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876 results on '"Yaguchi, Takashi"'

19. Intractable Nocardial mycetoma with possible colonisation by Candida species.

Author

Sumioki, Ayano, Saito, Kanami, Nishida, Haruto, Nishizono, Akira, Miyamoto, Yuji, Ishii, Norihisa, Hiruma, Masataro, Shibuya, Kazutoshi, Yaguchi, Takashi, and Hatano, Yutaka

Abstract

A Japanese male in his 30s with no underlying medical condition presented with painless nodules after being bitten by a dog during a stay in Bali, Indonesia, 7 years earlier. He was referred to our department with multiple ulcers, nodules, and masses on the right leg. The final diagnosis was mycetoma caused by Nocardia vulneris, which may have been exacerbated by colonization of Candida parapsilosis and C. tropicalis as these yeasts were isolated by culture from the tissue. Treatment with minocycline hydrochloride and sulfamethoxazole trimethoprim showed partial efficacy, but the addition of posaconazole achieved significant efficacy. This suggests that the surmised coexistence of pathogenic yeasts of lower virulency may have made mycetoma in this case intractable. [ABSTRACT FROM AUTHOR]

Published
2024
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26. The emergence of rare nocardiosis following allogeneic hematopoietic stem cell transplantation in the era of molecular taxonomy

Author

Kurosawa, Shuhei, Sekiya, Noritaka, Doki, Noriko, Yaguchi, Takashi, Kishida, Yuya, Nagata, Akihito, Yamada, Yuta, Konishi, Tatsuya, Kaito, Satoshi, Yoshifuji, Kota, Shirane, Shuichi, Uchida, Tomoyuki, Inamoto, Kyoko, Toya, Takashi, Igarashi, Aiko, Najima, Yuho, Muto, Hideharu, Kobayashi, Takeshi, Kakihana, Kazuhiko, Sakamaki, Hisashi, and Ohashi, Kazuteru

Published
2019
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28. Rapidly progressive mucus plugs in allergic bronchopulmonary mycosis

Author

Miyazaki, Osamu, primary, Igarashi, Akira, additional, Sato, Kento, additional, Inoue, Sumito, additional, Yokoyama, Ryuto, additional, Nakane, Masaki, additional, Kodama, Sahoko, additional, Hasegawa, Ryo, additional, Ueki, Shigeharu, additional, Yaguchi, Takashi, additional, Watanabe, Akira, additional, and Watanabe, Masafumi, additional

Published
2024
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36. A case of cutaneous chromoblastomycosis in a toddler due to Verticillium spp. identified by molecular biological methods from formalin‐fixed and paraffin‐embedded samples

Author

Yamada, Izumi, primary, Kakuta, Risa, additional, Sato, Tomotaka, additional, Yamazaki, Kazuto, additional, Yaguchi, Takashi, additional, Kitahara, Hirokazu, additional, Takae, Yujiro, additional, Nakamura, Yoshio, additional, Amagai, Masayuki, additional, and Tanikawa, Akiko, additional

Published
2023
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42. Successful treatment by switching from benralizumab to dupilumab in a patient with allergic bronchopulmonary mycosis caused by Schizophyllumcommune

Author

Motohashi, Takumi, Sekiya, Muneyuki, Sakamoto, Susumu, Fukuda, Kohshi, Nakamura, Yasuhiko, Sasaki, Masakazu, Tochigi, Naobumi, Yaguchi, Takashi, Kamei, Katsuhiko, and Kishi, Kazuma

Abstract

A 79-year-old female patient with a history of bronchial asthma was diagnosed with allergic bronchopulmonary aspergillosis. Her symptoms temporarily improved with inhaled corticosteroid/long-acting β2-agonist therapy. However, 10 months after treatment, her asthma symptoms worsened. Sputum culture showed white cotton wool colonies, which were identified as Schizophyllum communevia matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and gene sequencing. The patient tested positive for S. commune-specific IgG. Thus, she was diagnosed with Schizophyllum commune-induced allergic bronchopulmonary mycosis.The patient was initially treated with benralizumab. However, it was not effective. After switching to dupilumab, her symptoms and mucus plugs improved.

Published
2025
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47. Specific and sensitive loop-mediated isothermal amplification (LAMP) method for Madurella strains, eumycetoma filamentous fungi causative agent

Author

Yoshioka, Isato, Mori, Yugo, Fahal, Ahmed Hassan, Siddig, Emmanuel Edwar, Kaneko, Satoshi, Yaguchi, Takashi, Yoshioka, Isato, Mori, Yugo, Fahal, Ahmed Hassan, Siddig, Emmanuel Edwar, Kaneko, Satoshi, and Yaguchi, Takashi

Abstract

Background Filamentous fungi of the genus Madurella are the primary causative agents of mycetoma, a disease observed in tropical and subtropical regions. Since early diagnostics based on a morphological approach are difficult and have many shortcomings, a molecular diagnostic method suitable for rural settings is required. In this study, we developed the loop-mediated isothermal amplification (LAMP) method to present a foundational technique of the diagnosis of Madurella spp. (M. mycetomatis, M. pseudomycetomatis, M. tropicana, and M. faha-lii), the common causative organisms of eumycetoma. Principal findings We successfully designed a primer pair targeting the rDNAs of three Madurella spp. exclud-ing M. fahalii, and detected up to 100 fg of genomic DNA extracted from isolates of M. myce-tomatis and 1 pg of M. pseudomycetomatis and M. tropicana, within one hour. Second, a primer pair specific to M. mycetomatis, the most common causative species, or M. fahalii, a drug-resistant species, was constructed, and the detection limit of both primer pairs was 1 pg. The designed primers accurately distinguished 16 strains of the genus Madurella from various fungal species known to cause mycetomas. Conclusion In summary, we established the first model of a LAMP detection method that rapidly and sensitively detects and identifies Madurella isolates for clinical diagnostics. Moreover, the combined designed primer sets could identify mycetoma-causing strains simultaneously.

Published
2023

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