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To reduce the water footprint in agriculture, the recent push toward precision irrigation management has initiated a sharp rise in photonics-based hydration sensing in plants in a non-contact, non-invasive manner. Here, this aspect of sensing was employed in the terahertz (THz) range for mapping liquid water in the plucked leaves of Bambusa vulgaris and Celtis sinensis. Two complementary techniques, broadband THz time-domain spectroscopic imaging and THz quantum cascade laser-based imaging, were utilized. The resulting hydration maps capture the spatial variations within the leaves as well as the hydration dynamics in various time scales. Although both techniques employed raster scanning to acquire the THz image, the results provide very distinct and different information. Terahertz time-domain spectroscopy provides rich spectral and phase information detailing the dehydration effects on the leaf structure, while THz quantum cascade laser-based laser feedback interferometry gives insight into the fast dynamic variation in dehydration patterns.

In this article, we revisit the concept of optical feedback regimes in diode lasers and explore each regime experimentally from a somewhat unconventional point of view by relating the feedback regimes to the laser bias current and its optical feedback level. The results enable setting the operating conditions of the diode laser in different applications requiring operation in different feedback regimes. We experimentally explored and theoretically supported this relationship from the standard Lang and Kobayashi rate equation model for a laser diode under optical feedback. All five regimes were explored for two major types of laser diodes: inplane lasers and vertical-cavity surface emitting lasers. For both lasers, we mapped the self-mixing strength vs. drive current and feedback level, observed the differences in the shape of the self-mixing fringes between the two laser architectures and a general simulation, and monitored other parameters of the lasers with changing optical feedback.

The phenomenon of self-pulsation (SP) in terahertz (THz) quantum cascade lasers (QCLs) due to optical feedback was reported recently. In this Letter, we propose a THz imaging modality using the SP phenomenon in a THz QCL. We explore the theoretical oscillation properties of the SP scheme and demonstrate its suitability to perform imaging experimentally. The SP imaging scheme operates in self-detection mode, eliminating the need for an external detector. Moreover, the scheme requires only a fixed current, meaning that one can avoid many of the pitfalls associated with high temperature operation of THz QCLs, including frequency chirp and mode hops caused by sweeping the laser current. This also means that one is free to locate the operating point at the maximum power, to produce the desired beam profile or for highest spectral purity, depending on the application. The SP imaging modality proposed in this work can be translated directly to high operating temperature THz QCLs.

Abstract The effects of optical feedback (OF) in lasers have been observed since the early days of laser development. While OF can result in undesirable and unpredictable operation in laser systems, it can also cause measurable perturbations to the operating parameters, which can be harnessed for metrological purposes. In this work we exploit this ‘self-mixing’ effect to infer the emission spectrum of a semiconductor laser using a laser-feedback interferometer, in which the terminal voltage of the laser is used to coherently sample the reinjected field. We demonstrate this approach using a terahertz frequency quantum cascade laser operating in both single- and multiple-longitudinal mode regimes, and are able to resolve spectral features not reliably resolved using traditional Fourier transform spectroscopy. We also investigate quantitatively the frequency perturbation of individual laser modes under OF, and find excellent agreement with predictions of the excess phase equation central to the theory of lasers under OF.

We propose a compact laser feedback interferometry imaging system for concurrent reflectance confocal microscopy and laser Doppler flowmetry. This system acquires both confocal reflectance and Doppler signals in a confocal architecture to image dynamic turbid media with higher contrast than a system operating in either modality and is coherent in nature. In a confocal optical configuration, reflectance confocal microscopy provides information about scattering from within a small volume centered around the focal point of the confocal system, and laser Doppler flowmetry provides information about the velocity of moving scatterers within the same volume. Raster scanning the sample enables the concurrent creation of two images, containing independent information, from a well-specified depth within the sample. Concurrent spatial mapping of these independent sensing modalities affords improvement in the capability of the imaging system by obtaining additional information from both morphological and functional features of the dynamic turbid medium at depths penetrable by near-infrared lasers. We realize the idea using a laser feedback interferometry imaging system scanning a microfluidic channel that contains a dynamic turbid medium. We show the effectiveness of this integrated imager quantitatively through the improvement of the signal-to-background ratio of a combined (multiplication) image.

Optical interrogation of suspicious skin lesions is standard care in the management of skin cancer worldwide. Morphological and functional markers of malignancy are often combined to improve expert human diagnostic power. We propose the evaluation of the combination of two independent optical biomarkers of skin tumours concurrently. The morphological modality of reflectance confocal microscopy (RCM) is combined with the functional modality of laser Doppler flowmetry, which is capable of quantifying tissue perfusion. To realize the idea, we propose laser feedback interferometry as an implementation of RCM, which is able to detect the Doppler signal in addition to the confocal reflectance signal. Based on the proposed technique, we study numerical models of skin tissue incorporating two optical biomarkers of malignancy: (i) abnormal red blood cell velocities and concentrations and (ii) anomalous optical properties manifested through tissue confocal reflectance, using Monte Carlo simulation. We also conduct a laboratory experiment on a microfluidic channel containing a dynamic turbid medium, to validate the efficacy of the technique. We quantify the performance of the technique by examining a signal to background ratio (SBR) in both the numerical and experimental models, and it is shown that both simulated and experimental SBRs improve consistently using this technique. This work indicates the feasibility of an optical instrument, which may have a role in enhanced imaging of skin malignancies.

We propose a self-consistent method for the analysis of granular materials at terahertz (THz) frequencies using a quantum cascade laser. The method is designed for signals acquired from a laser feedback interferometer, and applied to non-contact reflection-mode sensing. Our technique is demonstrated using three plastic explosives, achieving good agreement with reference measurements obtained by THz time-domain spectroscopy in transmission geometry. The technique described in this study is readily scalable: replacing a single laser with a small laser array, with individual lasers operating at different frequencies will enable unambiguous identification of select materials. This paves the way towards non-contact, reflection-mode analysis and identification of granular materials at THz frequencies using quantum cascade lasers.

Protein acetylation emerged as a key regulatory mechanism for many cellular processes. We used genetic analysis of Saccharornyces cerevisiae to identify Esa1 as a histone acetyltransferase required for autophagy. We further identified the autophagy signaling component Atg3 as a substrate for Esa1. Specifically, acetylation of K19 and K48 of Atg3 regulated autophagy by controlling Atg3 and Atg8 interaction and lipidation of AtgS. Starvation induced transient K19-K48 acetylation through spatial and temporal regulation of the localization of acetylase Esa1 and the deacetylase Rpd3 on pre-autophagosomal structures (PASs) and their interaction with Atg3. Attenuation of K19-K48 acetylation was associated with attenuation of autophagy. Increased K19-K48 acetylation after deletion of the deacetylase Rpd3 caused increased autophagy. Thus, protein acetylation contributes to control of autophagy. [ABSTRACT FROM AUTHOR]