Search

Additional file 1: Figure S1. Genome map of B. velezensis PG12. Each circle has a different genome information, and circles from outside to inside: (1) scale marks (unit: Mb), (2, 3) protein-coding genes on the forward and reverse strands, respectively (color-coded by the functional categories), (4, 5) rRNA (blue) and tRNA (red) on the forward and reverse strands, respectively, (6) GC content (positive: red; negative: blue), and (7) GC skew (above average: aquamarine; below average: orange). Figure S2. Genome comparisons of FZB42 with other three B. velezensis strains. The inner cycle represents the complete genome sequence of FZB42, and the shade of each color shows the similarities between the strains (CAU B946, UCMB5036 or PG12) and FZB42. Figure S3. Venn diagram showing shared orthologous protein clusters in the genomes of PG12 and its closely related B. velezensis strains CAU B946, FZB42 and UCMB5036. Each strain is represented by an oval. The number of orthologous protein-coding genes shared by all strains is in the center. Overlapping regions show the number of coding sequences conserved only within the specified genomes. Numbers in the non-overlapping portions of each oval show the number of CDS unique to each strain. The total number of protein-coding genes within each genome is listed below the strain name. Figure S4. Phylogenetic tree showing the relationship of the B. amyloliquefaciens group strains. The tree is based on the concatenated alignments of 860 single core genomes. Paenibacillus polymyxa M1 was used as an outgroup. Figure S5. Pan- and core-genome plots of the B. amyloliquefaciens group strains. a B. velezensis; b B. siamensis; c B. amyloliquefaciens. The lines in yellow and purple represent the pan- and core-genomes, respectively. Figure S6. Organization of lipopeptide gene clusters in draft genome sequence of the B. amyloliquefaciens group strains. Different genes are marked with different colors. Figure S7. Comparative analysis of G + C contents between genomes and the fengycin gene clusters (fenB, fenC and fenD) in the B. amyloliquefaciens group strains. Figure S8. Neighbor-joining phylogenetic tree constructed based on the protein sequences of fenB. Figure S9. Neighbor-joining phylogenetic tree constructed based on the protein sequence of fenC. Figure S10. Neighbor-joining phylogenetic tree constructed based on the protein sequence of fenD. Figure S11. The antifungal activity of PG12 and the ���fenA mutant. A mycelium plug of fungal pathogen was inoculated onto the center of a PDA plate, and then 2 ��L of PG12 and ���fenA cultures in LB medium were added at a distance of 2.5 cm from the rim of the fungal plug. Clear inhibition zones were observed after incubation for 5���7 days.