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2 results on '"Ya-Qian Duan"'

1. Neutrophil extracellular traps facilitate liver inflammation/fibrosis progression by entering macrophages and triggering AIM2 inflammasome-dependent pyroptosis

Author

Yu Zhang, Rong Wu, Xi Zhan, Xuan-Yi Wang, Lin-Wei Xiang, Ya-Qian Duan, Yan You, Jian-Bo Zhang, Rui Wu, Yun-Yuan Zhang, and Liang Duan

Subjects
AIM2 inflammasome, Pyroptosis, Neutrophil extracellular traps, Chronic liver inflammation/fibrosis, Medicine, Cytology, QH573-671
Abstract

Abstract Background Absent in melanoma 2 (AIM2) inflammasome-dependent pyroptosis and neutrophil extracellular traps (NETs) have been implicated in chronic liver disease (CLD). However, the specific intrahepatic cell type that undergoes AIM2 inflammasome-dependent pyroptosis and how their interaction augments hepatic inflammation/fibrosis remains unclear. Methods The expression and correlation of AIM2 inflammasome-dependent pyroptosis-related indicators and NETs were analyzed in biopsy tissue and blood specimens from chronic hepatitis patients (CHs). Cell-based experiments were conducted to investigate their interaction. In vitro and in vivo experiments were used to analyze their effects on the progression of hepatic inflammation/fibrosis as well as their clinical importance. Results Elevated levels of AIM2 inflammasome-dependent pyroptosis indicators and NETs were detected in biopsy tissue and blood specimens. Circulating NETs were positively correlated with pyroptosis-related indicators, and both were related with disease severity. Confocal imaging revealed that AIM2 was mainly localized to hepatic macrophages, indicating that hepatic macrophages were the major cell type that underwent pyroptosis. NETs were directly engulfed by macrophages and then stimulated AIM2 inflammasome-dependent macrophage pyroptosis in vitro, which amplified the activation of hepatic stellate cells (HSCs) and increased collagen deposition. Administration of the NETs degradation agent DNase I or the AIM2 inflammasome activation inhibitor ODN A151 effectively alleviated chronic liver inflammation/fibrosis progression in vivo. Conclusions NETs-induced AIM2 inflammasome-dependent pyroptosis in macrophages facilitates liver inflammation/fibrosis progression. The identified NET–AIM2 inflammasome cascade could serve as a novel therapeutic target for hepatic inflammation/fibrosis progression.

Published
2024
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2. Convergence role of transcriptional coactivator p300 and apparent modification on HMCs metabolic memory induced by high glucose

Author

Hong SU, Bo ZHOU, Ya-qian DUAN, and Chao DU

Subjects
lcsh:R5-920, p300-CBP transcription factors, lcsh:R, oxidative stress, lcsh:Medicine, metabolic memory, glycosylation end products, advanced, lcsh:Medicine (General), protein kinase C
Abstract

Objective To investigate the protein expression of transcriptional coactivator p300, acetylated histone H3 (Ac-H3) and Ac-H4 in human renal mesangial cell (HMCs) as imitative "metabolic memory" in vitro, and explore the potential role of convergence point of p300. Methods The HMCs were divided into the following groups: ① High glucose metabolic memory model: normal glucose group (NG, 5.5mmol/L D-glucose×2d), high glucose group (HG, 25mmol/L D-glucose×2d), memory groups (M1, M2, M3, 25mmol/L D-glucose×2days + 5.5mmol/L D-glucose×3d, 6d or 9d), persisting normal glucose group (NG, 5.5mmol/L D-glucose×9d). ② Advanced glycation end products memory model: normal glucose group (NG, 5.5mmol/ L D-glucose×2d), NG+AGEs group (AGEs, 5.5mmol/L D-glucose+250µg/ml AGEs×2d); AGEs memory group (AGEs-M, 5.5mmol/L D-glucose + 250µg/ml AGEs×2d + 5.5mmol/L D-glucose×3d); BSA control group (NG+BSA, 5.5mmol/L D-glucose + 250µg/ml BSA×2d). ③ H2O2 was used to simulate oxidative stress memory model: normal glucose group (NG, 5.5mmol/L D-glucose×2d), NG+H2O2 group (H2O2, 5.5mmol/L D-glucose +100µmol/L H2O2×30min); H2O2 memory group [(5.5mmol/ L D-glucose + 100µmol/L H2O2×30min) + 5.5mmol/L D-glucose×3d]; normal glucose control group (NG3, 5.5mmol/L D-glucose×3d). ④ Transfection with PKCβ2 memory model: normal glucose group (NG, 5.5mmol/L D-glucose×2d); high glucose group (HG, 25mmol/L D-glucose×2d); memory group (M, 25mmol/L D-glucose×2d + 5.5mmol/L D-glucose×3d); Ad5-null memory group (HN, 25mmol/L D-glucose + Ad5-null×2d + 5.5mmol/L D-glucose×3d); PKCβ2 memory group (PO, 25mmol/L D-glucose + Ad5-PKCβ2×2d + 5.5mmol/L D-glucose×3d); inhibitor of PKCβ2 memory group (PI, 25mmol/L D-glucose×2d + 10µmol/L CGP53353 + 5.5mmol/L D-glucose×3d). The expression of intracellular reactive oxygen species (ROS) was detected by fluorescence microscope and fluorescence microplate reader. The expression levels of p300, Ac-H3, Ac-H4 and PKCβ2 proteins were determined by Western blotting. Results The expression levels of p300, Ac-H3 and Ac-H4 protein in HG group increased, being 2.15, 1.93 and 1.87 fold of those in group NG (P

Published
2013

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