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5. SFMD: A Semi-supervised Framework for Pre-trained Language Models Fine-Tuning with Noisy Samples

Author

Yang, Yiwen, Duan, Pengfei, Li, Yongbing, Zhang, Yifang, Xiong, Shengwu, Goos, Gerhard, Series Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Huang, De-Shuang, editor, Si, Zhanjun, editor, and Zhang, Qinhu, editor

Published
2024
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33. Study of STAT3 interactions with DNA as a target for anticancer drug discovery

Author

Yang, Yiwen

Abstract

STAT3 is a transcription factor involved in the regulation of many cellular functions including proliferation, differentiation, apoptosis, migration and immune response. It has great biological value in understanding the regulation system from genes to cellular activities. STAT3, is one transcriptional activator, but is involved in multiple cellular activities in different cells. It is regarded as an important anti-cancer drug target and a biomarker for tumour growth. Understanding the detailed biological activity of STAT3 can lead to the discovery of mechanisms of regulation of different cellular functions. In this thesis, we reveal more detailed biological activities of STAT3 with three different in vitro methods: protein electrophoresis mobility shift assay (PEMSA), fluorescent polarization (FP) and fluorescent resonance energy transfer (FRET). The development of in vitro methods not only provide more direct visualization of STAT3 biological functions such as DNA binding activity, dimerization, and aggregation, but also can be easily developed into inhibitor screening methods. We successfully created and purified different STAT3 mutations that provide different STAT3 functional domains to detect detailed STAT3 domain functions. Both PEMSA assay and FP assay suggest that STAT3 binds to dsDNA without a requirement for the SH2 domain, which emphasis the possibility of STAT3 binding to dsDNA as a monomer. The PEMSA assay provided an intuitive and orthogonal method to detect STAT3 DNA binding activity while the FP assay is very highthroughput. The developed FRET assay can not only be used to study the DNA binding activity of STAT3 but also be used to detect STAT3 dimerization.

Published
2021

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