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48 results on '"Yang, Shun-Kai"'

3. Cryo electron tomography with volta phase plate reveals novel structural foundations of the 96-nm axonemal repeat in the pathogen Trypanosoma brucei.

Author

Imhof, Simon, Zhang, Jiayan, Wang, Hui, Bui, Khanh Huy, Nguyen, Hoangkim, Atanasov, Ivo, Hui, Wong H, Yang, Shun Kai, Zhou, Z Hong, and Hill, Kent L

Subjects
Trypanosoma brucei brucei, Protozoan Proteins, Imaging, Three-Dimensional, Cryoelectron Microscopy, Protein Binding, Axoneme, Electron Microscope Tomography, axoneme, cell biology, cilium, dynein, infectious disease, microbiology, motility, parasite, trypanosome, Vector-Borne Diseases, Infectious Diseases, 2.2 Factors relating to the physical environment, Infection, Biochemistry and Cell Biology
Abstract

The 96-nm axonemal repeat includes dynein motors and accessory structures as the foundation for motility of eukaryotic flagella and cilia. However, high-resolution 3D axoneme structures are unavailable for organisms among the Excavates, which include pathogens of medical and economic importance. Here we report cryo electron tomography structures of the 96-nm repeat from Trypanosoma brucei, a protozoan parasite in the Excavate lineage that causes African trypanosomiasis. We examined bloodstream and procyclic life cycle stages, and a knockdown lacking DRC11/CMF22 of the nexin dynein regulatory complex (NDRC). Sub-tomogram averaging yields a resolution of 21.8 Å for the 96-nm repeat. We discovered several lineage-specific structures, including novel inter-doublet linkages and microtubule inner proteins (MIPs). We establish that DRC11/CMF22 is required for the NDRC proximal lobe that binds the adjacent doublet microtubule. We propose that lineage-specific elaboration of axoneme structure in T. brucei reflects adaptations to support unique motility needs in diverse host environments.

Published
2019

4. Functional Foods: Middle Eastern and Asian Studies

Author

Nasser Aljaafari, Mariam, primary, Yang, Shun-Kai, additional, Abushelaibi, Aisha, additional, Sultan Alhosani, Maryam, additional, Edward van der Graaf, John, additional, Lai, Kok-Song, additional, and Lim, Swee-Hua-Erin, additional

Published
2024
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9. Essential Oils as Potential Antimicrobial Agents

Author

Varijakzhan, Disha, Yang, Shun-Kai, Chong, Chou Min, Akseer, Riaz, Alhosani, Maryam Sultan, Thomas, Warren, Lai, Kok Song, Lim, Swee Hua Erin, Lichtfouse, Eric, Series Editor, Ranjan, Shivendu, Advisory Editor, Dasgupta, Nandita, Advisory Editor, Panwar, Harsh, editor, and Sharma, Chetan, editor

Published
2021
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17. Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins

Author

Kubo, Shintaroh, primary, Black, Corbin, additional, Joachimiak, Ewa, additional, Yang, Shun Kai, additional, Legal, Thibault, additional, Peri, Katya, additional, Khalifa, Ahmad, additional, Ghanaeian, Avrin, additional, Valente-Paterno, Melissa, additional, De Bellis, Chelsea, additional, Huynh, Phuong, additional, Fan, Zhe, additional, Wloga, Dorota, additional, and Bui, Khanh Huy, additional

Published
2022
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28. The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

Author

Khalifa, Ahmad Abdelzaher Zaki, primary, Ichikawa, Muneyoshi, additional, Dai, Daniel, additional, Kubo, Shintaroh, additional, Black, Corbin Steven, additional, Peri, Katya, additional, McAlear, Thomas S, additional, Veyron, Simon, additional, Yang, Shun Kai, additional, Vargas, Javier, additional, Bechstedt, Susanne, additional, Trempe, Jean-François, additional, and Bui, Khanh Huy, additional

Published
2020
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29. Author response: The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

Author

Khalifa, Ahmad Abdelzaher Zaki, primary, Ichikawa, Muneyoshi, additional, Dai, Daniel, additional, Kubo, Shintaroh, additional, Black, Corbin Steven, additional, Peri, Katya, additional, McAlear, Thomas S, additional, Veyron, Simon, additional, Yang, Shun Kai, additional, Vargas, Javier, additional, Bechstedt, Susanne, additional, Trempe, Jean-François, additional, and Bui, Khanh Huy, additional

Published
2020
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34. The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

Author

Khalifa, Ahmad, primary, Ichikawa, Muneyoshi, additional, Dai, Daniel, additional, Kubo, Shintaroh, additional, Black, Corbin, additional, Peri, Katya, additional, McAlear, Thomas S., additional, Veyron, Simon, additional, Yang, Shun Kai, additional, Vargas, Javier, additional, Bechstedt, Susanne, additional, Trempe, Jean-Francois, additional, and Bui, Khanh Huy, additional

Published
2019
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37. Protein expression patterns in hek-Blue™ - Cells treated with Clinacanthus nutans extracts.

Author

Yang, Shun-Kai, Leo, Teik-Kee, Mai, Chun-Wai, Chin, Swee-Yee, Lamasudin, Dhilia, Lim, Swee-Hua-Erin, Tan, Ngai-Paing, and Lai, Kok-Song

Subjects
*REACTIVE oxygen species, *PROTEIN expression, *SODIUM dodecyl sulfate, *GEL electrophoresis, *EXTRACTS
Abstract

Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two-dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK-Blue™-4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK-Blue™-4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin-1 was also upregulated in HEK-Blue™-4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK-Blue™-4 cells and induced the expression of peroxiredoxin-1, which protects the cells from reactive oxygen species during the inflammation process. [ABSTRACT FROM AUTHOR]

Published
2020
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41. Antibacterial Activity and Mode of Action of ß-caryophyllene on Bacillus cereus

Author

MOO, CHEW-LI, YANG, SHUN-KAI, OSMAN, MOHD-AZURAIDI, YUSWAN, MOHD HAFIS, LOH, JIUN-YAN, LIM, WEI-MENG, LIM, SWEE-HUA-ERIN, and LAI, KOK-SONG

Abstract

Natural products such as essential oils (EOs) are secondary metabolites that can be obtained from either plant or animal sources or produced by microorganisms. Much attention has been given to exploring the use of secondary metabolites as natural antibacterial agents. This study investigates the antibacterial activity and mechanism of ß-caryophyllene, a compound that can be found in various EOs, against Bacillus cereus. The minimum inhibitory concentration of ß-caryophyllene against B. cereuswas 2.5% (v/v), whereas killing kinetics of ß-caryophyllene at minimum inhibitory concentration recorded complete bactericidal activity within 2 hours. Zeta-potential measurement in the cells treated with half the minimum inhibitory concentration of ß-caryophyllene at 1.25% (v/v) showed an increase in the membrane permeability surface charge to –3.98 mV, compared to untreated cells (–5.46 mV). Intracellular contents leakage of UV-absorbing materials was detected in the cells treated with ß-caryophyllene. Additionally, ß-caryophyllene does not interfere with the efflux activity of B. cereusvia the ethidium bromide influx/efflux activity. The results revealed that ß-caryophyllene was able to alter membrane permeability and integrity of B. cereus, leading to membrane damage and intracellular content leakage, which eventually caused cell death.

Published
2020
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45. Performance Enhancement in N 2 Plasma Modified AlGaN/AlN/GaN MOS-HEMT Using HfAlO X Gate Dielectric with Γ-Shaped Gate Engineering.

Author

Yang, Shun-Kai, Mazumder, Soumen, Wu, Zhan-Gao, and Wang, Yeong-Her

Subjects
*GALLIUM nitride, *MODULATION-doped field-effect transistors, *PASSIVATION, *METAL oxide semiconductors, *DIELECTRICS, *SURFACE preparation
Abstract

In this paper, we have demonstrated the optimized device performance in the Γ-shaped gate AlGaN/AlN/GaN metal oxide semiconductor high electron mobility transistor (MOS-HEMT) by incorporating aluminum into atomic layer deposited (ALD) HfO2 and comparing it with the commonly used HfO2 gate dielectric with the N2 surface plasma treatment. The inclusion of Al in the HfO2 increased the crystalline temperature (~1000 °C) of hafnium aluminate (HfAlOX) and kept the material in the amorphous stage even at very high annealing temperature (>800 °C), which subsequently improved the device performance. The gate leakage current (IG) was significantly reduced with the increasing post deposition annealing (PDA) temperature from 300 to 600 °C in HfAlOX-based MOS-HEMT, compared to the HfO2-based device. In comparison with HfO2 gate dielectric, the interface state density (Dit) can be reduced significantly using HfAlOX due to the effective passivation of the dangling bond. The greater band offset of the HfAlOX than HfO2 reduces the tunneling current through the gate dielectric at room temperature (RT), which resulted in the lower IG in Γ-gate HfAlOX MOS-HEMT. Moreover, IG was reduced more than one order of magnitude in HfAlOX MOS-HEMT by the N2 surface plasma treatment, due to reduction of N2 vacancies which were created by ICP dry etching. The N2 plasma treated Γ-shaped gate HfAlOX-based MOS-HEMT exhibited a decent performance with IDMAX of 870 mA/mm, GMMAX of 118 mS/mm, threshold voltage (VTH) of −3.55 V, higher ION/IOFF ratio of approximately 1.8 × 109, subthreshold slope (SS) of 90 mV/dec, and a high VBR of 195 V with reduced gate leakage current of 1.3 × 10−10 A/mm. [ABSTRACT FROM AUTHOR]

Published
2021
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46. Transcriptomic analysis of multi-drug resistant Escherichia coliK-12 strain in response to Lavandula angustifoliaessential oil

Author

Lai, Pey-Jiun, Ng, Ee-Von, Yang, Shun-Kai, Moo, Chew-Li, Low, Wai Yee, Yap, Polly Soo-Xi, Lim, Swee-Hua Erin, and Lai, Kok-Song

Abstract

To better understand the synergistic antibacterial activity between piperacillin and Lavandula angustifoliaessential oil (LEO) against multidrug-resistant Escherichia coli, we performed microarray transcriptomic analysis of LEO when used alone and in combination with piperacillin against the non-treated control. In total, 90 genes were differentially expressed after the combination of LEO and piperacillin treatment. Among the up-regulated genes, nfsB, nemA, fruA, nfsB, nemAare known to control microbial metabolism and nitrotoluene degradation, which were observed only in the LEO–piperacillin combinatory treatment. Four candidate genes from the microarray result, srIA, srID, waaR and nfsB,were validated by qRT-PCR as these genes showed differential expression consistently in the two methods. Biochemical pathway analysis showed that there was upregulation of genes involved in several biological processes including fructose and mannose metabolism, phosphotransferase system (PTS), lipopolysaccharide biosynthesis and nitrotoluene degradation. Genes involved in microbial metabolism in diverse environments were found both up- and down-regulated in LEO–piperacillin combinatory treatment. Our study provides new information concerning the transcriptional changes that occur during the LEO and piperacillin interaction against the multidrug-resistant bacteria and contributes to unravel the mechanisms underlying this synergism.

Published
2020
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48. Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism.

Author

Ghanaeian A, Majhi S, McCaffrey CL, Nami B, Black CS, Yang SK, Legal T, Papoulas O, Janowska M, Valente-Paterno M, Marcotte EM, Wloga D, and Bui KH

Abstract

Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein regulatory complex (N-DRC), which links adjacent doublet microtubules and regulates and coordinates the activity of outer doublet complexes. Despite its critical role in cilia motility, the assembly and molecular basis of the regulatory mechanism are poorly understood. Here, utilizing cryo-electron microscopy in conjunction with biochemical cross-linking and integrative modeling, we localized 12 DRC subunits in the N-DRC structure of Tetrahymena thermophila . We also found that the CCDC96/113 complex is in close contact with the N-DRC. In addition, we revealed that the N-DRC is associated with a network of coiled-coil proteins that most likely mediates N-DRC regulatory activity.

Published
2023
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