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Background: As the fight against the COVID-19 epidemic continues, medical workers may have allostatic load. Objective: During the reopening of society, medical and nonmedical workers were compared in terms of allostatic load. Methods: An online study was performed; 3,590 Chinese subjects were analyzed. Socio-demographic variables, allostatic load, stress, abnormal illness behavior, global well-being, mental status, and social support were assessed. Results: There was no difference in allostatic load in medical workers compared to nonmedical workers (15.8 vs. 17.8%; p = 0.22). Multivariate conditional logistic regression revealed that anxiety (OR = 1.24; 95% CI 1.18–1.31; p < 0.01), depression (OR = 1.23; 95% CI 1.17–1.29; p < 0.01), somatization (OR = 1.20; 95% CI 1.14–1.25; p < 0.01), hostility (OR = 1.24; 95% CI 1.18–1.30; p < 0.01), and abnormal illness behavior (OR = 1.49; 95% CI 1.34–1.66; p < 0.01) were positively associated with allostatic load, while objective support (OR = 0.84; 95% CI 0.78–0.89; p < 0.01), subjective support (OR = 0.84; 95% CI 0.80–0.88; p < 0.01), utilization of support (OR = 0.80; 95% CI 0.72–0.88; p < 0.01), social support (OR = 0.90; 95% CI 0.87–0.93; p < 0.01), and global well-being (OR = 0.30; 95% CI 0.22–0.41; p < 0.01) were negatively associated. Conclusions: In the post-COVID-19 epidemic time, medical and nonmedical workers had similar allostatic load. Psychological distress and abnormal illness behavior were risk factors for it, while social support could relieve it.

This study aimed to analyse the efficacy of haploidentical donor (HID) haematopoietic stem cell transplantation as a first-line treatment for severe aplastic anaemia (SAA) with high-risk factors (infection or very severe aplastic anaemia,VSAA) in patients who lack an HLA-matched sibling donor (MSD). The patients with infection were treated with anti-infection therapy, and allogeneic haematopoietic stem cell transplantation (HSCT) was carried out after the infection being effectively controlled was in accordance with the stable infection (SI) standard. A total of 44 SAA patients receiving MSD transplantation (n = 19) and HID transplantation (n = 25) were included in this study. There was no significant difference in neutrophil engraftment between the two groups [MSD vs. HID, 19 (11–38) vs. 22 (15–47).P = 0.241], and the difference in platelet engraftment was statistically significant [MSD vs. HID, 11(7–33) vs. 20 (12–69), P = 0.034]. The HID group exhibited a higher incidence of grade II–IV acute graft-versus-host disease (aGVHD) (HID vs. MSD, 48.0% vs10.5%, P = 0.034)and a higher incidence of chronic GVHD (cGVHD) than the MSD group (64.0% vs. 21.1%, P = 0.026). There was no significant difference between overall survival (OS) following HID and MSD transplantation (84.0% vs. 89.5%, P = 0.664) and failure-free survival (FFS)(80.0% vs. 84.2%, P = 0.965). The interval from diagnosis to transplantation (> 50d) and ECOG (> 2) were independent factors associated with OS and FFS. HID HSCT may be an effective and safe option for SAA patients with high-risk factors who lack an MSD.

Objective To observe the effects of scalp penetration acupuncture on the expression of nerve growth factor (NGF) in brain tissue around hematoma of intracerebral hemorrhage rats. Methods To- tally 120 healthy male Wistar rats were divided into the sham-operation group, the model group, the acupunc- ture group by random digit table, 40 in each group. Rats in each group were further randomly divided into 4 time points, i.e., 6 h, 24 h, day 3, day 7. The intracerebral hemorrhage rat model was prepared by modified autologous arterial blood method, intervened by electroacupuncture (EA) at Baihui (DU20) through Taiyang (EX- HN5). Neuroethological assessment was conducted by Longa score. The expression of NGF positive cells in brain tissue around hematoma was detected using immunohistochemical method. The amount of NGF mRNA expression was measured by q-PCR. Results Compared with the sham-operation group, neurobehavioral scores increased, NGF positive cells increased, NGF mRNA expression increased in the model group at each time point, with statistical difference shown (P0.05). Compared with the model group, 6 h neurobehavioral scores,NGF positive cells, mRNA expression of NGF did not change significantly in the acupuncture group, with no statistical difference shown (P0. 05). Neuroethological scores decreased, NGF positive cells increased, mRNA expression of NGF was up-regulated in the acupuncture group at 24 h, day 3 and 7, respec- tively, with statistical difference shown (P0. 05). Conclusion scalp penetration acupuncture could up-regulate gene and protein expressions of NGF, and promote recovery of nerve function, showing favorable neuro-protective effects.

Three new indole diterpenes, penicilindoles A–C (1–3), were isolated from the mangrove-derived fungus Eupenicillium sp. HJ002. Their planar structures and absolute configurations were determined by interpretation of NMR spectroscopic data, HR-ESIMS, and X-ray diffraction analysis using Cu Kα radiation. The cytotoxic and antibacterial activities were evaluated in vitro; penicilindole A (1) showed cytotoxic activity against human A549 and HepG2 cell lines with IC50 values of 5.5 and 1.5 μM, respectively.

Chemical investigation on the endophytic fungus Penicillium sp. B21 from the leaf of Bruguiera sexangula var. rhynchopetala led to the isolation of a new compound, penicitrinone acetate (1), the structure of which was elucidated by spectroscopic methods, including 2D NMR techniques.

Three new dihydroisocoumarin penicimarins G–I (1–3), together with one known dihydroisocoumarin (4) and three known meroterpenoids (5–7), were obtained from a fungus Penicillium citrinum isolated from the mangrove Bruguiera sexangula var. rhynchopetala collected in the South China Sea. Their structures were elucidated by the detailed analysis of spectroscopic data. The absolute configuration of 1 was determined by the X-ray diffraction analysis using Cu Kα radiation. The absolute configurations of 2 and 3 were determined by comparison of their circular dichroism (CD) spectra with the literature. All compounds were evaluated for their antibacterial activities and cytotoxic activities.

Silica-zirconia mixed oxide (SiO 2 -ZrO 2 ) was synthesized by sol-gel method; with SiO 2 -ZrO 2 as the support, CuCl 2 /SiO 2 -ZrO 2 catalyst was then prepared by ion-exchange and used in the liquid oxidative carbonylation of methanol to dimethyl carbonate (DMC). The results showed that the introduction of zirconium species into the amorphous silica leads to the formation of Si-O-Zr bonds and Bronsted acid sites. When the CuCl 2 /SiO 2 -ZrO 2 catalyst is calcined at 300–550°C in an inert atmosphere, CuCl 2 is dispersed and bounded on the surface of SiO 2 -ZrO 2 support; CuCl 2 can be auto-reduced to CuCl, which is then ion-exchanged with the surface Bronsted acid sites of the SiO 2 -ZrO 2 support to form Cu + species. As a result, most of copper remains to be highly dispersed on the surface of SiO 2 -ZrO 2 and only a small amount of chlorine is left over. The CuCl 2 /SiO 2 -ZrO 2 catalyst calcined at 450°C exhibits excellent activity in the oxidative carbonylation of methanol; under the conditions of 140°C, 2.4 MPa and a CO/O 2 ratio of 2, the conversion of methanol and the selectivity to DMC reach 10.0% and 79.4%, respectively.

To explore the effect of bortezomib (BOR) on the drug sensitivity of imatinib-resistant chronic myeloid leukemia cell line K562/G01 cell and its mechanism.MTT assay was used to detect the inhibition effect of cell growth, flow cytometry to cell cycle, and real time-PCR to the expression of COX-2 and mdr1 mRNA.Combination of 10 and 20 nmol/L BOR with imatinib could significantly enhance the sensitivity of K562/G01 to imatinib, the reverse factor was 1.83 and 2.72-fold respectively. Cell cycle arrested at G(2)/M phase could be observed by flow cytometry on BOR treatment. The over-expression of COX-2 and mdr1 could be down-regulated by BOR.BOR can enhance the imatinib sensitivity of imatinib resistant K562/G01 cell. The mechanism may be related to cell cycle phase arrested at G2/M and down-regulation of COX-2 and mdr1 expression.

To summarize the clinical features of laryngeal neuroendocrine carcinoma (LNEC).The clinical data of all LNEC patients hospitalized during the period of June 1988 - December 2010 were analyzed retrospectively.Among all LNEC 12 patients as confirmed by pathological examination, there were typical carcinoid (n = 1), atypical carcinoid (n = 4) (2 with cervical metastasis, 1 with paraneoplastic syndrome), small cell neuroendocrine carcinoma (n = 7) (1 with adenocarcinoma). All patients with typical and atypical carcinoid underwent surgery. And postoperative radiotherapy was offered for those diagnosed with advanced stage or cervical metastasis. They were followed up for 1 - 12 years without local recurrence. All patients with laryngeal small cell neuroendocrine carcinoma received chemotherapy and radiotherapy. And the follow-up period was 0.5 - 6 years. One died of pulmonary metastasis within half a year while another died of liver metastasis within 3 years.It is important to classify the LNEC patients correctly since their clinical course, treatment and prognosis varies greatly with the diagnosis. For typical and atypical carcinoid of larynx, partial or total laryngectomy and neck dissection may be performed depending on the site and extent of primary tumor. However, for laryngeal small cell neuroendocrine carcinoma, the mainstay of treatment is made up of chemotherapy and radiotherapy. And the prognosis of this type remains very poor.

We investigated the short-term and medium-term results in patients with pulmonary arterial hypertension (PAH) associated with atrial septal defect (ASD) undergoing transcatheter closure. Fifteen patients with severe PAH associated with ASD who underwent successful occluder implantation from 2007 to 2010 were included. Clinical, echocardiographic, and hemodynamic data were reviewed. Severe PAH was defined as pulmonary arterial systolic pressure measured by catheterization was ≥60 mmHg and pulmonary vascular resistance (PVR) ≥6 Wood Units (WU). Compared with baseline, the 6-minwalking distance significantly increased by 29.7 ± 26.3 m (P < 0.001) at 3 months (short-term) and 65.4 ± 63.6 m (P < 0.001) at 23.4 ± 9.7 months (medium-term), World Health Organization function class considerably improved after postclosure short-term and medium-term. Repeat cardiac catheterization (n = 7) showed that mean pulmonary arterial pressure decreased from 51.6 ± 9.4 mmHg at baseline to 21.0 ± 3.8 mmHg (P < 0.001) at follow-up of 12 months. The PVR decreased by 5.6 ± 1.1 WU (P < 0.001). Through carefully selected patients with severe PAH associated with ASD, transcatheter closure can be safely performed with a promising short-term and medium-term outcome. Trial occlusion is an effective way for deciding the reversibility of severe PAH in ASD patients. The role of aerosolized iloprost for pulmonary vasoreactivity testing in patients with severe PAH secondary to ASD requires further investigation.

This study was aimed to investigate the relationship between CD4(+)CD25(+) regulatory T cells, IL-2, TGF-beta and acute graft-versus host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). The percentage of peripheral blood CD4(+)CD25(+) Treg cells in CD4(+) T cells of 13 patients with hematological malignancies after allo-HSCT were detected by flow cytometry; serum levels IL-2 and TGF-beta in these patients were measured by ELISA. The results indicated that all the patients achieved engraftment. 5 patients developed aGVHD of grade I-II, 4 patients developed aGVHD of grade III-IV. The percentage of peripheral blood CD4(+)CD25(+) Treg cells out of CD4(+) T cells in patients without aGVHD was higher than that in patients with aGVHD (p0.05); the serum level of IL-2 in patients without aGVHD was lower than that of patients with aGVHD (p0.05); the serum level of TGF-beta in patients without aGVHD was higher than that of patients with aGVHD (p0.05). It is concluded that CD4(+)CD25(+) Treg cell level and the serum level of IL-2 and TGF-beta all are related to incidence and severity of aGVHD. These factors may be used as indicators for early evaluating and monitoring aGVHD after allo-HSCT.