48 results on '"Yanlei Sun"'
Search Results
2. Unlocking environmental, social, and governance (ESG) performance through energy efficiency and green tax: SEM-ANN approach
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Yanlei Sun, Md Mominur Rahman, Xu Xinyan, Abu Bakkar Siddik, and Mohammad Ekramol Islam
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Environmental performance ,Social performance ,Governance performance ,Green tax ,Energy efficiency ,ESG performance ,Energy industries. Energy policy. Fuel trade ,HD9502-9502.5 - Abstract
In the realm of sustainable development, Environmental, Social, and Governance (ESG) performance has become a crucial metric for evaluating the long-term viability and ethical conduct of businesses. However, there remains a gap in understanding how these factors operate within the context of developing nations such as Bangladesh. Given the unique socio-economic and environmental challenges faced by such countries, it is imperative to explore the relationship between green tax, energy efficiency, and ESG performance within their manufacturing sectors. Thus, this study investigates direct and mediated effects of green tax and energy efficiency on ESG performance. The study uses 457 responses from manufacturing companies and employs PLS-SEM and ANN model to analyze the data. The results reveal a positive association between green tax policies and ESG performance, indicating that such fiscal measures can contribute to sustainable business practices. Furthermore, energy efficiency initiatives exhibit a positive impact on all dimensions of ESG performance, highlighting their role in fostering environmental stewardship, social responsibility, and effective governance within manufacturing operations. Notably, green tax serves as a mediator between energy efficiency measures and ESG outcomes, emphasizing its intermediary role in driving sustainable practices. This research offers practical insights for policymakers, business leaders, and sustainability practitioners integrating energy efficiency initiatives into green tax policies to enhance ESG performance.
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- 2024
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3. Orchestrated Biosynthesis of the Secondary Metabolite Cocktails Enables the Producing Fungus to Combat Diverse Bacteria
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Yanlei Sun, Bo Chen, Xinlin Li, Ying Yin, and Chengshu Wang
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Metarhizium ,chemical ecology ,master regulator ,secondary metabolism ,supercluster ,Microbiology ,QR1-502 - Abstract
ABSTRACT Fungal secondary metabolites with antibiotic activities can promote fungal adaptation to diverse environments. Besides the global regulator, individual biosynthetic gene clusters (BGCs) usually contain a pathway-specific transcription factor for the tight regulation of fungal secondary metabolism. Here, we report the chemical biology mediated by a supercluster containing three BGCs in the entomopathogenic fungus Metarhizium robertsii. These clusters are jointly controlled by an embedded transcription factor that orchestrates the collective production of four classes of chemicals: ustilaginoidin, indigotide, pseurotin, and hydroxyl-ovalicin. The ustilaginoidin BGC is implicated as a late-acquired cluster in Metarhizium to produce both the bis-naphtho-γ-pyrones and the monomeric naphtho-γ-pyrone glycosides (i.e., indigotides). We found that the biosynthesis of indigotides additionally requires the functions of paired methylglucosylation genes located outside the supercluster. The pseurotin/ovalicin BGCs are blended and mesosyntenically conserved to the intertwined pseurotin/fumagillin BGCs of Aspergillus fumigatus. However, the former have lost a few genes, including a polyketide synthase gene responsible for the production of a pentaene chain used for assembly with ovalicin to form fumagillin, as observed in A. fumigatus. The collective production of chemical cocktails by this supercluster was dispensable for fungal virulence against insects and could enable the fungus to combat different bacteria better than the metabolite(s) produced by an individual BGC could. Thus, our results unveil a novel strategy employed by fungi to manage chemical ecology against diverse bacteria. IMPORTANCE Fungal chemical ecology is largely mediated by the metabolite(s) produced by individual biosynthetic gene clusters (BGCs) with antibiotic activities. We report a supercluster containing three BGCs that are jointly controlled by an embedded master regulator in the insect pathogen Metarhizium robertsii. Four classes of chemicals, namely, ustilaginoidin, indigotide, pseurotin, and hydroxyl-ovalicin, are collectively produced by these three BGCs along with the contributions of tailoring enzyme genes located outside the supercluster. The production of these metabolites is not required for the fungal infection of insect hosts, but it benefits the fungus to combat diverse bacteria. The findings reveal and advocate a “the-more-the-better” strategy employed by fungi to manage effective adaptations to diverse environments.
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- 2022
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4. Production of Helvolic Acid in Metarhizium Contributes to Fungal Infection of Insects by Bacteriostatic Inhibition of the Host Cuticular Microbiomes
- Author
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Yanlei Sun, Song Hong, Haimin Chen, Ying Yin, and Chengshu Wang
- Subjects
chemical biology ,cuticular microbiome ,entomopathogenic fungus ,helvolic acid ,Metarhizium ,bacteriostatic activity ,Microbiology ,QR1-502 - Abstract
ABSTRACT The nortriterpenoid helvolic acid (HA) has potent antibiotic activities and can be produced by different fungi, yet HA function remains elusive. Here, we report the chemical biology of HA production in the insect pathogen Metarhizium robertsii. After deletion of the core oxidosqualene cyclase gene in Metarhizium, insect survival rates were significantly increased compared to those of insects treated with the wild type and the gene-rescued strain during topical infections but not during injection assays to bypass insect cuticles. Further gnotobiotic infection of axenic Drosophila adults confirmed the HA contribution to fungal infection by inhibiting bacterial competitors in an inoculum-dependent manner. Loss of HA production substantially impaired fungal spore germination and membrane penetration abilities relative to the WT and gene-complemented strains during challenge with different Gram-positive bacteria. Quantitative microbiome analysis revealed that HA production could assist the fungus to suppress the Drosophila cuticular microbiomes by exerting a bacteriostatic rather than bactericidal effect. Our data unveil the chemical ecology of HA and highlight the fact that fungal pathogens have to cope with the host cuticular microbiomes prior to successful infection of hosts. IMPORTANCE Emerging evidence has shown that the plant and animal surface microbiomes can defend hosts against fungal parasite infections. The strategies employed by fungal pathogens to combat the antagonistic inhibition of insect surface bacteria are still elusive. In this study, we found that the potent antibiotic helvolic acid (HA) produced by the insect pathogen Metarhizium robertsii contributes to natural fungal infection of insect hosts. Antibiotic and gnotobiotic infection assays confirmed that HA could facilitate fungal infection of insects by suppression of the host cuticular microbiomes through its bacteriostatic instead of bactericidal activities. The data from this study provide insights into the novel chemical biology of fungal secondary metabolisms.
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- 2022
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5. Microbiome assembly on Drosophila body surfaces benefits the flies to combat fungal infections
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Song Hong, Yanlei Sun, Dapeng Sun, and Chengshu Wang
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Entomology ,Microbiome ,Mycology ,Science - Abstract
Summary: In contrast to the well-characterized gut microbiomes, the composition and function of the insect body-surface microbiotas are still elusive and highly underexplored. Here we report the dynamic features of the Drosophila melanogaster surface microbiomes. It was found that the microbiomes assembled on fly surfaces could defend insects against fungal parasitic infections. The substantial increase of bacterial loads occurred within 10 days of fly eclosion, especially the expansion of Gilliamella species. The culturable bacteria such as Lactiplantibacillus plantarum could effectively inhibit fungal spore germinations, and the gnotobiotic addition of the isolated bacteria could substantially delay fungal infection of axenic flies. We found that the fly tarsal segments were largely accumulated with bacterial cells, which could accelerate cell dispersal onto different body parts to deter fungal spore germinations. Our findings will facilitate future investigations of the surface microbiotas affecting insect physiologies.
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- 2022
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6. Inductive Production of the Iron-Chelating 2-Pyridones Benefits the Producing Fungus To Compete for Diverse Niches
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Bo Chen, Yanlei Sun, Shiqin Li, Ying Yin, and Chengshu Wang
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2-pyridone ,tenellin ,biosynthetic regulation ,methylglucosylation ,iron chelation ,niche competition ,Microbiology ,QR1-502 - Abstract
ABSTRACT Diverse 2-pyridone alkaloids have been identified with an array of biological and pharmaceutical activities, including the development of drugs. However, the biosynthetic regulation and chemical ecology of 2-pyridones remain largely elusive. Here, we report the inductive activation of the silent polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) (tenS) gene cluster for the biosynthesis of the tenellin-type 2-pyridones in the insect-pathogenic fungus Beauveria bassiana when cocultured with its natural competitor fungus Metarhizium robertsii. A pathway-specific transcription factor, tenR, was identified, and the overexpression of tenR well expanded the biosynthetic mechanism of 15-hydroxytenellin (15-HT) and its derivatives. In particular, a tandemly linked glycosyltransferase-methyltransferase gene pair located outside the tenS gene cluster was verified to mediate the rare and site-specific methylglucosylation of 15-HT at its N-OH residue. It was evident that both tenellin and 15-HT can chelate iron, which could benefit B. bassiana to outcompete M. robertsii in cocultures and to adapt to iron-replete and -depleted conditions. Relative to the wild-type strain, the deletion of tenS had no obvious negative effect on fungal virulence, but the overexpression of tenR could substantially increase fungal pathogenicity toward insect hosts. The results of this study well advance the understanding of the biosynthetic machinery and chemical ecology of 2-pyridones. IMPORTANCE Different 2-pyridones have been identified, with multiple biological activities but unclear chemical ecology. We found that the silent tenS gene cluster was activated in the insect pathogen Beauveria bassiana when the fungus was cocultured with its natural competitor Metarhizium robertsii. It was established that the gene cluster is regulated by a pathway-specific regulator, tenR, and the overexpression of this transcription factor expanded the biosynthetic machinery of the tenellin 2-pyridones. It was also found that the paired genes located outside the tenS cluster contribute to the site-specific methylglucosylation of the main compound 15-hydroxytenellin. Both tenellin and 15-hydroxytenellin can chelate and sequester iron to benefit the producing fungus to compete for different niches. This study well advances the biosynthetic mechanism and chemical ecology of 2-pyridones.
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- 2021
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7. Low Molecular Weight Fucoidan Can Inhibit the Fibrosis of Diabetic Kidneys by Regulating the Kidney Lipid Metabolism
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Yan Wang, Yanlei Sun, Fengli Shao, Bo Zhang, Zhen Wang, and Xinpeng Li
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Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
In this study, a diabetic kidney disease model was established by placing the test rats on a high-sugar/high-fat diet combined with streptozotocin induction. Histopathological examination (H&E, Masson, and PASM stain) showed pathological changes in the diabetic rat kidneys, in addition to fibrotic symptoms and collagen deposition. Immunohistochemistry and western blot analyses indicated that the diabetic condition significantly increased the expressions of fibrotic markers including collagen, α-SMA, and fibronectin. The levels of cholesterol, triglyceride, and low-density lipoprotein were also increased in diabetic kidney disease (DKD) rat blood, while the level of high-density lipoprotein was decreased. The results of Oil red O staining experiments indicated that the kidneys of diabetic rats exhibited appreciable fat deposition, with high contents of triglyceride and cholesterol. To inhibit fibrosis and reduce fat deposition, low molecular weight fucoidan (LMWF) may be used. Based on PCR and western blot analyses, LMWF can regulate the expression levels of important lipid metabolism regulators, thereby impeding the development of kidney fibrosis. Through the vitro model, it also be indicated that LMWF could inhibit fibrosis process through regulating lipid metabolism which induced by palmitic acid.
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- 2021
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8. Study and Application of Molecularly Imprinted Electrochemical Sensor Based on AuNPs/N-GR@CS for Highly Selective Recognition of Trace Hyperoside
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Bolu Sun, Dan Wu, Yanlei Sun, Hongxia Shi, Lin Yang, Chengyang Gao, Lei Kan, Quhuan Ma, and Xiaofeng Shi
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chitosan functionalized Nitrogen-doped graphene (N-GR@CS) ,molecularly imprinted polymer membranes (MIP) ,trace amount of index component ,high selectivity ,electrochemical detection ,Industrial electrochemistry ,TP250-261 - Abstract
Establishing a high-selectivity and rapid detection technology for trace index components in complex samples is of great significance for real-time and on-site drug quality evaluation. In this study, a molecularly imprinted electrochemical sensor with highly selective recognition and detection of trace hyperoside was prepared using chitosan functionalized Nitrogen-doped graphene composite coated with gold nanoparticles (AuNPs/N-GR@CS) as electrode substrate modification material, and the deposition of AuNPs further improved the conductivity of the modified electrode. With the aid of molecular imprinting technology, polymer films with high selectivity and identification of hyperoside were successfully prepared on glassy carbon electrodes (GCE) by self-assembly using hyperoside as template molecule and acrylamide as functional monomer. Because the acrylamide can accept protons through the olefinic double bond and firmly polymerize with each other, while it binds with hyperoside through hydrogen bonds. Therefore, the hyperoside can be easily dissociated in the eluate, which offers a condition for formating a molecularly imprinted polymer film to highly select hyperoside. The highly conductive N-GR@CS modified at the bottom of the polymer film provides the possibility to electrocatalyze hyperoside, and facilitate electron transfer to amplify the response signal. Under the optimized experimental conditions, the sensor showed a detection limit was 6.42 × 10 ^−8 mol l ^−1 (S/N = 3) with a good linear relationship in the range of 2.15 × 10 ^−7 to 2.15 × 10 ^−5 mol l ^−1 . Moreover, it displayed good reproducibility and stability, and could realize the direct and highly selective detection of trace hyperoside in complex samples. In consequence, this study is expected to provide a convenient and reliable method for on-site real-time evaluation of traditional Chinese medicine (TCM) quality with reference to the index components.
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- 2022
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9. Bioactive Metabolites and Potential Mycotoxins Produced by Cordyceps Fungi: A Review of Safety
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Bo Chen, Yanlei Sun, Feifei Luo, and Chengshu Wang
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Cordyceps fungi ,mass production ,mycotoxins ,biosynthetic gene cluster ,toxicity ,safety ,Medicine - Abstract
Ascomycete Cordyceps fungi such as C. militaris, C. cicadae, and C. guangdongensis have been mass produced on artificial media either as food supplements or health additives while the byproducts of culture substrates are largely used as animal feed. The safety concerns associated with the daily consumption of Cordyceps fungi or related products are still being debated. On the one hand, the known compounds from these fungi such as adenosine analogs cordycepin and pentostatin have demonstrated different beneficial or pharmaceutical activities but also dose-dependent cytotoxicities, neurological toxicities and or toxicological effects in humans and animals. On the other hand, the possibility of mycotoxin production by Cordyceps fungi has not been completely ruled out. In contrast to a few metabolites identified, an array of biosynthetic gene clusters (BGCs) are encoded in each genome of these fungi with the potential to produce a plethora of as yet unknown secondary metabolites. Conservation analysis of BGCs suggests that mycotoxin analogs of PR-toxin and trichothecenes might be produced by Cordyceps fungi. Future elucidation of the compounds produced by these functionally unknown BGCs, and in-depth assessments of metabolite bioactivity and chemical safety, will not only facilitate the safe use of Cordyceps fungi as human food or alternative medicine, but will also benefit the use of mass production byproducts as animal feed. To corroborate the long record of use as a traditional medicine, future efforts will also benefit the exploration of Cordyceps fungi for pharmaceutical purposes.
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- 2020
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10. Achieving Resilient Cities Using Data-Driven Energy Transition: A Statistical Examination of Energy Policy Effectiveness and Community Engagement
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Yanlei, Sun, primary, Xing, Zhanlei, additional, and Liu, Guojun, additional
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- 2023
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11. Performance study of a free-piston Stirling heat pump with a circumferential temperature gradient in the heating heat exchanger
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Riying Wang, Jianying Hu, Zilong Jia, Ercang Luo, Jingyuan Xu, and Yanlei Sun
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Mechanical Engineering ,Building and Construction - Published
- 2023
12. Suppression of the insect cuticular microbiomes by a fungal defensin to facilitate parasite infection
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Song Hong, Yanlei Sun, Haimin Chen, and Chengshu Wang
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Defensins ,Fungal Proteins ,Insecta ,Microbiota ,Parasitic Diseases ,Animals ,Beauveria ,Spores, Fungal ,Microbiology ,Ecology, Evolution, Behavior and Systematics - Abstract
Insects can assemble defensive microbiomes on their body surfaces to defend against fungal parasitic infections. The strategies employed by fungal pathogens to combat host cuticular microbiotas remains unclear. Here, we report the identification and functional characterization of the defensin-like antimicrobial gene BbAMP1 encoded by the entomopathogenic fungus Beauveria bassiana. The mature peptide of BbAMP1 can coat fungal spores and can be secreted by the fungus to target and damage Gram-positive bacterial cells. Significant differences in insect survival were observed between the wild-type and BbAMP1 mutant strains during topical infection but not during injection assays that bypassed insect cuticles. Thus, BbAMP1 deletion considerably reduced fungal virulence while gene overexpression accelerated the fungal colonization of insects compared with the wild-type strain in natural infections. Topical infection of axenic Drosophila adults evidenced no difference in fly survivals between strains. However, the gnotobiotic infections with the addition of Gram-positive but not Gram-negative bacterial cells in fungal spore suspensions substantially increased the survival of the flies treated with ∆BbAMP1 compared to those infected by the wild-type and gene-overexpression strains. Bacterial colony counts and microbiome analysis confirmed that BbAMP1 could assist the fungus to manipulate insect surface bacterial loads. This study reveals that fungal defensin can suppress the host surface defensive microbiomes, which underscores the importance to extend the research scope of fungus-host interactions.
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- 2022
13. An Electrochemical Immunosensor Based on GO@CS for Highly Sensitive and Accurate Detection of Cardiovascular Markers-cTn- I.
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Bolu Sun, Lei Kan, Yuhong Liu, Yanlei Sun, Jinhai Liu, Yuqiong Wu, Huajie Li, Shixing Yu, Liangrong Bao, Chengyang Gao, Yanmei Yang, and Lin Yang
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TECHNOLOGICAL risk assessment ,GOLD nanoparticles ,TROPONIN I ,ELECTRIC conductivity ,DETECTION limit - Abstract
The hidden hazards of cardiovascular diseases (CVDs) are enormous, and there is a lack of early warning and prognostic risk assessment technologies. In this study, Cardiac troponin I (cTn-I), the "gold standard" for CVDs diagnosis, was selected as the target molecule, and chitosan functionalized graphene oxide composites (GO@CS) with excellent electrochemical performance were prepared as the electrode base material. Gold nanoparticles (AuNPs) were deposited on GO@CS by electrochemical deposition. Due to the large surface area, excellent electrical conductivity and good biocompatibility of GO@CS assembled with AuNPs provide abundant active sites for the immobilization of cTn-I antigen. Under the optimized experimental conditions, the fabricated immunosensor had a wide linear range of 2.44 × 10
-2 ng ml-1 to 2.50 × 10 ng ml-1 and a low detection limit of 1.12 × 10-2 ng ml-1 (S/N = 3). This study achieves the highly sensitive identification and detection of trace amounts of cTn-I in complex biological samples, providing a convenient and efficient method for the detection of CVDs-related markers. It also provides a scientific basis, new technologies and new methods for the development of objective early warning techniques for CVDs and the study of related pathological mechanisms. [ABSTRACT FROM AUTHOR]- Published
- 2023
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14. The non-linearity between financial development and carbon footprints: the environmental roles of technological innovation, renewable energy, and foreign direct investment
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Yanlei Sun, Raad Mahmoud Al-Tal, Abu Bakkar Siddik, Samiha Khan, Muntasir Murshed, and Rafael Alvarado
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Economics and Econometrics - Published
- 2023
15. Piston offset inhibition method based on check valve backflow in a linear compressor
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Zhixu Chen, Jianying Hu, Yanlei Sun, Limin Zhang, Yanyan Chen, Xiaotao Wang, Jingyuan Xu, and Ercang Luo
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Mechanical Engineering ,Building and Construction - Published
- 2023
16. Operational characteristics of a free-piston Stirling generator with resonant self-circulating heat exchangers
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Jing Luo, Limin Zhang, Yanlei Sun, Yanyan Chen, Guoyao Yu, Jianying Hu, and Ercang Luo
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Energy Engineering and Power Technology ,Industrial and Manufacturing Engineering - Published
- 2023
17. Evaluating the antioxidant activity of secondary metabolites of endophytic fungi from Hypericum perforatum L. by an electrochemical biosensor based on AuNPs/AC@CS composite
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Bolu Sun, Yanmei Yang, Yanlei Sun, Dan Wu, Lei Kan, Chengyang Gao, Hongxia Shi, Chunyan Sang, Tiankun Zhao, Lin Yang, Quhuan Ma, and Xiaofeng Shi
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Electrochemistry ,Biophysics ,General Medicine ,Physical and Theoretical Chemistry - Published
- 2023
18. A bacterial-like Pictet–Spenglerase drives the evolution of fungi to produce β-carboline glycosides together with separate genes.
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Xin-Lin Li, Yanlei Sun, Ying Yin, Shuai Zhan, and Chengshu Wang
- Subjects
- *
HORIZONTAL gene transfer , *FUNGAL genes , *GLYCOSIDES , *BEAUVERIA bassiana , *GENE families - Abstract
Diverse β-carboline (βC) alkaloids are produced by microbes, plants, and animals with myriad bioactivities and drug potentials. However, the biosynthetic mechanism of βCs remains largely elusive, especially regarding the hydroxyl and glucosyl modifications of βCs. Here, we report the presence of the bacterial-like Pictet–Spenglerase gene Fcs1 in the entomopathogenic Beauveria fungi that can catalyze the biosynthesis of the βC skeleton. The overexpression of Fcs1 in Beauveria bassiana led to the identification of six βC methyl glycosides, termed bassicarbosides (BCSs) A–F. We verified that the cytochrome P450 (CYP) genes adjacent to Fcs1 cannot oxidize βCs. Alternatively, the separated CYP684B2 family gene Fcs2 was identified to catalyze βC hydroxylation together with its cofactor gene Fcs3. The functional homologue of Fcs2 is only present in the Fcs1-containing fungi and highly similar to the Fcs1-connected yet nonfunctional CYP. Both evolved quicker than those from fungi without Fcs1 homologues. Finally, the paired methyl/glucosyl transferase genes were verified to mediate the production of BCSs from hydroxy-βCs. All these functionally verified genes are located on different chromosomes of Beauveria, which is in contrast to the typical content-clustered feature of fungal biosynthetic gene clusters (BGCs). We also found that the production of BCSs selectively contributed to fungal infection of different insect species. Our findings shed light on the biosynthetic mechanism of βC glycosides, including the identification of a βC hydroxylase. The results of this study also propose an evolving process of fungal BGC formation following the horizontal transfer of a bacterial gene to fungi. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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19. Use of Aramid Paper in the Magnetic Pole of Hydro-generator
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Junhua Zhang, Yutian Wang, Yanlei Sun, and Ralph Tropeano
- Published
- 2022
20. Do international trade diversification, intellectual capital, and renewable energy transition ensure effective natural resources management in BRICST region
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Yanlei Sun, Siyao Wang, and Zhanlei Xing
- Subjects
Economics and Econometrics ,Sociology and Political Science ,Management, Monitoring, Policy and Law ,Law - Published
- 2023
21. A displacer coupled thermoacoustic cooler driven by heat and electricity
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Jiangfeng Hu, Jianying Hu, Yanlei Sun, Jingyuan Xu, Zilong Jia, Limin Zhang, Ercang Luo, and Bo Gao
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Energy Engineering and Power Technology ,Industrial and Manufacturing Engineering - Published
- 2023
22. [Retracted] MicroRNA‑137 has a suppressive role in liver cancer via targeting EZH2
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Shichang Cui, Yanlei Sun, Yang Liu, Chengbiao Liu, Jinbao Wang, Guang Hao, and Qidong Sun
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Cancer Research ,Oncology ,Genetics ,Molecular Medicine ,Molecular Biology ,Biochemistry - Published
- 2022
23. Microbiome assembly on
- Author
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Song, Hong, Yanlei, Sun, Dapeng, Sun, and Chengshu, Wang
- Abstract
In contrast to the well-characterized gut microbiomes, the composition and function of the insect body-surface microbiotas are still elusive and highly underexplored. Here we report the dynamic features of the
- Published
- 2021
24. Low Molecular Weight Fucoidan Can Inhibit the Fibrosis of Diabetic Kidneys by Regulating the Kidney Lipid Metabolism
- Author
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Bo Zhang, Yanlei Sun, Xinpeng Li, Yan Wang, Zhen Wang, and Fengli Shao
- Subjects
medicine.medical_specialty ,China ,Article Subject ,Endocrinology, Diabetes and Metabolism ,Polymerase Chain Reaction ,Diseases of the endocrine glands. Clinical endocrinology ,chemistry.chemical_compound ,Endocrinology ,Western blot ,Fibrosis ,Polysaccharides ,Internal medicine ,medicine ,Oil Red O ,Animals ,Diabetic Nephropathies ,Kidney ,Triglyceride ,medicine.diagnostic_test ,Chemistry ,Cholesterol ,Anticoagulants ,Lipid metabolism ,medicine.disease ,Lipid Metabolism ,RC648-665 ,Rats ,Disease Models, Animal ,medicine.anatomical_structure ,Biomarkers ,Lipoprotein ,Research Article - Abstract
In this study, a diabetic kidney disease model was established by placing the test rats on a high-sugar/high-fat diet combined with streptozotocin induction. Histopathological examination (H&E, Masson, and PASM stain) showed pathological changes in the diabetic rat kidneys, in addition to fibrotic symptoms and collagen deposition. Immunohistochemistry and western blot analyses indicated that the diabetic condition significantly increased the expressions of fibrotic markers including collagen, α-SMA, and fibronectin. The levels of cholesterol, triglyceride, and low-density lipoprotein were also increased in diabetic kidney disease (DKD) rat blood, while the level of high-density lipoprotein was decreased. The results of Oil red O staining experiments indicated that the kidneys of diabetic rats exhibited appreciable fat deposition, with high contents of triglyceride and cholesterol. To inhibit fibrosis and reduce fat deposition, low molecular weight fucoidan (LMWF) may be used. Based on PCR and western blot analyses, LMWF can regulate the expression levels of important lipid metabolism regulators, thereby impeding the development of kidney fibrosis. Through the vitro model, it also be indicated that LMWF could inhibit fibrosis process through regulating lipid metabolism which induced by palmitic acid.
- Published
- 2021
- Full Text
- View/download PDF
25. Age-Dependent Increase of Bacterial Loads on Drosophila Surface Benefits the Flies to Combat Fungal Infections
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Yanlei Sun, Chengshu Wang, Song Hong, and Dapeng Sun
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biology ,media_common.quotation_subject ,fungi ,Age dependent ,Insect ,biology.organism_classification ,Spore ,Microbiology ,Immune system ,Microbiome ,Drosophila melanogaster ,Drosophila ,Function (biology) ,media_common - Abstract
In contrast to the well-recognized physiological importance of gut microbiomes, the composition and function of insect body-surface microbiota are still elusive and highly underexplored. Here we report the use of Drosophila melanogaster as a model for fungal infections and surface microbiome analysis. It was found that the older flies, even with a declined immune efficacy, survived better than the newly eclosed adults when being topically infected with fungal parasites. Our culture-dependent and independent analysis of fly surface microbiomes revealed the substantial increase of bacterial loads along with fly aging. Based on the detected bacterial species, it is suggestive that fly surface microbiotas were mainly assembled from fecal bacteria. In support of the fungal infection results, we found that the dominant bacterial species could effectively inhibit fungal spore germinations. Scanning microscope analysis revealed that fly tarsal segments were largely accumulated with bacterial cells, which could be feasibly dispersed onto different body parts by self-grooming to deter fungal spore germinations. These observations thus unveil a previously unsuspected strategy employed by flies to combat fungal infections in association with the age-dependent immune declines. The findings of this study not only shed lights on the feature and function of Drosophila external microbiomes but will also facilitate future investigations of the insect body-surface microbiotas effecting on insect physiologies.
- Published
- 2021
26. Gas-Fired Heat-Driven Duplex Stirling Domestic Combined Heat and Power System
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Yanlei Yanlei, Yanlei Sun, Jianying Hu, Ercang Luo, Kaiqi Luo, Zhanghua Wu, and Limin Zhang
- Published
- 2020
27. Upgrading Path and Value Chain Upgrading Strategy of China's Marine Equipment Manufacturing Industry
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Yanlei Sun and Sisi Dong
- Subjects
Sustainable development ,Ecology ,business.industry ,Chain (unit) ,Marine equipment ,Manufacturing ,Value (economics) ,China ,Marine industry ,business ,Industrial organization ,Earth-Surface Processes ,Water Science and Technology ,PATH (variable) - Abstract
Dong, S.-S., and Sun, Y.-L., 2020. Upgrading path and value chain upgrading strategy of China's marine equipment manufacturing industry. In: Qiu, Y.; Zhu, H., and Fang, X. (eds.), Current Advancements in Marine and Coastal Research for Technological and Sociological Applications. Journal of Coastal Research, Special Issue No. 107, pp. 157-160. Coconut Creek (Florida), ISSN 0749-0208.The upgrading of Marine Equipment Manufacturing Industry (MEMI) and value chain is of great value to the implementation of the strategy of building a strong marine country and the sustainable development of marine industry economy. Based on this, this paper first studies the upgrading mechanism of China's MEMI, then analyzes the upgrading principle and path of MEMI and value chain, and finally gives the upgrading path and value chain construction strategy of MEMI.
- Published
- 2020
28. [Corrigendum] MicroRNA-137 has a suppressive role in liver cancer via targeting EZH2
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Jinbao Wang, Chengbiao Liu, Shichang Cui, Guang Hao, Yanlei Sun, Qidong Sun, and Yang Liu
- Subjects
0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,business.industry ,Biochemistry ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,030220 oncology & carcinogenesis ,Family medicine ,Genetics ,Molecular Medicine ,Medicine ,business ,Molecular Biology - Abstract
Following the publication of this article, we realize that an error was made with the second author's (Yanlei Sun's) address: This should have been written as "Department of General Surgery, Cancer Hospital of Linyi, Linyi, Shandong 276001", not as "Department of General Surgery, Central Hospital of Linyi, Linyi, Shandong 276400". Therefore, the author affiliations and addresses in this paper should have appeared as follows: SHICHANG CUI1, YANLEI SUN2, YANG LIU3, CHENGBIAO LIU1, JINBAO WANG1, GUANG HAO1 and QIDONG SUN1 1Department of General Surgery, Central Hospital of Linyi, Linyi, Shandong 276400; 2Department of General Surgery, Cancer Hospital of Linyi, Linyi, Shandong 276001; 3Department of Obstetrics and Gynecology, Central Hospital of Linyi, Linyi, Shandong 276400, P.R. China. The authors regret this error in the affiliation, and apologize for any inconvenience caused. [the original article was published in the Molecular Medicine Reports 16: 9494-9502, 2017; DOI: 10.3892/mmr.2017.7828].
- Published
- 2018
29. P2RX7 functions as a putative biomarker of gastric cancer and contributes to worse prognosis
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Lili, Wang, primary, Yun, Li, additional, Tingran, Wei, additional, Xia, Wu, additional, and Yanlei, Sun, additional
- Published
- 2019
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30. ElastoHydroDynamic lubricated cylindrical joints for rigid-flexible multibody dynamics
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Cheng Liu, Qiang Tian, Yanlei Sun, Haiyan Hu, Paulo Flores, and Universidade do Minho
- Subjects
Absolute Nodal Coordinate Formulation (ANCF) ,Flexibility (anatomy) ,Materials science ,02 engineering and technology ,Elastohydrodynamic (EHD) ,01 natural sciences ,7. Clean energy ,law.invention ,Physics::Fluid Dynamics ,0203 mechanical engineering ,law ,0103 physical sciences ,medicine ,Multibody dynamics ,General Materials Science ,Lubricant ,Composite material ,010301 acoustics ,Civil and Structural Engineering ,Science & Technology ,Bearing (mechanical) ,biology ,Mechanical Engineering ,Finite difference method ,Cylindrical joint ,Mechanics ,Multibody system ,biology.organism_classification ,Natural Coordinate Formulation (NCF) ,Computer Science Applications ,020303 mechanical engineering & transports ,medicine.anatomical_structure ,Modeling and Simulation ,Lubricated cylindrical joint ,Hexahedron ,Adina - Abstract
A new methodology for dynamic analysis of rigid-flexible multibody systems with elastohydrodynamic (EHD) lubricated cylindrical joints is proposed. The EHD lubricated cylindrical joint is formulated by the Natural Coordinate Formulation (NCF) and the twenty- node hexahedral element of Absolute Nodal Coordinate Formulation (ANCF), being the lubricant pressure determined through the resolution of the Reynolds’ equation employing the finite difference method. The main outcomes are validated with those obtained by using the commercial software ADINA. It is shown that the bearing flexibility plays a significant role in the system responses, extends the lubricant distribution space and, consequently, reduces the lubricant pressure., Fundação para a Ciência e a Tecnologia (FCT)
- Published
- 2013
31. Combined effects of FTO rs9939609 and MC4R rs17782313 on obesity and BMI in Chinese Han populations
- Author
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Jiazhong Sun, Wei Huang, and Yanlei Sun
- Subjects
Male ,China ,medicine.medical_specialty ,Genotype ,endocrine system diseases ,Endocrinology, Diabetes and Metabolism ,Alpha-Ketoglutarate-Dependent Dioxygenase FTO ,Type 2 diabetes ,Body Mass Index ,Endocrinology ,Gene Frequency ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Obesity ,Chinese han ,Allele ,Aged ,Genetics ,Polymorphism, Genetic ,business.industry ,Proteins ,nutritional and metabolic diseases ,Middle Aged ,medicine.disease ,Diabetes Mellitus, Type 2 ,Risk allele ,Receptor, Melanocortin, Type 4 ,Female ,Gene polymorphism ,business - Abstract
Genetic variants of FTO and MC4R have been linked with obesity and T2DM in populations of Europeans. In this study, we have investigated the association of FTO rs9939609 and MC4R rs17782313 with obesity and T2DM in the Chinese population and analyzed the relationship between rs9939609 and rs17782313. 2351 individuals were recruited. We tested the rs9939609 and rs17782313 by sequences retrieval method. Clinical and biochemical characteristics were measured. The rs9939609 per-A allele and rs17782313 per-C allele increases of OR for obesity was 1.42 (95% CI 1.39–3.74) and 1.39 (95% CI 1.21–3.53).The genotypic OR for obesity was 1.92 (95% CI 1.81–4.67) for AA genotype, 1.71 (95% CI 1.47–4.54) for AT genotype, 1.87 (95% CI 1.72–4.00) for CC genotype, and 1.44 (95% CI 1.20–3.18) for CT genotype. BMI of participants carrying neither FTO nor MC4R risk allele was 25.9 ± 4.9, one risk allele was 26.4 ± 5.1, two risk alleles was 28.1 ± 5.5, and there or four risk alleles was 33.2 ± 6.3. We found no association between FTO and MC4R and the Chinese population with T2DM (P > 0.05). Our data support that the rs9939609 and rs17782313 are strongly associated with obesity and BMI. Their combined effects were significant in Chinese population. No association between FTO and MC4R and Chinese population with T2DM was found.
- Published
- 2010
32. Intermittent high glucose enhances cell proliferation and VEGF expression in retinal endothelial cells: the role of mitochondrial reactive oxygen species
- Author
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Suxin Sun, Yancheng Xu, Xiang Wang, Yanlei Sun, and Jiazhong Sun
- Subjects
Vascular Endothelial Growth Factor A ,Mitochondrial ROS ,medicine.medical_specialty ,Clinical Biochemistry ,Enzyme-Linked Immunosorbent Assay ,Biology ,medicine.disease_cause ,Retina ,chemistry.chemical_compound ,Internal medicine ,medicine ,Humans ,Endothelium ,Molecular Biology ,Cells, Cultured ,Mitochondrial transport ,Cell Proliferation ,chemistry.chemical_classification ,Reactive oxygen species ,Dose-Response Relationship, Drug ,Cell growth ,Nitrotyrosine ,Deoxyguanosine ,Cell Biology ,General Medicine ,Blotting, Northern ,Vascular endothelial growth factor ,Oxidative Stress ,Glucose ,Endocrinology ,chemistry ,L-Glucose ,8-Hydroxy-2'-Deoxyguanosine ,Tyrosine ,Reactive Oxygen Species ,Oxidative stress - Abstract
Proliferation of human retinal endothelial cells (HRECs) is an important event in the development of diabetic retinopathy. Glucose fluctuations are strong predictor of diabetic vascular complications. In this study we have investigated the effect of intermittent high glucose on proliferation and expression of vascular endothelial growth factor (VEGF) in HRECs. The possible involvement of mitochondrial reactive oxygen species (ROS) was assessed. HRECs were incubated for 72 h in media containing different glucose concentrations: 5, 25, 5 mmol/l alternating with 25 mmol/l glucose, with or without Mn(III)tetrakis(4-benzoic acid) porphyrin chloride (MnTBAP) and thenoyltri-fluoroacetone (TTFA). The cell proliferation, VEGF expression, mitochondrial ROS, nitrotyrosine and 8-hydroxydeoxyguanosine (8-OHdG) were measured. In cultured HRECs, treatment with constant or intermittent high glucose significantly increased [(3)H]thymidine incorporation in a time-dependent manner. Treatment with constant high glucose for 48 h resulted in significant increases in [(3)H]thymidine incorporation, mRNA and protein levels of VEGF compared with HRECs treated with the normal glucose, which were markedly enhanced in cells exposed to intermittent high glucose. The levels of mitochondrial ROS, nitrotyrosine and 8-OhdG were significantly elevated under both intermittent and constant high glucose conditions, the effect being greater under intermittent high glucose. In addition, the antioxidants MnTBAP or TTFA can effectively prevent cell proliferation and overexpression of VEGF, as well as overproduction of mitochondrial ROS, nitrotyrosine and 8-OhdG in HRECs induced by constant or intermittent high glucose. Intermittent high glucose enhances cell proliferation and overexpression of VEGF through reactive oxygen species (ROS) overproduction at the mitochondrial transport chain level in HRECs, indicating that glycemic variability have important pathological effects on the development of diabetic retinopathy dependent of mitochondrial ROS.
- Published
- 2010
33. MicroRNA-137 has a suppressive role in liver cancer via targeting EZH2
- Author
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Guang Hao, Chengbiao Liu, Jinbao Wang, Yanlei Sun, Yang Liu, Qidong Sun, and Shichang Cui
- Subjects
0301 basic medicine ,Adult ,Male ,Cancer Research ,Carcinoma, Hepatocellular ,proliferation ,Biology ,medicine.disease_cause ,migration ,Biochemistry ,Metastasis ,enhancer of zeste homologue 2 ,liver cancer ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,microRNA ,Genetics ,medicine ,Humans ,Enhancer of Zeste Homolog 2 Protein ,Neoplasm Invasiveness ,Molecular Biology ,Aged ,Cell Proliferation ,Oncogene ,Cell growth ,Liver Neoplasms ,Cancer ,Hep G2 Cells ,Articles ,hepatocellular carcinoma ,Cell cycle ,Middle Aged ,medicine.disease ,invasion ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Molecular Medicine ,Female ,Liver cancer ,Carcinogenesis ,Corrigendum - Abstract
A variety of microRNAs (miRs) have been demonstrated to be associated with the development and malignant progression of human cancer; however, the regulatory mechanism of miR‑137 underlying hepatocellular carcinoma (HCC) growth and metastasis still remains to be fully revealed. In the present study, reverse transcription‑quantitative polymerase chain reaction and western blot were used to examine mRNA and protein expression. MTT assay, wound healing assay and Transwell assay were performed to determine cell proliferation, migration and invasion. Luciferase reporter assay was conducted to confirm the targeting relationship. miR‑137 was significantly downregulated in HCC tissues compared to adjacent normal tissues. Low expression of miR‑137 was significantly associated with lymph node metastasis, vein invasion, advanced clinical stage and poor prognosis in HCC. In addition, miR‑137 was also downregulated in several liver cancer cell lines compared with normal liver epithelial cells. Overexpression of miR‑137 led to a significant reduction in cell proliferation, migration and invasion of HepG2 cells. Enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) was further identified as a direct target gene of miR‑137, and the protein expression of EZH2 was negatively regulated by miR‑137 in HepG2 cells. Additionally, EZH2 was significantly upregulated in HCC tissues and liver cancer cell lines. Furthermore, overexpression of EZH2 significantly eliminated the inhibitory effects of miR‑137 on the malignant phenotypes of HepG2 cells. Therefore, the findings suggest that miR‑137 may have a suppressive role in HCC growth and metastasis via targeting EZH2.
- Published
- 2018
34. Intermittent high glucose enhances proliferation of vascular smooth muscle cells by upregulating osteopontin
- Author
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Yancheng Xu, Yanlei Sun, Zhe Dai, and Jiazhong Sun
- Subjects
Male ,medicine.medical_specialty ,Vascular smooth muscle ,Myocytes, Smooth Muscle ,Integrin ,Stimulation ,Biology ,Matrix metalloproteinase ,Biochemistry ,Muscle, Smooth, Vascular ,chemistry.chemical_compound ,Endocrinology ,Internal medicine ,medicine ,Animals ,Humans ,Osteopontin ,Rats, Wistar ,Receptor ,Molecular Biology ,Cells, Cultured ,Cell Proliferation ,Cell growth ,Rats ,Glucose ,L-Glucose ,chemistry ,Hyperglycemia ,biology.protein ,Matrix Metalloproteinase 2 - Abstract
Objective Hyperglycemia induces vascular smooth muscle cells (VSMCs) proliferation and may thus contribute to the formation of atherosclerotic lesions. Glucose fluctuations are strong predictor of diabetic vascular complications. We investigate the effects of exposure to constant and intermittent high glucose concentrations on the proliferation and matrix metalloproteinase (MMP)-2 activity of rat aortic VSMCs in culture, as well as the expression of osteopontin (OPN). Methods Rat aortic VSMCs were grown to confluence and then exposed to 5 mmol/L glucose, 25 mmol/L glucose, or 5 mmol/L alternating with 25 mmol/L glucose in the absence or presence of neutralizing antibodies to OPN, β3 integrin receptor and β5 integrin receptor. The cell proliferation, MMP-2 activity and the expression of OPN were assessed. Results In cultured VSMCs, treatment with constant or intermittent high glucose significantly increased [3H]thymidine incorporation in a time-dependent manner. A modest increase was observed at 12 h, and further deteriorated afterwards, and reached the maximum expression at 48 h. However, [3H]thymidine incorporation was more pronounced in intermittent high glucose than in constant high glucose. Treatment with constant high glucose for 48 h significantly increase cell number, MMP-2 activation, OPN protein and mRNA expression compared with VSMCs treated with the cells normal glucose, and these effects were further enhanced when VSMCs were treated with intermittent high glucose. In addition, neutralizing antibodies to either OPN or its receptor β3 integrin but not neutralizing antibodies to β5 integrin significantly suppressed increase in [3H]thymidine incorporation and MMP-2 activity induced by constant or intermittent high glucose. Conclusions In cultured VSMCs, constant high glucose concentrations enhanced MMP-2 activity, cell proliferation and OPN expression. These effects are enhanced following intermittent exposure to high glucose, indicating that short lived excursions in glycaemic control have important pathological effects on the development of diabetic atherosclerosis, which is mediated by the stimulation of OPN expression and synthesis.
- Published
- 2009
35. Intermittent High Glucose Stimulate MCP-l, IL-18, and PAI-1, but Inhibit Adiponectin Expression and Secretion in Adipocytes Dependent of ROS
- Author
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Yancheng Xu, Zhe Dai, Jiazhong Sun, and Yanlei Sun
- Subjects
medicine.medical_specialty ,Biophysics ,Adipokine ,Biology ,Biochemistry ,Mice ,chemistry.chemical_compound ,3T3-L1 Cells ,Internal medicine ,Plasminogen Activator Inhibitor 1 ,Adipocytes ,medicine ,Animals ,Secretion ,Chemokine CCL2 ,chemistry.chemical_classification ,Reactive oxygen species ,Adiponectin ,Monocyte ,Interleukin-18 ,Deoxyguanosine ,Cell Biology ,General Medicine ,Glucose ,Endocrinology ,medicine.anatomical_structure ,L-Glucose ,chemistry ,8-Hydroxy-2'-Deoxyguanosine ,Plasminogen activator inhibitor-1 ,Reactive Oxygen Species ,Plasminogen activator - Abstract
Elevated circulating concentrations of interleukin-18 (IL-18), monocyte chemoattractant protein-1 (MCP-1), and plasminogen activator inhibitor-1 (PAI-1) and decrease of adiponectin are associated with obesity-related diseases. The mechanism that mediates the aberrant production of the adipokines remains poorly understood. The aim of this study was to investigate the effect of intermittent high glucose on the expression of IL-18, MCP-1, and PAI-1 and adiponectin in 3T3-L1 adipocytes. 3T3-L1 adipocytes were incubated for 24 h in media containing different glucose concentrations: 5 mmol/l, 20 mmol/l and a daily alternating 5 or 20 mmol/l glucose, with or without the addition of 1.0 mmol/l N-acetylcysteine (NAC). The expression and secretion of IL-18, MCP-1, PAI-1, and adiponectin were determined by real-time RT-PCR and ELISA, respectively. The production of reactive oxygen species (ROS) and 8-hydroxydeoxyguanosine (8-OHdG) were measured. Stable high glucose significantly increased expression and secretion of IL-18, MCP-1, and PAI-1, and reduced adiponectin expression and secretion compared to normal glucose conditions. These effects were significantly greater under intermittent high glucose conditions compared to stable high glucose. The level of ROS and 8-OHdG were significantly elevated under both intermittent and stable high glucose conditions, the effect being greater under intermittent high glucose. The intermittent glucose was more effective in triggering the generation of ROS than stable high glucose. The adding of the NAC, a specific pharmacological inhibitor of ROS, normalized the expression of these adipokines and the levels of ROS and 8-OHdG under both stable and intermittent glucose conditions. Intermittent high glucose induces a greater aberrant production of key adipokines than stable high glucose, and this effect seems to be related to over-production of ROS.
- Published
- 2009
36. Coupling dynamics of a geared multibody system supported by Elastohydrodynamic lubricated cylindrical joints
- Author
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Qianfei Xiao, Paulo Flores, Qiang Tian, Yanlei Sun, Haiyan Hu, Hui Liu, and Universidade do Minho
- Subjects
Absolute Nodal Coordinate Formulation (ANCF) ,Control and Optimization ,ElastoHydroDynamic (EHD) lubrication ,Engenharia e Tecnologia::Engenharia Mecânica ,Aerospace Engineering ,law.invention ,Contact force ,Control theory ,law ,medicine ,Coupling ,Physics ,Bearing (mechanical) ,Science & Technology ,business.industry ,4. Education ,Mechanical Engineering ,Finite difference method ,Stiffness ,Engenharia Mecânica [Engenharia e Tecnologia] ,Structural engineering ,Multibody system ,Physics::Classical Physics ,Computer Science Applications ,Line of action ,Modeling and Simulation ,Time-varying meshing stiffness ,Lubrication ,Sliding frictional force ,medicine.symptom ,business ,Geared multibody system - Abstract
A comprehensive computational methodology to study the coupling dynamics of a geared multibody system supported by ElastoHydroDynamic (EHD) lubricated cylindrical joints is proposed throughout this work. The geared multibody system is described by using the Absolute-Coordinate-Based (ACB) method that combines the Natural Coordinate Formulation (NCF) describing rigid bodies and the Absolute Nodal Coordinate Formulation (ANCF) characterizing the flexible bodies. Based on the finite-short bearing approach, the EHD lubrication condition for the cylindrical joints supporting the geared system is considered here. The lubrication forces developed at the cylindrical joints are obtained by solving the Reynolds’ equation via the finite difference method. For the evaluation of the normal contact forces of gear pair along the Line Of Action (LOA), the time-varying mesh stiffness, mesh damping and Static Transmission Error (STE) are utilized. The time-varying mesh stiffness is calculated by using the Chaari’s methodology. The forces of sliding friction along the Off-Line-Of-Action (OLOA) are computed by using the Coulomb friction models with a time-varying coefficient of friction under the EHD lubrication condition of gear teeth. Finally, two numerical examples of application are presented to demonstrate and validate the proposed methodology., National Natural Science Foundations of China under Grant 11290151, 11221202 and 11002022, Beijing Higher Education Young Elite Teacher Project under Grant YETP1201.
- Published
- 2015
37. Highlight Article: P2RX7 functions as a putative biomarker of gastric cancer and contributes to worse prognosis.
- Author
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Lili, Wang, Yun, Li, Tingran, Wei, Xia, Wu, and Yanlei, Sun
- Published
- 2019
- Full Text
- View/download PDF
38. Combined effects of FTO rs9939609 and MC4R rs17782313 on elevated nocturnal blood pressure in the Chinese Han population
- Author
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Yanlei Sun, Jiazhong Sun, Mei Yang, and Jun Wu
- Subjects
Adult ,medicine.medical_specialty ,gene polymorphism ,Alpha-Ketoglutarate-Dependent Dioxygenase FTO ,030209 endocrinology & metabolism ,Blood Pressure ,030204 cardiovascular system & hematology ,Nocturnal ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Gene Frequency ,Polymorphism (computer science) ,Risk Factors ,Internal medicine ,medicine ,Odds Ratio ,Humans ,Genetic Predisposition to Disease ,Obesity ,Allele ,Allele frequency ,Aged ,business.industry ,Cardiovascular Topics ,nutritional and metabolic diseases ,General Medicine ,Odds ratio ,Middle Aged ,medicine.disease ,daytime blood pressure ,Chinese Han population ,nocturnal blood pressure ,Blood pressure ,Endocrinology ,Hypertension ,Receptor, Melanocortin, Type 4 ,Cardiology and Cardiovascular Medicine ,business - Abstract
Summary Aim In this study we investigated the association of FTO rs9939609 and MC4R. rs17782313 with elevated blood pressure in the Chinese Han population, and analysed the relationship between the rs9939609 and rs17782313 variants. Methods We tested the rs9939609 and rs17782313 variants with the sequence-retrieval method. Results The increase in odds ratios of the A allele of rs9939609 and the C allele of rs17782313 for nocturnal blood pressure were 1.37 and 1.69. The nocturnal blood pressure of participants simultaneously carrying the A and C alleles was significantly higher than the blood pressure of those carrying neither FTO nor MC4R risk alleles (p < 0.05), and that of the controls carrying only the A or C alleles (p < 0.05). No association between the FTO or MC4R genes with daytime hypertension was found in this Chinese population (p > 0.05). Conclusion Our data suggest that the rs9939609 and rs17782313 variants may be significantly associated with nocturnal but not daytime blood pressure levels and their combined effects were significant in this Chinese Han population.
- Published
- 2014
39. MicroRNA-137 has a suppressive role in liver cancer via targeting EZH2.
- Author
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SHICHANG CUI, YANLEI SUN, YANG LIU, CHENGBIAO LIU, JINBAO WANG, GUANG HAO, and QIDONG SUN
- Subjects
- *
MICRORNA , *LIVER cancer , *CANCER invasiveness , *REGULATOR genes , *GENETIC regulation , *PROTEIN expression , *GENETICS - Abstract
A variety of microRNAs (miRs) have been demonstrated to be associated with the development and malignant progression of human cancer; however, the regulatory mechanism of miR-137 underlying hepatocellular carcinoma (HCC) growth and metastasis still remains to be fully revealed. In the present study, reverse transcription-quantitative polymerase chain reaction and western blot were used to examine mRNA and protein expression. MTT assay, wound healing assay and Transwell assay were performed to determine cell proliferation, migration and invasion. Luciferase reporter assay was conducted to confirm the targeting relationship. miR-137 was significantly downregulated in HCC tissues compared to adjacent normal tissues. Low expression of miR-137 was significantly associated with lymph node metastasis, vein invasion, advanced clinical stage and poor prognosis in HCC. In addition, miR-137 was also downregulated in several liver cancer cell lines compared with normal liver epithelial cells. Overexpression of miR-137 led to a significant reduction in cell proliferation, migration and invasion of HepG2 cells. Enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) was further identified as a direct target gene of miR-137, and the protein expression of EZH2 was negatively regulated by miR-137 in HepG2 cells. Additionally, EZH2 was significantly upregulated in HCC tissues and liver cancer cell lines. Furthermore, overexpression of EZH2 significantly eliminated the inhibitory effects of miR-137 on the malignant phenotypes of HepG2 cells. Therefore, the findings suggest that miR-137 may have a suppressive role in HCC growth and metastasis via targeting EZH2. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
40. Intermittent high glucose exacerbates the aberrant production of adiponectin and resistin through mitochondrial superoxide overproduction in adipocytes
- Author
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Haohua Deng, Suxin Sun, Yanlei Sun, Zhe Dai, Jiazhong Sun, and Yancheng Xu
- Subjects
medicine.medical_specialty ,Metalloporphyrins ,Adipokine ,Enzyme-Linked Immunosorbent Assay ,Biology ,medicine.disease_cause ,Antioxidants ,chemistry.chemical_compound ,Mice ,Endocrinology ,Superoxides ,Internal medicine ,3T3-L1 Cells ,medicine ,Adipocytes ,Animals ,Resistin ,Molecular Biology ,Mitochondrial transport ,Thenoyltrifluoroacetone ,chemistry.chemical_classification ,Reactive oxygen species ,Adiponectin ,Superoxide ,Nitrotyrosine ,Deoxyguanosine ,Mitochondria ,Glucose ,chemistry ,8-Hydroxy-2'-Deoxyguanosine ,Tyrosine ,Oxidative stress - Abstract
Hypoadiponectinemia and hyperresistinemia may be important in mediating signals from adipocytes to insulin-sensitive tissue and vasculature. However, the mechanism that mediates the aberrant production of adipokines remains poorly understood. In this study, we have investigated the effect of intermittent high glucose on the expression of adiponectin and resistin, and the production of 8-hydroxydeoxyguanosine (8-OHdG) and nitrotyrosine in the adipocytes, either in the presence or in the absence of Mn(III) tetrakis(4-benzoic acid) porphyrin chloride (MnTBAP) or thenoyltrifluoroacetone (TTFA). 3T3-L1 adipocytes were incubated for 72 h in media containing different glucose concentrations: 5 mmol/l, 20 mmol/l, 5 mmol/l alternating with 20 mmol/l glucose, with or without MnTBAP and TTFA. We measured the expression of resistin and adiponectin. The production of nitrotyrosine and 8-OHdG as oxidative stress parameter was measured. Both constant and intermittent high glucose significantly suppressed the expression and secretion of adiponectin, and increased expression and secretion of resistin in mature adipocytes compared to normal glucose conditions. However, these effects were significantly greater under intermittent high glucose conditions compared to constant high glucose. The levels of nitrotyrosine and 8-OHdG were significantly elevated under both intermittent and constant high glucose conditions, the effect being greater under intermittent high glucose. In addition, the antioxidants MnTBAP or TTFA reversed the aberrant production of adiponectin and resistin, as well as overproduction of nitrotyrosine and 8-OHdG in adipocytes induced by constant or intermittent high glucose. Intermittent high glucose exacerbates the aberrant production of adiponectin and resistin through reactive oxygen species overproduction at the mitochondrial transport chain level in adipocytes, indicating that glycemic variability has important pathological effects on the secretion of adipokines.
- Published
- 2010
41. Involvement of osteopontin upregulation on mesangial cells growth and collagen synthesis induced by intermittent high glucose
- Author
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Yanlei Sun, Haohua Deng, Zhe Dai, Suxin Sun, Jiazhong Sun, and Yancheng Xu
- Subjects
Male ,medicine.medical_specialty ,Cell Survival ,Integrin ,Blotting, Western ,Stimulation ,Biochemistry ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Type IV collagen ,stomatognathic system ,Downregulation and upregulation ,Internal medicine ,medicine ,Animals ,Osteopontin ,Receptor ,Molecular Biology ,Cells, Cultured ,Cell Proliferation ,biology ,Cell growth ,Cell Biology ,Blotting, Northern ,Rats ,Endocrinology ,Glucose ,L-Glucose ,chemistry ,Mesangial Cells ,biology.protein ,Collagen - Abstract
Glucose fluctuations are strong predictor of diabetic vascular complications. We explored the effects of constant and intermittent high glucose on the proliferation and collagen synthesis of cultured rat mesangial cells. Furthermore, the possible involvement of osteopontin (OPN) was assessed. In rat mesangial cells cultured in 5, 25, or 5 mmol/L alternating with 25 mmol/L glucose in the absence or presence of neutralizing antibodies to OPN, beta3 integrin receptor and beta5 integrin receptor, the cell proliferation, collagen synthesis, and the expression of OPN and type IV collagen were assessed. In cultured mesangial cells, treatment with constant or intermittent high glucose significantly increased [(3)H]thymidine incorporation in a time-dependent manner. A modest increase was observed at 12 h, and further deteriorated afterwards, and reached the maximum incorporation at 48 h. Treatment with constant high glucose for 48 h resulted in significant increases in [(3)H]thymidine incorporation, cell number, [(3)H]proline incorporation, mRNA, and protein levels of type IV collagen and OPN compared with mesangial cells treated with the normal glucose, which were markedly enhanced in cells exposed to intermittent high glucose medium. In addition, neutralizing antibodies to either OPN or its receptor beta3 integrin but not neutralizing antibodies to beta5 integrin can effectively prevented proliferation and collagen synthesis of mesangial cells induced by constant or intermittent high glucose. Intermittent high glucose exacerbates mesangial cells growth and collagen synthesis by upregulation of OPN expression, indicating that glycemic variability have important pathological effects on the development of diabetic nephropathy, which is mediated by the stimulation of OPN expression and synthesis.
- Published
- 2010
42. Combined effects of FTO rs9939609 and MC4R rs17782313 on elevated nocturnal blood pressure in the Chinese Han population.
- Author
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Yanlei Sun, Jiazhong Sun, Jun Wu, and Mei Yang
- Published
- 2016
- Full Text
- View/download PDF
43. Erratum: Combined effects of FTO rs9939609 and MC4R rs17782313 on obesity and BMI in Chinese Han populations
- Author
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Wei Huang, Yanlei Sun, and Jiazhong Sun
- Subjects
Endocrinology ,Endocrinology, Diabetes and Metabolism - Published
- 2010
44. Variants in the fat mass and obesity associated (FTO) gene are associated with obesity and C-reactive protein levels in Chinese Han populations
- Author
-
Jiazhong Sun, Mei Yang, Xiang Wang, Wei You, and Yanlei Sun
- Subjects
Male ,medicine.medical_specialty ,Waist ,Genotype ,Alpha-Ketoglutarate-Dependent Dioxygenase FTO ,FTO gene ,Asian People ,Polymorphism (computer science) ,Internal medicine ,medicine ,Humans ,Genetic Predisposition to Disease ,Obesity ,Aged ,Polymorphism, Genetic ,biology ,business.industry ,C-reactive protein ,Proteins ,nutritional and metabolic diseases ,General Medicine ,Odds ratio ,Middle Aged ,medicine.disease ,C-Reactive Protein ,Endocrinology ,biology.protein ,Female ,business - Abstract
Purpose: Various genetic variants of the fat mass and obesity associated gene (FTO) have been linked to obesity in populations of Europeans. Low-grade inflammation is a key feature of obesity, characterized by elevated plasma levels of C-reactive protein (CRP). In the present study, we have investigated whether the FTO-risk variant is associated with obesity and CRP in the Chinese population. Methods: 1799 individuals aged 50-70 years (896 men and 903 women), including both obese (560 cases) and control individuals, (1200 cases) were recruited. The FTO rs9939609 polymorphism was tested using sequences retrieval. Waist circumference, body fat, hs-CRP, blood total cholesterol, triglycerides and LDL-C were also measured. Results: The FTO rs9939609 polymorphism per-A allele was associated with an increased odds ratio for obesity of 1.42 (95%CI 1.23-1.64). The genotypic odds ratio for obesity was 1.92 (95%CI 1.43-2.57) for the AA genotype, 1.71 (95%CI 1.35-2.16) for the AT genotype. The association between FTO rs9939609 polymorphism and CRP levels was r=0.78. Conclusion: Our data support the hypothesis that FTO rs9939609 polymorphism is strongly associated with obesity and CRP levels in the Chinese population as reported in the European population.
- Published
- 2010
45. Combined effects of FTO rs9939609 and MC4R rs17782313 on obesity and BMI in Chinese Han populations.
- Author
-
Wei Huang, Yanlei Sun, and Jiazhong Sun
- Abstract
Genetic variants of FTO and MC4R have been linked with obesity and T2DM in populations of Europeans. In this study, we have investigated the association of FTO rs9939609 and MC4R rs17782313 with obesity and T2DM in the Chinese population and analyzed the relationship between rs9939609 and rs17782313. 2351 individuals were recruited. We tested the rs9939609 and rs17782313 by sequences retrieval method. Clinical and biochemical characteristics were measured. The rs9939609 per-A allele and rs17782313 per-C allele increases of OR for obesity was 1.42 (95% CI 1.39-3.74) and 1.39 (95% CI 1.21-3.53).The genotypic OR for obesity was 1.92 (95% CI 1.81-4.67) for AA genotype, 1.71 (95% CI 1.47-4.54) for AT genotype, 1.87 (95% CI 1.72-4.00) for CC genotype, and 1.44 (95% CI 1.20-3.18) for CT genotype. BMI of participants carrying neither FTO nor MC4R risk allele was 25.9 ± 4.9, one risk allele was 26.4 ± 5.1, two risk alleles was 28.1 ± 5.5, and there or four risk alleles was 33.2 ± 6.3. We found no association between FTO and MC4R and the Chinese population with T2DM ( P > 0.05). Our data support that the rs9939609 and rs17782313 are strongly associated with obesity and BMI. Their combined effects were significant in Chinese population. No association between FTO and MC4R and Chinese population with T2DM was found. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
46. Variants in the fat mass and obesity associated (FTO) gene are associated with obesity and C-reactive protein levels in Chinese Han populations.
- Author
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Yanlei Sun, Jiazhong Sun, Xiang Wang, Wei You, and Mei Yang
- Subjects
- *
OBESITY , *GENETIC polymorphisms , *C-reactive protein , *TRIGLYCERIDES , *BLOOD cholesterol ,HEALTH of Chinese people - Abstract
Purpose: Various genetic variants of the fat mass and obesity associated gene (FTO) have been linked to obesity in populations of Europeans. Low- grade inflammation is a key feature of obesity, characterized by elevated plasma levels of C-reactive protein (CRP). In the present study, we have investigated whether the FTO-risk variant is associated with obesity and CRP in the Chinese population. Methods: 1799 individuals aged 50-70 years (896 men and 903 women), including both obese (560 cases) and control individuals, (1200 cases) were recruited. "the FTO rs9939609 polymorphism was tested using sequences retrieval. Waist circumference, body fat, hs-CRP, blood total cholesterol, triglycerides and LDL-C were also measured. Results: "the FTO rs9939609 polymorphism per-A allele was associated with an increased odds ratio for obesity of 1.42 (95%CI 1.23-1.64). "the genotypic odds ratio for obesity was 1.92 (95%CI 1.43-2.57) for the AA genotype, 1.71 (95%CI 1.35-2.16) for the AT genotype. "the association between FTO rs9939609 polymorphism and CRP levels was r=0.78. Conclusion: Our data support the hypothesis that FTO rs9939609 polymorphism is strongly associated with obesity and CRP levels in the Chinese population as reported in the European population. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
47. Intermittent high glucose enhances cell proliferation and VEGF expression in retinal endothelial cells: the role of mitochondrial reactive oxygen species.
- Author
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Jiazhong Sun, Yancheng Xu, Suxin Sun, Yanlei Sun, and Xiang Wang
- Abstract
Proliferation of human retinal endothelial cells (HRECs) is an important event in the development of diabetic retinopathy. Glucose fluctuations are strong predictor of diabetic vascular complications. In this study we have investigated the effect of intermittent high glucose on proliferation and expression of vascular endothelial growth factor (VEGF) in HRECs. The possible involvement of mitochondrial reactive oxygen species (ROS) was assessed. HRECs were incubated for 72 h in media containing different glucose concentrations: 5, 25, 5 mmol/l alternating with 25 mmol/l glucose, with or without Mn(III)tetrakis(4-benzoic acid) porphyrin chloride (MnTBAP) and thenoyltri-fluoroacetone (TTFA). The cell proliferation, VEGF expression, mitochondrial ROS, nitrotyrosine and 8-hydroxydeoxyguanosine (8-OHdG) were measured. In cultured HRECs, treatment with constant or intermittent high glucose significantly increased [H]thymidine incorporation in a time-dependent manner. Treatment with constant high glucose for 48 h resulted in significant increases in [H]thymidine incorporation, mRNA and protein levels of VEGF compared with HRECs treated with the normal glucose, which were markedly enhanced in cells exposed to intermittent high glucose. The levels of mitochondrial ROS, nitrotyrosine and 8-OhdG were significantly elevated under both intermittent and constant high glucose conditions, the effect being greater under intermittent high glucose. In addition, the antioxidants MnTBAP or TTFA can effectively prevent cell proliferation and overexpression of VEGF, as well as overproduction of mitochondrial ROS, nitrotyrosine and 8-OhdG in HRECs induced by constant or intermittent high glucose. Intermittent high glucose enhances cell proliferation and overexpression of VEGF through reactive oxygen species (ROS) overproduction at the mitochondrial transport chain level in HRECs, indicating that glycemic variability have important pathological effects on the development of diabetic retinopathy dependent of mitochondrial ROS. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
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48. Intermittent High Glucose Stimulate MCP-l, IL-18, and PAI-1, but Inhibit Adiponectin Expression and Secretion in Adipocytes Dependent of ROS.
- Author
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Jiazhong Sun, Yancheng Xu, Zhe Dai, and Yanlei Sun
- Abstract
Abstract Elevated circulating concentrations of interleukin-18 (IL-18), monocyte chemoattractant protein-1 (MCP-1), and plasminogen activator inhibitor-1 (PAI-1) and decrease of adiponectin are associated with obesity-related diseases. The mechanism that mediates the aberrant production of the adipokines remains poorly understood. The aim of this study was to investigate the effect of intermittent high glucose on the expression of IL-18, MCP-1, and PAI-1 and adiponectin in 3T3-L1 adipocytes. 3T3-L1 adipocytes were incubated for 24 h in media containing different glucose concentrations: 5 mmol/l, 20 mmol/l and a daily alternating 5 or 20 mmol/l glucose, with or without the addition of 1.0 mmol/l N-acetylcysteine (NAC). The expression and secretion of IL-18, MCP-1, PAI-1, and adiponectin were determined by real-time RT-PCR and ELISA, respectively. The production of reactive oxygen species (ROS) and 8-hydroxydeoxyguanosine (8-OHdG) were measured. Stable high glucose significantly increased expression and secretion of IL-18, MCP-1, and PAI-1, and reduced adiponectin expression and secretion compared to normal glucose conditions. These effects were significantly greater under intermittent high glucose conditions compared to stable high glucose. The level of ROS and 8-OHdG were significantly elevated under both intermittent and stable high glucose conditions, the effect being greater under intermittent high glucose. The intermittent glucose was more effective in triggering the generation of ROS than stable high glucose. The adding of the NAC, a specific pharmacological inhibitor of ROS, normalized the expression of these adipokines and the levels of ROS and 8-OHdG under both stable and intermittent glucose conditions. Intermittent high glucose induces a greater aberrant production of key adipokines than stable high glucose, and this effect seems to be related to over-production of ROS. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
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