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5 results on '"Yang-Meng Feng"'

1. Identification and analysis of B cell epitopes of hemagglutinin of H1N1 influenza virus

Author

Qing Feng, Xiao-Yan Huang, Yang-Meng Feng, Li-jun Sun, Jing-Ying Sun, Yan Li, Xin Xie, Jun Hu, and Chun-Yan Guo

Subjects
Mice, Inbred BALB C, Antibodies, Monoclonal, Hemagglutinin Glycoproteins, Influenza Virus, General Medicine, Antibodies, Viral, Biochemistry, Microbiology, Mice, Hemagglutinins, Influenza A Virus, H1N1 Subtype, Genetics, Animals, Epitopes, B-Lymphocyte, Computer Simulation, Molecular Biology
Abstract

The frequent variation of influenza virus hemagglutinin (HA) antigen is the main cause of influenza pandemic. Therefore, the study of B cell epitopes of HA is of great significance in the prevention and control of influenza virus. In this study, the cleavage vaccine of 2009 H1N1 influenza virus was used as immunogen, and the monoclonal antibodies (mAbs) were prepared by conventional hybridoma fusion and screening techniques. The characteristics of mAbs were identified by ELISA method, Western-blot test and hemagglutination inhibition test (HI). Using the obtained mAbs as a tool, the B cell epitopes of HA were predicted by ELISA blocking test, sandwich ELISA method and computer simulation method. Finally, four mAbs against HA antigen of H1N1 influenza virus were obtained. The results of ELISA and computer prediction showed that there were at least two types of epitopes on HA of influenza virus. This study provides experimental data for improving the prediction of HA epitopes of influenza viruses and developing potential influenza vaccines, and also provides a new method for the prediction of other pathogenic microorganisms.

Published
2022
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3. Sinomenine promotes differentiation of induced pluripotent stem cells into immature dendritic cells with high induction of immune tolerance

Author

Xiao-Yan Huang, Zhan-Kui Jin, Meng Dou, Bing-Xuan Zheng, Xiang-Rong Zhao, Qing Feng, Yang-Meng Feng, Xiang-Long Duan, Pu-Xun Tian, and Cui-Xiang Xu

Subjects
Histology, Genetics, Cell Biology, Molecular Biology, Genetics (clinical)
Abstract

Immature dendritic cells (imDCs) play an important role in the induction of donor-specific transplant immunotolerance. However, these cells have limitations, such as rapid maturation and a short lifespanTo study the capacity of SN to maintain iPSC-derived imDCs (SN-iPSCs-imDCs) in an immature state and the mechanism by which SN-iPSCs-imDCs induce immunotolerance.In this study, mouse iPSCs were induced to differentiate into imDCs in culture medium without or with SN (iPSCs-imDCs and SN-iPSCs-imDCs). The imDC-related surface markers, endocytotic capacity of fluorescein isothiocyanate-Dextran and apoptosis were analyzed by flow cytometry. The effects of iPSCs-imDCs and SN-iPSCs-imDCs on T-cell stimulatory function, and regulatory T (Treg) cell proliferative functionBoth iPSCs-imDCs and SN-iPSCs-imDCs were successfully obtained, and their biological characteristics and ability to induce immunotolerance were compared. SN-iPSCs-imDCs exhibited higher CD11c levels and lower CD80 and CD86 levels compared with iPSCs-imDCs. Reduced major histocompatibility complex II expression, worse T-cell stimulatory function, higher Treg cell proliferative function and stronger endocytotic capacity were observed with SN-iPSCs-imDCs (This study demonstrated that SN-iPSCs-imDCs have potential applications

Published
2022

5. Development and validation study of a non-alcoholic fatty liver disease risk scoring model among adults in China.

Author

Qian Zhang, Wong, Carlos K. H., Kenny Kung, Chan, Joseph C. Y., Sy, Barre T. W., Lam, Marcus, Xiang Gui Xu, Meng Feng Yang, Yang Yu, Xiu Ping Lin, Lam, Cindy L. K., Zhang, Qian, Kung, Kenny, Xu, Xiang Gui, Yang, Meng Feng, Yu, Yang, and Lin, Xiu Ping

Subjects
FATTY liver, THERAPEUTICS, DIAGNOSTIC ultrasonic imaging, PRIMARY care, CHRONIC diseases, PATIENTS
Abstract

Background: Non-alcoholic fatty liver disease (NAFLD) is one of the most common liver diseases in China. It is usually asymptomatic and transabdominal ultrasound (USS) is the usual means for diagnosis, but it may not be feasible to have USS screening of the whole population.Objective: To develop a risk scoring model for predicting the presence of NAFLD using parameters that can be easily obtain in clinical settings.Methods: A retrospective study on the data of 672 adults who had general health check including a transabdominal ultrasound. Fractional polynomial and multivariable logistic regressions of sociodemographic and biochemical variables on NAFLD were used to identify the predictors. A risk score was assigned to each predictor using the scaled standardized β-coefficient to create a risk prediction algorithm. The accuracy for NAFLD detection by each cut-off score in the risk algorithm was evaluated.Results: The prevalence of NAFLD in our study population was 33.0% (222/672). Six significant factors were selected in the final prediction model. The areas under the curve (AUC) was 0.82 (95% CI: 0.78-0.85). The optimal cut-off score, based on the ROC was 35, with a sensitivity of 76.58% (95% CI: 70.44-81.98%) and specificity of 74.89% (95% CI: 70.62-78.83%).Conclusion: A NAFLD risk scoring model can be used to identify asymptomatic Chinese people who are at risk of NAFLD for further USS investigation. [ABSTRACT FROM AUTHOR]

Published
2017
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