1. RAB27A, RAB27B and VPS36 are downregulated in advanced prostate cancer and show functional relevance in prostate cancer cells
- Author
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Cleo-Aron Weis, Annette Steidler, Yannic Meyer, Thomas Stefan Worst, Maurice Stephan Michel, Christine Frank, Philipp Erben, Maria Gottschalt, and Jost von Hardenberg
- Subjects
Male ,0301 basic medicine ,Cancer Research ,Microarray ,Down-Regulation ,Biology ,medicine.disease_cause ,rab27 GTP-Binding Proteins ,03 medical and health sciences ,Paracrine signalling ,Prostate cancer ,0302 clinical medicine ,Cancer stem cell ,Cell Line, Tumor ,Biomarkers, Tumor ,medicine ,Humans ,RNA, Small Interfering ,Cell Proliferation ,Gene knockdown ,Endosomal Sorting Complexes Required for Transport ,Oncogene ,Prostate ,Prostatic Neoplasms ,Cancer ,Middle Aged ,medicine.disease ,030104 developmental biology ,Oncology ,rab GTP-Binding Proteins ,030220 oncology & carcinogenesis ,Cancer research ,RNA Interference ,Carcinogenesis - Abstract
Paracrine and long-range signaling via extracellular vesicles, such as exosomes and microvesicles, is deemed crucial for tumorigenesis, invasion and spread of solid tumors. The ESCRT machinery (endosomal sorting complexes required for transport) and Rab-proteins act as key players in vesicular trafficking and secretion. Yet, their role in prostate cancer (PCa) is unknown. Therefore, this study aimed to elucidate the relevance of these components in PCa. In silico reanalysis of genes with known involvement in vesicular trafficking and secretion in an existing microarray dataset revealed low expression of RAB27A, RAB27B and VPS36 to be predictive for reduced BCR-free survival in patients with localized PCa (p=0.033, 0.025 and 0.005). In the same microarray dataset underexpression of RAB27A, RAB27B and VPS36 was seen in distant metastases (p
- Published
- 2017
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