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553 results on '"Yang, Huifang"'

8. A Search of Only Four Key Databases Would Identify Most Randomized Controlled Trials of Acupuncture: A Meta-Epidemiological Study

Author

Guo, Qiong, Cheng, Yifan, Zhang, Chenyang, Yang, Huifang, Chen, Xia, Wang, Xinyi, Yang, Liu, Feng, Kun, Long, Youlin, Shao, Zilun, Wang, Yutian, Lin, Yifei, Liao, Ga, Huang, Jin, and Du, Liang

Abstract

Little research has been conducted to assess which specific databases should be searched when performing a systematic review (SR) on acupuncture. The current study aimed to identify key databases and the optimal database combination to retrieve randomized controlled trials (RCTs) on acupuncture for inclusion in SRs. A systematic search for SRs in the field of acupuncture was conducted in order to identify target databases and RCTs were extracted from the SRs that had searched all target databases. The proportions of SRs that had achieved 100%, 95%, or 90% recall of RCTs and the total recall of RCTs in various combinations of target databases were calculated. Sensitivity analysis was performed on those SRs that included 10 or more RCTs. CNKI, WanFang, VIP, PubMed, CENTRAL and Embase were regarded as target databases. A total of 4349 acupuncture RCTs were extracted from 286 SRs. Searching all six target databases retrieved 99.3% of all RCTs while 99.1% were recalled by searching the combination of CNKI, WanFang, PubMed and CENTRAL. There were no significant differences on total recall of RCTs (p = 0.549) or in the proportion of SRs with 90% recall of RCTs (97.2% vs. 97.6%; p = 0.794) between searching the above four and the full six target databases. Most results were similar in the sensitivity analysis. The combined retrieval power of CNKI, WanFang, PubMed and CENTRAL was considered an efficient choice to retrieve acupuncture RCTs included in SRs.

Published
2022
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25. Decouple U-Net: A Method for the Segmentation and Counting of Macrophages in Whole Slide Imaging

Author

Chen, Zining, Yang, Huifang, Gao, Mengqi, Hu, Xiao, Li, Yunpeng, Wang, Lin, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Yu, Shiqi, editor, Zhang, Zhaoxiang, editor, Yuen, Pong C., editor, Han, Junwei, editor, Tan, Tieniu, editor, Guo, Yike, editor, Lai, Jianhuang, editor, and Zhang, Jianguo, editor

Published
2022
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31. Benzo[k] fluoranthene induces male germ cell damage

Author

WANG Dandan, WANG Furong, YANG Xiaoyan, ZHOU Niya, YANG Huifang, and CAO Jia

Subjects
benzo[k] fluoranthene, gc-2spd cells, oxidative damage, ahr-cyp1a1 signaling pathway, bioinformatics analysis, Medicine (General), R5-920
Abstract

Objective To establish a damage model of mouse spermatocyte GC-2spd cells induced by benzo[k] fluoranthene (BkF), a representative compound of high-molecular weight polycyclic aromatic hydrocarbons, so as to investigate the effect and mechanism of BkF on GC-2 cells. Methods Related genes of male reproductive diseases associated with BkF were screened in Comparative Toxicogenomics Database (CTD), and functional and pathway enrichment analysis is conducted subsequently to explore the possible pathway mechanism. Moreover, GC-2 cells were treated with BkF at a final concentration of 0, 10, 20, 40 or 80 μmol/L for 72 h, and then the cell cycle, oxidative stress and DNA damage effect were detected. Western blotting was used to determine the protein levels of the key genes as well as the core molecules of related pathways. Results The key genes CYP1A1 and CYP17A1 associated with BkF-related reproductive system diseases were screened out from the CTD database. Gene Ontology (GO) enrichment analysis found that they were involved in male reproductive system and cell cycle arrest, obviously enriched in biological processes such as oxidative stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that these genes were significantly enriched in cytochrome P450 pathway (P < 0.01). After GC-2 cells were exposed to BkF for 72 h, the proliferation was significantly decreased in a dose-dependent manner, as compared with the control group. When the exposure dose was higher than 40 μmol/L, the cell cycle arrest was obvious (P < 0.05); Both the intracellular reactive oxygen species (ROS) level (P < 0.05) and the protein level of DNA damage marker γ-H2AX (P < 0.01) were remarkably increased in a dose-dependent manner. In addition, the protein levels of CYP1A1 and CYP17A1 were greatly higher in the exposure group than the control group (P < 0.05), while treatment of AhR inhibitor CH223191 resulted in notably down-regulated the expression of the 2 proteins (P < 0.05). Conclusion BkF induces the activation of AhR receptors, then causes elevated expression of downstream genes CYP1A1 and CYP17A1 as well as increased intracellular ROS, and finally leads to oxidative stress and genetic damage in GC-2 cells.

Published
2022
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