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In this study, the modified preparation method of combining planetary and vibratory ball milling was proposed to prepare Mg based hydrogen storage alloy powders. The comparison of micromorphology and hydrogen storage behaviour between Mg2Ni prepared using the modified and conventional preparation methods were investigated experimentally. The comparison results showed that the combination of first planetary and then vibratory ball milling has more favourable effect on improving both the kinetics and the thermodynamics of ball milled Mg2Ni alloys. The sample synthesised by first planetary milling for 40 h and then vibratory milling for 30 h has faster hydrogen absorption kinetics and lower dehydriding onset temperature than those prepared by the single method of planetary or vibratory milling and hydriding combustion synthesis owing to its popcorn-like microstructure. Moreover, this kind of modified method reduces the reaction enthalpy and activation energy by up to ∼18 and 22% respectively. [ABSTRACT FROM AUTHOR]

To determine the alterations of the p16/MTS1 gene in oral squamous cell carcinoma (OSCC), we examined in Taiwanese patients the mutation, deletion and methylation of p16/MTS1 in primary OSCCs associated mostly with betel quid (BQ)/tobacco use. Among 110 tumors undergoing mutational analyses, seven (6%) showed mutations in exon 2 or the intron 1/exon 2 splice site. All but one mutation disrupted the encoded proteins. Base transitions represented the vast majority (6/7) of the mutations identified in BQ/tobacco consuming subjects. It was noted that 15/56 (27%) tumors examined by restriction fragment methylation analysis revealed a significant level of methylation in different loci of exon 1 as compared with the respective non-cancerous tissue. Mutation of p16/MTS1 was exclusively identified in carcinomas of buccal mucosa, whereas methylation of the p16/MTS1 promoter region occurred preferentially in carcinomas of the tongue (54%) rather than at other sites (22%). Homozygous deletion was not found in 56 paired samples examined, nor was hemizygous deletion indicated in 12 informative cases. The results indicated aberrant methylation and mutation as the molecular abnormality of p16/MTS1 in the OSCC from Taiwanese. [ABSTRACT FROM AUTHOR]

Background: Contrast-medium extravasation injuries may be self-limited to catastrophic. Adequate prophylactic measures are enforced when risk factors for extravasation are present, and prompt treatment can avoid serious complications. Purpose: To describe the squeeze maneuver, an effective method for the treatment of symptomatic contrast-medium extravasation. Material and Methods: Over a 3-month period, eight patients with >50 ml contrast-medium extravasation resulting in vascular compromise of the fingers were managed with the squeeze maneuver as follows. The intravenous catheter used for contrast-medium injection was removed, and the skin around the insertion site was cleaned with povidone-iodine. An 18-gauge needle was then used to puncture five to eight openings near the catheter insertion site as deeply as possible. We then began squeezing from the periphery of the swelling toward the needle holes. As the contrast medium drained, it was swabbed away with iodine-soaked cotton swabs. Results: In all eight patients, the maneuver was successful with immediate resolution of the vascular compromise. Conclusion: The squeeze maneuver provides an easy way to manage radiological contrast-medium extravasation and can be performed immediately in the CT suite. [ABSTRACT FROM AUTHOR]

Currently, the 'gold standard' for diagnosis of Psoroptes ovis infections is detecting Psoroptes mites or eggs in skin scrapings under microscopy, but it is prone to be mis-diagnosed for detecting early infection of P. ovis. Hence, seeking a reliable diagnostic technique for detecting early-stage mite infections is extremely desirable. Enzyme linked immunosorbent assay (ELISA) has proven to be useful for the diagnosis of early-stage P. ovis infection. Thus, the purpose of this study was to screen serodiagnostic candidate antigens that can detect early P. ovis infection. Psoroptes ovis var. cuniculi wash proteins (PsoWA), which contained an enriched source of secretory and excretory antigens, were separated by two-dimensional gel electrophoresis (2-DE) and screened by immunoblot using sera from rabbits with early-stage Psoroptes infection (1 week and 3 weeks). Immunogenic proteins were submitted for sequencing by liquid chromatography tandem-mass spectrometry (LC-MS/MS) analyses. Three potential diagnostic antigens were identified (PsoSP3, Pso14-3-3(1) and Pso14-3-3(2)) in this study. These were further expressed in E. coli expression system to evaluate the serodiagnostic potential of these recombinant proteins for detecting early-stage P. ovis infection using an indirect ELISA (iELISA). Western blotting showed that 34 protein spots were recognized by rabbit sera of 1 week post-infection (wpi) and 3 wpi. The 2-DE results showed that a total of 199 proteins were detected with molecular weights varying from 20 to 100 kDa and isoelectric point (pI) from 4.1 to 9.3. Among these, 90 proteins were detected both at 1 wpi sera and 3 wpi sera, and the numbers of the specific identified proteins were 27 for 1 wpi sera and 82 for 3wpi sera. Moreover, rPsoSP3 showed better diagnostic efficacy than rPso14-3-3(1) and rPso14-3-3(2) in detecting early-stage P. ovis infection for its higher values of sensitivity, specificity and area under the receiver operating characteristic curve. Our study describes the first immunoproteomic analysis to identify early diagnostic candidate antigens of P. ovis, and the identified antigens of Psoroptes in our study have significant implications for the development of early-stage diagnostic tests. PsoSP3 is a promising early diagnostic antigen for detecting P. ovis var. cuniculi infection., Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests., (Copyright © 2024. Published by Elsevier B.V.)

Psoroptes mites are the common ecto-parasites of wild and domestic animals worldwide, which causes considerable economic losses in livestock industry. Microscopy is deemed to be the 'gold standard' for the diagnosis of Psoroptes mite infection but it has poor sensitivity for low mite infections and/or sub-clinical infections. To overcome these shortcomings, we screened four genes to develop a sensitive and specific PCR for the detection of Psoroptes mite infection in rabbits, and confirmed its practicability in detecting early infection and monitoring treatment outcome with traditional microscopy and serological tests. Results showed that PCR assay targeting ITS2 (ITS2-PCR) had a high specificity and sensitivity (detection limit: 40.3 pg/μL DNA) for detecting P. ovis DNA. In rabbits artificially infected with P. ovis, all three diagnostic tests showed the same detection rate from 14 days post infection (dpi) to 42 days dpi. However, these diagnostic tests behave differently at 7 dpi and after treatment: at 7 dpi, the detection rate of ITS2-PCR was higher than rPsoSP3-based iELISA and traditional microscopy (ITS2-PCR: 88.9%, rPsoSP3-iELISA: 77.7%, microscopy: 33.3%); at 7 days post treatment (dpt), positivity rates of ITS2-PCR and microscopy rapidly decreased to 0.00% and 11.1%, whereas rPsoSP3-iELISA remained 100% positive rate. Furthermore, the comprehensive comparisons of diagnostic performance and features of three diagnostic tests at 7 dpi were performed. Compared to ITS2-PCR or rPsoSP3-iELISA, microscopy had the lowest sensitivity, and the agreement between these assays was low (κ < 0.3). Field study showed that ITS2-PCR showed a higher detection rate than microscopy (19.4% and 11.1%, respectively). Our results suggested that the ITS2-PCR developed in this study provided a new laboratory tool for diagnosis of P. ovis var. cuniculi infection, and it had advantages over microscopic examination in detection low-level mite infections and serological assay in monitoring treatment outcome., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

The MR imaging findings of a leiomyosarcoma arising from the nasopharynx are presented. To our knowledge, this is the first MR imaging description of this entity.

A systems model of spinal neuro-musculo-skeletal system (alpha - gamma model) is developed to investigate the plausible roles of spinal proprioceptive feedback in movement control. The model is composed of a joint, a pair of antagonist muscles, length and velocity feedback from muscle spindle, as well as spinal stretch reflex, reciprocal inhibition and recurrent inhibition of Renshaw cells. A descending command modulates the background activation of alpha motoneuron pools in combination with these reflex activities. A static gamma command controls the fusimotor contraction of the spindle. Simulation results reveal that the equilibrium joint angle is linearly correlated to the level of static gamma fusimotor activity of the spindle for a wide range of external loading conditions and reflex gains, suggesting that these spinal reflexes may contribute to regulate the equilibrium position of the joint. Sensitivity analysis further shows that reflex gains and other central commands alter the quasi-linear relation in regular fashions. The reciprocal inhibition gain changes the slope of the linear theta(eq) - gamma curve; and the descending alpha excitation, the stretch reflex gain, and the external load all shift the theta(eq) - gamma curve in parallel. These results imply that reflex gains and descending alpha commands may be coordinated to maintain a unique theta(eq) - gamma curve while providing the flexibility to counteract external loads, to execute a movement, or to regulate additional muscle variables. Dynamic simulation suggests that control of a class of movements can be achieved with a triphasic, alpha pulse and a continuous gamma signal. The model study supports the notion of a dual strategy for controlling trajectories via a feedforward alpha command and for regulating the final equilibrium positions via a feedback gamma command.

Percutaneous stricture dilatation and cholangioscopic lithotomy has become a mainstay in the treatment of patients with recurrent hepatolithiasis associated with intrahepatic biliary strictures. In a consecutive series of 125 patients who underwent percutaneous management of recurrent hepatolithiasis from 1987 to 1999, there were 15 patients in whom the procedure failed to clear the stones. A second percutaneous transhepatic route was established for subsequent treatment. A reappraisal of its indications and efficacy was done. Treatment through a second route was helpful for patients with bilateral strictures, angulated duct, difficult strictures, large impacted stones, a subcutaneous jejunal limb, or hemobilia developing in the first route. Strictures remained impacted in 1 of the 15 patients (failure rate, 7%), with the remaining having complete clearance of stones. Cholangitis occurred in two patients; no other complications were encountered. A second percutaneous route is very helpful for the management of complicated hepatolithiasis and biliary stricture.

For recurrent hepatolithiasis coexisting with a complicated long-segment intrahepatic biliary stricture, repeated surgeries, balloon dilation of the stricture, and external-internal stenting may still fail to solve the problem. We tried using a Gianturco-Rosch metallic Z internal stent (Wilson-Cook Medical, Inc., Bloomington, IN, USA) with the aid of percutaneous transhepatic cholangioscopy (PTCS) to treat such patients. Eight patients had a Z stent placed through a percutaneous transhepatic biliary drainage tract. Immediately after stent placement, PTCS was inserted via the percutaneous transhepatic biliary drainage route and a part of the wire skirt not firmly anchored in one of the eight patients was detected. It was successfully repositioned using PTCS. Recurrent cholangitis developed in three patients 6, 7, and 30 months, respectively, after stent placement. PTCS was undertaken again through a reestablished percutaneous transhepatic biliary drainage route and revealed sludge in their stent lumens. We cleared it by PTCS. No further cases of cholangitis occurred in later follow-up. PTCS is useful in ensuring adequate stent position, diagnosing and treating the causes of recurrent cholangitis, and prolonging the function of stents.

A 2-year-10-month-old boy was diagnosed with a complex congenital heart disease: right atrial isomerism, left superior vena cava (LSVC), complete atrioventricular septal defect, secundum type atrial septal defect, transposition of the great arteries with pulmonary atresia, patent ductus arteriosus, absence of a right superior vena cava (RSVC), and dextrocardia. He had received a left Blalock-Taussig (BT) shunt at the age of 3 months and a left bidirectional Glenn shunt one year after BT shunt. Progressive cyanosis was noted after the second operation and cardiac catheterization showed a functional Glenn shunt with an engorged azygos vein, which was inadvertently skipped for ligation. Because of the absence of RSVC, transcatheter occlusion of the azygos vein was performed successfully via direct puncture of the innominate vein.