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7 results on '"Yashi Zhang"'

1. Preparation and Antioxidant Activity Evaluation of Jerusalem Artichoke Polysaccharide-Zn Complex

Author

Yashi ZHANG, Wenwen LI, Aizhen ZONG, Zhaohe ZUO, Zhenjia ZHENG, and Bin ZHANG

Subjects
jerusalem artichoke, polysaccharide, zinc, process optimization, antioxidant activity, Food processing and manufacture, TP368-456
Abstract

Jerusalem artichoke polysaccharide (JAP)-Zn complex was prepared using JAP and ZnSO4 as raw materials, and single factor and response surface methodology methods were applied for the optimization preparation process of JAP-Zn complex. Besides, the antioxidant activities in vitro of JAP and JAP-Zn complex were investigated. The optimal preparation process of the JAP-Zn complex was as follows: The weight ratio of JAP and ZnSO4 was 32:1, the reaction time was 60 min, the reaction temperature was 50 °C, and the reaction pH was 8.50. Under these conditions, the chelation rate was 87.06%±0.28%. The results of antioxidant activity in vitro showed that both JAP and JAP-Zn complex exhibited excellent antioxidant activity in the concentration range of 0.3~2.7 mg/mL. The maximum scavenging capacity of JAP-Zn complex on the maximum scavenging rates of DPPH free radical, hydroxyl free radical and superoxide anion free radical, ABTS+ free radical was 23.42%, 13.47%, 29.36% and 18.50%, respectively. The results would provide a theoretical basis for the development of functional foods and nutritional supplements for JAP and JAP-Zn complex.

Published
2023
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4. Phosphorylation of an ethylene response factor by MPK3/MPK6 mediates negative feedback regulation of pathogen-induced ethylene biosynthesis in Arabidopsis

Author

Xiaoyang Wang, Huicong Meng, Yuxi Tang, Yashi Zhang, Yunxia He, Jinggeng Zhou, and Xiangzong Meng

Subjects
Defensins, Mitogen-Activated Protein Kinase Kinases, Arabidopsis Proteins, Gene Expression Regulation, Plant, Arabidopsis, Genetics, Ethylenes, Mitogen-Activated Protein Kinases, Phosphorylation, Molecular Biology, Feedback
Abstract

Plants under pathogen attack produce high levels of the gaseous phytohormone ethylene to induce plant defense responses via the ethylene signaling pathway. The 1-aminocyclopropane-1-carboxylate synthase (ACS) is a critical rate-limiting enzyme of ethylene biosynthesis. Transcriptional and post-translational upregulation of ACS2 and ACS6 by the mitogen-activated protein kinases MPK3 and MPK6 are previously shown to be crucial for pathogen-induced ethylene biosynthesis in Arabidopsis. Here, we report that the fungal pathogen Botrytis cinerea-induced ethylene biosynthesis in Arabidopsis is under the negative feedback regulation by ethylene signaling pathway. The ethylene response factor ERF1A is further found to act downstream of ethylene signaling to negatively regulate the B. cinerea-induced ethylene biosynthesis via indirectly suppressing the expression of ACS2 and ACS6. Interestingly, ERF1A is shown to also upregulate defensin genes directly and therefore promote Arabidopsis resistance to B. cinerea. Furthermore, ERF1A is identified to be a substrate of MPK3 and MPK6, which phosphoactivate ERF1A to enhance its functions in suppressing ethylene biosynthesis and inducing defensin gene expression. Taken together, our data reveal that ERF1A and its phosphorylation by MPK3/MPK6 not only mediate the negative-feedback regulation of the B. cinerea-induced ethylene biosynthesis, but also upregulate defensin gene expression to increase Arabidopsis resistance to B. cinerea.

Published
2022

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