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27. Additional file 7 of Proteomic and metabolic profile analysis of low-temperature storage responses in Ipomoea batata Lam. tuberous roots

Author

Cui, Peng, Yongxin Li, Chenke Cui, Yanrong Huo, Guoquan Lu, and Yang, Huqing

Abstract

Additional file 7: Table S1. Information of sweetpotato materials. Table S2. Information of differentially expressed proteins. Table S3. Number of differentially expressed metabolisms. Figure S1. SDS-PAGE of total proteins extracted from root tuber of Ipomoea batatas L.. (30 μg total proteins each lane). Figure S2. Distribution of proteins according to molecular weights.

Published
2020
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28. Effect of Chitosan Coatings Enriched with Lauroyl Arginate Ethyl and Montmorillonite on Microbial Growth and Quality Maintenance of Minimally Processed Table Grapes (Vitis vinifera L. Kyoho) During Cold Storage

Author

Huiyun Chen, Zhidong Sun, Yang Huqing, and Jiasi Hao

Subjects
food.ingredient, Pectin, Aerobic bacteria, Chemistry, Process Chemistry and Technology, fungi, food and beverages, Cold storage, Titratable acid, 04 agricultural and veterinary sciences, Ascorbic acid, Shelf life, 040401 food science, Polyphenol oxidase, Industrial and Manufacturing Engineering, 0404 agricultural biotechnology, food, hemic and lymphatic diseases, Browning, Food science, Safety, Risk, Reliability and Quality, Food Science
Abstract

The effects of lauroyl arginate ethyl (LAE), chitosan (CTS), and CTS-containing montmorillonite and LAE coatings (CML) on the quality maintenance and shelf life of minimally processed grapes stored at 4 °C for 20 days were investigated. Compared with the control, LAE, and pure CTS coatings, the CML coatings effectively decreased grape weight loss, respiration, and decay incidence as well as microorganism counts of total aerobic bacteria, yeasts, molds, and lactic acid bacteria in grapes. The CML coatings also showed better effects on delaying the tissue softening and browning due to the decreased activities of polygalacturonase, pectin methyl esterase, and polyphenol oxidase. CML coatings did not significantly change the content of soluble solids and the titratable acidity or the pH value of grapes, compared with other samples. However, CML coatings ensured higher content of ascorbic acid and sensory quality in grapes. The results confirmed that CML coatings improve microbiological safety and preserve nutritional and sensory quality of minimally processed grapes.

Published
2018

33. Metabolomic and Transcriptomic Analyses of Quality Deterioration in Fusarium solani-Infected Sweet Potato (Ipomoea batatas (L.)Lam cv Xinxiang) Storage Roots

Author

Li, Yongxin, Yin, Yuting, Golding, John B., Geng, Shuxian, Chen, Gang, and Yang, Huqing

Abstract

Fusarium solani-induced quality deterioration in stored sweet potato is poorly characterized and understood. This study examined the effects of F. solaniinfection in Xinxiang sweet potato roots during storage. The results showed that while there were no external symptoms following F. solaniinfection, upon cutting the roots, the cut surface of the infected root rapidly turned black, whereas the untreated control roots remained unaffected. The metabolites and transcriptive differences between F. solani-infected and control sweet potato roots were investigated with high-performance liquid chromatography, metabolomic analysis, and an Illumina Novaseq platform. The results showed that levels of the toxic ipomeamarone accumulated as high as 2.36 mg/kg DW in tissue after F. solaniinoculation and 6 days storage at 28 °C, where the control tissue sample did not accumulate any ipomeamarone. Metabolomic analysis showed that isochlorogenic acid and l-tyrosine significantly increased in the infected tissue and associated with the darkening cut surface of the infected sweet potato. In transcriptomic analysis, a total of 13, 14, and 6 key genes in ipomeamarone, isochlorogenic acid, and l-tyrosine biosynthesis pathways, respectively, were identified. A conceptual model elucidating the physiological and molecular mechanism of F. solani-induced quality deterioration in sweet potato is proposed.

Published
2022
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38. Effect of hypobaric storage on flesh lignification, active oxygen metabolism and related enzyme activities in bamboo shoots

Author

Zhidong Sun, Fenghua Wu, Huiyun Chen, Yang Huqing, Lingju Zhang, and Jiangang Ling

Subjects
Antioxidant, biology, Chemistry, medicine.medical_treatment, Flesh, food and beverages, APX, Malondialdehyde, Superoxide dismutase, chemistry.chemical_compound, Horticulture, Catalase, Botany, Shoot, biology.protein, medicine, Food Science, Peroxidase
Abstract

Freshly harvested bamboo (Phyllostachys violascens) shoots were stored for 35 days under various hypobaric conditions (101, 75, 50 and 25 kPa) at 2 ± 1 °C to investigate the effects of hypobaric storage on flesh lignifications. It was found that hypobaric storage at 50 kPa was most effective in preventing the increase in shoots firmness and allied accumulations of lignin and cellulose. Furthermore, the hypobaric storage at 50 kPa inhibited the ethylene production, reduced the rate of O 2 · − production and the accumulation of malondialdehyde (MDA) and H 2 O 2 , and maintained significantly higher activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX), but restrained the activities of phenylalanine ammonia-lyase (PAL) and peroxidase (POD). These data indicate that the delay in flesh lignification of bamboo shoots by hypobaric storage was due to maintenance of higher antioxidant enzymes activities and reduced ethylene production, leading to less ROS accumulation and better cell-membrane integrity.

Published
2013

40. Conversion of Bamboo Shoot Shell Biomass Into C6-Sugars by [C4mim]HSO4 Catalyzed Hydrolysis

Author

Zhang Youzuo, Yu Xiaojie, Zhou Cunshan, Yang Huqing, Lin Lin, and Wang Yunxiang

Subjects
Chemical kinetics, chemistry.chemical_compound, Hydrolysis, chemistry, Inorganic chemistry, Kinetics, Levulinic acid, Biomass, Fractionation, Bamboo shoot, Catalysis
Abstract

The C6-sugars from bamboo shoot shell biomass (BSSB) fraction are expected to be converted into 5-hydroxymethyl-2-furfural (HMF), and subsequently to levulinic acid (LA). A kinetic study on one of the key steps of biomass to C6-sugars has been investigated. The experiments were performed in temperature (110-150°C), using [C4mim]HSO4 as the catalyzt (0.1-1.0 mol L-1) and a initial intake of 3% BSSB. A kinetic model of the reaction was developed for the kinetics of C6-sugars using a pseudohomogeneous, first-order kinetic model, which provided a satisfactory interpretation for the experimental results. The kinetic parameters for the model provided useful information for understanding the conversion mechanism of biomass into C6-sugars, HMF and LA.

Published
2011

43. Combined Application of Carboxymethyl Chitosan Coating and Brassinolide Maintains the Postharvest Quality and Shelf Life of Green Asparagus.

Author

Wu, Lingyan and Yang, Huqing

Subjects
*ASPARAGUS, *CARBOXYMETHYL compounds, *CHITOSAN, *BRASSINOLIDE, *SHELF-life dating of food
Abstract

The combined effects of carboxymethyl chitosan ( CMCH) coating and brassinolide ( BR) on the sensory quality and physicochemical characteristics of green asparagus ( A sparagus officinalis L.) stored at 4 ± 1C and 90-95% relative humidity for 24 days were investigated. The combined treatment ( CMCH + BR) inhibited respiration rate and water loss, maintained higher concentrations of ascorbic acid, total phenolics and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging activity, and restrained the increase of malondialdehyde and electrolyte leakage of asparagus spears during storage. The activities of superoxide dismutase, ascorbate peroxidase and catalase were enhanced by CMCH + BR, while the activity of phenylalanine ammonia lyase was significantly inhibited, which resulted in lower lignin content and tender texture. Sensory evaluation also confirmed that the efficacy of CMCH + BR treatment was better than that of CMCH coating or BR treatment alone. Our study suggests that CMCH + BR coating has the potential to maintain the quality of asparagus spears and extend its postharvest life. Practical Applications This paper investigates the effect of carboxymethyl chitosan combined with brassinolide on the storage quality of green asparagus. The results presented demonstrate that the effect of the combined treatment on the sensory quality and physicochemical characteristics should be considered by processors as a preservation technique. [ABSTRACT FROM AUTHOR]

Published
2016
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49. 6-Benzylaminopurine alleviates chilling injury of postharvest cucumber fruit through modulating antioxidant system and energy status.

Author

Chen, Bingxia and Yang, Huqing

Abstract

BACKGROUND 6-Benzylaminopurine (6- BA) has a close relationship with the stress resistance of plants. Little research has been carried out on the effects of 6- BA on the cold resistance of postharvest fruits and vegetables. Therefore the aim of the present study was to evaluate the effects of 6- BA on chilling injury ( CI), antioxidant system and energy status in cucumber during storage. RESULTS The results showed that 6- BA at 50 mmol L−1 was most effective to restrain CI in cucumber fruit. Fruits treated with 50 mmol L−1 6- BA maintained higher levels of chlorophyll, ascorbic acid, total phenolics and total antioxidant capacity. Furthermore, this treatment reduced the increases in membrane permeability and lipid peroxidation, delayed the increases in both rate of [ABSTRACT FROM AUTHOR]

Published
2013
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