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In radio astronomy observations, radio frequency interference (RFI) is mixed into the telescope receiving system in various forms. The existence of RFI brings misjudgment to the observation or reduces the observational signal-to-noise ratio. In recent years, the domestic and overseas radio astronomy has developed rapidly. Large-scale radio telescopes and telescope arrays at home and abroad have been constructed successively. Observation sensitivity is greatly improved, and the influence of RFI is particularly prominent. With the development of science and technology and the intensification of human activities, the RFI has become increasingly serious and irreversible. We propose to use the two-dimensional discrete wavelet transform method to analyze the data of radio astronomy observations, and to perform wavelet transform on the time-frequency sequence output by the telescope system. According to the wavelet coefficients, the every component in the original signal is separated, and the corresponding threshold value is obtained by the statistics on each component. Each component is compared with the threshold to identify the interference component, and to mark it for removal. This method is used to process the actual observation data. The results show that this method can well mark and eliminate the interference signal, and improve the observational signal-to-noise ratio. [ABSTRACT FROM AUTHOR]

In order to carry out the spectral observation with the TM65m radio telescope, the frequency calibration and test of DIBAS (Digital Backend System) are performed, it is found that it has a good performance. First, by injecting the PCAL signals, the frequency resolution, frequency drift and the stability of frequency interval between two spectral lines of the DIBAS backend are measured. It is found that in one hour, the maximum frequency drift of a single spike is 0.03 channel, the maximum fluctuation of spike interval is 0.05 channel. Then, by the observations on the H 2 CO maser and absorbtion lines of massive star formation regions, and the comparison with the results observed by the GBT (Robert C. Byrd Green Bank Telescope), it is shown that the results of frequency calibration are correct. Finally, by the OH maser observations in more than one hour toward W3(OH), and the methanol maser observations in more than 5 hours, it is found that the spectral profiles keep consistent, and the observational noise is consistent with the theoretical value, indicating the stability and reliability of the frequency calibration program. [ABSTRACT FROM AUTHOR]

Describes the development of a polymerase chain reaction method based on amplification of a specific DNA fragment for the identification of camel, Camelus materials. Design of a species-specific primer pair L183/H372 based no the nucleotide sequence of the mitochondrial cytochrome b gene; Amplification of a pair fragment.

The lunar rendezvous and docking (RVD) technology is an important guarantee for our third phase of lunar exploration. In this paper, by using VLBI (Very Long Baseline Interferometry) delay of CE03 (Chang'e-03) which was around the lunar, the changing of differential group delay (DGD) under the SBI (same-beam VLBI) observation mode was simulated. The simulation results indicated that the orbit distance between Orbiter and Ascender Combination (Ascender and Returner) was kept in 100 kilometers, and it lasted for half an hour, the track information of these two were well reflected by DGD which can be used for orbit determination. In the approach stage, DGD is rapidly approaching to 0, as Ascender Combination accelerated and closed to the orbiter, while the orbit distance was reduced from 5 km to 20 m, and was rapidly increased as Ascender Combination was far away from Orbiter. At last, using VLBI delay of CE03 which was halfway to the lunar, and transmitted two DOR (Differential One-Ranging) signals that were observed by CVN (Chinese VLBI Network), we calculated DPD (Differential Phase Delay), and preliminarily showed the error of DPD. [ABSTRACT FROM AUTHOR]

Emission geometries, including emission region heights, beam shapes and radius-to-frequency mapping, are important clues for pulsar radiation models. Multiband radio observations reveal this valuable information. In this paper, we study two bright pulsars, PSRs B0329+54 and B1642-03, and observe them at high frequencies: 2.5, 5 and 8 GHz. The newly acquired data, together with historical archives, provide an atlas of multifrequency profiles spanning from 100 MHz to 10 GHz. We study the frequency evolution of pulse profiles as well as radiation regions using these data. First, we fit the pulse profiles with Gaussian functions to determine the phase of each component, and then we calculate the radiation altitudes of different emission components and radiation regions. We find that the inverse Compton scattering (ICS) model can reproduce the radiation geometry of these two pulsars. However, for PSR B0329+54, the radiation can be generated in either an annular gap (AG) or a core gap (CG), while the radiation of PSR B1642-03 can only be generated in a CG. This difference is caused by the inclination angle and the impact angle of these two pulsars. The relationship between the beaming angle (the angle between the radiation direction and the magnetic axis) and the radiation altitude versus frequency is also presented by modelling the beam–frequency evolution in the ICS model. The multiband pulse profiles of these two pulsars can be described well by the ICS model combined with the CG and AG. [ABSTRACT FROM AUTHOR]

We report the results of the OH maser observation towards the water fountain source IRAS 18286–0959 using the newly built Shanghai Tianma 65-m Radio Telescope. We observed the three OH ground state transition lines at frequencies of 1612, 1665 and 1667 MHz. Comparing with the spectra of previous observations, we find new maser spectral components at velocity channels largely shifted from the systemic velocity: the velocity offsets of the newly found components lie in the range 20-40 km s-1 with respect to the systemic velocity. Besides maser variability, another possible interpretation for the newly detected maser features is that part of the molecular gas in the circumstellar envelope is accelerated. The acceleration is probably caused by the passage of a high-velocity molecular jet, which has been detected in previous Very Long Baseline Interferometry observations in the H2O maser line. [ABSTRACT FROM AUTHOR]

On 14th December 2013, the Chang’E-3 made a successful soft landing on the lunar surface, and then carried out the tasks of separating the lander and the rover, and taking pictures of each other. With the same beam VLBI (Very Long Baseline Interferometry) technique to observe the signals transmitted by the lander and the rover simultaneously, the differential phase delay between them is calculated, which can reflect the minor changes of the rover's position on a scale of a few centimeters. Based on the high sensitivity of differential phase delay, the rover's speeds during 5 movements are obtained with an average of 0.056 m/s. The relationship between the rover's shake in the moving process and the lunar terrain is analyzed by using the spectrum of the residual of the differential phase delay after the first-order polynomial fitting. [ABSTRACT FROM AUTHOR]

Genetic alterations which impair the function of the TP53 signaling pathway in TP53 wild-type human tumors remain elusive. To identify new components of this pathway, we performed a screen for genes whose loss-of-function debilitated TP53 signaling and enabled oncogenic transformation of human mammary epithelial cells. We identified transglutaminase 2 (TGM2) as a putative tumor suppressor in the TP53 pathway. TGM2 suppressed colony formation in soft agar and tumor formation in a xenograft mouse model. The depletion of growth supplements induced both TGM2 expression and autophagy in a TP53-dependent manner, and TGM2 promoted autophagic flux by enhancing autophagic protein degradation and autolysosome clearance. Reduced expression of both CDKN1A, which regulates the cell cycle downstream of TP53, and TGM2 synergized to promote oncogenic transformation. Our findings suggest that TGM2-mediated autophagy and CDKN1A-mediated cell cycle arrest are two important barriers in the TP53 pathway that prevent oncogenic transformation. [ABSTRACT FROM AUTHOR]

The lipotoxicity hypothesis posits that obesity predisposes individuals to metabolic diseases because the oversupply of lipids to tissues not suited for fat storage leads to the accumulation of fat-derived molecules that impair tissue function. Means of combating this have been to stimulate anabolic processes to promote lipid storage or to promote catabolic ones to drive fat degradation. Herein, we demonstrate that ablating dihydroceramide desaturase 1 (Des1), an enzyme that produces ceramides, leads to the simultaneous activation of both anabolic and catabolic signaling pathways. In cells lacking DesI, the most common sphingolipids were replaced with dihydro forms lacking the double bond inserted by Des1. These cells exhibited a remarkably strong activation of the antiapoptotic and anabolic signaling pathway regulated by Akt/protein kinase B (PKB), were resistant to apoptosis, and were considerably larger than their wild-type counterparts. Paradoxically, Des1-/- cells exhibited high levels of autophagy. Mechanistic studies revealed that this resulted from impaired ATP synthesis due in part to decreased expression and activity of several complexes of the electron transport chain, particularly complex IV, leading to activation of AMP-activated protein kinase and its induction of the autophagosome. Thus, Des1 ablation enhanced starvation responses but dissociated them from the anabolic, prosurvival, and antiautophagic Akt/PKB pathways. [ABSTRACT FROM AUTHOR]

We have investigated the mode changing properties of PSR B0329+54 using 31 epochs of simultaneous 13 cm/3 cm single-pulse observations obtained with the Shanghai Tian Ma 65 m telescope. The pulsar was found in the abnormal emission mode 17 times, accounting for ∼13% of the 41.6 hr total observation time. Single-pulse analyses indicate that mode changes took place simultaneously at 13 cm/3 cm within a few rotational periods. We detected occasional bright and narrow pulses whose peak flux densities were 10 times higher than that of the integrated profile in both bands. At 3 cm, about 0.66% and 0.27% of single pulses were bright in the normal mode and abnormal mode, respectively, but at 13 cm the occurrence rate was only about 0.007%. We divided the pulsar radiation window into three components (C1, C2, and C3) corresponding to the main peaks of the integrated profile. The bright pulses preferentially occurred at pulse phases corresponding to the peaks of C2 and C3. Fluctuation spectra showed that C2 had excess red noise in the normal mode, but broad quasi-periodic features with central frequencies around 0.12 cycles/period in the abnormal mode. At 3 cm, C3 had a stronger quasi-periodic modulation centered around 0.06 cycles/period in the abnormal mode. Although there were some asymmetries in the two-dimensional fluctuation spectra, we found no clear evidence for systematic subpulse drifting. Consistent with previous low-frequency observations, we found a very low nulling probability for B0329+54, with upper limits of 0.13% and 1.68% at 13 cm/3 cm, respectively. [ABSTRACT FROM AUTHOR]

We performed a systematic 6.7 GHz Class II methanol maser survey using the Shanghai Tianma Radio Telescope toward targets selected from the all-sky Wide-Field Infrared Survey Explorer (WISE) point catalog. In this paper, we report the results from the survey of those at high Galactic latitudes, i.e., 2°. Of 1473 selected WISE point sources at high latitude, 17 point positions that were actually associated with 12 sources were detected with maser emission, reflecting the rarity (1%–2%) of methanol masers in the region away from the Galactic plane. Out of the 12 sources, 3 are detected for the first time. The spectral energy distribution at infrared bands shows that these new detected masers occur in the massive star-forming regions. Compared to previous detections, the methanol maser changes significantly in both spectral profiles and flux densities. The infrared WISE images show that almost all of these masers are located in the positions of the bright WISE point sources. Compared to the methanol masers at the Galactic plane, these high-latitude methanol masers provide good tracers for investigating the physics and kinematics around massive young stellar objects, because they are believed to be less affected by the surrounding cluster environment. [ABSTRACT FROM AUTHOR]

Integrated pulse profiles at 8.6 GHz obtained with the Shanghai Tian Ma Radio Telescope (TMRT) are presented for a sample of 26 pulsars. Mean flux densities and pulse width parameters of these pulsars are estimated. For 11 pulsars these are the first high-frequency observations and for a further four, our observations have a better signal-to-noise ratio than previous observations. For one (PSR J0742−2822), the 8.6 GHz profile differs from previously observed profiles. A comparison of 19 profiles with those at other frequencies shows that in nine cases, the separation between the outmost leading and trailing components decreases with frequency, which is roughly in agreement with radius-to-frequency mapping, whereas in the other 10 the separation is nearly constant. Different spectral indices of profile components lead to the variation of integrated pulse profile shapes with frequency. In seven pulsars with multi-component profiles, the spectral indices of the central components are steeper than those of the outer components. For the 12 pulsars with multi-component profiles in the high-frequency sample, we estimate the core width using Gaussian fitting and discuss the width-period relationship. [ABSTRACT FROM AUTHOR]

We report Shanghai Tian Ma Radio Telescope (TMRT) detections of several long carbon-chain molecules in the C and Ku bands, including HC3N, HC5N, HC7N, HC9N, C3S, C6H, and C8H toward the starless cloud Serpens South 1a. We detected some transitions (HC9N J = 13–12, F = 12–11, and F = 14–13; H13CCCN J = 2–1, F = 1–0, and F = 1–1; HC13CCN J = 2–1, F = 2–2, F = 1–0, and F = 1–1; HCC13CN J = 2–1, F = 1–0, and F = 1–1) and resolved some hyperfine components (HC5N J = 6–5, F = 5–4; H13CCCN J = 2–1, F = 2–1) for the first time in the interstellar medium. The column densities of these carbon-chain molecules in the range 1012–1013 cm−2 are comparable to two carbon-chain molecule rich sources, TMC-1 and Lupus-1A. The abundance ratios are 1.00:(1.11 ± 0.15):(1.47 ± 0.18) for [H13CCCN]:[HC13CCN]:[HCC13CN]. This result implies that the 13C isotope is also concentrated in the carbon atom adjacent to the nitrogen atom in HC3N in Serpens South 1a, which is similar to TMC-1. The [HC3N]/[H13CCCN] ratio of 78 ± 9, the [HC3N]/[HC13CCN] ratio of 70 ± 8, and the [HC3N]/[HCC13CN] ratio of 53 ± 4 are also comparable to those in TMC-1. Serpens South 1a proves to be a suitable testing ground for understanding carbon-chain chemistry. [ABSTRACT FROM AUTHOR]

In this paper, we report radio observations of the Galactic Center magnetar PSR J1745–2900 at six epochs between 2014 June and October. These observations were carried out using the new Shanghai Tian Ma Radio Telescope at a frequency of 8.6 GHz. Both the flux density and integrated profile of PSR J1745–2900 show dramatic changes from epoch to epoch, showing that the pulsar was in its “erratic” phase. On MJD 56836, the flux density of this magnetar was about 8.7 mJy, which was 10 times larger than that reported at the time of discovery, enabling a single-pulse analysis. The emission is dominated by narrow “spiky” pulses that follow a log-normal distribution in peak flux density. From 1913 pulses, we detected 53 pulses whose peak flux densities are 10 times greater than that of the integrated profile. They are concentrated in pulse phase at the peaks of the integrated profile. The pulse widths at the 50% level of these bright pulses were between 0.°2 and 0.°9, much narrower than that of the integrated profile (∼12°). The observed pulse widths may be limited by interstellar scattering. No clear correlation was found between the widths and peak flux density of these pulses and no evidence was found for subpulse drifting. Relatively strong spiky pulses are also detected in the other five epochs of observation, showing the same properties as those detected in MJD 56836. These strong spiky pulses cannot be classified as “giant” pulses but are more closely related to normal pulse emission. [ABSTRACT FROM AUTHOR]