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Abstract Multidrug resistance (MDR) is a major obstacle limiting the effectiveness of chemotherapy against cancer. The combination strategy of chemotherapeutic agents and siRNA targeting drug efflux has emerged as an effective cancer treatment to overcome MDR. Herein, stimuli‐responsive programmable tetrahedral DNA‐RNA nanocages (TDRN) have been rationally designed and developed for dynamic co‐delivery of the chemotherapeutic drug doxorubicin and P‐glycoprotein (P‐gp) siRNA. Specifically, the sense and antisense strand sequences of the P‐gp siRNA, which are programmable bricks with terminal disulfide bond conjugation, are precisely embedded in one edge of the DNA tetrahedron. TDRN provides a stimuli‐responsive release element for dynamic control of functional cargo P‐gp siRNA that is significantly more stable than the “tail‐like” TDN nanostructures. The stable and highly rigid 3D nanostructure of the siRNA‐organized TDRN nanocages demonstrated a notable improvement in the stability of RNase A and mouse serum, as well as long‐term storage stability for up to 4 weeks, as evidenced by this study. These biocompatible and multifunctional TDRN nanocarriers with gold nanocluster‐assisted delivery (TDRN@Dox@AuNCp) are successfully used to achieve synergistic RNAi/Chemo‐therapy in vitro and in vivo. This programmable TDRN drug delivery system, which integrates RNAi therapy and chemotherapy, offers a promising approach for treating multidrug‐resistant tumors.

ABSTRACTDouble minutes (DMs), extrachromosomal gene fragments found within certain tumors, have been noted to carry onco- and drug resistance genes contributing to tumor pathogenesis and progression. After screening for SUMO-related molecule expression within various tumor sample and cell line databases, we found that SUMO-conjugating enzyme UBC9 has been associated with genome instability and tumor cell DM counts, which was confirmed both in vitro and in vivo. Karyotyping determined DM counts post-UBC9 knockdown or SUMOylation inhibitor 2-D08, while RT-qPCR and Western blot were used to measure DM-carried gene expression in vitro. In vivo, fluorescence in situ hybridization (FISH) identified micronucleus (MN) expulsion. Western blot and immunofluorescence staining were then used to determine DNA damage extent, and a reporter plasmid system was constructed to detect changes in homologous recombination (HR) and non-homologous end joining (NHEJ) pathways. Our research has shown that UBC9 inhibition is able to attenuate DM formation and lower DM-carried gene expression, in turn reducing tumor growth and malignant phenotype, via MN efflux of DMs and lowering NHEJ activity to increase DNA damage. These findings thus reveal a relationship between heightened UBC9 activity, increased DM counts, and tumor progression, providing a potential approach for targeted therapies, via UBC9 inhibition.

Abstract The growth of newly formed particles through new particle formation (NPF) contributes a significant fraction to the cloud condensation nuclei, yet the driving mechanisms remain unclear, especially for polluted environments. To investigate the potential species contributing for nanoparticle growth in environments with significant anthropogenic influences, we measured the hygroscopicity of newly formed particles at 20–40 nm at a rural observational site in the North China Plain during winter 2018. Our results demonstrate that these particles were not very hygroscopic, with the mean hygroscopicity parameter κ of 0.13 ± 0.09. Clear differences in the inferred κ of the growing material responsible for the growth were observed among different events, indicating that even at the same region, the compounds driving particle growth may not be identical. This may be synergistically influenced by the NPF precursors, oxidants and meteorological conditions, suggesting complex mechanisms might co‐exist behind nanoparticle growth in polluted environments.

Abstract 5-methylcytosine (m5C) is a post-transcriptional RNA modification identified, m5C readers can specifically identify and bind to m5C. ALYREF and YBX1 as members of m5C readers that have garnered increasing attention in cancer research. However, comprehensive analysis of their molecular functions across pancancer are lacking. Using the TCGA and GTEx databases, we investigated the expression levels and prognostic values of ALYREF and YBX1. Additionally, we assessed the tumor microenvironment, immune checkpoint-related genes, immunomodulators, Tumor Immune Dysfunction and Exclusion (TIDE) score and drug resistance of ALYREF and YBX1. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) analyses were performed to investigate the potential functions associated with m5C readers and coexpressed genes. Aberrant expression of ALYREF and YBX1 was observed and positively associated with prognosis in KIRP, LGG and LIHC. Furthermore, the expression levels of ALYREF and YBX1 were significantly correlated with immune infiltration of the tumor microenvironment and immune-related modulators. Last, our analysis revealed significant correlations between ALYREF, YBX1 and eIFs. Our study provides a substantial understanding of m5C readers and the intricate relationship between ALYREF, YBX1, eIFs, and mRNA dynamics. Through multidimensional analysis of immune infiltration and drug sensitivity/resistance in ALYREF and YBX1, we propose a possibility for combined modality therapy utilizing m5C readers.

Abstract This study introduces an innovative outdoor nephelometer system designed to monitor the dynamic hygroscopic behavior of aerosols in ambient air. Field measurements conducted in the Pearl River Delta region of China unveil significant roles of relative humidity (RH) swings on aerosol phase states. Highlighting the occurrence of aerosol crystallization in the afternoon followed by gradual deliquescence as RH increases, particularly when minimum afternoon RH drops below 35%, with no such behavior observed when it exceeds 40%. Emphasizing that aerosol phase states are shaped not only by RH and chemical composition but also by their RH history, illustrating that RH levels of 70% in the morning or evening may correspond to fully dissolved metastable or partially dissolved metastable states of ambient aerosols. These findings underscore the need to account for RH history when predicting aerosol phase states and have broader implications for comprehending aerosol behavior and its atmospheric impacts.

Abstract Activation of biomass burning aerosols (BBA) and fossil fuel combustion aerosols (FFA) in fogs and clouds significantly impact regional air quality through aqueous chemistry and climate by affecting cloud microphysics. However, we lack direct observations of how these aerosols behave in fogs and clouds. Using a newly developed aerosol‐cloud sampling system, we conducted observations during fog events and found that BBA, despite their high organic content, effectively contributed to super‐micron interstitial aerosols and fog droplets in low supersaturation fogs. In contrast, FFA, predominantly externally mixed organic, did not grow beyond the super‐micron size in fogs due to their near‐hydrophobic nature. Measurements conducted under supersaturations relevant for cloud formation revealed that portions of FFA could serve as cloud condensation nuclei, but only when supersaturation exceeded ∼0.14%. These findings have broad implications for future investigations into the influence of BBA and FFA on fog and cloud chemistry and their interactions with clouds.

Abstract Background Hepatocellular carcinoma (HCC) is a primary liver cancer with high mortality. The long‐term use of sorafenib, a targeted drug for hepatocellular carcinoma, will lead to drug resistance, and patients are prone to cancer metastasis, the molecular mechanism of which is still unclear. Methods In our study, we constructed a sorafenib‐resistant hepatocellular carcinoma cell line (HepG2/Sora) and validated the resistance in vivo and in vitro. Transwell assays confirmed the invasion and migration abilities of cells. Colorimetric assays confirmed that the level of m6A methylation modification in cells. RT‐qPCR and Western blot assays confirmed that the expression levels of KIAA1429 in HepG2/Sora cells and tissues. The EMT related proteins were detected by Western blot assay. Results Transwell and Western blot assays confirmed that HepG2/Sora cells had higher invasion and migration abilities and showed EMT phenomena. Colorimetric assays confirmed that the level of m6A methylation modification was upregulated in HepG2/Sora cells. Transwell and Western blot assays confirmed that inhibiting m6A methylation in HepG2/Sora cells inhibited their invasion, migration ability and EMT phenomenon. RT‐qPCR and Western blot assays confirmed that the expression levels of KIAA1429 in HepG2/Sora cells and tissues was increased. Silencing KIAA1429 in HepG2/Sora cells inhibited their invasion, migration ability and EMT phenomenon. Finally, we found that the medium supernatant of sorafenib‐resistant HepG2/Sora cells induced vascular production of EA.hy926 cells, and silencing KIAA1429 inhibited this induction effect. Conclusion We suggest that KIAA1429 promotes sorafenib‐resistant hepatocellular carcinoma invasion, migration and EMT by mediating m6A methylation. KIAA1429 with its mediated m6A methylation may be a key factor for sorafenib‐resistant patients prone to cancer cell metastasis.

Solar radiation triggers atmospheric nitrous acid (HONO) photolysis, producing OH radicals, thereby accelerating photochemical reactions, leading to severe secondary pollution formation. Missing daytime sources were detected in the extensive HONO budget studies carried out in the past. In the rural North China Plain, some studies attributed those to soil emissions and more recent studies to dew evaporation. To investigate the contributions of these two processes to HONO temporal variations and unknown production rates in rural areas, HONO and related field observations obtained at the Gucheng Agricultural and Ecological Meteorological Station during spring and autumn were thoroughly analyzed. Morning peaks in HONO frequently occurred simultaneously with those of ammonia (NH3) and water vapor both during spring and autumn, which were mostly caused by dew and guttation water evaporation. In spring, the unknown HONO production rate revealed pronounced afternoon peaks exceeding those in the morning. In autumn, however, the afternoon peak was barely detectable compared to the morning peak. The unknown afternoon HONO production rates were attributed to soil emissions due to their good relationship to soil temperatures, while NH3 soil emissions were not as distinctive as dew emissions. Overall, the relative daytime contribution of dew emissions was higher during autumn, while soil emissions dominated during spring. Nevertheless, dew emission remained the most dominant contributor to morning time HONO emissions in both seasons, thus being responsible for the initiation of daytime OH radical formation and activation of photochemical reactions, while soil emissions further maintained HONO and associated OH radial formation rates at a high level, especially during spring. Future studies need to thoroughly investigate the influencing factors of dew and soil emissions and establish their relationship to HONO emission rates, form reasonable parameterizations for regional and global models, and improve current underestimations in modeled atmospheric oxidation capacity.

On-orbit assembling space telescope (OAST) is one of the most feasible methods to implement a large-scale space telescope. Unlike a monolithic space telescope (such as Hubble Space Telescope, HST) or a deployable space telescope (such as James Webb Space Telescope, JWST), OAST can be assembled in the spatial environment. To ensure proper telescope performance, OAST must be equipped with a large deployable sunshade. In order to verify the technology of the OAST, the authors propose a modular space telescope on the China Space Station (CSS) and design a deployable sunshade. The deployable mechanism of the sunshade is made up of a radial deployable mechanism and an axial deployable mechanism. The paper describes the overall design approach, the key component technologies, and the design and preliminary testing of a part of the deployable sunshade assembly.

Abstract The commercial continuous‐flow streamwise thermal‐gradient cloud condensation nuclei (CCN) counter is widely used in aerosol CCN activity measurements which are critical for investigating aerosol‐cloud interactions. In CCN measurements, a critical threshold is needed for distinguishing interstitial aerosols and activated droplets, and a default threshold of 0.75 μm was set in CCN counter. Theoretically, interstitial aerosols could also grow larger than 0.75 μm in CCN counter at low supersaturations, thus theoretical thresholds derived from the Köhler theory were suggested. Here, we report that droplet growth in the CCN counter is kinetically limited and CCN‐active droplets does not reach their theoretical diameters under low supersaturations. Neglecting the kinetic growth limitation in CCN identification would count less CCN and underestimate the aerosol hygroscopicity parameter κ by up to 50% for supersaturations lower than 0.1%. We recommend that thresholds considering kinetic limitations should be used as new criteria for CCN identification under low supersaturations.

Inland surface water plays an important role in global carbon cycling, which responds to transformation between dissolved inorganic carbon (DIC) and dissolved organic carbon (DOC). Studies have shown that algae in karst lakes and reservoirs can convert DIC to organic matter (OM) and form stable carbon sinks via photosynthesis. However, the pathways of conversion of inorganic carbon to organic carbon during algal growth remain unclear and need further investigation. In this study, spectroscopic techniques were applied to investigate the variations in algal organic matter (AOM) composition in the growth metabolism of Chlorella vulgaris and Scenedesmus obliquus under simulated karst water condition. The results showed that algal extracellular organic matter (EOM) contained high DIC concentration during the adaptation phase, which formed the carbon source for algal photosynthesis. In addition, DOC in algae increased after entering the stationary phase, while more OM was released into water. As algal growth proceeded, the amino groups in EOM were consumed to produce more aromatic protein-like material, while more lipid material was produced in intracellular organic matter (IOM). The spectral characterization results could intuitively determine AOM dynamics in different growth stages of algae, which can be used for establishing effective approaches for detecting organic carbon variations and responding to regional carbon cycling in karst water.

The study of the karst water flow system is helpful for the reasonable evaluation and utilization of groundwater resources.In this paper, the spatial characteristics, hydrological dynamic response, temperature field, and conductivity characteristics of the typical karst water flow system were calculated and quantified by referring to the methods of hydrology research.Four groundwater systems, i.e., fan-shaped, branch-shaped, flat-shaped, and comb-shaped, were divided to comprehensively reflect the surface-underground karst characteristics of the karst flow system.The main pipelines of the first two are perpendicular to the stratigraphic trend, and the structural fractures play a role in catching water.The main pipelines of the latter two are parallel to the stratigraphic trend, and the horizontal fractures play a role in collecting water.The northwest, west, and south wings of the Huangling anticline are branch-shaped and flat-shaped, while the east and north wings are fan-shaped and flat-shaped water systems.The formation and regional differences of different groundwater systems are closely related to the spatial relationship and hierarchy of aquifer systems and water systems which show different dynamic characteristics.The fan-shaped and flat-shaped karst flow system are the most sensitive to rainfall response, while the comb-shaped karst flow system has the slowest response and attenuation process.Based on the obvious correlation between karst groundwater temperature and exposed elevation and circulation depth, the groundwater temperature line in the mountainous area of western Hubei province is established.This study could provide certain theoretical support for karst groundwater flow system research and local engineering practice.

Because direct roasting of aluminum fluoride hydrate will hydrolysis, resulting in higher alumina content in aluminum fluoride products. To solve this problem, a two-step process for the preparation of anhydrous AlF3 from AlF3·3H2O and NH4HF is described in this paper. First, AlF3·3H2O was dried at 220 ℃ for 9–12 h, and then NH4HF (the mass ratio of AlF3·3H2O to NH4HF was 1:20) was added, and the mixture was roasted from room temperature to 650 ℃. The results showed that the hydrolysis of aluminum fluoride can be avoided effectively by the two-step roasting method with NH4HF, the purity of the anhydrous AlF3 was 99.8 wt%, and the concentration of alumina, the main impurity, was less than 0.1 wt%.

The investigation and prevention of karst water inrush is a difficult problem in tunnel construction. The Xiakou Tunnel has a long history of karst water inrush.To identify the source of intermittent water inrush and explore the controlling effect of karst water system to the process of water inrush, the hydrogeological survey, hydrological and hydrogeochemical methods were used to identify the sources and processes of water inrush. The results show that, there is a groundwater divide at the area of Mengjialing, and the groundwater in the northern and southern area discharge into Xianglongdong and Xiakoudong, respectively. In the events of concentrated water inrush, the total amount of event water inrush has a significant linear positive correlation with the rainfall event, and the hydrogeochemical composition of the water inrush is very similar to that of the four blind drainage ditches, which indicates that all these water inrushes and blind drainage ditches come from the karst water in the northern part of the tunnel. During the stable drainage period, the karst water in the northern part of the tunnel is mainly discharged through the blind drainage ditch on the north of the right tunnel, while the groundwater of other three blind drainage ditches come from the fissure water in clastic rock.The concentrated water inrush point is located on the surface of saturated zone in the karst water system of Xiakou. The heavy rainfall events made the upper part of tunnel to be filled with karst water.The water inrush was caused by intercepting the fast flow in karst channel, and small part of base flow was mixed into the water inrush. In the study of karst water inrush, the comprehensive utilization of multi-technical methods and multi-information verifications can improve the accuracy in the source identification of water inrush.

Many factors affect pores in cemented paste backfill (CPB), and there are numerous species of admixtures which have complicated influences on CPB, such as the action of air entraining agents (AEAs). Previously, sodium abietate (SA) and triterpenoid saponins (SJ) have been used as AEAs. In this study, CPB was prepared by mixing different amounts (0%, 0.2%, 0.4% and 0.6%) of AEA with different compounds (tailings, cement etc.). The quantitative relationship of microscopic characterization between the action of AEAs on CPB pore structure was established using nuclear magnetic resonance (NMR), scanning electron microscope (SEM) and fractal theory. Our results indicate that: (1) T2 spectrums of CPB samples tested using NMR all have three peaks, where the proportion of main peaks characterizing micro-pores (the main pore type) was >88%. (2) The addition of AEAs increases CPB porosity, optimizes the pore structure in the CPB sample, and achieves uniform distribution. The proportion of main peaks characterizing micro-pores increased to >90%. (3) In the same period the degree of cement hydration reaction was found to differ when CPBs with or without AEAs were compared. The addition of AEAs has a certain delay hindering effect on the cement hydration reaction. (4) The fractal properties of CPB pores with the addition of AEA are very strong. The fractal dimension of pores is linearly directly proportional to AEA content and porosity, and the complexity of pores increases with the increase of fractal dimension.

The conveying distance during the backfill operation has become extensively large with the advancement of mining work. It requires the immediate attention of academic and industrial communities. Adding air entraining agent (AEA) can improve the rheological properties of the filling slurry and facilitat the filling slurry transfer to the long-distance underground stope. The AEA can generate a large number of tiny bubbles, which acted as floating balls and reduced the friction between the particles. In addition, AEA acted as a dispersing and wetting agent. Herein, we have obtained filling material from the Sanning mine (Hubei, China) and utilized sodium abietate (SA) and triterpenoid saponins (SJ), as air entraining agents, to improve the fluidity of filling slurry. The influence of AEA content on mechanical properties, structure and morphology has been analyzed by uniaxial compressive strength (UCS) testing, nuclear magnetic resonance (NMR) and scanning electron microscopy (SEM). The results reveal that the addition of AEA increased the fluidity of filling slurry. Moreover, UCS of the AEA-free cemented paste backfill (CPB) sample, cured for 28 days, was found to be 2.23 MPa. However, the addition of 0.2% AEA (SA and SJ) increased the UCS to 2.31 MPa and 2.35 MPa, respectively. However, when SA and SJ amount was increased to 0.6%, UCS decreased to 2.19 MPa and 2.13 MPa, respectively. Hence, UCS of the CPB initially increased with the addition of AEA, followed by a gradual decrease, which can be ascribed to the pore structure inside CPB. One should note that the presence of AEA increased the number of small pores inside the CPB. The SEM results shows that pore structure of CPB can be optimized by adding an optimal proper amount of AEA.

The blastability grading of a rock mass is an important parameter for the blasting design in metal mines. Appropriate blastability grading results guide the production to increase the efficiency and reduce the costs. In this paper, the multi-factors index system of the rock mass blastability consisting of the density, the p-wave velocity, the wave impedance, the uniaxial compressive strength, the rock elasticity, and the uniaxial tensile strength is determined for the Tongkeng ore in Guangxi, China. The determination of such indicator system is based on the multi-factors interaction, ambiguity, and randomness of the rock engineering system (RES). Based on the system engineering theory, a new improved RES-multidimensional cloud rock mass blastability classification model is established. The results show that the cloud-inference expert semi-quantitative (CESQ) can effectively obtain the expert experience and decrease the subjectivity of the traditional RES methods, and based on the improved fuzzy RES, the interaction factors among the factors and the systems affecting the blasting morphology of the rock mass are analyzed. Moreover, the importance of in situ testing of mechanical parameters of different rock masses is determined; finally, the five 6-D cloud models for the rock mass blastability grade are generated via the multidimensional cloud theory. The program is designed to support the engineering case analysis and is consistent with the results of the sample blasting experiment. A biased evaluation of the edge results is carried out, and this improved the actual usability of the evaluation model.

To control pores in the backfill, the air-entraining agents (AEAs) are used as an admixture to realize the pore structure changes under artificial action and explore the effect of pore structure on strength. Two AEAs at different dosages were added to the backfill. The relationship was then analyzed between them from the macro- and mesoscopic aspects. The results indicate that AEA can regulate pore structure changes of AEACPB. With the increase in AEA content, the total pore volume of different pore sizes in AEACPB increases, in which the proportion of big and medium pore gradually increases while the proportion of small pore gradually decreases. The AEACPB’s UCS is linearly negatively correlated with the porosity and pore percentage, which is the primary factor affecting the AEACPB of the pore structure. When the total pores’ volume in the AEACPB is constant, the influence of different pore structures differs. A higher proportion of small pores leads to a linear increase in strength; a higher proportion of medium pores leads to a linear decrease in strength; and a higher proportion of big pores leads to an exponential decrease in strength. And the fractal dimension has a linear negative correlation with the UCS by fractal theory analysis.

Under different exploitive conditions, the humidity levels of the backfill stopes are not the same. Humidity greatly affects the strength and microscopic characterization of the backfill. Cemented paste backfill (CPB) specimens were cured using 0, 30, 70% and standard curing (20°C, 99%) under four different humidity conditions. At 28 days, nuclear magnetic resonance (NMR) and scanning electron microscopy (SEM) techniques were used to obtain the microscopic features of the CPB specimens. The relationships between the permeability and uniaxial compressive strength (UCS) of the CPB specimens, and the microscopic characteristics of the CPB specimens, were established. The results showed the following: (i) The permeability of the CPB had an exponential functional relationship with its stone powder content. (ii) The stone powder content of CPB and the peak area of the T2 spectrum are negatively correlated with the UCS. However, there was a T2 peak area corresponding to the worst UCS with the same stone powder content. (iii) The peak area of the T2 spectrum showed that the proportion of area of a small pore size was more than 80%, indicating that the pore size was mainly small. The pore diameter of small pores was linearly and inversely proportional to the UCS of the specimens. It can be found that the factors affecting the strength characteristics of CPB include not only the stone powder content, but also the curing conditions of different humidity.

Abstract Most cancer cells perform glycolysis despite having sufficient oxygen. The specific metabolic pathways of cancer cells have become the focus of cancer treatment. Recently, accumulating evidence indicates oxidative phosphorylation (OXPHOS) and glycolysis can be regulated with each other. Thus, we suggest that the glycolysis of cancer cells is inhibited by restoring or improving OXPHOS in cancer cells. In our study, we found that oxaloacetate (OA) induced apoptosis in HepG2 cells in vivo and in vitro. Meanwhile, we found that OA induced a decrease in the energy metabolism of HepG2 cells. Further results showed that the expression and activity of glycolytic enzymes were decreased with OA treatment. Conversely, the expression and activity of enzymes involved in the TCA cycle and OXPHOS were increased with OA treatment. The results indicate that OA can inhibit glycolysis through enhancement of OXPHOS. In addition, OA‐mediated suppression of HIF1α, p‐Akt, and c‐myc led to a decrease in glycolysis level. Therefore, OA has the potential to be a novel anticancer drug.

Background/Aims: Non-small cell lung cancer (NSCLC) is the leading cause of death worldwide. Although aquaporin-3 (AQP3) is widely distributed in mammalian tissues and over-expressed in NSCLC cells, there are limited studies on the effects of AQP3 knockdown on NSCLC cells under hypoxic conditions. Methods: The CCK-8 assay was used to calculate cell viability. Scratch-wound healing and transwell assays were used to detect cell migration and invasion. Apoptotic cells were measured by the TUNEL assay. mRNA expression levels were calculated via quantitative RT-PCR. Relative protein levels were determined by immunoblot assays. Results: AQP3 knockdown substantially reduced proliferation, migration, and invasion of A549 and NCI-H460 cells under hypoxic conditions. Moreover, AQP3 knockdown clearly induced cell apoptosis. Further analysis identified levels of HIF-1α, VEGF, Raf, phosphor-MEK, and phosphor-ERK, whose activities were significantly attenuated in the AQP3 knockdown group. Conclusions: These findings indicate that AQP3 knockdown retards the growth of NSCLC cells partially through inhibiting HIF-1α/VEGF and Raf/MEK/ERK signalling pathways.

At present, many mines adopt the filling method. It is particularly important to solve the problem of the long-distance transportation of slurry during the filling process. Based on the high-density filling material of Sanning mine, the experiments were designed to add sodium abietate (SA), triterpene saponin (SJ) and sodium dodecyl sulfonate (K12) with concentrations of 0.0%, 0.2%, 0.4% and 0.6%, respectively, which were used as air entraining agents (AEA). The filling body with the curing age of 7 and 28 days was prepared for various tests, including nuclear magnetic resonance (NMR), and alternating current (AC) impedance tests. The effects of the air entraining agent and curing time on the physical properties, pore structure and AC impedance properties of the filling were obtained. The results show that: (1) within the frequency range of 10−1–105 Hz, the variation trend of AC impedance of the filling body cannot be changed by adding the air entraining agent, and the filling body with the same ratio had a similar topological structure. (2) The filling body with different AEA and curing times can be represented by the same equivalent circuit model, while the maximum chi-square coefficient was 0.46%. (3) Under the condition of a high frequency of 105 Hz, the porosity and uniaxial compressive strength of the filling body with 7 day curing age were linearly correlated with the AC impedance. However, the porosity and uniaxial compressive strengths of the filling body with 28 days curing time were affected by the type of AEA at a high frequency of 105 Hz.

Layered double hydroxides (LDHs), also well-known as hydrotalcite-like layered clays, have been widely investigated in the fields of catalysts and catalyst support, anion exchanger, electrical and optical functional materials, flame retardants and nanoadditives. This feature article focuses on the progress in micro-/nanostructured LDHs in terms of morphology, and also on the preparations, applications, and perspectives of the LDHs with different morphologies.

Wearable antennas play an important role in transmitting signals wirelessly in body-worn systems, helping body-worn applications to achieve real-time monitoring and improving the working efficiency as well as the life quality of the users. Over conventional antenna types, ultra wideband (UWB) antennas have advantages of very large operating bandwidth, low power consumption, and high data transmission speed, therefore, they become of great interest for body-worn applications. One of the strategies for making the antenna comfortable to wear is replacing the conventional rigid printed circuit board with textile materials in the manufacturing process. In this study, a novel three-dimensional woven fabric integrated UWB antenna was proposed and fabricated with pure textile materials. The antenna electromagnetic properties were simulated and measured and its properties under bending were investigated. The antenna operated in a wide bandwidth from 2.7 to 13 GHz with the proper radiation pattern and gain value. At the same time, the antenna performance under bending varied in a reasonable range indicating that the antenna is prospectively applied on the curved surfaces of the human body. Additionally, the current distribution of the antenna showed that different conductive parts had different current densities indicating the uniqueness of the three-dimensional textile-based antenna.

Chemical injury is partly due to free radical lipid peroxidation, which can induce oxidative stress and produce a large number of reactive oxygen species (ROS). Oxaloacetic acid is an important intermediary in the tricarboxylic acid cycle (TCA cycle) and participates in metabolism and energy production. In our study, we found that oxaloacetate (OA) effectively alleviated liver injury which was induced by hydrogen peroxide (H2O2) in vitro and carbon tetrachloride (CCl4) in vivo. OA scavenged ROS, prevented oxidative damage and maintained the normal structure of mitochondria. We further confirmed that OA increased adenosine triphosphate (ATP) by promoting the TCA production cycle and oxidative phosphorylation (OXPHOS). Finally, OA inhibited the mitogen-activated protein kinase (MAPK) and apoptotic pathways by suppressing tumor necrosis factor-α (TNF-α). Our findings reveal a mechanism for OA ameliorating chemical liver injury and suggest a possible implementation for preventing the chemical liver injury.

Inertial sensors are widely used in various applications, such as human motion monitoring and pedestrian positioning. However, inertial sensors cannot accurately define the process of human movement, a limitation that causes data drift in the process of human body positioning, thus seriously affecting positioning accuracy and stability. The traditional pedestrian dead-reckoning algorithm, which is based on a single inertial measurement unit, can suppress the data drift, but fails to accurately calculate the number of walking steps and heading value, thus it cannot meet the application requirements. This study proposes an indoor dynamic positioning method with an error self-correcting function based on the symmetrical characteristics of human motion to obtain the definition basis of human motion process quickly and to solve the abovementioned problems. On the basis of this proposed method, an ultra-wide band (UWB) method is introduced. An unscented Kalman filter is applied to fuse inertial sensors and UWB data, inertial positioning is applied to compensation for the defects of susceptibility to UWB signal obstacles, and UWB positioning is used to overcome the error accumulation of inertial positioning. The above method can improve both the positioning accuracy and the response of the positioning results. Finally, this study designs an indoor positioning test system to test the static and dynamic performances of the proposed indoor positioning method. Results show that the positioning system both has high accuracy and good real-time performance.

Pichiapastoris is one of the most widely used expression systems for the production of recombinant secretory proteins. Its universal application is, however, somewhat hampered by its unpredictable yields for different heterologous proteins, which is now believed to be caused in part by their varied efficiencies to traffic through the host secretion machinery. The yeast endoprotease Kex2 removes the signal peptides from pre-proteins and releases the mature form of secreted proteins, thus, plays a pivotal role in the yeast secretory pathways. In this study, we found that the yields of many recombinant proteins were greatly influenced by Kex2 P1' site residues and the optimized P1's amino acid residue could largely determine the final amount of secretory proteins synthesized and secreted. A further improvement of secretory yield was achieved by genomic integration of additional Kex2 copies, which again highlighted the importance of Kex2 cleavage to the production of recombinant secretory proteins in Pichia yeast.

Aerosol hygroscopic growth and activation under high relative humidity (RH) conditions significantly influence the physicochemical properties of submicron aerosols (PM1). However, this process remains poorly characterized due to limited measurements. To address this gap, we deployed an advanced aerosol-fog sampling system that automatically switched between PM1, PM2.5, and TSP inlets at a rural site in the North China Plain in cold season. The results revealed that aerosol swelling due to water vapor uptake influenced aerosol sampling under high RH conditions by shifting the cut-off size of impactors. Under subsaturated high RH (> 90%), over 25% of aerosol mass with dry diameters below 1 µm resided in supermicron ranges, while in supersaturated foggy conditions, more than 70% submicron aerosol migrated to supermicron ranges. Hygroscopic growth and activation particularly affected highly hydrophilic inorganic salts shifting a significant mass submicron sulfate and nitrate particles to supermicron ranges, with 27 - 33% under 95% = RH = 99%, and 65.5% in supersaturated foggy conditions. Moreover, more than 10% of submicron biomass burning organic aerosols grew beyond 2.5 µm during fog events, while fossil fuel-related OA (FFOA) remained dominantly in submicron ranges, suggesting inefficient aqueous conversion of FFOA. The two SOA factors (OOA1 and OOA2) behaved differently under supersaturated conditions, with OOA2 exhibiting a higher activated fraction despite a lower oxygen-to-carbon ratio. A substantial increase in organic nitrate and organosulfur mass concentrations in activated droplets during fog events suggested aqueous conversions and formations of brown carbon with potential radiative impacts. Overall, our study highlights remarkably different aqueous processing of primary and secondary PM1 aerosol components under distinct ambient RH conditions. [ABSTRACT FROM AUTHOR]

Aerosol particles play crucial roles in both climate dynamics and human health. However, there remains a significant gap in our understanding of aerosol composition and evolution, particularly regarding secondary organic aerosols (SOA), and their interaction with clouds in high-altitude background areas in China. Here we conducted real-time measurements of submicron aerosols (PM1) using aerosol mass spectrometers at a forested mountain site (1128 m a.s.l.) in southeastern China in November 2022. Our results revealed that organic aerosol (OA) constituted a substantial portion of PM1 (41.1 %), with the OA being primarily of secondary origin, as evidenced by a high oxygen-to-carbon (O/C) ratio (0.85-0.96). Positive matrix factorization resolved two distinct SOA factors: less oxidized oxygenated OA (LO-OOA) and more oxidized OOA (MO-OOA). Interestingly, MO-OOA was scavenged efficiently during cloud events, while cloud evaporation contributed significantly to LO-OOA. The ratio of OA/ACO increased with a decrease in the O/C ratio, suggesting that OA remaining in cloud droplets generally maintained a moderate oxidation state. Furthermore, our results indicated a higher contribution of organic nitrates to total nitrate during cloudy periods (27 %) compared to evaporative periods (3 %). Notably, a substantial contribution of nitrate in PM1 (20.9 %) was observed, particularly during high PM periods, implying that nitrate formed in polluted areas interacted with clouds and significantly impacted the regional background site. Overall, our study underscores the importance of understanding the dynamics of secondary organic aerosols and the impacts of cloud processing in regional mountainous areas in southeastern China. [ABSTRACT FROM AUTHOR]

The interaction between atmospheric aerosols and moisture is crucial for aerosol properties and their climate effects. In this study, thanks to the rich measurements of aerosol properties during the 2016 and 2018 ORACLES campaigns, we investigate the aerosol liquid water content (ALWC) over the south-eastern Atlantic Ocean during the biomass burning (BB) season, as well as the seldom-reported ALWC associated with organic aerosols (OA) (ALWCOA) (OA). ALWCOA is determined using the OA hygroscopicity parameter KOA, derived from in-situ hygroscopicity measurements, particle number size distribution, and chemical composition. The ALWC can be determined either with the overall hygroscopic parameter K/RH) or from the sum of ALWCOA and the ALWC simulated from ISORROPIA-II, a thermodynamic equilibrium model for inorganic aerosol. The ALWC from both methods is highly correlated at all RHs with an R² of 0.99. The ALWC increases with aerosol loading and ambient relative humidity (RH). Due to the lower RH and higher aerosol loading in the 2016 campaign, the ALWC for both campaigns are generally consistent. ALWCOA accounts for 38±16 % of the total ALWC during both campaigns. Notably, the contribution of ALWCOA is greater than commonly reported in the literature, highlighting the significance of OA in ALWC and therefore the aerosol direct radiative forcing in this climatically significant region. The strong correlation between kOA and ALWCOA/ALWC, as indicated by an R² value of 0.72, underscores the importance of a good estimation of kOA in the ALWC estimation. Additionally, the significant difference between ALWCOAvalues calculated using real-time kOA and those calculated with the campaign mean kOA, highlights the limitation of using a constant kOA value, a practice commonly adopted in climate models. [ABSTRACT FROM AUTHOR]