromethoxyphenyl-hydrazone (FCCP), a mitochondrion uncoupler, to ascertain further the mechanism of NYGGF4 involvement in obesity-associated insulin resistance. We found that 3T3-L1 adipocytes, incubated with 5μM FCCP for 12h, had decreased levels of insulin-stimulated glucose uptake and had impaired insulin-stimulated GLUT4 translocation. Silencing also diminished insulin-stimulated tyrosinephosphorylation of IRS-1 and serine phosphorylation of Akt. This phenomenon contrasts with the effect of NYGGF4 knockdown on insulin sensitivity and describes the regulatory function of NYGGF4 in adipocytes insulin sensitivity. We next analyzed the mitochondrial function in NYGGF4-silenced adipocytes incubated with FCCP. NYGGF4 knockdown partly rescued the dissipation of mitochondrial mass, mitochondrial DNA, intracellular ATP synthesis, and intracellular reactive oxygen species (ROS) production occurred following the addition of FCCP, as well as inhibition of mitochondrial transmembrane potential (ΔΨm) in 3T3-L1 adipocytes incubated with FCCP. Collectively, our results suggested that addition of silencing NYGGF4 partly rescued the effect of insulin resistance and mitochondrial dysfunction in NYGGF4 silenced 3T3-L1 adipocytes incubated with FCCP, which might explain the involvement of NYGGF4-induced IR and the development of NYGGF4 in mitochondrial function.