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Thin layers and high-yield dolomite reservoirs were recently discovered in the Permian Guadeloupian Maokou Formation. The genetic mechanism of this reservoir is controversial because of its complex sedimentation and diagenesis in the Maokou Formation. Traditionally, the genesis has focused on sedimentation, karst, and fracture, whereas the influence of the Emeishan mantle plume activity (EMP) has been ignored. In this study, we enumerated petrographic (grouped into micritic bioclastic limestone, limy dolomite, grain dolomite, dolomite cement, calcite cement, and saddle dolomite) and geochemical data (δ13C, δ18O, REE, and 87Sr/86Sr) from a microscopic perspective to support the impact of EMP on reservoirs. We conclude that EMP activity altered the sedimentary environment and induced a complex diagenesis. The paleogeomorphic reconstruction data indicate that the EMP caused an uplift zone in the NE–SW direction, depositing advantageous high-energy beach facies. In terms of diagenesis, the abnormally high 87Sr/86Sr ratios and REE with positive Eu anomalies suggest that dolomitization was influenced by both seawater and hydrothermal fluids. Based on the above evidence, we established a reservoir genetic model for the Maokou Formation related to the intensity of the EMP. This study provides a new perspective on the mantle plume activity for the study of carbonate reservoir genesis. [ABSTRACT FROM AUTHOR]