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8. Isoproterenol directs hair follicle-associated pluripotent (HAP) stem cells to differentiate in vitro to cardiac muscle cells which can be induced to form beating heart-muscle tissue sheets

Author

Yamazaki, Aiko, primary, Yashiro, Masateru, additional, Mii, Sumiyuki, additional, Aki, Ryoichi, additional, Hamada, Yuko, additional, Arakawa, Nobuko, additional, Kawahara, Katsumasa, additional, Hoffman, Robert M., additional, and Amoh, Yasuyuki, additional

Published
2016
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11. Isoproterenol directs hair follicle-associated pluripotent (HAP) stem cells to differentiate in vitroto cardiac muscle cells which can be induced to form beating heart-muscle tissue sheets

Author

Yamazaki, Aiko, Yashiro, Masateru, Mii, Sumiyuki, Aki, Ryoichi, Hamada, Yuko, Arakawa, Nobuko, Kawahara, Katsumasa, Hoffman, Robert M., and Amoh, Yasuyuki

Abstract

ABSTRACTNestin-expressing hair-follicle-associated pluripotent (HAP) stem cells are located in the bulge area of the follicle. Previous studies have shown that HAP stem cells can differentiate to neurons, glia, keratinocytes, smooth muscle cells, and melanocytes in vitro. HAP stem cells effected nerve and spinal cord regeneration in mouse models. Recently, we demonstrated that HAP stem cells differentiated to beating cardiac muscle cells. The differentiation potential to cardiac muscle cells was greatest in the upper part of the follicle. The beat rate of the cardiac muscle cells was stimulated by isoproterenol. In the present study, we observed that isoproterenol directs HAP stem cells to differentiate to cardiac muscle cells in large numbers in culture compared to HAP stem cells not supplemented with isoproterenol. The addition of activin A, bone morphogenetic protein 4, and basic fibroblast growth factor, along with isoproternal, induced the cardiac muscle cells to form tissue sheets of beating heart muscle cells. These results demonstrate that HAP stem cells have great potential to form beating cardiac muscle cells in tissue sheets.

Published
2016
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12. Protocols for Efficient Differentiation of Hair Follicle-Associated Pluripotent (HAP) Stem Cells to Beating Cardiac Muscle Cells.

Author

Yashiro M, Mii S, Aki R, Hamada Y, Arakawa N, Kawahara K, Hoffman RM, and Amoh Y

Subjects
Animals, Biomarkers, Fluorescent Antibody Technique, Immunophenotyping, Mice, Pluripotent Stem Cells metabolism, Vibrissae, Cell Differentiation, Hair Follicle cytology, Myocytes, Cardiac cytology, Myocytes, Cardiac physiology, Pluripotent Stem Cells cytology
Abstract

We have previously demonstrated that the nestin-expressing cells from the upper part of the hair follicle can differentiate to neurons and other cell types. We have termed these cells as hair-associated-pluripotent (HAP) stem cells. In the present chapter, we describe methods for HAP stem cells to differentiate to beating cardiac muscle cells. The mouse vibrissa hair follicle was divided into three parts (upper, middle, and lower), and each part was suspended separately in DMEM containing 10 % fetal bovine serum (FBS). All three parts of hair follicle differentiate to neurons, glial cells, keratinocytes, smooth muscle cells, and cardiac muscle cells. The differentiation potential to cardiac muscle is greatest in the upper part of the follicle. Hair spheres comprised of HAP stem cells formed from the upper part of vibrissa hair follicle can differentiate to cardiac muscle cells.

Published
2016
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