Search

Abstract UFMylation is a ubiquitination-like modification that is related to endoplasmic reticulum stress and unfolded protein response. A recent study reported that Ufl1, a key enzyme of UFMylation, protects against heart failure, indicating that UFMylation may be associated with heart function regulation. In the present study, we initially constructed a Flag-6×His-tagged Ufm1ΔSC transgenic (Tg-Ufm1) mouse model that enables UFMylation studies in vivo. Tg-Ufm1 mice showed significant activation of UFMylation in hearts. By using this model, we identified 38 potential Ufm1-binding proteins in heart tissues through LC‒MS/MS methods. We found that these proteins were associated with mitochondria, metabolism and chaperone binding. By using transcriptomic screening, we identified Tnfaip2 as a novel UFMylation-associated gene. Overexpression of Ufm1 significantly upregulated the protein expression of Tnfaip2, whereas isoproterenol treatment decreased Tnfaip2 expression in Tg-Ufm1 mice. These data may provide novel clues for UFMylation in cardiac hypertrophy.

Objective To establish a new program of blood cell processing apparatus (NGL-BBS) for the preparation of washed mixed platelets and to study the effect on biological activity of platelets compared with traditional manual method. Methods Mixed concentrated platelets were separated and prepared from whole blood by white membrane method. Blood cell processing apparatus with new program set (experimental group) and manual method (control group) was used for the preparation of washed mixed platelets. The expression rate of CD62P and CD63 in the two groups of washed mixed platelets was compared by flow-cytometry. Thrombus elastography (TEG) was used to measure and compare the MA value between the two groups. Results The expression rate of CD62P and CD63 in the experimental group was lower than that in the control group (t = 4.11, P < 0.01; t = 10.78, P < 0.01). The TEG MA value of the experimental group was higher than that of the control group (t = 6.67, P < 0.01). Conclusion The present study demonstrates that the use of NGL-BBS for the preparation of washed mixed platelets has a lesser impact on biological activity compared to manual preparation methods. [ABSTRACT FROM AUTHOR]

Twenty lake bottom surface layers mud and water samples in the Qilihai Wetland and the Beidagang Reservoir in Tianjin were taken to do diatoms analysis. More than 6000 diatoms were identified and divided into 117 species and 28 genera. Based on the calculation of DAIpo(Diatom Assemblage to organic water pollution), the water pollution level of the Beidagang Reservoir can be divided into: Xenosaprobic, β -oligosaprobic, α -oligosaprobic, β -mesosaprobic, from clear to slightly cloudy water. The water pollution level of Qilihai wetland can be divided into: extremely oligarchy dirt belt, β oligarchy dirt belt, α oligarchy dirt belt, β Medium dirt belt, that is, the water quality is clear to slightly turbid. The water quality of the Beidagang Reservoir is clearer than that of the Qilihai Wetland. The physical and chemical indexes such as EC, pH, TN, TP and CODMn were determined for the water samples, and the correlation between DAIpo and these measured values was analyzed. The results showed that the degree of water pollution detected was consistent with the conclusion obtained by diatom analysis. The results show that the water quality of the Beidagang Reservoir and the Qilihai Wetland was mildly polluted in the spring and summer of 2019, but the eutrophication is more serious.

The Rh blood grouping system is a critical standardized test in transfusion medicine, especially for the cases related to haemolytic transfusion reactions and neonatal haemolytic disease caused by clinical RhD blood group incompatibility. In the present case report, we presented two cases with the uncommon RHD gene variation RHD * DEL37 . The blood samples of the two subjects were mistakenly identified as RhD-negative through conventional serological testing. Firstly, both blood samples were tested negative for the RhD antigen using traditional tube test and gel microcolumn methods. The phenotyping of RhCE were identified as ccEe and ccee for each sample, respectively. Secondly, genetic analysis was performed using polymerase chain reaction-sequence specific prime (PCR-SSP) which revealed that neither sample belonging to the several common RHD gene variants which was found in Asia. Moreover, they turned out to be positive for the RHD haplotype, which indicated that exons 1-10 on one of the RHD alleles were entirely absent. In addition, a T>C mutation was observed at bases 1154-31 in intron 8 of the other allele, which was located at the intron 8 breakpoint. This result was obtained after further Sanger sequencing of exons 1-10 of the RHD gene. The mutant allele was designated as RHD * DEL37 by the International Society of Blood Transfusion (ISBT) and was identified as D-elute(D el ) by phenotype ana-lysis. Both samples were genotyped as RHD * DEL37 and showed positive results. In summary, the true genotype of the two blood samples, of which the screening results only using serological testing method was negative, were RHD * DEL37 / RHD- ( RHD * 01N.01 ). Notably, this kind of genotype was reported for the first time in Chinese population. Moreover, the two individuals did not have ties of consanguinity, indicating that some of the Chinese individuals could be carriers of the genetic mutation. Therefore, it might be necessary to further confirm the frequency of this mutation in the Chinese population and the possibility of homozygosity for this mutation. This report identifies infrequent RHD gene mutation samples by coupling molecular biology and serological methods to prevent misclassification of blood groups. Combining serological and molecular biology test results to determine blood group is critical in protecting patients during clinical transfusion procedures.