Search

Your search keyword '"Yamamoto, Tadashi"' showing total 2,738 results
Start Over Author "Yamamoto, Tadashi"
2,738 results on '"Yamamoto, Tadashi"'

17. Development of a unit conversion tool for five quantitative anti‐spike assays and agreement analysis of three qualitative anti‐nucleocapsid assays for SARS‐CoV‐2.

Author

Ouoba, Serge, Sugiyama, Aya, Ko, Ko, Mirzaev, Ulugbek Khudayberdievich, Abe, Kanon, E, Bunthen, Phyo, Zayar, Khalilov, Kobiljon Khusniddin, Kurisu, Akemi, Akita, Tomoyuki, Takahashi, Kazuaki, Sasaki, Hiroshi, Yamamoto, Tadashi, and Tanaka, Junko

Abstract

Commercially available assays for measuring severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) anti‐spike (S) or anti‐nucleocapsid (N) antibodies differ in units, making results comparisons challenging. This study aimed to develop conversion equations between five quantitative anti‐S antibody tests and to assess the agreement over time between three qualitative anti‐N antibody tests. Blood samples from 24 216 vaccinated healthcare workers in Hiroshima Prefecture, Japan, were analyzed for anti‐S antibodies using five quantitative tests (Abbott, Fujirebio, Ortho, Sysmex, Roche) and for anti‐N antibodies using three qualitative tests (Abbott, Sysmex, Roche). Geometric mean regression was performed to establish equations for converting measured values between the five quantitative tests. Fleiss κ statistic was used to assess the agreement between the three qualitative tests. A strong correlation (Pearson's coefficient r > 0.9) was found for each pair of the five quantitative tests measuring anti‐S antibodies, enabling the development of equations to convert values between each pair. Using these equations, which are based on the original output unit of each test, values obtained from one test can be transformed to be equivalent to the corresponding values in another test. For the three tests for anti‐N antibodies, the agreement was substantial in the total sample (Fleiss' κ, 0.74) and moderate among those with self‐reported past coronavirus disease 2019 (COVID‐19) infection (Fleiss' κ, 0.39). The agreement decreased with time after infection. Reduced agreement between anti‐N antibodies tests over time suggests caution in comparing seroepidemiological studies of COVID‐19 exposure based on anti‐N antibodies measurement. The findings could help improve antibody measurement systems and inform public health decision‐makers. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

18. Silencing dentate newborn neurons alters excitatory/inhibitory balance and impairs behavioral inhibition and flexibility

Author

Li, Haowei, primary, Tamura, Risako, additional, Hayashi, Daiki, additional, Asai, Hirotaka, additional, Koga, Junya, additional, Ando, Shota, additional, Yokota, Sayumi, additional, Kaneko, Jun, additional, Sakurai, Keisuke, additional, Sumiyoshi, Akira, additional, Yamamoto, Tadashi, additional, Hikishima, Keigo, additional, Tanaka, Kazumasa Z., additional, McHugh, Thomas J., additional, and Hisatsune, Tatsuhiro, additional

Published
2024
Full Text
View/download PDF

24. Mass spectrometry-based proteomic analysis of proteins adsorbed by hexadecyl-immobilized cellulose bead column for the treatment of dialysis-related amyloidosis.

Author

Yamamoto, Suguru, Yamamoto, Keiko, Hirao, Yoshitoshi, Yamaguchi, Keiichi, Nakajima, Kichitaro, Sato, Mami, Kawachi, Miho, Domon, Mio, Goto, Kei, Omori, Kentaro, Iino, Noriaki, Shimada, Hisaki, Aoyagi, Ryuzi, Ei, Isei, Goto, Shin, Goto, Yuji, Gejyo, Fumitake, Yamamoto, Tadashi, and Narita, Ichiei

Subjects
MATRIX Gla protein, PROTEOMICS, PROTEIN analysis, BLOOD proteins, CHRONIC kidney failure, HEMOPERFUSION, CEREBRAL amyloid angiopathy, AFFINITY chromatography, CARPAL tunnel syndrome
Abstract

Dialysis-related amyloidosis (DRA) is a severe complication in end-stage kidney disease (ESKD) patients undergoing long-term dialysis treatment, characterized by the deposition of β2-microglobulin-related amyloids (Aβ2M amyloid). To inhibit DRA progression, hexadecyl-immobilized cellulose bead (HICB) columns are employed to adsorb circulating β2-microglobulin (β2M). However, it is possible that the HICB also adsorbs other molecules involved in amyloidogenesis. We enrolled 14 ESKD patients using HICB columns for DRA treatment; proteins were extracted from HICBs following treatment and identified using liquid chromatography–linked mass spectrometry. We measured the removal rate of these proteins and examined the effect of those molecules on Aβ2M amyloid fibril formation in vitro. We identified 200 proteins adsorbed by HICBs. Of these, 21 were also detected in the amyloid deposits in the carpal tunnels of patients with DRA. After passing through the HICB column and hemodialyzer, the serum levels of proteins such as β2M, lysozyme, angiogenin, complement factor D and matrix Gla protein were reduced. These proteins acted in the Aβ2M amyloid fibril formation. HICBs adsorbed diverse proteins in ESKD patients with DRA, including those detected in amyloid lesions. Direct hemoperfusion utilizing HICBs may play a role in acting Aβ2M amyloidogenesis by reducing the amyloid-related proteins. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

28. Genetic and chemical inhibition of IRF5 suppresses pre-existing mouse lupus-like disease

Author

Ban, Tatsuma, Kikuchi, Masako, Sato, Go R., Manabe, Akio, Tagata, Noriko, Harita, Kayo, Nishiyama, Akira, Nishimura, Kenichi, Yoshimi, Ryusuke, Kirino, Yohei, Yanai, Hideyuki, Matsumoto, Yoshiko, Suzuki, Shuichi, Hihara, Hiroe, Ito, Masashi, Tsukahara, Kappei, Yoshimatsu, Kentaro, Yamamoto, Tadashi, Taniguchi, Tadatsugu, Nakajima, Hideaki, Ito, Shuichi, and Tamura, Tomohiko

Published
2021
Full Text
View/download PDF

31. Psychiatric-disorder-related behavioral phenotypes and cortical hyperactivity in a mouse model of 3q29 deletion syndrome

Author

Baba, Masayuki, Yokoyama, Kazumasa, Seiriki, Kaoru, Naka, Yuichiro, Matsumura, Kensuke, Kondo, Momoka, Yamamoto, Kana, Hayashida, Misuzu, Kasai, Atsushi, Ago, Yukio, Nagayasu, Kazuki, Hayata-Takano, Atsuko, Takahashi, Akinori, Yamaguchi, Shun, Mori, Daisuke, Ozaki, Norio, Yamamoto, Tadashi, Takuma, Kazuhiro, Hashimoto, Ryota, Hashimoto, Hitoshi, and Nakazawa, Takanobu

Published
2019
Full Text
View/download PDF

32. NR2B Phosphorylation at Tyrosine 1472 Contributes to Brain Injury in a Rodent Model of Neonatal Hypoxia-Ischemia

Author

Knox, Renatta, Brennan-Minnella, Angela M, Lu, Fuxin, Yang, Diana, Nakazawa, Takanobu, Yamamoto, Tadashi, Swanson, Raymond A, Ferriero, Donna M, and Jiang, Xiangning

Subjects
Biomedical and Clinical Sciences, Neurosciences, Clinical Sciences, Perinatal Period - Conditions Originating in Perinatal Period, Stroke, Pediatric, Brain Disorders, Aetiology, 2.1 Biological and endogenous factors, 1.1 Normal biological development and functioning, Underpinning research, Neurological, Animals, Animals, Newborn, Blotting, Western, Disease Models, Animal, Fluorescent Antibody Technique, Gene Knock-In Techniques, Hypoxia-Ischemia, Brain, Immunoprecipitation, Mice, Mice, Inbred C57BL, Oxidative Stress, Phosphorylation, Receptors, N-Methyl-D-Aspartate, Tyrosine, Fyn tyrosine kinase, hypoxia-ischemia, brain, NR2B NMDA receptor, Cardiorespiratory Medicine and Haematology, Neurology & Neurosurgery, Clinical sciences, Allied health and rehabilitation science
Abstract

Background and purposeThe NR2B subunit of the N-methyl-d-aspartate (NMDA) receptor is phosphorylated by the Src family kinase Fyn in brain, with tyrosine (Y) 1472 as the major phosphorylation site. Although Y1472 phosphorylation is important for synaptic plasticity, it is unknown whether it is involved in NMDA receptor-mediated excitotoxicity in neonatal brain hypoxia-ischemia (HI). This study was designed to elucidate the specific role of Y1472 phosphorylation of NR2B in neonatal HI in vivo and in NMDA-mediated neuronal death in vitro.MethodsNeonatal mice with a knockin mutation of Y1472 to phenylalanine (YF-KI) and their wild-type littermates were subjected to HI using the Vannucci model. Brains were scored 5 days later for damage using cresyl violet and iron staining. Western blotting and immunoprecipitation were performed to determine NR2B tyrosine phosphorylation. Expression of NADPH oxidase subunits and superoxide production were measured in vivo. NMDA-induced calcium response, superoxide formation, and cell death were evaluated in primary cortical neurons.ResultsAfter neonatal HI, YF-KI mice have reduced expression of NADPH oxidase subunit gp91phox and p47phox and superoxide production, lower activity of proteases implicated in necrotic and apoptotic cell death, and less brain damage when compared with the wild-type mice. In vitro, YF-KI mutation diminishes superoxide generation in response to NMDA without effect on calcium accumulation and inhibits NMDA and glutamate-induced cell death.ConclusionsUpregulation of NR2B phosphorylation at Y1472 after neonatal HI is involved in superoxide-mediated oxidative stress and contributes to brain injury.

Published
2014

33. Personalized Preclinical Training in Dental Ergonomics and Endodontics in Undergraduate Dentistry Students (Pilot Study)

Author

Arias, Zulema, Haines, Stephanie, Yamamoto, Tadashi, Hatanaka, Kazu, Yamashiro, Keisuke, Sonoi, Norihiro, and Takashiba, Shogo

Subjects
endodontics, dental ergonomics, clinical skills improvement, undergraduate students, preclinical personalized education
Abstract

The curriculum at the Department of Pathophysiology in the Periodontal Sciences program at Okayama University includes normative preclinical training (NPT) using phantoms. NPT is given to the whole class of 5 th year students divided in groups of 8 students/instructor. In 2019, an innovative personalized preclinical training (PPT) pilot study was implemented for this group of students whereby two students, each with their own dental unit, were coached by one instructor. The main topics covered were dental ergonomics and endodontics. We aimed to evaluate the effectiveness of PPT in dental ergonomics and endodontics toward increasing the knowledge and future clinical skills of students who had already undergone NPT. A test on endodontics was taken before and after PPT. A questionnaire was completed to assess their perception of improvement regarding the above-mentioned topics. Test scores and questionnaire results both showed that the students’ level of knowledge and awareness of future clinical skills was significantly higher after PPT. This pilot study demonstrated that PPT increased the students’ knowledge and future clinical skills. As preclinical training forms the foundation for clinical practice, investment in future research regarding this personalized approach is likely to enhance students’ understanding and clinical performance.

Published
2023

Catalog

Books, media, physical & digital resources
See catalog results