21 results on '"Ye-Jing Rong"'
Search Results
2. Molecular Characterization of E-Type Prostanoid Receptor 4 (EP4) from Ayu (Plecoglossus altivelis) and Its Functional Analysis in the Monocytes/Macrophages.
- Author
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Ye-Jing Rong, Xin-Jiang Lu, and Jiong Chen
- Subjects
Medicine ,Science - Abstract
Prostaglandin E2 (PGE2) plays an important role in a broad spectrum of physiological and pathological processes by interacting with E-type prostanoid receptors (EPs). EP4 is one of four EP subtypes known to mediate the immune response in mammalian monocytes/macrophages. However, the precise function and characteristics of EP4 in fish remain unclear. In this study, we characterized a novel EP4-like (PaEP4L) gene from ayu, Plecoglossus altivelis. The cDNA sequence of PaEP4L is 2781 nucleotides (nts) in length, encoding a polypeptide of 459 amino acid residues with a calculated molecular weight of 51.17 kDa. Sequence comparison and phylogenetic tree analysis showed that PaEP4L shared 76% amino acid identity with that of the Atlantic salmon (Salmo salar). PaEP4L mRNA was detected by real-time quantitative PCR (QPCR) in all tested tissues and head kidney-derived monocytes/macrophages (MO/MФ). It varied greatly in liver, spleen and MO/MФ upon Vibrio anguillarum infection. Western blot analysis revealed a significant increase of PaEP4L in cell homogenates from ayu MO/MФ upon V. anguillarum infection. Moreover, anti-PaEP4L IgG reversed the down-regulation of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) mRNA expression as well as phagocytosis in ayu MO/MФ caused by PGE2. There were no significant differences in the respiratory burst response between PGE2 treated and untreated cells. We further found that cAMP mediated PGE2/PaEP4L signal in ayu MO/MФ. In conclusion, our results indicate that PaEP4L mediates PGE2 effects on ayu MO/MФ function, revealing that EP4 also plays a role in the modulation of cells of the fish's innate immune system.
- Published
- 2016
- Full Text
- View/download PDF
3. CXCR3.1 and CXCR3.2 Differentially Contribute to Macrophage Polarization in Teleost Fish
- Author
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Qiang Chen, Ye-Jing Rong, Jiong Chen, Xin-Jiang Lu, and Feng Chen
- Subjects
Fish Proteins ,STAT3 Transcription Factor ,0301 basic medicine ,Gene isoform ,Carps ,Receptors, CXCR3 ,Green pufferfish ,Immunology ,Macrophage polarization ,Monocytes ,CXCR3 Gene ,03 medical and health sciences ,Chemokine receptor ,0302 clinical medicine ,Immune system ,Phagocytosis ,stomatognathic system ,Animals ,Protein Isoforms ,Immunology and Allergy ,Cloning, Molecular ,Phylogeny ,Regulation of gene expression ,biology ,Tetraodontiformes ,Macrophages ,Fishes ,Cell Differentiation ,biology.organism_classification ,Cell biology ,STAT1 Transcription Factor ,030104 developmental biology ,Gene Expression Regulation ,Osmeriformes ,Plecoglossus altivelis ,030215 immunology - Abstract
The study of multiple copies of chemokine receptor genes in various teleosts has long appealed to investigators seeking to understand the evolution of the immune system. The CXCR CXCR3 gene has two isoforms, CXCR3.1 and CXCR3.2, which are both expressed in macrophages. The distinct roles of teleost CXCR3s have not been identified previously. In this article, we found that CXCR3.1 and CXCR3.2 differentially contributed to macrophage polarization in the teleosts: ayu (Plecoglossus altivelis), grass carp (Ctenopharyngodon idella), and spotted green pufferfish (Tetraodon nigroviridis). In ayu macrophages, the P. altivelis CXCR3.1 (PaCXCR3.1) gene was constitutively expressed, whereas the P. altivelis CXCR3.2 (PaCXCR3.2) gene was induced postinfection with Escherichia coli. Upon E. coli infection, PaCXCR3.1+ and PaCXCR3.2+ macrophages showed an M1 and an M2 phenotype, respectively. CXCL9–11-like proteins mediated M1 and M2 polarization by interacting with the PaCXCR3.1 and PaCXCR3.2 proteins on macrophages, respectively. The transcription factors P. altivelis STAT1 and P. altivelis STAT3 were activated in PaCXCR3.1+ and PaCXCR3.2+ macrophages, respectively. Furthermore, the prognosis of septic ayu adoptively transferred with PaCXCR3.2+ macrophages was improved. Our data reveal a previously unknown mechanism for macrophage polarization, suggesting that redundant genes may regulate crucial functions in the teleost immune system.
- Published
- 2017
4. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells
- Author
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Qiang Chen, Hui-Ying Wang, Yu-Hong Shi, Guan-Jun Yang, Jiong Chen, Ning-Yi Xu, Shun Zhang, Xin-Jiang Lu, Ye-Jing Rong, Chao-Hui Yu, and Chang-Hong Li
- Subjects
0301 basic medicine ,Male ,Science ,medicine.medical_treatment ,General Physics and Astronomy ,Receptors, Cell Surface ,CHO Cells ,Biology ,Granulocyte ,General Biochemistry, Genetics and Molecular Biology ,Article ,03 medical and health sciences ,Mice ,Cricetulus ,Granulocyte Colony-Stimulating Factor ,medicine ,Animals ,Humans ,Cell Lineage ,Lectins, C-Type ,Mice, Knockout ,Multidisciplinary ,Tumor Necrosis Factor-alpha ,Macrophages ,hemic and immune systems ,General Chemistry ,Hematopoietic Stem Cells ,Recombinant Proteins ,Cell biology ,Granulocyte colony-stimulating factor ,Mice, Inbred C57BL ,Haematopoiesis ,030104 developmental biology ,medicine.anatomical_structure ,Cytokine ,Gene Expression Regulation ,Leukocytes, Mononuclear ,Intercellular Signaling Peptides and Proteins ,Tumor necrosis factor alpha ,Bone marrow ,Stem cell ,Cell Adhesion Molecules ,Homeostasis - Abstract
Haematopoietic stem cells (HSCs) can differentiate into cells of all lineages in the blood. However, the mechanisms by which cytokines in the blood affect HSC homeostasis remain largely unknown. Here we show that leukocyte cell-derived chemotaxin 2 (LECT2), a multifunctional cytokine, induces HSC expansion and mobilization. Recombinant LECT2 administration results in HSC expansion in the bone marrow and mobilization to the blood via CD209a. The effect of LECT2 on HSCs is reduced after specific depletion of macrophages or reduction of osteolineage cells. LECT2 treatment reduces the tumour necrosis factor (TNF) expression in macrophages and osteolineage cells. In TNF knockout mice, the effect of LECT2 on HSCs is reduced. Moreover, LECT2 induces HSC mobilization in irradiated mice, while granulocyte colony-stimulating factor does not. Our results illustrate that LECT2 is an extramedullar cytokine that contributes to HSC homeostasis and may be useful to induce HSC mobilization., How extramedullar cytokines regulate hematopoietic stem cell (HSC) homeostasis is unclear. Here, the authors show that the cytokine leukocyte cell-derived chemotaxin 2 regulates HSC expansion and mobilisation via tumour necrosis factor and interaction with CD209a in macrophages.
- Published
- 2016
5. Mobilisation and dysfunction of haematopoietic stem/progenitor cells after Listonella anguillarum infection in ayu, Plecoglossus altivelis
- Author
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Jiong Chen, Xin-Jiang Lu, Qiang Chen, and Ye-Jing Rong
- Subjects
0301 basic medicine ,Myeloid ,animal diseases ,Spleen ,Peripheral blood mononuclear cell ,Listonella anguillarum ,Article ,03 medical and health sciences ,Fish Diseases ,Immune system ,medicine ,Animals ,Progenitor cell ,Cells, Cultured ,Multidisciplinary ,biology ,biology.organism_classification ,Hematopoietic Stem Cells ,Haematopoiesis ,030104 developmental biology ,medicine.anatomical_structure ,Osmeriformes ,Immunology ,Leukocytes, Mononuclear ,Plecoglossus altivelis ,Gram-Negative Bacterial Infections ,Listonella - Abstract
Haematopoietic stem/progenitor cells (HSPCs) can mobilise into blood and produce immune cell lineages following stress. However, the homeostasis and function of HSPCs after infection in teleosts are less well known. Here, we report that Listonella anguillarum infection enhances HSPC mobilisation and reduces their differentiation into myeloid cells in ayu (Plecoglossus altivelis), an aquacultured teleost in East Asia. We established a colony-forming unit culture (CFU-C) assay to measure HSPCs using conditioned medium from peripheral blood mononuclear cells stimulated with phytohaemagglutinin. The number of CFU-Cs decreased in the head kidney and increased in the blood and spleen of ayu infected with L. anguillarum. HSPC mobilisation after L. anguillarum infection was mediated by norepinephrine. Furthermore, HSPCs from ayu treated with L. anguillarum lipopolysaccharide (LPS) showed defective myeloid differentiation and could no longer rescue L. anguillarum-infected ayu. HSPC expansion was suppressed after L. anguillarum infection or its LPS treatment in vitro. These results reveal a link between HSPC regulation and pathogen infection in teleosts.
- Published
- 2016
6. Molecular Characterization of E-Type Prostanoid Receptor 4 (EP4) from Ayu (Plecoglossus altivelis) and Its Functional Analysis in the Monocytes/Macrophages
- Author
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Xin-Jiang Lu, Jiong Chen, and Ye-Jing Rong
- Subjects
0301 basic medicine ,Vibrio anguillarum ,Physiology ,lcsh:Medicine ,Biochemistry ,Monocytes ,White Blood Cells ,0302 clinical medicine ,Animal Cells ,Immune Physiology ,Medicine and Health Sciences ,Receptor ,lcsh:Science ,Immune Response ,Zebrafish ,Respiratory Burst ,Innate Immune System ,Multidisciplinary ,medicine.diagnostic_test ,Fishes ,Animal Models ,Complementary DNA ,Respiratory burst ,Nucleic acids ,medicine.anatomical_structure ,Cell Processes ,Osteichthyes ,Osmeriformes ,030220 oncology & carcinogenesis ,Vertebrates ,Cytokines ,lipids (amino acids, peptides, and proteins) ,Cellular Types ,Research Article ,Multiple Alignment Calculation ,Forms of DNA ,Immune Cells ,Immunology ,Spleen ,Biology ,Research and Analysis Methods ,03 medical and health sciences ,Model Organisms ,Extraction techniques ,Immune system ,Phagocytosis ,Western blot ,Computational Techniques ,Genetics ,medicine ,Animals ,Blood Cells ,Innate immune system ,Macrophages ,lcsh:R ,Organisms ,Biology and Life Sciences ,Cell Biology ,DNA ,Molecular Development ,biology.organism_classification ,Molecular biology ,Split-Decomposition Method ,RNA extraction ,030104 developmental biology ,Immune System ,lcsh:Q ,Plecoglossus altivelis ,Receptors, Prostaglandin E, EP4 Subtype ,Developmental Biology - Abstract
Prostaglandin E2 (PGE2) plays an important role in a broad spectrum of physiological and pathological processes by interacting with E-type prostanoid receptors (EPs). EP4 is one of four EP subtypes known to mediate the immune response in mammalian monocytes/macrophages. However, the precise function and characteristics of EP4 in fish remain unclear. In this study, we characterized a novel EP4-like (PaEP4L) gene from ayu, Plecoglossus altivelis. The cDNA sequence of PaEP4L is 2781 nucleotides (nts) in length, encoding a polypeptide of 459 amino acid residues with a calculated molecular weight of 51.17 kDa. Sequence comparison and phylogenetic tree analysis showed that PaEP4L shared 76% amino acid identity with that of the Atlantic salmon (Salmo salar). PaEP4L mRNA was detected by real-time quantitative PCR (QPCR) in all tested tissues and head kidney-derived monocytes/macrophages (MO/MФ). It varied greatly in liver, spleen and MO/MФ upon Vibrio anguillarum infection. Western blot analysis revealed a significant increase of PaEP4L in cell homogenates from ayu MO/MФ upon V. anguillarum infection. Moreover, anti-PaEP4L IgG reversed the down-regulation of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) mRNA expression as well as phagocytosis in ayu MO/MФ caused by PGE2. There were no significant differences in the respiratory burst response between PGE2 treated and untreated cells. We further found that cAMP mediated PGE2/PaEP4L signal in ayu MO/MФ. In conclusion, our results indicate that PaEP4L mediates PGE2 effects on ayu MO/MФ function, revealing that EP4 also plays a role in the modulation of cells of the fish's innate immune system.
- Published
- 2016
7. The assessment of depression awareness and help-seeking behaviour: experiences with the International Depression Literacy Survey
- Author
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Tracey A Davenport, Georgina Luscombe, Ye-Jing Rong, Megan L Hickie, Ian B. Hickie Am, and Morag I Bell
- Subjects
Adult ,Cross-Cultural Comparison ,Male ,China ,lcsh:RC435-571 ,media_common.quotation_subject ,education ,Ethnic group ,Context (language use) ,Literacy ,Asian People ,Surveys and Questionnaires ,lcsh:Psychiatry ,Health care ,Humans ,Mass Screening ,Medicine ,Depression (differential diagnoses) ,Mass screening ,Disease burden ,media_common ,Depression ,business.industry ,Australia ,Awareness ,Patient Acceptance of Health Care ,Health Surveys ,Help-seeking ,Psychiatry and Mental health ,Educational Status ,Female ,business ,Research Article ,Clinical psychology - Abstract
Background Depression causes substantial disease burden in both developed and developing countries. To reduce this burden, we need to promote understanding of depression as a major health condition. The International Depression Literacy Survey (IDLS) has been developed to assess understanding of depression in different cultural and health care settings. Methods Four groups of Australian university students completed the survey: medical students in second (n = 103) and fourth (n = 82) years of a graduate course, ethnic Chinese students (n = 184) and general undergraduate students (n = 38). Results Differences between the student groups were evident, with fourth year medical students demonstrating greater general health and depression literacy than second year medical students. Australian undergraduate students demonstrated better depression literacy than those from ethnic Chinese backgrounds. Ethnicity also influenced help seeking and treatment preferences (with more Chinese students being inclined to seek help from pharmacists), beliefs about discrimination and perceptions regarding stigma. Conclusion The IDLS does detect significant differences in understanding of depression among groups from different ethnic backgrounds and between those who differ in terms of prior health training. These preliminary results suggest that it may be well suited for use in a wider international context. Further investigation of the utility of the IDLS is required before these results could be extrapolated to other populations.
- Published
- 2007
8. The Prevalence of Drug Resistance Mutations Among Treatment-Naive HIV-Infected Individuals in Beijing, China
- Author
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Ye, Jing-rong, primary, Lu, Hong-yan, additional, Wang, Wei-shi, additional, Guo, Lei, additional, Xin, Ruo-lei, additional, Yu, Shuang-qing, additional, Wu, Ting-chen, additional, Zeng, Yi, additional, and He, Xiong, additional
- Published
- 2012
- Full Text
- View/download PDF
9. Genetic Diversity of HIV Type 1 Isolated from Newly Diagnosed Subjects (2006–2007) in Beijing, China
- Author
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Ye, Jing-rong, primary, Yu, Shuang-qing, additional, Lu, Hong-yan, additional, Wang, Wei-shi, additional, Xin, Ruo-lei, additional, and Zeng, Yi, additional
- Published
- 2012
- Full Text
- View/download PDF
10. Sequence Analysis of the gag–pol Gene of Human Immunodeficiency Virus Type 1 of Intersubtype (B′/C) Recombinant Strain in Beijing, China
- Author
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Ye, Jing-rong, primary, Xin, Ruo-lei, additional, Bai, Li-shi, additional, Lu, Hong-yan, additional, Yu, Shuang-qin, additional, and Zeng, Yi, additional
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- 2011
- Full Text
- View/download PDF
11. CXCR3.1 and CXCR3.2 Differentially Contribute to Macrophage Polarization in Teleost Fish.
- Author
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Xin-Jiang Lu, Qiang Chen, Ye-Jing Rong, Feng Chen, and Jiong Chen
- Subjects
- *
CHEMOKINE receptors , *OSTEICHTHYES , *FISH genomes , *ESCHERICHIA coli , *AYU , *CTENOPHARYNGODON idella - Abstract
The study of multiple copies of chemokine receptor genes in various teleosts has long appealed to investigators seeking to understand the evolution of the immune system. The CXCR CXCR3 gene has two isoforms, CXCR3.1 and CXCR3.2, which are both expressed in macrophages. The distinct roles of teleost CXCR3s have not been identified previously. In this article, we found that CXCR3.1 and CXCR3.2 differentially contributed to macrophage polarization in the teleosts: ayu (Plecoglossus altivelis), grass carp (Ctenopharyngodon idella), and spotted green pufferfish (Tetraodon nigroviridis). In ayu macrophages, the P. altivelis CXCR3.1 (PaCXCR3.1) gene was constitutively expressed, whereas the P. altivelis CXCR3.2 (PaCXCR3.2) gene was induced postinfection with Escherichia coli. Upon E. coli infection, PaCXCR3.1+ and PaCXCR3.2+ macrophages showed an M1 and an M2 phenotype, respectively. CXCL9- 11-like proteins mediated M1 and M2 polarization by interacting with the PaCXCR3.1 and PaCXCR3.2 proteins on macrophages, respectively. The transcription factors P. altivelis STAT1 and P. altivelis STAT3 were activated in PaCXCR3.1+ and PaCXCR3.2+ macrophages, respectively. Furthermore, the prognosis of septic ayu adoptively transferred with PaCXCR3.2+ macrophages was improved. Our data reveal a previously unknown mechanism for macrophage polarization, suggesting that redundant genes may regulate crucial functions in the teleost immune system. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
12. [Molecular epidemiological characteristics of HIV-1 strains isolated from newly diagnosed MSM subjects (2006-2010) in Beijing, China].
- Author
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Ye JR, Zang WC, Su XL, Lu HY, Hao MQ, Xin RL, Chen GM, He X, and Zeng Y
- Subjects
- Adult, China epidemiology, Epidemics, Genetic Variation, HIV Infections diagnosis, HIV Infections epidemiology, HIV-1 classification, Homosexuality, Male statistics & numerical data, Humans, Male, Middle Aged, Molecular Epidemiology, Molecular Sequence Data, Phylogeny, Young Adult, HIV Infections virology, HIV-1 genetics, HIV-1 isolation & purification
- Abstract
This study aims to analyze the molecular epidemiological characteristics of HIV-1 strains prevailing among men who have sex with men (MSM) in Beijing, China. The pol gene fragments from 250 newly diagnosed HIV-1-infected MSM individuals during 2006-2010 in Beijing were amplified by RT-nested PCR, sequenced, and phylogenetically analyzed. HIV-1 pol gene from 189 individuals were amplified and analyzed; 81 (42. 9%), 3 (1. 6%), 2 (1.0%), 88 (46. 6%), and 15 (7.9%) individuals were infected with HIV-1 subtypes B, B', C, CRF01_AE, and CRF07_BC, respectively. The subtypes B and CRF01_AE could both be grouped into two clusters, and CRFO7_BC strains shared high homology and were presumed to originate from a common ancestor. The HIV-1 circulating in MSM in Beijing had a lower genetic diversity than in heterosexuals. The HIV-1 epidemic (2006-2010) in MSM in Beijing was actually a rapid spread of HIV-1 CRF01 AE and B, or rather native strains of the two viruses.
- Published
- 2014
13. [Molecular epidemiological characteristics of HIV-1 B'/C strains in Beijing].
- Author
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Ye JR, Dong XG, Lu HY, Hao MQ, Su XL, Xin RL, Yu SQ, Feng X, Yan H, He X, and Zeng Y
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- Adolescent, Adult, Aged, China epidemiology, Female, HIV-1 classification, HIV-1 isolation & purification, Humans, Male, Middle Aged, Molecular Epidemiology, Phylogeny, Polymerase Chain Reaction, Young Adult, HIV Infections epidemiology, HIV Infections virology, HIV-1 genetics
- Abstract
Objective: To analyze the molecular epidemiological characteristic of HIV-1 B'/C strains prevalent in Beijing., Methods: Plasma samples were collected from 200 newly diagnosed HIV-1 B'/C individuals reported during 2006 to 2010 in Beijing. The gag gene fragments were amplified from RNA template extracted from plasma with reverse transcription (RT) and nested polymerase chain reaction (PCR). And the sequences were analyzed by phylogenetic methods and Entropy analysis., Results: A total of 159 sequences were successfully amplified from the gag genes of which 147 was CRF07_BC and 12 CRF08_BC. There were 3 main sub-clusters in CRF07_BC phylogenetic tree and they were named as sub-cluster IDU-Max (89 sequences), sub-cluster IDU-Min (22 sequences) and sub-cluster MSM (34 sequences) based on transmission.No international reference strain was closely related with these three sub-clusters except for one strain identified in Taiwan. All CRF07_BC recombinant strains were remarkable for their low interpatient diversity in gag genes (3.7%, 3.3% and 2.0% for isolates from IDU-Max, IDU-Min and MSM respectively).When compared with sub-cluster IDU-Max, there were 32 and 41 significantly different sites of nucleotide polymorphism compositions in sub-clusters IDU-Min and MSM., Conclusion: This is the first report of describing the existence of three main epidemic sub-clusters in CRF07_BC strains prevalent in Beijing. And IDU-Max sub-cluster is the dominant strain. The CRF07_BC in Beijing are less diverse than other strains and may be derived from a common ancestor.
- Published
- 2013
14. [Identification of Env-specific monoclonal antibodies from Chinese HIV-1 infected person by magnetic beads separating B cells and single cell RT-PCR cloning].
- Author
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Huang XY, Yu SQ, Cheng Z, Ye JR, Xu K, Feng X, and Zeng Y
- Subjects
- Amino Acid Sequence, Molecular Sequence Data, Antibodies, Monoclonal isolation & purification, B-Lymphocytes immunology, HIV Antibodies isolation & purification, HIV Envelope Protein gp120 immunology, HIV-1 immunology, Immunomagnetic Separation methods, Reverse Transcriptase Polymerase Chain Reaction methods
- Abstract
Objective: To establish a simple and practical method for screening of Env-specific monoclonal antibodies from HIV-1 infected individuals., Methods: Human B cells were purified by negative sorting from PBMCs and memory B cells were further enriched using anti-CD27 microbeads. Gp120 antigen labbled with biotin was incubated with memory B cells to specifically bind IgG on cells membrane. The memory B cells expressing the Env-specific antibody were harvested by magnetic beads separating, counted and diluted to the level of single cell in each PCR well that loading with catch buffer containing RNase inhibitor to get RNAs. The antibody genes were amplified by single cell RT-PCR and nested PCR, cloned into eukaryotic expression vectors and transfected into 293T cells. The binding activity of recombinant antibodies to Env were tested by ELISA., Results: Three monocolonal Env-specific antibodies were isolated from one HIV-1 infected individual., Conclusion: We can obtain Env-specific antibody by biotin labbled antigen, magnetic beads separating technique coupled with single cell RT-PCR and expression cloning.
- Published
- 2013
15. [Molecular-epidemiological characteristics of HIV-1 isolated from newly diagnosed female subjects in Beijing, 2006 - 2010].
- Author
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Ye JR, Su XL, Yu SQ, Xin RL, Hao MQ, Lu HY, Feng X, He X, and Zeng Y
- Subjects
- Adolescent, Adult, Aged, Child, Child, Preschool, China epidemiology, Female, HIV-1 isolation & purification, Humans, Middle Aged, Molecular Epidemiology, Phylogeny, Young Adult, Acquired Immunodeficiency Syndrome epidemiology, HIV-1 genetics
- Abstract
Objective: To analyze the molecular-epidemiological characteristics of HIV-1 strains prevailing among female people living with HIV in Beijing., Methods: Gag gene fragments from the 100 newly diagnosed female HIV-1 infections during 2006 to 2010 in Beijing were amplified, sequenced, and phylogenetically analyzed., Results: Eighty-two HIV-1 gag gene fragments were amplified and analyzed. 1 (1.22%), 1 (1.22%), 3 (3.66%), 23 (28.05%), 8 (9.76%), 2 (2.44%), 1 (1.22%), 18 (21.95%), 3 (3.66%), 1 (1.22%), 14 (17.07%), 4 (4.88%) and 3 (3.66%) individuals were infected with HIV-1 subtypes A1, A2, B, B', C, D, G, H, CRF01_AE, CRF02_AG, CRF07_BC, CRF08_BC and B'/C recombinants respectively., Conclusion: The subtypes circulating in female HIV infections in Beijing were more diverse than in male and the proportions of B' and rare subtypes were relatively high. Surveillance programs on HIV-1 genetic diversity should be strengthened.
- Published
- 2013
16. [Genotypic resistance mutations to antiretroviral drugs in newly confirmed human immunodeficiency virus infectors in Beijing].
- Author
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Ye JR, Guo L, Lu HY, Xin RL, and Zeng Y
- Subjects
- Adolescent, Adult, China epidemiology, Female, HIV Infections epidemiology, HIV-1 genetics, Humans, Male, Middle Aged, Molecular Epidemiology, RNA, Viral genetics, Young Adult, pol Gene Products, Human Immunodeficiency Virus genetics, Anti-HIV Agents pharmacology, Drug Resistance, Viral, HIV Infections virology, HIV-1 drug effects
- Abstract
Objective: To examine the prevalence of drug resistance mutations among the treatment-naive HIV (human immunodeficiency virus) infectors living in Beijing so as to provide the basal information for clinical antiviral treatment., Methods: HIV pol genes from plasma samples of 150 treatment-naive HIV-infected patients were amplified, sequenced and phylogenetically analyzed. And the drug-resistance associated mutations in protease and reverse transcriptase regions were analyzed with Stanford University HIV Drug Resistance Database., Results: A total of 111 pol gene sequences were obtained. The overall prevalence of drug resistance was 8.1% (9/111), corresponding to 3.6% (4/111) for protease inhibitors, 1.8% (2/111) for nucleoside reverse transcriptase inhibitors and 3.6% (4/111) for non-nucleoside reverse transcriptase inhibitors. No drug resistance mutation was identified in 17 intravenous drug users., Conclusion: The prevalence of drug resistance is relatively high in the newly confirmed HIV infectors in Beijing. Regular surveillance and monitoring of drug-resistant HIV should be implemented.
- Published
- 2011
17. [Genetic characteristics of HIV-1 CRF01_AE strains circulating in Beijing].
- Author
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Ye JR, Lu HY, Bai LS, Xin RL, and Zeng Y
- Subjects
- Beijing, HIV Infections epidemiology, Humans, Polymerase Chain Reaction, HIV-1 genetics, Phylogeny
- Abstract
Objective: To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in Beijing., Methods: Plasma samples were collected from the newly diagnosed HIV-1 individuals being reported during 2006 to 2008 in Beijing. Gag gene fragments were amplified from RNA template which were extracted from plasma by RT and nested PCR methods. 105 CRF01_AE sequences were analyzed by phylogcnetic methods and characterized through calculating the genetic distance and Entropy analysis., Results: There were four main sub-clusters in the phylogenetic tree. We named them as sub-clusters Homo-Max (67 sequences), Hetero (6 sequences), Mix (8 sequences) and Homo-Min (18 sequences) respectively, based on the mode of transmission. It was found that no international reference strain was closely related to the sub-cluster Homo-Max, Hetero or Homo-Min, including 91 samples. The strains in sub-cluster Mix consisting 8 cases that were closely related to the strains identified in Thailand and Vietnam. Genetic distance analysis on gag genes showed that the diversity of sub-clusters Homo-Max and Homo-Min was obviously less than that of the sub-cluster Hetero or Mix. When compared with sub-cluster Mix, there were 37, 29 and 11 significantly different nucleotides polymorphism compositions sites in sub-clusers Homo-Max Homo-Min and Hetero., Conclusion: This was the first report describing that four main epidemic sub-clusters were existed in CRF01_AE strains prevailing in Beijing. The virus with sub-cluster Homo-Max was the dominant strain in this region with shorter period of circulation and higher proportion seen in the HIV-infected persons. The virus in sub-cluster Mix was highly homologic with the CRF01_AE strains from Thailand and Vietnam.
- Published
- 2010
18. [Subtype and sequence analysis of gag genes among HIV-1 strains circulating in Beijing during 2007].
- Author
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Ye JR, Li Y, and Bai LS
- Subjects
- Adolescent, Adult, China epidemiology, Genotype, HIV Infections epidemiology, HIV-1 classification, Humans, Male, Middle Aged, Molecular Epidemiology, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Young Adult, HIV Infections virology, HIV-1 genetics, HIV-1 isolation & purification, gag Gene Products, Human Immunodeficiency Virus genetics
- Abstract
To investigate the subtype distribution and sequence characteristics of HIV-1 strains prevalent in Beijing during 2007 and to analyze the relationship between distribution of HIV-1 subtypes and transmission routes, we collected the anti-conglutinated whole blood samples from HIV-1 newly infected individuals in Beijing during 2007 and separated plasma specimens from the aamples. RNAs were extracted and the gag genes from the various samples were amplified by RT-PCR and nest-PCR. The PCR products were sequenced directly and phylogenetic analyses of gag genes were performed using the MEGA2 software. Among 200 HIV-1 plasma samples,161 gag HIV-1 gene fragments were amplified and analyzed. Seven HIV-1 subtypes or circulating recombinant forms (CRFs) of HIV-1 including A1 (1 strains), B (35 strains), Thai B (19 strains), C (3 strains), CRF01_AE (49 strains), CRF07_BC (51 strains), CRF08_BC (3 strain) were identified circulating in Beijing. The gene divergences inside the subtypes were different, with 7.7%, 6.5%, 6.7%, 6.7%, 5.5%, 4.3%, 5.8%, in subtype A1, B, Thai B, C, CRF01_AE, CRF07_BC, CRF08_BC, respectively. Subtypes CRF07_BC and CRF01_AE were predominant in Beijing account for 31.7% and 30.4% among samples. Seven HIV-1 subtypes exist in Beijing and the surveillance of HIV-1 gene variation should be paid more attention.
- Published
- 2009
19. [Viral load test conducive to excluding negative subjects from suspects in HIV antibody detections].
- Author
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Hei FX, Zhang QY, Sun WD, Zhang Q, Ye JR, Liu HL, and Lu HY
- Subjects
- HIV Antibodies blood, HIV Infections blood, Humans, AIDS Serodiagnosis, HIV Infections diagnosis, Viral Load
- Abstract
Objective: To study whether plasma viral load testing is helpful to exclude ones free from Human immunodeficiency virus (HIV) infections from suspects in HIV antibody detections., Methods: 19 Specimens, which showed disconcordant results of the two HIV EIA testing (S/CO < 6) and indeterminated results of Western blot (WB) test, were selected. Viral load of the specimens were detected. A six-month follow up survey in detecting HIV antibody was conducted in these subjects., Results: None of these 19 cases was observed to be positive HIV viral loads and there was no any progress in WB bands development during the follow-up period. The possibility of HIV infection could be excluded., Conclusion: When the specimens react with very low intensity in both EIA and WB, negative viral load result is conducive to exclude negative subjects from suspects in HIV antibody detections.
- Published
- 2008
20. Field evaluation of alternative testing strategies for the detection of HIV infection in Beijing.
- Author
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Hei FX, Jiang Y, Sun WD, Zhang QY, Zhang Q, Ye JR, Liu HL, Lu HY, and He X
- Subjects
- China, Humans, Sensitivity and Specificity, Blotting, Western methods, Enzyme-Linked Immunosorbent Assay methods, HIV Infections diagnosis
- Abstract
Objective: To identify a cost-efficient alternative antibody testing strategy for screening and confirmation of HIV infection by rapid simple tests (RSTs) and enzyme-linked immunosorbent assays (ELISAs)., Methods: Four RSTs (RST1, RST2, RST3, and RST4) and five ELISAs (ELISA1, ELISA2, ELISA3, ELISA4, and ELISA5) were evaluated in two phases by using banked and serum specimens prospectively collected at regional hospitals and voluntary counseling and testing (VCT) centers in Beijing. A total of 200 banked serum specimens were included in the first phase, including 62 HIV-positive, 127 HIV-negative and 11 indeterminate specimens. All specimens were tested by four RSTs and five ELISAs respectively. The second phase involved prospective testing of 389 routine specimens, including 92 HIV-positive, 287 HIV-negative, and 10 indeterminate specimens. All the specimens were tested by two RSTs (RST2 and RST4) and three ELISAs (ELISA1, ELISA3, and ELISA4), which were selected for their respective excellent sensitivity and/or specificity. Western blot (WB) was used as a gold standard for confirming the reactivity of all the specimens., Results: Sensitivity, specificity, and efficacy were calculated for each assay in two phases. In the first phase, four assays (ELISA4, RST2, RST3, and RST4) had a specificity of 100%. For the determination of efficacy, ELISA4, RST2, and RST4 were selected in the second phase. ELISA1 and ELISA3 which have a sensitivity of 95.9% and 93.2% respectively also entered this phase. In the second phase, all the five assays (ELISA1, ELISA3, ELISA4, RST2, and RST4) had a sensitivity and specifity of over 90%. ELISA1 had a sensitivity of 99% and ELISA4 a specificity of 99%., Conclusion: The sensitivity ELISA1 and the specificit of ELISA4 are comparable to ELISA/WB standard strategy. Application of this alternative testing strategy provides a cost-effective method for determining HIV prevalence in Beijing.
- Published
- 2007
21. [Subtype and sequence analysis of gag and env genes among HIV-1 strains circulating in Beijing residents during 2006].
- Author
-
Ye JR, Xing H, Liu HL, Hei FX, Zhao YJ, Liu SY, Sun WD, Zhang QY, Zhang Q, Lu HY, He X, and Shao YM
- Subjects
- China, Humans, Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, HIV-1 classification, HIV-1 genetics, env Gene Products, Human Immunodeficiency Virus genetics, gag Gene Products, Human Immunodeficiency Virus genetics
- Abstract
Objective: To investigate the subtype distribution and the prevalence of sequence characteristics of HIV-1 strains in Beijing residents during 2006 and to analyze the relationship between distribution of HIV-1 subtypes and transmission routines., Methods: Blood samples from 32 new confirmed HIV-1 infected individuals from Beijing residents in 2006 and separated plasma specimens were collected. RNAs were extracted and the gag and env gene were amplified by RT-PCR and nest-PCR. PCR products were sequenced directly and phylogenetic analyses of gag and env gene were performed using the MEGA2 software., Results: Among 32 HIV-1 plasma samples, 22 gag and 4 env gene fragments were amplified and analyzed. Five HIV-1 subtypes or circulating recombinant forms(CRFs) of HIV-1 including Thai B (2 strains), B (9 strains), C (2 strains), CRF07_BC (5 strains), CRF01 AE (4 strains) were identified being circulated in Beijing. The gene divergences of gag gene inside the subtypes were 6.6%, 4.3%, 6.8%, 4.9% and 3.0% in subtype B, Thai B, C, CRF01_AE and CRF07_BC respectively. Subtypes B were predominant in Beijing, accounted for 40.9% among 22 samples., Conclusion: Five HIV-1 subtypes were identified in Beijing and the surveillance of HIV-1 gene variation should be paid more attention to.
- Published
- 2007
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