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The numbers of patients with inflammatory bowel disease (IBD), such as ulcerative colitis (UC) and Crohn’s disease (CD), have been increasing over time, worldwide; however, the pathogenesis of IBD is multifactorial and has not been fully understood. Myosin light chain 9 and 12a and 12b (Myl9/12) are known as ligands of the CD69 molecule. They create “Myl9 nets” that are often detected in inflamed site, which play a crucial role in regulating the recruitment and retention of CD69-expressing effector cells in inflamed tissues. We demonstrated the strong expression of Myl9/12 in the inflamed gut of IBD patients and mice with DSS-induced colitis. The administration of anti-Myl9/12 Ab to mice with DSS-induced colitis ameliorated the inflammation and prolonged their survival. The plasma Myl9 levels in the patients with active UC and CD were significantly higher than those in patients with disease remission, and may depict the disease severity of IBD patients, especially those with UC. Thus, our results indicate that Myl9/12 are involved in the pathogenesis of IBD, and are likely to be a new therapeutic target for patients suffering from IBD.

While IFNγ is a well-known cytokine that actively promotes the type I immune response, it is also known to suppress the type II response by inhibiting the differentiation and proliferation of Th2 cells. However, the mechanism by which IFNγ suppresses Th2 cell proliferation is still not fully understood. We found that IFNγ decreases the expression of growth factor independent-1 transcriptional repressor (GFI1) in Th2 cells, resulting in the inhibition of Th2 cell proliferation. The deletion of the Gfi1 gene in Th2 cells results in the failure of their proliferation, accompanied by an impaired cell cycle progression. In contrast, the enforced expression of GFI1 restores the defective Th2 cell proliferation, even in the presence of IFNγ. These results demonstrate that GFI1 is a key molecule in the IFNγ-mediated inhibition of Th2 cell proliferation.

After spinal cord injury (SCI), astrocytes become hypertrophic, and proliferative, forming a dense network of astroglial processes at the site of the lesion. This constitutes a physical and biochemical barrier to axonal regeneration. Mitochondrial fission regulates cell cycle progression; inhibiting the cell cycle of astrocytes can reduce expression levels of axon growth-inhibitory molecules as well as astroglial scar formation after SCI. We therefore investigated how an inhibitor of mitochondrial fission, Mdivi-1, would affect astrocyte proliferation, astroglial scar formation, and axonal regeneration following SCI in rats. We stern blot and immunofluorescent double-labeling showed that Mdivi-1 markedly reduced the expression of the astrocyte marker glial fibrillary acidic protein (GFAP), and a cell proliferation marker, proliferating cell nuclear antigen, in astrocytes 3 days after SCI. Moreover, Mdivi-1 decreased the expression of GFAP and neurocan, a chondroitin sulfate proteoglycan. Notably, immunofluorescent labeling and Nissl staining showed that Mdivi-1 elevated the production of growth-associated protein-43 and increased neuronal survival at 4 weeks after SCI. Finally, hematoxylin-eosin staining, and behavioral evaluation of motor function indicated that Mdivi-1 also reduced cavity formation and improved motor function 4 weeks after SCI. Our results confirm that Mdivi-1 promotes motor function after SCI, and indicate that inhibiting mitochondrial fission using Mdivi-1 can inhibit astrocyte activation and astroglial scar formation and contribute to axonal regeneration after SCI in rats. [ABSTRACT FROM AUTHOR]

In the field of corrosion protection coatings, layered double hydroxide (LDH) has gained wide attention as a novel controlled-release nanocontainer. In this paper, by using a co-precipitation to store corrosion inhibitors in layered double hydroxide with barrier properties, an environmentally friendly gallic acid (GA) intercalated layered double hydroxide corrosion protection filler (GA-LDH) was prepared. The epoxy coating was then modified with GA-LDH to improve its corrosion protection performance. The structure, composition, and release behavior of GA-LDH were investigated by a series of characterizations, such as field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and ultraviolet-visible spectrophotometry (UV-vis). Electrochemical impedance spectroscopy (EIS) and a neutral salt spray test (NSS) were performed to evaluate the effect of EP coating containing GA-LDH on corrosion protection for Q235 steel. The results show that GA-LDH added to an epoxy coating can achieve excellent corrosion protection performance and is expected to be widely used in marine corrosion protection contexts. [ABSTRACT FROM AUTHOR]

Cancer immunotherapy utilizes our immune system to attack cancer cells and is an extremely promising strategy for cancer treatment. Although immune-checkpoint blockade, such as anti-PD-1 (programmed cell death 1) antibody, has demonstrated significant enhancement of anti-tumor immunity and has induced notable clinical outcomes, its response rates remain low, and adverse effects are always a matter of concern; therefore, new targets for cancer immunotherapy are always desired. In this situation, new concepts are needed to fuel the investigation of new target molecules for cancer immunotherapy. We propose that CD69 is one such target molecule. CD69 is known to be an activation marker of leukocytes and is also considered a crucial regulator of various immune responses through its interacting proteins. CD69 promotes T-cell retention in lymphoid tissues via sphingosine-1-phosphate receptor 1 (S1P1) internalization and also plays roles in the pathogenesis of inflammatory disorders through interacting with its functional ligands Myl9/12 (myosin light chains 9, 12a and 12b). In anti-tumor immunity, CD69 is known to be expressed on T cells in the tumor microenvironment (TME) and tumor-draining lymph nodes (TDLNs). We revealed that CD69 negatively regulates the effector function of intratumoral T cells and importantly controls the 'exhaustion' of CD8 T cells. In addition, we and others showed that either CD69 deficiency or the administration of anti-CD69 monoclonal antibody enhances anti-tumor immunity. Thus, CD69 is an attractive target for cancer immunotherapy. [ABSTRACT FROM AUTHOR]

Electronic Health Records are a digital version of paper -based records. Studying previous treatment, care and medications is important for making diagnosis for the current situation. The aim of this paper relates to the use of EHR as a means of communication between patient and health care provider with a focus on how EHRs communicates health information for unconscious patient to physicians. As accessing records requires credential s of patient and if the patient is not in a condition to enter his credentials, this leads to the scope of dynamic access control. Dynamic access control is provided by dividing EHRs into different levels. The basis for these levels is physician's specialization and patient's health status. This framework is implemented with the help of a WAMP server using PHP and MySQL. [ABSTRACT FROM AUTHOR]

While IFNγ is a well-known cytokine that actively promotes the type I immune response, it is also known to suppress the type II response by inhibiting the differentiation and proliferation of Th2 cells. However, the mechanism by which IFNγ suppresses Th2 cell proliferation is still not fully understood. We found that IFNγ decreases the expression of growth factor independent-1 transcriptional repressor (GFI1) in Th2 cells, resulting in the inhibition of Th2 cell proliferation. The deletion of the Gfi1 gene in Th2 cells results in the failure of their proliferation, accompanied by an impaired cell cycle progression. In contrast, the enforced expression of GFI1 restores the defective Th2 cell proliferation, even in the presence of IFNγ. These results demonstrate that GFI1 is a key molecule in the IFNγ-mediated inhibition of Th2 cell proliferation. [ABSTRACT FROM AUTHOR]

The numbers of patients with inflammatory bowel disease (IBD), such as ulcerative colitis (UC) and Crohn's disease (CD), have been increasing over time, worldwide; however, the pathogenesis of IBD is multifactorial and has not been fully understood. Myosin light chain 9 and 12a and 12b (Myl9/12) are known as ligands of the CD69 molecule. They create "Myl9 nets" that are often detected in inflamed site, which play a crucial role in regulating the recruitment and retention of CD69-expressing effector cells in inflamed tissues. We demonstrated the strong expression of Myl9/12 in the inflamed gut of IBD patients and mice with DSS-induced colitis. The administration of anti-Myl9/12 Ab to mice with DSS-induced colitis ameliorated the inflammation and prolonged their survival. The plasma Myl9 levels in the patients with active UC and CD were significantly higher than those in patients with disease remission, and may depict the disease severity of IBD patients, especially those with UC. Thus, our results indicate that Myl9/12 are involved in the pathogenesis of IBD, and are likely to be a new therapeutic target for patients suffering from IBD. [ABSTRACT FROM AUTHOR]

The Late Triassic igneous rocks in the Yidun terrane can provide vital insights into the evolution of Plaeo‐Tethys in western China. We present new zircon U–Pb, whole‐rock geochemistry, and Sr–Nd–Pb–Hf isotopic data for the Litang biotite monzogranites, Yidun terrane. The biotite monzogranites have a zircon U‐Pb age of 206.1±1.0 Ma (MSWD=1.9, n=30), which indicates Late Triassic magmatism. The biotite monzogranites display I‐type affinity, high Na2O (3.38–3.60wt%) contents, medium SiO2(67.12–69.13wt%), and low P2O5 contents (0.10–0.12wt%). They are enriched in Rb, Th, and Ba and depleted in Nb and Ta, with negative Eu anomalies (Eu/Eu∗=0.74–0.81). They have evolved Sr‐Nd‐Pb‐Hf isotopic composition, i.e., (87Sr/86Sr)i=0.714225 to 0.714763, negative εNd(t) values of –2.0 to –2.6 with two‐stage Nd model ages ranging from 1.01 to 1.05 Ga, negative εHf(t) values of –3.4 to –4.1 with two‐stage Hf model ages of 1.85 to 1.88 Ga, suggesting a matured crustal sources. Their low Al2O3/TiO2 ratios and medium Cao/Na2O ratios, medium Mg# and SiO2 contents, low [molar Al2O3/(MgO+FeOT)] values, and high [molar Cao/(MgO+FeOT)] values indicate that the Litang biotite monzogranite was formed by partial melting of metabasaltic rocks. Based on the previous studies, we propose that the Litang biotite monzogranite derived from the westward subduction and closure of the Ganzi–Litang ocean during the Late Triassic. The mantle wedge‐derived mafic melts provided sufficient heat for partial melting of ancient metabasalt protolith within the middle‐lower crust. [ABSTRACT FROM AUTHOR]

The calc-alkaline volcanic magmas, which formed the Mesozoic uraniferous volcanic complex of Xiangshan, resulted from partial melting of the mixture of lower crust and enriched mantle with a high mixing proportion in a specific tectonic setting such as active continental margin or ocean-continent collision zone. The preliminary concentrations of U and Th occur in low-degree partial melts. Only small part of these melts was rapidly extracted and erupted and most intruded into the high-level magma chamber (depth: 12-13 km) of the compressed upper lithosphere, in which occurred a strong differentiation which would resulted in strong preconcentrations of the high-hygromagmaphile elements U and Th associated with strong depletion of the 3-d transition elements Ti, Sc, Co, Zr, etc. At the final stage of subduction of the West-Pacific-Kula plate towards the Asian continental plate, the regional tectonic environment was transformed from a compressive into a tensional setting. The strongly differentiated, U (and Th)-enriched silicic alkalic magmas in high level magma chamber extensively erupted, extruded and intruded. The hydrothermal fluids released as a result of late volcano-degassing and dewatering during crystallization-solidification of magmas, resulted in the remobilization, leaching, migration and reconcentration of uranium, which had been preconcentrated in volcanic rocks. Therefore, specific regional petrogeochemical criteria are expected for the uraniferous volcanic series. [ABSTRACT FROM AUTHOR]

The global COVID-19 pandemic has caused a transformation of virtually all aspects of the world order today. Due to the introduction of the world quarantine, a considerable share of professional communications has been transformed into a format of distance interaction. As a result, the specific weight of traditional components of the investment attractiveness of a region is steadily going down, because modern business can be built without the need for territorial unity. It should be stated that now the criteria according to which investors decide if they are ready to invest in a region are dynamically transforming. The significance of the following characteristics is increasingly growing: the sustainable development of a region, qualities of the social environment, and consistency of the social infrastructure. Thus, the approaches to evaluating the region's investment attractiveness must be transformed. Moreover, the investment process at the federal level involves the determination of target areas of regional development. Despite the universal significance of innovative development, the region can develop much more dynamically when a complex external environment is formed that complements its development model. Interregional interaction, as well as an integrated approach to innovative development, taking into account not only the momentary effect, but also the qualitative long-term transformation of the region, will significantly increase the return on investment. At the same time, the currently existing methods for assessing the investment attractiveness of the region are usually heuristic in nature and are not universal. The heuristic nature of the existing methods does not allow to completely abstract from the subjectivity of the researcher. Moreover, the existing methods do not take into account the cyclical properties of the innovative development of the region, which lead to the formation of a long-term effect from the transformation of the regional environment. This study is aimed at forming a comprehensive methodology that can be used to evaluate the investment attractiveness of a certain region and conclude about the lines of business that should be developed in it as well as to find ways to increase the region's investment attractiveness. According to the results of the study, a comprehensive methodology was formed to evaluate the region's investment attractiveness. It consists of three key indicators, namely, the level of the region's investment attractiveness, the projected level of the region's investment attractiveness, and the development vector of the region's investment attractiveness. This methodology is based on a set of indicators that consider the status of the economic and social environment of the region, as well as the status of the innovative and ecological environment. The methodology can be used to make multi-dimensional conclusions both about the growth areas responsible for increasing the region's innovative attractiveness and the lines of business that should be developed in the region. [ABSTRACT FROM AUTHOR]