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Hydrogen sulfide (H2S), as one of the important endogenous biological regulators, plays a critical role in mediating a wide range of physiological processes. The development of rapid, sensitive, and reliable detection techniques for H2S would be highly appealing. In this paper, a new type of AIE‐based fluorescence turn‐on probe TPA‐M for the detection of H2S has been constructed, involved the rapid release of AIE‐based fluorophore TPA‐CHO with a remarkable fluorescence turn‐on phenomenon in THF/H2O (2/8, v/v, HEPES=20 μM, pH=7.3) medium, exhibiting the attractive advantages such as high sensitivity with the detection limit as low as 1.92×10−3 ppm, excellent selectivity over other anion analytes and biothiols, significant anti‐interference ability and fast response time (within 10 min). What's more, the practical application evaluation indicated that the probe TPA‐M could be efficiently employed in imaging exogenously added H2S in living MCF‐7 cells and detecting H2S in actual water and wine samples. [ABSTRACT FROM AUTHOR]

Objective: To obtain the impacts of experimental design on benchmark dose (BMD), and the result was applied to test the computer simulation by software Slob (optimal method to calculate the BMD: for a certain sample capacity, to add the experimental groups by reducing the amount of animals in each group) , consequently, this method can be widely used in the future., Methods: Eighty adult female SD rats were ig given dimethoate 0.5, 1, 2, 4, 8, 16 and 32 mg/kg for 21 d, respectively. Rats were sacrificed, and acetylcholinesterase (AChE) activity in the hippocampus, cerebral cortex and serum of rats was determined after dimethoate was ig given to rats for 21 d. And then, the software package PROAST28.1 was applied to calculate the BMD. The four does groups of 10 animals (4 x 10 design) and 8 x 5 design were selected from 8 x 10 design to study the impacts of experimental design on BMD., Results: Comparing with the normal control, the significant decline of AChE in hippocampus was observed in 2, 4, 8, 16 and 32 mg/kg groups (P < 0.05), whereas the significant decrease was obtained in 0.5, 1, 2, 4, 8, 16 and 32 mg/kg groups (P < 0.05). Taking the 8 x 10 design as the standard, the confidence interval of BMD calculated by both of 4 x 10 design and 8 x 5 design covered the BMD by 8 x 10 design. And also, confidence interval of BMD, calculated by design scheme 1, 2, 3, 4 and 6 of 4 x 10 design, wider than that of 8 x 5 design, but its scheme 5 narrower than 8 x 5 design., Conclusion: To add experimental groups in a certain sample capacity was the optimal method to calculate BMD, but was not the common toxicity experimental design (e. g. set four groups including control, low-dose, moderate-dose, high-dose group).

Objective: To understand the cumulative dietary exposure of Jiangsu residents to organophosphorus (OPs) pesticide and make acute risk assessment., Methods: Integrated the data of the nutrition and health status of residents in Jiangsu and the data of monitoring of OPs pesticide in agricultural products. Chlorpyrifos was selected as index compound (index chemical, IC), then use relative potency factor (RPF) approach which commended by EPA and simple distribution evaluation. Caloulated the dietary cumulative exposure of OPs pesticide among Jiangsu residents and compared with acute reference dose (ARfD), then made risk assessment., Results: The exposure of rural group of age 3-6 and 7-11 were 133.84 microg/kg BW and 154.32 microg/kg BW, exceeded ARfD. The exposure level of kids and elder was higher than adults. The exposure level of rural residents were higher than urban residents. The highest contribution to the food of each age group was greengrocery and leek., Conclusion: The average level of exposure was safety in Jiangsu, high exposure children were at acute poisoning risk. High contribution food such as greengrocery and leek should be strengthen monitoring.