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In the current study, data collected from Wave 6 of the Millennium Cohort Study (n = 11,872), a nationally representative sample survey of youth aged 14 years in the UK, are used to examine the prevalence of loneliness among this age-group, investigate the feelings associated with the experience of loneliness among youth, explore the risk factors for loneliness among young people, and learn how they coped with loneliness. Given recent findings that youth are vulnerable to loneliness, the study assesses the prevalence of loneliness among adolescents across some important sociodemographic characteristics, such as nation of residence, gender, and ethnicity. We also identify the kinds of social experiences that accompany loneliness during adolescence, exploring friendship, relations with parents, social support, and bullying. Our key finding is that, in addition to the absence of desired social relationships, which has been typically identified as the ultimate source of loneliness, the presence of undesirable and even harmful social relationships is a major source of loneliness. This study uniquely brings together psychological and sociological perspectives to understand the experience of youth loneliness.

Coal mining under the Quaternary thick loose layer affects key strata breakage, Bed-separations development, ground subsidence, and other studies. This paper presents a method for solving the deflection of a large-deflection inclined thin plate under a thick loose-layer cover with additional lateral loads and midplane forces. The methods presented are based on the principle of large-deflection of thin-plate, energy method, and fracture mechanics theory. The 7225 work face in Anhui Province, China, was studied. Combined with the large-deflection inclined thin plate model, the initial breakage distance within the main roof plate was calculated to be 33 m with the initial breakage angle of 61.2°, and the period breakage distance was calculated to be 21 m with the period breakage angle of 55.4°. The distribution range of "Vertical Three Zones" from 7225 working face to the ground, including the height of the caved zone is 38.07 m, the height of the fractured zone is 41.13 m, and the height of the curved zone with the thick loose layer removed is 187.56 m. During the dynamic development of the principal key strata (PKS), the deflection value develops from 0 mm to 2714 mm with 7225 working face mining, and the maximum value of the spatial volume is 56,485 m3, which is verified by Three-dimensional Discrete Element Code (3DEC) numerical simulation. The dynamic development of Bed-separation within the overlying strata, with a maximum development height of 545.2 mm and a maximum volume of 11,228.1 m3 of the Bed-separation cavity. The dynamic development of the Bed-separation height and the cavity under different mining length and width conditions of the working face are also discussed. The large-deflection inclined thin plate model proposed in this paper effectively explores the dynamic deflection and fragmentation law of the overlying strata induced by the inclined working face of Longwall mining and provides a theoretical basis and computational model for quantitatively evaluating the dynamic development of the Bed-separation cavity. [ABSTRACT FROM AUTHOR]

While temperature has been shown to affect the survival and growth of bacteria and their phage parasites, it is unclear if trade-offs between phage resistance and other bacterial traits depend on the temperature. Here, we experimentally compared the evolution of phage resistance–virulence trade-offs and underlying molecular mechanisms in phytopathogenic Ralstonia solanacearum bacterium at 25 °C and 35 °C temperature environments. We found that while phages reduced R. solanacearum densities relatively more at 25 °C, no difference in the final level of phage resistance was observed between temperature treatments. Instead, small colony variants (SCVs) with increased growth rate and mutations in the quorum-sensing (QS) signaling receptor gene, phcS , evolved in both temperature treatments. Interestingly, SCVs were also phage-resistant and reached higher frequencies in the presence of phages. Evolving phage resistance was costly, resulting in reduced carrying capacity, biofilm formation, and virulence in planta, possibly due to loss of QS-mediated expression of key virulence genes. We also observed mucoid phage-resistant colonies that showed loss of virulence and reduced twitching motility likely due to parallel mutations in prepilin peptidase gene, pilD. Moreover, phage-resistant SCVs from 35 °C-phage treatment had parallel mutations in type II secretion system (T2SS) genes (gspE and gspF). Adsorption assays confirmed the role of pilD as a phage receptor, while no loss of adsorption was found with phcS or T2SS mutants, indicative of other downstream phage resistance mechanisms. Additional transcriptomic analysis revealed upregulation of CBASS and type I restriction-modification phage defense systems in response to phage exposure, which coincided with reduced expression of motility and virulence-associated genes, including pilD and type II and III secretion systems. Together, these results suggest that while phage resistance–virulence trade-offs are not affected by the growth temperature, they could be mediated through both pre- and postinfection phage resistance mechanisms. [ABSTRACT FROM AUTHOR]

Existing dynamic single-point subsidence prediction methods of mining area earth-surface are mainly based on traditional leveling measurement data, which have single monitoring mode, high cost and easily destroyed observation points, and cannot guarantee real-time of earth-surface deformation information. Moreover, application of grey model for earth-surface subsidence prediction is only for a single model and its applicability is not analyzed according to characteristics of the model itself. Taking 7221 working face of Yuandian No.2 Mine as an experimental zone, earth-surface subsidence value is measured by differential interferometry synthetic aperture radar technology, and then GM(1,1) and grey Verhulst model that describe the relationship between subsidence and time are built separately for predicting earth-surface subsidence value, so as to realize the integration of earth-surface subsidence monitoring and dynamic prediction of mining area. Through comparing and analyzing fitting and prediction results of earth-surface subsidence value between GM(1,1) and grey Verhulst model, the applicability of the two grey models in earth-surface subsidence prediction of mining area is obtained: GM(1,1) should be used for short-term prediction from beginning of mining subsidence to early active stage if subsidence value curve of single point is approximately unimodal; when earth-surface subsidence enters into decline stage and subsidence value curve of single point is in flat-bottom saturation, gray Verhulst model should be used for medium and long-term prediction.